FvH4_2g25370
NAC Family

Dol-P-Man Man(5)GlcNAc(2)-PP-Dol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006111
Physical Location & Seq
Reverse (-)
20612684 .. 20616060
3377 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g25370.t1

Sequence Viewer

Length: 1299 bp
ATGGCGGTCAAATCGGCGCGGCGGGCGAAATCCTCGCAGCACCGCCAAATTTCCAAGGCCTGGATCCCCAAACTGTTCCGAAACCCCAAGGTCGCCTCTGCCTCCGCCTTACTCGTCTTCGATGCGGTCCTCACCGCCCTTATCATCGCCTACGTCCCCTATACGAAGATCGATTGGGACGCGTACATGTCTCAGGTTAGTGGATTTATTGGTGGAGAAAGAGACTATAGTAATTTGAAAGGAGACACAGGGCCACTGGTGTATCCAGCTGGTTTTTTGTATGCTTATTCTGCTATTCAGTATGTTACTGGAAGCCAAGTTCATCCAGCTCAGATTTTGTTCGGGGTTTTGTACATCGTAAACTTGGGAATCATGTTATACATCTACTTGAAGACTGATGTGGTTCCATGGTGGGCACTTGCCTTGCTTTGTCTATCAAAAAGAATGCATTCTATTTTTGTGCTCCGGCTTTTTAATGACTGTTTGGCCATGTTGCTCCTGCATGCTTCATTAGCCTCGTTTCTTCACCAAAGGTGGCATCTAGGATTGATCATTTTCAGTGGTGCTGTTTCAATAAAGATGAATGTGCTCCTCTATGCTCCACCTCTGCTGCTCCTCATGCTGAAGGCTATGGATATCAGTGGAGTTATATCAGCTTTGTTTGGTGCAGCTCTTGTCCAGATACTGTTGGGGTTGCCTTTCATCATGTCTCATCCGGTTGCATACATTTCAAGAGCCTTTAATCTTGGCCGTGTATTCATTCACTTCTGGTCTGTTAATTTCAAGTTCGTTCCTGAGCCAATATTTGTCTCCAAGGCATTTGCAATCTCTTTGCTGGTTGTACACCTTGTTCTACTTGCAGCTTTTGCTCACTATAGATGGTGCAAGCATGAAGGAGGAGTTTTTAAATTTTTGTATTCTAGACTTGTTTCTATAAAACTCAAATTTGCTCTTACAAATACCCGTTCCTACAAGGCTCTCAAGAAAGAATATATTGTGACAACTATGTTCGTTGGAAATTTCATCGGCATTTTATGTGCCCGATCACTGCATTATCAGTTCTACTCTTGGTATTTCCACACGTTACCTTATCTACTGTGGAAAACGCCTTTTCCTTCCTTTCTACGGGTAATATTGTTTACCGGAGTGGAGCTGTGCTGGAATGTGTATCCTTCCAACCTTTACTCGTCAGCACTGCTTCTTGGTCTGCACTTAGTAATATTATTTGGTCTGTGGTCTGCCCCATCCGAATATCCTTATGTTGACAAGAAAGTATCAACTCAAGACAAACACAAATGA

Protein Analysis

433

Amino Acids

49.02

Weight (kDa)

9.77

Isoelectric Point (pI)

