RchiOBHm_Chr6g0292301

serine threonine-protein phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006473
Physical Location & Seq
Forward (+)
55229352 .. 55236551
7200 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26230

Sequence Viewer

Length: 2676 bp
ATGAGTGCGAAACCATGGCTGTACCCGGCTCCCACCTACCGTACTCTCGAGACCTATTGGGATACCGACGACGACGCGCCCGGCCCGCGATGCGGTCACACGCTCACCGCCGTCGCCGCCACCAAGACCCACGGCCCCCGCCTTATTCTATTCGGTGGCGCCACCGCCATTGAAGGCGGCGCCGCCTCCTCCGCCCCCGGCATCAGGCTAGCGGGTGTGACCAATTCTGTTCACTCCTACGATGTTCTCACCAGAAAGTGGACTAGAATCAGGCCGGCTGGTGAGCCACCGTCTCCGAGGGCGGCGCACGCTGCAGCTGCAGTGGGCACTATGGTGGTTTTTCAGGGTGGCATTGGTCCTGCTGGGCACTCCACGGATGATCTCTATGTGCTTGACATGGATAAGTTCAAGTGGCACAGAGTGGTTGTGCAAGGACAAGGACCTGGGCCTCGCTATGGCCATGTTATGGACTTAGTTGCTCAAAGATATCTTGTTACTGTCAGCGGCAATGATGGAAAAAGAGTTCTTTCTGATTCCTGGGCTCTAGATACTGCACAGAAACCTTACGTATGGGCTAAGCTGAGTCCAGAAGGGGATAGGCCTTCTGCTAGAATGTATGCAACAGCCAGTGCCCGCTCAGATGGCATGTTTTTGCTTTGTGGCGGAAGAGACGCTTCCGGGGCGCCTCTAGCTGATGCTTATGGACTGCTCATGCATAGAAATGGTCAGTGGGAGTGGACTCTCGCACCGGGAGTGTCTCCTTCACCAAGATATCAACATGCTGCGGTTTTTGTTGGTGCAAGATTACATGTAACGGGAGGTGTTCTCAGGGGAGGACGTGCTGTAGAAGGGGAAGGAGCTATTGCAGTATTGGACACTGCCGCTGGAGTTTGGTTAGATAGAAGCGGGCTTGTGACCTCCTCACGGACAGGCAAGGGACAAAATGACTATGATCCCCCTTTGGAGCTTATGCGTCGTTGTCGCCATGCAGCCGCATCTCTTGGTGTTCGTATTTACATTTATGGCGGTCTCAAGGGAGACATCCTGTTAGATGATTTTCTGGTTGCGGAAAATTCATCATTTCAATCTGAAATTAATTCTCCCGTATCAACTTCTGAGAAATCCCCATCTTTAACAAGTCCGAGAGCCTTTCATGCCAACACAAGTTCTTTTGGTGTATCACCATCTTCGGATAGTGAATCTGAGATACCCTCATCTAGTGGCTTGAGCATGGATAAAAATTCCATGGAGAAACTACGGCAGGCTTCTGCTGCCGAAGCTGAGGCAGCTAGTGCTGTTTGGCAAGCTGTGCAGTCCCAATCTTCCCCTCCTCCTGAAGAAACTTCTGTTTCAGATGACAACTCACAAATTGCAGAAACACTTTCGGATGGTAGTGACACTGAGGCAGATGTTCGCCTTCATCCCAGAGCTGTTGTTGTTGCCAAAGAGGCTGTAGGCAATTTGGGTGGCATGGTAAGACAGTTGTCTTTGGATCAATTCGAAAATGAGAGCAGACGAATGATTCCAATGAATAACGACTTATCATATCCTAACAAAAAGTTTACCAGGCAGAAGTCCCCACAGGGCTTACATAAGAAGATAATATCTACATTGCTGAGGCCAAGGAACTGGAAACCTCCGGTTCATAGGAAGTTTTTCCTCGATTCTTACGAAGTGGGAGAACTTTGTTATGCTGCTGAACAGATCTTTATGCAGGAGCAAACAGTTCTTCAGCTGAAAGCTCCTGTTAAAGTATTTGGCGATCTTCATGGACAGTTTGGTGATTTAATGCGCTTGTTTGATGAATATGGATTTCCATCTGCTGCAGGAGACATAACGTACATTGACTACTTGTTTTTGGGGGATTATGTTGATCGAGGACAGCACAGCTTGGAGACAATAACTCTACTCCTTGCACTCAAGGCGAGCTTCTGCTTTGAAATCGAGTATCCTGATAATGTTCACTTAATACGTGGAAACCATGAGGCTGCTGATATCAACGCACTCTTTGGTTTTCGTATTGAATGCATTGAGAGAATGGGAGAGAGTGATGGAATATGGGCATGGACAAGGTTCAATCAACTTTTCAACTACCTCCCACTGGCTGCACTAATTGAAAAGAAAATCATCTGTATGCATGGTGGCATTGGAAGATCTATAAATTCTGTAGAACAAATAGAGAAGCTTGAAAGACCCATTACAATGGATGCTGGGTCTATAATTTTAATGGATCTTCTATGGTCTGATCCTACAGAAAACGATGGTGTAGAGGGTCTCAGACCAAATGCAAGAGGGCCTGGTCTTGTCACTTTTGGGCCTGATCGTGTCACGGACTTCTGTAAGAGGAACAAATTACAGCTCATTATAAGAGCTCATGAATGTGTTATGGATGGTTTTGAACGGTTTGCCCAGGGACAATTGATCACTCTTTTTTCTGCCACCAACTATTGTGGAACGGCAAACAATGCTGGTGCAATACTGGTGGTTGGCAGGGGGCTGGTTGTAGTTCCAAAACTAATTCATCCGCTGCCACCTCCTCTTCAGTCACCGGAGACATCTCCTGAACGCGTCATAGATGACACATGGATGCAGGAGCTAAACATTCAGAGGCCGCCAACTCCTACTCGTGGTCGACCACAGCCTGATCTTGATCGGAACTCACTTGCATATATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Kelch_3 Kelch_4 Metallophos STPPase_N

Protein Analysis

891

Amino Acids

97.31

Weight (kDa)

5.97

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 21 - 218 4.8e-33 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 22 - 186 1.2e-25 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 25 - 223 1.9e-12 Attractin/LZTR1 beta-propeller
Kelch_HCF PF13854 72 - 346 1.1e-15 Host cell factor, Kelch-repeats domain
Beta-prop_FBX42 PF13415 173 - 355 3.2e-12 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 191 - 298 2.5e-10 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_TYW4 PF13418 201 - 277 7.9e-07 tRNA wybutosine-synthesizing protein 4, beta-propeller
Metallophos PF00149 582 - 795 8.1e-38 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2366
AccB1I GGYRCC 3 cut(s) 158, 179, 682
AccB7I CCANNNNNTGG 2 cut(s) 258, 466
AccBSI CCGCTC 1 cut(s) 636
AccI GTMKAC 1 cut(s) 2632
AccII CGCG 3 cut(s) 77, 88, 2568
AclWI GGATC 4 cut(s) 947, 1500, 2238, 2240
AcoI YGGCCR 1 cut(s) 457
AcsI RAATTY 3 cut(s) 1072, 1240, 2161
AcuI CTGAAG 3 cut(s) 1356, 1715, 2525
AcyI GRCGYC 3 cut(s) 159, 180, 683
AdeI CACNNNGTG 1 cut(s) 421
AfaI GTAC 3 cut(s) 23, 43, 1841
AfiI CCNNNNNNNGG 8 cut(s) 92, 204, 258, 455, 466, 924, 2101, 2627
AflIII ACRYGT 2 cut(s) 808, 2566
AjiI CACGTC 1 cut(s) 839
AjnI CCWGG 5 cut(s) 442, 536, 1565, 2296, 2409
AleI CACNNNNGTG 1 cut(s) 332
