Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0002540

Physical Location & Seq

Genomic Coordinates Forward (+)

13258401 .. 13261683

Gene Structure 3283 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1138400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1038 bp

ATGGGTGCTATATCAGATCCCTATGGAGCTCATCTCCATCATATCATCCCCATCGACTTTAACTCAATCCAGACATTGCCTGAATCGCATGTATGGCATGATCACTCCGATGAGTCTTCATCTGGGGTACCCTCCGCCGACCCGTTATCTGTACCCGTCATCGACCTCATGGACCCGGAGGCTCCAAGTCTCATGATCCAGGCATGTGAGGAATGGGGTATTTTTCAGGTGATAGGTCATGGCCTTACAAAACTCATGGACGATGTGGAGTCTGAAGCTGAAAAATTGTTTGGTCTTCCTGTTGACCAAAAACTGAAAGCCCTACGATCTCCAGGTGGGGCTACTGGGTATGGTCGTCCTCATATATCGCCATTTTTTGACAAACATATGTGGCAGGAAGGGTTTACTATCATGGGGTCTCCAGTCGATCATGCTAAGCAACTTTGGCCCCAAGACTATCAAGGCTTTTGTGACACAATGGACGATTATCAGAACCAAATGAAGGCATTAGCACAGAAACTAATCCACATTTTCTTCAAATCTTTGGACATTAATTTTGAAGAATTTGATTGGCTTAATGACAGTGGGTCAAGCAGTCCGAGCACTGCTTTGCAACTAAACTCCTACCCGCCCTGCCCTGATCCTACTCGAGCCCTGGGCATGGCAACACACACAGACACTTCCCTGGTCACCATTGTCCAACAATCCAAAACCAGCGGCCTCCAAGTCTTCAAAGACGGAGCCGGGTGGGTTCTGGTGCAACCCATACCCGGTGCCCTAACTGTTAACCTTGGTGACTATCTCCACATTGTCTCCAATGGGGTGTTTACAAGTGTCCGTCATCGTGTTGTGGTGAACCAGCAGATCCAGCGATTTTCCATGGCCTACTTTTATTCTCCCCCAAGGGATTTTACAGTCTCCCCGGTTCTCTCTAGGGTTCTGGAAAAAGTGCCTAAGTACCGTTCACTCACAGTGAAGGAATATGTCGATATCAAGGGGAAGCATCTTGAAAAGGCATTTTCTTTGATTCAAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009987 GO:0010033 GO:0010114 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01110 map00904 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

346

Amino Acids

38.47

Weight (kDa)

5.52

Isoelectric Point (pI)

