MD08G1107200.v1.1

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012367
Physical Location & Seq
Reverse (-)
9405980 .. 9411350
5371 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1107200.v1.1.491

Sequence Viewer

Length: 2208 bp
ATGAGGATGGAGGGCAGAGAAGCAGGTCCGTTATTTTTAAAATATAAAAACGCGGATTGGGTGATTCAGAAAACCATCACTCACACTCGCTTATCTTCGTCTTCCTTCAATCGAATCCGTCAACGATTCAGCTCCAGAGTCCAGACGAACAGCCACCGCCGCCACCAAATGACTACCTCTTCTTCATTGGAAGCCAAGCAATTGGAGAAAATCGTAGCTCCGTACGGGTCCTGGAAATCCCCAATCTCCGCCGACGTCGTCTCCGGCGCCTCCAAGCGACTCGGCGGCACGGCCGTCGATTCCTTTGGCCGACTCATCTGGCTCGAGTCCCGCCCCTCCGAATCAGGACGGGCCGTTCTGGTGAGAGAATCGGAGAGGCCGGAGGGCGAACCGGTTGATATTACACCGAAGGACTATGCGGTTCGGACGGTTGCGCAGGAGTACGGAGGTGGTGCATTCAGCGTTTCAGGGGATACAATCATTTTCTCCAATTACAGGGATCAACGTCTCTACAAGCAATCTCTAAGTTCCAATGATTCTACTCCTACACCACTCACACCGGATTATGGCGGGCCGGTTATTAGTTATGCTGACGGGGTGTTTGATGCACGTTTTAACCGTTTTGTCACTGTACAGGAAGATCGTCGTGAAAGCAGCATTAATTCGATAACAACAATTGTTTCAGTAGGGATTGATGGGAAGGACATTCGGGAACCAGAAGTACTAGTGGGTGGAACGGATTTTTATGCCTTCCCGCGTTTGGATCCCAAAGGTGAACGGCTGGCATGGATTGAATGGTGCCATCCTAACATGCCATGGGATCAGTCTCAACTTTGGGTTGGGTATATCTCTGAAAAGGGAGAAATCTACCAACGAATTTGTGTTGCTGGCTCTGATCCTTCCATGAAGGAGTCCCCAATTGAACCCAAGTGGTCCTCTAAAGGGGAACTATTTTTTATTACTGACAGAAAAAGTGGGTACTGGAATCTTTACAAATGGGTTGAGTCTAGTAATGAGGTCATATCAGTTTATTCCTTGGATGCTGAGTTTTCCAAACCATTATGGGTTTTTGGCATGAATTCTTATGAATTTATTCAGAGCCATGAAAGGAAAAACATAATTGCTTGCAGCTATAGGCAGAAGGGGAGGTCATATCTTGGAATCCTGGATGATGTGCACGGATCACTATCTTTGCTTAATACTCCTTTTACAGATATTGATAATATTACCTTGGGGATCAATTGCCTGTACGTGGAAGGAGCATCAGAAGTTCATCCGTCGGCTGTTGCTAAGGTGACTTTAGACAACCATAAAATGAAAGCAGTTGACTTCAGGATTATTTGGTCTTCTTCACCTGATTGCTTAAAATACCAGTCCTACTTTAGCCTACCTGAATTCATTGAATTCCCAACAGAAGTTCCAGGTCAAACTGCCTTTGCATACTTTTACCCACCATCAAATCCCAATTACCAAGCTCCTCAGGGTGAAAAGCCTCCACTGTTATTGGAAAGCCATGGAGGGCCTACCTCTGAATCCCATGGAATCTTAAATCTGAGTATTCAGTACTGGACGAGTCGTGGTTGGGCATATGTTAATGTTAACTATGGTGGAAGCACTGGATATGGCAGGGAATATCGGGAACGGCTCTTGAACAGATGGGGAATTGTGGATGTGAATGATTGTTGCAGCTGTGCTAGATATCTGGTGGATTCCGGAAAGGTAGATGGGGAACGACTTTGTATTACTGGGGGCTCTGCAGGTGGCTACACAACCTTAGCTGCGCTTGCTTTTAGAGACACCTTCAAGGCAGGATCTTCCTTGTGTGGTGTAGCTGACTTAAACATGTTGAGGGCAGAAACTCATAAATTTGAATCTCATTATCTTGATAATCTCGTTGGCAGTGAAAAGGATTACTATGATAGGTCACCCATCAACTTTGTCGATAAGTTTTCTTGCCCGATAATCTTATTCCAAGGGTTGGAAGACAAGGTTGTACCCCCAGATCAAGCTCGTAAAATTTACCACGCTTTGAAGGAAAAGGGTTTGCCTGTTGCTCATGTGGAGTATGAAGGAGAACAGCACGGCTTCCGCAAGGCCGAGAACATTAAGTTCACGCTGGAACAGCAAATGGTTTTCTTTGCACGTTTAGTTGGACACTTCAAGGTTGCGGACGAGATTAATCCGATCAAAATTGATAACTTCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

