RchiOBHm_Chr6g0307051

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012367
Physical Location & Seq
Forward (+)
65578442 .. 65583896
5455 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27606

Sequence Viewer

Length: 2163 bp
ATGGCTCTCTCGTCCATCGCGACTCTCACTCGCTTCTCTACTACTACTTCTTCTTCTCACTTCCACTGCATCAGTAAACGGCTCAGACTCATCGCCAGAGCACCAACCAAAAGTCAGCACCGCCAGAAAATGACGTCATCTTCTCGGGAAGCCAAGCAAGAGAAAATCGCAGCTCCTTTCGGCTCGTGGAAGTCCCCAATCACCGCCGATGTCGTCTCCGGCGCCTCCAAGCGGCTCGGCGGCACCGCCGTCGATTCCCACGGCCGCCTCATCTGGCTCGAGTCTCGTCCGTCGGAATCCGGACGAGCGGTTCTGGTGAGAGAACCGGAGAGACCGGAAGACGAACCGGTTGATATCACGCCGAAGGACTACGCGGTTCGAACTCTGGCTCAGGAGTACGGCGGAGGCGCCTTCAGCGTTTCAGGAGATACACTTGTCTTCTCAAATTATAAGGATCAACGCCTTTACAAGCAGTCTTTAAGCTCCAAAGATTCTTCACCAGTACCACTCACTCCAGATTATGGTGGACCAGTTGTTAGTTATGCTGATGGGGTGTTTGATGCACGTTTTAACCGTTTTATCACTGTGAGGGAAGATTGTCGCGAAAGCAGCATGAATTCAATCACAACAATTGTAACAGTAAGGCTTGATGGAAAGGACATTCAGGAATCAGAAACACTCGTAGGTGGATGTGACTTCTATGCCTTCCCGCGTCTGGATCCCAAAGGAGAACGACTTGCATGGATTGAATGGTGCCATCCTAACATGCCATGGGATAGGTCTCAACTTTGGGTTGGGTATATATCTGAGAAAGGGGAAGTCTCAAAACGAGTCTGTGTTTCTGGCTCTGATCCTGCAATTAAGGAATCTCCAATTGAACCCAAGTGGTCCGCAAAAGGGGAACTATTTTTCATTACTGACAGAAAAGGTGGGTTTTGGAATCTCTATAAATGGATTGAGTCTACTAATGAGGTCGTATCAGTTTATTCTTTGGATGCTGAATTTTCCAAACCACTATGGGTTTTTGGCTTGAATTCTTATGAATTTATTGAGAGCCATGAACAAAAGAACCTTATTGCTTGCAGCTATAGGAAAAACGGGAGATCATATCTTGGAATTGTGGATGATATTCAGGGCTCATTATCCCTGCTTAATATTCCTTTCACGGATATTGATAATATTACTTTGGGGTTCAATTGCCTATATGTGGAAGGAGCATCAGAAGTTCATCCATTGTCTGTTGCTAAGGTGACATTAGATGATCAAAAATCAAAAGCAGTTGAATTCAAGATTATTTGGTCTTCTTCACCCGATTGCTTAAAATATGAGTCCTACTTCAGCCTACCTGAATTAATTGAGTTCCCAACAGAAGTTCCTGGTCAAACTGCCTTTGCATACTTTTATCCACCATCAAATCCTAGCTACCAAGCAACTAAGGATGAAAAGCCTCCACTATTACTGAAAAGCCATGGAGGACCCACAGCTGAAGCACGTGGAATTTTAAATCTAAGTGTTCAGTACTGGACTAGTCGTGGTTGGGCATTCGTTGATGTTAACTATGGTGGAAGCACTGGTTATGGCAGAGAATATCGTGAAAGGCTGTTGAAGAAATGGGGAATTGTGGATGTAAATGATTGTTGCAGCTGTGCTAGTTATCTGGTGGAATCTGGAAAGGTAGATGGGAAACGACTTTGTATTACGGGAGGCTCTGCAGGTGGATACACAACCTTAGCTGCACTTGCATTTCGAGACACCTTCAAGGCTGGAGCTTCCTTGTATGGTGTAGCTGACTTAAACATGTTGAGGGCTGAAACTCACAAATTTGAATCTCATTATATGGATAATCTTGTTGGTAGTGATCAGGATTTCTTTGATAGATCACCAATCAACTTTGTTGATAAATTTTCTTGCCCAATAATCTTATTCCAAGGATTGGAAGACAAGGTTGTACCCCCAGATCAAGCTCGTACAATTTACCGTGCTCTGAAGGAAAAGGGCTTGCCAGTTGCTCTCGTGGAGTATGAAGGAGAACAACATGGTTTCCGCAAGGCTGAGAACATTATATTCACTCTGGAACAGCAAATGGTTTTCTTTGCACGTTTAGTTGGACACTTCAAGGTTGCAGATGAGATTACTCCGATCAAAATTGATAACTTGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

720

