MD10G1184500.v1.1

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0004513
Physical Location & Seq
Forward (+)
27755267 .. 27757615
2349 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1184500.v1.1.491

Sequence Viewer

Length: 450 bp
ATGGCTGCAATGTCTTTCAGAGGGCTCAGTCGGCCAAATGCTTCATGTGCCATGGGGGTTTCTGATGAGAGCAAGAACACTTTCATGGAGCTGCAGAGAAAGAAGGTTCACCGCTATGTGATATTCAAGGTTGAGGAGAAGAAGAGGGAGGTCGTAGTTGAAAAGATTGGTGGTCCGGCGGAGAGCTATGATGATTTTGTGGCAGCTTTGCCTGATAATGATTGTCGATATGCCGTATATGACTTTGATTTCGTAACTTCTGAGAACTGTCAAAAGAGCAAGATCTTCTTCATCGCATGGTCCCCTTCGACCTCTCGAATCCGTGCGAAGATGCTCTACGCCACATCTAAAGACAGGTTTAGGAGGGAGCTGGACGGTATCCACTACGAGATTCAGGCTACTGACCCAACAGAGATGGATCTTGAGGTGCTCAGAGACCGTGCCCATTGA

Protein Analysis

150

Amino Acids

17.25

Weight (kDa)

6.83

Isoelectric Point (pI)

