FvH4_2g34950

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012367
Physical Location & Seq
Reverse (-)
25867758 .. 25872978
5221 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g34950.t1

Sequence Viewer

Length: 2001 bp
ATGGCGTCATCTTCTCCGCCGGAATCCAAGAAAATCGCAGCTCCTTTCGGCTCCTGGAAGTCCCCGATCACCGCCGATGTTGTCTCCGGCGCCTCCAAGCGTCTCGGAGGCACCGCCGTCGATTCCCGCGGCCGCCTCATCTGGCTAGAGTCTCGTCCGTCGGAATCCGGGCGAGCGGTTCTGGTGAGGGAACCGGAGAGACCGGAAGACGAACCGGTCGATATTACGCCGAAGGATTATGCGGTTCGATCTCTGGCGCAGGAGTACGGCGGTGGCGCCTTCCGTGTTTCAGGAGATACAGTTATCTTCTCTAACTATAAGGATCAACGCCTTTACAAGCAGTCTTTAAGTTCCAAAGATTCTTCACCAGTGCCGCTCACTCCGGACTATGGTGGACCGGTTGTTAGTTATGCTGATGGGGTATTTGATGCACGTTTTAATCGTTTTATCACCGTCAGGGAAGATTGTCGCGAAAGCAGCATCAATTCGATTACAACAATTGTCACGGTACGGCTAGATGGAAATGGCATTCAGGAATCAGAAACACTCGTAGGCGGAAGTGACTTCTATGCTTTCCCGCGTTTGGATCCCAAAGGAGAACGATTCGCATGGATTGAATGGTGCCATCCTAACATGCCATGGGATAGGTCTCAACTTTGGGTTGGGTATATATCTGAGAAAGGAGACGTCACGAAACGAGTATGTGTTTCTGGCTCTGATCCTGCAATCAAGGAGTCTCCAATTGAACCCAAGTGGTCCTCAAAAGGGGAACTATTTTTCATTACTGACCGTAAAAGTGGGTTTTGGAATCTCTATAAATGGGTTGAGTCTACTAATGAAGTCATATCAGTTTATTCTCTGGATGCTGAATTCTCCAAACCACTATGGGTTTTTGGCTTCAATTCTTATGAATTTATTGAGAGCCATGAACAAAAGAACCTAATTGCTTGCAGCTATAGGCAAAATGGGAAATCATATATTGGAATTGTGGATGATGTTCAGGGATCATTATCTCTTCTTAATATTCCTTTCACGGATATTGATAATATTACTTTGGGGATCAATTGCTTATATGTGGAAGGAGCATCAGAAGTTCATCCGTTGTCTGTTGCTAAGGTGACATTAGATGATCAAAAATCAAAAGCAGTTGAATTCAAGATTATTTGGTCTTCTTCACCTGATTGCTTAAAATATGAGTCCTACTTCAGCCTACCTGAATTGATTGAGTTCCCAACAGAAGTTCCTGGTCAAACTGCCTTTGCATACTTTTATCCTCCAACAAATCCTAGCTACCAAGGCACTCAGGACGAAAAACCTCCACTATTACTGAAAAGCCATGGAGGTCCCACGGCTGAAGCACGTGGAGTTCTAAATCTGAGTATTCAGTACTGGACTAGTCGTGGTTGGGCATTTGTTGATGTTAACTATGGTGGAAGCACAGGTTATGGCAGAGAATATCGTGAAAGGCTGTTGAAGAAATGGGGAATTGTGGATGTAAATGATTGTTGCAGCTGTGCTAGTTATTTGGTGGAATCTGGAAAGGTAGATGGGAAACGGCTTTGTATTACTGGGGGTTCTGCAGGTGGGTACACAACCTTAGCTGCTCTTGCATTTCGAGACACCTTCAAGGCTGGAGCTTCCTTGTATGGTGTAGCTGACTTAAACATGTTGAGGGCAGAAACTCACAAATTTGAATCTCGTTATCTGGATAATCTTGTTGGTAGTGACCAGGATTACTTTGATAGGTCACCAATCAACTTTGTTGATAAATTTTCCTGCCCAATAATCTTATTCCAAGGATTGGAAGACAAGGTTGTACCCCCAGATCAAGCTCGTACAATTTACCGTGCTCTGAAGGAAAAGGGCTTGCCAGTTGCCCTGGTGGAGTATGAAGGTGAACAACATGGTTTCCGCAAGCAAATGGTTTTCTTTGCACGTTTAGTTGGACACTTTAAGGTTGCAGATGATATTAGTCCAATCAAGATTGATAACTTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

