FvH4_2g24460

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006473
Physical Location & Seq
Forward (+)
19952079 .. 19960134
8056 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g24460.t1

Sequence Viewer

Length: 2652 bp
ATGAGTTCGAAGCCCTGGCTGTACCCGGCTCCCACCTACCGTACTCTCGAGACCTATTGGGACACTGACGACGACGCGCCCGGCCCGCGATGCGGTCACACGCTCACCGCCGTCGCCGCCACCAAGACCCACGGCCCCCGTCTTATTCTCTTCGGTGGCGCCACTGCCATTGAAGGCGGCGCCGCCTCCTCCGCCCCCGGCATCCGGTTAGCGGGTGTGACCAATTCTGTTCATTCCTACGATGTTCTCACCAGAAAGTGGACTAGAATCAGGCCGGCTGGTGAGCCACCGTCTCCTAGGGCGGCCCACGCGGCAGCTGCAGTTGGCACTATGGTGGTATTTCAGGGCGGCATTGGTCCGGCTGGGCACTCCACGGATGACCTCTATGTGCTTGATATGGATAAGTTCAAGTGGCACAGAGTGGTTGTGCAAGGACAAGGACCTGGGCCTCGCTATGGCCATGTGATGGACTTAGTTGGTCAGAGATATCTTGTTACTGTCAGCGGGAATGATGGAAAAAGAGTTCTTTCTGATTCCTGGGCTCTGGACACTGCACAGAAACCTTACGCATGGGCTAAGCTGAGCCCAGAAGGGGATAGGCCTTCTGCTAGAATGTATGCAACAGCCAGTGCCCGCTCAGATGGCATGTTTTTGCTTTGTGGCGGAAGAGACGCTTCTGGGGCACCCCTATCAGATGCTTATGGATTGCTCATGCATAGGAATGGTCAGTGGGAGTGGACTCTTGCACCCGGAGTGTCCCCTTCACCAAGATATCAACATGCTGCGGTTTTTGTTGGTGCAAGATTACATGTAACTGGAGGTGCTCTTAGGGGAGGACGTGCTGTAGAAGGTGAAGGAGCTATTGCAGTTTTGGACACTGCCGCTGGAGTTTGGTTAGATAGAAGCGGGCTTGTGACCTCCTCACGGACAAGCAAGGGACAGAATGACTATGATCCCCCTTTGGAGCTTATGCGTCGTTGTCGCCATGCAGCCGCATCTCTTGGTGTTCGTATATACATTTATGGTGGTCTCAAGGGAGATATCTTGTTAGATGATTTCCTGGTTGCGGAAAATTCTTCATTTCAATCTGAAGTTAATTCTCCCGTATCAACTTCTGAGAAATCCCCATCTGTACCGAGAGCCTTTCATGCCAACACTACTTCTTTTGGTGTATCACCATCTTCGGATGGTGAATCTGAGATACCCTCATCTAGTGGCTTGAGCATGGATAAAAATTCCATGGAGAAACTACGGCAGGCTTCTGCTGCTGAAGCTGAGGCAGCTAGTGCTGTTTGGCAAGCTGTGCAGTCCCAATCTTCCCCTCCTCCTGAAGAAACATCTGTTTCAGATGACAACTCACAAATTGCAGAAACACTTTCAGATGGTAGTGACACTGAGGCAGATGTTCGCCTTCATCCCAGAGCTGTTGTTGTTGCCAAGGAGGCTGTAGGCAACTTGGGTGGCATGGTCAGACAGTTGTCTTTGGATCAATTTGAAAACGAGAGCAGACGAATGATTCCAATGAATAACGACTTATCATATCCTAACAAAAAGTTTACCAGGCAGAAGTCCCCACAGGGCCTGCATAAGAAGATAATATCTACATTACTGAGGCCAAGGAACTGGAAACCTCCGGCTAATAGGAAGTTTTTCCTCGATTCTTACGAAGTGGGAGAACTTTGTTATGCTGCTGAACAGATCTTTATGCAGGAGCAAACAGTTCTTCAGCTGAAAGCTCCTGTTAAAGTTTTTGGCGATCTTCATGGACAGTTTGGTGATTTAATGCGCTTGTTTGATGAATATGGATTTCCATCTGCCGCAGGAGACATAACGTACATTGATTACTTGTTTTTGGGGGATTATGTTGATCGAGGACAGCACAGCTTGGAGACAATAACTCTACTTCTAGCACTAAAGATTGAGTATCCCGATAATGTTCACTTAATACGTGGAAACCATGAGGCTGCTGATATCAATGCACTCTTTGGTTTTCGTATTGAATGCATTGAGAGAATGGGAGAGAGTGATGGAATATGGGCATGGACGCGGTTCAATCAACTTTTCAACTACCTCCCATTGGCTGCACTAATTGAAAAGAAAATCATCTGTATGCATGGTGGCATTGGGAGATCTATAAATTCTGTGGAACAAATAGAGAAGCTTGAAAGACCCATTACGATGGATGCTGGGTCTATAATTTTAATGGATCTTCTATGGTCTGATCCTACAGAAAATGATGGTGTAGAGGGTCTCAGACCAAATGCAAGAGGGCCTGGTCTTGTCACTTTCGGGCCTGATCGTGTCATGGACTTTTGTAAGAGGAACAAATTACAGCTCATTATAAGAGCTCATGAATGTGTTATGGATGGTTTTGAACGGTTTGCCCAGGGACAACTGATCACTCTATTTTCGGCCACCAACTATTGTGGAACGGCAAACAATGCTGGTGCAATACTGGTGGTTGGCAGAGGGTTGGTTGTAGTTCCAAAACTAATTCATCCTTTGCCACCTCCGATTCAGTCACCGGAGACATCTCCTGAACGTGTCATAGATGACACATGGATGCAGGAGCTAAACATTCAGAGGCCACCAACTCCTACTCGTGGTCGGCCACAGCCTGATCTTGATCGGAACTCACTTGCATATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4 Metallophos STPPase_N

Protein Analysis

884

Amino Acids

96.37

Weight (kDa)

6.0

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 21 - 217 9e-33 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 22 - 186 7.9e-26 FBX42, beta-propeller domain
Kelch_HCF PF13854 72 - 346 2e-16 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 74 - 350 1.3e-20 Attractin/LZTR1 beta-propeller
Beta-prop_FBX42 PF13415 176 - 355 4.1e-12 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 201 - 277 5.9e-07 tRNA wybutosine-synthesizing protein 4, beta-propeller
Metallophos PF00149 580 - 787 1.9e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2342
AccB1I GGYRCC 3 cut(s) 158, 179, 682
AccB7I CCANNNNNTGG 2 cut(s) 258, 466
AccBSI CCGCTC 1 cut(s) 636
AccII CGCG 4 cut(s) 77, 88, 311, 2047
AclWI GGATC 4 cut(s) 947, 1494, 2214, 2216
AcoI YGGCCR 3 cut(s) 457, 2412, 2609
AcsI RAATTY 3 cut(s) 1072, 1234, 2137
AcuI CTGAAG 4 cut(s) 1110, 1290, 1350, 1709
AcyI GRCGYC 2 cut(s) 159, 180
AdeI CACNNNGTG 1 cut(s) 421
AfaI GTAC 4 cut(s) 23, 43, 1134, 1835
AflIII ACRYGT 2 cut(s) 808, 2542
AjiI CACGTC 1 cut(s) 839
AjnI CCWGG 7 cut(s) 14, 442, 536, 1059, 1559, 2272, 2385
AleI CACNNNNGTG 1 cut(s) 332
AloI GAACNNNNNNTCC 2 cut(s) 507, 539
Alw21I GWGCWC 2 cut(s) 826, 2350
Alw26I GTCTC 8 cut(s) 44, 297, 663, 1034, 1818, 1883, 2255, 2522
AlwI GGATC 4 cut(s) 947, 1494, 2214, 2216
AlwNI CAGNNNCTG 1 cut(s) 1582
Ama87I CYCGRG 1 cut(s) 47
ApeKI GCWGC 9 cut(s) 314, 317, 782, 989, 1265, 1280, 1688, 1964, 2081
ApoI RAATTY 3 cut(s) 1072, 1234, 2137
Asp700I GAANNNNTTC 1 cut(s) 1649
AspA2I CCTAGG 1 cut(s) 296
AspLEI GCGC 4 cut(s) 79, 161, 182, 1788
AspS9I GGNCC 9 cut(s) 83, 134, 304, 356, 440, 446, 1579, 2270, 2291
AsuC2I CCSGG 4 cut(s) 26, 81, 198, 750
AsuHPI GGTGA 9 cut(s) 97, 241, 293, 756, 863, 1167, 1202, 1787, 2514
AsuII TTCGAA 1 cut(s) 8
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 2 cut(s) 356, 440
AvrII CCTAGG 1 cut(s) 296
BaeGI GKGCMC 3 cut(s) 369, 634, 685
