MD15G1081000.v1.1

Protein ROOT INITIATION DEFECTIVE 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007848
Physical Location & Seq
Forward (+)
5542707 .. 5546126
3420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1081000.v1.1.491

Sequence Viewer

Length: 1326 bp
ATGGAGCTGGTGGTGGCCTCTTCGTCTGTCGAAGCCGGCATCGGCTGTTGGGACCTCCTCAACGGCCCCGAGCAGCTCCGCTACAAGCTCTGCGCTTCTCCGCCTCATGGCCTCATTTGCGTCGGCGAACGATTCCTCGCCTCCTCTCAGCTCCGTGAGCCTTCCGCCTCCTCTGGCTCTGTCCAGTACTGGTCTTGGTCCAAGCCGCAGGTTGATGTGAAGAGCTTCCCAGAAGAACCGATCAATCCGCTCGCTGCGAACAGCGAAGGAACCTACATTGCTGGCGGTGGCTCATCGGGGAATATCTACTTGTGGGAGGTTAATACTGGTAGACTGCTTAAGAAATGGCATGCTCACTACAGAGGTGTTAGTTGCTTGGCATTTTCGGATGATGGCTCCCTGCTGATTTCGGGGTCAGAGGATGGAGGCATTAGAGTTTGGTCTCTTATAAGATTATTCGATGATTATCAAAGTCAACAGGAAAGCCATCTGTACATACATAATTTTACGGAGCACACTCTTTGTGTAACGGATGTTGTCACAGGATATGGTGGATGCAAAGCCATTATCGTGTCAGCATCACAGGATCGCACATGTAAGGTTTGGAGTGTAGGAAGTGGGAGATTATTGAGAAATATTGTTTTCCCTACTATAATTGATGCAATTGCACTGGATCCTGGTGAAAATGTCTTCTTTGCCGGTGGTAGAGATGGCAAGATATACGTTGCTGCACTTAATGCTGAGAGCCCACTAAACAGCAAGTATGGGTTGCATATCATCGATTGTTTCTCGAATCACAGCAAAGCTGTTACTTGCCTAGCATATGGCATTACTGGAAATTATTTGCTTTCTGGATCAGAAGATGGCGTCATTCGAGTTTGGGATGCTAGAACTCTTAACATTGTTCGTGTGTTCAAACATGCAAAAGGTCCAGTGAATAATATTCTAGTCGTTCGACAGCATTTTTCTCAGATGAATTCATATGCGTCCTCGAGAAGGCATGGTTCATCATTACCACCTCCACTGGAAAAGTATACAACAGAGGAAGGCGGAGACATTAGAGCTTTCCTTGGAGGCCCGGCTACTTGCAGTAATTTGACAGAACCATTGTACATCACTGAACAAGTGATGAATAGGCAAATCAAAGAGCTTCAGCAACAAGGTTCTGCCTCAGCCGAAATGGAGATTGAGAGACTCAAGCGGGAAAATGAAAGAGCCACACAAATGGCTCAGCAGTGGAGAAAAATGTATGACAACTTGCATCAGTTCTGTGTAAATGAGCTTTTGGATGGTGATCAAGGTGTCTCAAATGGAAATGCAGCTTGA

Protein Analysis

442

Amino Acids

48.47

Weight (kDa)

5.97

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 54 - 180 2.1e-07 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 56 - 149 1.2e-10 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 58 - 166 6.2e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 62 - 204 3.4e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 73 - 242 3.9e-20 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 78 - 151 6.9e-06 WDR36/Utp21 first beta-propeller
Beta-prop_TEP1_2nd PF25047 78 - 204 2.8e-07 TEP-1 second beta-propeller
WD40_Prp19 PF24814 80 - 149 2.2e-09 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 81 - 148 5e-13 WDR5 beta-propeller domain
Beta-prop_EML PF23409 84 - 164 2.9e-08 Echinoderm microtubule-associated protein first beta-propeller
NBCH_WD40 PF20426 84 - 315 2.5e-15 Neurobeachin beta propeller domain
