RchiOBHm_Chr6g0288181

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0004513
Physical Location & Seq
Forward (+)
51449933 .. 51451719
1787 bp
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UTR
Exon/CDS
Intron
PRQ25856

Sequence Viewer

Length: 441 bp
ATGTCTTTCAGAGGGCTCAGTCGGCCGAATGCTTCGTGTGCCATGGCTGCGGCTGAGCACAGCAAGAACACTTTTATGGAACTGCAGAGGAAGAAGGTTCACCGCTATGTGATATTTAGGGTGGATGAGAAGAAAAAGGAGGTTGTGGTTGAGAAGATTGGTGGTCCGGCTGAGAGTTATGATGATTTTGTGGCGGCTTTGCCGGAGAATGATTGCCGATATGCAGTATATGACTTTGATTTTGTAACCTCTGATAACTGTCAAAAGAGCAAGATCTTCTTCATTGCTTGGTCCCCTTCAACCTCCCGGATCCGTGCCAAGATGCTGTATGCCACATCGAAAGACCAATTTAGGCGGGAGCTGGATGGTATCCACTACGAGATTCAAGCTACTGACCCAACAGAGATGGATCTTGAGGTGATCAAAGAGCGTGCGCACTGA

Protein Analysis

146

Amino Acids

16.91

Weight (kDa)

