Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0002540

Physical Location & Seq

Genomic Coordinates Reverse (-)

23270899 .. 23272453

Gene Structure 1555 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g30021.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1026 bp

ATGACGTCTACTCCTGACGAAGCCTACATTAAGCGTCTCGTTCCTCTTGATTTTGGCTCAGCCAAGTCAGTTCCAGACTCTTTCGTGTGGCCTCAAGCATCCGATGAGGTCCACTCCGGTGACGGGTTAACCATACCGGTTATCGACCTCACGGATCCGAATGCCCCGCAACTCATAACCGAGGCATGTAAGACATGGGGTATTCTCCAAGTGAAAGGACATGGAATTCCAACTAAGCTTCTGGAGGACATGGAGTCTGAGTGTGAAAGACTGTTTAGTCTCCCAACTGACCAGAAAATGAAGGCCGTGAGATCTCCAGGCAGGATTACAGGATATGGTATCGCCCATATCCAGTCCTTGTTTTCCACCACTATGTGGCATGAAGGGTTTACCATCGTCGGGTCTGCAATTGATCATGCTAGGCTGCTTTGGCCTAATGATTATCAAGGTTTTTGTGACACAATAGATGATTATCAGAAGCAGATGAAGGCCTTGGCAGAGCAACTGATGCGCATCCTCTTCAAAGCCTTGAGCATTTCTGTAGAAGAACTCAATTGGCTCGATGATCCAACTAACAGCAGTGGCTGCAGTCTGTCTTTACAGCTGAACTCTTACCCTCCATGCCCCGAACCGTCCCGGACCTTAGGTCTAGCCCCCCACACAGACACCTCCATTGTAACCATACTCCAAGCTAAAACCAGCGGTCTCCAAGTCTTCAAAGACGGAGTTGGGTGGATTCTGGTGGAACTGGAACCCGATGCACTCTTCGTGAACCTTGGTGATTTCAGTCGTATTCTATCCAATGGTCAATTCATCAGTGTTCTGCATCGTGTTGTGGTGAAACCGATCCAACGCTACTCAATGGCATACTTCTTTAGGCCTCCGACGGATTTTGTTGCGTCACCACTTTTGTCGAAGGATTTGGGTCCGGGAGAAGTTCCTCGGTATCGTTCAGTCACAGTAAAGGAGTATCTTGATCTCAAGGGGAAACATCTTGAAAATGCATTATCTTTCATTGAAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009987 GO:0010033 GO:0010114 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01110 map00904 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

342

Amino Acids

37.91

Weight (kDa)

5.21

Isoelectric Point (pI)

