MD03G1274100.v1.1

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006473
Physical Location & Seq
Forward (+)
35710860 .. 35716942
6083 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1274100.v1.1.491

Sequence Viewer

Length: 2655 bp
ATGGGTTCGAAGCCATGGCTGTACCCGGCGCCGACCTATCGTACTCTCGAGACCTATTGGGATTGCGACGACGACGCCCCTGGTCCCCGCTGCGCTCACACCCTCACCGCCGTCGCCGCCACTAAGACCCATGGCCCCCGTCTCATCCTCTTCGGCGGTGCCACCGCCATTGAAGGCGGCGCTGCCTCCGCCCCTGGCATCAGGTTAGCTGGGGTGACCAATTCGGTGCATTCGTACGATGTTCTCACCAGGAAATGGACTAGAATGAGGCCTGCAGGTGAGCCACCGTCTCCGAGGGCAGCTCACGCGGCGGCTGCGGTGGGCACTATGGTTGTATTTCAGGGTGGTATAGGTCCTGCTGGGCATTCCACAGATGATCTATACGTGCTGGACTTGACCAACGATAAGTTTAAATGGCACAGAGTGGTTGTACAAGGACAAGGTCCTGGGCCTCGCTACGGCCATGTAATGGACTTAGTTGCTCAAAGATATCTTGTTACTGTCAGTGGAAACGATGGAAAAAGAGTCCTTTCTGATGCCTGGGCTCTGGATACTGCTCAGAAGCCTTATGCATGGGCTAGGCTGAGCCCAGAAGGTGATAGGCCTTCTGCTAGAATGTATGCAACAGCCAGTGCCCGCTCAGATGGTATGTTTTTGCTTTGTGGTGGAAGAGATATTTCTGGAGCGCCACTAGCAGACGCTTATGGACTGCTTATGCATAGAAATGGTCAGTGGGAGTGGACCCTTGCACCTGGAGTATCACCTTCACCAAGGTATCAACATGCGGCGGTTTTTGTTGGGGCAAGATTACATGTTACAGGAGGTACTCTTAGGGGAGGCCGTTGTATAGAAGGCGAAGCAGCTGTTGCAGTATTGGACACTGCTGCTGGAGTTTGGTTAGACAGAAATGGGCTTGTGACTTCCTCACGCACAGGAAAGGGACAGAATGATTATGATCCTCCTTTGGAGCTCATGCGTCGTTGCCGCCATGCAGCTGCATCTCTTGGTGTTCGTATATACATTTATGGTGGTCTTAAGGGAGACATCCTGTTAGATGACTTCCTAGTTGCAGAAAATTCATTATTTCAGTCTGAAGGTAATTCGCCTGTTTTAACTTCTGAGAGACCCCCAACTGTATCAAGTCCCAGAATGTTTCATGCCAACACAAGTACTTTTGGAACATCATCTGATGGTGAACATGAGAGTCCCTCATCAAGTCGCTCAAGCATGGACAAAAGTTCAATGGAGAAACTCCGGGAGGCTTCTGCTGCCGAAGCTGAGGCAGCTAGTGCTGTTTGGCAAGCTGTGCAGTCAACATCTGCTACTCCTGCTGAAGAAACATCTGTTTCAGATGATAACTCGCAAGTTGCAGAAACATTGTCGGATGGTAGTGACACTGAGGCTGATGTTCGCCTTCATCACAGAGCTGTTGTTGTTGCCAAAGAGGCTGTAGGTAACTTGGGTGGGATGGTAAGACAGTTGTCTTTGGACCAATTTGAAAATGAAAGCAGACGAATGATTCCAATGAATAATGACTTATCATATCCCAACAAAAAGTTCACCAGGCAGAAGTCTCCACAAGGGCTACATAAAAAGGTTATATCTACATTGTTGAGGCCTCGAAACTGGAAAGCCCCTGTCAATAGGAAGTTTTTCATGGATTCTTATGAAGTGGGAGAACTTTGTTATGCTGCTGAGCAGATCTTTATGCAGGAGCAAACAGTTCTTCAGCTGAAAGCCCCTGTTAAAGTATTTGGCGATCTTCATGGACAGTTTGGTGATTTAATGCGCTTATTTGATGAATATGGATTTCCATCTGCTGCCGGAGACATAACGTATATCGACTACTTGTTTTTGGGGGATTATGTCGATCGAGGACAGCACAGCTTGGAGACCATAACTTTGCTCCTTGCACTGAAGATTGAGCATCCTGACAATGTTCACTTAATACGTGGAAACCATGAAGCTGCTGATATAAATGCACTCTTTGGTTTTCGTATTGAATGCATTGAGAGAATGGGCGAGAATGATGGAATATGGGCATGGACTCGATTCAATCAACTTTTCAACTATCTCCCACTTGCTGCTCTAATTGAAAAGAAAATTATCTGTATGCATGGAGGCATTGGGAGATCCATCAATTCTTTAGAACAGATAGAGAAGCTTGAAAGACCCATTACAATGGATGCTGGGTCTATAGTTTTAATGGATCTTTTGTGGTCTGATCCCACAGAAAACGATAGTGTAGAGGGCTTGAGACCAAATGCCAGAGGGCCTGGTCTTGTCACCTTTGGGCCTGATCGTGTCACGGAATTCTGTAAAAGAAACAAATTACAACTCATTATACGAGCTCATGAATGCGTCATGGATGGATTTGAAAGATTTGCCCAGGGACAGTTGATCACTCTTTTCTCTGCAACCAACTATTGCGGGACGGCAAACAATGCTGGTGCTATACTGGTTGTTGGCAGAGGTTTGGTTGTGGTTCCGAAATTAATCCATCCTTTGCCACCTCCTCTTCAGTCACCGGAGACATCTCCTGAACGGGTCATAGACGACACATGGATGCAGGAGCTCAACATTCAAAGACCACCAACACCTACTCGTGGCCGACCACAGCCTGATCTTGATCGAAACTCACTTGCATATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4 Metallophos STPPase_N

Protein Analysis

885

Amino Acids

96.77

Weight (kDa)

6.08

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 21 - 222 3.9e-32 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 22 - 187 4.6e-24 FBX42, beta-propeller domain
Kelch_HCF PF13854 71 - 347 3.9e-15 Host cell factor, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 73 - 351 2.3e-18 Attractin/LZTR1 beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 191 - 299 2.6e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 191 - 355 1.3e-09 FBX42, beta-propeller domain
Beta-prop_TYW4 PF13418 202 - 280 3e-06 tRNA wybutosine-synthesizing protein 4, beta-propeller
Kelch_KLHDC2_KLHL20_DRC7 PF24681 242 - 359 2.9e-06 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Metallophos PF00149 581 - 788 3.7e-39 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 266
Acc36I ACCTGC 1 cut(s) 266
AccB1I GGYRCC 2 cut(s) 28, 158
AccB7I CCANNNNNTGG 2 cut(s) 255, 469
AccBSI CCGCTC 1 cut(s) 639
AccII CGCG 1 cut(s) 308
AclWI GGATC 4 cut(s) 950, 2127, 2217, 2219
AcoI YGGCCR 2 cut(s) 460, 2608
AcsI RAATTY 2 cut(s) 1075, 2312
AcuI CTGAAG 5 cut(s) 1113, 1353, 1712, 1937, 2504
AcyI GRCGYC 2 cut(s) 29, 75
AdeI CACNNNGTG 1 cut(s) 424
AfaI GTAC 6 cut(s) 23, 43, 236, 432, 826, 1171
AfiI CCNNNNNNNGG 4 cut(s) 255, 458, 469, 2606
AflII CTTAAG 1 cut(s) 1034