BalI TGGCCA 1 cut(s) 459
BanI GGYRCC 3 cut(s) 158, 179, 682
BanII GRGCYC 3 cut(s) 544, 587, 2350
BauI CACGAG 1 cut(s) 2601
Bbv12I GWGCWC 2 cut(s) 826, 2350
BbvCI CCTCAGC 1 cut(s) 1275
BbvI GCAGC 9 cut(s) 304, 326, 769, 1001, 1252, 1292, 1675, 1951, 2068
BceAI ACGGC 4 cut(s) 95, 148, 1268, 2448
BciT130I CCWGG 7 cut(s) 16, 444, 538, 1061, 1561, 2274, 2387
BciVI GTATCC 1 cut(s) 1935
BclI TGATCA 1 cut(s) 2397
BcnI CCSGG 4 cut(s) 26, 81, 198, 750
BcoDI GTCTC 8 cut(s) 44, 297, 663, 1034, 1818, 1883, 2255, 2522
BfaI CTAG 6 cut(s) 264, 297, 609, 1212, 1284, 1907
BfmI CTRYAG 4 cut(s) 318, 843, 1446, 2226
BfoI RGCGCY 2 cut(s) 162, 183
BfuI GTATCC 1 cut(s) 1935
BglI GCCNNNNNGGC 2 cut(s) 311, 1442
BglII AGATCT 2 cut(s) 1698, 2129
BlnI CCTAGG 1 cut(s) 296
BlpI GCTNAGC 2 cut(s) 576, 581
Bme18I GGWCC 2 cut(s) 356, 440
BmeT110I CYCGRG 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 839
BmgT120I GGNCC 9 cut(s) 83, 134, 304, 356, 440, 446, 1579, 2270, 2291
BmiI GGNNCC 5 cut(s) 30, 136, 160, 181, 684
BmsI GCATC 6 cut(s) 80, 210, 685, 1004, 2173, 2553
BoxI GACNNNNGTC 1 cut(s) 1477
BplI GAGNNNNNCTC 2 cut(s) 1190, 1222
BpmI CTGGAG 2 cut(s) 837, 906
Bpu10I CCTNAGC 1 cut(s) 1275
Bpu1102I GCTNAGC 2 cut(s) 576, 581
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 2 cut(s) 1016, 1240
BpuMI CCSGG 4 cut(s) 26, 81, 198, 750
BsaAI YACGTR 1 cut(s) 1949
BsaBI GATNNNNATC 2 cut(s) 1810, 2625
BsaHI GRCGYC 2 cut(s) 159, 180
BsaI GGTCTC 3 cut(s) 44, 1034, 2255
BsaWI WCCGGW 2 cut(s) 204, 2524
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 4 cut(s) 627, 820, 1628, 2460
Bse8I GATNNNNATC 2 cut(s) 1810, 2625
BseBI CCWGG 7 cut(s) 16, 444, 538, 1061, 1561, 2274, 2387
BseGI GGATG 8 cut(s) 201, 382, 1192, 1414, 2188, 2371, 2497, 2568
BseJI GATNNNNATC 2 cut(s) 1810, 2625
BseMII CTCAG 8 cut(s) 572, 651, 1107, 1188, 1266, 1386, 1601, 2266
BseNI ACTGG 4 cut(s) 627, 820, 1628, 2460
BseRI GAGGAG 3 cut(s) 178, 910, 1314
BseSI GKGCMC 3 cut(s) 369, 634, 685
BseXI GCAGC 9 cut(s) 304, 326, 769, 1001, 1252, 1292, 1675, 1951, 2068
BseYI CCCAGC 2 cut(s) 362, 2186
BsgI GTGCAG 3 cut(s) 537, 1325, 2067
Bsh1236I CGCG 4 cut(s) 77, 88, 311, 2047
BshNI GGYRCC 3 cut(s) 158, 179, 682
BsiHKAI GWGCWC 2 cut(s) 826, 2350
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 9 cut(s) 26, 81, 198, 205, 275, 359, 750, 1634, 2525
BslFI GGGAC 6 cut(s) 74, 742, 951, 1294, 1555, 2403
BsmAI GTCTC 8 cut(s) 44, 297, 663, 1034, 1818, 1883, 2255, 2522
BsmBI CGTCTC 2 cut(s) 297, 663