36.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	45 - 135	1.3e-19	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	199 - 294	4.1e-27	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002540)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g30020 FvH4_2g30021 FvH4_2g30040
malus_domestica	MD08G1138400.v1.1 MD15G1116300.v1.1
prunus_persica	Prupe.1G467600_v2.0.a1 Prupe.1G467600_v2.0.a1 Prupe.1G467800_v2.0.a1
pyrus_communis	pycom08g11660 pycom15g10480
rosa_chinensis	RchiOBHm_Chr1g0337611 RchiOBHm_Chr6g0312151 RchiOBHm_Chr6g0312861
rosa_laevigata	RLG00000010291 RLG00000010350 RLG00000029265
rosa_multiflora	Rmu_co8006886.1_g000001 Rmu_co8436821.1_g000001 Rmu_sc0001215.1_g000020 Rmu_sc0008049.1_g000012
rosa_roxburghii	Rroxscaffold_4G00314130 Rroxscaffold_7G00156400 Rroxscaffold_7G00157010
rosa_rugosa	Rorug01G0136200.1 Rorug06G0393100 Rorug06G0400200
rosa_samantha	Rh1BG124500 Rh1CG145700 Rh1DG163600 Rh6AG510200 Rh6AG517400 Rh6BG521000 Rh6BG528900 Rh6CG527000 Rh6CG533500 Rh6DG512400 Rh6DG520000 Rh6DG520100
rosa_wichuraiana	Rw1G012780 Rw6G044380 Rw6G044950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	276
AatII	GACGTC	1 cut(s)	8
Acc16I	TGCGCA	1 cut(s)	512
AccB7I	CCANNNNNTGG	1 cut(s)	375
AccI	GTMKAC	1 cut(s)	8
AciI	CCGC	2 cut(s)	167, 702
AclWI	GGATC	4 cut(s)	149, 162, 560, 841
AcsI	RAATTY	1 cut(s)	225
AcyI	GRCGYC	1 cut(s)	5
AdeI	CACNNNGTG	1 cut(s)	375
AfiI	CCNNNNNNNGG	3 cut(s)	123, 375, 399
AgeI	ACCGGT	1 cut(s)	136
AgsI	TTSAA	4 cut(s)	523, 718, 998, 1019
AhdI	GACNNNNNGTC	2 cut(s)	253, 645
AjnI	CCWGG	1 cut(s)	316
AjuI	GAANNNNNNNTTGG	2 cut(s)	794, 826
AleI	CACNNNNGTG	1 cut(s)	117
AluBI	AGCT	3 cut(s)	238, 604, 692
AluI	AGCT	3 cut(s)	238, 604, 692
Alw26I	GTCTC	3 cut(s)	41, 284, 710
AlwI	GGATC	4 cut(s)	149, 162, 560, 841
AlwNI	CAGNNNCTG	1 cut(s)	585
AoxI	GGCC	5 cut(s)	89, 303, 431, 489, 878
ApeKI	GCWGC	2 cut(s)	424, 585
ApoI	RAATTY	1 cut(s)	225
AsiGI	ACCGGT	1 cut(s)	136
AspLEI	GCGC	1 cut(s)	513
AspS9I	GGNCC	3 cut(s)	109, 639, 926
AsuC2I	CCSGG	2 cut(s)	637, 930
AsuHPI	GGTGA	4 cut(s)	131, 791, 850, 894
AvaII	GGWCC	3 cut(s)	109, 639, 926
AxyI	CCTNAGG	1 cut(s)	643
BamHI	GGATCC	1 cut(s)	154
BbsI	GAAGAC	1 cut(s)	706
BbvI	GCAGC	2 cut(s)	411, 572