AloI GAACNNNNNNTCC 2 cut(s) 507, 539
Alw21I GWGCWC 1 cut(s) 2374
AlwI GGATC 4 cut(s) 947, 1500, 2238, 2240
Ama87I CYCGRG 1 cut(s) 47
ApoI RAATTY 3 cut(s) 1072, 1240, 2161
AseI ATTAAT 1 cut(s) 1095
Asp700I GAANNNNTTC 1 cut(s) 1655
AspLEI GCGC 6 cut(s) 79, 161, 182, 307, 685, 1794
AspS9I GGNCC 7 cut(s) 83, 134, 356, 440, 446, 2294, 2315
AsuC2I CCSGG 5 cut(s) 26, 81, 198, 679, 750
AsuHPI GGTGA 7 cut(s) 97, 241, 293, 756, 1173, 1793, 2538
AsuII TTCGAA 1 cut(s) 1500
AsuNHI GCTAGC 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 47
AvaII GGWCC 2 cut(s) 356, 440
BaeGI GKGCMC 3 cut(s) 329, 369, 634
BalI TGGCCA 1 cut(s) 459
BanI GGYRCC 3 cut(s) 158, 179, 682
BanII GRGCYC 2 cut(s) 544, 2374
BauI CACGAG 1 cut(s) 2625
Bbv12I GWGCWC 1 cut(s) 2374
BbvCI CCTCAGC 2 cut(s) 1281, 1616
BccI CCATC 9 cut(s) 506, 635, 1135, 1192, 1382, 1825, 2045, 2255, 2384
BceAI ACGGC 4 cut(s) 95, 148, 1274, 2472
BciT130I CCWGG 5 cut(s) 444, 538, 1567, 2298, 2411
BciVI GTATCC 2 cut(s) 55, 1959
BclI TGATCA 1 cut(s) 2421
BcnI CCSGG 5 cut(s) 26, 81, 198, 679, 750
BfaI CTAG 7 cut(s) 209, 264, 545, 609, 689, 1218, 1290
BfmI CTRYAG 7 cut(s) 312, 318, 843, 1452, 1824, 2166, 2250
BfoI RGCGCY 3 cut(s) 162, 183, 686
BfuI GTATCC 2 cut(s) 55, 1959
BglI GCCNNNNNGGC 1 cut(s) 1448
BglII AGATCT 2 cut(s) 1704, 2153
BlpI GCTNAGC 1 cut(s) 576
Bme18I GGWCC 2 cut(s) 356, 440
BmeT110I CYCGRG 1 cut(s) 47
BmgBI CACGTC 1 cut(s) 839
BmgT120I GGNCC 7 cut(s) 83, 134, 356, 440, 446, 2294, 2315
BmiI GGNNCC 5 cut(s) 30, 136, 160, 181, 684
BmsI GCATC 6 cut(s) 80, 210, 685, 1004, 2197, 2577
BmtI GCTAGC 1 cut(s) 212
BoxI GACNNNNGTC 1 cut(s) 1483
BplI GAGNNNNNCTC 4 cut(s) 810, 842, 1196, 1228
BpmI CTGGAG 1 cut(s) 906
Bpu10I CCTNAGC 2 cut(s) 1281, 1616
Bpu1102I GCTNAGC 1 cut(s) 576
Bpu14I TTCGAA 1 cut(s) 1500
BpuEI CTTGAG 3 cut(s) 1016, 1246, 1904
BpuMI CCSGG 5 cut(s) 26, 81, 198, 679, 750
BsaAI YACGTR 2 cut(s) 568, 1973
BsaBI GATNNNNATC 2 cut(s) 1816, 2649
BsaHI GRCGYC 3 cut(s) 159, 180, 683
BsaI GGTCTC 3 cut(s) 44, 1034, 2279
BsaWI WCCGGW 2 cut(s) 1639, 2548
Bsc4I CCNNNNNNNGG 8 cut(s) 92, 204, 258, 455, 466, 924, 2101, 2627
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 4 cut(s) 627, 1634, 2106, 2484
