FvH4_2g34270

Protein ROOT INITIATION DEFECTIVE 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007848
Physical Location & Seq
Forward (+)
25500792 .. 25503798
3007 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34270.t2

Sequence Viewer

Length: 1335 bp
ATGGAGGTGGTGGTGGCCTCCTCCTCCGTCGAATCCGACATCGCCTGCTGGGACTTCAACACCGGCGCCGAGCTCCTCCGCTACAAGTCCTGCGCCTCTCCCAATCACGGCCTCGTCTCCGTCGGCGACCGTTTCCTCGCATCCTCTCAGCTCCGTCACACCAGTGACGGCGGCTCCGTCAAGTATTGGGCCTGGTCCAAACCTCAGCCTGATGCCAAGAGCTCGCAAGACGAACCGATAAAACCGCTGGCTGCTAACAGAGAGGGCACCTACGTGGTTGGTGGAGGTGTCTCCGGGACTATCTACTTGTGGGAGGTAACTAGCGGTAGGCTGCTTAGGAAGTGGGATGCTCACTATAGAGCTGTAAGTTGCTTGAGATTTTCTGATGACGGCACCATACTGTTTTCGGGGTCTGAGGATGGAGAAATTAGAGCCTGGAACCTTGTAACGATATTCGATGATCATCAAAGTCAACATGAAAAGTATGGACACTTGCTTAGTTTTACCGAGCACTCGCTTTCTGTAACGGATATGGTGTTAGGATTTGGATATGATCCCGTTATTGTGTCGGCTTCAGAGGATCGAACATGCAAGATTTGGAGCTTTTCAAGAGGAACATTACTGAGAAATATTGTTTTTCCGACAATAATTGATGCAATTGCAATGGATCCTGGTGAAAATCTCCTCTTTGCTGGTGGTAGAGATGGAAATATATACGTGGCTGCACTTAATACTGCAAAGACAGCTAGCAGCAAATATGGGTTGCATATCATCAATTGCTTCTCAAACCACAGCAAGGCAGTTACTTGCTTGGCATGGGGACTATCTGGAAACTATTTGATTTCTGGATCAGAGGATGGCATGGTTAGAGTATGGGATGCTAAAACTGCTAACATTATCCGCGTGCTCAAACACTCAAAAGGTCCAGTGAATAATATTCTAGTTATTAGACAACAAGTTTCTCATACGATCCCGAATTCACAAGCCTCCTCCAGGCGGCACGGTTCCTCATTACCACCTCCACTAGAAAAGCGTGCATATGAAGCAGATAAAGATGCAGGTTTTAAGGCAGTCATCGGTCTCCAGGGTACTTGCAATAAATCCACAGATGCAGTGTATATCACTTCTGACGTGATGGAGTGTCAAATCAGAGAGCTTCAGCAACAAGGTTCTGCTTCAGCTGAAATGGAGATTGAGAGACTGAAGCATGAGAATAAAAGATGCCAACAAATGATTCAGCAGTGGAAGAAAATGTATGACAATTTGCACCAATTCTGTGTAAATGAGCTTGTCGATGGTGATAAAGGAGGAGCCCCAAATGGAAATGCTCCCTAA

Protein Analysis

445

Amino Acids

48.82

Weight (kDa)

6.4

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 4 - 149 6.5e-17 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 5 - 154 1.3e-11 THOC3 beta-propeller domain
WDR55 PF24796 6 - 150 2e-06 WDR55
Beta-prop_EML_2 PF23414 54 - 176 8.2e-10 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_EML PF23409 75 - 148 1.7e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st PF25173 75 - 240 3.3e-19 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 76 - 204 5.5e-09 CDC20/Fizzy WD40 domain
WD40_Gbeta PF25391 86 - 304 1.9e-08 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 90 - 182 5.1e-08 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 92 - 250 1.4e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 95 - 202 1.8e-06 WDR36/Utp21 first beta-propeller
NBCH_WD40 PF20426 100 - 251 6.9e-06 Neurobeachin beta propeller domain