BccI	CCATC	1 cut(s)	401
BceAI	ACGGC	1 cut(s)	290
BciT130I	CCWGG	1 cut(s)	318
BclI	TGATCA	1 cut(s)	412
BcnI	CCSGG	2 cut(s)	637, 930
BcoDI	GTCTC	3 cut(s)	41, 284, 710
BfaI	CTAG	2 cut(s)	420, 650
BfmI	CTRYAG	2 cut(s)	540, 586
BglII	AGATCT	1 cut(s)	311
BisI	GCNGC	2 cut(s)	425, 586
BlpI	GCTNAGC	1 cut(s)	58
BlsI	GCNGC	2 cut(s)	426, 587
Bme1390I	CCNGG	3 cut(s)	318, 637, 930
Bme18I	GGWCC	3 cut(s)	109, 639, 926
BmeRI	GACNNNNNGTC	2 cut(s)	253, 645
BmgT120I	GGNCC	3 cut(s)	109, 639, 926
BmiI	GGNNCC	3 cut(s)	156, 753, 927
BmrFI	CCNGG	3 cut(s)	318, 637, 930
BmsI	GCATC	5 cut(s)	107, 498, 522, 748, 835
BpiI	GAAGAC	1 cut(s)	706
BplI	GAGNNNNNCTC	2 cut(s)	98, 130
BpmI	CTGGAG	2 cut(s)	263, 300
Bpu1102I	GCTNAGC	1 cut(s)	58
BpuEI	CTTGAG	3 cut(s)	78, 550, 965
BpuMI	CCSGG	2 cut(s)	637, 930
BsaBI	GATNNNNATC	2 cut(s)	471, 512
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	710
BsaJI	CCNNGG	4 cut(s)	180, 492, 775, 941
BsaWI	WCCGGW	2 cut(s)	116, 136
BsaXI	ACNNNNNCTCC	3 cut(s)	25, 245, 275
Bsc4I	CCNNNNNNNGG	3 cut(s)	123, 375, 399
Bse118I	RCCGGY	1 cut(s)	136
Bse1I	ACTGG	2 cut(s)	352, 753
Bse21I	CCTNAGG	1 cut(s)	643
Bse8I	GATNNNNATC	2 cut(s)	471, 512
BseBI	CCWGG	1 cut(s)	318
BseDI	CCNNGG	4 cut(s)	180, 492, 775, 941
BseGI	GGATG	2 cut(s)	98, 513
BseJI	GATNNNNATC	2 cut(s)	471, 512
BseLI	CCNNNNNNNGG	3 cut(s)	123, 375, 399
BseMII	CTCAG	2 cut(s)	72, 249
BseNI	ACTGG	2 cut(s)	352, 753
BseXI	GCAGC	2 cut(s)	411, 572
BshFI	GGCC	5 cut(s)	91, 305, 433, 491, 880
BshTI	ACCGGT	1 cut(s)	136
BsiSI	CCGG	4 cut(s)	117, 137, 637, 929
BslFI	GGGAC	1 cut(s)	619
BslI	CCNNNNNNNGG	3 cut(s)	123, 375, 399
BsmAI	GTCTC	3 cut(s)	41, 284, 710
BsmBI	CGTCTC	1 cut(s)	41
BsmFI	GGGAC	1 cut(s)	619
BsmI	GAATGC	1 cut(s)	166
BsnI	GGCC	5 cut(s)	91, 305, 433, 491, 880
Bso31I	GGTCTC	1 cut(s)	710
Bsp143I	GATC	6 cut(s)	154, 311, 412, 565, 846, 976
Bsp1720I	GCTNAGC	1 cut(s)	58
BspACI	CCGC	2 cut(s)	167, 702
BspANI	GGCC	5 cut(s)	91, 305, 433, 491, 880
BspCNI	CTCAG	2 cut(s)	71, 250
BspLI	GGNNCC	3 cut(s)	156, 753, 927
BspMAI	CTGCAG	1 cut(s)	590
BspPI	GGATC	4 cut(s)	149, 162, 560, 841
BspTNI	GGTCTC	1 cut(s)	710
BsrFI	RCCGGY	1 cut(s)	136
BsrI	ACTGG	2 cut(s)	352, 753
BssAI	RCCGGY	1 cut(s)	136
BssECI	CCNNGG	4 cut(s)	180, 492, 775, 941