Bse3DI GCAATG 2 cut(s) 514, 1610
Bse8I GATNNNNATC 2 cut(s) 1816, 2649
BseBI CCWGG 5 cut(s) 444, 538, 1567, 2298, 2411
BseGI GGATG 8 cut(s) 382, 1041, 1393, 1420, 2212, 2395, 2521, 2592
BseJI GATNNNNATC 2 cut(s) 1816, 2649
BseLI CCNNNNNNNGG 8 cut(s) 92, 204, 258, 455, 466, 924, 2101, 2627
BseMI GCAATG 2 cut(s) 514, 1610
BseMII CTCAG 9 cut(s) 572, 651, 841, 1107, 1194, 1272, 1392, 1607, 2290
BseNI ACTGG 4 cut(s) 627, 1634, 2106, 2484
BseRI GAGGAG 4 cut(s) 178, 910, 1320, 2526
BseSI GKGCMC 3 cut(s) 329, 369, 634
BseYI CCCAGC 2 cut(s) 362, 2210
BsgI GTGCAG 3 cut(s) 537, 1331, 2091
Bsh1236I CGCG 3 cut(s) 77, 88, 2568
BshNI GGYRCC 3 cut(s) 158, 179, 682
BsiHKAI GWGCWC 1 cut(s) 2374
BsiHKCI CYCGRG 1 cut(s) 47
BsiSI CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1640, 2549
BslFI GGGAC 4 cut(s) 951, 1300, 1561, 2427
BslI CCNNNNNNNGG 8 cut(s) 92, 204, 258, 455, 466, 924, 2101, 2627
BsmBI CGTCTC 2 cut(s) 297, 663
BsmFI GGGAC 4 cut(s) 951, 1300, 1561, 2427
BsmI GAATGC 1 cut(s) 2030
Bso31I GGTCTC 3 cut(s) 44, 1034, 2279
BsoBI CYCGRG 1 cut(s) 47
Bsp119I TTCGAA 1 cut(s) 1500
Bsp1286I GDGCHC 5 cut(s) 329, 369, 544, 634, 2374
Bsp1720I GCTNAGC 1 cut(s) 576
Bsp19I CCATGG 2 cut(s) 14, 1245
BspCNI CTCAG 9 cut(s) 573, 650, 840, 1108, 1195, 1273, 1393, 1608, 2289
BspFNI CGCG 3 cut(s) 77, 88, 2568
BspHI TCATGA 1 cut(s) 2374
BspLI GGNNCC 5 cut(s) 30, 136, 160, 181, 684
BspMAI CTGCAG 3 cut(s) 316, 322, 1828
BspOI GCTAGC 1 cut(s) 212
BspPI GGATC 4 cut(s) 947, 1500, 2238, 2240
BspT104I TTCGAA 1 cut(s) 1500
BspT107I GGYRCC 3 cut(s) 158, 179, 682
BspTNI GGTCTC 3 cut(s) 44, 1034, 2279
BsrBI CCGCTC 1 cut(s) 636
BsrDI GCAATG 2 cut(s) 514, 1610
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 4 cut(s) 627, 1634, 2106, 2484
BssAI RCCGGY 1 cut(s) 274
BssNI GRCGYC 3 cut(s) 159, 180, 683
BssSI CACGAG 1 cut(s) 2625
BssT1I CCWWGG 3 cut(s) 14, 1245, 1622
Bst2BI CACGAG 1 cut(s) 2625
Bst2UI CCWGG 5 cut(s) 444, 538, 1567, 2298, 2411
Bst4CI ACNGT 7 cut(s) 41, 291, 499, 1482, 1726, 1776, 2403
Bst6I CTCTTC 2 cut(s) 661, 2544
BstACI GRCGYC 3 cut(s) 159, 180, 683
BstAPI GCANNNNNTGC 3 cut(s) 1292, 1309, 2465
BstBAI YACGTR 2 cut(s) 568, 1973
BstBI TTCGAA 1 cut(s) 1500
