MD11G1296400.v1.1

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0006473
Physical Location & Seq
Forward (+)
41407336 .. 41413456
6121 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1296400.v1.1.491

Sequence Viewer

Length: 2661 bp
ATGGGTTCGAAGCCATGGCTGTTCCCGGCTCCGACCTATCGTACTCTCGAGACCTTTTGGGATTGCGACGACGACGCCCCTGGTCCGCGCTGCAGTCACACCCTCACCGCCGTCGCCGCCACCAAGACCCACGGCCCCCGTCTCATCCTTTTCGGCGGTGCCACCGCCATTGAAGGCGGTGCCGCGTCCTCCGCCCCTGGCATCAGGTTAGCTGGGGTGACCAATTCGGTGCATTCCTATGATATTCTCACCAGGAAATGGACTAGAATGAGGCCTGCAGGTGAGCCACCCTCTCCGAGGGCAGCTCACGCAGCAGCTGCGGTGGGCACTATGGTTGTATTTCAGGGCGGTATAGGTCCTGCTGGGCATTCCACAGATGATCTCTACGTGCTGGACTTAGCCAACGATAAATTTAAATGGCACAGAGTGGTTGTACAAGGGCAAGGACCCGGGCCTCGCTACGGCCATGTAATGGACTTAGTTGCTCAAAGATATCTTGTTACCGTCAGCGGAAACGATGGAAAAAGAATTCATTCGGATGCTTGGGCTCTGGATACTGCTCAGAAACCTTATGCATGGGCTAGGCTGAACCCAGAAGGGGATAGACCGTCTGCTAGAATGTATGCAACAGCCAGTTCTCGCTCAGATGGTATGTTTTTGCTTTGTGGTGGAAGAGATATTTCTGGAACGCCACTAGCAGACGCTTATGGACTGCTTATGCATAGGAATGGTCAGTGGGAGTGGACCCTTGCACCTGGTGTATCCCCTTCACCAAGATATCAACATGCGGCAGTTTTTGTTGGTGCAAGATTACATGTCACAGGAGGTGCTCTTAGGGGAGGTCGTGGTATAGAAGGTGAAGCTGCTATTGCAGTATTGGACACTGCTGCTGGAGTTTGGTTGGACAGAAATGGGCTTGTGACTTCCTCACGGACAGGAAAGGGACAGAATGACCATGATCCTTCTTTGGAGCTCATGCGTCGTTCTCGCCATGCAGCTGCATCTCTTGGTGTTCGTATATATATTTATGGTGGTCTTAAAGGAGACATCCTGCTAGATGATTTCCTGGTTGCAGAAAATTCATCAGTTCAGTCTGAAGGTAATTCGCCTGTTGTAACGTCTGAGAGATCCCCAACGGTATCCAGTCCCAGAATGTTTCATGCTAACACAAGGACTTTTGGAACATCATCTTCTGATGGTGAAACTGAGAGTCCCTCGTCAACTGGCTTGAGCATGGACAAAAGTTCAATGGAGAAACTCCGGGAGGCTTCTGCTGCCGAAGCTGAGGCAGCTAGTGCTGTTTGGCAAGCTGTGCAATCAACATCTGCTACTCCTTCTGAAGAAACATCCGTTTCAGATGATAATTCACAAGTTGCAGAAACATTGTCTGATGGTAGTGACACCGAGGCAGATGTTCGTCTTCATCACAGAGCTGTTGTTGTTGCCAAAGAGGCTGTAGGCAACTTGGGTGGGATGGTAAGACAGTTGTCTTTGGATCAATTTGAAAATGAGAGCAGACGAACGATTCCAATGAACAATGACTTATCACATCCCAACAAAAAGTACACCAGGCAGAAGTCTCCACAAGGGCTACATAAGAAGGTTATATCTACATTGTTGAGGCCTCGAAACTGGAAACCCCCTGTCAATAGGAAGTTTTTCCTGGATTCTTATGAAGTGGGGGAACTTTGTTATGCTGCTGAGCAGATATTTATGCACGAGCAAACAGTTCTTCAGCTGAAAGCCCCAGTAAAAGTATTTGGCGATCTTCATGGACAGTTTGGTGATTTAATGCGCTTATTTGATGAATATGGATTTCCATCTGCTGCCGGAGACATAACGTATATCGACTACTTGTTTTTGGGGGATTATGTCGATCGAGGACAGCACAGCTTGGAGACCATAACTTTGCTCCTTGCACTGAAGATCGAGTATCCCGACAATGTTCACTTAATACGTGGAAACCATGAAGCTGCTGATATCAATGCACTCTTTGGTTTTCGTATTGAATGCATTGAGAGAATGGGTGAGAATGATGGAATCTGGGCATGGACTCGGTTCAATCAACTTTTCAACTATCTCCCACTTGCTGCTCTGATTGAAAAGAAAATTATCTGTATGCATGGAGGCATTGGGAGATCTATCAATTCTGTAGAACAGATAGAGAAGCTTGAAAGACCCATTACAATGGATGCTGGCTCTATAATTTTAATGGATCTTTTGTGGTCCGATCCCACAGAAAACGACAGTGTAGAGGGCTTGAGACCAAATGCCAGAGGGCCTGGTCTTGTCACCTTTGGGCCTGATCGTGTCGCAGAATTTTGTAAGAGAAACAAATTACAACTCATTGTAAGAGCTCATGAATGTGTCATGGATGGATTTGAAAGATTTGCCCAGGGACAGTTGATCACTCTTTTTTCTGCAACCAACTATTGCGGGACGGCAAACAATGCTGGTGCTATACTGGTCGTTGGCAGAGGTTTGGTTGTGGTTCCAAAATTAATCCATCCTTTGCCGCCTCCTCTTCAGTCACCGGAGACATCTCCCGAACGGGTCATAGACGACACATGGATGCAGGAGCTCAACATTCAAAGACCACCAACGCCTACTCGTGGTCGACCACAGCCTGATCTTGATCGAAACTCACTTGCATATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4 Metallophos STPPase_N

Protein Analysis

887

Amino Acids

96.76

Weight (kDa)

6.13

Isoelectric Point (pI)

39.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_FBX42 PF13415 23 - 188 1.1e-23 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 33 - 276 8.2e-38 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_HCF PF13854 72 - 348 3.7e-15 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 180 - 357 2.9e-11 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 203 - 277 2.8e-06 tRNA wybutosine-synthesizing protein 4, beta-propeller
Metallophos PF00149 583 - 790 8.5e-40 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 269
Acc36I ACCTGC 1 cut(s) 269
AccB1I GGYRCC 2 cut(s) 158, 179
AccB7I CCANNNNNTGG 2 cut(s) 258, 472
AccI GTMKAC 1 cut(s) 2617
AccII CGCG 2 cut(s) 88, 185
AclWI GGATC 5 cut(s) 953, 1122, 1503, 2223, 2225
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 4 cut(s) 410, 528, 1078, 2318
AcuI CTGAAG 5 cut(s) 1116, 1359, 1718, 1943, 2510
AcyI GRCGYC 1 cut(s) 75
AdeI CACNNNGTG 2 cut(s) 427, 758
AfaI GTAC 3 cut(s) 43, 435, 1565
AfiI CCNNNNNNNGG 6 cut(s) 258, 297, 461, 472, 598, 2612
AflIII ACRYGT 1 cut(s) 814
AjnI CCWGG 9 cut(s) 79, 196, 251, 754, 1065, 1568, 1662, 2281, 2394
Alw21I GWGCWC 4 cut(s) 832, 975, 2359, 2583
Alw26I GTCTC 8 cut(s) 44, 146, 1038, 1584, 1827, 1892, 2257, 2531
AlwI GGATC 5 cut(s) 953, 1122, 1503, 2223, 2225
AlwNI CAGNNNCTG 1 cut(s) 317
Ama87I CYCGRG 2 cut(s) 47, 449