AflIII ACRYGT 1 cut(s) 811
AjnI CCWGG 9 cut(s) 79, 193, 248, 445, 539, 751, 1562, 2275, 2388
AjuI GAANNNNNNNTTGG 2 cut(s) 1541, 1573
Alw21I GWGCWC 3 cut(s) 972, 2353, 2577
AlwI GGATC 4 cut(s) 950, 2127, 2217, 2219
Ama87I CYCGRG 1 cut(s) 47
ApoI RAATTY 2 cut(s) 1075, 2312
ArsI GACNNNNNNTTYG 2 cut(s) 1885, 1917
AseI ATTAAT 1 cut(s) 2495
Asp700I GAANNNNTTC 1 cut(s) 1652
AspLEI GCGC 5 cut(s) 31, 95, 182, 688, 1791
AspS9I GGNCC 9 cut(s) 83, 134, 353, 443, 449, 741, 1489, 2273, 2294
AsuC2I CCSGG 2 cut(s) 26, 1256
AsuII TTCGAA 1 cut(s) 8
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 5 cut(s) 83, 353, 443, 741, 1489
BaeGI GKGCMC 2 cut(s) 326, 637
BaeI ACNNNNGTAYC 2 cut(s) 758, 791
BanI GGYRCC 2 cut(s) 28, 158
BanII GRGCYC 5 cut(s) 547, 590, 972, 2353, 2577
BauI CACGAG 1 cut(s) 2604
Bbv12I GWGCWC 3 cut(s) 972, 2353, 2577
BbvCI CCTCAGC 1 cut(s) 1278
BceAI ACGGC 4 cut(s) 95, 475, 825, 2451
BciT130I CCWGG 9 cut(s) 81, 195, 250, 447, 541, 753, 1564, 2277, 2390
BciVI GTATCC 1 cut(s) 544
BclI TGATCA 1 cut(s) 2400
BcnI CCSGG 2 cut(s) 26, 1256
BfaI CTAG 6 cut(s) 261, 579, 612, 692, 1064, 1287
BfmI CTRYAG 3 cut(s) 273, 1449, 2196
BfoI RGCGCY 3 cut(s) 32, 183, 689
BfrI CTTAAG 1 cut(s) 1034
BfuAI ACCTGC 1 cut(s) 266
BfuI GTATCC 1 cut(s) 544
BglI GCCNNNNNGGC 1 cut(s) 1445
BglII AGATCT 1 cut(s) 1701
BlpI GCTNAGC 2 cut(s) 584, 1695
BmcAI AGTACT 1 cut(s) 1171
Bme18I GGWCC 5 cut(s) 83, 353, 443, 741, 1489
BmeT110I CYCGRG 1 cut(s) 47
BmgT120I GGNCC 9 cut(s) 83, 134, 353, 443, 449, 741, 1489, 2273, 2294
BmiI GGNNCC 6 cut(s) 30, 85, 136, 160, 743, 2487
BmsI GCATC 6 cut(s) 207, 526, 1007, 1936, 2176, 2556
BoxI GACNNNNGTC 1 cut(s) 1480
BplI GAGNNNNNCTC 4 cut(s) 286, 318, 1193, 1225
BpmI CTGGAG 3 cut(s) 702, 774, 909
Bpu10I CCTNAGC 1 cut(s) 1278
Bpu1102I GCTNAGC 2 cut(s) 584, 1695
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 2 cut(s) 1207, 2275
BpuMI CCSGG 2 cut(s) 26, 1256
BsaAI YACGTR 2 cut(s) 385, 1952
BsaBI GATNNNNATC 3 cut(s) 954, 1813, 2628
BsaHI GRCGYC 2 cut(s) 29, 75
BsaI GGTCTC 4 cut(s) 44, 1117, 1886, 2251
BsaWI WCCGGW 1 cut(s) 2527
Bsc4I CCNNNNNNNGG 4 cut(s) 255, 458, 469, 2606
Bse1I ACTGG 3 cut(s) 630, 1631, 2463