BstC8I GCNNGC 9 cut(s) 86, 210, 276, 309, 634, 908, 1263, 1305, 1927
BstDSI CCRYGG 4 cut(s) 14, 130, 372, 1245
BstF5I GGATG 8 cut(s) 382, 1041, 1393, 1420, 2212, 2395, 2521, 2592
BstFNI CGCG 3 cut(s) 77, 88, 2568
BstH2I RGCGCY 3 cut(s) 162, 183, 686
BstHHI GCGC 6 cut(s) 79, 161, 182, 307, 685, 1794
BstNI CCWGG 5 cut(s) 444, 538, 1567, 2298, 2411
BstNSI RCATGY 3 cut(s) 649, 782, 812
BstPAI GACNNNNGTC 1 cut(s) 1483
BstSFI CTRYAG 7 cut(s) 312, 318, 843, 1452, 1824, 2166, 2250
BstSLI GKGCMC 3 cut(s) 329, 369, 634
BstSNI TACGTA 1 cut(s) 568
BstUI CGCG 3 cut(s) 77, 88, 2568
BstX2I RGATCY 3 cut(s) 1704, 2153, 2230
BstXI CCANNNNNNTGG 2 cut(s) 1629, 2203
BstYI RGATCY 3 cut(s) 1704, 2153, 2230
BsuI GTATCC 2 cut(s) 55, 1959
BtgI CCRYGG 4 cut(s) 14, 130, 372, 1245
BtgZI GCGATG 1 cut(s) 103
BtrI CACGTC 1 cut(s) 839
BtsCI GGATG 8 cut(s) 382, 1041, 1393, 1420, 2212, 2395, 2521, 2592
BtsI GCAGTG 2 cut(s) 327, 876
BtsIMutI CAGTG 6 cut(s) 327, 634, 734, 876, 1398, 2099
Cac8I GCNNGC 9 cut(s) 86, 210, 276, 309, 634, 908, 1263, 1305, 1927
CciI TCATGA 1 cut(s) 2374
CfoI GCGC 6 cut(s) 79, 161, 182, 307, 685, 1794
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 7 cut(s) 83, 134, 356, 440, 446, 2294, 2315
CseI GACGC 4 cut(s) 83, 680, 962, 2557
Csp6I GTAC 3 cut(s) 22, 42, 1840
CspCI CAANNNNNGTGG 8 cut(s) 151, 186, 1447, 1482, 2431, 2463, 2466, 2498
CviQI GTAC 3 cut(s) 22, 42, 1840
DinI GGCGCC 3 cut(s) 160, 181, 684
DraIII CACNNNGTG 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 457
Eam1104I CTCTTC 2 cut(s) 661, 2544
EarI CTCTTC 2 cut(s) 661, 2544
EciI GGCGGA 2 cut(s) 181, 678
Ecl136II GAGCTC 1 cut(s) 2372
Eco105I TACGTA 1 cut(s) 568
Eco130I CCWWGG 3 cut(s) 14, 1245, 1622
Eco147I AGGCCT 1 cut(s) 601
Eco24I GRGCYC 2 cut(s) 544, 2374
Eco31I GGTCTC 3 cut(s) 44, 1034, 2279
Eco32I GATATC 3 cut(s) 488, 773, 1996
Eco47I GGWCC 2 cut(s) 356, 440
Eco53kI GAGCTC 1 cut(s) 2372
Eco57I CTGAAG 3 cut(s) 1356, 1715, 2525
Eco88I CYCGRG 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 2372
EcoO109I RGGNCCY 2 cut(s) 440, 2294
EcoRII CCWGG 5 cut(s) 442, 536, 1565, 2296, 2409
EcoRV GATATC 3 cut(s) 488, 773, 1996
EcoT14I CCWWGG 3 cut(s) 14, 1245, 1622
