Rroxscaffold_7G00175230

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0006473
Physical Location & Seq
Reverse (-)
14826381 .. 14833399
7019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00175230.1

Sequence Viewer

Length: 2655 bp
ATGAGTTCGAAACCATGGCTGTACCCGGCTCCTACCTACCGTACTCTCGAGACCTATTGGGATACCGACGACGACGCGCCCGGCCCGCGATGCGGTCACACGCTCACGGCCGTCGCCGCCACCAAGACCCACGGCCCCCGCCTTATTCTCTTCGGTGGTGCCACCGCCATTGAAGGCGGCGCCGCCTCCTCCGCCCCCGGCATCAGGCTAGCGGGTGTGACCAATTCTGTTCACTCCTACGATGTTCTCACCAGAAAGTGGACTAGAATCAGGCCGGCTGGTGAGCCACCGTCTCCGAGGGCGGCGCACGCTGCTGCTGCAGTGGGGACGATGGTGGTCTTTCAGGGTGGCATTGGTCCTGCTGGGCACTCCACGGATGATCTCTTTGTGCTTGACATGGATAAGTTCAAGTGGCACAGAGTGGTTGTGCAAGGACAAGGACCTGGGCCTCGCTATGGCCATGTTATGGACTTAGTTGCTCAAAGATATCTTGTTACTGTCAGCGGCAATGATGGAAAAAGAGTTCTTTCTGATTCCTGGGCTCTGGATACTGCACAGAAACCTTACGTATGGGCTAAGCTGAGTCCAGAAGGGGATAGGCCTTCTGCTAGAATGTATGCAACAGCCAGTGCCCGCTCAGATGGCATGTTTTTGCTTTGTGGCGGAAGAGACGCTTCCGGGGCGCCTCTAGCTGATGCTTATGGACTGCTCATGCATAGAAATGGTCAGTGGGAGTGGACTCTCGCACCGGGAGTGTCTCCTTCACCAAGATATCAACATGCTGCGGTTTTTGTTGGTGCAAGATTACATGTAACGGGAGGTGTTCTCAGGGGAGGACGTGCTGTAGAAGGGGAAGGAGCTATTGCAGTATTGGACACTGCCGCTGGAGTTTGGTTAGATAGAAGCGGGCTTGTGACCTCCTCACGGACAGGCAAGGGACAAAATGACTATGATCCCCCTTTGGAGCTTATGCGTCGTTGTCGCCATGCAGCCGCATCTCTTGGTGTTCGTATTTACATTTATGGCGGTCTCAAGGACATCCTGTTAGATGATTTCCTGGTTGCGGAAAATTCATCATTTCAATCTGAAACTAATTCTCCTGTATCAACTTCTGAGAAATCCCCATCTTTAACAAGCCCGAGAGCCTTTCATGCCAACACAAGTTCTTTTGGTGTATCACCATCTTCGGATAGTGAATCTGAGATACCCTCATCTAGTGGCTTGAGCATGGATAAAAATTCCATGGAGAAACTACGGCAGGCTTCTGCTGCCGAAGCTGAGGCAGCTAGTGCTGTTTGGCAAGCTGTGCAGTCCCAATCTTCCCCTCCTCCTGAAGAAACTTCTGTTTCAGATGACAACTCACAAATTGCAGAAACACTTTCGGATGGTAGTGACACGGAGGCAGATGTTCGCCTTCATCCCAGAGCTGTTGTTGTTGCCAAAGAGGCTGTAGGCAATTTGGGTGGCATGGTAAGACAGTTGTCTTTGGATCAATTCGAAAATGAGAGCAGACGAATGATTCCAATGAATAACGACTTATCATATCCTAACAAAAAGTTTACCAGGCAGAAGTCCCCACAGGGCTTACATAAGAAGATAATATCTACATTGCTGAGGCCAAGGAACTGGAAACCTCCGGTTAATAGGAAGTTTTTCCTCGATTCTTACGAAGTGGGAGAACTTTGTTATGCTGCTGAACAGATCTTTATGCAGGAGCAAACAGTTCTCCAGCTGAAAGCTCCTGTTAAAGTATTTGGCGATCTTCATGGACAGTTTGGTGATTTAATGCGCTTGTTTGATGAATATGGATTTCCATCTGCTGCAGGAGACATAACGTACATTGACTACTTGTTTTTGGGGGATTATGTTGATCGAGGACAGCACAGCTTGGAGACAATAACTCTACTCCTTGCACTCAAGATTGAGTATCCTGATAATGTTCACTTAATACGTGGAAACCATGAGGCTGCTGATATCAACGCACTCTTTGGTTTTCGTATTGAATGCATTGAGAGAATGGGAGAGAGTGATGGAATATGGGCATGGACAAGGTTCAATCAACTTTTCAACTACCTCCCACTGGCTGCACTAATTGAAAAGAAAATCATCTGTATGCATGGTGGCATTGGAAGATCTATAAATTCTGTAGAACAAATAGAGAAGCTTGAAAGACCCATTACAATGGATGCTGGGTCTATAATTTTAATGGATCTTCTATGGTCTGATCCTACAGAAAACGATGGTGTAGAGGGTCTCAGACCAAATGCAAGAGGGCCTGGTCTTGTCACTTTTGGGCCTGATCGTGTCACGGACTTCTGTAAGAGGAACAAATTACAGCTCATTATAAGAGCTCATGAATGTGTTATGGATGGTTTTGAACGGTTTGCCCAGGGACAATTGATCACTCTTTTTTCTGCCACCAACTATTGTGGAACGGCAAACAATGCTGGTGCAATACTGGTGGTTGGCAGGGGGCTGGTTGTAGTTCCAAAACTAATTCATCCGCTGCCACCTCCTCTTCAGTCGCCGGAGACATCTCCTGAACGCGTTATAGATGACACATGGATGCAGGAGCTAAACATTCAGAGGCCGCCAACTCCTACTCGTGGTCGACCACAGCCTGATCTTGATCGGAACTCACTTGCATATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4 Metallophos STPPase_N

Protein Analysis

884

Amino Acids

96.54

Weight (kDa)

6.0

Isoelectric Point (pI)

41.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 21 - 221 2.1e-33 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 22 - 186 1.6e-25 FBX42, beta-propeller domain
Kelch_HCF PF13854 72 - 343 4e-15 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 174 - 314 2e-10 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 190 - 385 2.7e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_TYW4 PF13418 201 - 276 9.2e-07 tRNA wybutosine-synthesizing protein 4, beta-propeller
Metallophos PF00149 581 - 788 2.6e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2345
AccB1I GGYRCC 3 cut(s) 158, 179, 682
AccB7I CCANNNNNTGG 2 cut(s) 258, 466
AccBSI CCGCTC 1 cut(s) 636
AccI GTMKAC 1 cut(s) 2611
AccII CGCG 3 cut(s) 77, 88, 2547
AclWI GGATC 4 cut(s) 947, 1497, 2217, 2219
AcoI YGGCCR 2 cut(s) 108, 457
AcsI RAATTY 3 cut(s) 1069, 1237, 2140
AcuI CTGAAG 2 cut(s) 1353, 2504
AcyI GRCGYC 2 cut(s) 180, 683
AdeI CACNNNGTG 1 cut(s) 421
AfaI GTAC 3 cut(s) 23, 43, 1838
AfiI CCNNNNNNNGG 9 cut(s) 92, 204, 258, 455, 466, 924, 1063, 2080, 2606
AflIII ACRYGT 2 cut(s) 808, 2545
AgsI TTSAA 9 cut(s) 173, 409, 1082, 2003, 2056, 2068, 2096, 2168, 2378
AjiI CACGTC 1 cut(s) 839
AjnI CCWGG 6 cut(s) 442, 536, 1056, 1562, 2275, 2388
AloI GAACNNNNNNTCC 2 cut(s) 507, 539
Alw21I GWGCWC 1 cut(s) 2353