36.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALG3 PF05208 36 - 397 1.7e-137 ALG3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 60
AccII CGCG 2 cut(s) 19, 182
AciI CCGC 7 cut(s) 5, 19, 22, 43, 105, 125, 135
AclWI GGATC 2 cut(s) 58, 71
AcoI YGGCCR 2 cut(s) 486, 748
AcsI RAATTY 4 cut(s) 48, 908, 944, 1018
AcuI CTGAAG 1 cut(s) 644
AfaI GTAC 3 cut(s) 185, 353, 843
AfiI CCNNNNNNNGG 2 cut(s) 60, 1125
AflIII ACRYGT 3 cut(s) 180, 186, 1080
AgsI TTSAA 5 cut(s) 238, 391, 573, 732, 784
AjnI CCWGG 1 cut(s) 59
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
AluBI AGCT 6 cut(s) 269, 329, 656, 671, 863, 1153
AluI AGCT 6 cut(s) 269, 329, 656, 671, 863, 1153
Alw21I GWGCWC 2 cut(s) 465, 591
Alw26I GTCTC 5 cut(s) 195, 216, 237, 714, 814
AlwI GGATC 2 cut(s) 58, 71
AlwNI CAGNNNCTG 1 cut(s) 685
AoxI GGCC 4 cut(s) 57, 251, 486, 748
ApeKI GCWGC 4 cut(s) 37, 610, 668, 860
ApoI RAATTY 4 cut(s) 48, 908, 944, 1018
Asp700I GAANNNNTTC 1 cut(s) 448
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 2 cut(s) 127, 251
AsuHPI GGTGA 2 cut(s) 124, 518
AvaII GGWCC 1 cut(s) 127
BaeGI GKGCMC 2 cut(s) 418, 1042
BaeI ACNNNNGTAYC 2 cut(s) 245, 278
BalI TGGCCA 1 cut(s) 488
BamHI GGATCC 1 cut(s) 63
BbsI GAAGAC 2 cut(s) 109, 398
Bbv12I GWGCWC 2 cut(s) 465, 591
BbvI GCAGC 4 cut(s) 49, 597, 680, 872
BccI CCATC 2 cut(s) 873, 1252
BceAI ACGGC 1 cut(s) 735
BciT130I CCWGG 1 cut(s) 61
BciVI GTATCC 2 cut(s) 273, 1179
BclI TGATCA 1 cut(s) 549
BcoDI GTCTC 5 cut(s) 195, 216, 237, 714, 814
BfaI CTAG 2 cut(s) 542, 921
BfmI CTRYAG 2 cut(s) 226, 874
BfuI GTATCC 2 cut(s) 273, 1179
BisI GCNGC 5 cut(s) 20, 38, 611, 669, 861
BlsI GCNGC 5 cut(s) 21, 39, 612, 670, 862
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 127, 251
BmiI GGNNCC 2 cut(s) 65, 405
BmrFI CCNGG 1 cut(s) 61
BmsI GCATC 2 cut(s) 112, 547
BpiI GAAGAC 2 cut(s) 109, 398
Bpu10I CCTNAGC 1 cut(s) 795
BpuEI CTTGAG 2 cut(s) 965, 1266
Bsa29I ATCGAT 1 cut(s) 171
BsaJI CCNNGG 4 cut(s) 54, 87, 407, 813
BsaWI WCCGGW 2 cut(s) 715, 1142
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 1125
Bse1I ACTGG 2 cut(s) 261, 313
BseBI CCWGG 1 cut(s) 61
BseCI ATCGAT 1 cut(s) 171
BseDI CCNNGG 4 cut(s) 54, 87, 407, 813
BseGI GGATG 3 cut(s) 322, 712, 1244
BseLI CCNNNNNNNGG 2 cut(s) 60, 1125
BseMII CTCAG 3 cut(s) 206, 344, 786
BseNI ACTGG 2 cut(s) 261, 313
BseRI GAGGAG 3 cut(s) 581, 605, 912
BseSI GKGCMC 2 cut(s) 418, 1042
BseXI GCAGC 4 cut(s) 49, 597, 680, 872
BsgI GTGCAG 2 cut(s) 687, 1193
Bsh1236I CGCG 2 cut(s) 19, 182
BshFI GGCC 4 cut(s) 59, 253, 488, 750
BshVI ATCGAT 1 cut(s) 171