Amino Acids

80.49

Weight (kDa)

5.88

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 504 - 703 2.7e-47 Prolyl oligopeptidase family
BD-FAE PF20434 542 - 662 8.4e-09 BD-FAE
DLH PF01738 551 - 687 5.3e-09 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36210
fragaria_vesca FvH4_2g34950
malus_domestica MD08G1107000.v1.1 MD08G1107200.v1.1 MD15G1087700.v1.1 MD15G1087800.v1.1
prunus_persica Prupe.1G441500_v2.0.a1
pyrus_communis pycom08g08900 pycom08g08910 pycom15g08240
rosa_chinensis RchiOBHm_Chr6g0307051
rosa_laevigata RLG00000010738
rosa_multiflora Rmu_sc0005634.1_g000006
rosa_roxburghii Rroxscaffold_7G00161200
rosa_rugosa Rorug06G0355500
rosa_samantha Rh6AG467000 Rh6CG482500 Rh6DG468800
rosa_wichuraiana Rw6G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AarI CACCTGC 1 cut(s) 1703
AatII GACGTC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 1703
AccB1I GGYRCC 4 cut(s) 221, 242, 407, 753
AccB7I CCANNNNNTGG 2 cut(s) 521, 1933
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 962
AccII CGCG 4 cut(s) 20, 374, 603, 712
AccIII TCCGGA 1 cut(s) 299
AclWI GGATC 4 cut(s) 462, 713, 726, 845
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 8 cut(s) 616, 1001, 1033, 1043, 1283, 1497, 1820, 1901
AcuI CTGAAG 4 cut(s) 397, 1321, 1506, 2006
AcvI CACGTG 1 cut(s) 1493
AcyI GRCGYC 3 cut(s) 134, 222, 408
AfaI GTAC 5 cut(s) 398, 504, 1520, 1950, 1969
AfiI CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1933
AflIII ACRYGT 1 cut(s) 1797
AgeI ACCGGT 1 cut(s) 346
AhlI ACTAGT 1 cut(s) 1526
AjnI CCWGG 1 cut(s) 1375
AloI GAACNNNNNNTCC 2 cut(s) 294, 326
Alw21I GWGCWC 2 cut(s) 103, 1984
Alw26I GTCTC 6 cut(s) 220, 288, 325, 786, 826, 1743
AlwI GGATC 4 cut(s) 462, 713, 726, 845
Ama87I CYCGRG 2 cut(s) 144, 278
Aor13HI TCCGGA 1 cut(s) 299
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 5 cut(s) 170, 609, 1083, 1641, 1733
ApoI RAATTY 8 cut(s) 616, 1001, 1033, 1043, 1283, 1497, 1820, 1901
AseI ATTAAT 1 cut(s) 1352
AsiGI ACCGGT 1 cut(s) 346
AspLEI GCGC 2 cut(s) 224, 410
AspS9I GGNCC 3 cut(s) 527, 888, 1475
AsuHPI GGTGA 6 cut(s) 193, 328, 489, 1261, 1299, 1872
AsuII TTCGAA 1 cut(s) 379
AvaI CYCGRG 2 cut(s) 144, 278
AvaII GGWCC 3 cut(s) 527, 888, 1475
BaeI ACNNNNGTAYC 2 cut(s) 420, 453
BamHI GGATCC 1 cut(s) 718
BanI GGYRCC 4 cut(s) 221, 242, 407, 753
BanII GRGCYC 1 cut(s) 1139
BauI CACGAG 2 cut(s) 184, 2012
BbrPI CACGTG 1 cut(s) 1493
BbsI GAAGAC 4 cut(s) 345, 430, 1293, 1944
Bbv12I GWGCWC 2 cut(s) 103, 1984