EcoT22I ATGCAT 3 cut(s) 717, 2030, 2139
EcoT38I GRGCYC 2 cut(s) 544, 2374
EgeI GGCGCC 3 cut(s) 160, 181, 684
EheI GGCGCC 3 cut(s) 160, 181, 684
ErhI CCWWGG 3 cut(s) 14, 1245, 1622
Esp3I CGTCTC 2 cut(s) 297, 663
FalI AAGNNNNNCTT 4 cut(s) 658, 690, 1913, 1945
FaqI GGGAC 4 cut(s) 951, 1300, 1561, 2427
FauI CCCGC 5 cut(s) 93, 146, 205, 641, 899
FbaI TGATCA 1 cut(s) 2421
FblI GTMKAC 1 cut(s) 2632
FokI GGATG 8 cut(s) 389, 1028, 1400, 1407, 2219, 2402, 2508, 2599
FriOI GRGCYC 2 cut(s) 544, 2374
FspBI CTAG 7 cut(s) 209, 264, 545, 609, 689, 1218, 1290
GlaI GCGC 6 cut(s) 78, 160, 181, 306, 684, 1793
GsaI CCCAGC 2 cut(s) 366, 2214
GsuI CTGGAG 1 cut(s) 906
HaeII RGCGCY 3 cut(s) 162, 183, 686
HapII CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1640, 2549
HgaI GACGC 4 cut(s) 83, 680, 962, 2557
HhaI GCGC 6 cut(s) 79, 161, 182, 307, 685, 1794
Hin1I GRCGYC 3 cut(s) 159, 180, 683
Hin6I GCGC 6 cut(s) 77, 159, 180, 305, 683, 1792
HinP1I GCGC 6 cut(s) 77, 159, 180, 305, 683, 1792
HincII GTYRAC 1 cut(s) 2633
HindII GTYRAC 1 cut(s) 2633
HindIII AAGCTT 1 cut(s) 2183
HinfI GANTC 7 cut(s) 267, 533, 583, 739, 1199, 1522, 1664
HpaII CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1640, 2549
HphI GGTGA 7 cut(s) 97, 241, 293, 756, 1173, 1793, 2538
Hpy166II GTNNAC 6 cut(s) 232, 261, 738, 1563, 1963, 2633
Hpy188III TCNNGA 9 cut(s) 47, 49, 545, 587, 1334, 1952, 2375, 2561, 2648
Hpy8I GTNNAC 6 cut(s) 232, 261, 738, 1563, 1963, 2633
Hpy99I CGWCG 5 cut(s) 71, 74, 77, 116, 978
HpyAV CCTTC 7 cut(s) 167, 584, 612, 771, 842, 848, 1427
HpyCH4III ACNGT 7 cut(s) 41, 291, 499, 1482, 1726, 1776, 2403
HpyCH4IV ACGT 4 cut(s) 567, 838, 1838, 1972
HpySE526I ACGT 4 cut(s) 567, 838, 1838, 1972
Hsp92I GRCGYC 3 cut(s) 159, 180, 683
HspAI GCGC 6 cut(s) 77, 159, 180, 305, 683, 1792
KasI GGCGCC 3 cut(s) 158, 179, 682
KroI GCCGGC 1 cut(s) 274
KroNI GCCGGC 1 cut(s) 276
Ksp22I TGATCA 1 cut(s) 2421
LmnI GCTCC 6 cut(s) 34, 857, 964, 1717, 1747, 2593
LweI GCATC 6 cut(s) 80, 210, 685, 1004, 2197, 2577
MaeI CTAG 7 cut(s) 209, 264, 545, 609, 689, 1218, 1290
MaeII ACGT 4 cut(s) 567, 838, 1838, 1972
MaeIII GTNAC 9 cut(s) 95, 217, 493, 811, 913, 1394, 2305, 2326, 2544
MbiI CCGCTC 1 cut(s) 636
