MD03G1285100.v1.1
NAC Family

Dol-P-Man Man(5)GlcNAc(2)-PP-Dol

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006111
Physical Location & Seq
Reverse (-)
36500033 .. 36502095
2063 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1285100.v1.1.491

Sequence Viewer

Length: 660 bp
ATGGTTCTGCAGGTACTGTTGGGGATGCCTTTCATCATTTCTCATCCGTTTGCATACATCTCAAGAGCCTTTAATCTCGGTCGAGTTTTTATTCTCTTCTGGTCTGTTAATTTCAAGTTTGTTCCTGAGCCAATATTTGTATCTAAGGCATTTGCAGTATCTTTGCTGATTGCTCACCTTGGTTTACTCACTGCTTTTGCTCATTATAGATGGTGCATGCATGAAGGTGGACTCGTCAAATTTTTGTATTCTAGACTTGATCCTATAAAACTCAAATTTGCTCTTACAAGTTCATTCCCTTTGAAGAAGTCCTTCAATATCCATTCATCCAACAGGGTCCTTAGGAAAGAATATATTGTGACGACTATGTTTGTTGGGAATTTCATCGGTATTGTATGTGCTCGATCATTGCATTATCAGTTCTACTCGTGGTATTTCCATAGCTTACCTTATCTATTGTGGAAAACGCCTTTCCCCACAGTACTCCGTATACTGTTGTTTATCGGGGTGGAGTTTTGCTGGAATGTATTTCCTTCGAGCCTGTACTCATCGGCAATGCTTCTTTTTCTCCACCTAGTAATACTGGTCGGTCTATGGTCTGCCCCGTCTGAATATCCTTATGTGGACAACAAAGCATCGACAGAAAACAAAGATAAATGA

Protein Analysis

220

Amino Acids

25.4

Weight (kDa)