BbvI GCAGC 5 cut(s) 182, 621, 1095, 1653, 1720
BccI CCATC 6 cut(s) 23, 542, 644, 765, 1417, 1673
BceAI ACGGC 4 cut(s) 95, 233, 277, 415
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 1 cut(s) 1377
BciVI GTATCC 1 cut(s) 1712
BclI TGATCA 2 cut(s) 1261, 1858
BcoDI GTCTC 6 cut(s) 220, 288, 325, 786, 826, 1743
BcuI ACTAGT 1 cut(s) 1526
BfaI CTAG 3 cut(s) 1419, 1527, 1650
BfmI CTRYAG 2 cut(s) 1087, 1710
BfoI RGCGCY 2 cut(s) 225, 411
BfuAI ACCTGC 1 cut(s) 1703
BfuI GTATCC 1 cut(s) 1712
BisI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1734
BlsI GCNGC 8 cut(s) 172, 234, 242, 266, 611, 1085, 1643, 1735
BmcAI AGTACT 1 cut(s) 1520
Bme1390I CCNGG 1 cut(s) 1377
Bme18I GGWCC 3 cut(s) 527, 888, 1475
BmeT110I CYCGRG 2 cut(s) 144, 278
BmgT120I GGNCC 3 cut(s) 527, 888, 1475
BmiI GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
BmrFI CCNGG 1 cut(s) 1377
BmsI GCATC 4 cut(s) 78, 550, 985, 1226
BpiI GAAGAC 4 cut(s) 345, 430, 1293, 1944
BpmI CTGGAG 2 cut(s) 498, 1785
Bpu10I CCTNAGC 3 cut(s) 390, 1245, 1729
Bpu14I TTCGAA 1 cut(s) 379
BsaAI YACGTR 1 cut(s) 1493
BsaHI GRCGYC 3 cut(s) 134, 222, 408
BsaI GGTCTC 2 cut(s) 325, 786
BsaJI CCNNGG 4 cut(s) 259, 770, 1468, 1927
BsaWI WCCGGW 4 cut(s) 299, 325, 334, 346
Bsc4I CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1933
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 5 cut(s) 500, 530, 1526, 1576, 2003
BseAI TCCGGA 1 cut(s) 299
BseBI CCWGG 1 cut(s) 1377
BseDI CCNNGG 4 cut(s) 259, 770, 1468, 1927
BseGI GGATG 7 cut(s) 695, 757, 1000, 1129, 1228, 1444, 1630
BseLI CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1933
BseMII CTCAG 4 cut(s) 97, 404, 798, 2043
BseNI ACTGG 5 cut(s) 500, 530, 1526, 1576, 2003
BseX3I CGGCCG 1 cut(s) 262
BseXI GCAGC 5 cut(s) 182, 621, 1095, 1653, 1720
BsgI GTGCAG 1 cut(s) 1719
Bsh1236I CGCG 4 cut(s) 20, 374, 603, 712
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 1 cut(s) 264
BshNI GGYRCC 4 cut(s) 221, 242, 407, 753
BshTI ACCGGT 1 cut(s) 346
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 2 cut(s) 103, 1984
BsiHKCI CYCGRG 2 cut(s) 144, 278
BsiSI CCGG 5 cut(s) 219, 300, 326, 335, 347
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1933
BsmAI GTCTC 6 cut(s) 220, 288, 325, 786, 826, 1743
BsmBI CGTCTC 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 178
BsmI GAATGC 1 cut(s) 1541
BsnI GGCC 1 cut(s) 264
Bso31I GGTCTC 2 cut(s) 325, 786
BsoBI CYCGRG 2 cut(s) 144, 278
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 3 cut(s) 103, 1139, 1984