45.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	51 - 151	3.3e-17	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	203 - 300	5.7e-31	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002540)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g30020 FvH4_2g30021 FvH4_2g30040
malus_domestica	MD08G1138400.v1.1 MD15G1116300.v1.1
prunus_persica	Prupe.1G467600_v2.0.a1 Prupe.1G467600_v2.0.a1 Prupe.1G467800_v2.0.a1
pyrus_communis	pycom08g11660 pycom15g10480
rosa_chinensis	RchiOBHm_Chr1g0337611 RchiOBHm_Chr6g0312151 RchiOBHm_Chr6g0312861
rosa_laevigata	RLG00000010291 RLG00000010350 RLG00000029265
rosa_multiflora	Rmu_co8006886.1_g000001 Rmu_co8436821.1_g000001 Rmu_sc0001215.1_g000020 Rmu_sc0008049.1_g000012
rosa_roxburghii	Rroxscaffold_4G00314130 Rroxscaffold_7G00156400 Rroxscaffold_7G00157010
rosa_rugosa	Rorug01G0136200.1 Rorug06G0393100 Rorug06G0400200
rosa_samantha	Rh1BG124500 Rh1CG145700 Rh1DG163600 Rh6AG510200 Rh6AG517400 Rh6BG521000 Rh6BG528900 Rh6CG527000 Rh6CG533500 Rh6DG512400 Rh6DG520000 Rh6DG520100
rosa_wichuraiana	Rw1G012780 Rw6G044380 Rw6G044950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	127
AccB1I	GGYRCC	2 cut(s)	127, 773
AciI	CCGC	3 cut(s)	135, 629, 717
AclWI	GGATC	4 cut(s)	11, 190, 635, 859
AcsI	RAATTY	1 cut(s)	563
AcuI	CTGAAG	1 cut(s)	294
AfaI	GTAC	3 cut(s)	129, 153, 959
AfiI	CCNNNNNNNGG	3 cut(s)	502, 661, 770
AgsI	TTSAA	5 cut(s)	538, 560, 733, 1010, 1031
AhdI	GACNNNNNGTC	1 cut(s)	586
AjnI	CCWGG	4 cut(s)	198, 331, 654, 684
AjuI	GAANNNNNNNTTGG	2 cut(s)	273, 305
AluBI	AGCT	2 cut(s)	29, 278
AluI	AGCT	2 cut(s)	29, 278
Alw21I	GWGCWC	2 cut(s)	31, 605
Alw26I	GTCTC	4 cut(s)	194, 423, 817, 922
AlwI	GGATC	4 cut(s)	11, 190, 635, 859
Ama87I	CYCGRG	1 cut(s)	648
AoxI	GGCC	4 cut(s)	241, 446, 718, 882
ApoI	RAATTY	1 cut(s)	563
ArsI	GACNNNNNNTTYG	2 cut(s)	539, 571
AseI	ATTAAT	1 cut(s)	552
Asp718I	GGTACC	1 cut(s)	127
AspS9I	GGNCC	2 cut(s)	172, 447
AsuC2I	CCSGG	4 cut(s)	176, 745, 771, 923
AsuHPI	GGTGA	4 cut(s)	241, 682, 806, 865
AvaI	CYCGRG	1 cut(s)	648
AvaII	GGWCC	1 cut(s)	172
BaeGI	GKGCMC	1 cut(s)	778
BanI	GGYRCC	2 cut(s)	127, 773
BanII	GRGCYC	2 cut(s)	31, 655
BbsI	GAAGAC	3 cut(s)	108, 287, 721
Bbv12I	GWGCWC	2 cut(s)	31, 605
BccI	CCATC	2 cut(s)	45, 59
BciT130I	CCWGG	4 cut(s)	200, 333, 656, 686
BclI	TGATCA	1 cut(s)	100
BcnI	CCSGG	4 cut(s)	176, 745, 771, 923
BcoDI	GTCTC	4 cut(s)	194, 423, 817, 922
BfaI	CTAG	1 cut(s)	933
BisI	GCNGC	1 cut(s)	718
BlpI	GCTNAGC	1 cut(s)	435
BlsI	GCNGC	1 cut(s)	719
Bme1390I	CCNGG	8 cut(s)	176, 200, 333, 656, 686, 745, 771, 923
Bme18I	GGWCC	1 cut(s)	172