BssMI	GATC	6 cut(s)	154, 311, 412, 565, 846, 976
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	2 cut(s)	492, 775
Bst2UI	CCWGG	1 cut(s)	318
Bst4CI	ACNGT	3 cut(s)	273, 633, 961
Bst6I	CTCTTC	2 cut(s)	524, 770
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	2 cut(s)	508, 585
BstDEI	CTNAG	4 cut(s)	58, 234, 258, 643
BstF5I	GGATG	2 cut(s)	98, 513
BstHHI	GCGC	1 cut(s)	513
BstKTI	GATC	6 cut(s)	157, 314, 415, 568, 849, 979
BstMAI	GTCTC	3 cut(s)	41, 284, 710
BstMBI	GATC	6 cut(s)	154, 311, 412, 565, 846, 976
BstMWI	GCNNNNNNNGC	3 cut(s)	430, 508, 585
BstNI	CCWGG	1 cut(s)	318
BstNSI	RCATGY	1 cut(s)	189
BstSCI	CCNGG	3 cut(s)	316, 635, 928
BstSFI	CTRYAG	2 cut(s)	540, 586
BstV1I	GCAGC	2 cut(s)	411, 572
BstV2I	GAAGAC	1 cut(s)	706
BstX2I	RGATCY	2 cut(s)	154, 311
BstYI	RGATCY	2 cut(s)	154, 311
Bsu36I	CCTNAGG	1 cut(s)	643
BsuRI	GGCC	5 cut(s)	91, 305, 433, 491, 880
BtsCI	GGATG	2 cut(s)	98, 513
BtsI	GCAGTG	1 cut(s)	586
BtsIMutI	CAGTG	2 cut(s)	586, 823
CaiI	CAGNNNCTG	1 cut(s)	585
CfoI	GCGC	1 cut(s)	513
Cfr10I	RCCGGY	1 cut(s)	136
Cfr13I	GGNCC	3 cut(s)	109, 639, 926
CseI	GACGC	2 cut(s)	23, 888
CspAI	ACCGGT	1 cut(s)	136
CviAII	CATG	7 cut(s)	186, 195, 221, 250, 380, 416, 621
DdeI	CTNAG	4 cut(s)	58, 234, 258, 643
DpnI	GATC	6 cut(s)	156, 313, 414, 567, 848, 978
DpnII	GATC	6 cut(s)	154, 311, 412, 565, 846, 976
DraIII	CACNNNGTG	1 cut(s)	375
DrdI	GACNNNNNNGTC	1 cut(s)	276
DriI	GACNNNNNGTC	2 cut(s)	253, 645
DseDI	GACNNNNNNGTC	1 cut(s)	276
Eam1104I	CTCTTC	2 cut(s)	524, 770
Eam1105I	GACNNNNNGTC	2 cut(s)	253, 645
EarI	CTCTTC	2 cut(s)	524, 770
Eco130I	CCWWGG	2 cut(s)	492, 775
Eco147I	AGGCCT	2 cut(s)	491, 880
Eco31I	GGTCTC	1 cut(s)	710
Eco47I	GGWCC	3 cut(s)	109, 639, 926
Eco81I	CCTNAGG	1 cut(s)	643
EcoRI	GAATTC	1 cut(s)	225
EcoRII	CCWGG	1 cut(s)	316
EcoT14I	CCWWGG	2 cut(s)	492, 775
EcoT22I	ATGCAT	1 cut(s)	1006
ErhI	CCWWGG	2 cut(s)	492, 775
Esp3I	CGTCTC	1 cut(s)	41
FaeI	CATG	7 cut(s)	189, 198, 224, 253, 383, 419, 624
FaqI	GGGAC	1 cut(s)	619
FatI	CATG	7 cut(s)	185, 194, 220, 249, 379, 415, 620
FauI	CCCGC	1 cut(s)	174
FbaI	TGATCA	1 cut(s)	412
FblI	GTMKAC	1 cut(s)	8
Fnu4HI	GCNGC	2 cut(s)	425, 586
FokI	GGATG	2 cut(s)	85, 500
Fsp4HI	GCNGC	2 cut(s)	425, 586
FspAI	RTGCGCAY	1 cut(s)	512