667

Amino Acids

74.25

Weight (kDa)

5.28

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 459 - 640 1.2e-44 Prolyl oligopeptidase family
BD-FAE PF20434 498 - 618 9.3e-09 BD-FAE
DLH PF01738 507 - 639 1.2e-08 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36210
fragaria_vesca FvH4_2g34950
malus_domestica MD08G1107000.v1.1 MD08G1107200.v1.1 MD15G1087700.v1.1 MD15G1087800.v1.1
prunus_persica Prupe.1G441500_v2.0.a1
pyrus_communis pycom08g08900 pycom08g08910 pycom15g08240
rosa_chinensis RchiOBHm_Chr6g0307051
rosa_laevigata RLG00000010738
rosa_multiflora Rmu_sc0005634.1_g000006
rosa_roxburghii Rroxscaffold_7G00161200
rosa_rugosa Rorug06G0355500
rosa_samantha Rh6AG467000 Rh6CG482500 Rh6DG468800
rosa_wichuraiana Rw6G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1571
AasI GACNNNNNNGTC 1 cut(s) 215
AatII GACGTC 1 cut(s) 690
Acc36I ACCTGC 1 cut(s) 1571
AccB1I GGYRCC 4 cut(s) 89, 110, 275, 621
AccB7I CCANNNNNTGG 1 cut(s) 1801
AccBSI CCGCTC 2 cut(s) 176, 376
AccI GTMKAC 1 cut(s) 830
AccII CGCG 3 cut(s) 129, 471, 580
AccIII TCCGGA 1 cut(s) 382
AclWI GGATC 6 cut(s) 330, 581, 594, 713, 1012, 1067
AcoI YGGCCR 1 cut(s) 130
AcsI RAATTY 5 cut(s) 869, 911, 1151, 1688, 1769
AcuI CTGAAG 3 cut(s) 1189, 1374, 1874
AcvI CACGTG 1 cut(s) 1361
AcyI GRCGYC 4 cut(s) 5, 90, 276, 687
AfaI GTAC 6 cut(s) 266, 510, 1388, 1589, 1818, 1837
AfiI CCNNNNNNNGG 4 cut(s) 389, 583, 765, 1801
AflIII ACRYGT 1 cut(s) 1665
AgeI ACCGGT 2 cut(s) 214, 397
AgsI TTSAA 8 cut(s) 617, 746, 901, 1151, 1156, 1474, 1627, 1694
AhlI ACTAGT 1 cut(s) 1394
AjnI CCWGG 4 cut(s) 53, 1243, 1728, 1878
AluBI AGCT 8 cut(s) 41, 954, 1290, 1512, 1601, 1637, 1655, 1832
AluI AGCT 8 cut(s) 41, 954, 1290, 1512, 1601, 1637, 1655, 1832
Alw21I GWGCWC 1 cut(s) 1852
Alw26I GTCTC 8 cut(s) 88, 107, 156, 193, 654, 678, 741, 1611
AlwI GGATC 6 cut(s) 330, 581, 594, 713, 1012, 1067
Aor13HI TCCGGA 1 cut(s) 382
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 5 cut(s) 38, 477, 951, 1509, 1601
ApoI RAATTY 5 cut(s) 869, 911, 1151, 1688, 1769