WD40 PF00400 110 - 147 1.6e-08 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 117 - 313 5.3e-18 WDHD1 first WD40 domain
EIF3I PF24805 118 - 305 5.4e-14 EIF3I
Beta-prop_THOC3 PF25174 122 - 252 6.8e-11 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 132 - 312 1.5e-10 TEP-1 second beta-propeller
Beta-prop_RIG_2nd PF23775 149 - 305 4.6e-08 RIG second beta-propeller
Beta-prop_WDR5 PF25175 165 - 307 1.4e-17 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 165 - 294 2.9e-09 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 184 - 305 3.8e-10 WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz PF24807 185 - 302 3e-11 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 186 - 308 1.9e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 187 - 307 1.3e-12 THOC3 beta-propeller domain
WDR55 PF24796 210 - 315 1.4e-07 WDR55
Beta-prop_HPS5 PF23756 216 - 304 6.2e-08 HPS5 beta-propeller
Beta-prop_WDR3_1st PF25173 217 - 307 4.2e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 217 - 308 1.3e-09 Prp19 WD40 domain
WD40 PF00400 258 - 293 5.5e-10 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1049
AccB1I GGYRCC 3 cut(s) 65, 266, 392
AccII CGCG 1 cut(s) 903
AciI CCGC 6 cut(s) 79, 171, 245, 324, 901, 997
AclWI GGATC 6 cut(s) 548, 588, 662, 675, 856, 964
AcsI RAATTY 1 cut(s) 976
AcuI CTGAAG 4 cut(s) 558, 1142, 1161, 1223
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 1 cut(s) 1090
AfiI CCNNNNNNNGG 4 cut(s) 107, 796, 993, 996
AgsI TTSAA 2 cut(s) 58, 609
AjiI CACGTC 1 cut(s) 1132
AjnI CCWGG 5 cut(s) 191, 434, 670, 992, 1083
AjuI GAANNNNNNNTTGG 2 cut(s) 1218, 1250
AleI CACNNNNGTG 2 cut(s) 162, 272
AluBI AGCT 9 cut(s) 73, 151, 222, 362, 603, 746, 1156, 1181, 1288
AluI AGCT 9 cut(s) 73, 151, 222, 362, 603, 746, 1156, 1181, 1288
Alw21I GWGCWC 4 cut(s) 75, 224, 513, 909
Alw26I GTCTC 4 cut(s) 121, 295, 1085, 1192
AlwI GGATC 6 cut(s) 548, 588, 662, 675, 856, 964
AoxI GGCC 3 cut(s) 15, 109, 189
ApeKI GCWGC 4 cut(s) 251, 331, 722, 750
ApoI RAATTY 1 cut(s) 976
AspLEI GCGC 2 cut(s) 68, 95
AspS9I GGNCC 3 cut(s) 189, 195, 923
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 2 cut(s) 686, 1310
AsuNHI GCTAGC 1 cut(s) 746
AvaII GGWCC 2 cut(s) 195, 923
BaeGI GKGCMC 1 cut(s) 269
BamHI GGATCC 1 cut(s) 667
BanI GGYRCC 3 cut(s) 65, 266, 392
BanII GRGCYC 3 cut(s) 75, 224, 1315
Bbv12I GWGCWC 4 cut(s) 75, 224, 513, 909
BbvCI CCTCAGC 1 cut(s) 204
BbvI GCAGC 4 cut(s) 238, 318, 709, 762
BccI CCATC 5 cut(s) 413, 698, 851, 1129, 1289
BceAI ACGGC 3 cut(s) 124, 184, 406