AoxI GGCC 7 cut(s) 133, 272, 452, 463, 1622, 2279, 2300
ApoI RAATTY 4 cut(s) 410, 528, 1078, 2318
ArsI GACNNNNNNTTYG 2 cut(s) 1891, 1923
AseI ATTAAT 1 cut(s) 2501
Asp700I GAANNNNTTC 2 cut(s) 532, 1658
AspLEI GCGC 2 cut(s) 90, 1797
AspS9I GGNCC 9 cut(s) 83, 134, 356, 446, 452, 744, 2226, 2279, 2300
AsuC2I CCSGG 4 cut(s) 26, 450, 451, 1262
AsuII TTCGAA 1 cut(s) 8
AvaI CYCGRG 2 cut(s) 47, 449
AvaII GGWCC 5 cut(s) 83, 356, 446, 744, 2226
BaeGI GKGCMC 1 cut(s) 329
BanI GGYRCC 2 cut(s) 158, 179
BanII GRGCYC 4 cut(s) 550, 975, 2359, 2583
BauI CACGAG 2 cut(s) 1718, 2610
BbsI GAAGAC 1 cut(s) 1412
Bbv12I GWGCWC 4 cut(s) 832, 975, 2359, 2583
BbvCI CCTCAGC 1 cut(s) 1284
BccI CCATC 9 cut(s) 512, 641, 1190, 1385, 1468, 1828, 2030, 2369, 2514
BceAI ACGGC 4 cut(s) 95, 148, 478, 2457
BciT130I CCWGG 9 cut(s) 81, 198, 253, 756, 1067, 1570, 1664, 2283, 2396
BciVI GTATCC 4 cut(s) 547, 772, 1150, 1944
BclI TGATCA 1 cut(s) 2406
BcnI CCSGG 4 cut(s) 26, 450, 451, 1262
BcoDI GTCTC 8 cut(s) 44, 146, 1038, 1584, 1827, 1892, 2257, 2531
BfaI CTAG 6 cut(s) 264, 582, 615, 695, 1055, 1293
BfmI CTRYAG 4 cut(s) 91, 276, 1455, 2151
BfuAI ACCTGC 1 cut(s) 269
BfuI GTATCC 4 cut(s) 547, 772, 1150, 1944
BglI GCCNNNNNGGC 1 cut(s) 1451
BglII AGATCT 1 cut(s) 2138
BlpI GCTNAGC 1 cut(s) 1701
Bme18I GGWCC 5 cut(s) 83, 356, 446, 744, 2226
BmeT110I CYCGRG 2 cut(s) 47, 449
BmgT120I GGNCC 9 cut(s) 83, 134, 356, 446, 452, 744, 2226, 2279, 2300
BmiI GGNNCC 7 cut(s) 30, 136, 160, 181, 448, 746, 2493
BmrI ACTGGG 1 cut(s) 1742
BmsI GCATC 5 cut(s) 210, 529, 1010, 2182, 2562
BmuI ACTGGG 1 cut(s) 1742
BoxI GACNNNNGTC 1 cut(s) 1486
BpiI GAAGAC 1 cut(s) 1412
BplI GAGNNNNNCTC 6 cut(s) 275, 307, 289, 321, 1199, 1231
BpmI CTGGAG 1 cut(s) 912
Bpu10I CCTNAGC 1 cut(s) 1284
Bpu1102I GCTNAGC 1 cut(s) 1701
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 2 cut(s) 1249, 2281
BpuMI CCSGG 4 cut(s) 26, 450, 451, 1262
BsaAI YACGTR 2 cut(s) 388, 1958
BsaBI GATNNNNATC 2 cut(s) 1819, 2634
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 3 cut(s) 44, 1892, 2257
BsaJI CCNNGG 9 cut(s) 14, 79, 130, 196, 296, 449, 1404, 2394, 2395
BsaWI WCCGGW 1 cut(s) 2533
Bsc4I CCNNNNNNNGG 6 cut(s) 258, 297, 461, 472, 598, 2612