Bse8I GATNNNNATC 3 cut(s) 954, 1813, 2628
BseBI CCWGG 9 cut(s) 81, 195, 250, 447, 541, 753, 1564, 2277, 2390
BseGI GGATG 9 cut(s) 144, 1044, 1390, 1473, 1927, 2191, 2374, 2500, 2571
BseJI GATNNNNATC 3 cut(s) 954, 1813, 2628
BseLI CCNNNNNNNGG 4 cut(s) 255, 458, 469, 2606
BseMII CTCAG 7 cut(s) 572, 575, 654, 1110, 1269, 1389, 1686
BseNI ACTGG 3 cut(s) 630, 1631, 2463
BseRI GAGGAG 1 cut(s) 2505
BseSI GKGCMC 2 cut(s) 326, 637
BseYI CCCAGC 3 cut(s) 209, 359, 2189
BsgI GTGCAG 1 cut(s) 1328
Bsh1236I CGCG 1 cut(s) 308
Bsh1285I CGRYCG 1 cut(s) 1873
BshNI GGYRCC 2 cut(s) 28, 158
BsiEI CGRYCG 1 cut(s) 1873
BsiHKAI GWGCWC 3 cut(s) 972, 2353, 2577
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 4 cut(s) 26, 1255, 1824, 2528
BsiWI CGTACG 1 cut(s) 234
BslFI GGGAC 6 cut(s) 69, 954, 1128, 1191, 2406, 2446
BslI CCNNNNNNNGG 4 cut(s) 255, 458, 469, 2606
BsmBI CGTCTC 2 cut(s) 146, 294
BsmFI GGGAC 6 cut(s) 69, 954, 1128, 1191, 2406, 2446
BsmI GAATGC 4 cut(s) 229, 364, 2009, 2363
Bso31I GGTCTC 4 cut(s) 44, 1117, 1886, 2251
BsoBI CYCGRG 1 cut(s) 47
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 7 cut(s) 326, 547, 590, 637, 972, 2353, 2577
Bsp1407I TGTACA 1 cut(s) 430
Bsp1720I GCTNAGC 2 cut(s) 584, 1695
Bsp19I CCATGG 2 cut(s) 14, 130
BspCNI CTCAG 7 cut(s) 571, 576, 653, 1111, 1270, 1390, 1687
BspFNI CGCG 1 cut(s) 308
BspHI TCATGA 1 cut(s) 2353
BspLI GGNNCC 6 cut(s) 30, 85, 136, 160, 743, 2487
BspMAI CTGCAG 1 cut(s) 277
BspMI ACCTGC 1 cut(s) 266
BspPI GGATC 4 cut(s) 950, 2127, 2217, 2219
BspT104I TTCGAA 1 cut(s) 8
BspT107I GGYRCC 2 cut(s) 28, 158
BspTI CTTAAG 1 cut(s) 1034
BspTNI GGTCTC 4 cut(s) 44, 1117, 1886, 2251
BsrBI CCGCTC 1 cut(s) 639
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 3 cut(s) 630, 1631, 2463
BssNI GRCGYC 2 cut(s) 29, 75
BssSI CACGAG 1 cut(s) 2604
BssT1I CCWWGG 3 cut(s) 14, 130, 770
Bst2BI CACGAG 1 cut(s) 2604
Bst2UI CCWGG 9 cut(s) 81, 195, 250, 447, 541, 753, 1564, 2277, 2390
Bst4CI ACNGT 7 cut(s) 288, 502, 1135, 1479, 1723, 1773, 2397
Bst6I CTCTTC 3 cut(s) 155, 664, 2523
BstACI GRCGYC 2 cut(s) 29, 75
BstAFI CTTAAG 1 cut(s) 1034
BstAPI GCANNNNNTGC 4 cut(s) 866, 1289, 1306, 2444
BstAUI TGTACA 1 cut(s) 430