BciT130I CCWGG 5 cut(s) 193, 436, 672, 994, 1085
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 1 cut(s) 295
BcoDI GTCTC 4 cut(s) 121, 295, 1085, 1192
BfaI CTAG 4 cut(s) 321, 747, 941, 1025
BfmI CTRYAG 1 cut(s) 355
BfoI RGCGCY 1 cut(s) 69
BfuAI ACCTGC 1 cut(s) 1049
BisI GCNGC 6 cut(s) 172, 252, 332, 723, 751, 998
BlsI GCNGC 6 cut(s) 173, 253, 333, 724, 752, 999
Bme1390I CCNGG 6 cut(s) 193, 295, 436, 672, 994, 1085
Bme18I GGWCC 2 cut(s) 195, 923
BmgBI CACGTC 1 cut(s) 1132
BmgT120I GGNCC 3 cut(s) 189, 195, 923
BmiI GGNNCC 8 cut(s) 67, 175, 268, 394, 440, 669, 1006, 1312
BmrFI CCNGG 6 cut(s) 193, 295, 436, 672, 994, 1085
BmsI GCATC 8 cut(s) 149, 202, 337, 643, 868, 1045, 1099, 1211
BmtI GCTAGC 1 cut(s) 750
BpmI CTGGAG 2 cut(s) 976, 1067
Bpu10I CCTNAGC 2 cut(s) 204, 335
BpuEI CTTGAG 1 cut(s) 394
BpuMI CCSGG 1 cut(s) 295
BsaAI YACGTR 2 cut(s) 274, 718
BsaBI GATNNNNATC 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 1085
BsaJI CCNNGG 1 cut(s) 1084
BsaXI ACNNNNNCTCC 2 cut(s) 158, 188
Bsc4I CCNNNNNNNGG 4 cut(s) 107, 796, 993, 996
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 162, 926
Bse3DI GCAATG 1 cut(s) 669
Bse8I GATNNNNATC 1 cut(s) 462
BseBI CCWGG 5 cut(s) 193, 436, 672, 994, 1085
BseDI CCNNGG 1 cut(s) 1084
BseGI GGATG 5 cut(s) 140, 352, 424, 862, 883
BseJI GATNNNNATC 1 cut(s) 462
BseLI CCNNNNNNNGG 4 cut(s) 107, 796, 993, 996
BseMI GCAATG 1 cut(s) 669
BseMII CTCAG 4 cut(s) 161, 218, 405, 614
BseNI ACTGG 2 cut(s) 162, 926
BseRI GAGGAG 6 cut(s) 10, 13, 65, 674, 979, 1323
BseSI GKGCMC 1 cut(s) 269
BseXI GCAGC 4 cut(s) 238, 318, 709, 762
BseYI CCCAGC 1 cut(s) 48
BsgI GTGCAG 1 cut(s) 708
Bsh1236I CGCG 1 cut(s) 903
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 3 cut(s) 17, 111, 191
BshNI GGYRCC 3 cut(s) 65, 266, 392
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 4 cut(s) 75, 224, 513, 909
BsiSI CCGG 2 cut(s) 63, 294
BslFI GGGAC 3 cut(s) 65, 310, 834
BslI CCNNNNNNNGG 4 cut(s) 107, 796, 993, 996
BsmAI GTCTC 4 cut(s) 121, 295, 1085, 1192
BsmBI CGTCTC 1 cut(s) 121
BsmFI GGGAC 3 cut(s) 65, 310, 834
BsnI GGCC 3 cut(s) 17, 111, 191
Bso31I GGTCTC 1 cut(s) 1085
Bsp1286I GDGCHC 6 cut(s) 75, 224, 269, 513, 909, 1315
Bsp143I GATC 6 cut(s) 460, 553, 580, 667, 848, 969
BspACI CCGC 6 cut(s) 79, 171, 245, 324, 901, 997
BspANI GGCC 3 cut(s) 17, 111, 191
BspCNI CTCAG 4 cut(s) 160, 217, 406, 615
BspFNI CGCG 1 cut(s) 903
BspLI GGNNCC 8 cut(s) 67, 175, 268, 394, 440, 669, 1006, 1312
BspMI ACCTGC 1 cut(s) 1049
BspOI GCTAGC 1 cut(s) 750