BsmFI GGGAC 6 cut(s) 74, 742, 951, 1294, 1555, 2403
BsmI GAATGC 1 cut(s) 2006
Bso31I GGTCTC 3 cut(s) 44, 1034, 2255
BsoBI CYCGRG 1 cut(s) 47
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 7 cut(s) 369, 544, 587, 634, 685, 826, 2350
Bsp1720I GCTNAGC 2 cut(s) 576, 581
Bsp19I CCATGG 1 cut(s) 1239
BspCNI CTCAG 8 cut(s) 573, 650, 1108, 1189, 1267, 1387, 1602, 2265
BspFNI CGCG 4 cut(s) 77, 88, 311, 2047
BspHI TCATGA 1 cut(s) 2350
BspLI GGNNCC 5 cut(s) 30, 136, 160, 181, 684
BspMAI CTGCAG 1 cut(s) 322
BspPI GGATC 4 cut(s) 947, 1494, 2214, 2216
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 3 cut(s) 158, 179, 682
BspTNI GGTCTC 3 cut(s) 44, 1034, 2255
BsrBI CCGCTC 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 4 cut(s) 627, 820, 1628, 2460
BssAI RCCGGY 1 cut(s) 274
BssNI GRCGYC 2 cut(s) 159, 180
BssSI CACGAG 1 cut(s) 2601
BssT1I CCWWGG 4 cut(s) 296, 1239, 1437, 1616
Bst2BI CACGAG 1 cut(s) 2601
Bst2UI CCWGG 7 cut(s) 16, 444, 538, 1061, 1561, 2274, 2387
Bst4CI ACNGT 7 cut(s) 41, 291, 499, 1476, 1720, 1770, 2379
Bst6I CTCTTC 2 cut(s) 155, 661
BstACI GRCGYC 2 cut(s) 159, 180
BstAPI GCANNNNNTGC 3 cut(s) 1286, 1303, 2441
BstBAI YACGTR 1 cut(s) 1949
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 7 cut(s) 86, 276, 634, 908, 1257, 1299, 1583
BstDSI CCRYGG 3 cut(s) 130, 372, 1239
BstF5I GGATG 8 cut(s) 201, 382, 1192, 1414, 2188, 2371, 2497, 2568
BstFNI CGCG 4 cut(s) 77, 88, 311, 2047
BstH2I RGCGCY 2 cut(s) 162, 183
BstHHI GCGC 4 cut(s) 79, 161, 182, 1788
BstMAI GTCTC 8 cut(s) 44, 297, 663, 1034, 1818, 1883, 2255, 2522
BstNI CCWGG 7 cut(s) 16, 444, 538, 1061, 1561, 2274, 2387
BstNSI RCATGY 3 cut(s) 649, 782, 812
BstPAI GACNNNNGTC 1 cut(s) 1477
BstSFI CTRYAG 4 cut(s) 318, 843, 1446, 2226
BstSLI GKGCMC 3 cut(s) 369, 634, 685
BstUI CGCG 4 cut(s) 77, 88, 311, 2047
BstV1I GCAGC 9 cut(s) 304, 326, 769, 1001, 1252, 1292, 1675, 1951, 2068
BstX2I RGATCY 3 cut(s) 1698, 2129, 2206
BstXI CCANNNNNNTGG 2 cut(s) 1623, 2179
BstYI RGATCY 3 cut(s) 1698, 2129, 2206
BsuI GTATCC 1 cut(s) 1935
BtgI CCRYGG 3 cut(s) 130, 372, 1239
BtgZI GCGATG 1 cut(s) 103
BtrI CACGTC 1 cut(s) 839
BtsCI GGATG 8 cut(s) 201, 382, 1192, 1414, 2188, 2371, 2497, 2568
BtsI GCAGTG 3 cut(s) 162, 549, 876
BtsIMutI CAGTG 7 cut(s) 63, 162, 549, 634, 734, 876, 1392
Cac8I GCNNGC 7 cut(s) 86, 276, 634, 908, 1257, 1299, 1583
CaiI CAGNNNCTG 1 cut(s) 1582
CciI TCATGA 1 cut(s) 2350
CfoI GCGC 4 cut(s) 79, 161, 182, 1788
