Rh6BG345800

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0006473
Physical Location & Seq
Forward (+)
57735123 .. 57742294
7172 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG345800.1

Sequence Viewer

Length: 2316 bp
ATGAGTTCGAAACCATGGCTGTACCCGGCTCCTACCTATCGTACTCTCGAGACCTATTGGGATACCGACGACGACGCGCCCGGCGCGCGATGCGGTCACACGCTCACCGCCGTCGCAGCCACCAAGACCCACGGCCCCCGCCTTATTCTCTTCGGTGGCGCCACCGCCATTGAAGGCGGCGCAGCCTCCTCCGCCCCCGGCATCAGGCTAGCGGGTGTGACCAATTCTGTTCACTCCTACGATGTTCTCACCAGAAAGTGGACTAGAATCAGGCCGGCTGGTGAGCCACCCTCTCCGAGGGCGGCGCACGCTGCAGCTGCAGTGGGGACTATGGTGGTTTTTCAGGGTGGCATTGGTCCTGCTGGGCACTCCACGGATGATCTCTATGTGCTTGACATGGATAAGTTCAAGTGGCACAGAGTGGTTGTGCAAGGACAAGGACCTGGGCCTCGCTATGGCCATGTTATGGACTTAGTTGCTCAAAGATATCTTGTTACTGTCAGCGGCAATGATGGAAAAAGAGTTCTTTCTGATTCCTGGGCTCTGGATACTGCACAGAAACCTTACGTATGGGCTAAGCTGAGTCCAGAAGGGGATAGGCCTTCTGCTAGAATGTATGCAACAGCCAGTGCCCGCTCAGATGGCATGTTTTTGCTTTGTGGCGGAAGAGACGCTTCCGGGGCGCCTCTAGCTGATGCTTATGGACTGCTCATGCATAGAAATGGTCAGTGGGAGTGGACTCTCGCACCGGGAGTGTCTCCTTCACCAAGATATCAACATGCTGCGGTTTTTGTTGGTGCAAGATTACATGTAACGGGAGGTGTTCTCAGGGGAGGACGTGCTGTAGAAGGGGAAGGAGCTATTGCAGTATTGGACACTGCCGCTGGAGTTTGGTTAGATAGAAGCGGGCTTGTGACCTCCTCACGGACAGGCAAGGGACAAAATGACTATGATCCCCCTTTGGAGCTTATGCGTCGTTGTCGCCATGCAGCCGCATCTCTTGGTGTTCGTATTTACATTTATGGCGGTCTCAAGGGAGACATCCTGTTGGATGATTTCCTGGTTGCGGAAAATTCATCATTTCAATCTGAAATTAATTCTCCCGTATCAACTTCTGAGAAATCCCCATCTTTAACAAGTCCGAGAGCCTTTCATGCCAACACAAGTTCTTTTGGTGTATCACCATCTTCGGATGGTGAATCTGAGATACCCTCATCTAGTGGCTTGAGCATGGATAAAAATTCCATGGAGAAACTACGGCAGGCTTCTGCTGCCGAAGCTGAGGCAGCTAGTGCTGTTTGGCAAGCTGTGCAGTCCCAATCTTCCCCTCCTCCTGAAGAAACTTCTGTTTCAGATGACAACTCACAAATTGCAGAAACACTTTCGGATGGTAGTGACACTGAGGCAGATGTTCGCCTTCATCCCAGAGCTGTTGTTGTTGCCAAAGAGGCTGTAGGCAATTTGGGTGGCATGGTAAGACAGTTGTCTTTGGATCAATTCGAAAATGAGAGCAGACGAATGATTCCAATGAATAACGACTTATCATATCCTAACAAAAAGTTTACCAGGCAGAAGTCCCCACAGGGCTTACATAAGAAGATAATATCTACATTGCTGAGGCCAAGGAACTGGAAACCTCCGGTTAATAGGAAGTTTTTCCTCGATTCTTACGAAGTGGGAGAACTTTGTTATGCTGCTGAACAGATCTTTATGCAGGAGCAAACAGTTCTTCAGCTGAAAGCTCCTATTAAAGTATTTGGCGATCTTCATGGACAGTTTGGTGATTTAATGCGCTTGTTTGATGAATATGGATTTCCATCTGCTGCAGGAGACATAACGTACATTGACTACTTGTTTTTGGGGGATTATGTTGATCGAGGACAGCACAGCTTGGAGACAATAACTCTACTCCTTGCACTCAAGATTGAGTATCCTGATAATGTTCACTTAATACGTGGAAACCATGAGGCTGCTGATATCAACGCACTCTTTGGTTTTCGTATTGAATGCATTGAGAGAATGGGAGAGAGTGATGGAATATGGGCATGGACAAGGTTCAATCAACTTTTCAACTACCTCCCACTGGCTGCACTAATTGAAAAGAAAATCATCTGTATGCATGGTGGCATTGGAAGATCTATAAATTCTGTAGAACAAATAGAGAAGCTTGAAAGACCCATTACAATGGATGCTGGGTCTATAATTTTAATGGATCTTCTATGGTCTGATCCTACAGAAAACGATGGTGTAGAGGGTCTCAGACCAAATGCAAGAGGGCCTGGTCTTGTCACTTTTGGGGTAAGCACAATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4 Metallophos STPPase_N

Protein Analysis

771

Amino Acids

83.83

Weight (kDa)

5.86

Isoelectric Point (pI)

39.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 21 - 218 1.1e-32 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 22 - 186 4e-25 FBX42, beta-propeller domain
Kelch_HCF PF13854 72 - 346 8.3e-16 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 174 - 355 4.9e-12 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 201 - 276 7.4e-07 tRNA wybutosine-synthesizing protein 4, beta-propeller
Metallophos PF00149 582 - 757 2.3e-32 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 158, 682
AccB7I CCANNNNNTGG 2 cut(s) 258, 466
AccBSI CCGCTC 1 cut(s) 636
AccII CGCG 3 cut(s) 77, 86, 88
AclWI GGATC 4 cut(s) 947, 1500, 2220, 2222
AcoI YGGCCR 1 cut(s) 457
AcsI RAATTY 3 cut(s) 1072, 1240, 2143
AcuI CTGAAG 2 cut(s) 1356, 1715
AcyI GRCGYC 2 cut(s) 159, 683
AdeI CACNNNGTG 1 cut(s) 421
AfaI GTAC 3 cut(s) 23, 43, 1841
AfiI CCNNNNNNNGG 8 cut(s) 204, 258, 297, 455, 466, 924, 1066, 2083
AflIII ACRYGT 1 cut(s) 808
AgsI TTSAA 8 cut(s) 173, 409, 1085, 2006, 2059, 2071, 2099, 2171
AjiI CACGTC 1 cut(s) 839
AjnI CCWGG 5 cut(s) 442, 536, 1059, 1565, 2278
AloI GAACNNNNNNTCC 2 cut(s) 507, 539
Alw26I GTCTC 8 cut(s) 44, 663, 762, 1032, 1034, 1824, 1889, 2261
AlwI GGATC 4 cut(s) 947, 1500, 2220, 2222
Ama87I CYCGRG 1 cut(s) 47
AoxI GGCC 7 cut(s) 133, 272, 446, 457, 599, 1619, 2276
ApoI RAATTY 3 cut(s) 1072, 1240, 2143
AseI ATTAAT 1 cut(s) 1095
Asp700I GAANNNNTTC 1 cut(s) 1655
AspLEI GCGC 8 cut(s) 79, 86, 88, 161, 182, 307, 685, 1794
AspS9I GGNCC 5 cut(s) 134, 356, 440, 446, 2276
AsuC2I CCSGG 5 cut(s) 26, 81, 198, 679, 750