BspPI GGATC 6 cut(s) 548, 588, 662, 675, 856, 964
BspT107I GGYRCC 3 cut(s) 65, 266, 392
BspTNI GGTCTC 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 669
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 2 cut(s) 162, 926
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 1 cut(s) 1084
BssMI GATC 6 cut(s) 460, 553, 580, 667, 848, 969
BssNI GRCGYC 1 cut(s) 66
Bst2UI CCWGG 5 cut(s) 193, 436, 672, 994, 1085
Bst4CI ACNGT 3 cut(s) 131, 402, 1004
BstACI GRCGYC 1 cut(s) 66
BstBAI YACGTR 2 cut(s) 274, 718
BstC8I GCNNGC 6 cut(s) 46, 224, 249, 748, 905, 1035
BstDEI CTNAG 6 cut(s) 147, 204, 335, 414, 497, 623
BstENI CCTNNNNNAGG 1 cut(s) 991
BstF5I GGATG 5 cut(s) 140, 352, 424, 862, 883
BstFNI CGCG 1 cut(s) 903
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 2 cut(s) 68, 95
BstKTI GATC 6 cut(s) 463, 556, 583, 670, 851, 972
BstMAI GTCTC 4 cut(s) 121, 295, 1085, 1192
BstMBI GATC 6 cut(s) 460, 553, 580, 667, 848, 969
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 3 cut(s) 743, 887, 1043
BstNI CCWGG 5 cut(s) 193, 436, 672, 994, 1085
BstNSI RCATGY 1 cut(s) 591
BstSCI CCNGG 6 cut(s) 191, 293, 434, 670, 992, 1083
BstSFI CTRYAG 1 cut(s) 355
BstSLI GKGCMC 1 cut(s) 269
BstUI CGCG 1 cut(s) 903
BstV1I GCAGC 4 cut(s) 238, 318, 709, 762
BstX2I RGATCY 1 cut(s) 667
BstYI RGATCY 1 cut(s) 667
BsuRI GGCC 3 cut(s) 17, 111, 191
BtgZI GCGATG 1 cut(s) 25
BtrI CACGTC 1 cut(s) 1132
BtsCI GGATG 5 cut(s) 140, 352, 424, 862, 883
BtsI GCAGTG 2 cut(s) 1119, 1247
BtsIMutI CAGTG 4 cut(s) 169, 933, 1119, 1247
BveI ACCTGC 1 cut(s) 1049
Cac8I GCNNGC 6 cut(s) 46, 224, 249, 748, 905, 1035
CfoI GCGC 2 cut(s) 68, 95
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 3 cut(s) 189, 195, 923
Csp6I GTAC 1 cut(s) 1089
CviAII CATG 5 cut(s) 476, 588, 816, 862, 1208
CviQI GTAC 1 cut(s) 1089
DdeI CTNAG 6 cut(s) 147, 204, 335, 414, 497, 623
DinI GGCGCC 1 cut(s) 67
DpnI GATC 6 cut(s) 462, 555, 582, 669, 850, 971
DpnII GATC 6 cut(s) 460, 553, 580, 667, 848, 969
Ecl136II GAGCTC 2 cut(s) 73, 222
Eco24I GRGCYC 3 cut(s) 75, 224, 1315
Eco31I GGTCTC 1 cut(s) 1085
Eco47I GGWCC 2 cut(s) 195, 923
Eco53kI GAGCTC 2 cut(s) 73, 222
Eco57I CTGAAG 4 cut(s) 558, 1142, 1161, 1223
EcoICRI GAGCTC 2 cut(s) 73, 222
EcoNI CCTNNNNNAGG 1 cut(s) 991
EcoRI GAATTC 1 cut(s) 976
EcoRII CCWGG 5 cut(s) 191, 434, 670, 992, 1083
EcoT38I GRGCYC 3 cut(s) 75, 224, 1315
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
Esp3I CGTCTC 1 cut(s) 121
FaeI CATG 5 cut(s) 479, 591, 819, 865, 1211
FaqI GGGAC 3 cut(s) 65, 310, 834