AsiGI ACCGGT 2 cut(s) 214, 397
AspLEI GCGC 3 cut(s) 92, 259, 278
AspS9I GGNCC 3 cut(s) 395, 756, 1343
AsuC2I CCSGG 1 cut(s) 169
AsuHPI GGTGA 8 cut(s) 61, 196, 357, 442, 1129, 1167, 1740, 1907
AvaII GGWCC 3 cut(s) 395, 756, 1343
BamHI GGATCC 1 cut(s) 586
BanI GGYRCC 4 cut(s) 89, 110, 275, 621
BbrPI CACGTG 1 cut(s) 1361
BbsI GAAGAC 3 cut(s) 213, 1161, 1812
Bbv12I GWGCWC 1 cut(s) 1852
BbvI GCAGC 5 cut(s) 50, 489, 963, 1521, 1588
BccI CCATC 4 cut(s) 410, 512, 633, 1541
BceAI ACGGC 5 cut(s) 101, 283, 527, 1365, 1571
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 4 cut(s) 55, 1245, 1730, 1880
BclI TGATCA 1 cut(s) 1129
BcnI CCSGG 1 cut(s) 169
BcoDI GTCTC 8 cut(s) 88, 107, 156, 193, 654, 678, 741, 1611
BcuI ACTAGT 1 cut(s) 1394
BfaI CTAG 5 cut(s) 146, 515, 1287, 1395, 1518
BfmI CTRYAG 2 cut(s) 955, 1578
BfoI RGCGCY 2 cut(s) 93, 279
BfuAI ACCTGC 1 cut(s) 1571
BisI GCNGC 8 cut(s) 39, 130, 133, 374, 478, 952, 1510, 1602
BlsI GCNGC 8 cut(s) 40, 131, 134, 375, 479, 953, 1511, 1603
BmcAI AGTACT 1 cut(s) 1388
Bme1390I CCNGG 5 cut(s) 55, 169, 1245, 1730, 1880
Bme18I GGWCC 3 cut(s) 395, 756, 1343
BmgT120I GGNCC 3 cut(s) 395, 756, 1343
BmiI GGNNCC 8 cut(s) 52, 91, 112, 192, 277, 588, 623, 1345
BmrFI CCNGG 5 cut(s) 55, 169, 1245, 1730, 1880
BmrI ACTGGG 1 cut(s) 1578
BmsI GCATC 4 cut(s) 418, 489, 853, 1094
BmuI ACTGGG 1 cut(s) 1578
BpiI GAAGAC 3 cut(s) 213, 1161, 1812
BpmI CTGGAG 1 cut(s) 1653
Bpu10I CCTNAGC 2 cut(s) 1113, 1597
BpuMI CCSGG 1 cut(s) 169
BsaAI YACGTR 1 cut(s) 1361
BsaBI GATNNNNATC 1 cut(s) 1009
BsaHI GRCGYC 4 cut(s) 5, 90, 276, 687
BsaI GGTCTC 2 cut(s) 193, 654
BsaJI CCNNGG 7 cut(s) 127, 638, 1294, 1336, 1347, 1795, 1878
BsaWI WCCGGW 5 cut(s) 193, 202, 214, 382, 397
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 4 cut(s) 389, 583, 765, 1801
Bse118I RCCGGY 2 cut(s) 214, 397
Bse1I ACTGG 4 cut(s) 368, 1394, 1573, 1871
Bse8I GATNNNNATC 1 cut(s) 1009
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 4 cut(s) 55, 1245, 1730, 1880
BseDI CCNNGG 7 cut(s) 127, 638, 1294, 1336, 1347, 1795, 1878
BseGI GGATG 5 cut(s) 625, 868, 997, 1096, 1498
BseJI GATNNNNATC 1 cut(s) 1009
BseLI CCNNNNNNNGG 4 cut(s) 389, 583, 765, 1801