AsuHPI GGTGA 7 cut(s) 97, 241, 293, 756, 1173, 1208, 1793
AsuII TTCGAA 2 cut(s) 8, 1500
AsuNHI GCTAGC 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 2 cut(s) 356, 440
BaeGI GKGCMC 2 cut(s) 369, 634
BalI TGGCCA 1 cut(s) 459
BanI GGYRCC 2 cut(s) 158, 682
BanII GRGCYC 1 cut(s) 544
BbvCI CCTCAGC 2 cut(s) 1281, 1616
BccI CCATC 9 cut(s) 506, 635, 1135, 1187, 1192, 1382, 1825, 2027, 2237
BceAI ACGGC 3 cut(s) 95, 148, 1274
BciT130I CCWGG 5 cut(s) 444, 538, 1061, 1567, 2280
BciVI GTATCC 3 cut(s) 55, 541, 1941
BcnI CCSGG 5 cut(s) 26, 81, 198, 679, 750
BcoDI GTCTC 8 cut(s) 44, 663, 762, 1032, 1034, 1824, 1889, 2261
BfaI CTAG 6 cut(s) 209, 264, 609, 689, 1218, 1290
BfmI CTRYAG 7 cut(s) 312, 318, 843, 1452, 1824, 2148, 2232
BfoI RGCGCY 2 cut(s) 162, 686
BfuI GTATCC 3 cut(s) 55, 541, 1941
BglI GCCNNNNNGGC 1 cut(s) 1448
BglII AGATCT 2 cut(s) 1704, 2135
BlpI GCTNAGC 1 cut(s) 576
Bme18I GGWCC 2 cut(s) 356, 440
BmeT110I CYCGRG 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 839
BmgT120I GGNCC 5 cut(s) 134, 356, 440, 446, 2276
BmiI GGNNCC 4 cut(s) 30, 136, 160, 684
BmsI GCATC 5 cut(s) 80, 210, 685, 1004, 2179
BmtI GCTAGC 1 cut(s) 212
BoxI GACNNNNGTC 1 cut(s) 1483
BplI GAGNNNNNCTC 6 cut(s) 275, 307, 810, 842, 1196, 1228
BpmI CTGGAG 1 cut(s) 906
Bpu10I CCTNAGC 2 cut(s) 1281, 1616
Bpu1102I GCTNAGC 1 cut(s) 576
Bpu14I TTCGAA 2 cut(s) 8, 1500
BpuEI CTTGAG 3 cut(s) 1016, 1246, 1904
BpuMI CCSGG 5 cut(s) 26, 81, 198, 679, 750
BsaAI YACGTR 2 cut(s) 568, 1955
BsaBI GATNNNNATC 1 cut(s) 1816
BsaHI GRCGYC 2 cut(s) 159, 683
BsaI GGTCTC 3 cut(s) 44, 1034, 2261
BsaWI WCCGGW 1 cut(s) 1639
Bsc4I CCNNNNNNNGG 8 cut(s) 204, 258, 297, 455, 466, 924, 1066, 2083
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 3 cut(s) 627, 1634, 2088
Bse3DI GCAATG 2 cut(s) 514, 1610
Bse8I GATNNNNATC 1 cut(s) 1816
BseBI CCWGG 5 cut(s) 444, 538, 1061, 1567, 2280
BseGI GGATG 7 cut(s) 382, 1041, 1057, 1198, 1393, 1420, 2194
BseJI GATNNNNATC 1 cut(s) 1816
BseLI CCNNNNNNNGG 8 cut(s) 204, 258, 297, 455, 466, 924, 1066, 2083
BseMI GCAATG 2 cut(s) 514, 1610
BseMII CTCAG 9 cut(s) 572, 651, 841, 1107, 1194, 1272, 1392, 1607, 2272
