Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0006473

Physical Location & Seq

Genomic Coordinates Forward (+)

28726353 .. 28731888

Gene Structure 5536 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom11g26080.12

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGGGGTCGAAGCCATGGCTGTTCCCGGCTCCGACCTATCGTACTCTCGAGACCTTTTGGGATTGCGACGACGACGCCCCTGGTCCACGCTGCGCTCACACCCTCACCGCCGTCGCCGCCACCAAAACCCACGGCCCCCGTCTCATCCTTTTCGGTGGTGCCACCGCCATTGAAGGCGGTGGCCCCTCCTCCGCCCCTGGCATCAGGTTAGCTGGGGTGACCAATTCGGTGCATTCCTATGATATTCTCACCAGGAAATGGACTAGAATGAGGCCTGCAGGTGAGCCACCTTCTCCGAGGGCAGCTCACGCGGCAGCTGCGGTGGGCACTATGGTTGTATTTCAGGGCGGCATAGGTCCTGCTGGGCATTCCACAGATGATCTCTACGTGCTGGACTTAACCAACGATAAATTTAAATGGCACAGAGTGGTTGTACAAGGACAAGGACCCGGGCCTCGCTACGGCCATGTAATGGACTTAGTTGCTCAAAGATATCTTGTTACCGTCAGCGGAAATGATGGAAAAAGAGTTCTTTCTGATGCTTGGGCTCTGGATACTGCTCAAAAACCTTATGCATGGGCTAGGCTGAACCCAGAAGGTGATAGACCATCTGCTAGAATGTATGCAACAGCCAGTGCTCGCTCAGATGGTATGTTTTTGCTTTGTGGTGGAAGAGATATTTCTGGAACGCCTCTAGCAGACGCTTATGGACTGCTTATGCATAGGAATGGTCAGTGGGAGTGGACCCTTGCACCTGGTGTATCCCCTTCACCAAGATATGAACATGCGGCGGTTTTTGTTGGCGCAAGATTACATGTTACAGGAGGTGCTCTTAGGGGAGGTCGTGGTATAGAAGGTGAAGCAGCTATTGCAGTATTGGACACTGCTGCTGGAGTTTGGTTAGACAGAAATGGGCTTGTGACTTCCTCACGCACAGGAAAGGGACAGAATGACCATGATCCTTCTTTGGAGCTCATGCGTCGTTTTCGCCACGCAGCTGCATCTCTTGGTTTTCGTATATATATTTATGGTGGTCTTAGGGGAGACATCCTGCTAGATGACTTCCTGGTTGCAGAAAATTCATCAGTTCAGTCTGAAGGTAATTTGCCTGTTTTAACATCTGAGAGATCCCCAACTGTATCCAGTCCCAGAATGTTTCATGCTAACACAAGTACTTTTGGAACATCTTCTGATGGTGAACCTGAGAGTACCTCATCAACTGGCTTGAGCATGGACAAAAATTCAATGGAGAAACTCCGGGAGGCTTCTGCTGCCGAAGCTGAGGCAGCCAGTGCTGTTTGGCAAGCTGCGCAATCAGCATCTGCTACTCCTTCTGAAGAAACATCTGTTTCAGATGATAACTCACAAGTTGCAGAAACATTGTCTGATGGTAGTGACACCGAGGCAGATGTTCGTCTTCATCACAGAGCTGTTGTCGTTGCCAAAGAGGCTGTAGGCAACTTGGGTGGGATGGTGAGACAGTTGTCTTTGGATCAATTTGAAAATGAGAGCAGACGAACGATTCCAATGAACAATGACTTATCACATCCTAACAAAAAGTACACCAGGCAGAAGTCTCCACAAGGGCTACATAAGAAGGTTATATCTACATTGTTGAGGCCTCGAAACTGGAAACCCCCTGTCAATAGGAAGTTTTTCCTGGATTCTTATGAAGTGGGAGAACTTTGTTATGCTGCTGAGCAGATATTTATGCAGGAGCAAACAGTTCTTCAGCTGAAAGCCCCTGTTAAAGTATTTGTCGATCTTCATGGACAGTTTGGTGATTTAATGCGCTTATTTGATGAATATGGATTTCCATCTGCTGCGGGAGACATAACGTATATCGACTACTTGTTTTTGGGGGATTATGTCGATCGAGGACAGCACAGCTTGGAGACCATAACTTTGCTCCTTGCACTGAAGATCGAGTATCCTGACAATGTTCACTTAATACGTGGAAACCATGAAGCTGCTGATATCAATGCACTCTTTGGTTTTCGTATTGAATGCGTTGAGAGAATGGGCGAGAATGATGGAATCTGGGCATGGACTCGGTTCAATCAACTTTTCAACTATCTCCCACTTGCTGCTCTGATTGAAAAGAAAATTATCTGTATGCATGGAGGCATTGGGAGATCTATCAATTCTGTAGAACAGATAGAGAAGCTTGAAAGACCCATTACAATGGATGCTGGCTCTATAATTTTAATGGATCTTTTGTGGTCTGATCCCACAGAAAACGACAGTGTAGAGGGCTTGAGACCAAATGCCAGAGGACCTGGTCTTGTCACCTTTGGGCCTGATCGTGTCACAGAATTTTGTAAGAGAAACAAATTACAACTCATTGTAAGAGCTCATGAATGTGTCATGGATGGATTTGAAAGATTTGCCCAGGGACAGTTGATCACTCTTTTTTCTGCAACCAACTATTGCGGGACGGCAAACAATGCTGGTGCTATACTGGTCGTTGGCAGAGGTTTGGTTGTGGTTCCAAAATTAATCCATCCTTTGCCACCTCCTCTTCAGTCACCGGAGACATCTCCTGAACGGGTCATAGACGACGCATGGATGCAGGAGCTCAACATTCAAAGACCACCAACGCCTACTCGTGGTCGACCGCAGCCTGATCTTGATCGAAACTCACTTGCATATATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kelch_3 Kelch_4 Metallophos STPPase_N