BsiHKAI GWGCWC 2 cut(s) 465, 591
BsiSI CCGG 3 cut(s) 466, 716, 1143
BslFI GGGAC 2 cut(s) 140, 191
BslI CCNNNNNNNGG 2 cut(s) 60, 1125
BsmAI GTCTC 5 cut(s) 195, 216, 237, 714, 814
BsmFI GGGAC 2 cut(s) 140, 191
BsmI GAATGC 2 cut(s) 448, 450
BsnI GGCC 4 cut(s) 59, 253, 488, 750
Bsp1286I GDGCHC 4 cut(s) 418, 465, 591, 1042
Bsp1407I TGTACA 2 cut(s) 351, 841
Bsp143I GATC 4 cut(s) 63, 168, 549, 1043
Bsp19I CCATGG 1 cut(s) 407
BspACI CCGC 7 cut(s) 5, 19, 22, 43, 105, 125, 135
BspANI GGCC 4 cut(s) 59, 253, 488, 750
BspCNI CTCAG 3 cut(s) 205, 343, 787
BspDI ATCGAT 1 cut(s) 171
BspFNI CGCG 2 cut(s) 19, 182
BspLI GGNNCC 2 cut(s) 65, 405
BspPI GGATC 2 cut(s) 58, 71
BsrGI TGTACA 2 cut(s) 351, 841
BsrI ACTGG 2 cut(s) 261, 313
BssECI CCNNGG 4 cut(s) 54, 87, 407, 813
BssMI GATC 4 cut(s) 63, 168, 549, 1043
BssT1I CCWWGG 4 cut(s) 54, 87, 407, 813
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 4 cut(s) 75, 482, 687, 1098
BstAPI GCANNNNNTGC 1 cut(s) 866
BstAUI TGTACA 2 cut(s) 351, 841
BstC8I GCNNGC 3 cut(s) 24, 504, 887
BstDEI CTNAG 4 cut(s) 192, 330, 795, 1213
BstDSI CCRYGG 1 cut(s) 407
BstF5I GGATG 3 cut(s) 322, 712, 1244
BstFNI CGCG 2 cut(s) 19, 182
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 4 cut(s) 66, 171, 552, 1046
BstMAI GTCTC 5 cut(s) 195, 216, 237, 714, 814
BstMBI GATC 4 cut(s) 63, 168, 549, 1043
BstMWI GCNNNNNNNGC 5 cut(s) 23, 290, 512, 619, 866
BstNI CCWGG 1 cut(s) 61
BstNSI RCATGY 2 cut(s) 190, 506
BstSCI CCNGG 1 cut(s) 59
BstSFI CTRYAG 2 cut(s) 226, 874
BstSLI GKGCMC 2 cut(s) 418, 1042
BstUI CGCG 2 cut(s) 19, 182
BstV1I GCAGC 4 cut(s) 49, 597, 680, 872
BstV2I GAAGAC 2 cut(s) 109, 398
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
Bsu15I ATCGAT 1 cut(s) 171
BsuI GTATCC 2 cut(s) 273, 1179
BsuRI GGCC 4 cut(s) 59, 253, 488, 750
BsuTUI ATCGAT 1 cut(s) 171
BtgI CCRYGG 1 cut(s) 407
BtgZI GCGATG 1 cut(s) 130
BtsCI GGATG 3 cut(s) 322, 712, 1244
BtsI GCAGTG 2 cut(s) 1046, 1193
BtsIMutI CAGTG 5 cut(s) 254, 565, 646, 1046, 1193
Cac8I GCNNGC 3 cut(s) 24, 504, 887
CaiI CAGNNNCTG 1 cut(s) 685
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 2 cut(s) 127, 251
ClaI ATCGAT 1 cut(s) 171
CseI GACGC 1 cut(s) 188
Csp6I GTAC 3 cut(s) 184, 352, 842
CviAII CATG 8 cut(s) 187, 373, 408, 490, 503, 619, 706, 890
CviQI GTAC 3 cut(s) 184, 352, 842
DdeI CTNAG 4 cut(s) 192, 330, 795, 1213
DpnI GATC 4 cut(s) 65, 170, 551, 1045
DpnII GATC 4 cut(s) 63, 168, 549, 1043
DraI TTTAAA 1 cut(s) 907
EaeI YGGCCR 2 cut(s) 486, 748
EciI GGCGGA 1 cut(s) 94
Eco130I CCWWGG 4 cut(s) 54, 87, 407, 813