Beta-prop_CAF1B_HIR1 PF24105 88 - 237 7.2e-07 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 111 - 148 3.5e-10 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 117 - 240 5.4e-06 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 118 - 308 3.1e-17 WDHD1 first WD40 domain
WD40_Prp19 PF24814 161 - 315 4.3e-13 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 164 - 311 1.7e-16 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 188 - 315 7.8e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 188 - 295 6.9e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 189 - 315 9.7e-14 THOC3 beta-propeller domain
EIF3I PF24805 189 - 320 3.1e-09 EIF3I
WD40_CDC20-Fz PF24807 189 - 312 4.7e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 190 - 305 6.4e-06 WDR90/POC16, second beta-propeller
WDR55 PF24796 212 - 322 1.4e-07 WDR55
Beta-prop_WDR3_1st PF25173 221 - 321 2.2e-09 WDR3 first beta-propeller domain
WD40 PF00400 262 - 295 8.5e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 449
Acc36I ACCTGC 1 cut(s) 199
AccBSI CCGCTC 1 cut(s) 250
AccI GTMKAC 2 cut(s) 331, 1034
AciI CCGC 8 cut(s) 79, 101, 165, 206, 248, 285, 1050, 1201
AclWI GGATC 4 cut(s) 594, 668, 681, 862
AcsI RAATTY 1 cut(s) 976
AcuI CTGAAG 1 cut(s) 1136
AcyI GRCGYC 1 cut(s) 867
AfaI GTAC 3 cut(s) 188, 494, 1112
AfiI CCNNNNNNNGG 2 cut(s) 107, 996
AflII CTTAAG 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 593
AgsI TTSAA 1 cut(s) 916
AjnI CCWGG 1 cut(s) 676
Alw21I GWGCWC 1 cut(s) 516
Alw26I GTCTC 4 cut(s) 447, 1047, 1186, 1309
AlwI GGATC 4 cut(s) 594, 668, 681, 862
Ama87I CYCGRG 2 cut(s) 68, 991
AoxI GGCC 4 cut(s) 15, 64, 109, 1075
ApeKI GCWGC 4 cut(s) 73, 254, 728, 1319
ApoI RAATTY 1 cut(s) 976
Asp700I GAANNNNTTC 1 cut(s) 224
AspLEI GCGC 1 cut(s) 95
AspS9I GGNCC 5 cut(s) 52, 65, 198, 929, 1076
AsuC2I CCSGG 1 cut(s) 1079
AsuHPI GGTGA 2 cut(s) 692, 1304
AvaI CYCGRG 2 cut(s) 68, 991
AvaII GGWCC 3 cut(s) 52, 198, 929
BamHI GGATCC 1 cut(s) 673
BanII GRGCYC 1 cut(s) 749
BbsI GAAGAC 1 cut(s) 682
Bbv12I GWGCWC 1 cut(s) 516
BbvCI CCTCAGC 1 cut(s) 1171
BbvI GCAGC 3 cut(s) 85, 241, 715
BccI CCATC 6 cut(s) 386, 416, 495, 704, 857, 1283
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 1 cut(s) 678
BclI TGATCA 1 cut(s) 1294
BcnI CCSGG 1 cut(s) 1079
BcoDI GTCTC 4 cut(s) 447, 1047, 1186, 1309
BfaI CTAG 3 cut(s) 818, 888, 947
BfmI CTRYAG 1 cut(s) 358
BfrI CTTAAG 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 199
BisI GCNGC 5 cut(s) 74, 206, 255, 729, 1320