Protein Analysis

886

Amino Acids

96.74

Weight (kDa)

5.97

Isoelectric Point (pI)

36.7

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006473)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G03080
fragaria_vesca	FvH4_2g24460 FvH4_2g24460 FvH4_2g24460 FvH4_2g24460
malus_domestica	MD03G1274100.v1.1 MD11G1296400.v1.1
prunus_persica	Prupe.8G250300_v2.0.a1 Prupe.8G268100_v2.0.a1 Prupe.8G268200_v2.0.a1
pyrus_communis	pycom03g21840 pycom03g21850 pycom11g26080
rosa_chinensis	RchiOBHm_Chr6g0292301
rosa_laevigata	RLG00000012044
rosa_multiflora	Rmu_co8058994.1_g000001 Rmu_co8428867.1_g000001 Rmu_sc0002993.1_g000020 Rmu_sc0011960.1_g000001
rosa_roxburghii	Rroxscaffold_7G00175230
rosa_rugosa	Rorug06G0225300
rosa_samantha	Rh6AG338700 Rh6BG345800 Rh6CG352900 Rh6DG338700
rosa_wichuraiana	Rw6G029480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	269
Acc16I	TGCGCA	1 cut(s)	1311
Acc36I	ACCTGC	1 cut(s)	269
AccB1I	GGYRCC	1 cut(s)	158
AccB7I	CCANNNNNTGG	2 cut(s)	258, 472
AccI	GTMKAC	1 cut(s)	2614
AccII	CGCG	1 cut(s)	311
AclWI	GGATC	5 cut(s)	953, 1122, 1500, 2220, 2222
AcoI	YGGCCR	1 cut(s)	463
AcsI	RAATTY	4 cut(s)	410, 1078, 1240, 2315
AcuI	CTGAAG	5 cut(s)	1116, 1356, 1715, 1940, 2507
AcyI	GRCGYC	1 cut(s)	75
AdeI	CACNNNGTG	2 cut(s)	427, 758
AfaI	GTAC	5 cut(s)	43, 435, 1174, 1210, 1562
AfiI	CCNNNNNNNGG	4 cut(s)	258, 461, 472, 2609
AflIII	ACRYGT	1 cut(s)	814
AjnI	CCWGG	9 cut(s)	79, 196, 251, 754, 1065, 1565, 1659, 2278, 2391
AloI	GAACNNNNNNTCC	2 cut(s)	513, 545
Alw21I	GWGCWC	5 cut(s)	640, 832, 975, 2356, 2580
Alw26I	GTCTC	9 cut(s)	44, 146, 1038, 1471, 1581, 1824, 1889, 2254, 2528
AlwI	GGATC	5 cut(s)	953, 1122, 1500, 2220, 2222
AlwNI	CAGNNNCTG	1 cut(s)	1322
Ama87I	CYCGRG	2 cut(s)	47, 449
AoxI	GGCC	7 cut(s)	133, 181, 272, 452, 463, 1619, 2297
ApoI	RAATTY	4 cut(s)	410, 1078, 1240, 2315
ArsI	GACNNNNNNTTYG	2 cut(s)	1888, 1920
AseI	ATTAAT	1 cut(s)	2498
Asp700I	GAANNNNTTC	2 cut(s)	1186, 1655
AspLEI	GCGC	4 cut(s)	95, 806, 1312, 1794
AspS9I	GGNCC	9 cut(s)	83, 134, 182, 356, 446, 452, 744, 2276, 2297
AsuC2I	CCSGG	4 cut(s)	26, 450, 451, 1259
AvaI	CYCGRG	2 cut(s)	47, 449