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 9 cut(s) 83, 134, 304, 356, 440, 446, 1579, 2270, 2291
CseI GACGC 4 cut(s) 83, 680, 962, 2053
Csp6I GTAC 4 cut(s) 22, 42, 1133, 1834
CspCI CAANNNNNGTGG 8 cut(s) 151, 186, 1441, 1476, 2407, 2439, 2442, 2474
CviQI GTAC 4 cut(s) 22, 42, 1133, 1834
DinI GGCGCC 2 cut(s) 160, 181
DraIII CACNNNGTG 1 cut(s) 421
EaeI YGGCCR 3 cut(s) 457, 2412, 2609
Eam1104I CTCTTC 2 cut(s) 155, 661
EarI CTCTTC 2 cut(s) 155, 661
EciI GGCGGA 2 cut(s) 181, 678
Ecl136II GAGCTC 1 cut(s) 2348
Eco130I CCWWGG 4 cut(s) 296, 1239, 1437, 1616
Eco147I AGGCCT 1 cut(s) 601
Eco24I GRGCYC 3 cut(s) 544, 587, 2350
Eco31I GGTCTC 3 cut(s) 44, 1034, 2255
Eco32I GATATC 4 cut(s) 488, 773, 1042, 1972
Eco47I GGWCC 2 cut(s) 356, 440
Eco53kI GAGCTC 1 cut(s) 2348
Eco57I CTGAAG 4 cut(s) 1110, 1290, 1350, 1709
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 2348
EcoO109I RGGNCCY 3 cut(s) 440, 1579, 2270
EcoRII CCWGG 7 cut(s) 14, 442, 536, 1059, 1559, 2272, 2385
EcoRV GATATC 4 cut(s) 488, 773, 1042, 1972
EcoT14I CCWWGG 4 cut(s) 296, 1239, 1437, 1616
EcoT22I ATGCAT 3 cut(s) 717, 2006, 2115
EcoT38I GRGCYC 3 cut(s) 544, 587, 2350
EgeI GGCGCC 2 cut(s) 160, 181
EheI GGCGCC 2 cut(s) 160, 181
ErhI CCWWGG 4 cut(s) 296, 1239, 1437, 1616
Esp3I CGTCTC 2 cut(s) 297, 663
FalI AAGNNNNNCTT 2 cut(s) 658, 690
FaqI GGGAC 6 cut(s) 74, 742, 951, 1294, 1555, 2403
FauI CCCGC 5 cut(s) 93, 205, 497, 641, 899
FbaI TGATCA 1 cut(s) 2397
FokI GGATG 8 cut(s) 188, 389, 1199, 1401, 2195, 2378, 2484, 2575
FriOI GRGCYC 3 cut(s) 544, 587, 2350
FspBI CTAG 6 cut(s) 264, 297, 609, 1212, 1284, 1907
GlaI GCGC 4 cut(s) 78, 160, 181, 1787
GsaI CCCAGC 2 cut(s) 366, 2190
GsuI CTGGAG 2 cut(s) 837, 906
HaeII RGCGCY 2 cut(s) 162, 183
HapII CCGG 9 cut(s) 26, 81, 198, 205, 275, 359, 750, 1634, 2525
HgaI GACGC 4 cut(s) 83, 680, 962, 2053
HhaI GCGC 4 cut(s) 79, 161, 182, 1788
Hin1I GRCGYC 2 cut(s) 159, 180
Hin6I GCGC 4 cut(s) 77, 159, 180, 1786
HinP1I GCGC 4 cut(s) 77, 159, 180, 1786
HindIII AAGCTT 1 cut(s) 2159
HinfI GANTC 7 cut(s) 267, 533, 739, 1193, 1516, 1658, 2515
HpaII CCGG 9 cut(s) 26, 81, 198, 205, 275, 359, 750, 1634, 2525
HphI GGTGA 9 cut(s) 97, 241, 293, 756, 863, 1167, 1202, 1787, 2514
Hpy166II GTNNAC 4 cut(s) 261, 738, 1557, 1939
Hpy188III TCNNGA 8 cut(s) 47, 49, 545, 1328, 1928, 2351, 2537, 2624
Hpy8I GTNNAC 4 cut(s) 261, 738, 1557, 1939
Hpy99I CGWCG 4 cut(s) 74, 77, 116, 978
HpyAV CCTTC 7 cut(s) 167, 584, 612, 771, 842, 848, 1421