BlpI GCTNAGC 1 cut(s) 1230
BlsI GCNGC 5 cut(s) 75, 207, 256, 730, 1321
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 2 cut(s) 678, 1079
Bme18I GGWCC 3 cut(s) 52, 198, 929
BmeT110I CYCGRG 2 cut(s) 68, 991
BmgT120I GGNCC 5 cut(s) 52, 65, 198, 929, 1076
BmiI GGNNCC 5 cut(s) 53, 67, 271, 397, 675
BmrFI CCNGG 2 cut(s) 678, 1079
BmsI GCATC 6 cut(s) 48, 545, 587, 649, 874, 1270
BpiI GAAGAC 1 cut(s) 682
Bpu10I CCTNAGC 1 cut(s) 1171
Bpu1102I GCTNAGC 1 cut(s) 1230
BpuEI CTTGAG 1 cut(s) 1181
BpuMI CCSGG 1 cut(s) 1079
Bsa29I ATCGAT 1 cut(s) 780
BsaBI GATNNNNATC 2 cut(s) 465, 1293
BsaHI GRCGYC 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 1069
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 996
Bse118I RCCGGY 2 cut(s) 35, 698
Bse1I ACTGG 7 cut(s) 184, 194, 331, 675, 838, 932, 1029
Bse3DI GCAATG 1 cut(s) 276
Bse8I GATNNNNATC 2 cut(s) 465, 1293
BseBI CCWGG 1 cut(s) 678
BseCI ATCGAT 1 cut(s) 780
BseDI CCNNGG 1 cut(s) 1069
BseGI GGATG 6 cut(s) 394, 427, 538, 560, 889, 1294
BseJI GATNNNNATC 2 cut(s) 465, 1293
BseLI CCNNNNNNNGG 2 cut(s) 107, 996
BseMI GCAATG 1 cut(s) 276
BseMII CTCAG 5 cut(s) 161, 732, 983, 1185, 1244
BseNI ACTGG 7 cut(s) 184, 194, 331, 675, 838, 932, 1029
BseRI GAGGAG 3 cut(s) 47, 133, 160
BseXI GCAGC 3 cut(s) 85, 241, 715
BsgI GTGCAG 1 cut(s) 714
BshFI GGCC 4 cut(s) 17, 66, 111, 1077
BshVI ATCGAT 1 cut(s) 780
BsiHKAI GWGCWC 1 cut(s) 516
BsiHKCI CYCGRG 2 cut(s) 68, 991
BsiSI CCGG 3 cut(s) 36, 699, 1079
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 2 cut(s) 107, 996
BsmAI GTCTC 4 cut(s) 447, 1047, 1186, 1309
BsmFI GGGAC 1 cut(s) 65
BsnI GGCC 4 cut(s) 17, 66, 111, 1077
Bso31I GGTCTC 1 cut(s) 447
BsoBI CYCGRG 2 cut(s) 68, 991
Bsp1286I GDGCHC 2 cut(s) 516, 749
Bsp1407I TGTACA 2 cut(s) 492, 1110
Bsp143I GATC 5 cut(s) 240, 586, 673, 854, 1294
Bsp1720I GCTNAGC 1 cut(s) 1230
BspACI CCGC 8 cut(s) 79, 101, 165, 206, 248, 285, 1050, 1201
BspANI GGCC 4 cut(s) 17, 66, 111, 1077
BspCNI CTCAG 5 cut(s) 160, 733, 982, 1184, 1243
BspDI ATCGAT 1 cut(s) 780
BspLI GGNNCC 5 cut(s) 53, 67, 271, 397, 675
BspMI ACCTGC 1 cut(s) 199
BspPI GGATC 4 cut(s) 594, 668, 681, 862
BspQI GCTCTTC 1 cut(s) 215
BspTI CTTAAG 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 447
BsrBI CCGCTC 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 276
BsrFI RCCGGY 2 cut(s) 35, 698
BsrGI TGTACA 2 cut(s) 492, 1110
BsrI ACTGG 7 cut(s) 184, 194, 331, 675, 838, 932, 1029
BssAI RCCGGY 2 cut(s) 35, 698
BssECI CCNNGG 1 cut(s) 1069