AvaII	GGWCC	5 cut(s)	83, 356, 446, 744, 2276
BaeGI	GKGCMC	1 cut(s)	329
BanI	GGYRCC	1 cut(s)	158
BanII	GRGCYC	4 cut(s)	550, 975, 2356, 2580
BauI	CACGAG	1 cut(s)	2607
BbsI	GAAGAC	1 cut(s)	1409
Bbv12I	GWGCWC	5 cut(s)	640, 832, 975, 2356, 2580
BbvCI	CCTCAGC	1 cut(s)	1281
BceAI	ACGGC	4 cut(s)	95, 148, 478, 2454
BciT130I	CCWGG	9 cut(s)	81, 198, 253, 756, 1067, 1567, 1661, 2280, 2393
BciVI	GTATCC	4 cut(s)	547, 772, 1150, 1941
BclI	TGATCA	1 cut(s)	2403
BcnI	CCSGG	4 cut(s)	26, 450, 451, 1259
BcoDI	GTCTC	9 cut(s)	44, 146, 1038, 1471, 1581, 1824, 1889, 2254, 2528
BfaI	CTAG	5 cut(s)	264, 582, 615, 695, 1055
BfmI	CTRYAG	3 cut(s)	276, 1452, 2148
BfuAI	ACCTGC	1 cut(s)	269
BfuI	GTATCC	4 cut(s)	547, 772, 1150, 1941
BglI	GCCNNNNNGGC	1 cut(s)	1448
BglII	AGATCT	1 cut(s)	2135
BlpI	GCTNAGC	1 cut(s)	1698
BmcAI	AGTACT	1 cut(s)	1174
Bme18I	GGWCC	5 cut(s)	83, 356, 446, 744, 2276
BmeT110I	CYCGRG	2 cut(s)	47, 449
BmgT120I	GGNCC	9 cut(s)	83, 134, 182, 356, 446, 452, 744, 2276, 2297
BmiI	GGNNCC	7 cut(s)	30, 136, 160, 184, 448, 746, 2490
BmsI	GCATC	6 cut(s)	210, 529, 1010, 1328, 2179, 2559
BoxI	GACNNNNGTC	1 cut(s)	1483
BpiI	GAAGAC	1 cut(s)	1409
BplI	GAGNNNNNCTC	4 cut(s)	289, 321, 1196, 1228
BpmI	CTGGAG	1 cut(s)	912
Bpu10I	CCTNAGC	1 cut(s)	1281
Bpu1102I	GCTNAGC	1 cut(s)	1698
BpuEI	CTTGAG	2 cut(s)	1246, 2278
BpuMI	CCSGG	4 cut(s)	26, 450, 451, 1259
BsaAI	YACGTR	2 cut(s)	388, 1955
BsaBI	GATNNNNATC	2 cut(s)	1816, 2631
BsaHI	GRCGYC	1 cut(s)	75
BsaI	GGTCTC	3 cut(s)	44, 1889, 2254
BsaJI	CCNNGG	9 cut(s)	14, 79, 130, 196, 296, 449, 1401, 2391, 2392
BsaWI	WCCGGW	1 cut(s)	2530
Bsc4I	CCNNNNNNNGG	4 cut(s)	258, 461, 472, 2609
Bse1I	ACTGG	6 cut(s)	633, 1143, 1225, 1290, 1634, 2466
Bse8I	GATNNNNATC	2 cut(s)	1816, 2631
BseBI	CCWGG	9 cut(s)	81, 198, 253, 756, 1067, 1567, 1661, 2280, 2393
BseDI	CCNNGG	9 cut(s)	14, 79, 130, 196, 296, 449, 1401, 2391, 2392
BseGI	GGATG	8 cut(s)	144, 1047, 1476, 1546, 2194, 2377, 2503, 2574
BseJI	GATNNNNATC	2 cut(s)	1816, 2631
BseLI	CCNNNNNNNGG	4 cut(s)	258, 461, 472, 2609
BseMII	CTCAG	5 cut(s)	657, 1113, 1194, 1272, 1689
BseNI	ACTGG	6 cut(s)	633, 1143, 1225, 1290, 1634, 2466