Eco147I AGGCCT 1 cut(s) 59
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 644
EcoRII CCWGG 1 cut(s) 59
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 4 cut(s) 54, 87, 407, 813
EcoT22I ATGCAT 1 cut(s) 450
ErhI CCWWGG 4 cut(s) 54, 87, 407, 813
FaeI CATG 8 cut(s) 190, 376, 411, 493, 506, 622, 709, 893
FaqI GGGAC 2 cut(s) 140, 191
FatI CATG 8 cut(s) 186, 372, 407, 489, 502, 618, 705, 889
FauI CCCGC 1 cut(s) 15
FbaI TGATCA 1 cut(s) 549
Fnu4HI GCNGC 5 cut(s) 20, 38, 611, 669, 861
FokI GGATG 3 cut(s) 309, 699, 1231
Fsp4HI GCNGC 5 cut(s) 20, 38, 611, 669, 861
FspBI CTAG 2 cut(s) 542, 921
GlaI GCGC 1 cut(s) 18
GluI GCNGC 5 cut(s) 20, 38, 611, 669, 861
HaeIII GGCC 4 cut(s) 59, 253, 488, 750
HapII CCGG 3 cut(s) 466, 716, 1143
HgaI GACGC 1 cut(s) 188
HhaI GCGC 1 cut(s) 19
Hin1II CATG 8 cut(s) 190, 376, 411, 493, 506, 622, 709, 893
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HincII GTYRAC 1 cut(s) 1264
HindII GTYRAC 1 cut(s) 1264
HinfI GANTC 1 cut(s) 369
HpaII CCGG 3 cut(s) 466, 716, 1143
HphI GGTGA 2 cut(s) 124, 518
Hpy166II GTNNAC 4 cut(s) 361, 844, 1140, 1264
Hpy188I TCNGA 3 cut(s) 80, 333, 1249
Hpy188III TCNNGA 6 cut(s) 679, 732, 794, 921, 982, 1283
Hpy8I GTNNAC 4 cut(s) 361, 844, 1140, 1264
HpyAV CCTTC 4 cut(s) 619, 887, 1125, 1182
HpyCH4III ACNGT 4 cut(s) 75, 482, 687, 1098
HpyCH4IV ACGT 2 cut(s) 153, 1082
HpyCH4V TGCA 9 cut(s) 448, 502, 668, 722, 824, 860, 885, 1051, 1210
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 290, 512, 619, 866
HpyF3I CTNAG 4 cut(s) 192, 330, 795, 1213
HpySE526I ACGT 2 cut(s) 153, 1082
Hsp92II CATG 8 cut(s) 190, 376, 411, 493, 506, 622, 709, 893
HspAI GCGC 1 cut(s) 17
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 4 cut(s) 63, 168, 549, 1043
LmnI GCTCC 6 cut(s) 468, 501, 594, 604, 618, 1150
Lsp1109I GCAGC 4 cut(s) 49, 597, 680, 872
LweI GCATC 2 cut(s) 112, 547
MaeI CTAG 2 cut(s) 542, 921
MaeII ACGT 2 cut(s) 153, 1082
MaeIII GTNAC 3 cut(s) 304, 997, 1083
MalI GATC 4 cut(s) 65, 170, 551, 1045
MboI GATC 4 cut(s) 63, 168, 549, 1043
MboII GAAGA 4 cut(s) 109, 178, 403, 515
MflI RGATCY 1 cut(s) 63
MhlI GDGCHC 4 cut(s) 418, 465, 591, 1042
MlsI TGGCCA 1 cut(s) 488
MluCI AATT 6 cut(s) 48, 232, 778, 908, 944, 1018
MluI ACGCGT 1 cut(s) 180
MluNI TGGCCA 1 cut(s) 488
MmeI TCCRAC 2 cut(s) 994, 1200
MnlI CCTC 9 cut(s) 43, 106, 112, 140, 526, 602, 615, 626, 890
Mox20I TGGCCA 1 cut(s) 488
Mph1103I ATGCAT 1 cut(s) 450
MroXI GAANNNNTTC 1 cut(s) 448
MscI TGGCCA 1 cut(s) 488
MseI TTAA 4 cut(s) 474, 741, 777, 906