BssMI GATC 5 cut(s) 240, 586, 673, 854, 1294
BssNAI GTATAC 1 cut(s) 1035
BssNI GRCGYC 1 cut(s) 867
BssT1I CCWWGG 1 cut(s) 1069
Bst1107I GTATAC 1 cut(s) 1035
Bst2UI CCWGG 1 cut(s) 678
Bst6I CTCTTC 2 cut(s) 25, 215
BstACI GRCGYC 1 cut(s) 867
BstAFI CTTAAG 1 cut(s) 338
BstAPI GCANNNNNTGC 1 cut(s) 737
BstAUI TGTACA 2 cut(s) 492, 1110
BstC8I GCNNGC 4 cut(s) 37, 252, 283, 351
BstDEI CTNAG 5 cut(s) 147, 741, 969, 1171, 1230
BstENI CCTNNNNNAGG 1 cut(s) 994
BstF5I GGATG 6 cut(s) 394, 427, 538, 560, 889, 1294
BstHHI GCGC 1 cut(s) 95
BstKTI GATC 5 cut(s) 243, 589, 676, 857, 1297
BstMAI GTCTC 4 cut(s) 447, 1047, 1186, 1309
BstMBI GATC 5 cut(s) 240, 586, 673, 854, 1294
BstMWI GCNNNNNNNGC 3 cut(s) 117, 157, 737
BstNI CCWGG 1 cut(s) 678
BstNSI RCATGY 3 cut(s) 353, 597, 923
BstSCI CCNGG 2 cut(s) 676, 1077
BstSFI CTRYAG 1 cut(s) 358
BstV1I GCAGC 3 cut(s) 85, 241, 715
BstV2I GAAGAC 1 cut(s) 682
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 1 cut(s) 1225
BstYI RGATCY 1 cut(s) 673
BstZ17I GTATAC 1 cut(s) 1035
Bsu15I ATCGAT 1 cut(s) 780
BsuRI GGCC 4 cut(s) 17, 66, 111, 1077
BsuTUI ATCGAT 1 cut(s) 780
BtsCI GGATG 6 cut(s) 394, 427, 538, 560, 889, 1294
BtsI GCAGTG 1 cut(s) 1241
BtsIMutI CAGTG 5 cut(s) 668, 939, 1022, 1116, 1241
BveI ACCTGC 1 cut(s) 199
Cac8I GCNNGC 4 cut(s) 37, 252, 283, 351
CfoI GCGC 1 cut(s) 95
Cfr10I RCCGGY 2 cut(s) 35, 698
Cfr13I GGNCC 5 cut(s) 52, 65, 198, 929, 1076
ClaI ATCGAT 1 cut(s) 780
CseI GACGC 3 cut(s) 109, 856, 975
Csp6I GTAC 3 cut(s) 187, 493, 1111
CviAII CATG 5 cut(s) 107, 350, 594, 920, 1001
CviQI GTAC 3 cut(s) 187, 493, 1111
DdeI CTNAG 5 cut(s) 147, 741, 969, 1171, 1230
DpnI GATC 5 cut(s) 242, 588, 675, 856, 1296
DpnII GATC 5 cut(s) 240, 586, 673, 854, 1294
Eam1104I CTCTTC 2 cut(s) 25, 215
EarI CTCTTC 2 cut(s) 25, 215
EciI GGCGGA 3 cut(s) 90, 154, 1065
Eco130I CCWWGG 1 cut(s) 1069
Eco24I GRGCYC 1 cut(s) 749
Eco31I GGTCTC 1 cut(s) 447
Eco47I GGWCC 3 cut(s) 52, 198, 929
Eco57I CTGAAG 1 cut(s) 1136
Eco88I CYCGRG 2 cut(s) 68, 991
EcoNI CCTNNNNNAGG 1 cut(s) 994
EcoO109I RGGNCCY 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 976
EcoRII CCWGG 1 cut(s) 676
EcoT14I CCWWGG 1 cut(s) 1069
EcoT38I GRGCYC 1 cut(s) 749
ErhI CCWWGG 1 cut(s) 1069
FaeI CATG 5 cut(s) 110, 353, 597, 923, 1004
FaqI GGGAC 1 cut(s) 65
FatI CATG 5 cut(s) 106, 349, 593, 919, 1000
FauI CCCGC 1 cut(s) 1194
FauNDI CATATG 2 cut(s) 823, 982
FbaI TGATCA 1 cut(s) 1294
FblI GTMKAC 2 cut(s) 331, 1034
Fnu4HI GCNGC 5 cut(s) 74, 206, 255, 729, 1320
FokI GGATG 6 cut(s) 401, 434, 545, 567, 896, 1301