BseRI	GAGGAG	2 cut(s)	178, 2508
BseSI	GKGCMC	1 cut(s)	329
BseYI	CCCAGC	2 cut(s)	212, 362
Bsh1236I	CGCG	1 cut(s)	311
Bsh1285I	CGRYCG	2 cut(s)	1876, 2618
BshFI	GGCC	7 cut(s)	135, 183, 274, 454, 465, 1621, 2299
BshNI	GGYRCC	1 cut(s)	158
BsiEI	CGRYCG	2 cut(s)	1876, 2618
BsiHKAI	GWGCWC	5 cut(s)	640, 832, 975, 2356, 2580
BsiHKCI	CYCGRG	2 cut(s)	47, 449
BsiSI	CCGG	4 cut(s)	26, 450, 1258, 2531
BslFI	GGGAC	4 cut(s)	957, 1131, 2409, 2449
BslI	CCNNNNNNNGG	4 cut(s)	258, 461, 472, 2609
BsmAI	GTCTC	9 cut(s)	44, 146, 1038, 1471, 1581, 1824, 1889, 2254, 2528
BsmBI	CGTCTC	1 cut(s)	146
BsmFI	GGGAC	4 cut(s)	957, 1131, 2409, 2449
BsmI	GAATGC	3 cut(s)	232, 367, 2012
BsnI	GGCC	7 cut(s)	135, 183, 274, 454, 465, 1621, 2299
Bso31I	GGTCTC	3 cut(s)	44, 1889, 2254
BsoBI	CYCGRG	2 cut(s)	47, 449
Bsp1286I	GDGCHC	7 cut(s)	329, 550, 640, 832, 975, 2356, 2580
Bsp1407I	TGTACA	1 cut(s)	433
Bsp1720I	GCTNAGC	1 cut(s)	1698
Bsp19I	CCATGG	1 cut(s)	14
BspANI	GGCC	7 cut(s)	135, 183, 274, 454, 465, 1621, 2299
BspCNI	CTCAG	5 cut(s)	656, 1114, 1195, 1273, 1690
BspFNI	CGCG	1 cut(s)	311
BspHI	TCATGA	1 cut(s)	2356
BspLI	GGNNCC	7 cut(s)	30, 136, 160, 184, 448, 746, 2490
BspMAI	CTGCAG	1 cut(s)	280
BspMI	ACCTGC	1 cut(s)	269
BspPI	GGATC	5 cut(s)	953, 1122, 1500, 2220, 2222
BspT107I	GGYRCC	1 cut(s)	158
BspTNI	GGTCTC	3 cut(s)	44, 1889, 2254
BsrGI	TGTACA	1 cut(s)	433
BsrI	ACTGG	6 cut(s)	633, 1143, 1225, 1290, 1634, 2466
BssECI	CCNNGG	9 cut(s)	14, 79, 130, 196, 296, 449, 1401, 2391, 2392
BssNI	GRCGYC	1 cut(s)	75
BssSI	CACGAG	1 cut(s)	2607
BssT1I	CCWWGG	1 cut(s)	14
Bst2BI	CACGAG	1 cut(s)	2607
Bst2UI	CCWGG	9 cut(s)	81, 198, 253, 756, 1067, 1567, 1661, 2280, 2393
Bst4CI	ACNGT	7 cut(s)	505, 1138, 1482, 1726, 1776, 2246, 2400
Bst6I	CTCTTC	2 cut(s)	667, 2526
BstACI	GRCGYC	1 cut(s)	75
BstAPI	GCANNNNNTGC	3 cut(s)	869, 1292, 2447
BstAUI	TGTACA	1 cut(s)	433
BstBAI	YACGTR	2 cut(s)	388, 1955
BstC8I	GCNNGC	4 cut(s)	276, 640, 1305, 2194
BstDEI	CTNAG	8 cut(s)	478, 643, 833, 1037, 1122, 1203, 1281, 1698
BstDSI	CCRYGG	2 cut(s)	14, 130
BstEII	GGTNACC	1 cut(s)	217
BstF5I	GGATG	8 cut(s)	144, 1047, 1476, 1546, 2194, 2377, 2503, 2574