BmeRI	GACNNNNNGTC	1 cut(s)	586
BmeT110I	CYCGRG	1 cut(s)	648
BmgT120I	GGNCC	2 cut(s)	172, 447
BmiI	GGNNCC	6 cut(s)	129, 174, 183, 449, 742, 775
BmrFI	CCNGG	8 cut(s)	176, 200, 333, 656, 686, 745, 771, 923
BmrI	ACTGGG	1 cut(s)	354
BmsI	GCATC	1 cut(s)	1012
BmuI	ACTGGG	1 cut(s)	354
BpiI	GAAGAC	3 cut(s)	108, 287, 721
BplI	GAGNNNNNCTC	2 cut(s)	18, 50
BpmI	CTGGAG	2 cut(s)	315, 405
Bpu1102I	GCTNAGC	1 cut(s)	435
BpuMI	CCSGG	4 cut(s)	176, 745, 771, 923
BsaI	GGTCTC	1 cut(s)	423
BsaJI	CCNNGG	7 cut(s)	654, 655, 684, 790, 879, 902, 921
BsaXI	ACNNNNNCTCC	2 cut(s)	797, 827
Bsc4I	CCNNNNNNNGG	3 cut(s)	502, 661, 770
Bse1I	ACTGG	2 cut(s)	349, 422
Bse3DI	GCAATG	1 cut(s)	74
BseBI	CCWGG	4 cut(s)	200, 333, 656, 686
BseDI	CCNNGG	7 cut(s)	654, 655, 684, 790, 879, 902, 921
BseGI	GGATG	1 cut(s)	45
BseLI	CCNNNNNNNGG	3 cut(s)	502, 661, 770
BseMI	GCAATG	1 cut(s)	74
BseNI	ACTGG	2 cut(s)	349, 422
BseSI	GKGCMC	1 cut(s)	778
BshFI	GGCC	4 cut(s)	243, 448, 720, 884
BshNI	GGYRCC	2 cut(s)	127, 773
BsiHKAI	GWGCWC	2 cut(s)	31, 605
BsiHKCI	CYCGRG	1 cut(s)	648
BsiSI	CCGG	4 cut(s)	176, 744, 771, 923
BslI	CCNNNNNNNGG	3 cut(s)	502, 661, 770
BsmAI	GTCTC	4 cut(s)	194, 423, 817, 922
BsnI	GGCC	4 cut(s)	243, 448, 720, 884
Bso31I	GGTCTC	1 cut(s)	423
BsoBI	CYCGRG	1 cut(s)	648
Bsp1286I	GDGCHC	4 cut(s)	31, 605, 655, 778
Bsp143I	GATC	7 cut(s)	16, 100, 195, 326, 427, 640, 864
Bsp1720I	GCTNAGC	1 cut(s)	435
Bsp19I	CCATGG	1 cut(s)	879
BspACI	CCGC	3 cut(s)	135, 629, 717
BspANI	GGCC	4 cut(s)	243, 448, 720, 884
BspHI	TCATGA	1 cut(s)	192
BspLI	GGNNCC	6 cut(s)	129, 174, 183, 449, 742, 775
BspPI	GGATC	4 cut(s)	11, 190, 635, 859
BspT107I	GGYRCC	2 cut(s)	127, 773
BspTNI	GGTCTC	1 cut(s)	423
BsrDI	GCAATG	1 cut(s)	74
BsrI	ACTGG	2 cut(s)	349, 422
BssECI	CCNNGG	7 cut(s)	654, 655, 684, 790, 879, 902, 921
BssMI	GATC	7 cut(s)	16, 100, 195, 326, 427, 640, 864
BssT1I	CCWWGG	3 cut(s)	790, 879, 902
Bst2UI	CCWGG	4 cut(s)	200, 333, 656, 686
Bst4CI	ACNGT	5 cut(s)	584, 784, 916, 962, 973
BstDEI	CTNAG	2 cut(s)	435, 954
BstDSI	CCRYGG	1 cut(s)	879
BstEII	GGTNACC	1 cut(s)	688
BstF5I	GGATG	1 cut(s)	45
BstKTI	GATC	7 cut(s)	19, 103, 198, 329, 430, 643, 867
BstMAI	GTCTC	4 cut(s)	194, 423, 817, 922
BstMBI	GATC	7 cut(s)	16, 100, 195, 326, 427, 640, 864
BstMWI	GCNNNNNNNGC	5 cut(s)	85, 94, 445, 600, 868
BstNI	CCWGG	4 cut(s)	200, 333, 656, 686
BstNSI	RCATGY	2 cut(s)	92, 207
BstPI	GGTNACC	1 cut(s)	688
BstSCI	CCNGG	8 cut(s)	174, 198, 331, 654, 684, 743, 769, 921
BstSLI	GKGCMC	1 cut(s)	778
BstV2I	GAAGAC	3 cut(s)	108, 287, 721
BstX2I	RGATCY	2 cut(s)	16, 864
BstYI	RGATCY	2 cut(s)	16, 864