BseNI ACTGG 3 cut(s) 627, 1634, 2088
BsePI GCGCGC 1 cut(s) 84
BseRI GAGGAG 3 cut(s) 178, 910, 1320
BseSI GKGCMC 2 cut(s) 369, 634
BseYI CCCAGC 2 cut(s) 362, 2192
BsgI GTGCAG 3 cut(s) 537, 1331, 2073
Bsh1236I CGCG 3 cut(s) 77, 86, 88
BshFI GGCC 7 cut(s) 135, 274, 448, 459, 601, 1621, 2278
BshNI GGYRCC 2 cut(s) 158, 682
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 7 cut(s) 26, 81, 198, 275, 678, 749, 1640
BslFI GGGAC 4 cut(s) 340, 951, 1300, 1561
BslI CCNNNNNNNGG 8 cut(s) 204, 258, 297, 455, 466, 924, 1066, 2083
BsmAI GTCTC 8 cut(s) 44, 663, 762, 1032, 1034, 1824, 1889, 2261
BsmBI CGTCTC 1 cut(s) 663
BsmFI GGGAC 4 cut(s) 340, 951, 1300, 1561
BsmI GAATGC 1 cut(s) 2012
BsnI GGCC 7 cut(s) 135, 274, 448, 459, 601, 1621, 2278
Bso31I GGTCTC 3 cut(s) 44, 1034, 2261
BsoBI CYCGRG 1 cut(s) 47
Bsp119I TTCGAA 2 cut(s) 8, 1500
Bsp1286I GDGCHC 3 cut(s) 369, 544, 634
Bsp143I GATC 9 cut(s) 379, 952, 1492, 1704, 1762, 1873, 2135, 2212, 2227
Bsp1720I GCTNAGC 1 cut(s) 576
Bsp19I CCATGG 2 cut(s) 14, 1245
BspANI GGCC 7 cut(s) 135, 274, 448, 459, 601, 1621, 2278
BspCNI CTCAG 9 cut(s) 573, 650, 840, 1108, 1195, 1273, 1393, 1608, 2271
BspFNI CGCG 3 cut(s) 77, 86, 88
BspLI GGNNCC 4 cut(s) 30, 136, 160, 684
BspMAI CTGCAG 3 cut(s) 316, 322, 1828
BspOI GCTAGC 1 cut(s) 212
BspPI GGATC 4 cut(s) 947, 1500, 2220, 2222
BspT104I TTCGAA 2 cut(s) 8, 1500
BspT107I GGYRCC 2 cut(s) 158, 682
BspTNI GGTCTC 3 cut(s) 44, 1034, 2261
BsrBI CCGCTC 1 cut(s) 636
BsrDI GCAATG 2 cut(s) 514, 1610
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 3 cut(s) 627, 1634, 2088
BssAI RCCGGY 1 cut(s) 274
BssHII GCGCGC 1 cut(s) 84
BssMI GATC 9 cut(s) 379, 952, 1492, 1704, 1762, 1873, 2135, 2212, 2227
BssNI GRCGYC 2 cut(s) 159, 683
BssT1I CCWWGG 3 cut(s) 14, 1245, 1622
Bst2UI CCWGG 5 cut(s) 444, 538, 1061, 1567, 2280
Bst4CI ACNGT 4 cut(s) 499, 1482, 1726, 1776
Bst6I CTCTTC 2 cut(s) 155, 661
BstACI GRCGYC 2 cut(s) 159, 683
BstAPI GCANNNNNTGC 2 cut(s) 1292, 1309
BstBAI YACGTR 2 cut(s) 568, 1955
BstBI TTCGAA 2 cut(s) 8, 1500
BstC8I GCNNGC 8 cut(s) 86, 210, 276, 309, 634, 908, 1263, 1305
BstDSI CCRYGG 4 cut(s) 14, 130, 372, 1245
BstENI CCTNNNNNAGG 1 cut(s) 295