BstFNI	CGCG	1 cut(s)	311
BstHHI	GCGC	4 cut(s)	95, 806, 1312, 1794
BstMAI	GTCTC	9 cut(s)	44, 146, 1038, 1471, 1581, 1824, 1889, 2254, 2528
BstMCI	CGRYCG	2 cut(s)	1876, 2618
BstNI	CCWGG	9 cut(s)	81, 198, 253, 756, 1067, 1567, 1661, 2280, 2393
BstNSI	RCATGY	2 cut(s)	788, 818
BstPAI	GACNNNNGTC	1 cut(s)	1483
BstPI	GGTNACC	1 cut(s)	217
BstSFI	CTRYAG	3 cut(s)	276, 1452, 2148
BstSLI	GKGCMC	1 cut(s)	329
BstUI	CGCG	1 cut(s)	311
BstV2I	GAAGAC	1 cut(s)	1409
BstX2I	RGATCY	3 cut(s)	1127, 2135, 2212
BstXI	CCANNNNNNTGG	1 cut(s)	2185
BstYI	RGATCY	3 cut(s)	1127, 2135, 2212
BsuI	GTATCC	4 cut(s)	547, 772, 1150, 1941
BsuRI	GGCC	7 cut(s)	135, 183, 274, 454, 465, 1621, 2299
BtgI	CCRYGG	2 cut(s)	14, 130
BtsCI	GGATG	8 cut(s)	144, 1047, 1476, 1546, 2194, 2377, 2503, 2574
BtsI	GCAGTG	1 cut(s)	882
BtsIMutI	CAGTG	6 cut(s)	640, 740, 882, 1297, 1916, 2251
BveI	ACCTGC	1 cut(s)	269
Cac8I	GCNNGC	4 cut(s)	276, 640, 1305, 2194
CaiI	CAGNNNCTG	1 cut(s)	1322
CciI	TCATGA	1 cut(s)	2356
CfoI	GCGC	4 cut(s)	95, 806, 1312, 1794
Cfr13I	GGNCC	9 cut(s)	83, 134, 182, 356, 446, 452, 744, 2276, 2297
Cfr9I	CCCGGG	1 cut(s)	449
CseI	GACGC	4 cut(s)	83, 710, 968, 2570
CsiI	ACCWGGT	2 cut(s)	754, 2278
Csp6I	GTAC	5 cut(s)	42, 434, 1173, 1209, 1561
CspCI	CAANNNNNGTGG	4 cut(s)	151, 186, 1447, 1482
CviQI	GTAC	5 cut(s)	42, 434, 1173, 1209, 1561
DdeI	CTNAG	8 cut(s)	478, 643, 833, 1037, 1122, 1203, 1281, 1698
DraI	TTTAAA	1 cut(s)	415
DraIII	CACNNNGTG	2 cut(s)	427, 758
EaeI	YGGCCR	1 cut(s)	463
Eam1104I	CTCTTC	2 cut(s)	667, 2526
EarI	CTCTTC	2 cut(s)	667, 2526
EciI	GGCGGA	1 cut(s)	181
Ecl136II	GAGCTC	3 cut(s)	973, 2354, 2578
Eco130I	CCWWGG	1 cut(s)	14
Eco147I	AGGCCT	2 cut(s)	274, 1621
Eco24I	GRGCYC	4 cut(s)	550, 975, 2356, 2580
Eco31I	GGTCTC	3 cut(s)	44, 1889, 2254
Eco32I	GATATC	2 cut(s)	494, 1978
Eco47I	GGWCC	5 cut(s)	83, 356, 446, 744, 2276
Eco53kI	GAGCTC	3 cut(s)	973, 2354, 2578
Eco57I	CTGAAG	5 cut(s)	1116, 1356, 1715, 1940, 2507
Eco88I	CYCGRG	2 cut(s)	47, 449
Eco91I	GGTNACC	1 cut(s)	217
EcoICRI	GAGCTC	3 cut(s)	973, 2354, 2578
EcoO109I	RGGNCCY	3 cut(s)	356, 446, 2276
EcoO65I	GGTNACC	1 cut(s)	217