Alw26I GTCTC 9 cut(s) 44, 297, 663, 762, 1034, 1821, 1886, 2258, 2525
AlwI GGATC 4 cut(s) 947, 1497, 2217, 2219
Ama87I CYCGRG 2 cut(s) 47, 1138
ApoI RAATTY 3 cut(s) 1069, 1237, 2140
Asp700I GAANNNNTTC 1 cut(s) 1652
AspLEI GCGC 5 cut(s) 79, 182, 307, 685, 1791
AspS9I GGNCC 7 cut(s) 83, 134, 356, 440, 446, 2273, 2294
AsuC2I CCSGG 5 cut(s) 26, 81, 198, 679, 750
AsuHPI GGTGA 5 cut(s) 241, 293, 756, 1170, 1790
AsuII TTCGAA 2 cut(s) 8, 1497
AsuNHI GCTAGC 1 cut(s) 208
AvaI CYCGRG 2 cut(s) 47, 1138
AvaII GGWCC 2 cut(s) 356, 440
BaeGI GKGCMC 2 cut(s) 369, 634
BalI TGGCCA 1 cut(s) 459
BanI GGYRCC 3 cut(s) 158, 179, 682
BanII GRGCYC 2 cut(s) 544, 2353
BauI CACGAG 1 cut(s) 2604
Bbv12I GWGCWC 1 cut(s) 2353
BbvCI CCTCAGC 2 cut(s) 1278, 1613
BceAI ACGGC 5 cut(s) 95, 123, 148, 1271, 2451
BciT130I CCWGG 6 cut(s) 444, 538, 1058, 1564, 2277, 2390
BciVI GTATCC 3 cut(s) 55, 541, 1938
BclI TGATCA 1 cut(s) 2400
BcnI CCSGG 5 cut(s) 26, 81, 198, 679, 750
BcoDI GTCTC 9 cut(s) 44, 297, 663, 762, 1034, 1821, 1886, 2258, 2525
BfaI CTAG 6 cut(s) 209, 264, 609, 689, 1215, 1287
BfmI CTRYAG 6 cut(s) 318, 843, 1449, 1821, 2145, 2229
BfoI RGCGCY 2 cut(s) 183, 686
BfuI GTATCC 3 cut(s) 55, 541, 1938
BglI GCCNNNNNGGC 1 cut(s) 1445
BglII AGATCT 2 cut(s) 1701, 2132
BlpI GCTNAGC 1 cut(s) 576
Bme18I GGWCC 2 cut(s) 356, 440
BmeT110I CYCGRG 2 cut(s) 47, 1138
BmgBI CACGTC 1 cut(s) 839
BmgT120I GGNCC 7 cut(s) 83, 134, 356, 440, 446, 2273, 2294
BmiI GGNNCC 5 cut(s) 30, 136, 160, 181, 684
BmsI GCATC 6 cut(s) 80, 210, 685, 1004, 2176, 2556
BmtI GCTAGC 1 cut(s) 212
BoxI GACNNNNGTC 1 cut(s) 1480
BplI GAGNNNNNCTC 4 cut(s) 810, 842, 1193, 1225
BpmI CTGGAG 2 cut(s) 906, 1712
Bpu10I CCTNAGC 2 cut(s) 1278, 1613
Bpu1102I GCTNAGC 1 cut(s) 576
Bpu14I TTCGAA 2 cut(s) 8, 1497
BpuEI CTTGAG 3 cut(s) 1016, 1243, 1901
BpuMI CCSGG 5 cut(s) 26, 81, 198, 679, 750
BsaAI YACGTR 2 cut(s) 568, 1952
BsaBI GATNNNNATC 2 cut(s) 1813, 2628
BsaHI GRCGYC 2 cut(s) 180, 683
BsaI GGTCTC 3 cut(s) 44, 1034, 2258
BsaWI WCCGGW 1 cut(s) 1636
BsaXI ACNNNNNCTCC 2 cut(s) 1081, 1111
Bsc4I CCNNNNNNNGG 9 cut(s) 92, 204, 258, 455, 466, 924, 1063, 2080, 2606
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 4 cut(s) 627, 1631, 2085, 2463