BstF5I GGATG 7 cut(s) 382, 1041, 1057, 1198, 1393, 1420, 2194
BstFNI CGCG 3 cut(s) 77, 86, 88
BstH2I RGCGCY 2 cut(s) 162, 686
BstHHI GCGC 8 cut(s) 79, 86, 88, 161, 182, 307, 685, 1794
BstKTI GATC 9 cut(s) 382, 955, 1495, 1707, 1765, 1876, 2138, 2215, 2230
BstMAI GTCTC 8 cut(s) 44, 663, 762, 1032, 1034, 1824, 1889, 2261
BstMBI GATC 9 cut(s) 379, 952, 1492, 1704, 1762, 1873, 2135, 2212, 2227
BstNI CCWGG 5 cut(s) 444, 538, 1061, 1567, 2280
BstNSI RCATGY 3 cut(s) 649, 782, 812
BstPAI GACNNNNGTC 1 cut(s) 1483
BstSFI CTRYAG 7 cut(s) 312, 318, 843, 1452, 1824, 2148, 2232
BstSLI GKGCMC 2 cut(s) 369, 634
BstSNI TACGTA 1 cut(s) 568
BstUI CGCG 3 cut(s) 77, 86, 88
BstX2I RGATCY 3 cut(s) 1704, 2135, 2212
BstXI CCANNNNNNTGG 2 cut(s) 1629, 2185
BstYI RGATCY 3 cut(s) 1704, 2135, 2212
BsuI GTATCC 3 cut(s) 55, 541, 1941
BsuRI GGCC 7 cut(s) 135, 274, 448, 459, 601, 1621, 2278
BtgI CCRYGG 4 cut(s) 14, 130, 372, 1245
BtgZI GCGATG 1 cut(s) 103
BtrI CACGTC 1 cut(s) 839
BtsCI GGATG 7 cut(s) 382, 1041, 1057, 1198, 1393, 1420, 2194
BtsI GCAGTG 2 cut(s) 327, 876
BtsIMutI CAGTG 6 cut(s) 327, 634, 734, 876, 1398, 2081
Cac8I GCNNGC 8 cut(s) 86, 210, 276, 309, 634, 908, 1263, 1305
CfoI GCGC 8 cut(s) 79, 86, 88, 161, 182, 307, 685, 1794
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 5 cut(s) 134, 356, 440, 446, 2276
CseI GACGC 3 cut(s) 83, 680, 962
Csp6I GTAC 3 cut(s) 22, 42, 1840
CspCI CAANNNNNGTGG 4 cut(s) 151, 186, 1447, 1482
CviQI GTAC 3 cut(s) 22, 42, 1840
DinI GGCGCC 2 cut(s) 160, 684
DpnI GATC 9 cut(s) 381, 954, 1494, 1706, 1764, 1875, 2137, 2214, 2229
DpnII GATC 9 cut(s) 379, 952, 1492, 1704, 1762, 1873, 2135, 2212, 2227
DraIII CACNNNGTG 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 457
Eam1104I CTCTTC 2 cut(s) 155, 661
EarI CTCTTC 2 cut(s) 155, 661
EciI GGCGGA 2 cut(s) 181, 678
Eco105I TACGTA 1 cut(s) 568
Eco130I CCWWGG 3 cut(s) 14, 1245, 1622
Eco147I AGGCCT 1 cut(s) 601
Eco24I GRGCYC 1 cut(s) 544
Eco31I GGTCTC 3 cut(s) 44, 1034, 2261
Eco32I GATATC 3 cut(s) 488, 773, 1978
Eco47I GGWCC 2 cut(s) 356, 440
Eco57I CTGAAG 2 cut(s) 1356, 1715
Eco88I CYCGRG 1 cut(s) 47
EcoNI CCTNNNNNAGG 1 cut(s) 295