EcoRII	CCWGG	9 cut(s)	79, 196, 251, 754, 1065, 1565, 1659, 2278, 2391
EcoRV	GATATC	2 cut(s)	494, 1978
EcoT14I	CCWWGG	1 cut(s)	14
EcoT22I	ATGCAT	3 cut(s)	577, 723, 2121
EcoT38I	GRGCYC	4 cut(s)	550, 975, 2356, 2580
ErhI	CCWWGG	1 cut(s)	14
Esp3I	CGTCTC	1 cut(s)	146
FaqI	GGGAC	4 cut(s)	957, 1131, 2409, 2449
FauI	CCCGC	2 cut(s)	1819, 2426
FbaI	TGATCA	1 cut(s)	2403
FblI	GTMKAC	1 cut(s)	2614
FokI	GGATG	8 cut(s)	131, 1034, 1483, 1533, 2201, 2384, 2490, 2581
FriOI	GRGCYC	4 cut(s)	550, 975, 2356, 2580
FspBI	CTAG	5 cut(s)	264, 582, 615, 695, 1055
FspI	TGCGCA	1 cut(s)	1311
GlaI	GCGC	4 cut(s)	94, 805, 1311, 1793
GsaI	CCCAGC	2 cut(s)	216, 366
GsuI	CTGGAG	1 cut(s)	912
HaeIII	GGCC	7 cut(s)	135, 183, 274, 454, 465, 1621, 2299
HapII	CCGG	4 cut(s)	26, 450, 1258, 2531
HgaI	GACGC	4 cut(s)	83, 710, 968, 2570
HhaI	GCGC	4 cut(s)	95, 806, 1312, 1794
Hin1I	GRCGYC	1 cut(s)	75
Hin6I	GCGC	4 cut(s)	93, 804, 1310, 1792
HinP1I	GCGC	4 cut(s)	93, 804, 1310, 1792
HincII	GTYRAC	1 cut(s)	2615
HindII	GTYRAC	1 cut(s)	2615
HindIII	AAGCTT	1 cut(s)	2165
HinfI	GANTC	4 cut(s)	1522, 1664, 2037, 2050
HpaII	CCGG	4 cut(s)	26, 450, 1258, 2531
Hpy166II	GTNNAC	6 cut(s)	86, 744, 1199, 1563, 1945, 2615
Hpy188III	TCNNGA	8 cut(s)	47, 49, 551, 684, 1934, 2357, 2543, 2630
Hpy8I	GTNNAC	6 cut(s)	86, 744, 1199, 1563, 1945, 2615
Hpy99I	CGWCG	6 cut(s)	71, 74, 77, 116, 984, 2564
HpyAV	CCTTC	9 cut(s)	167, 300, 590, 777, 848, 972, 1091, 1341, 1591
HpyCH4III	ACNGT	7 cut(s)	505, 1138, 1482, 1726, 1776, 2246, 2400
HpyCH4IV	ACGT	3 cut(s)	387, 1838, 1954
HpyF3I	CTNAG	8 cut(s)	478, 643, 833, 1037, 1122, 1203, 1281, 1698
HpySE526I	ACGT	3 cut(s)	387, 1838, 1954
Hsp92I	GRCGYC	1 cut(s)	75
HspAI	GCGC	4 cut(s)	93, 804, 1310, 1792
Ksp22I	TGATCA	1 cut(s)	2403
LmnI	GCTCC	5 cut(s)	34, 970, 1717, 1914, 2575
LweI	GCATC	6 cut(s)	210, 529, 1010, 1328, 2179, 2559
MabI	ACCWGGT	2 cut(s)	754, 2278
MaeI	CTAG	5 cut(s)	264, 582, 615, 695, 1055
MaeII	ACGT	3 cut(s)	387, 1838, 1954
MaeIII	GTNAC	8 cut(s)	217, 499, 817, 919, 1394, 2287, 2308, 2526
MboII	GAAGA	8 cut(s)	684, 1179, 1349, 1409, 1721, 1757, 1933, 2513
MflI	RGATCY	3 cut(s)	1127, 2135, 2212