Bsp13I TCCGGA 1 cut(s) 299
Bsp143I GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957, 2139
Bsp19I CCATGG 2 cut(s) 770, 1468
Bsp68I TCGCGA 2 cut(s) 20, 603
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 4 cut(s) 96, 403, 799, 2044
BspEI TCCGGA 1 cut(s) 299
BspFNI CGCG 4 cut(s) 20, 374, 603, 712
BspLI GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
BspMAI CTGCAG 1 cut(s) 1714
BspMI ACCTGC 1 cut(s) 1703
BspPI GGATC 4 cut(s) 462, 713, 726, 845
BspT104I TTCGAA 1 cut(s) 379
BspT107I GGYRCC 4 cut(s) 221, 242, 407, 753
BspTNI GGTCTC 2 cut(s) 325, 786
BsrBI CCGCTC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 5 cut(s) 500, 530, 1526, 1576, 2003
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 4 cut(s) 259, 770, 1468, 1927
BssMI GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957, 2139
BssNI GRCGYC 3 cut(s) 134, 222, 408
BssSI CACGAG 2 cut(s) 184, 2012
BssT1I CCWWGG 3 cut(s) 770, 1468, 1927
Bst2BI CACGAG 2 cut(s) 184, 2012
Bst2UI CCWGG 1 cut(s) 1377
Bst4CI ACNGT 4 cut(s) 575, 586, 640, 1979
BstACI GRCGYC 3 cut(s) 134, 222, 408
BstBAI YACGTR 1 cut(s) 1493
BstBI TTCGAA 1 cut(s) 379
BstC8I GCNNGC 2 cut(s) 1081, 2000
BstDEI CTNAG 8 cut(s) 83, 390, 807, 1245, 1434, 1508, 1729, 2052
BstDSI CCRYGG 3 cut(s) 259, 770, 1468
BstF5I GGATG 7 cut(s) 695, 757, 1000, 1129, 1228, 1444, 1630
BstFNI CGCG 4 cut(s) 20, 374, 603, 712
BstH2I RGCGCY 2 cut(s) 225, 411
BstHHI GCGC 2 cut(s) 224, 410
BstKTI GATC 9 cut(s) 457, 721, 853, 1106, 1264, 1861, 1880, 1960, 2142
BstMAI GTCTC 6 cut(s) 220, 288, 325, 786, 826, 1743
BstMBI GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957, 2139
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 3 cut(s) 414, 609, 1739
BstNI CCWGG 1 cut(s) 1377
BstNSI RCATGY 2 cut(s) 769, 1801
BstSCI CCNGG 1 cut(s) 1375
BstSFI CTRYAG 2 cut(s) 1087, 1710
BstUI CGCG 4 cut(s) 20, 374, 603, 712
BstV1I GCAGC 5 cut(s) 182, 621, 1095, 1653, 1720
BstV2I GAAGAC 4 cut(s) 345, 430, 1293, 1944
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
BstZI CGGCCG 1 cut(s) 262
BsuI GTATCC 1 cut(s) 1712
BsuRI GGCC 1 cut(s) 264
BtgI CCRYGG 3 cut(s) 259, 770, 1468
BtgZI GCGATG 1 cut(s) 76
BtsCI GGATG 7 cut(s) 695, 757, 1000, 1129, 1228, 1444, 1630
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 3 cut(s) 64, 582, 1569
BtuMI TCGCGA 2 cut(s) 20, 603
BveI ACCTGC 1 cut(s) 1703
Cac8I GCNNGC 2 cut(s) 1081, 2000
CfoI GCGC 2 cut(s) 224, 410