BsuRI	GGCC	4 cut(s)	243, 448, 720, 884
BtgI	CCRYGG	1 cut(s)	879
BtsCI	GGATG	1 cut(s)	45
BtsI	GCAGTG	1 cut(s)	603
BtsIMutI	CAGTG	3 cut(s)	589, 603, 978
CciI	TCATGA	1 cut(s)	192
Cfr13I	GGNCC	2 cut(s)	172, 447
Csp6I	GTAC	3 cut(s)	128, 152, 958
CviQI	GTAC	3 cut(s)	128, 152, 958
DdeI	CTNAG	2 cut(s)	435, 954
DpnI	GATC	7 cut(s)	18, 102, 197, 328, 429, 642, 866
DpnII	GATC	7 cut(s)	16, 100, 195, 326, 427, 640, 864
DriI	GACNNNNNGTC	1 cut(s)	586
Eam1105I	GACNNNNNGTC	1 cut(s)	586
EciI	GGCGGA	1 cut(s)	124
Ecl136II	GAGCTC	1 cut(s)	29
Eco130I	CCWWGG	3 cut(s)	790, 879, 902
Eco24I	GRGCYC	2 cut(s)	31, 655
Eco31I	GGTCTC	1 cut(s)	423
Eco32I	GATATC	1 cut(s)	991
Eco47I	GGWCC	1 cut(s)	172
Eco53kI	GAGCTC	1 cut(s)	29
Eco57I	CTGAAG	1 cut(s)	294
Eco88I	CYCGRG	1 cut(s)	648
Eco91I	GGTNACC	1 cut(s)	688
EcoICRI	GAGCTC	1 cut(s)	29
EcoO65I	GGTNACC	1 cut(s)	688
EcoRII	CCWGG	4 cut(s)	198, 331, 654, 684
EcoRV	GATATC	1 cut(s)	991
EcoT14I	CCWWGG	3 cut(s)	790, 879, 902
EcoT38I	GRGCYC	2 cut(s)	31, 655
ErhI	CCWWGG	3 cut(s)	790, 879, 902
FauI	CCCGC	1 cut(s)	636
FauNDI	CATATG	1 cut(s)	387
FbaI	TGATCA	1 cut(s)	100
Fnu4HI	GCNGC	1 cut(s)	718
FokI	GGATG	1 cut(s)	32
FriOI	GRGCYC	2 cut(s)	31, 655
Fsp4HI	GCNGC	1 cut(s)	718
FspBI	CTAG	1 cut(s)	933
GluI	GCNGC	1 cut(s)	718
GsuI	CTGGAG	2 cut(s)	315, 405
HaeIII	GGCC	4 cut(s)	243, 448, 720, 884
HapII	CCGG	4 cut(s)	176, 744, 771, 923
HincII	GTYRAC	2 cut(s)	304, 787
HindII	GTYRAC	2 cut(s)	304, 787
HinfI	GANTC	4 cut(s)	83, 113, 269, 1027
HpaI	GTTAAC	1 cut(s)	787
HpaII	CCGG	4 cut(s)	176, 744, 771, 923
HphI	GGTGA	4 cut(s)	241, 682, 806, 865
Hpy166II	GTNNAC	6 cut(s)	304, 405, 787, 828, 856, 965
Hpy188I	TCNGA	5 cut(s)	16, 109, 274, 492, 600
Hpy188III	TCNNGA	4 cut(s)	70, 193, 941, 1007
Hpy8I	GTNNAC	6 cut(s)	304, 405, 787, 828, 856, 965
HpyAV	CCTTC	3 cut(s)	392, 496, 970
HpyCH4III	ACNGT	5 cut(s)	584, 784, 916, 962, 973
HpyCH4V	TGCA	2 cut(s)	613, 760
HpyF10VI	GCNNNNNNNGC	5 cut(s)	85, 94, 445, 600, 868
HpyF3I	CTNAG	2 cut(s)	435, 954
KpnI	GGTACC	1 cut(s)	131
Ksp22I	TGATCA	1 cut(s)	100
KspAI	GTTAAC	1 cut(s)	787
Kzo9I	GATC	7 cut(s)	16, 100, 195, 326, 427, 640, 864
LmnI	GCTCC	3 cut(s)	26, 187, 740
LweI	GCATC	1 cut(s)	1012
MaeI	CTAG	1 cut(s)	933
MaeIII	GTNAC	3 cut(s)	470, 688, 794
MalI	GATC	7 cut(s)	18, 102, 197, 328, 429, 642, 866
MboI	GATC	7 cut(s)	16, 100, 195, 326, 427, 640, 864
MboII	GAAGA	5 cut(s)	108, 287, 526, 572, 721
MflI	RGATCY	2 cut(s)	16, 864
MhlI	GDGCHC	4 cut(s)	31, 605, 655, 778
MluCI	AATT	4 cut(s)	284, 553, 563, 1033
MlyI	GAGTC	2 cut(s)	122, 278
MmeI	TCCRAC	1 cut(s)	724
MnlI	CCTC	6 cut(s)	142, 172, 176, 202, 369, 731