Bse1I ACTGG 6 cut(s) 633, 1143, 1228, 1637, 1748, 2469
Bse8I GATNNNNATC 2 cut(s) 1819, 2634
BseBI CCWGG 9 cut(s) 81, 198, 253, 756, 1067, 1570, 1664, 2283, 2396
BseDI CCNNGG 9 cut(s) 14, 79, 130, 196, 296, 449, 1404, 2394, 2395
BseJI GATNNNNATC 2 cut(s) 1819, 2634
BseLI CCNNNNNNNGG 6 cut(s) 258, 297, 461, 472, 598, 2612
BseMII CTCAG 6 cut(s) 575, 657, 1113, 1197, 1275, 1692
BseNI ACTGG 6 cut(s) 633, 1143, 1228, 1637, 1748, 2469
BseRI GAGGAG 1 cut(s) 2511
BseSI GKGCMC 1 cut(s) 329
BseYI CCCAGC 2 cut(s) 212, 362
Bsh1236I CGCG 2 cut(s) 88, 185
Bsh1285I CGRYCG 1 cut(s) 1879
BshFI GGCC 7 cut(s) 135, 274, 454, 465, 1624, 2281, 2302
BshNI GGYRCC 2 cut(s) 158, 179
BsiEI CGRYCG 1 cut(s) 1879
BsiHKAI GWGCWC 4 cut(s) 832, 975, 2359, 2583
BsiHKCI CYCGRG 2 cut(s) 47, 449
BsiSI CCGG 5 cut(s) 26, 450, 1261, 1830, 2534
BslFI GGGAC 5 cut(s) 957, 1131, 1197, 2412, 2452
BslI CCNNNNNNNGG 6 cut(s) 258, 297, 461, 472, 598, 2612
BsmAI GTCTC 8 cut(s) 44, 146, 1038, 1584, 1827, 1892, 2257, 2531
BsmBI CGTCTC 1 cut(s) 146
BsmFI GGGAC 5 cut(s) 957, 1131, 1197, 2412, 2452
BsmI GAATGC 3 cut(s) 232, 367, 2015
BsnI GGCC 7 cut(s) 135, 274, 454, 465, 1624, 2281, 2302
Bso31I GGTCTC 3 cut(s) 44, 1892, 2257
BsoBI CYCGRG 2 cut(s) 47, 449
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 6 cut(s) 329, 550, 832, 975, 2359, 2583
Bsp1407I TGTACA 1 cut(s) 433
Bsp1720I GCTNAGC 1 cut(s) 1701
Bsp19I CCATGG 1 cut(s) 14
BspANI GGCC 7 cut(s) 135, 274, 454, 465, 1624, 2281, 2302
BspCNI CTCAG 6 cut(s) 574, 656, 1114, 1198, 1276, 1693
BspFNI CGCG 2 cut(s) 88, 185
BspHI TCATGA 1 cut(s) 2359
BspLI GGNNCC 7 cut(s) 30, 136, 160, 181, 448, 746, 2493
BspMAI CTGCAG 2 cut(s) 95, 280
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 5 cut(s) 953, 1122, 1503, 2223, 2225
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 2 cut(s) 158, 179
BspTNI GGTCTC 3 cut(s) 44, 1892, 2257
BsrGI TGTACA 1 cut(s) 433
BsrI ACTGG 6 cut(s) 633, 1143, 1228, 1637, 1748, 2469
BssECI CCNNGG 9 cut(s) 14, 79, 130, 196, 296, 449, 1404, 2394, 2395
BssNI GRCGYC 1 cut(s) 75
BssSI CACGAG 2 cut(s) 1718, 2610
BssT1I CCWWGG 1 cut(s) 14
Bst2BI CACGAG 2 cut(s) 1718, 2610
Bst2UI CCWGG 9 cut(s) 81, 198, 253, 756, 1067, 1570, 1664, 2283, 2396