BseMII CTCAG 3 cut(s) 666, 1316, 1367
BseNI ACTGG 4 cut(s) 368, 1394, 1573, 1871
BseX3I CGGCCG 1 cut(s) 130
BseXI GCAGC 5 cut(s) 50, 489, 963, 1521, 1588
Bsh1236I CGCG 3 cut(s) 129, 471, 580
Bsh1285I CGRYCG 2 cut(s) 133, 219
BshFI GGCC 1 cut(s) 132
BshNI GGYRCC 4 cut(s) 89, 110, 275, 621
BshTI ACCGGT 2 cut(s) 214, 397
BsiEI CGRYCG 2 cut(s) 133, 219
BsiHKAI GWGCWC 1 cut(s) 1852
BsiSI CCGG 8 cut(s) 20, 87, 168, 194, 203, 215, 383, 398
BslFI GGGAC 2 cut(s) 46, 1329
BslI CCNNNNNNNGG 4 cut(s) 389, 583, 765, 1801
BsmAI GTCTC 8 cut(s) 88, 107, 156, 193, 654, 678, 741, 1611
BsmBI CGTCTC 2 cut(s) 107, 678
BsmFI GGGAC 2 cut(s) 46, 1329
BsmI GAATGC 1 cut(s) 528
BsnI GGCC 1 cut(s) 132
Bso31I GGTCTC 2 cut(s) 193, 654
Bsp1286I GDGCHC 1 cut(s) 1852
Bsp13I TCCGGA 1 cut(s) 382
Bsp143I GATC 9 cut(s) 66, 248, 322, 586, 718, 1004, 1059, 1129, 1825
Bsp19I CCATGG 2 cut(s) 638, 1336
Bsp68I TCGCGA 1 cut(s) 471
BspANI GGCC 1 cut(s) 132
BspCNI CTCAG 3 cut(s) 667, 1315, 1368
BspEI TCCGGA 1 cut(s) 382
BspFNI CGCG 3 cut(s) 129, 471, 580
BspLI GGNNCC 8 cut(s) 52, 91, 112, 192, 277, 588, 623, 1345
BspMAI CTGCAG 1 cut(s) 1582
BspMI ACCTGC 1 cut(s) 1571
BspPI GGATC 6 cut(s) 330, 581, 594, 713, 1012, 1067
BspT107I GGYRCC 4 cut(s) 89, 110, 275, 621
BspTNI GGTCTC 2 cut(s) 193, 654
BsrBI CCGCTC 2 cut(s) 176, 376
BsrFI RCCGGY 2 cut(s) 214, 397
BsrI ACTGG 4 cut(s) 368, 1394, 1573, 1871
BssAI RCCGGY 2 cut(s) 214, 397
BssECI CCNNGG 7 cut(s) 127, 638, 1294, 1336, 1347, 1795, 1878
BssMI GATC 9 cut(s) 66, 248, 322, 586, 718, 1004, 1059, 1129, 1825
BssNI GRCGYC 4 cut(s) 5, 90, 276, 687
BssT1I CCWWGG 4 cut(s) 638, 1294, 1336, 1795
Bst2UI CCWGG 4 cut(s) 55, 1245, 1730, 1880
Bst4CI ACNGT 5 cut(s) 301, 454, 508, 791, 1847
Bst6I CTCTTC 1 cut(s) 1020
BstACI GRCGYC 4 cut(s) 5, 90, 276, 687
BstBAI YACGTR 1 cut(s) 1361
BstC8I GCNNGC 4 cut(s) 174, 949, 1868, 1916
BstDEI CTNAG 5 cut(s) 675, 1113, 1302, 1376, 1597
BstDSI CCRYGG 4 cut(s) 127, 638, 1336, 1347
BstEII GGTNACC 1 cut(s) 1746
BstF5I GGATG 5 cut(s) 625, 868, 997, 1096, 1498
BstFNI CGCG 3 cut(s) 129, 471, 580
BstH2I RGCGCY 2 cut(s) 93, 279
BstHHI GCGC 3 cut(s) 92, 259, 278