9.68

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALG3 PF05208 2 - 184 5.4e-56 ALG3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 490
AclWI GGATC 1 cut(s) 254
AcsI RAATTY 3 cut(s) 239, 275, 379
AfaI GTAC 3 cut(s) 15, 483, 545
AgsI TTSAA 3 cut(s) 115, 304, 316
AluBI AGCT 1 cut(s) 444
AluI AGCT 1 cut(s) 444
Alw21I GWGCWC 1 cut(s) 403
AlwI GGATC 1 cut(s) 254
AlwNI CAGNNNCTG 1 cut(s) 16
ApoI RAATTY 3 cut(s) 239, 275, 379
Asp700I GAANNNNTTC 1 cut(s) 311
AspS9I GGNCC 1 cut(s) 337
AsuHPI GGTGA 1 cut(s) 167
AvaII GGWCC 1 cut(s) 337
AxyI CCTNAGG 1 cut(s) 341
BauI CACGAG 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 403
BccI CCATC 1 cut(s) 204
BfaI CTAG 2 cut(s) 252, 575
BfmI CTRYAG 1 cut(s) 8
BmcAI AGTACT 1 cut(s) 483
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 338
BmsI GCATC 2 cut(s) 15, 644
Bpu10I CCTNAGC 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 46
BsaJI CCNNGG 1 cut(s) 178
Bse1I ACTGG 1 cut(s) 588
Bse21I CCTNAGG 1 cut(s) 341
Bse3DI GCAATG 2 cut(s) 407, 561
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 3 cut(s) 30, 43, 326
BseMI GCAATG 2 cut(s) 407, 561
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 1 cut(s) 588
Bsh1285I CGRYCG 1 cut(s) 82
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 403
Bsp1286I GDGCHC 1 cut(s) 403
Bsp143I GATC 2 cut(s) 259, 404
BspCNI CTCAG 1 cut(s) 118
BspLI GGNNCC 1 cut(s) 338
BspMAI CTGCAG 1 cut(s) 12
BspPI GGATC 1 cut(s) 254
BsrDI GCAATG 2 cut(s) 407, 561
BsrI ACTGG 1 cut(s) 588
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 2 cut(s) 259, 404
BssNAI GTATAC 1 cut(s) 491
BssSI CACGAG 1 cut(s) 427
BssT1I CCWWGG 1 cut(s) 178
Bst1107I GTATAC 1 cut(s) 491
Bst2BI CACGAG 1 cut(s) 427
Bst4CI ACNGT 3 cut(s) 18, 481, 495
Bst6I CTCTTC 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 3 cut(s) 126, 144, 341
BstF5I GGATG 3 cut(s) 30, 43, 326
BstKTI GATC 2 cut(s) 262, 407
BstMBI GATC 2 cut(s) 259, 404
BstMCI CGRYCG 1 cut(s) 82
BstNSI RCATGY 1 cut(s) 220
BstSFI CTRYAG 1 cut(s) 8
BstZ17I GTATAC 1 cut(s) 491
Bsu36I CCTNAGG 1 cut(s) 341
BtsCI GGATG 3 cut(s) 30, 43, 326
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 218
CaiI CAGNNNCTG 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 337
Csp6I GTAC 3 cut(s) 14, 482, 544
CviAII CATG 2 cut(s) 217, 221
CviJI RGCY 4 cut(s) 68, 130, 444, 540
CviKI_1 RGCY 4 cut(s) 68, 130, 444, 540
CviQI GTAC 3 cut(s) 14, 482, 544
DdeI CTNAG 3 cut(s) 126, 144, 341
DpnI GATC 2 cut(s) 261, 406
DpnII GATC 2 cut(s) 259, 404
Eam1104I CTCTTC 1 cut(s) 101
EarI CTCTTC 1 cut(s) 101
Eco130I CCWWGG 1 cut(s) 178
Eco47I GGWCC 1 cut(s) 337
Eco81I CCTNAGG 1 cut(s) 341
EcoO109I RGGNCCY 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 178
EcoT22I ATGCAT 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 178
FaeI CATG 2 cut(s) 220, 224
FalI AAGNNNNNCTT 2 cut(s) 296, 328
FatI CATG 2 cut(s) 216, 220
FblI GTMKAC 1 cut(s) 490
FokI GGATG 3 cut(s) 30, 37, 313
FspBI CTAG 2 cut(s) 252, 575
Hin1II CATG 2 cut(s) 220, 224
HinfI GANTC 1 cut(s) 231
HphI GGTGA 1 cut(s) 167
Hpy166II GTNNAC 4 cut(s) 185, 230, 491, 625
Hpy188I TCNGA 1 cut(s) 610
Hpy188III TCNNGA 3 cut(s) 63, 125, 252
Hpy8I GTNNAC 4 cut(s) 185, 230, 491, 625
HpyAV CCTTC 3 cut(s) 218, 322, 543
HpyCH4III ACNGT 3 cut(s) 18, 481, 495
HpyCH4V TGCA 6 cut(s) 10, 53, 155, 216, 220, 412
HpyF3I CTNAG 3 cut(s) 126, 144, 341
Hsp92II CATG 2 cut(s) 220, 224
Kzo9I GATC 2 cut(s) 259, 404
LpnPI CCDG 6 cut(s) 85, 138, 319, 505, 554, 569
LweI GCATC 2 cut(s) 15, 644
MaeI CTAG 2 cut(s) 252, 575
MaeIII GTNAC 1 cut(s) 358
MalI GATC 2 cut(s) 261, 406
MboI GATC 2 cut(s) 259, 404
MboII GAAGA 2 cut(s) 88, 316
MhlI GDGCHC 1 cut(s) 403
MluCI AATT 4 cut(s) 109, 239, 275, 379
MlyI GAGTC 1 cut(s) 225
MmeI TCCRAC 1 cut(s) 354
Mph1103I ATGCAT 1 cut(s) 222
MroXI GAANNNNTTC 1 cut(s) 311
MseI TTAA 2 cut(s) 72, 108
MslI CAYNNNNRTG 1 cut(s) 225
NdeII GATC 2 cut(s) 259, 404
NlaIII CATG 2 cut(s) 220, 224
NlaIV GGNNCC 1 cut(s) 338
NmuCI GTSAC 1 cut(s) 358
NsiI ATGCAT 1 cut(s) 222
NspI RCATGY 1 cut(s) 220
PaeI GCATGC 1 cut(s) 220
PdmI GAANNNNTTC 1 cut(s) 311
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
PpuMI RGGWCCY 1 cut(s) 337
Psp5II RGGWCCY 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 338
PspPI GGNCC 1 cut(s) 337
PspPPI RGGWCCY 1 cut(s) 337
PstI CTGCAG 1 cut(s) 12
PstNI CAGNNNCTG 1 cut(s) 16
RsaI GTAC 3 cut(s) 15, 483, 545
RsaNI GTAC 3 cut(s) 14, 482, 544
RseI CAYNNNNRTG 1 cut(s) 225
SaqAI TTAA 2 cut(s) 72, 108
Sau3AI GATC 2 cut(s) 259, 404
Sau96I GGNCC 1 cut(s) 337
ScaI AGTACT 1 cut(s) 483
SchI GAGTC 1 cut(s) 225
SduI GDGCHC 1 cut(s) 403
SetI ASST 6 cut(s) 15, 180, 229, 446, 451, 576
SfaNI GCATC 2 cut(s) 15, 644
SfcI CTRYAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SphI GCATGC 1 cut(s) 220
Sse9I AATT 4 cut(s) 109, 239, 275, 379
SspI AATATT 1 cut(s) 135
SspMI CTAG 2 cut(s) 252, 575
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 3 cut(s) 18, 481, 495
TaqI TCGA 4 cut(s) 82, 403, 536, 638
TaqII GACCGA 2 cut(s) 68, 578
TasI AATT 4 cut(s) 109, 239, 275, 379
TatI WGTACW 2 cut(s) 481, 543
Tru1I TTAA 2 cut(s) 72, 108
Tru9I TTAA 2 cut(s) 72, 108
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 5 cut(s) 22, 237, 282, 315, 373
TspGWI ACGGA 2 cut(s) 36, 476
TspRI CASTG 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 337
XapI RAATTY 3 cut(s) 239, 275, 379
XbaI TCTAGA 1 cut(s) 251
XceI RCATGY 1 cut(s) 220
XmiI GTMKAC 1 cut(s) 490
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 2 cut(s) 252, 575
ZrmI AGTACT 1 cut(s) 483
Zsp2I ATGCAT 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.