Bse3DI GCAATG 2 cut(s) 514, 1607
Bse8I GATNNNNATC 2 cut(s) 1813, 2628
BseBI CCWGG 6 cut(s) 444, 538, 1058, 1564, 2277, 2390
BseGI GGATG 8 cut(s) 382, 1038, 1390, 1417, 2191, 2374, 2500, 2571
BseJI GATNNNNATC 2 cut(s) 1813, 2628
BseLI CCNNNNNNNGG 9 cut(s) 92, 204, 258, 455, 466, 924, 1063, 2080, 2606
BseMI GCAATG 2 cut(s) 514, 1607
BseMII CTCAG 8 cut(s) 572, 651, 841, 1104, 1191, 1269, 1604, 2269
BseNI ACTGG 4 cut(s) 627, 1631, 2085, 2463
BseRI GAGGAG 4 cut(s) 178, 910, 1317, 2505
BseSI GKGCMC 2 cut(s) 369, 634
BseX3I CGGCCG 1 cut(s) 108
BseYI CCCAGC 2 cut(s) 362, 2189
BsgI GTGCAG 3 cut(s) 537, 1328, 2070
Bsh1236I CGCG 3 cut(s) 77, 88, 2547
Bsh1285I CGRYCG 1 cut(s) 111
BshNI GGYRCC 3 cut(s) 158, 179, 682
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 1 cut(s) 2353
BsiHKCI CYCGRG 2 cut(s) 47, 1138
BsiSI CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1637, 2528
BslFI GGGAC 5 cut(s) 340, 951, 1297, 1558, 2406
BslI CCNNNNNNNGG 9 cut(s) 92, 204, 258, 455, 466, 924, 1063, 2080, 2606
BsmAI GTCTC 9 cut(s) 44, 297, 663, 762, 1034, 1821, 1886, 2258, 2525
BsmBI CGTCTC 2 cut(s) 297, 663
BsmFI GGGAC 5 cut(s) 340, 951, 1297, 1558, 2406
BsmI GAATGC 1 cut(s) 2009
Bso31I GGTCTC 3 cut(s) 44, 1034, 2258
BsoBI CYCGRG 2 cut(s) 47, 1138
Bsp119I TTCGAA 2 cut(s) 8, 1497
Bsp1286I GDGCHC 4 cut(s) 369, 544, 634, 2353
Bsp1720I GCTNAGC 1 cut(s) 576
Bsp19I CCATGG 2 cut(s) 14, 1242
BspCNI CTCAG 8 cut(s) 573, 650, 840, 1105, 1192, 1270, 1605, 2268
BspFNI CGCG 3 cut(s) 77, 88, 2547
BspHI TCATGA 1 cut(s) 2353
BspLI GGNNCC 5 cut(s) 30, 136, 160, 181, 684
BspMAI CTGCAG 2 cut(s) 322, 1825
BspOI GCTAGC 1 cut(s) 212
BspPI GGATC 4 cut(s) 947, 1497, 2217, 2219
BspT104I TTCGAA 2 cut(s) 8, 1497
BspT107I GGYRCC 3 cut(s) 158, 179, 682
BspTNI GGTCTC 3 cut(s) 44, 1034, 2258
BsrBI CCGCTC 1 cut(s) 636
BsrDI GCAATG 2 cut(s) 514, 1607
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 4 cut(s) 627, 1631, 2085, 2463
BssAI RCCGGY 1 cut(s) 274
BssNI GRCGYC 2 cut(s) 180, 683
BssSI CACGAG 1 cut(s) 2604
BssT1I CCWWGG 3 cut(s) 14, 1242, 1619
Bst2BI CACGAG 1 cut(s) 2604
Bst2UI CCWGG 6 cut(s) 444, 538, 1058, 1564, 2277, 2390
Bst4CI ACNGT 7 cut(s) 41, 291, 499, 1479, 1723, 1773, 2382