BstBAI YACGTR 2 cut(s) 385, 1952
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 273, 637, 1302
BstDSI CCRYGG 2 cut(s) 14, 130
BstEII GGTNACC 1 cut(s) 214
BstF5I GGATG 9 cut(s) 144, 1044, 1390, 1473, 1927, 2191, 2374, 2500, 2571
BstFNI CGCG 1 cut(s) 308
BstH2I RGCGCY 3 cut(s) 32, 183, 689
BstHHI GCGC 5 cut(s) 31, 95, 182, 688, 1791
BstMCI CGRYCG 1 cut(s) 1873
BstNI CCWGG 9 cut(s) 81, 195, 250, 447, 541, 753, 1564, 2277, 2390
BstNSI RCATGY 2 cut(s) 785, 815
BstPAI GACNNNNGTC 1 cut(s) 1480
BstPI GGTNACC 1 cut(s) 214
BstSFI CTRYAG 3 cut(s) 273, 1449, 2196
BstSLI GKGCMC 2 cut(s) 326, 637
BstUI CGCG 1 cut(s) 308
BstX2I RGATCY 3 cut(s) 1701, 2132, 2209
BstXI CCANNNNNNTGG 1 cut(s) 2182
BstYI RGATCY 3 cut(s) 1701, 2132, 2209
BsuI GTATCC 1 cut(s) 544
BtgI CCRYGG 2 cut(s) 14, 130
BtsCI GGATG 9 cut(s) 144, 1044, 1390, 1473, 1927, 2191, 2374, 2500, 2571
BtsI GCAGTG 1 cut(s) 879
BtsIMutI CAGTG 6 cut(s) 511, 637, 737, 879, 1395, 1913
BveI ACCTGC 1 cut(s) 266
Cac8I GCNNGC 3 cut(s) 273, 637, 1302
CciI TCATGA 1 cut(s) 2353
CfoI GCGC 5 cut(s) 31, 95, 182, 688, 1791
Cfr13I GGNCC 9 cut(s) 83, 134, 353, 443, 449, 741, 1489, 2273, 2294
CseI GACGC 4 cut(s) 83, 707, 965, 2350
Csp6I GTAC 6 cut(s) 22, 42, 235, 431, 825, 1170
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviQI GTAC 6 cut(s) 22, 42, 235, 431, 825, 1170
DinI GGCGCC 1 cut(s) 30
DraI TTTAAA 1 cut(s) 412
DraIII CACNNNGTG 1 cut(s) 424
EaeI YGGCCR 2 cut(s) 460, 2608
Eam1104I CTCTTC 3 cut(s) 155, 664, 2523
EarI CTCTTC 3 cut(s) 155, 664, 2523
EciI GGCGGA 1 cut(s) 178
Ecl136II GAGCTC 3 cut(s) 970, 2351, 2575
Eco130I CCWWGG 3 cut(s) 14, 130, 770
Eco147I AGGCCT 3 cut(s) 271, 604, 1618
Eco24I GRGCYC 5 cut(s) 547, 590, 972, 2353, 2577
Eco31I GGTCTC 4 cut(s) 44, 1117, 1886, 2251
Eco32I GATATC 1 cut(s) 491
Eco47I GGWCC 5 cut(s) 83, 353, 443, 741, 1489
Eco53kI GAGCTC 3 cut(s) 970, 2351, 2575
Eco57I CTGAAG 5 cut(s) 1113, 1353, 1712, 1937, 2504
Eco88I CYCGRG 1 cut(s) 47
Eco91I GGTNACC 1 cut(s) 214
EcoICRI GAGCTC 3 cut(s) 970, 2351, 2575
EcoO109I RGGNCCY 3 cut(s) 353, 443, 2273
EcoO65I GGTNACC 1 cut(s) 214
EcoRI GAATTC 1 cut(s) 2312
EcoRII CCWGG 9 cut(s) 79, 193, 248, 445, 539, 751, 1562, 2275, 2388