FatI CATG 5 cut(s) 475, 587, 815, 861, 1207
FauNDI CATATG 1 cut(s) 1039
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 6 cut(s) 172, 252, 332, 723, 751, 998
FokI GGATG 5 cut(s) 127, 359, 431, 869, 890
FriOI GRGCYC 3 cut(s) 75, 224, 1315
Fsp4HI GCNGC 6 cut(s) 172, 252, 332, 723, 751, 998
FspBI CTAG 4 cut(s) 321, 747, 941, 1025
GlaI GCGC 2 cut(s) 67, 94
GluI GCNGC 6 cut(s) 172, 252, 332, 723, 751, 998
GsaI CCCAGC 1 cut(s) 52
GsuI CTGGAG 2 cut(s) 976, 1067
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 3 cut(s) 17, 111, 191
HapII CCGG 2 cut(s) 63, 294
HhaI GCGC 2 cut(s) 68, 95
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 5 cut(s) 479, 591, 819, 865, 1211
Hin6I GCGC 2 cut(s) 66, 93
HinP1I GCGC 2 cut(s) 66, 93
HincII GTYRAC 1 cut(s) 473
HindII GTYRAC 1 cut(s) 473
HinfI GANTC 2 cut(s) 32, 1234
HpaII CCGG 2 cut(s) 63, 294
HphI GGTGA 2 cut(s) 686, 1310
Hpy166II GTNNAC 1 cut(s) 473
Hpy188I TCNGA 8 cut(s) 37, 385, 415, 577, 642, 853, 1129, 1151
Hpy188III TCNNGA 4 cut(s) 609, 828, 846, 973
Hpy8I GTNNAC 1 cut(s) 473
Hpy99I CGWCG 2 cut(s) 32, 125
HpyCH4III ACNGT 3 cut(s) 131, 402, 1004
HpyCH4IV ACGT 3 cut(s) 273, 717, 1131
HpyF10VI GCNNNNNNNGC 3 cut(s) 743, 887, 1043
HpyF3I CTNAG 6 cut(s) 147, 204, 335, 414, 497, 623
HpySE526I ACGT 3 cut(s) 273, 717, 1131
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 5 cut(s) 479, 591, 819, 865, 1211
HspAI GCGC 2 cut(s) 66, 93
KasI GGCGCC 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 6 cut(s) 460, 553, 580, 667, 848, 969
LmnI GCTCC 6 cut(s) 78, 156, 179, 600, 1310, 1333
Lsp1109I GCAGC 4 cut(s) 238, 318, 709, 762
LweI GCATC 8 cut(s) 149, 202, 337, 643, 868, 1045, 1099, 1211
MaeI CTAG 4 cut(s) 321, 747, 941, 1025
MaeII ACGT 3 cut(s) 273, 717, 1131
MaeIII GTNAC 6 cut(s) 155, 164, 316, 445, 523, 802
MalI GATC 6 cut(s) 462, 555, 582, 669, 850, 971
MboI GATC 6 cut(s) 460, 553, 580, 667, 848, 969
MboII GAAGA 1 cut(s) 1258
MfeI CAATTG 2 cut(s) 657, 775
MflI RGATCY 1 cut(s) 667
MhlI GDGCHC 6 cut(s) 75, 224, 269, 513, 909, 1315
MluCI AATT 7 cut(s) 426, 648, 657, 775, 976, 1261, 1271
Mly113I GGCGCC 1 cut(s) 66
MmeI TCCRAC 2 cut(s) 60, 665
MseI TTAA 2 cut(s) 729, 1065
MslI CAYNNNNRTG 2 cut(s) 162, 272
MspA1I CMGCKG 2 cut(s) 247, 1181
MspI CCGG 2 cut(s) 63, 294
MspR9I CCNGG 6 cut(s) 193, 295, 436, 672, 994, 1085
MunI CAATTG 2 cut(s) 657, 775
MvaI CCWGG 5 cut(s) 193, 436, 672, 994, 1085
MvnI CGCG 1 cut(s) 903
MwoI GCNNNNNNNGC 3 cut(s) 743, 887, 1043
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 1 cut(s) 295
NdeI CATATG 1 cut(s) 1039