Bst4CI ACNGT 8 cut(s) 505, 609, 1138, 1485, 1729, 1779, 2249, 2403
Bst6I CTCTTC 2 cut(s) 667, 2529
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 4 cut(s) 317, 1295, 1312, 2450
BstAUI TGTACA 1 cut(s) 433
BstBAI YACGTR 2 cut(s) 388, 1958
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 276, 1308, 2197
BstDEI CTNAG 9 cut(s) 397, 478, 561, 643, 833, 1122, 1206, 1284, 1701
BstDSI CCRYGG 2 cut(s) 14, 130
BstEII GGTNACC 1 cut(s) 217
BstENI CCTNNNNNAGG 1 cut(s) 295
BstFNI CGCG 2 cut(s) 88, 185
BstHHI GCGC 2 cut(s) 90, 1797
BstMAI GTCTC 8 cut(s) 44, 146, 1038, 1584, 1827, 1892, 2257, 2531
BstMCI CGRYCG 1 cut(s) 1879
BstNI CCWGG 9 cut(s) 81, 198, 253, 756, 1067, 1570, 1664, 2283, 2396
BstNSI RCATGY 2 cut(s) 788, 818
BstPAI GACNNNNGTC 1 cut(s) 1486
BstPI GGTNACC 1 cut(s) 217
BstSFI CTRYAG 4 cut(s) 91, 276, 1455, 2151
BstSLI GKGCMC 1 cut(s) 329
BstUI CGCG 2 cut(s) 88, 185
BstV2I GAAGAC 1 cut(s) 1412
BstX2I RGATCY 3 cut(s) 1127, 2138, 2215
BstXI CCANNNNNNTGG 1 cut(s) 2188
BstYI RGATCY 3 cut(s) 1127, 2138, 2215
BsuI GTATCC 4 cut(s) 547, 772, 1150, 1944
BsuRI GGCC 7 cut(s) 135, 274, 454, 465, 1624, 2281, 2302
BtgI CCRYGG 2 cut(s) 14, 130
BtsI GCAGTG 1 cut(s) 882
BtsIMutI CAGTG 4 cut(s) 740, 882, 1919, 2254
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 3 cut(s) 276, 1308, 2197
CaiI CAGNNNCTG 1 cut(s) 317
CciI TCATGA 1 cut(s) 2359
CfoI GCGC 2 cut(s) 90, 1797
Cfr13I GGNCC 9 cut(s) 83, 134, 356, 446, 452, 744, 2226, 2279, 2300
Cfr9I CCCGGG 1 cut(s) 449
CseI GACGC 4 cut(s) 83, 174, 710, 968
CsiI ACCWGGT 1 cut(s) 754
Csp6I GTAC 3 cut(s) 42, 434, 1564
CspCI CAANNNNNGTGG 4 cut(s) 151, 186, 1450, 1485
CviQI GTAC 3 cut(s) 42, 434, 1564
DdeI CTNAG 9 cut(s) 397, 478, 561, 643, 833, 1122, 1206, 1284, 1701
DraI TTTAAA 1 cut(s) 415
DraIII CACNNNGTG 2 cut(s) 427, 758
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 2 cut(s) 667, 2529
EarI CTCTTC 2 cut(s) 667, 2529
EciI GGCGGA 1 cut(s) 181
Ecl136II GAGCTC 3 cut(s) 973, 2357, 2581
Eco130I CCWWGG 1 cut(s) 14
Eco147I AGGCCT 2 cut(s) 274, 1624
Eco24I GRGCYC 4 cut(s) 550, 975, 2359, 2583
Eco31I GGTCTC 3 cut(s) 44, 1892, 2257
Eco32I GATATC 3 cut(s) 494, 779, 1981
Eco47I GGWCC 5 cut(s) 83, 356, 446, 744, 2226