736

Amino Acids

82.85

Weight (kDa)

6.04

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 519 - 718 7.6e-47 Prolyl oligopeptidase family
BD-FAE PF20434 521 - 677 1.7e-08 BD-FAE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36210
fragaria_vesca FvH4_2g34950
malus_domestica MD08G1107000.v1.1 MD08G1107200.v1.1 MD15G1087700.v1.1 MD15G1087800.v1.1
prunus_persica Prupe.1G441500_v2.0.a1
pyrus_communis pycom08g08900 pycom08g08910 pycom15g08240
rosa_chinensis RchiOBHm_Chr6g0307051
rosa_laevigata RLG00000010738
rosa_multiflora Rmu_sc0005634.1_g000006
rosa_roxburghii Rroxscaffold_7G00161200
rosa_rugosa Rorug06G0355500
rosa_samantha Rh6AG467000 Rh6CG482500 Rh6DG468800
rosa_wichuraiana Rw6G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1748
AatII GACGTC 1 cut(s) 258
Acc16I TGCGCA 1 cut(s) 435
Acc36I ACCTGC 2 cut(s) 14, 1748
AccB1I GGYRCC 2 cut(s) 266, 798
AccB7I CCANNNNNTGG 1 cut(s) 1978
AccII CGCG 2 cut(s) 53, 757
AccIII TCCGGA 1 cut(s) 1712
AclWI GGATC 8 cut(s) 507, 758, 771, 828, 890, 1189, 1244, 1819
AcoI YGGCCR 2 cut(s) 291, 307
AcsI RAATTY 7 cut(s) 876, 1078, 1088, 1394, 1403, 1865, 2016
AcuI CTGAAG 1 cut(s) 1315
AcyI GRCGYC 2 cut(s) 255, 267
AfaI GTAC 8 cut(s) 224, 443, 633, 723, 980, 1250, 1565, 1995
AfiI CCNNNNNNNGG 6 cut(s) 495, 566, 760, 942, 1252, 1978
AflIII ACRYGT 1 cut(s) 1842
AgeI ACCGGT 1 cut(s) 391
AgsI TTSAA 9 cut(s) 109, 794, 923, 1403, 1651, 1804, 1871, 2032, 2161
AhlI ACTAGT 1 cut(s) 724
AjnI CCWGG 3 cut(s) 230, 1164, 1420
AloI GAACNNNNNNTCC 2 cut(s) 339, 371
AluBI AGCT 8 cut(s) 132, 218, 1131, 1475, 1689, 1778, 1832, 2009
AluI AGCT 8 cut(s) 132, 218, 1131, 1475, 1689, 1778, 1832, 2009
Alw21I GWGCWC 1 cut(s) 1179
Alw26I GTCTC 4 cut(s) 265, 512, 831, 1788
Alw44I GTGCAC 1 cut(s) 1175
AlwI GGATC 8 cut(s) 507, 758, 771, 828, 890, 1189, 1244, 1819
Ama87I CYCGRG 1 cut(s) 323
Aor13HI TCCGGA 1 cut(s) 1712
AoxI GGCC 7 cut(s) 291, 307, 351, 377, 572, 1520, 2094
ApaLI GTGCAC 1 cut(s) 1175
ApeKI GCWGC 4 cut(s) 654, 1128, 1686, 1778
ApoI RAATTY 7 cut(s) 876, 1078, 1088, 1394, 1403, 1865, 2016