BstKTI GATC 9 cut(s) 69, 251, 325, 589, 721, 1007, 1062, 1132, 1828
BstMAI GTCTC 8 cut(s) 88, 107, 156, 193, 654, 678, 741, 1611
BstMBI GATC 9 cut(s) 66, 248, 322, 586, 718, 1004, 1059, 1129, 1825
BstMCI CGRYCG 2 cut(s) 133, 219
BstMWI GCNNNNNNNGC 3 cut(s) 477, 1296, 1607
BstNI CCWGG 4 cut(s) 55, 1245, 1730, 1880
BstNSI RCATGY 2 cut(s) 637, 1669
BstPI GGTNACC 1 cut(s) 1746
BstSCI CCNGG 5 cut(s) 53, 167, 1243, 1728, 1878
BstSFI CTRYAG 2 cut(s) 955, 1578
BstUI CGCG 3 cut(s) 129, 471, 580
BstV1I GCAGC 5 cut(s) 50, 489, 963, 1521, 1588
BstV2I GAAGAC 3 cut(s) 213, 1161, 1812
BstX2I RGATCY 1 cut(s) 586
BstYI RGATCY 1 cut(s) 586
BstZI CGGCCG 1 cut(s) 130
BsuRI GGCC 1 cut(s) 132
BtgI CCRYGG 4 cut(s) 127, 638, 1336, 1347
BtsCI GGATG 5 cut(s) 625, 868, 997, 1096, 1498
BtsIMutI CAGTG 1 cut(s) 375
BtuMI TCGCGA 1 cut(s) 471
BveI ACCTGC 1 cut(s) 1571
Cac8I GCNNGC 4 cut(s) 174, 949, 1868, 1916
CciNI GCGGCCGC 1 cut(s) 130
CfoI GCGC 3 cut(s) 92, 259, 278
Cfr10I RCCGGY 2 cut(s) 214, 397
Cfr13I GGNCC 3 cut(s) 395, 756, 1343
Cfr42I CCGCGG 1 cut(s) 130
CseI GACGC 1 cut(s) 89
Csp6I GTAC 6 cut(s) 265, 509, 1387, 1588, 1817, 1836
CspAI ACCGGT 2 cut(s) 214, 397
CviAII CATG 7 cut(s) 609, 634, 639, 926, 1337, 1666, 1904
CviQI GTAC 6 cut(s) 265, 509, 1387, 1588, 1817, 1836
DdeI CTNAG 5 cut(s) 675, 1113, 1302, 1376, 1597
DinI GGCGCC 2 cut(s) 91, 277
DpnI GATC 9 cut(s) 68, 250, 324, 588, 720, 1006, 1061, 1131, 1827
DpnII GATC 9 cut(s) 66, 248, 322, 586, 718, 1004, 1059, 1129, 1825
DrdI GACNNNNNNGTC 1 cut(s) 215
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 1 cut(s) 130
EagI CGGCCG 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 1020
EarI CTCTTC 1 cut(s) 1020
EciI GGCGGA 2 cut(s) 6, 570
EclXI CGGCCG 1 cut(s) 130
Eco130I CCWWGG 4 cut(s) 638, 1294, 1336, 1795
Eco31I GGTCTC 2 cut(s) 193, 654
Eco47I GGWCC 3 cut(s) 395, 756, 1343
Eco52I CGGCCG 1 cut(s) 130
Eco57I CTGAAG 3 cut(s) 1189, 1374, 1874
Eco72I CACGTG 1 cut(s) 1361
Eco91I GGTNACC 1 cut(s) 1746
EcoO109I RGGNCCY 1 cut(s) 1343
EcoO65I GGTNACC 1 cut(s) 1746
EcoRI GAATTC 2 cut(s) 869, 1151
EcoRII CCWGG 4 cut(s) 53, 1243, 1728, 1878
EcoT14I CCWWGG 4 cut(s) 638, 1294, 1336, 1795
EgeI GGCGCC 2 cut(s) 91, 277