EcoRV GATATC 1 cut(s) 491
EcoT14I CCWWGG 3 cut(s) 14, 130, 770
EcoT22I ATGCAT 4 cut(s) 574, 720, 2009, 2118
EcoT38I GRGCYC 5 cut(s) 547, 590, 972, 2353, 2577
EgeI GGCGCC 1 cut(s) 30
EheI GGCGCC 1 cut(s) 30
ErhI CCWWGG 3 cut(s) 14, 130, 770
Esp3I CGTCTC 2 cut(s) 146, 294
FaqI GGGAC 6 cut(s) 69, 954, 1128, 1191, 2406, 2446
FauI CCCGC 3 cut(s) 95, 644, 2423
FbaI TGATCA 1 cut(s) 2400
FokI GGATG 9 cut(s) 131, 1031, 1397, 1480, 1914, 2198, 2381, 2487, 2578
FriOI GRGCYC 5 cut(s) 547, 590, 972, 2353, 2577
FspBI CTAG 6 cut(s) 261, 579, 612, 692, 1064, 1287
GlaI GCGC 5 cut(s) 30, 94, 181, 687, 1790
GsaI CCCAGC 3 cut(s) 213, 363, 2193
GsuI CTGGAG 3 cut(s) 702, 774, 909
HaeII RGCGCY 3 cut(s) 32, 183, 689
HapII CCGG 4 cut(s) 26, 1255, 1824, 2528
HgaI GACGC 4 cut(s) 83, 707, 965, 2350
HhaI GCGC 5 cut(s) 31, 95, 182, 688, 1791
Hin1I GRCGYC 2 cut(s) 29, 75
Hin6I GCGC 5 cut(s) 29, 93, 180, 686, 1789
HinP1I GCGC 5 cut(s) 29, 93, 180, 686, 1789
HincII GTYRAC 1 cut(s) 1314
HindII GTYRAC 1 cut(s) 1314
HindIII AAGCTT 1 cut(s) 2162
HinfI GANTC 6 cut(s) 525, 1204, 1519, 1661, 2047, 2052
HpaII CCGG 4 cut(s) 26, 1255, 1824, 2528
Hpy166II GTNNAC 5 cut(s) 741, 1196, 1314, 1560, 1942
Hpy188III TCNNGA 8 cut(s) 47, 49, 548, 681, 1931, 2354, 2540, 2627
Hpy8I GTNNAC 5 cut(s) 741, 1196, 1314, 1560, 1942
Hpy99I CGWCG 5 cut(s) 71, 74, 77, 116, 981
HpyAV CCTTC 7 cut(s) 167, 587, 615, 774, 845, 1088, 1424
HpyCH4III ACNGT 7 cut(s) 288, 502, 1135, 1479, 1723, 1773, 2397
HpyCH4IV ACGT 3 cut(s) 384, 1835, 1951
HpySE526I ACGT 3 cut(s) 384, 1835, 1951
Hsp92I GRCGYC 2 cut(s) 29, 75
HspAI GCGC 5 cut(s) 29, 93, 180, 686, 1789
KasI GGCGCC 1 cut(s) 28
Ksp22I TGATCA 1 cut(s) 2400
LmnI GCTCC 5 cut(s) 683, 967, 1714, 1911, 2572
LweI GCATC 6 cut(s) 207, 526, 1007, 1936, 2176, 2556
MaeI CTAG 6 cut(s) 261, 579, 612, 692, 1064, 1287
MaeII ACGT 3 cut(s) 384, 1835, 1951
MaeIII GTNAC 9 cut(s) 214, 496, 814, 916, 1391, 1454, 2284, 2305, 2523
MbiI CCGCTC 1 cut(s) 639
MboII GAAGA 7 cut(s) 142, 681, 1346, 1718, 1754, 1930, 2510
MflI RGATCY 3 cut(s) 1701, 2132, 2209
MhlI GDGCHC 7 cut(s) 326, 547, 590, 637, 972, 2353, 2577
Mly113I GGCGCC 1 cut(s) 29