Bst6I CTCTTC 3 cut(s) 155, 661, 2523
BstACI GRCGYC 2 cut(s) 180, 683
BstAPI GCANNNNNTGC 3 cut(s) 1289, 1306, 2444
BstBAI YACGTR 2 cut(s) 568, 1952
BstBI TTCGAA 2 cut(s) 8, 1497
BstC8I GCNNGC 8 cut(s) 86, 210, 276, 309, 634, 908, 1260, 1302
BstDSI CCRYGG 4 cut(s) 14, 130, 372, 1242
BstF5I GGATG 8 cut(s) 382, 1038, 1390, 1417, 2191, 2374, 2500, 2571
BstFNI CGCG 3 cut(s) 77, 88, 2547
BstH2I RGCGCY 2 cut(s) 183, 686
BstHHI GCGC 5 cut(s) 79, 182, 307, 685, 1791
BstMAI GTCTC 9 cut(s) 44, 297, 663, 762, 1034, 1821, 1886, 2258, 2525
BstMCI CGRYCG 1 cut(s) 111
BstNI CCWGG 6 cut(s) 444, 538, 1058, 1564, 2277, 2390
BstNSI RCATGY 3 cut(s) 649, 782, 812
BstPAI GACNNNNGTC 1 cut(s) 1480
BstSFI CTRYAG 6 cut(s) 318, 843, 1449, 1821, 2145, 2229
BstSLI GKGCMC 2 cut(s) 369, 634
BstSNI TACGTA 1 cut(s) 568
BstUI CGCG 3 cut(s) 77, 88, 2547
BstX2I RGATCY 3 cut(s) 1701, 2132, 2209
BstXI CCANNNNNNTGG 2 cut(s) 1626, 2182
BstYI RGATCY 3 cut(s) 1701, 2132, 2209
BstZI CGGCCG 1 cut(s) 108
BsuI GTATCC 3 cut(s) 55, 541, 1938
BtgI CCRYGG 4 cut(s) 14, 130, 372, 1242
BtgZI GCGATG 1 cut(s) 103
BtrI CACGTC 1 cut(s) 839
BtsCI GGATG 8 cut(s) 382, 1038, 1390, 1417, 2191, 2374, 2500, 2571
BtsI GCAGTG 2 cut(s) 327, 876
BtsIMutI CAGTG 5 cut(s) 327, 634, 734, 876, 2078
Cac8I GCNNGC 8 cut(s) 86, 210, 276, 309, 634, 908, 1260, 1302
CciI TCATGA 1 cut(s) 2353
CfoI GCGC 5 cut(s) 79, 182, 307, 685, 1791
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 7 cut(s) 83, 134, 356, 440, 446, 2273, 2294
CseI GACGC 3 cut(s) 83, 680, 962
Csp6I GTAC 3 cut(s) 22, 42, 1837
CspCI CAANNNNNGTGG 8 cut(s) 151, 186, 1444, 1479, 2410, 2442, 2445, 2477
CviQI GTAC 3 cut(s) 22, 42, 1837
DinI GGCGCC 2 cut(s) 181, 684
DraIII CACNNNGTG 1 cut(s) 421
EaeI YGGCCR 2 cut(s) 108, 457
EagI CGGCCG 1 cut(s) 108
Eam1104I CTCTTC 3 cut(s) 155, 661, 2523
EarI CTCTTC 3 cut(s) 155, 661, 2523
EciI GGCGGA 2 cut(s) 181, 678
Ecl136II GAGCTC 1 cut(s) 2351
EclXI CGGCCG 1 cut(s) 108
Eco105I TACGTA 1 cut(s) 568
Eco130I CCWWGG 3 cut(s) 14, 1242, 1619
Eco147I AGGCCT 1 cut(s) 601
Eco24I GRGCYC 2 cut(s) 544, 2353
Eco31I GGTCTC 3 cut(s) 44, 1034, 2258
Eco32I GATATC 3 cut(s) 488, 773, 1975
Eco47I GGWCC 2 cut(s) 356, 440