ArsI GACNNNNNNTTYG 2 cut(s) 584, 616
AseI ATTAAT 2 cut(s) 660, 2178
AsiGI ACCGGT 1 cut(s) 391
Asp700I GAANNNNTTC 1 cut(s) 1092
AspLEI GCGC 3 cut(s) 269, 436, 1783
AspS9I GGNCC 6 cut(s) 26, 228, 351, 572, 933, 1520
AsuHPI GGTGA 7 cut(s) 73, 373, 785, 1306, 1344, 1496, 1917
AvaI CYCGRG 1 cut(s) 323
AvaII GGWCC 3 cut(s) 26, 228, 933
AxyI CCTNAGG 1 cut(s) 1479
BaeGI GKGCMC 1 cut(s) 1179
BamHI GGATCC 1 cut(s) 763
BanI GGYRCC 2 cut(s) 266, 798
BanII GRGCYC 1 cut(s) 1754
BbsI GAAGAC 3 cut(s) 93, 1338, 1989
Bbv12I GWGCWC 1 cut(s) 1179
BbvI GCAGC 4 cut(s) 666, 1140, 1698, 1765
BccI CCATC 7 cut(s) 83, 689, 810, 1462, 1650, 1718, 1937
BceAI ACGGC 6 cut(s) 278, 306, 338, 794, 1658, 2098
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciT130I CCWGG 3 cut(s) 232, 1166, 1422
BciVI GTATCC 1 cut(s) 466
BcoDI GTCTC 4 cut(s) 265, 512, 831, 1788
BcuI ACTAGT 1 cut(s) 724
BfaI CTAG 3 cut(s) 725, 1008, 1695
BfmI CTRYAG 2 cut(s) 1132, 1755
BfoI RGCGCY 1 cut(s) 270
BfuAI ACCTGC 2 cut(s) 14, 1748
BfuI GTATCC 1 cut(s) 466
BisI GCNGC 6 cut(s) 160, 286, 655, 1129, 1687, 1779
BlsI GCNGC 6 cut(s) 161, 287, 656, 1130, 1688, 1780
BmcAI AGTACT 2 cut(s) 723, 1565
Bme1390I CCNGG 3 cut(s) 232, 1166, 1422
Bme18I GGWCC 3 cut(s) 26, 228, 933
BmeT110I CYCGRG 1 cut(s) 323
BmgT120I GGNCC 6 cut(s) 26, 228, 351, 572, 933, 1520
BmiI GGNNCC 5 cut(s) 229, 268, 714, 765, 800
BmrFI CCNGG 3 cut(s) 232, 1166, 1422
BmrI ACTGGG 1 cut(s) 1755
BmsI GCATC 3 cut(s) 595, 1030, 1271
BmuI ACTGGG 1 cut(s) 1755
BpiI GAAGAC 3 cut(s) 93, 1338, 1989
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 2 cut(s) 1290, 1774
BsaAI YACGTR 1 cut(s) 1252
BsaBI GATNNNNATC 1 cut(s) 1186
BsaHI GRCGYC 2 cut(s) 255, 267
BsaJI CCNNGG 6 cut(s) 815, 1035, 1230, 1513, 1537, 1972
BsaWI WCCGGW 3 cut(s) 391, 559, 1712
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 6 cut(s) 495, 566, 760, 942, 1252, 1978
Bse118I RCCGGY 2 cut(s) 391, 574
Bse1I ACTGG 5 cut(s) 986, 1372, 1571, 1621, 1750
Bse21I CCTNAGG 1 cut(s) 1479
Bse8I GATNNNNATC 1 cut(s) 1186
BseAI TCCGGA 1 cut(s) 1712
BseBI CCWGG 3 cut(s) 232, 1166, 1422