FriOI GRGCYC 1 cut(s) 749
Fsp4HI GCNGC 5 cut(s) 74, 206, 255, 729, 1320
FspBI CTAG 3 cut(s) 818, 888, 947
GlaI GCGC 1 cut(s) 94
GluI GCNGC 5 cut(s) 74, 206, 255, 729, 1320
HaeIII GGCC 4 cut(s) 17, 66, 111, 1077
HapII CCGG 3 cut(s) 36, 699, 1079
HgaI GACGC 3 cut(s) 109, 856, 975
HhaI GCGC 1 cut(s) 95
Hin1I GRCGYC 1 cut(s) 867
Hin1II CATG 5 cut(s) 110, 353, 597, 923, 1004
Hin6I GCGC 1 cut(s) 93
HinP1I GCGC 1 cut(s) 93
HincII GTYRAC 1 cut(s) 476
HindII GTYRAC 1 cut(s) 476
HinfI GANTC 3 cut(s) 132, 793, 1194
HpaII CCGG 3 cut(s) 36, 699, 1079
HphI GGTGA 2 cut(s) 692, 1304
Hpy166II GTNNAC 3 cut(s) 332, 476, 1035
Hpy188I TCNGA 4 cut(s) 388, 418, 859, 972
Hpy188III TCNNGA 3 cut(s) 790, 852, 993
Hpy8I GTNNAC 3 cut(s) 332, 476, 1035
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 4 cut(s) 171, 260, 990, 1040
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 9 cut(s) 558, 662, 668, 731, 772, 923, 1089, 1261, 1319
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 157, 737
HpyF3I CTNAG 5 cut(s) 147, 741, 969, 1171, 1230
HpySE526I ACGT 1 cut(s) 723
Hsp92I GRCGYC 1 cut(s) 867
Hsp92II CATG 5 cut(s) 110, 353, 597, 923, 1004
HspAI GCGC 1 cut(s) 93
KroI GCCGGC 1 cut(s) 35
KroNI GCCGGC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 1294
Kzo9I GATC 5 cut(s) 240, 586, 673, 854, 1294
LguI GCTCTTC 1 cut(s) 215
LmnI GCTCC 5 cut(s) 4, 81, 156, 401, 511
Lsp1109I GCAGC 3 cut(s) 85, 241, 715
LweI GCATC 6 cut(s) 48, 545, 587, 649, 874, 1270
MaeI CTAG 3 cut(s) 818, 888, 947
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 3 cut(s) 526, 538, 808
MalI GATC 5 cut(s) 242, 588, 675, 856, 1296
MbiI CCGCTC 1 cut(s) 250
MboI GATC 5 cut(s) 240, 586, 673, 854, 1294
MboII GAAGA 5 cut(s) 12, 232, 245, 682, 872
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 2 cut(s) 516, 749
MluCI AATT 6 cut(s) 502, 654, 663, 838, 976, 1093
MlyI GAGTC 1 cut(s) 1188
MroNI GCCGGC 1 cut(s) 35
MroXI GAANNNNTTC 1 cut(s) 224
MseI TTAA 4 cut(s) 321, 339, 735, 897
MslI CAYNNNNRTG 2 cut(s) 569, 1223
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 3 cut(s) 36, 699, 1079
MspR9I CCNGG 2 cut(s) 678, 1079
MunI CAATTG 1 cut(s) 663
MvaI CCWGG 1 cut(s) 678
MwoI GCNNNNNNNGC 3 cut(s) 117, 157, 737
NaeI GCCGGC 1 cut(s) 37
NciI CCSGG 1 cut(s) 1079
NdeI CATATG 2 cut(s) 823, 982
NdeII GATC 5 cut(s) 240, 586, 673, 854, 1294
NgoMIV GCCGGC 1 cut(s) 35
NlaIII CATG 5 cut(s) 110, 353, 597, 923, 1004
NlaIV GGNNCC 5 cut(s) 53, 67, 271, 397, 675
NmuCI GTSAC 1 cut(s) 538