FspBI	CTAG	2 cut(s)	420, 650
FspI	TGCGCA	1 cut(s)	512
GlaI	GCGC	1 cut(s)	512
GluI	GCNGC	2 cut(s)	425, 586
GsuI	CTGGAG	2 cut(s)	263, 300
HaeIII	GGCC	5 cut(s)	91, 305, 433, 491, 880
HapII	CCGG	4 cut(s)	117, 137, 637, 929
HgaI	GACGC	2 cut(s)	23, 888
HhaI	GCGC	1 cut(s)	513
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	7 cut(s)	189, 198, 224, 253, 383, 419, 624
Hin6I	GCGC	1 cut(s)	511
HinP1I	GCGC	1 cut(s)	511
HincII	GTYRAC	1 cut(s)	129
HindII	GTYRAC	1 cut(s)	129
HindIII	AAGCTT	1 cut(s)	236
HinfI	GANTC	3 cut(s)	77, 254, 736
HpaI	GTTAAC	1 cut(s)	129
HpaII	CCGG	4 cut(s)	117, 137, 637, 929
HphI	GGTGA	4 cut(s)	131, 791, 850, 894
Hpy166II	GTNNAC	5 cut(s)	9, 112, 129, 390, 772
Hpy188I	TCNGA	5 cut(s)	103, 159, 259, 477, 885
Hpy188III	TCNNGA	7 cut(s)	14, 47, 74, 242, 769, 974, 995
Hpy8I	GTNNAC	5 cut(s)	9, 112, 129, 390, 772
Hpy99I	CGWCG	2 cut(s)	401, 889
HpyAV	CCTTC	4 cut(s)	295, 377, 481, 910
HpyCH4III	ACNGT	3 cut(s)	273, 633, 961
HpyCH4IV	ACGT	1 cut(s)	5
HpyCH4V	TGCA	5 cut(s)	407, 588, 761, 826, 1004
HpyF10VI	GCNNNNNNNGC	3 cut(s)	430, 508, 585
HpyF3I	CTNAG	4 cut(s)	58, 234, 258, 643
HpySE526I	ACGT	1 cut(s)	5
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	7 cut(s)	189, 198, 224, 253, 383, 419, 624
HspAI	GCGC	1 cut(s)	511
Ksp22I	TGATCA	1 cut(s)	412
KspAI	GTTAAC	1 cut(s)	129
Kzo9I	GATC	6 cut(s)	154, 311, 412, 565, 846, 976
Lsp1109I	GCAGC	2 cut(s)	411, 572
LweI	GCATC	5 cut(s)	107, 498, 522, 748, 835
MaeI	CTAG	2 cut(s)	420, 650
MaeII	ACGT	1 cut(s)	5
MaeIII	GTNAC	5 cut(s)	119, 455, 676, 900, 955
MalI	GATC	6 cut(s)	156, 313, 414, 567, 848, 978
MboI	GATC	6 cut(s)	154, 311, 412, 565, 846, 976
MboII	GAAGA	4 cut(s)	511, 557, 706, 757
MfeI	CAATTG	2 cut(s)	408, 553
MflI	RGATCY	2 cut(s)	154, 311
MluCI	AATT	5 cut(s)	225, 408, 553, 809, 1021
MlyI	GAGTC	2 cut(s)	71, 263
MmeI	TCCRAC	4 cut(s)	254, 593, 874, 908
Mph1103I	ATGCAT	1 cut(s)	1006
MseI	TTAA	3 cut(s)	30, 128, 1024
MslI	CAYNNNNRTG	2 cut(s)	117, 371
MspA1I	CMGCKG	2 cut(s)	604, 702
MspI	CCGG	4 cut(s)	117, 137, 637, 929
MspR9I	CCNGG	3 cut(s)	318, 637, 930
MunI	CAATTG	2 cut(s)	408, 553
Mva1269I	GAATGC	1 cut(s)	166
MvaI	CCWGG	1 cut(s)	318
MwoI	GCNNNNNNNGC	3 cut(s)	430, 508, 585
NciI	CCSGG	2 cut(s)	637, 930