Eco53kI GAGCTC 3 cut(s) 973, 2357, 2581
Eco57I CTGAAG 5 cut(s) 1116, 1359, 1718, 1943, 2510
Eco88I CYCGRG 2 cut(s) 47, 449
Eco91I GGTNACC 1 cut(s) 217
EcoICRI GAGCTC 3 cut(s) 973, 2357, 2581
EcoNI CCTNNNNNAGG 1 cut(s) 295
EcoO109I RGGNCCY 3 cut(s) 356, 446, 2279
EcoO65I GGTNACC 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 528
EcoRII CCWGG 9 cut(s) 79, 196, 251, 754, 1065, 1568, 1662, 2281, 2394
EcoRV GATATC 3 cut(s) 494, 779, 1981
EcoT14I CCWWGG 1 cut(s) 14
EcoT22I ATGCAT 4 cut(s) 577, 723, 2015, 2124
EcoT38I GRGCYC 4 cut(s) 550, 975, 2359, 2583
ErhI CCWWGG 1 cut(s) 14
Esp3I CGTCTC 1 cut(s) 146
FaqI GGGAC 5 cut(s) 957, 1131, 1197, 2412, 2452
FauI CCCGC 1 cut(s) 2429
FbaI TGATCA 1 cut(s) 2406
FblI GTMKAC 1 cut(s) 2617
FriOI GRGCYC 4 cut(s) 550, 975, 2359, 2583
FspBI CTAG 6 cut(s) 264, 582, 615, 695, 1055, 1293
GlaI GCGC 2 cut(s) 89, 1796
GsaI CCCAGC 2 cut(s) 216, 366
GsuI CTGGAG 1 cut(s) 912
HaeIII GGCC 7 cut(s) 135, 274, 454, 465, 1624, 2281, 2302
HapII CCGG 5 cut(s) 26, 450, 1261, 1830, 2534
HgaI GACGC 4 cut(s) 83, 174, 710, 968
HhaI GCGC 2 cut(s) 90, 1797
Hin1I GRCGYC 1 cut(s) 75
Hin6I GCGC 2 cut(s) 88, 1795
HinP1I GCGC 2 cut(s) 88, 1795
HincII GTYRAC 2 cut(s) 1221, 2618
HindII GTYRAC 2 cut(s) 1221, 2618
HindIII AAGCTT 1 cut(s) 2168
HinfI GANTC 5 cut(s) 1210, 1525, 1667, 2040, 2053
HpaII CCGG 5 cut(s) 26, 450, 1261, 1830, 2534
Hpy166II GTNNAC 5 cut(s) 744, 1221, 1566, 1948, 2618
Hpy188III TCNNGA 8 cut(s) 47, 49, 551, 684, 1937, 2360, 2546, 2633
Hpy8I GTNNAC 5 cut(s) 744, 1221, 1566, 1948, 2618
Hpy99I CGWCG 5 cut(s) 71, 74, 77, 116, 984
HpyAV CCTTC 8 cut(s) 167, 590, 777, 848, 972, 1091, 1344, 1594
HpyCH4III ACNGT 8 cut(s) 505, 609, 1138, 1485, 1729, 1779, 2249, 2403
HpyCH4IV ACGT 4 cut(s) 387, 1118, 1841, 1957
HpyF3I CTNAG 9 cut(s) 397, 478, 561, 643, 833, 1122, 1206, 1284, 1701
HpySE526I ACGT 4 cut(s) 387, 1118, 1841, 1957
Hsp92I GRCGYC 1 cut(s) 75
HspAI GCGC 2 cut(s) 88, 1795
Ksp22I TGATCA 1 cut(s) 2406
LmnI GCTCC 4 cut(s) 34, 970, 1917, 2578
LweI GCATC 5 cut(s) 210, 529, 1010, 2182, 2562
MabI ACCWGGT 1 cut(s) 754
MaeI CTAG 6 cut(s) 264, 582, 615, 695, 1055, 1293
MaeII ACGT 4 cut(s) 387, 1118, 1841, 1957