EcoO109I RGGNCCY 2 cut(s) 440, 2276
EcoRII CCWGG 5 cut(s) 442, 536, 1059, 1565, 2278
EcoRV GATATC 3 cut(s) 488, 773, 1978
EcoT14I CCWWGG 3 cut(s) 14, 1245, 1622
EcoT22I ATGCAT 3 cut(s) 717, 2012, 2121
EcoT38I GRGCYC 1 cut(s) 544
EgeI GGCGCC 2 cut(s) 160, 684
EheI GGCGCC 2 cut(s) 160, 684
ErhI CCWWGG 3 cut(s) 14, 1245, 1622
Esp3I CGTCTC 1 cut(s) 663
FalI AAGNNNNNCTT 2 cut(s) 658, 690
FaqI GGGAC 4 cut(s) 340, 951, 1300, 1561
FauI CCCGC 4 cut(s) 146, 205, 641, 899
FokI GGATG 7 cut(s) 389, 1028, 1064, 1205, 1400, 1407, 2201
FriOI GRGCYC 1 cut(s) 544
FspBI CTAG 6 cut(s) 209, 264, 609, 689, 1218, 1290
GlaI GCGC 8 cut(s) 78, 85, 87, 160, 181, 306, 684, 1793
GsaI CCCAGC 2 cut(s) 366, 2196
GsuI CTGGAG 1 cut(s) 906
HaeII RGCGCY 2 cut(s) 162, 686
HaeIII GGCC 7 cut(s) 135, 274, 448, 459, 601, 1621, 2278
HapII CCGG 7 cut(s) 26, 81, 198, 275, 678, 749, 1640
HgaI GACGC 3 cut(s) 83, 680, 962
HhaI GCGC 8 cut(s) 79, 86, 88, 161, 182, 307, 685, 1794
Hin1I GRCGYC 2 cut(s) 159, 683
Hin6I GCGC 8 cut(s) 77, 84, 86, 159, 180, 305, 683, 1792
HinP1I GCGC 8 cut(s) 77, 84, 86, 159, 180, 305, 683, 1792
HindIII AAGCTT 1 cut(s) 2165
HinfI GANTC 7 cut(s) 267, 533, 583, 739, 1199, 1522, 1664
HpaII CCGG 7 cut(s) 26, 81, 198, 275, 678, 749, 1640
HphI GGTGA 7 cut(s) 97, 241, 293, 756, 1173, 1208, 1793
Hpy166II GTNNAC 5 cut(s) 232, 261, 738, 1563, 1945
Hpy188III TCNNGA 7 cut(s) 47, 49, 545, 587, 1334, 1921, 1934
Hpy8I GTNNAC 5 cut(s) 232, 261, 738, 1563, 1945
Hpy99I CGWCG 5 cut(s) 71, 74, 77, 116, 978
HpyAV CCTTC 7 cut(s) 167, 584, 612, 771, 842, 848, 1427
HpyCH4III ACNGT 4 cut(s) 499, 1482, 1726, 1776
HpyCH4IV ACGT 4 cut(s) 567, 838, 1838, 1954
HpySE526I ACGT 4 cut(s) 567, 838, 1838, 1954
Hsp92I GRCGYC 2 cut(s) 159, 683
HspAI GCGC 8 cut(s) 77, 84, 86, 159, 180, 305, 683, 1792
KasI GGCGCC 2 cut(s) 158, 682
KroI GCCGGC 1 cut(s) 274
KroNI GCCGGC 1 cut(s) 276
Kzo9I GATC 9 cut(s) 379, 952, 1492, 1704, 1762, 1873, 2135, 2212, 2227
LmnI GCTCC 5 cut(s) 34, 857, 964, 1717, 1747
LweI GCATC 5 cut(s) 80, 210, 685, 1004, 2179
MaeI CTAG 6 cut(s) 209, 264, 609, 689, 1218, 1290
MaeII ACGT 4 cut(s) 567, 838, 1838, 1954