6.9

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 18 - 146 4.2e-41 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 435
AciI CCGC 4 cut(s) 50, 103, 194, 355
AclWI GGATC 3 cut(s) 304, 317, 417
AcoI YGGCCR 1 cut(s) 23
AgsI TTSAA 2 cut(s) 300, 386
AluBI AGCT 2 cut(s) 361, 389
AluI AGCT 2 cut(s) 361, 389
Alw21I GWGCWC 1 cut(s) 60
AlwI GGATC 3 cut(s) 304, 317, 417
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 1 cut(s) 47
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 2 cut(s) 164, 291
AsuC2I CCSGG 1 cut(s) 307
AsuHPI GGTGA 2 cut(s) 92, 430
AvaII GGWCC 2 cut(s) 164, 291
BamHI GGATCC 1 cut(s) 309
BanII GRGCYC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 34
BccI CCATC 2 cut(s) 359, 400
BciVI GTATCC 1 cut(s) 380
BclI TGATCA 1 cut(s) 420
BcnI CCSGG 1 cut(s) 307
BfmI CTRYAG 1 cut(s) 83
BfuI GTATCC 1 cut(s) 380
BglII AGATCT 1 cut(s) 273
BisI GCNGC 3 cut(s) 48, 51, 195
BlpI GCTNAGC 1 cut(s) 54
BlsI GCNGC 3 cut(s) 49, 52, 196
Bme1390I CCNGG 1 cut(s) 307
Bme18I GGWCC 2 cut(s) 164, 291
BmgT120I GGNCC 2 cut(s) 164, 291
BmiI GGNNCC 2 cut(s) 293, 311
BmrFI CCNGG 1 cut(s) 307
BmsI GCATC 1 cut(s) 312
Bpu1102I GCTNAGC 1 cut(s) 54
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 307
BsaJI CCNNGG 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 282
BseDI CCNNGG 1 cut(s) 42
BseGI GGATG 2 cut(s) 130, 370
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 3 cut(s) 31, 45, 162
BseX3I CGGCCG 1 cut(s) 23
BseXI GCAGC 1 cut(s) 34
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 1 cut(s) 25
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 60
BsiSI CCGG 3 cut(s) 167, 203, 307
BslFI GGGAC 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 277
BsmI GAATGC 1 cut(s) 34
BsnI GGCC 1 cut(s) 25
Bsp1286I GDGCHC 2 cut(s) 18, 60
Bsp143I GATC 4 cut(s) 273, 309, 409, 420
Bsp1720I GCTNAGC 1 cut(s) 54
Bsp19I CCATGG 1 cut(s) 42
BspACI CCGC 4 cut(s) 50, 103, 194, 355
BspANI GGCC 1 cut(s) 25
BspCNI CTCAG 3 cut(s) 30, 46, 163
BspLI GGNNCC 2 cut(s) 293, 311
BspMAI CTGCAG 1 cut(s) 87
BspPI GGATC 3 cut(s) 304, 317, 417
BsrDI GCAATG 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 42
BssMI GATC 4 cut(s) 273, 309, 409, 420
BssT1I CCWWGG 1 cut(s) 42
Bst4CI ACNGT 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 3 cut(s) 17, 54, 171
BstDSI CCRYGG 1 cut(s) 42
BstF5I GGATG 2 cut(s) 130, 370
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 4 cut(s) 276, 312, 412, 423
BstMBI GATC 4 cut(s) 273, 309, 409, 420
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 3 cut(s) 22, 38, 47
BstSCI CCNGG 1 cut(s) 305
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 34
BstX2I RGATCY 3 cut(s) 273, 309, 409
BstYI RGATCY 3 cut(s) 273, 309, 409
BstZI CGGCCG 1 cut(s) 23
BsuI GTATCC 1 cut(s) 380
BsuRI GGCC 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 42
BtsCI GGATG 2 cut(s) 130, 370
BtsIMutI CAGTG 1 cut(s) 436
Cac8I GCNNGC 1 cut(s) 432
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 2 cut(s) 164, 291
CviAII CATG 1 cut(s) 43
CviJI RGCY 8 cut(s) 16, 25, 47, 53, 170, 197, 361, 389
CviKI_1 RGCY 8 cut(s) 16, 25, 47, 53, 170, 197, 361, 389
DdeI CTNAG 3 cut(s) 17, 54, 171
DpnI GATC 4 cut(s) 275, 311, 411, 422
DpnII GATC 4 cut(s) 273, 309, 409, 420
EaeI YGGCCR 1 cut(s) 23
EagI CGGCCG 1 cut(s) 23
EclXI CGGCCG 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 18
Eco47I GGWCC 2 cut(s) 164, 291
Eco52I CGGCCG 1 cut(s) 23
EcoT14I CCWWGG 1 cut(s) 42
EcoT38I GRGCYC 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 1 cut(s) 46
FaiI YATR 8 cut(s) 44, 77, 108, 180, 222, 229, 231, 330
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 1 cut(s) 277
FatI CATG 1 cut(s) 42
FauI CCCGC 1 cut(s) 348
FbaI TGATCA 1 cut(s) 420
Fnu4HI GCNGC 3 cut(s) 48, 51, 195
FokI GGATG 2 cut(s) 137, 377
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 3 cut(s) 48, 51, 195
FspAI RTGCGCAY 1 cut(s) 435
FspI TGCGCA 1 cut(s) 435
GlaI GCGC 1 cut(s) 435
GluI GCNGC 3 cut(s) 48, 51, 195
HaeIII GGCC 1 cut(s) 25
HapII CCGG 3 cut(s) 167, 203, 307
HhaI GCGC 1 cut(s) 436
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HinfI GANTC 1 cut(s) 382
HpaII CCGG 3 cut(s) 167, 203, 307
HphI GGTGA 2 cut(s) 92, 430
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 2 cut(s) 11, 253
Hpy188III TCNNGA 1 cut(s) 413
Hpy8I GTNNAC 1 cut(s) 100
HpyAV CCTTC 2 cut(s) 88, 306
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4V TGCA 2 cut(s) 85, 224
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 38, 47
HpyF3I CTNAG 3 cut(s) 17, 54, 171
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 434
Ksp22I TGATCA 1 cut(s) 420
Kzo9I GATC 4 cut(s) 273, 309, 409, 420
LmnI GCTCC 1 cut(s) 358
LpnPI CCDG 4 cut(s) 180, 216, 320, 347
Lsp1109I GCAGC 1 cut(s) 34
LweI GCATC 1 cut(s) 312
MaeIII GTNAC 1 cut(s) 244
MalI GATC 4 cut(s) 275, 311, 411, 422
MboI GATC 4 cut(s) 273, 309, 409, 420
MboII GAAGA 5 cut(s) 103, 142, 166, 268, 271
MflI RGATCY 3 cut(s) 273, 309, 409
MhlI GDGCHC 2 cut(s) 18, 60
MluCI AATT 1 cut(s) 347
MnlI CCTC 6 cut(s) 5, 81, 133, 259, 313, 409
MslI CAYNNNNRTG 2 cut(s) 74, 105
MspI CCGG 3 cut(s) 167, 203, 307
MspR9I CCNGG 1 cut(s) 307
Mva1269I GAATGC 1 cut(s) 34
MwoI GCNNNNNNNGC 3 cut(s) 22, 38, 47
NciI CCSGG 1 cut(s) 307
NcoI CCATGG 1 cut(s) 42
NdeII GATC 4 cut(s) 273, 309, 409, 420
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 2 cut(s) 293, 311
NsbI TGCGCA 1 cut(s) 435
PctI GAATGC 1 cut(s) 34
PfeI GAWTC 1 cut(s) 382
PfoI TCCNGGA 1 cut(s) 305
PkrI GCNGC 3 cut(s) 49, 52, 196
PspN4I GGNNCC 2 cut(s) 293, 311
PspPI GGNCC 2 cut(s) 164, 291
PstI CTGCAG 1 cut(s) 87
PsuI RGATCY 3 cut(s) 273, 309, 409
RseI CAYNNNNRTG 2 cut(s) 74, 105
SatI GCNGC 3 cut(s) 48, 51, 195
Sau3AI GATC 4 cut(s) 273, 309, 409, 420
Sau96I GGNCC 2 cut(s) 164, 291
ScrFI CCNGG 1 cut(s) 307
SduI GDGCHC 2 cut(s) 18, 60
SetI ASST 7 cut(s) 99, 144, 251, 305, 363, 391, 420
SfaNI GCATC 1 cut(s) 312
SfcI CTRYAG 1 cut(s) 83
SinI GGWCC 2 cut(s) 164, 291
SmiMI CAYNNNNRTG 2 cut(s) 74, 105
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
Sse9I AATT 1 cut(s) 347
SsiI CCGC 4 cut(s) 50, 103, 194, 355
StyD4I CCNGG 1 cut(s) 305
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 1 cut(s) 260
TaqI TCGA 1 cut(s) 338
TasI AATT 1 cut(s) 347
TauI GCSGC 2 cut(s) 53, 197
TfiI GAWTC 1 cut(s) 382
TseI GCWGC 1 cut(s) 47
TspDTI ATGAA 1 cut(s) 271
TspGWI ACGGA 1 cut(s) 302
VpaK11BI GGWCC 2 cut(s) 164, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.