MaeIII GTNAC 9 cut(s) 95, 217, 499, 817, 919, 1114, 1397, 2290, 2529
MboII GAAGA 8 cut(s) 684, 1182, 1352, 1412, 1724, 1760, 1936, 2516
MflI RGATCY 3 cut(s) 1127, 2138, 2215
MhlI GDGCHC 6 cut(s) 329, 550, 832, 975, 2359, 2583
MlyI GAGTC 2 cut(s) 1219, 2047
MmeI TCCRAC 2 cut(s) 56, 882
Mph1103I ATGCAT 4 cut(s) 577, 723, 2015, 2124
MroXI GAANNNNTTC 2 cut(s) 532, 1658
MseI TTAA 6 cut(s) 414, 1038, 1790, 1952, 2210, 2501
MslI CAYNNNNRTG 6 cut(s) 237, 537, 726, 2186, 2364, 2570
MspA1I CMGCKG 4 cut(s) 317, 510, 998, 1738
MspI CCGG 5 cut(s) 26, 450, 1261, 1830, 2534
Mva1269I GAATGC 3 cut(s) 232, 367, 2015
MvaI CCWGG 9 cut(s) 81, 198, 253, 756, 1067, 1570, 1664, 2283, 2396
MvnI CGCG 2 cut(s) 88, 185
NciI CCSGG 4 cut(s) 26, 450, 451, 1262
NcoI CCATGG 1 cut(s) 14
NlaIV GGNNCC 7 cut(s) 30, 136, 160, 181, 448, 746, 2493
NmuCI GTSAC 7 cut(s) 95, 217, 817, 919, 1397, 2290, 2529
NsiI ATGCAT 4 cut(s) 577, 723, 2015, 2124
NspI RCATGY 2 cut(s) 788, 818
NspV TTCGAA 1 cut(s) 8
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 1 cut(s) 2359
PaqCI CACCTGC 1 cut(s) 269
PasI CCCWGGG 1 cut(s) 2395
PceI AGGCCT 2 cut(s) 274, 1624
PciI ACATGT 1 cut(s) 814
PcsI WCGNNNNNNNCGW 1 cut(s) 1935
PctI GAATGC 3 cut(s) 232, 367, 2015
PdmI GAANNNNTTC 2 cut(s) 532, 1658
PfeI GAWTC 3 cut(s) 1525, 1667, 2040
PflMI CCANNNNNTGG 2 cut(s) 258, 472
PfoI TCCNGGA 2 cut(s) 1260, 1662
Ple19I CGATCG 1 cut(s) 1879
PleI GAGTC 2 cut(s) 1218, 2047
PpsI GAGTC 2 cut(s) 1218, 2047
Ppu21I YACGTR 2 cut(s) 388, 1958
PpuMI RGGWCCY 2 cut(s) 356, 446
PscI ACATGT 1 cut(s) 814
PshAI GACNNNNGTC 1 cut(s) 1486
PshBI ATTAAT 1 cut(s) 2501
Psp124BI GAGCTC 3 cut(s) 975, 2359, 2583
Psp5II RGGWCCY 2 cut(s) 356, 446
Psp6I CCWGG 9 cut(s) 79, 196, 251, 754, 1065, 1568, 1662, 2281, 2394
PspEI GGTNACC 1 cut(s) 217
PspFI CCCAGC 2 cut(s) 212, 362
PspGI CCWGG 9 cut(s) 79, 196, 251, 754, 1065, 1568, 1662, 2281, 2394
PspN4I GGNNCC 7 cut(s) 30, 136, 160, 181, 448, 746, 2493
PspPI GGNCC 9 cut(s) 83, 134, 356, 446, 452, 744, 2226, 2279, 2300
PspPPI RGGWCCY 2 cut(s) 356, 446
PstI CTGCAG 2 cut(s) 95, 280
PstNI CAGNNNCTG 1 cut(s) 317
PsuI RGATCY 3 cut(s) 1127, 2138, 2215
PvuI CGATCG 1 cut(s) 1879
PvuII CAGCTG 3 cut(s) 317, 998, 1738
RsaI GTAC 3 cut(s) 43, 435, 1565