MaeIII GTNAC 7 cut(s) 95, 217, 493, 811, 913, 1394, 2287
MalI GATC 9 cut(s) 381, 954, 1494, 1706, 1764, 1875, 2137, 2214, 2229
MbiI CCGCTC 1 cut(s) 636
MboI GATC 9 cut(s) 379, 952, 1492, 1704, 1762, 1873, 2135, 2212, 2227
MflI RGATCY 3 cut(s) 1704, 2135, 2212
MhlI GDGCHC 3 cut(s) 369, 544, 634
MlsI TGGCCA 1 cut(s) 459
MluNI TGGCCA 1 cut(s) 459
Mly113I GGCGCC 2 cut(s) 159, 683
MlyI GAGTC 2 cut(s) 592, 733
MmeI TCCRAC 1 cut(s) 1029
Mox20I TGGCCA 1 cut(s) 459
Mph1103I ATGCAT 3 cut(s) 717, 2012, 2121
MroNI GCCGGC 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 1655
MscI TGGCCA 1 cut(s) 459
MseI TTAA 7 cut(s) 1095, 1133, 1644, 1749, 1787, 1949, 2207
MslI CAYNNNNRTG 3 cut(s) 720, 2114, 2183
Msp20I TGGCCA 1 cut(s) 459
MspA1I CMGCKG 4 cut(s) 317, 504, 884, 1735
MspI CCGG 7 cut(s) 26, 81, 198, 275, 678, 749, 1640
Mva1269I GAATGC 1 cut(s) 2012
MvaI CCWGG 5 cut(s) 444, 538, 1061, 1567, 2280
MvnI CGCG 3 cut(s) 77, 86, 88
NaeI GCCGGC 1 cut(s) 276
NarI GGCGCC 2 cut(s) 159, 683
NciI CCSGG 5 cut(s) 26, 81, 198, 679, 750
NcoI CCATGG 2 cut(s) 14, 1245
NdeII GATC 9 cut(s) 379, 952, 1492, 1704, 1762, 1873, 2135, 2212, 2227
NgoMIV GCCGGC 1 cut(s) 274
NheI GCTAGC 1 cut(s) 208
NlaIV GGNNCC 4 cut(s) 30, 136, 160, 684
NmuCI GTSAC 5 cut(s) 95, 217, 913, 1394, 2287
NsiI ATGCAT 3 cut(s) 717, 2012, 2121
NspI RCATGY 3 cut(s) 649, 782, 812
NspV TTCGAA 2 cut(s) 8, 1500
PaeR7I CTCGAG 1 cut(s) 47
PauI GCGCGC 1 cut(s) 84
PceI AGGCCT 1 cut(s) 601
PciI ACATGT 1 cut(s) 808
PcsI WCGNNNNNNNCGW 1 cut(s) 1668
PctI GAATGC 1 cut(s) 2012
PdiI GCCGGC 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 1655
PfeI GAWTC 5 cut(s) 267, 533, 1199, 1522, 1664
PflMI CCANNNNNTGG 2 cut(s) 258, 466
PleI GAGTC 2 cut(s) 591, 733
PluTI GGCGCC 2 cut(s) 162, 686
PpsI GAGTC 2 cut(s) 591, 733
Ppu21I YACGTR 2 cut(s) 568, 1955
PpuMI RGGWCCY 1 cut(s) 440
PscI ACATGT 1 cut(s) 808
PshAI GACNNNNGTC 1 cut(s) 1483
PshBI ATTAAT 1 cut(s) 1095
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 5 cut(s) 442, 536, 1059, 1565, 2278
PspFI CCCAGC 2 cut(s) 362, 2192
PspGI CCWGG 5 cut(s) 442, 536, 1059, 1565, 2278
PspN4I GGNNCC 4 cut(s) 30, 136, 160, 684
PspPI GGNCC 5 cut(s) 134, 356, 440, 446, 2276