Msp20I TGGCCA 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 269
MspI CCGG 3 cut(s) 466, 716, 1143
MspR9I CCNGG 1 cut(s) 61
Mva1269I GAATGC 2 cut(s) 448, 450
MvaI CCWGG 1 cut(s) 61
MvnI CGCG 2 cut(s) 19, 182
MwoI GCNNNNNNNGC 5 cut(s) 23, 290, 512, 619, 866
NcoI CCATGG 1 cut(s) 407
NdeII GATC 4 cut(s) 63, 168, 549, 1043
NlaIII CATG 8 cut(s) 190, 376, 411, 493, 506, 622, 709, 893
NlaIV GGNNCC 2 cut(s) 65, 405
NmuCI GTSAC 1 cut(s) 997
NsiI ATGCAT 1 cut(s) 450
NspI RCATGY 2 cut(s) 190, 506
PaeI GCATGC 1 cut(s) 506
PceI AGGCCT 1 cut(s) 59
PciI ACATGT 1 cut(s) 186
PctI GAATGC 2 cut(s) 448, 450
PdmI GAANNNNTTC 1 cut(s) 448
PfeI GAWTC 1 cut(s) 369
PflMI CCANNNNNTGG 1 cut(s) 60
PkrI GCNGC 5 cut(s) 21, 39, 612, 670, 862
PscI ACATGT 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 65, 405
PspPI GGNCC 2 cut(s) 127, 251
PsrI GAACNNNNNNTAC 2 cut(s) 834, 866
PstNI CAGNNNCTG 1 cut(s) 685
PsuI RGATCY 1 cut(s) 63
PvuII CAGCTG 1 cut(s) 269
RsaI GTAC 3 cut(s) 185, 353, 843
RsaNI GTAC 3 cut(s) 184, 352, 842
SaqAI TTAA 4 cut(s) 474, 741, 777, 906
SatI GCNGC 5 cut(s) 20, 38, 611, 669, 861
Sau3AI GATC 4 cut(s) 63, 168, 549, 1043
Sau96I GGNCC 2 cut(s) 127, 251
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 4 cut(s) 418, 465, 591, 1042
SfaNI GCATC 2 cut(s) 112, 547
SfcI CTRYAG 2 cut(s) 226, 874
SinI GGWCC 1 cut(s) 127
SmlI CTYRAG 2 cut(s) 980, 1281
SmoI CTYRAG 2 cut(s) 980, 1281
SphI GCATGC 1 cut(s) 506
Sse9I AATT 6 cut(s) 48, 232, 778, 908, 944, 1018
SseBI AGGCCT 1 cut(s) 59
SsiI CCGC 7 cut(s) 5, 19, 22, 43, 105, 125, 135
SspI AATATT 3 cut(s) 804, 1134, 1221
SspMI CTAG 2 cut(s) 542, 921
StuI AGGCCT 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 4 cut(s) 54, 87, 407, 813
TaaI ACNGT 4 cut(s) 75, 482, 687, 1098
TaiI ACGT 2 cut(s) 156, 1085
TaqI TCGA 2 cut(s) 120, 171
TasI AATT 6 cut(s) 48, 232, 778, 908, 944, 1018
TatI WGTACW 2 cut(s) 351, 841
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 1 cut(s) 369
Tru1I TTAA 4 cut(s) 474, 741, 777, 906
Tru9I TTAA 4 cut(s) 474, 741, 777, 906
TscAI CASTG 5 cut(s) 261, 565, 646, 1053, 1200
TseFI GTSAC 1 cut(s) 997
TseI GCWGC 4 cut(s) 37, 610, 668, 860
Tsp45I GTSAC 1 cut(s) 997
TspDTI ATGAA 7 cut(s) 311, 498, 596, 691, 748, 906, 1012
TspRI CASTG 5 cut(s) 261, 565, 646, 1053, 1200
Van91I CCANNNNNTGG 1 cut(s) 60
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 4 cut(s) 48, 908, 944, 1018
XbaI TCTAGA 1 cut(s) 920
XceI RCATGY 2 cut(s) 190, 506
XmnI GAANNNNTTC 1 cut(s) 448
XspI CTAG 2 cut(s) 542, 921
Zsp2I ATGCAT 1 cut(s) 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.