RsaNI GTAC 3 cut(s) 42, 434, 1564
RseI CAYNNNNRTG 6 cut(s) 237, 537, 726, 2186, 2364, 2570
SacI GAGCTC 3 cut(s) 975, 2359, 2583
SalI GTCGAC 1 cut(s) 2616
SaqAI TTAA 6 cut(s) 414, 1038, 1790, 1952, 2210, 2501
Sau96I GGNCC 9 cut(s) 83, 134, 356, 446, 452, 744, 2226, 2279, 2300
SbfI CCTGCAGG 1 cut(s) 280
SchI GAGTC 2 cut(s) 1219, 2047
SdaI CCTGCAGG 1 cut(s) 280
SduI GDGCHC 6 cut(s) 329, 550, 832, 975, 2359, 2583
SexAI ACCWGGT 1 cut(s) 754
SfaNI GCATC 5 cut(s) 210, 529, 1010, 2182, 2562
SfcI CTRYAG 4 cut(s) 91, 276, 1455, 2151
Sfr274I CTCGAG 1 cut(s) 47
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 5 cut(s) 83, 356, 446, 744, 2226
SlaI CTCGAG 1 cut(s) 47
SmaI CCCGGG 1 cut(s) 451
SmiI ATTTAAAT 1 cut(s) 415
SmiMI CAYNNNNRTG 6 cut(s) 237, 537, 726, 2186, 2364, 2570
SmlI CTYRAG 3 cut(s) 47, 1228, 2260
SmoI CTYRAG 3 cut(s) 47, 1228, 2260
Sse8387I CCTGCAGG 1 cut(s) 280
SseBI AGGCCT 2 cut(s) 274, 1624
SspMI CTAG 6 cut(s) 264, 582, 615, 695, 1055, 1293
SstI GAGCTC 3 cut(s) 975, 2359, 2583
StuI AGGCCT 2 cut(s) 274, 1624
StyI CCWWGG 1 cut(s) 14
SwaI ATTTAAAT 1 cut(s) 415
TaaI ACNGT 8 cut(s) 505, 609, 1138, 1485, 1729, 1779, 2249, 2403
TaiI ACGT 4 cut(s) 390, 1121, 1844, 1960
TaqI TCGA 9 cut(s) 8, 48, 1627, 1848, 1875, 1879, 1929, 2617, 2638
TatI WGTACW 2 cut(s) 433, 1563
TauI GCSGC 4 cut(s) 119, 185, 791, 2518
TfiI GAWTC 3 cut(s) 1525, 1667, 2040
Tru1I TTAA 6 cut(s) 414, 1038, 1790, 1952, 2210, 2501
Tru9I TTAA 6 cut(s) 414, 1038, 1790, 1952, 2210, 2501
TscAI CASTG 4 cut(s) 740, 889, 1926, 2254
TseFI GTSAC 7 cut(s) 95, 217, 817, 919, 1397, 2290, 2529
Tsp45I GTSAC 7 cut(s) 95, 217, 817, 919, 1397, 2290, 2529
TspGWI ACGGA 2 cut(s) 946, 1339
TspMI CCCGGG 1 cut(s) 449
TspRI CASTG 4 cut(s) 740, 889, 1926, 2254
Van91I CCANNNNNTGG 2 cut(s) 258, 472
VpaK11BI GGWCC 5 cut(s) 83, 356, 446, 744, 2226
VspI ATTAAT 1 cut(s) 2501
XagI CCTNNNNNAGG 1 cut(s) 295
XapI RAATTY 4 cut(s) 410, 528, 1078, 2318
XceI RCATGY 2 cut(s) 788, 818
XhoI CTCGAG 1 cut(s) 47
XmaI CCCGGG 1 cut(s) 449
XmiI GTMKAC 1 cut(s) 2617
XmnI GAANNNNTTC 2 cut(s) 532, 1658
XspI CTAG 6 cut(s) 264, 582, 615, 695, 1055, 1293
Zsp2I ATGCAT 4 cut(s) 577, 723, 2015, 2124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.