NspI RCATGY 3 cut(s) 353, 597, 923
PaeI GCATGC 1 cut(s) 353
PaeR7I CTCGAG 1 cut(s) 991
PciI ACATGT 1 cut(s) 593
PciSI GCTCTTC 1 cut(s) 215
PdiI GCCGGC 1 cut(s) 37
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 2 cut(s) 132, 793
PkrI GCNGC 5 cut(s) 75, 207, 256, 730, 1321
PleI GAGTC 1 cut(s) 1188
PpsI GAGTC 1 cut(s) 1188
PpuMI RGGWCCY 1 cut(s) 52
PscI ACATGT 1 cut(s) 593
PsiI TTATAA 1 cut(s) 449
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 676
PspGI CCWGG 1 cut(s) 676
PspN4I GGNNCC 5 cut(s) 53, 67, 271, 397, 675
PspPI GGNCC 5 cut(s) 52, 65, 198, 929, 1076
PspPPI RGGWCCY 1 cut(s) 52
PsuI RGATCY 1 cut(s) 673
RsaI GTAC 3 cut(s) 188, 494, 1112
RsaNI GTAC 3 cut(s) 187, 493, 1111
RseI CAYNNNNRTG 2 cut(s) 569, 1223
SapI GCTCTTC 1 cut(s) 215
SaqAI TTAA 4 cut(s) 321, 339, 735, 897
SatI GCNGC 5 cut(s) 74, 206, 255, 729, 1320
Sau3AI GATC 5 cut(s) 240, 586, 673, 854, 1294
Sau96I GGNCC 5 cut(s) 52, 65, 198, 929, 1076
ScaI AGTACT 1 cut(s) 188
SchI GAGTC 1 cut(s) 1188
ScrFI CCNGG 2 cut(s) 678, 1079
SduI GDGCHC 2 cut(s) 516, 749
SfaNI GCATC 6 cut(s) 48, 545, 587, 649, 874, 1270
SfcI CTRYAG 1 cut(s) 358
Sfr274I CTCGAG 1 cut(s) 991
SinI GGWCC 3 cut(s) 52, 198, 929
SlaI CTCGAG 1 cut(s) 991
SmiMI CAYNNNNRTG 2 cut(s) 569, 1223
SmlI CTYRAG 3 cut(s) 338, 991, 1196
SmoI CTYRAG 3 cut(s) 338, 991, 1196
SphI GCATGC 1 cut(s) 353
Sse9I AATT 6 cut(s) 502, 654, 663, 838, 976, 1093
SsiI CCGC 8 cut(s) 79, 101, 165, 206, 248, 285, 1050, 1201
SspI AATATT 2 cut(s) 637, 943
SspMI CTAG 3 cut(s) 818, 888, 947
StyD4I CCNGG 2 cut(s) 676, 1077
StyI CCWWGG 1 cut(s) 1069
TaiI ACGT 1 cut(s) 726
TaqI TCGA 7 cut(s) 30, 459, 780, 791, 874, 955, 992
TasI AATT 6 cut(s) 502, 654, 663, 838, 976, 1093
TatI WGTACW 3 cut(s) 186, 492, 1110
TauI GCSGC 1 cut(s) 208
TfiI GAWTC 2 cut(s) 132, 793
Tru1I TTAA 4 cut(s) 321, 339, 735, 897
Tru9I TTAA 4 cut(s) 321, 339, 735, 897
TscAI CASTG 5 cut(s) 675, 939, 1029, 1123, 1241
TseFI GTSAC 1 cut(s) 538
TseI GCWGC 4 cut(s) 73, 254, 728, 1319
Tsp45I GTSAC 1 cut(s) 538
TspDTI ATGAA 5 cut(s) 969, 989, 996, 1145, 1224
TspGWI ACGGA 3 cut(s) 143, 524, 545
TspRI CASTG 5 cut(s) 675, 939, 1029, 1123, 1241
Vha464I CTTAAG 1 cut(s) 338
VpaK11BI GGWCC 3 cut(s) 52, 198, 929
XagI CCTNNNNNAGG 1 cut(s) 994
XapI RAATTY 1 cut(s) 976
XceI RCATGY 3 cut(s) 353, 597, 923
XhoI CTCGAG 1 cut(s) 991
XmiI GTMKAC 2 cut(s) 331, 1034
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 3 cut(s) 818, 888, 947
ZrmI AGTACT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.