EheI GGCGCC 2 cut(s) 91, 277
ErhI CCWWGG 4 cut(s) 638, 1294, 1336, 1795
Esp3I CGTCTC 2 cut(s) 107, 678
FaeI CATG 7 cut(s) 612, 637, 642, 929, 1340, 1669, 1907
FaqI GGGAC 2 cut(s) 46, 1329
FatI CATG 7 cut(s) 608, 633, 638, 925, 1336, 1665, 1903
FauI CCCGC 2 cut(s) 134, 585
FbaI TGATCA 1 cut(s) 1129
FblI GTMKAC 1 cut(s) 830
Fnu4HI GCNGC 8 cut(s) 39, 130, 133, 374, 478, 952, 1510, 1602
FokI GGATG 5 cut(s) 612, 875, 1004, 1083, 1505
Fsp4HI GCNGC 8 cut(s) 39, 130, 133, 374, 478, 952, 1510, 1602
FspBI CTAG 5 cut(s) 146, 515, 1287, 1395, 1518
GlaI GCGC 3 cut(s) 91, 258, 277
GluI GCNGC 8 cut(s) 39, 130, 133, 374, 478, 952, 1510, 1602
GsuI CTGGAG 1 cut(s) 1653
HaeII RGCGCY 2 cut(s) 93, 279
HaeIII GGCC 1 cut(s) 132
HapII CCGG 8 cut(s) 20, 87, 168, 194, 203, 215, 383, 398
HgaI GACGC 1 cut(s) 89
HhaI GCGC 3 cut(s) 92, 259, 278
Hin1I GRCGYC 4 cut(s) 5, 90, 276, 687
Hin1II CATG 7 cut(s) 612, 637, 642, 929, 1340, 1669, 1907
Hin6I GCGC 3 cut(s) 90, 257, 276
HinP1I GCGC 3 cut(s) 90, 257, 276
HincII GTYRAC 1 cut(s) 1423
HindII GTYRAC 1 cut(s) 1423
HpaI GTTAAC 1 cut(s) 1423
HpaII CCGG 8 cut(s) 20, 87, 168, 194, 203, 215, 383, 398
HphI GGTGA 8 cut(s) 61, 196, 357, 442, 1129, 1167, 1740, 1907
Hpy166II GTNNAC 5 cut(s) 395, 831, 1423, 1590, 1898
Hpy188I TCNGA 8 cut(s) 107, 163, 541, 676, 718, 1090, 1377, 1854
Hpy8I GTNNAC 5 cut(s) 395, 831, 1423, 1590, 1898
Hpy99I CGWCG 2 cut(s) 122, 163
HpyAV CCTTC 6 cut(s) 226, 289, 1073, 1633, 1849, 1886
HpyCH4III ACNGT 5 cut(s) 301, 454, 508, 791, 1847
HpyCH4IV ACGT 4 cut(s) 433, 687, 1360, 1936
HpyCH4V TGCA 9 cut(s) 431, 725, 951, 1262, 1509, 1580, 1610, 1934, 1961
HpyF10VI GCNNNNNNNGC 3 cut(s) 477, 1296, 1607
HpyF3I CTNAG 5 cut(s) 675, 1113, 1302, 1376, 1597
HpySE526I ACGT 4 cut(s) 433, 687, 1360, 1936
Hsp92I GRCGYC 4 cut(s) 5, 90, 276, 687
Hsp92II CATG 7 cut(s) 612, 637, 642, 929, 1340, 1669, 1907
HspAI GCGC 3 cut(s) 90, 257, 276
KasI GGCGCC 2 cut(s) 89, 275
Kpn2I TCCGGA 1 cut(s) 382
Ksp22I TGATCA 1 cut(s) 1129
KspAI GTTAAC 1 cut(s) 1423
KspI CCGCGG 1 cut(s) 130
Kzo9I GATC 9 cut(s) 66, 248, 322, 586, 718, 1004, 1059, 1129, 1825
LmnI GCTCC 4 cut(s) 46, 56, 1082, 1634
Lsp1109I GCAGC 5 cut(s) 50, 489, 963, 1521, 1588