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 3 cut(s) 527, 888, 1475
CseI GACGC 1 cut(s) 701
Csp6I GTAC 5 cut(s) 397, 503, 1519, 1949, 1968
CspAI ACCGGT 1 cut(s) 346
CviAII CATG 8 cut(s) 613, 741, 766, 771, 1058, 1469, 1798, 2036
CviQI GTAC 5 cut(s) 397, 503, 1519, 1949, 1968
DdeI CTNAG 8 cut(s) 83, 390, 807, 1245, 1434, 1508, 1729, 2052
DinI GGCGCC 2 cut(s) 223, 409
DpnI GATC 9 cut(s) 456, 720, 852, 1105, 1263, 1860, 1879, 1959, 2141
DpnII GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957, 2139
DraI TTTAAA 1 cut(s) 1503
EaeI YGGCCR 1 cut(s) 262
EagI CGGCCG 1 cut(s) 262
EciI GGCGGA 1 cut(s) 417
EclXI CGGCCG 1 cut(s) 262
Eco130I CCWWGG 3 cut(s) 770, 1468, 1927
Eco24I GRGCYC 1 cut(s) 1139
Eco31I GGTCTC 2 cut(s) 325, 786
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 3 cut(s) 527, 888, 1475
Eco52I CGGCCG 1 cut(s) 262
Eco57I CTGAAG 4 cut(s) 397, 1321, 1506, 2006
Eco72I CACGTG 1 cut(s) 1493
Eco88I CYCGRG 2 cut(s) 144, 278
EcoO109I RGGNCCY 1 cut(s) 1475
EcoRI GAATTC 3 cut(s) 616, 1033, 1283
EcoRII CCWGG 1 cut(s) 1375
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 3 cut(s) 770, 1468, 1927
EcoT38I GRGCYC 1 cut(s) 1139
EgeI GGCGCC 2 cut(s) 223, 409
EheI GGCGCC 2 cut(s) 223, 409
ErhI CCWWGG 3 cut(s) 770, 1468, 1927
Esp3I CGTCTC 1 cut(s) 220
FaeI CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1801, 2039
FaqI GGGAC 1 cut(s) 178
FatI CATG 8 cut(s) 612, 740, 765, 770, 1057, 1468, 1797, 2035
FauI CCCGC 1 cut(s) 717
FbaI TGATCA 2 cut(s) 1261, 1858
FblI GTMKAC 1 cut(s) 962
Fnu4HI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1734
FokI GGATG 7 cut(s) 702, 744, 1007, 1136, 1215, 1451, 1637
FriOI GRGCYC 1 cut(s) 1139
Fsp4HI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1734
FspBI CTAG 3 cut(s) 1419, 1527, 1650
GlaI GCGC 2 cut(s) 223, 409
GluI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1734
GsuI CTGGAG 2 cut(s) 498, 1785
HaeII RGCGCY 2 cut(s) 225, 411
HaeIII GGCC 1 cut(s) 264
HapII CCGG 5 cut(s) 219, 300, 326, 335, 347
HgaI GACGC 1 cut(s) 701
HhaI GCGC 2 cut(s) 224, 410
Hin1I GRCGYC 3 cut(s) 134, 222, 408
Hin1II CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1801, 2039
Hin6I GCGC 2 cut(s) 222, 408
HinP1I GCGC 2 cut(s) 222, 408
HincII GTYRAC 1 cut(s) 1555
HindII GTYRAC 1 cut(s) 1555
HpaI GTTAAC 1 cut(s) 1555
HpaII CCGG 5 cut(s) 219, 300, 326, 335, 347
HphI GGTGA 6 cut(s) 193, 328, 489, 1261, 1299, 1872
Hpy166II GTNNAC 4 cut(s) 77, 527, 963, 1555
Hpy188I TCNGA 8 cut(s) 86, 295, 673, 808, 850, 1222, 1986, 2139