MseI	TTAA	4 cut(s)	60, 552, 576, 786
MslI	CAYNNNNRTG	1 cut(s)	108
MspA1I	CMGCKG	1 cut(s)	717
MspI	CCGG	4 cut(s)	176, 744, 771, 923
MspR9I	CCNGG	8 cut(s)	176, 200, 333, 656, 686, 745, 771, 923
MvaI	CCWGG	4 cut(s)	200, 333, 656, 686
MwoI	GCNNNNNNNGC	5 cut(s)	85, 94, 445, 600, 868
NciI	CCSGG	4 cut(s)	176, 745, 771, 923
NcoI	CCATGG	1 cut(s)	879
NdeI	CATATG	1 cut(s)	387
NdeII	GATC	7 cut(s)	16, 100, 195, 326, 427, 640, 864
NlaIV	GGNNCC	6 cut(s)	129, 174, 183, 449, 742, 775
NmuCI	GTSAC	3 cut(s)	470, 688, 794
NspI	RCATGY	2 cut(s)	92, 207
PaeR7I	CTCGAG	1 cut(s)	648
PagI	TCATGA	1 cut(s)	192
PasI	CCCWGGG	1 cut(s)	655
PfeI	GAWTC	2 cut(s)	83, 1027
PkrI	GCNGC	1 cut(s)	719
PleI	GAGTC	2 cut(s)	121, 277
PpsI	GAGTC	2 cut(s)	121, 277
PshBI	ATTAAT	1 cut(s)	552
Psp124BI	GAGCTC	1 cut(s)	31
Psp6I	CCWGG	4 cut(s)	198, 331, 654, 684
PspEI	GGTNACC	1 cut(s)	688
PspGI	CCWGG	4 cut(s)	198, 331, 654, 684
PspN4I	GGNNCC	6 cut(s)	129, 174, 183, 449, 742, 775
PspPI	GGNCC	2 cut(s)	172, 447
PspXI	VCTCGAGB	1 cut(s)	648
PsuI	RGATCY	2 cut(s)	16, 864
RsaI	GTAC	3 cut(s)	129, 153, 959
RsaNI	GTAC	3 cut(s)	128, 152, 958
RseI	CAYNNNNRTG	1 cut(s)	108
SacI	GAGCTC	1 cut(s)	31
SaqAI	TTAA	4 cut(s)	60, 552, 576, 786
SatI	GCNGC	1 cut(s)	718
Sau3AI	GATC	7 cut(s)	16, 100, 195, 326, 427, 640, 864
Sau96I	GGNCC	2 cut(s)	172, 447
SchI	GAGTC	2 cut(s)	122, 278
ScrFI	CCNGG	8 cut(s)	176, 200, 333, 656, 686, 745, 771, 923
SduI	GDGCHC	4 cut(s)	31, 605, 655, 778
SetI	ASST	7 cut(s)	31, 168, 231, 238, 280, 337, 792
SfaNI	GCATC	1 cut(s)	1012
Sfr274I	CTCGAG	1 cut(s)	648
SinI	GGWCC	1 cut(s)	172
SlaI	CTCGAG	1 cut(s)	648
SmiMI	CAYNNNNRTG	1 cut(s)	108
SmlI	CTYRAG	1 cut(s)	648
SmoI	CTYRAG	1 cut(s)	648
Sse9I	AATT	4 cut(s)	284, 553, 563, 1033
SsiI	CCGC	3 cut(s)	135, 629, 717
SspMI	CTAG	1 cut(s)	933
SstI	GAGCTC	1 cut(s)	31
StyD4I	CCNGG	8 cut(s)	174, 198, 331, 654, 684, 743, 769, 921
StyI	CCWWGG	3 cut(s)	790, 879, 902
TaaI	ACNGT	5 cut(s)	584, 784, 916, 962, 973
TaqI	TCGA	5 cut(s)	54, 162, 426, 649, 987
TasI	AATT	4 cut(s)	284, 553, 563, 1033
TauI	GCSGC	1 cut(s)	720
TfiI	GAWTC	2 cut(s)	83, 1027
Tru1I	TTAA	4 cut(s)	60, 552, 576, 786
Tru9I	TTAA	4 cut(s)	60, 552, 576, 786
TscAI	CASTG	3 cut(s)	589, 610, 978
TseFI	GTSAC	3 cut(s)	470, 688, 794
Tsp45I	GTSAC	3 cut(s)	470, 688, 794
TspDTI	ATGAA	2 cut(s)	108, 515
TspGWI	ACGGA	2 cut(s)	753, 827
TspRI	CASTG	3 cut(s)	589, 610, 978
VpaK11BI	GGWCC	1 cut(s)	172
VspI	ATTAAT	1 cut(s)	552
XapI	RAATTY	1 cut(s)	563
XceI	RCATGY	2 cut(s)	92, 207
XhoI	CTCGAG	1 cut(s)	648
XspI	CTAG	1 cut(s)	933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.