NdeII	GATC	6 cut(s)	154, 311, 412, 565, 846, 976
NlaIII	CATG	7 cut(s)	189, 198, 224, 253, 383, 419, 624
NlaIV	GGNNCC	3 cut(s)	156, 753, 927
NmuCI	GTSAC	4 cut(s)	119, 455, 900, 955
NsbI	TGCGCA	1 cut(s)	512
NsiI	ATGCAT	1 cut(s)	1006
NspI	RCATGY	1 cut(s)	189
OliI	CACNNNNGTG	1 cut(s)	117
PceI	AGGCCT	2 cut(s)	491, 880
PctI	GAATGC	1 cut(s)	166
PfeI	GAWTC	1 cut(s)	736
PflMI	CCANNNNNTGG	1 cut(s)	375
PfoI	TCCNGGA	2 cut(s)	635, 928
PinAI	ACCGGT	1 cut(s)	136
PkrI	GCNGC	2 cut(s)	426, 587
PleI	GAGTC	2 cut(s)	71, 262
PpsI	GAGTC	2 cut(s)	71, 262
Psp6I	CCWGG	1 cut(s)	316
PspGI	CCWGG	1 cut(s)	316
PspN4I	GGNNCC	3 cut(s)	156, 753, 927
PspPI	GGNCC	3 cut(s)	109, 639, 926
PstI	CTGCAG	1 cut(s)	590
PstNI	CAGNNNCTG	1 cut(s)	585
PsuI	RGATCY	2 cut(s)	154, 311
PvuII	CAGCTG	1 cut(s)	604
RseI	CAYNNNNRTG	2 cut(s)	117, 371
SaqAI	TTAA	3 cut(s)	30, 128, 1024
SatI	GCNGC	2 cut(s)	425, 586
Sau3AI	GATC	6 cut(s)	154, 311, 412, 565, 846, 976
Sau96I	GGNCC	3 cut(s)	109, 639, 926
SchI	GAGTC	2 cut(s)	71, 263
ScrFI	CCNGG	3 cut(s)	318, 637, 930
SfaNI	GCATC	5 cut(s)	107, 498, 522, 748, 835
SfcI	CTRYAG	2 cut(s)	540, 586
SinI	GGWCC	3 cut(s)	109, 639, 926
SmiMI	CAYNNNNRTG	2 cut(s)	117, 371
SmlI	CTYRAG	3 cut(s)	93, 529, 980
SmoI	CTYRAG	3 cut(s)	93, 529, 980
Sse9I	AATT	5 cut(s)	225, 408, 553, 809, 1021
SseBI	AGGCCT	2 cut(s)	491, 880
SsiI	CCGC	2 cut(s)	167, 702
SspMI	CTAG	2 cut(s)	420, 650
StuI	AGGCCT	2 cut(s)	491, 880
StyD4I	CCNGG	3 cut(s)	316, 635, 928
StyI	CCWWGG	2 cut(s)	492, 775
TaaI	ACNGT	3 cut(s)	273, 633, 961
TaiI	ACGT	1 cut(s)	8
TaqI	TCGA	3 cut(s)	144, 561, 914
TasI	AATT	5 cut(s)	225, 408, 553, 809, 1021
TfiI	GAWTC	1 cut(s)	736
Tru1I	TTAA	3 cut(s)	30, 128, 1024
Tru9I	TTAA	3 cut(s)	30, 128, 1024
TscAI	CASTG	2 cut(s)	586, 823
TseFI	GTSAC	4 cut(s)	119, 455, 900, 955
TseI	GCWGC	2 cut(s)	424, 585
Tsp45I	GTSAC	4 cut(s)	119, 455, 900, 955
TspDTI	ATGAA	5 cut(s)	314, 396, 500, 802, 1003
TspGWI	ACGGA	3 cut(s)	167, 738, 902
TspRI	CASTG	2 cut(s)	586, 823
Van91I	CCANNNNNTGG	1 cut(s)	375
VpaK11BI	GGWCC	3 cut(s)	109, 639, 926
XapI	RAATTY	1 cut(s)	225
XceI	RCATGY	1 cut(s)	189
XmiI	GTMKAC	1 cut(s)	8
XspI	CTAG	2 cut(s)	420, 650
ZraI	GACGTC	1 cut(s)	6
Zsp2I	ATGCAT	1 cut(s)	1006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.