47.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 18 - 149 4.3e-42 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 112, 179
AclWI GGATC 1 cut(s) 426
AcoI YGGCCR 1 cut(s) 32
AgsI TTSAA 2 cut(s) 127, 161
AluBI AGCT 4 cut(s) 91, 186, 206, 370
AluI AGCT 4 cut(s) 91, 186, 206, 370
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 1 cut(s) 426
AoxI GGCC 1 cut(s) 32
ApeKI GCWGC 3 cut(s) 5, 91, 203
ArsI GACNNNNNNTTYG 2 cut(s) 233, 265
Asp700I GAANNNNTTC 1 cut(s) 80
AspS9I GGNCC 2 cut(s) 173, 300
AsuHPI GGTGA 1 cut(s) 101
AvaII GGWCC 2 cut(s) 173, 300
BaeGI GKGCMC 1 cut(s) 445
BanII GRGCYC 1 cut(s) 27
BarI GAAGNNNNNNTAC 2 cut(s) 320, 352
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 2 cut(s) 78, 215
BccI CCATC 1 cut(s) 409
BceAI ACGGC 1 cut(s) 218
BciVI GTATCC 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 92
BfuI GTATCC 1 cut(s) 389
BglII AGATCT 1 cut(s) 282
BisI GCNGC 3 cut(s) 6, 92, 204
BlsI GCNGC 3 cut(s) 7, 93, 205
Bme18I GGWCC 2 cut(s) 173, 300
BmgT120I GGNCC 2 cut(s) 173, 300
BmiI GGNNCC 1 cut(s) 302
BmsI GCATC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 429
BsaJI CCNNGG 1 cut(s) 51
Bse3DI GCAATG 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 51
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 3 cut(s) 40, 252, 445
BseRI GAGGAG 1 cut(s) 149
BseSI GKGCMC 1 cut(s) 445
BseXI GCAGC 2 cut(s) 78, 215
BshFI GGCC 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 432
BsiSI CCGG 1 cut(s) 176
BslFI GGGAC 1 cut(s) 286
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 286
BsnI GGCC 1 cut(s) 34
Bso31I GGTCTC 1 cut(s) 429
Bsp1286I GDGCHC 3 cut(s) 27, 432, 445
Bsp143I GATC 2 cut(s) 282, 418
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 2 cut(s) 112, 179
BspANI GGCC 1 cut(s) 34
BspCNI CTCAG 3 cut(s) 39, 253, 444
BspLI GGNNCC 1 cut(s) 302
BspMAI CTGCAG 1 cut(s) 96
BspPI GGATC 1 cut(s) 426
BspTNI GGTCTC 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 51
BssMI GATC 2 cut(s) 282, 418
BssT1I CCWWGG 1 cut(s) 51
Bst4CI ACNGT 3 cut(s) 269, 377, 440
Bst6I CTCTTC 1 cut(s) 137
BstDEI CTNAG 3 cut(s) 26, 261, 431
BstDSI CCRYGG 1 cut(s) 51
BstKTI GATC 2 cut(s) 285, 421
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 2 cut(s) 282, 418
BstMWI GCNNNNNNNGC 2 cut(s) 31, 47
BstSFI CTRYAG 1 cut(s) 92
BstSLI GKGCMC 1 cut(s) 445
BstV1I GCAGC 2 cut(s) 78, 215
BstX2I RGATCY 2 cut(s) 282, 418
BstYI RGATCY 2 cut(s) 282, 418
BsuI GTATCC 1 cut(s) 389
BsuRI GGCC 1 cut(s) 34
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 277
Cfr13I GGNCC 2 cut(s) 173, 300
CviAII CATG 4 cut(s) 45, 52, 85, 297
CviJI RGCY 8 cut(s) 5, 25, 34, 91, 186, 206, 370, 398
CviKI_1 RGCY 8 cut(s) 5, 25, 34, 91, 186, 206, 370, 398
DdeI CTNAG 3 cut(s) 26, 261, 431
DpnI GATC 2 cut(s) 284, 420
DpnII GATC 2 cut(s) 282, 418
EaeI YGGCCR 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
EciI GGCGGA 1 cut(s) 194
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 429
Eco47I GGWCC 2 cut(s) 173, 300
EcoT14I CCWWGG 1 cut(s) 51
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 4 cut(s) 48, 55, 88, 300
FaiI YATR 9 cut(s) 46, 53, 86, 117, 189, 231, 238, 240, 298
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 1 cut(s) 286
FatI CATG 4 cut(s) 44, 51, 84, 296
Fnu4HI GCNGC 3 cut(s) 6, 92, 204
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 3 cut(s) 6, 92, 204
GluI GCNGC 3 cut(s) 6, 92, 204
HaeIII GGCC 1 cut(s) 34
HapII CCGG 1 cut(s) 176
Hin1II CATG 4 cut(s) 48, 55, 88, 300
HinfI GANTC 2 cut(s) 318, 391
HpaII CCGG 1 cut(s) 176
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 4 cut(s) 20, 64, 262, 434
Hpy188III TCNNGA 2 cut(s) 315, 422
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 2 cut(s) 97, 315
HpyCH4III ACNGT 3 cut(s) 269, 377, 440
HpyCH4V TGCA 2 cut(s) 8, 94
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 47
HpyF3I CTNAG 3 cut(s) 26, 261, 431
Hsp92II CATG 4 cut(s) 48, 55, 88, 300
Kzo9I GATC 2 cut(s) 282, 418
LmnI GCTCC 2 cut(s) 88, 367
LpnPI CCDG 5 cut(s) 189, 225, 340, 356, 380
Lsp1109I GCAGC 2 cut(s) 78, 215
LweI GCATC 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 253
MalI GATC 2 cut(s) 284, 420
MboI GATC 2 cut(s) 282, 418
MboII GAAGA 5 cut(s) 151, 154, 277, 280, 340
MflI RGATCY 2 cut(s) 282, 418
MhlI GDGCHC 3 cut(s) 27, 432, 445
MnlI CCTC 7 cut(s) 14, 127, 138, 142, 322, 357, 418
MroXI GAANNNNTTC 1 cut(s) 80
MslI CAYNNNNRTG 2 cut(s) 83, 114
MspI CCGG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 31, 47
NcoI CCATGG 1 cut(s) 51
NdeII GATC 2 cut(s) 282, 418
NlaIII CATG 4 cut(s) 48, 55, 88, 300
NlaIV GGNNCC 1 cut(s) 302
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 2 cut(s) 318, 391
PkrI GCNGC 3 cut(s) 7, 93, 205
PspN4I GGNNCC 1 cut(s) 302
PspPI GGNCC 2 cut(s) 173, 300
PstI CTGCAG 1 cut(s) 96
PsuI RGATCY 2 cut(s) 282, 418
RseI CAYNNNNRTG 2 cut(s) 83, 114
SatI GCNGC 3 cut(s) 6, 92, 204
Sau3AI GATC 2 cut(s) 282, 418
Sau96I GGNCC 2 cut(s) 173, 300
SduI GDGCHC 3 cut(s) 27, 432, 445
SfaNI GCATC 1 cut(s) 321
SfcI CTRYAG 1 cut(s) 92
SinI GGWCC 2 cut(s) 173, 300
SmiMI CAYNNNNRTG 2 cut(s) 83, 114
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
SsiI CCGC 2 cut(s) 112, 179
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 3 cut(s) 269, 377, 440
TaqI TCGA 3 cut(s) 226, 308, 316
TfiI GAWTC 2 cut(s) 318, 391
TseI GCWGC 3 cut(s) 5, 91, 203
TspDTI ATGAA 3 cut(s) 33, 73, 280
TspGWI ACGGA 1 cut(s) 311
VpaK11BI GGWCC 2 cut(s) 173, 300
XmnI GAANNNNTTC 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.