NdeII GATC 6 cut(s) 460, 553, 580, 667, 848, 969
NheI GCTAGC 1 cut(s) 746
NlaIII CATG 5 cut(s) 479, 591, 819, 865, 1211
NlaIV GGNNCC 8 cut(s) 67, 175, 268, 394, 440, 669, 1006, 1312
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 2 cut(s) 155, 164
NspI RCATGY 1 cut(s) 591
OliI CACNNNNGTG 2 cut(s) 162, 272
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PfeI GAWTC 2 cut(s) 32, 1234
PfoI TCCNGGA 1 cut(s) 293
PkrI GCNGC 6 cut(s) 173, 253, 333, 724, 752, 999
PluTI GGCGCC 1 cut(s) 69
Ppu21I YACGTR 2 cut(s) 274, 718
Psp124BI GAGCTC 2 cut(s) 75, 224
Psp6I CCWGG 5 cut(s) 191, 434, 670, 992, 1083
PspFI CCCAGC 1 cut(s) 48
PspGI CCWGG 5 cut(s) 191, 434, 670, 992, 1083
PspN4I GGNNCC 8 cut(s) 67, 175, 268, 394, 440, 669, 1006, 1312
PspPI GGNCC 3 cut(s) 189, 195, 923
PsuI RGATCY 1 cut(s) 667
PvuII CAGCTG 1 cut(s) 1181
RsaI GTAC 1 cut(s) 1090
RsaNI GTAC 1 cut(s) 1089
RseI CAYNNNNRTG 2 cut(s) 162, 272
SacI GAGCTC 2 cut(s) 75, 224
SaqAI TTAA 2 cut(s) 729, 1065
SatI GCNGC 6 cut(s) 172, 252, 332, 723, 751, 998
Sau3AI GATC 6 cut(s) 460, 553, 580, 667, 848, 969
Sau96I GGNCC 3 cut(s) 189, 195, 923
ScrFI CCNGG 6 cut(s) 193, 295, 436, 672, 994, 1085
SduI GDGCHC 6 cut(s) 75, 224, 269, 513, 909, 1315
SfaNI GCATC 8 cut(s) 149, 202, 337, 643, 868, 1045, 1099, 1211
SfcI CTRYAG 1 cut(s) 355
SfoI GGCGCC 1 cut(s) 67
SgrAI CRCCGGYG 1 cut(s) 62
SinI GGWCC 2 cut(s) 195, 923
SmiMI CAYNNNNRTG 2 cut(s) 162, 272
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 7 cut(s) 426, 648, 657, 775, 976, 1261, 1271
SsiI CCGC 6 cut(s) 79, 171, 245, 324, 901, 997
SspDI GGCGCC 1 cut(s) 65
SspI AATATT 2 cut(s) 631, 937
SspMI CTAG 4 cut(s) 321, 747, 941, 1025
SstI GAGCTC 2 cut(s) 75, 224
StyD4I CCNGG 6 cut(s) 191, 293, 434, 670, 992, 1083
TaaI ACNGT 3 cut(s) 131, 402, 1004
TaiI ACGT 3 cut(s) 276, 720, 1134
TaqI TCGA 4 cut(s) 30, 456, 583, 1293
TaqII GACCGA 1 cut(s) 1067
TasI AATT 7 cut(s) 426, 648, 657, 775, 976, 1261, 1271
TauI GCSGC 2 cut(s) 174, 1000
TfiI GAWTC 2 cut(s) 32, 1234
Tru1I TTAA 2 cut(s) 729, 1065
Tru9I TTAA 2 cut(s) 729, 1065
TscAI CASTG 4 cut(s) 169, 933, 1119, 1247
TseFI GTSAC 2 cut(s) 155, 164
TseI GCWGC 4 cut(s) 251, 331, 722, 750
Tsp45I GTSAC 2 cut(s) 155, 164
TspDTI ATGAA 2 cut(s) 492, 1056
TspGWI ACGGA 5 cut(s) 16, 109, 143, 166, 542
TspRI CASTG 4 cut(s) 169, 933, 1119, 1247
VpaK11BI GGWCC 2 cut(s) 195, 923
XagI CCTNNNNNAGG 1 cut(s) 991
XapI RAATTY 1 cut(s) 976
XceI RCATGY 1 cut(s) 591
XspI CTAG 4 cut(s) 321, 747, 941, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.