HpyCH4III ACNGT 7 cut(s) 41, 291, 499, 1476, 1720, 1770, 2379
HpyCH4IV ACGT 4 cut(s) 838, 1832, 1948, 2542
HpySE526I ACGT 4 cut(s) 838, 1832, 1948, 2542
Hsp92I GRCGYC 2 cut(s) 159, 180
HspAI GCGC 4 cut(s) 77, 159, 180, 1786
KasI GGCGCC 2 cut(s) 158, 179
KroI GCCGGC 1 cut(s) 274
KroNI GCCGGC 1 cut(s) 276
Ksp22I TGATCA 1 cut(s) 2397
LmnI GCTCC 6 cut(s) 34, 857, 964, 1711, 1741, 2569
Lsp1109I GCAGC 9 cut(s) 304, 326, 769, 1001, 1252, 1292, 1675, 1951, 2068
LweI GCATC 6 cut(s) 80, 210, 685, 1004, 2173, 2553
MaeI CTAG 6 cut(s) 264, 297, 609, 1212, 1284, 1907
MaeII ACGT 4 cut(s) 838, 1832, 1948, 2542
MaeIII GTNAC 8 cut(s) 95, 217, 493, 811, 913, 1388, 2281, 2520
MbiI CCGCTC 1 cut(s) 636
MflI RGATCY 3 cut(s) 1698, 2129, 2206
MhlI GDGCHC 7 cut(s) 369, 544, 587, 634, 685, 826, 2350
MlsI TGGCCA 1 cut(s) 459
MluNI TGGCCA 1 cut(s) 459
Mly113I GGCGCC 2 cut(s) 159, 180
MlyI GAGTC 1 cut(s) 733
Mox20I TGGCCA 1 cut(s) 459
Mph1103I ATGCAT 3 cut(s) 717, 2006, 2115
MroNI GCCGGC 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 1649
MscI TGGCCA 1 cut(s) 459
MseI TTAA 5 cut(s) 1095, 1743, 1781, 1943, 2201
MslI CAYNNNNRTG 6 cut(s) 332, 720, 2108, 2177, 2355, 2561
Msp20I TGGCCA 1 cut(s) 459
MspA1I CMGCKG 4 cut(s) 317, 504, 884, 1729
MspI CCGG 9 cut(s) 26, 81, 198, 205, 275, 359, 750, 1634, 2525
Mva1269I GAATGC 1 cut(s) 2006
MvaI CCWGG 7 cut(s) 16, 444, 538, 1061, 1561, 2274, 2387
MvnI CGCG 4 cut(s) 77, 88, 311, 2047
NaeI GCCGGC 1 cut(s) 276
NarI GGCGCC 2 cut(s) 159, 180
NciI CCSGG 4 cut(s) 26, 81, 198, 750
NcoI CCATGG 1 cut(s) 1239
NgoMIV GCCGGC 1 cut(s) 274
NlaIV GGNNCC 5 cut(s) 30, 136, 160, 181, 684
NmuCI GTSAC 6 cut(s) 95, 217, 913, 1388, 2281, 2520
NsiI ATGCAT 3 cut(s) 717, 2006, 2115
NspI RCATGY 3 cut(s) 649, 782, 812
NspV TTCGAA 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 332
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 1 cut(s) 2350
PasI CCCWGGG 1 cut(s) 2386
PceI AGGCCT 1 cut(s) 601
PciI ACATGT 1 cut(s) 808
PcsI WCGNNNNNNNCGW 1 cut(s) 1662
PctI GAATGC 1 cut(s) 2006
PdiI GCCGGC 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 1649
PfeI GAWTC 6 cut(s) 267, 533, 1193, 1516, 1658, 2515
PflMI CCANNNNNTGG 2 cut(s) 258, 466
PleI GAGTC 1 cut(s) 733
PluTI GGCGCC 2 cut(s) 162, 183
PpsI GAGTC 1 cut(s) 733
Ppu21I YACGTR 1 cut(s) 1949
PpuMI RGGWCCY 1 cut(s) 440
PscI ACATGT 1 cut(s) 808
PshAI GACNNNNGTC 1 cut(s) 1477
PsiI TTATAA 1 cut(s) 2342
Psp124BI GAGCTC 1 cut(s) 2350
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 7 cut(s) 14, 442, 536, 1059, 1559, 2272, 2385