Hpy8I GTNNAC 4 cut(s) 77, 527, 963, 1555
Hpy99I CGWCG 2 cut(s) 254, 295
HpyAV CCTTC 7 cut(s) 358, 421, 715, 1205, 1765, 1981, 2018
HpyCH4III ACNGT 4 cut(s) 575, 586, 640, 1979
HpyCH4IV ACGT 4 cut(s) 134, 565, 1492, 2098
HpyF10VI GCNNNNNNNGC 3 cut(s) 414, 609, 1739
HpyF3I CTNAG 8 cut(s) 83, 390, 807, 1245, 1434, 1508, 1729, 2052
HpySE526I ACGT 4 cut(s) 134, 565, 1492, 2098
Hsp92I GRCGYC 3 cut(s) 134, 222, 408
Hsp92II CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1801, 2039
HspAI GCGC 2 cut(s) 222, 408
KasI GGCGCC 2 cut(s) 221, 407
Kpn2I TCCGGA 1 cut(s) 299
Ksp22I TGATCA 2 cut(s) 1261, 1858
KspAI GTTAAC 1 cut(s) 1555
Kzo9I GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957, 2139
LmnI GCTCC 4 cut(s) 178, 488, 1214, 1766
Lsp1109I GCAGC 5 cut(s) 182, 621, 1095, 1653, 1720
LweI GCATC 4 cut(s) 78, 550, 985, 1226
MaeI CTAG 3 cut(s) 1419, 1527, 1650
MaeII ACGT 4 cut(s) 134, 565, 1492, 2098
MaeIII GTNAC 3 cut(s) 634, 692, 1249
MalI GATC 9 cut(s) 456, 720, 852, 1105, 1263, 1860, 1879, 1959, 2141
MbiI CCGCTC 1 cut(s) 308
MboI GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957, 2139
MfeI CAATTG 3 cut(s) 630, 873, 1195
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 3 cut(s) 103, 1139, 1984
Mly113I GGCGCC 2 cut(s) 222, 408
MlyI GAGTC 6 cut(s) 16, 81, 290, 840, 968, 1337
MmeI TCCRAC 2 cut(s) 273, 2086
MnlI CCTC 9 cut(s) 235, 278, 398, 582, 964, 1458, 1466, 1697, 1797
MroI TCCGGA 1 cut(s) 299
MspA1I CMGCKG 2 cut(s) 1484, 1644
MspI CCGG 5 cut(s) 219, 300, 326, 335, 347
MspR9I CCNGG 1 cut(s) 1377
MunI CAATTG 3 cut(s) 630, 873, 1195
Mva1269I GAATGC 1 cut(s) 1541
MvaI CCWGG 1 cut(s) 1377
MvnI CGCG 4 cut(s) 20, 374, 603, 712
MwoI GCNNNNNNNGC 3 cut(s) 414, 609, 1739
NarI GGCGCC 2 cut(s) 222, 408
NcoI CCATGG 2 cut(s) 770, 1468
NdeII GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957, 2139
NlaIII CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1801, 2039
NlaIV GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 2 cut(s) 692, 1249
NruI TCGCGA 2 cut(s) 20, 603
NspI RCATGY 2 cut(s) 769, 1801
NspV TTCGAA 1 cut(s) 379
PaeR7I CTCGAG 1 cut(s) 278
PaqCI CACCTGC 1 cut(s) 1703
PciI ACATGT 1 cut(s) 1797
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 571
PctI GAATGC 1 cut(s) 1541
PfeI GAWTC 8 cut(s) 254, 296, 491, 668, 866, 940, 1664, 1826
PflMI CCANNNNNTGG 2 cut(s) 521, 1933
PinAI ACCGGT 1 cut(s) 346
PkrI GCNGC 8 cut(s) 172, 234, 242, 266, 611, 1085, 1643, 1735
PleI GAGTC 6 cut(s) 16, 81, 289, 839, 967, 1336