LweI GCATC 4 cut(s) 418, 489, 853, 1094
MaeI CTAG 5 cut(s) 146, 515, 1287, 1395, 1518
MaeII ACGT 4 cut(s) 433, 687, 1360, 1936
MaeIII GTNAC 6 cut(s) 502, 560, 688, 1117, 1724, 1746
MalI GATC 9 cut(s) 68, 250, 324, 588, 720, 1006, 1061, 1131, 1827
MbiI CCGCTC 2 cut(s) 176, 376
MboI GATC 9 cut(s) 66, 248, 322, 586, 718, 1004, 1059, 1129, 1825
MfeI CAATTG 3 cut(s) 498, 741, 1063
MflI RGATCY 1 cut(s) 586
MhlI GDGCHC 1 cut(s) 1852
Mly113I GGCGCC 2 cut(s) 90, 276
MlyI GAGTC 4 cut(s) 158, 743, 836, 1205
MmeI TCCRAC 3 cut(s) 141, 1301, 1924
MnlI CCTC 9 cut(s) 101, 103, 146, 180, 769, 1284, 1326, 1334, 1665
MroI TCCGGA 1 cut(s) 382
MseI TTAA 7 cut(s) 347, 438, 1020, 1187, 1422, 1661, 1953
MspA1I CMGCKG 2 cut(s) 129, 1512
MspI CCGG 8 cut(s) 20, 87, 168, 194, 203, 215, 383, 398
MspR9I CCNGG 5 cut(s) 55, 169, 1245, 1730, 1880
MunI CAATTG 3 cut(s) 498, 741, 1063
Mva1269I GAATGC 1 cut(s) 528
MvaI CCWGG 4 cut(s) 55, 1245, 1730, 1880
MvnI CGCG 3 cut(s) 129, 471, 580
MwoI GCNNNNNNNGC 3 cut(s) 477, 1296, 1607
NarI GGCGCC 2 cut(s) 90, 276
NciI CCSGG 1 cut(s) 169
NcoI CCATGG 2 cut(s) 638, 1336
NdeII GATC 9 cut(s) 66, 248, 322, 586, 718, 1004, 1059, 1129, 1825
NlaIII CATG 7 cut(s) 612, 637, 642, 929, 1340, 1669, 1907
NlaIV GGNNCC 8 cut(s) 52, 91, 112, 192, 277, 588, 623, 1345
NmuCI GTSAC 6 cut(s) 502, 560, 688, 1117, 1724, 1746
NotI GCGGCCGC 1 cut(s) 130
NruI TCGCGA 1 cut(s) 471
NspI RCATGY 2 cut(s) 637, 1669
PaqCI CACCTGC 1 cut(s) 1571
PciI ACATGT 1 cut(s) 1665
PcsI WCGNNNNNNNCGW 2 cut(s) 216, 439
PctI GAATGC 1 cut(s) 528
PfeI GAWTC 9 cut(s) 23, 122, 164, 359, 536, 603, 808, 1532, 1694
PflMI CCANNNNNTGG 1 cut(s) 1801
PfoI TCCNGGA 1 cut(s) 53
PinAI ACCGGT 2 cut(s) 214, 397
PkrI GCNGC 8 cut(s) 40, 131, 134, 375, 479, 953, 1511, 1603
PleI GAGTC 4 cut(s) 157, 742, 835, 1204
PluTI GGCGCC 2 cut(s) 93, 279
PmaCI CACGTG 1 cut(s) 1361
PmlI CACGTG 1 cut(s) 1361
PpsI GAGTC 4 cut(s) 157, 742, 835, 1204
Ppu21I YACGTR 1 cut(s) 1361
PpuMI RGGWCCY 1 cut(s) 1343
PscI ACATGT 1 cut(s) 1665
Psp5II RGGWCCY 1 cut(s) 1343
Psp6I CCWGG 4 cut(s) 53, 1243, 1728, 1878
PspCI CACGTG 1 cut(s) 1361
PspEI GGTNACC 1 cut(s) 1746
PspGI CCWGG 4 cut(s) 53, 1243, 1728, 1878