MlyI GAGTC 3 cut(s) 534, 1213, 2041
MmeI TCCRAC 1 cut(s) 1362
Mph1103I ATGCAT 4 cut(s) 574, 720, 2009, 2118
MroXI GAANNNNTTC 1 cut(s) 1652
MseI TTAA 8 cut(s) 411, 1035, 1112, 1746, 1784, 1946, 2204, 2495
MslI CAYNNNNRTG 3 cut(s) 723, 2180, 2564
MspA1I CMGCKG 4 cut(s) 90, 863, 995, 1732
MspCI CTTAAG 1 cut(s) 1034
MspI CCGG 4 cut(s) 26, 1255, 1824, 2528
Mva1269I GAATGC 4 cut(s) 229, 364, 2009, 2363
MvaI CCWGG 9 cut(s) 81, 195, 250, 447, 541, 753, 1564, 2277, 2390
MvnI CGCG 1 cut(s) 308
NarI GGCGCC 1 cut(s) 29
NciI CCSGG 2 cut(s) 26, 1256
NcoI CCATGG 2 cut(s) 14, 130
NlaIV GGNNCC 6 cut(s) 30, 85, 136, 160, 743, 2487
NmuCI GTSAC 6 cut(s) 214, 916, 1391, 2284, 2305, 2523
NsiI ATGCAT 4 cut(s) 574, 720, 2009, 2118
NspI RCATGY 2 cut(s) 785, 815
NspV TTCGAA 1 cut(s) 8
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 1 cut(s) 2353
PaqCI CACCTGC 1 cut(s) 266
PasI CCCWGGG 1 cut(s) 2389
PceI AGGCCT 3 cut(s) 271, 604, 1618
PciI ACATGT 1 cut(s) 811
PcsI WCGNNNNNNNCGW 1 cut(s) 230
PctI GAATGC 4 cut(s) 229, 364, 2009, 2363
PdmI GAANNNNTTC 1 cut(s) 1652
PfeI GAWTC 3 cut(s) 1519, 1661, 2052
Pfl23II CGTACG 1 cut(s) 234
PflFI GACNNNGTC 1 cut(s) 441
PflMI CCANNNNNTGG 2 cut(s) 255, 469
PfoI TCCNGGA 1 cut(s) 1254
Ple19I CGATCG 1 cut(s) 1873
PleI GAGTC 3 cut(s) 533, 1212, 2041
PluTI GGCGCC 1 cut(s) 32
PpsI GAGTC 3 cut(s) 533, 1212, 2041
Ppu21I YACGTR 2 cut(s) 385, 1952
PpuMI RGGWCCY 2 cut(s) 353, 443
PscI ACATGT 1 cut(s) 811
PshAI GACNNNNGTC 1 cut(s) 1480
PshBI ATTAAT 1 cut(s) 2495
Psp124BI GAGCTC 3 cut(s) 972, 2353, 2577
Psp5II RGGWCCY 2 cut(s) 353, 443
Psp6I CCWGG 9 cut(s) 79, 193, 248, 445, 539, 751, 1562, 2275, 2388
PspEI GGTNACC 1 cut(s) 214
PspFI CCCAGC 3 cut(s) 209, 359, 2189
PspGI CCWGG 9 cut(s) 79, 193, 248, 445, 539, 751, 1562, 2275, 2388
PspLI CGTACG 1 cut(s) 234
PspN4I GGNNCC 6 cut(s) 30, 85, 136, 160, 743, 2487
PspPI GGNCC 9 cut(s) 83, 134, 353, 443, 449, 741, 1489, 2273, 2294
PspPPI RGGWCCY 2 cut(s) 353, 443
PstI CTGCAG 1 cut(s) 277
PsuI RGATCY 3 cut(s) 1701, 2132, 2209
PsyI GACNNNGTC 1 cut(s) 441
PvuI CGATCG 1 cut(s) 1873
PvuII CAGCTG 3 cut(s) 863, 995, 1732
RsaI GTAC 6 cut(s) 23, 43, 236, 432, 826, 1171
RsaNI GTAC 6 cut(s) 22, 42, 235, 431, 825, 1170