MfeI CAATTG 1 cut(s) 2417
MflI RGATCY 3 cut(s) 1704, 2153, 2230
MhlI GDGCHC 5 cut(s) 329, 369, 544, 634, 2374
MlsI TGGCCA 1 cut(s) 459
MluI ACGCGT 1 cut(s) 2566
MluNI TGGCCA 1 cut(s) 459
Mly113I GGCGCC 3 cut(s) 159, 180, 683
MlyI GAGTC 2 cut(s) 592, 733
Mox20I TGGCCA 1 cut(s) 459
Mph1103I ATGCAT 3 cut(s) 717, 2030, 2139
MroNI GCCGGC 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 1655
MscI TGGCCA 1 cut(s) 459
MseI TTAA 6 cut(s) 1095, 1133, 1749, 1787, 1967, 2225
MslI CAYNNNNRTG 6 cut(s) 332, 720, 2132, 2201, 2379, 2585
Msp20I TGGCCA 1 cut(s) 459
MspA1I CMGCKG 5 cut(s) 317, 504, 884, 1735, 2527
MspI CCGG 8 cut(s) 26, 81, 198, 275, 678, 749, 1640, 2549
MunI CAATTG 1 cut(s) 2417
Mva1269I GAATGC 1 cut(s) 2030
MvaI CCWGG 5 cut(s) 444, 538, 1567, 2298, 2411
MvnI CGCG 3 cut(s) 77, 88, 2568
NaeI GCCGGC 1 cut(s) 276
NarI GGCGCC 3 cut(s) 159, 180, 683
NciI CCSGG 5 cut(s) 26, 81, 198, 679, 750
NcoI CCATGG 2 cut(s) 14, 1245
NgoMIV GCCGGC 1 cut(s) 274
NheI GCTAGC 1 cut(s) 208
NlaIV GGNNCC 5 cut(s) 30, 136, 160, 181, 684
NmuCI GTSAC 7 cut(s) 95, 217, 913, 1394, 2305, 2326, 2544
NsiI ATGCAT 3 cut(s) 717, 2030, 2139
NspI RCATGY 3 cut(s) 649, 782, 812
NspV TTCGAA 1 cut(s) 1500
OliI CACNNNNGTG 1 cut(s) 332
PaeR7I CTCGAG 1 cut(s) 47
PagI TCATGA 1 cut(s) 2374
PasI CCCWGGG 1 cut(s) 2410
PceI AGGCCT 1 cut(s) 601
PciI ACATGT 1 cut(s) 808
PcsI WCGNNNNNNNCGW 1 cut(s) 1668
PctI GAATGC 1 cut(s) 2030
PdiI GCCGGC 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 1655
PfeI GAWTC 5 cut(s) 267, 533, 1199, 1522, 1664
PflMI CCANNNNNTGG 2 cut(s) 258, 466
PleI GAGTC 2 cut(s) 591, 733
PluTI GGCGCC 3 cut(s) 162, 183, 686
PpsI GAGTC 2 cut(s) 591, 733
Ppu21I YACGTR 2 cut(s) 568, 1973
PpuMI RGGWCCY 1 cut(s) 440
PscI ACATGT 1 cut(s) 808
PshAI GACNNNNGTC 1 cut(s) 1483
PshBI ATTAAT 1 cut(s) 1095
PsiI TTATAA 1 cut(s) 2366
Psp124BI GAGCTC 1 cut(s) 2374
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 5 cut(s) 442, 536, 1565, 2296, 2409
PspFI CCCAGC 2 cut(s) 362, 2210
PspGI CCWGG 5 cut(s) 442, 536, 1565, 2296, 2409
PspN4I GGNNCC 5 cut(s) 30, 136, 160, 181, 684
PspPI GGNCC 7 cut(s) 83, 134, 356, 440, 446, 2294, 2315
PspPPI RGGWCCY 1 cut(s) 440
PstI CTGCAG 3 cut(s) 316, 322, 1828