MhlI	GDGCHC	7 cut(s)	329, 550, 640, 832, 975, 2356, 2580
MlyI	GAGTC	1 cut(s)	2044
MmeI	TCCRAC	1 cut(s)	56
Mph1103I	ATGCAT	3 cut(s)	577, 723, 2121
MroXI	GAANNNNTTC	2 cut(s)	1186, 1655
MseI	TTAA	8 cut(s)	398, 414, 1115, 1749, 1787, 1949, 2207, 2498
MslI	CAYNNNNRTG	4 cut(s)	237, 726, 2183, 2361
MspA1I	CMGCKG	4 cut(s)	317, 510, 998, 1735
MspI	CCGG	4 cut(s)	26, 450, 1258, 2531
Mva1269I	GAATGC	3 cut(s)	232, 367, 2012
MvaI	CCWGG	9 cut(s)	81, 198, 253, 756, 1067, 1567, 1661, 2280, 2393
MvnI	CGCG	1 cut(s)	311
NciI	CCSGG	4 cut(s)	26, 450, 451, 1259
NcoI	CCATGG	1 cut(s)	14
NlaIV	GGNNCC	7 cut(s)	30, 136, 160, 184, 448, 746, 2490
NmuCI	GTSAC	6 cut(s)	217, 919, 1394, 2287, 2308, 2526
NsbI	TGCGCA	1 cut(s)	1311
NsiI	ATGCAT	3 cut(s)	577, 723, 2121
NspI	RCATGY	2 cut(s)	788, 818
PaeR7I	CTCGAG	1 cut(s)	47
PagI	TCATGA	1 cut(s)	2356
PaqCI	CACCTGC	1 cut(s)	269
PasI	CCCWGGG	1 cut(s)	2392
PceI	AGGCCT	2 cut(s)	274, 1621
PciI	ACATGT	1 cut(s)	814
PctI	GAATGC	3 cut(s)	232, 367, 2012
PdmI	GAANNNNTTC	2 cut(s)	1186, 1655
PfeI	GAWTC	3 cut(s)	1522, 1664, 2037
PflFI	GACNNNGTC	1 cut(s)	2280
PflMI	CCANNNNNTGG	2 cut(s)	258, 472
PfoI	TCCNGGA	2 cut(s)	1257, 1659
Ple19I	CGATCG	1 cut(s)	1876
PleI	GAGTC	1 cut(s)	2044
PpsI	GAGTC	1 cut(s)	2044
Ppu21I	YACGTR	2 cut(s)	388, 1955
PpuMI	RGGWCCY	3 cut(s)	356, 446, 2276
PscI	ACATGT	1 cut(s)	814
PshAI	GACNNNNGTC	1 cut(s)	1483
PshBI	ATTAAT	1 cut(s)	2498
Psp124BI	GAGCTC	3 cut(s)	975, 2356, 2580
Psp5II	RGGWCCY	3 cut(s)	356, 446, 2276
Psp6I	CCWGG	9 cut(s)	79, 196, 251, 754, 1065, 1565, 1659, 2278, 2391
PspEI	GGTNACC	1 cut(s)	217
PspFI	CCCAGC	2 cut(s)	212, 362
PspGI	CCWGG	9 cut(s)	79, 196, 251, 754, 1065, 1565, 1659, 2278, 2391
PspN4I	GGNNCC	7 cut(s)	30, 136, 160, 184, 448, 746, 2490
PspPI	GGNCC	9 cut(s)	83, 134, 182, 356, 446, 452, 744, 2276, 2297
PspPPI	RGGWCCY	3 cut(s)	356, 446, 2276
PstI	CTGCAG	1 cut(s)	280
PstNI	CAGNNNCTG	1 cut(s)	1322
PsuI	RGATCY	3 cut(s)	1127, 2135, 2212
PsyI	GACNNNGTC	1 cut(s)	2280
PvuI	CGATCG	1 cut(s)	1876
PvuII	CAGCTG	3 cut(s)	317, 998, 1735
RsaI	GTAC	5 cut(s)	43, 435, 1174, 1210, 1562
RsaNI	GTAC	5 cut(s)	42, 434, 1173, 1209, 1561