RseI CAYNNNNRTG 3 cut(s) 723, 2180, 2564
SacI GAGCTC 3 cut(s) 972, 2353, 2577
SaqAI TTAA 8 cut(s) 411, 1035, 1112, 1746, 1784, 1946, 2204, 2495
Sau96I GGNCC 9 cut(s) 83, 134, 353, 443, 449, 741, 1489, 2273, 2294
SbfI CCTGCAGG 1 cut(s) 277
ScaI AGTACT 1 cut(s) 1171
SchI GAGTC 3 cut(s) 534, 1213, 2041
SdaI CCTGCAGG 1 cut(s) 277
SduI GDGCHC 7 cut(s) 326, 547, 590, 637, 972, 2353, 2577
SfaNI GCATC 6 cut(s) 207, 526, 1007, 1936, 2176, 2556
SfcI CTRYAG 3 cut(s) 273, 1449, 2196
SfoI GGCGCC 1 cut(s) 30
Sfr274I CTCGAG 1 cut(s) 47
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 5 cut(s) 83, 353, 443, 741, 1489
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 3 cut(s) 723, 2180, 2564
SmlI CTYRAG 4 cut(s) 47, 1034, 1222, 2254
SmoI CTYRAG 4 cut(s) 47, 1034, 1222, 2254
Sse8387I CCTGCAGG 1 cut(s) 277
SseBI AGGCCT 3 cut(s) 271, 604, 1618
SspDI GGCGCC 1 cut(s) 28
SspMI CTAG 6 cut(s) 261, 579, 612, 692, 1064, 1287
SstI GAGCTC 3 cut(s) 972, 2353, 2577
StuI AGGCCT 3 cut(s) 271, 604, 1618
StyI CCWWGG 3 cut(s) 14, 130, 770
TaaI ACNGT 7 cut(s) 288, 502, 1135, 1479, 1723, 1773, 2397
TaiI ACGT 3 cut(s) 387, 1838, 1954
TaqI TCGA 8 cut(s) 8, 48, 1621, 1842, 1869, 1873, 2050, 2632
TatI WGTACW 2 cut(s) 430, 1169
TauI GCSGC 6 cut(s) 119, 180, 311, 314, 788, 987
TfiI GAWTC 3 cut(s) 1519, 1661, 2052
Tru1I TTAA 8 cut(s) 411, 1035, 1112, 1746, 1784, 1946, 2204, 2495
Tru9I TTAA 8 cut(s) 411, 1035, 1112, 1746, 1784, 1946, 2204, 2495
TscAI CASTG 6 cut(s) 511, 637, 737, 886, 1402, 1920
TseFI GTSAC 6 cut(s) 214, 916, 1391, 2284, 2305, 2523
Tsp45I GTSAC 6 cut(s) 214, 916, 1391, 2284, 2305, 2523
TspGWI ACGGA 1 cut(s) 2324
TspRI CASTG 6 cut(s) 511, 637, 737, 886, 1402, 1920
Tth111I GACNNNGTC 1 cut(s) 441
Van91I CCANNNNNTGG 2 cut(s) 255, 469
Vha464I CTTAAG 1 cut(s) 1034
VpaK11BI GGWCC 5 cut(s) 83, 353, 443, 741, 1489
VspI ATTAAT 1 cut(s) 2495
XapI RAATTY 2 cut(s) 1075, 2312
XceI RCATGY 2 cut(s) 785, 815
XhoI CTCGAG 1 cut(s) 47
XmnI GAANNNNTTC 1 cut(s) 1652
XspI CTAG 6 cut(s) 261, 579, 612, 692, 1064, 1287
ZrmI AGTACT 1 cut(s) 1171
Zsp2I ATGCAT 4 cut(s) 574, 720, 2009, 2118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.