PspN4I GGNNCC 8 cut(s) 52, 91, 112, 192, 277, 588, 623, 1345
PspPI GGNCC 3 cut(s) 395, 756, 1343
PspPPI RGGWCCY 1 cut(s) 1343
PstI CTGCAG 1 cut(s) 1582
PsuI RGATCY 1 cut(s) 586
PvuII CAGCTG 1 cut(s) 1512
RruI TCGCGA 1 cut(s) 471
RsaI GTAC 6 cut(s) 266, 510, 1388, 1589, 1818, 1837
RsaNI GTAC 6 cut(s) 265, 509, 1387, 1588, 1817, 1836
SacII CCGCGG 1 cut(s) 130
SaqAI TTAA 7 cut(s) 347, 438, 1020, 1187, 1422, 1661, 1953
SatI GCNGC 8 cut(s) 39, 130, 133, 374, 478, 952, 1510, 1602
Sau3AI GATC 9 cut(s) 66, 248, 322, 586, 718, 1004, 1059, 1129, 1825
Sau96I GGNCC 3 cut(s) 395, 756, 1343
ScaI AGTACT 1 cut(s) 1388
SchI GAGTC 4 cut(s) 158, 743, 836, 1205
ScrFI CCNGG 5 cut(s) 55, 169, 1245, 1730, 1880
SduI GDGCHC 1 cut(s) 1852
SfaNI GCATC 4 cut(s) 418, 489, 853, 1094
SfcI CTRYAG 2 cut(s) 955, 1578
SfoI GGCGCC 2 cut(s) 91, 277
Sfr303I CCGCGG 1 cut(s) 130
SgrBI CCGCGG 1 cut(s) 130
SinI GGWCC 3 cut(s) 395, 756, 1343
SpeI ACTAGT 1 cut(s) 1394
SspDI GGCGCC 2 cut(s) 89, 275
SspI AATATT 2 cut(s) 1024, 1048
SspMI CTAG 5 cut(s) 146, 515, 1287, 1395, 1518
StyD4I CCNGG 5 cut(s) 53, 167, 1243, 1728, 1878
StyI CCWWGG 4 cut(s) 638, 1294, 1336, 1795
TaaI ACNGT 5 cut(s) 301, 454, 508, 791, 1847
TaiI ACGT 4 cut(s) 436, 690, 1363, 1939
TaqI TCGA 5 cut(s) 120, 219, 247, 488, 1615
TatI WGTACW 1 cut(s) 1386
TauI GCSGC 3 cut(s) 132, 135, 376
TfiI GAWTC 9 cut(s) 23, 122, 164, 359, 536, 603, 808, 1532, 1694
Tru1I TTAA 7 cut(s) 347, 438, 1020, 1187, 1422, 1661, 1953
Tru9I TTAA 7 cut(s) 347, 438, 1020, 1187, 1422, 1661, 1953
TscAI CASTG 1 cut(s) 375
TseFI GTSAC 6 cut(s) 502, 560, 688, 1117, 1724, 1746
TseI GCWGC 5 cut(s) 38, 477, 951, 1509, 1601
Tsp45I GTSAC 6 cut(s) 502, 560, 688, 1117, 1724, 1746
TspDTI ATGAA 6 cut(s) 769, 852, 924, 942, 1085, 1905
TspGWI ACGGA 4 cut(s) 147, 272, 1049, 1089
TspRI CASTG 1 cut(s) 375
Van91I CCANNNNNTGG 1 cut(s) 1801
VpaK11BI GGWCC 3 cut(s) 395, 756, 1343
XapI RAATTY 5 cut(s) 869, 911, 1151, 1688, 1769
XceI RCATGY 2 cut(s) 637, 1669
XmiI GTMKAC 1 cut(s) 830
XspI CTAG 5 cut(s) 146, 515, 1287, 1395, 1518
ZraI GACGTC 1 cut(s) 688
ZrmI AGTACT 1 cut(s) 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.