PspPPI RGGWCCY 1 cut(s) 440
PstI CTGCAG 3 cut(s) 316, 322, 1828
PsuI RGATCY 3 cut(s) 1704, 2135, 2212
PteI GCGCGC 1 cut(s) 84
PvuII CAGCTG 2 cut(s) 317, 1735
RsaI GTAC 3 cut(s) 23, 43, 1841
RsaNI GTAC 3 cut(s) 22, 42, 1840
RseI CAYNNNNRTG 3 cut(s) 720, 2114, 2183
SaqAI TTAA 7 cut(s) 1095, 1133, 1644, 1749, 1787, 1949, 2207
Sau3AI GATC 9 cut(s) 379, 952, 1492, 1704, 1762, 1873, 2135, 2212, 2227
Sau96I GGNCC 5 cut(s) 134, 356, 440, 446, 2276
SchI GAGTC 2 cut(s) 592, 733
SduI GDGCHC 3 cut(s) 369, 544, 634
SfaNI GCATC 5 cut(s) 80, 210, 685, 1004, 2179
SfcI CTRYAG 7 cut(s) 312, 318, 843, 1452, 1824, 2148, 2232
SfoI GGCGCC 2 cut(s) 160, 684
Sfr274I CTCGAG 1 cut(s) 47
SfuI TTCGAA 2 cut(s) 8, 1500
SinI GGWCC 2 cut(s) 356, 440
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 3 cut(s) 720, 2114, 2183
SmlI CTYRAG 4 cut(s) 47, 1031, 1225, 1919
SmoI CTYRAG 4 cut(s) 47, 1031, 1225, 1919
SnaBI TACGTA 1 cut(s) 568
SseBI AGGCCT 1 cut(s) 601
SspDI GGCGCC 2 cut(s) 158, 682
SspMI CTAG 6 cut(s) 209, 264, 609, 689, 1218, 1290
StuI AGGCCT 1 cut(s) 601
StyI CCWWGG 3 cut(s) 14, 1245, 1622
TaaI ACNGT 4 cut(s) 499, 1482, 1726, 1776
TaiI ACGT 4 cut(s) 570, 841, 1841, 1957
TaqI TCGA 5 cut(s) 8, 48, 1500, 1662, 1876
TauI GCSGC 5 cut(s) 180, 305, 507, 884, 995
TfiI GAWTC 5 cut(s) 267, 533, 1199, 1522, 1664
Tru1I TTAA 7 cut(s) 1095, 1133, 1644, 1749, 1787, 1949, 2207
Tru9I TTAA 7 cut(s) 1095, 1133, 1644, 1749, 1787, 1949, 2207
TscAI CASTG 6 cut(s) 327, 634, 734, 883, 1405, 2088
TseFI GTSAC 5 cut(s) 95, 217, 913, 1394, 2287
Tsp45I GTSAC 5 cut(s) 95, 217, 913, 1394, 2287
TspDTI ATGAA 6 cut(s) 1065, 1142, 1409, 1544, 1757, 1818
TspGWI ACGGA 2 cut(s) 389, 940
TspRI CASTG 6 cut(s) 327, 634, 734, 883, 1405, 2088
Van91I CCANNNNNTGG 2 cut(s) 258, 466
VpaK11BI GGWCC 2 cut(s) 356, 440
VspI ATTAAT 1 cut(s) 1095
XagI CCTNNNNNAGG 1 cut(s) 295
XapI RAATTY 3 cut(s) 1072, 1240, 2143
XceI RCATGY 3 cut(s) 649, 782, 812
XhoI CTCGAG 1 cut(s) 47
XmnI GAANNNNTTC 1 cut(s) 1655
XspI CTAG 6 cut(s) 209, 264, 609, 689, 1218, 1290
Zsp2I ATGCAT 3 cut(s) 717, 2012, 2121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.