RseI	CAYNNNNRTG	4 cut(s)	237, 726, 2183, 2361
SacI	GAGCTC	3 cut(s)	975, 2356, 2580
SalI	GTCGAC	1 cut(s)	2613
SaqAI	TTAA	8 cut(s)	398, 414, 1115, 1749, 1787, 1949, 2207, 2498
Sau96I	GGNCC	9 cut(s)	83, 134, 182, 356, 446, 452, 744, 2276, 2297
SbfI	CCTGCAGG	1 cut(s)	280
ScaI	AGTACT	1 cut(s)	1174
SchI	GAGTC	1 cut(s)	2044
SdaI	CCTGCAGG	1 cut(s)	280
SduI	GDGCHC	7 cut(s)	329, 550, 640, 832, 975, 2356, 2580
SexAI	ACCWGGT	2 cut(s)	754, 2278
SfaNI	GCATC	6 cut(s)	210, 529, 1010, 1328, 2179, 2559
SfcI	CTRYAG	3 cut(s)	276, 1452, 2148
Sfr274I	CTCGAG	1 cut(s)	47
SinI	GGWCC	5 cut(s)	83, 356, 446, 744, 2276
SlaI	CTCGAG	1 cut(s)	47
SmaI	CCCGGG	1 cut(s)	451
SmiI	ATTTAAAT	1 cut(s)	415
SmiMI	CAYNNNNRTG	4 cut(s)	237, 726, 2183, 2361
SmlI	CTYRAG	3 cut(s)	47, 1225, 2257
SmoI	CTYRAG	3 cut(s)	47, 1225, 2257
Sse8387I	CCTGCAGG	1 cut(s)	280
SseBI	AGGCCT	2 cut(s)	274, 1621
SspMI	CTAG	5 cut(s)	264, 582, 615, 695, 1055
SstI	GAGCTC	3 cut(s)	975, 2356, 2580
StuI	AGGCCT	2 cut(s)	274, 1621
StyI	CCWWGG	1 cut(s)	14
SwaI	ATTTAAAT	1 cut(s)	415
TaaI	ACNGT	7 cut(s)	505, 1138, 1482, 1726, 1776, 2246, 2400
TaiI	ACGT	3 cut(s)	390, 1841, 1957
TatI	WGTACW	3 cut(s)	433, 1172, 1560
TauI	GCSGC	4 cut(s)	119, 314, 351, 791
TfiI	GAWTC	3 cut(s)	1522, 1664, 2037
Tru1I	TTAA	8 cut(s)	398, 414, 1115, 1749, 1787, 1949, 2207, 2498
Tru9I	TTAA	8 cut(s)	398, 414, 1115, 1749, 1787, 1949, 2207, 2498
TscAI	CASTG	6 cut(s)	640, 740, 889, 1297, 1923, 2251
TseFI	GTSAC	6 cut(s)	217, 919, 1394, 2287, 2308, 2526
Tsp45I	GTSAC	6 cut(s)	217, 919, 1394, 2287, 2308, 2526
TspMI	CCCGGG	1 cut(s)	449
TspRI	CASTG	6 cut(s)	640, 740, 889, 1297, 1923, 2251
Tth111I	GACNNNGTC	1 cut(s)	2280
Van91I	CCANNNNNTGG	2 cut(s)	258, 472
VpaK11BI	GGWCC	5 cut(s)	83, 356, 446, 744, 2276
VspI	ATTAAT	1 cut(s)	2498
XapI	RAATTY	4 cut(s)	410, 1078, 1240, 2315
XceI	RCATGY	2 cut(s)	788, 818
XhoI	CTCGAG	1 cut(s)	47
XmaI	CCCGGG	1 cut(s)	449
XmiI	GTMKAC	1 cut(s)	2614
XmnI	GAANNNNTTC	2 cut(s)	1186, 1655
XspI	CTAG	5 cut(s)	264, 582, 615, 695, 1055
ZrmI	AGTACT	1 cut(s)	1174
Zsp2I	ATGCAT	3 cut(s)	577, 723, 2121

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.