PspFI CCCAGC 2 cut(s) 362, 2186
PspGI CCWGG 7 cut(s) 14, 442, 536, 1059, 1559, 2272, 2385
PspN4I GGNNCC 5 cut(s) 30, 136, 160, 181, 684
PspPI GGNCC 9 cut(s) 83, 134, 304, 356, 440, 446, 1579, 2270, 2291
PspPPI RGGWCCY 1 cut(s) 440
PstI CTGCAG 1 cut(s) 322
PstNI CAGNNNCTG 1 cut(s) 1582
PsuI RGATCY 3 cut(s) 1698, 2129, 2206
PvuII CAGCTG 2 cut(s) 317, 1729
RsaI GTAC 4 cut(s) 23, 43, 1134, 1835
RsaNI GTAC 4 cut(s) 22, 42, 1133, 1834
RseI CAYNNNNRTG 6 cut(s) 332, 720, 2108, 2177, 2355, 2561
SacI GAGCTC 1 cut(s) 2350
SaqAI TTAA 5 cut(s) 1095, 1743, 1781, 1943, 2201
Sau96I GGNCC 9 cut(s) 83, 134, 304, 356, 440, 446, 1579, 2270, 2291
SchI GAGTC 1 cut(s) 733
SduI GDGCHC 7 cut(s) 369, 544, 587, 634, 685, 826, 2350
SfaNI GCATC 6 cut(s) 80, 210, 685, 1004, 2173, 2553
SfcI CTRYAG 4 cut(s) 318, 843, 1446, 2226
SfoI GGCGCC 2 cut(s) 160, 181
Sfr274I CTCGAG 1 cut(s) 47
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 2 cut(s) 356, 440
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 6 cut(s) 332, 720, 2108, 2177, 2355, 2561
SmlI CTYRAG 3 cut(s) 47, 1031, 1219
SmoI CTYRAG 3 cut(s) 47, 1031, 1219
SseBI AGGCCT 1 cut(s) 601
SspDI GGCGCC 2 cut(s) 158, 179
SspMI CTAG 6 cut(s) 264, 297, 609, 1212, 1284, 1907
SstI GAGCTC 1 cut(s) 2350
StuI AGGCCT 1 cut(s) 601
StyI CCWWGG 4 cut(s) 296, 1239, 1437, 1616
TaaI ACNGT 7 cut(s) 41, 291, 499, 1476, 1720, 1770, 2379
TaiI ACGT 4 cut(s) 841, 1835, 1951, 2545
TaqI TCGA 4 cut(s) 8, 48, 1656, 1870
TauI GCSGC 9 cut(s) 119, 180, 185, 305, 314, 351, 884, 995, 1820
TfiI GAWTC 6 cut(s) 267, 533, 1193, 1516, 1658, 2515
Tru1I TTAA 5 cut(s) 1095, 1743, 1781, 1943, 2201
Tru9I TTAA 5 cut(s) 1095, 1743, 1781, 1943, 2201
TscAI CASTG 7 cut(s) 70, 169, 556, 634, 734, 883, 1399
TseFI GTSAC 6 cut(s) 95, 217, 913, 1388, 2281, 2520
TseI GCWGC 9 cut(s) 314, 317, 782, 989, 1265, 1280, 1688, 1964, 2081
Tsp45I GTSAC 6 cut(s) 95, 217, 913, 1388, 2281, 2520
TspDTI ATGAA 9 cut(s) 221, 1068, 1136, 1403, 1538, 1751, 1812, 2367, 2486
TspGWI ACGGA 2 cut(s) 389, 940
TspRI CASTG 7 cut(s) 70, 169, 556, 634, 734, 883, 1399
Van91I CCANNNNNTGG 2 cut(s) 258, 466
VpaK11BI GGWCC 2 cut(s) 356, 440
XapI RAATTY 3 cut(s) 1072, 1234, 2137
XceI RCATGY 3 cut(s) 649, 782, 812
XhoI CTCGAG 1 cut(s) 47
XmaJI CCTAGG 1 cut(s) 296
XmnI GAANNNNTTC 1 cut(s) 1649
XspI CTAG 6 cut(s) 264, 297, 609, 1212, 1284, 1907
Zsp2I ATGCAT 3 cut(s) 717, 2006, 2115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.