PsuI RGATCY 3 cut(s) 1704, 2153, 2230
PvuII CAGCTG 2 cut(s) 317, 1735
RsaI GTAC 3 cut(s) 23, 43, 1841
RsaNI GTAC 3 cut(s) 22, 42, 1840
RseI CAYNNNNRTG 6 cut(s) 332, 720, 2132, 2201, 2379, 2585
SacI GAGCTC 1 cut(s) 2374
SalI GTCGAC 1 cut(s) 2631
SaqAI TTAA 6 cut(s) 1095, 1133, 1749, 1787, 1967, 2225
Sau96I GGNCC 7 cut(s) 83, 134, 356, 440, 446, 2294, 2315
SchI GAGTC 2 cut(s) 592, 733
SduI GDGCHC 5 cut(s) 329, 369, 544, 634, 2374
SfaNI GCATC 6 cut(s) 80, 210, 685, 1004, 2197, 2577
SfcI CTRYAG 7 cut(s) 312, 318, 843, 1452, 1824, 2166, 2250
SfoI GGCGCC 3 cut(s) 160, 181, 684
Sfr274I CTCGAG 1 cut(s) 47
SfuI TTCGAA 1 cut(s) 1500
SinI GGWCC 2 cut(s) 356, 440
SlaI CTCGAG 1 cut(s) 47
SmiMI CAYNNNNRTG 6 cut(s) 332, 720, 2132, 2201, 2379, 2585
SmlI CTYRAG 4 cut(s) 47, 1031, 1225, 1919
SmoI CTYRAG 4 cut(s) 47, 1031, 1225, 1919
SnaBI TACGTA 1 cut(s) 568
SseBI AGGCCT 1 cut(s) 601
SspDI GGCGCC 3 cut(s) 158, 179, 682
SspMI CTAG 7 cut(s) 209, 264, 545, 609, 689, 1218, 1290
SstI GAGCTC 1 cut(s) 2374
StuI AGGCCT 1 cut(s) 601
StyI CCWWGG 3 cut(s) 14, 1245, 1622
TaaI ACNGT 7 cut(s) 41, 291, 499, 1482, 1726, 1776, 2403
TaiI ACGT 4 cut(s) 570, 841, 1841, 1975
TaqI TCGA 6 cut(s) 48, 1500, 1662, 1876, 1944, 2632
TauI GCSGC 8 cut(s) 119, 180, 185, 305, 507, 884, 995, 2614
TfiI GAWTC 5 cut(s) 267, 533, 1199, 1522, 1664
Tru1I TTAA 6 cut(s) 1095, 1133, 1749, 1787, 1967, 2225
Tru9I TTAA 6 cut(s) 1095, 1133, 1749, 1787, 1967, 2225
TscAI CASTG 6 cut(s) 327, 634, 734, 883, 1405, 2106
TseFI GTSAC 7 cut(s) 95, 217, 913, 1394, 2305, 2326, 2544
Tsp45I GTSAC 7 cut(s) 95, 217, 913, 1394, 2305, 2326, 2544
TspDTI ATGAA 9 cut(s) 1065, 1142, 1409, 1544, 1634, 1757, 1818, 2391, 2510
TspGWI ACGGA 3 cut(s) 389, 940, 2345
TspRI CASTG 6 cut(s) 327, 634, 734, 883, 1405, 2106
Van91I CCANNNNNTGG 2 cut(s) 258, 466
VpaK11BI GGWCC 2 cut(s) 356, 440
VspI ATTAAT 1 cut(s) 1095
XapI RAATTY 3 cut(s) 1072, 1240, 2161
XbaI TCTAGA 1 cut(s) 544
XceI RCATGY 3 cut(s) 649, 782, 812
XhoI CTCGAG 1 cut(s) 47
XmiI GTMKAC 1 cut(s) 2632
XmnI GAANNNNTTC 1 cut(s) 1655
XspI CTAG 7 cut(s) 209, 264, 545, 609, 689, 1218, 1290
Zsp2I ATGCAT 3 cut(s) 717, 2030, 2139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.