Eco52I CGGCCG 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 2351
Eco57I CTGAAG 2 cut(s) 1353, 2504
Eco88I CYCGRG 2 cut(s) 47, 1138
EcoICRI GAGCTC 1 cut(s) 2351
EcoO109I RGGNCCY 2 cut(s) 440, 2273
EcoRII CCWGG 6 cut(s) 442, 536, 1056, 1562, 2275, 2388
EcoRV GATATC 3 cut(s) 488, 773, 1975
EcoT14I CCWWGG 3 cut(s) 14, 1242, 1619
EcoT22I ATGCAT 3 cut(s) 717, 2009, 2118
EcoT38I GRGCYC 2 cut(s) 544, 2353
EgeI GGCGCC 2 cut(s) 181, 684
EheI GGCGCC 2 cut(s) 181, 684
ErhI CCWWGG 3 cut(s) 14, 1242, 1619
Esp3I CGTCTC 2 cut(s) 297, 663
FalI AAGNNNNNCTT 2 cut(s) 658, 690
FaqI GGGAC 5 cut(s) 340, 951, 1297, 1558, 2406
FauI CCCGC 5 cut(s) 93, 146, 205, 641, 899
FbaI TGATCA 1 cut(s) 2400
FblI GTMKAC 1 cut(s) 2611
FokI GGATG 8 cut(s) 389, 1025, 1397, 1404, 2198, 2381, 2487, 2578
FriOI GRGCYC 2 cut(s) 544, 2353
FspBI CTAG 6 cut(s) 209, 264, 609, 689, 1215, 1287
GlaI GCGC 5 cut(s) 78, 181, 306, 684, 1790
GsaI CCCAGC 2 cut(s) 366, 2193
GsuI CTGGAG 2 cut(s) 906, 1712
HaeII RGCGCY 2 cut(s) 183, 686
HapII CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1637, 2528
HgaI GACGC 3 cut(s) 83, 680, 962
HhaI GCGC 5 cut(s) 79, 182, 307, 685, 1791
Hin1I GRCGYC 2 cut(s) 180, 683
Hin6I GCGC 5 cut(s) 77, 180, 305, 683, 1789
HinP1I GCGC 5 cut(s) 77, 180, 305, 683, 1789
HincII GTYRAC 1 cut(s) 2612
HindII GTYRAC 1 cut(s) 2612
HindIII AAGCTT 1 cut(s) 2162
HinfI GANTC 7 cut(s) 267, 533, 583, 739, 1196, 1519, 1661
HpaII CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1637, 2528
HphI GGTGA 5 cut(s) 241, 293, 756, 1170, 1790
Hpy166II GTNNAC 6 cut(s) 232, 261, 738, 1560, 1942, 2612
Hpy8I GTNNAC 6 cut(s) 232, 261, 738, 1560, 1942, 2612
Hpy99I CGWCG 5 cut(s) 71, 74, 77, 116, 978
HpyAV CCTTC 7 cut(s) 167, 584, 612, 771, 842, 848, 1424
HpyCH4III ACNGT 7 cut(s) 41, 291, 499, 1479, 1723, 1773, 2382
HpyCH4IV ACGT 4 cut(s) 567, 838, 1835, 1951
HpySE526I ACGT 4 cut(s) 567, 838, 1835, 1951
Hsp92I GRCGYC 2 cut(s) 180, 683
HspAI GCGC 5 cut(s) 77, 180, 305, 683, 1789
KasI GGCGCC 2 cut(s) 179, 682
KroI GCCGGC 1 cut(s) 274
KroNI GCCGGC 1 cut(s) 276
Ksp22I TGATCA 1 cut(s) 2400
LmnI GCTCC 6 cut(s) 34, 857, 964, 1714, 1744, 2572
LweI GCATC 6 cut(s) 80, 210, 685, 1004, 2176, 2556
MaeI CTAG 6 cut(s) 209, 264, 609, 689, 1215, 1287