PluTI GGCGCC 2 cut(s) 225, 411
PmaCI CACGTG 1 cut(s) 1493
PmlI CACGTG 1 cut(s) 1493
PpsI GAGTC 6 cut(s) 16, 81, 289, 839, 967, 1336
Ppu21I YACGTR 1 cut(s) 1493
PpuMI RGGWCCY 1 cut(s) 1475
PscI ACATGT 1 cut(s) 1797
PshBI ATTAAT 1 cut(s) 1352
PsiI TTATAA 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 1475
Psp6I CCWGG 1 cut(s) 1375
PspCI CACGTG 1 cut(s) 1493
PspGI CCWGG 1 cut(s) 1375
PspN4I GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
PspPI GGNCC 3 cut(s) 527, 888, 1475
PspPPI RGGWCCY 1 cut(s) 1475
PspXI VCTCGAGB 1 cut(s) 278
PstI CTGCAG 1 cut(s) 1714
PsuI RGATCY 1 cut(s) 718
PvuII CAGCTG 2 cut(s) 1484, 1644
RruI TCGCGA 2 cut(s) 20, 603
RsaI GTAC 5 cut(s) 398, 504, 1520, 1950, 1969
RsaNI GTAC 5 cut(s) 397, 503, 1519, 1949, 1968
SatI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1734
Sau3AI GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957, 2139
Sau96I GGNCC 3 cut(s) 527, 888, 1475
ScaI AGTACT 1 cut(s) 1520
SchI GAGTC 6 cut(s) 16, 81, 290, 840, 968, 1337
ScrFI CCNGG 1 cut(s) 1377
SduI GDGCHC 3 cut(s) 103, 1139, 1984
SfaNI GCATC 4 cut(s) 78, 550, 985, 1226
SfcI CTRYAG 2 cut(s) 1087, 1710
SfoI GGCGCC 2 cut(s) 223, 409
Sfr274I CTCGAG 1 cut(s) 278
SfuI TTCGAA 1 cut(s) 379
SinI GGWCC 3 cut(s) 527, 888, 1475
SlaI CTCGAG 1 cut(s) 278
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SpeI ACTAGT 1 cut(s) 1526
SspDI GGCGCC 2 cut(s) 221, 407
SspI AATATT 2 cut(s) 1156, 1180
SspMI CTAG 3 cut(s) 1419, 1527, 1650
StyD4I CCNGG 1 cut(s) 1375
StyI CCWWGG 3 cut(s) 770, 1468, 1927
TaaI ACNGT 4 cut(s) 575, 586, 640, 1979
TaiI ACGT 4 cut(s) 137, 568, 1495, 2101
TaqI TCGA 4 cut(s) 252, 279, 379, 1747
TatI WGTACW 1 cut(s) 1518
TauI GCSGC 3 cut(s) 235, 243, 267
TfiI GAWTC 8 cut(s) 254, 296, 491, 668, 866, 940, 1664, 1826
TscAI CASTG 3 cut(s) 71, 589, 1576
TseFI GTSAC 2 cut(s) 692, 1249
TseI GCWGC 5 cut(s) 170, 609, 1083, 1641, 1733
Tsp45I GTSAC 2 cut(s) 692, 1249
TspDTI ATGAA 7 cut(s) 629, 901, 1056, 1074, 1217, 1455, 2037
TspGWI ACGGA 2 cut(s) 279, 1181
TspRI CASTG 3 cut(s) 71, 589, 1576
Van91I CCANNNNNTGG 2 cut(s) 521, 1933
VpaK11BI GGWCC 3 cut(s) 527, 888, 1475
VspI ATTAAT 1 cut(s) 1352
XapI RAATTY 8 cut(s) 616, 1001, 1033, 1043, 1283, 1497, 1820, 1901
XceI RCATGY 2 cut(s) 769, 1801
XhoI CTCGAG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 962
XspI CTAG 3 cut(s) 1419, 1527, 1650
ZraI GACGTC 1 cut(s) 135
ZrmI AGTACT 1 cut(s) 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.