MaeII ACGT 4 cut(s) 567, 838, 1835, 1951
MaeIII GTNAC 8 cut(s) 95, 217, 493, 811, 913, 1391, 2284, 2305
MbiI CCGCTC 1 cut(s) 636
MfeI CAATTG 1 cut(s) 2396
MflI RGATCY 3 cut(s) 1701, 2132, 2209
MhlI GDGCHC 4 cut(s) 369, 544, 634, 2353
MlsI TGGCCA 1 cut(s) 459
MluI ACGCGT 1 cut(s) 2545
MluNI TGGCCA 1 cut(s) 459
Mly113I GGCGCC 2 cut(s) 180, 683
MlyI GAGTC 2 cut(s) 592, 733
Mox20I TGGCCA 1 cut(s) 459
Mph1103I ATGCAT 3 cut(s) 717, 2009, 2118
MroNI GCCGGC 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 1652
MscI TGGCCA 1 cut(s) 459
MseI TTAA 6 cut(s) 1130, 1641, 1746, 1784, 1946, 2204
MslI CAYNNNNRTG 5 cut(s) 720, 2111, 2180, 2358, 2564
Msp20I TGGCCA 1 cut(s) 459
MspA1I CMGCKG 4 cut(s) 504, 884, 1732, 2506
MspI CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1637, 2528
MunI CAATTG 1 cut(s) 2396
Mva1269I GAATGC 1 cut(s) 2009
MvaI CCWGG 6 cut(s) 444, 538, 1058, 1564, 2277, 2390
MvnI CGCG 3 cut(s) 77, 88, 2547
NaeI GCCGGC 1 cut(s) 276
NarI GGCGCC 2 cut(s) 180, 683
NciI CCSGG 5 cut(s) 26, 81, 198, 679, 750
NcoI CCATGG 2 cut(s) 14, 1242
NgoMIV GCCGGC 1 cut(s) 274
NheI GCTAGC 1 cut(s) 208
NlaIV GGNNCC 5 cut(s) 30, 136, 160, 181, 684
NmuCI GTSAC 6 cut(s) 95, 217, 913, 1391, 2284, 2305
NsiI ATGCAT 3 cut(s) 717, 2009, 2118
NspI RCATGY 3 cut(s) 649, 782, 812
NspV TTCGAA 2 cut(s) 8, 1497
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 1 cut(s) 2353
PasI CCCWGGG 1 cut(s) 2389
PceI AGGCCT 1 cut(s) 601
PciI ACATGT 1 cut(s) 808
PcsI WCGNNNNNNNCGW 1 cut(s) 1665
PctI GAATGC 1 cut(s) 2009
PdiI GCCGGC 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 1652
PfeI GAWTC 5 cut(s) 267, 533, 1196, 1519, 1661
PflMI CCANNNNNTGG 2 cut(s) 258, 466
PleI GAGTC 2 cut(s) 591, 733
PluTI GGCGCC 2 cut(s) 183, 686
PpsI GAGTC 2 cut(s) 591, 733
Ppu21I YACGTR 2 cut(s) 568, 1952
PpuMI RGGWCCY 1 cut(s) 440
PscI ACATGT 1 cut(s) 808
PshAI GACNNNNGTC 1 cut(s) 1480
PsiI TTATAA 1 cut(s) 2345
Psp124BI GAGCTC 1 cut(s) 2353
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 6 cut(s) 442, 536, 1056, 1562, 2275, 2388
PspFI CCCAGC 2 cut(s) 362, 2189
PspGI CCWGG 6 cut(s) 442, 536, 1056, 1562, 2275, 2388
PspN4I GGNNCC 5 cut(s) 30, 136, 160, 181, 684
PspPI GGNCC 7 cut(s) 83, 134, 356, 440, 446, 2273, 2294
PspPPI RGGWCCY 1 cut(s) 440
PstI CTGCAG 2 cut(s) 322, 1825
PsuI RGATCY 3 cut(s) 1701, 2132, 2209
PvuII CAGCTG 1 cut(s) 1732
RsaI GTAC 3 cut(s) 23, 43, 1838
RsaNI GTAC 3 cut(s) 22, 42, 1837
RseI CAYNNNNRTG 5 cut(s) 720, 2111, 2180, 2358, 2564
SacI GAGCTC 1 cut(s) 2353
SalI GTCGAC 1 cut(s) 2610
SaqAI TTAA 6 cut(s) 1130, 1641, 1746, 1784, 1946, 2204
Sau96I GGNCC 7 cut(s) 83, 134, 356, 440, 446, 2273, 2294
SchI GAGTC 2 cut(s) 592, 733
SduI GDGCHC 4 cut(s) 369, 544, 634, 2353
SfaNI GCATC 6 cut(s) 80, 210, 685, 1004, 2176, 2556
SfcI CTRYAG 6 cut(s) 318, 843, 1449, 1821, 2145, 2229
SfoI GGCGCC 2 cut(s) 181, 684
Sfr274I CTCGAG 1 cut(s) 47
SfuI TTCGAA 2 cut(s) 8, 1497
SinI GGWCC 2 cut(s) 356, 440
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 5 cut(s) 720, 2111, 2180, 2358, 2564
SmlI CTYRAG 4 cut(s) 47, 1031, 1222, 1916
SmoI CTYRAG 4 cut(s) 47, 1031, 1222, 1916
SnaBI TACGTA 1 cut(s) 568
SseBI AGGCCT 1 cut(s) 601
SspDI GGCGCC 2 cut(s) 179, 682
SspMI CTAG 6 cut(s) 209, 264, 609, 689, 1215, 1287
SstI GAGCTC 1 cut(s) 2353
StuI AGGCCT 1 cut(s) 601
StyI CCWWGG 3 cut(s) 14, 1242, 1619
TaaI ACNGT 7 cut(s) 41, 291, 499, 1479, 1723, 1773, 2382
TaiI ACGT 4 cut(s) 570, 841, 1838, 1954
TaqI TCGA 6 cut(s) 8, 48, 1497, 1659, 1873, 2611
TauI GCSGC 8 cut(s) 119, 180, 185, 305, 507, 884, 995, 2593
TfiI GAWTC 5 cut(s) 267, 533, 1196, 1519, 1661
Tru1I TTAA 6 cut(s) 1130, 1641, 1746, 1784, 1946, 2204
Tru9I TTAA 6 cut(s) 1130, 1641, 1746, 1784, 1946, 2204
TscAI CASTG 5 cut(s) 327, 634, 734, 883, 2085
TseFI GTSAC 6 cut(s) 95, 217, 913, 1391, 2284, 2305
Tsp45I GTSAC 6 cut(s) 95, 217, 913, 1391, 2284, 2305
TspDTI ATGAA 8 cut(s) 1062, 1139, 1406, 1541, 1754, 1815, 2370, 2489
TspGWI ACGGA 4 cut(s) 389, 940, 1412, 2324
TspRI CASTG 5 cut(s) 327, 634, 734, 883, 2085
Van91I CCANNNNNTGG 2 cut(s) 258, 466
VpaK11BI GGWCC 2 cut(s) 356, 440
XapI RAATTY 3 cut(s) 1069, 1237, 2140
XceI RCATGY 3 cut(s) 649, 782, 812
XhoI CTCGAG 1 cut(s) 47
XmiI GTMKAC 1 cut(s) 2611
XmnI GAANNNNTTC 1 cut(s) 1652
XspI CTAG 6 cut(s) 209, 264, 609, 689, 1215, 1287
Zsp2I ATGCAT 3 cut(s) 717, 2009, 2118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.