BseDI CCNNGG 6 cut(s) 815, 1035, 1230, 1513, 1537, 1972
BseGI GGATG 6 cut(s) 12, 802, 1045, 1174, 1273, 1675
BseJI GATNNNNATC 1 cut(s) 1186
BseLI CCNNNNNNNGG 6 cut(s) 495, 566, 760, 942, 1252, 1978
BseMII CTCAG 3 cut(s) 1035, 1493, 1544
BseNI ACTGG 5 cut(s) 986, 1372, 1571, 1621, 1750
BseRI GAGGAG 1 cut(s) 1467
BseSI GKGCMC 1 cut(s) 1179
BseX3I CGGCCG 1 cut(s) 291
BseXI GCAGC 4 cut(s) 666, 1140, 1698, 1765
Bsh1236I CGCG 2 cut(s) 53, 757
Bsh1285I CGRYCG 1 cut(s) 294
BshFI GGCC 7 cut(s) 293, 309, 353, 379, 574, 1522, 2096
BshNI GGYRCC 2 cut(s) 266, 798
BshTI ACCGGT 1 cut(s) 391
BsiEI CGRYCG 1 cut(s) 294
BsiHKAI GWGCWC 1 cut(s) 1179
BsiHKCI CYCGRG 1 cut(s) 323
BsiSI CCGG 6 cut(s) 264, 380, 392, 560, 575, 1713
BsiWI CGTACG 1 cut(s) 222
BslFI GGGAC 2 cut(s) 313, 898
BslI CCNNNNNNNGG 6 cut(s) 495, 566, 760, 942, 1252, 1978
BsmAI GTCTC 4 cut(s) 265, 512, 831, 1788
BsmBI CGTCTC 2 cut(s) 265, 512
BsmFI GGGAC 2 cut(s) 313, 898
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 7 cut(s) 293, 309, 353, 379, 574, 1522, 2096
BsoBI CYCGRG 1 cut(s) 323
Bsp1286I GDGCHC 2 cut(s) 1179, 1754
Bsp13I TCCGGA 1 cut(s) 1712
Bsp1407I TGTACA 1 cut(s) 631
Bsp19I CCATGG 3 cut(s) 815, 1513, 1537
BspANI GGCC 7 cut(s) 293, 309, 353, 379, 574, 1522, 2096
BspCNI CTCAG 3 cut(s) 1036, 1492, 1545
BspEI TCCGGA 1 cut(s) 1712
BspFNI CGCG 2 cut(s) 53, 757
BspLI GGNNCC 5 cut(s) 229, 268, 714, 765, 800
BspMAI CTGCAG 1 cut(s) 1759
BspMI ACCTGC 2 cut(s) 14, 1748
BspPI GGATC 8 cut(s) 507, 758, 771, 828, 890, 1189, 1244, 1819
BspT107I GGYRCC 2 cut(s) 266, 798
BsrFI RCCGGY 2 cut(s) 391, 574
BsrGI TGTACA 1 cut(s) 631
BsrI ACTGG 5 cut(s) 986, 1372, 1571, 1621, 1750
BssAI RCCGGY 2 cut(s) 391, 574
BssECI CCNNGG 6 cut(s) 815, 1035, 1230, 1513, 1537, 1972
BssNI GRCGYC 2 cut(s) 255, 267
BssT1I CCWWGG 6 cut(s) 815, 1035, 1230, 1513, 1537, 1972
Bst2UI CCWGG 3 cut(s) 232, 1166, 1422
Bst4CI ACNGT 4 cut(s) 430, 620, 631, 1500
Bst6I CTCTTC 1 cut(s) 184
BstACI GRCGYC 2 cut(s) 255, 267
BstAUI TGTACA 1 cut(s) 631
BstBAI YACGTR 1 cut(s) 1252
BstC8I GCNNGC 5 cut(s) 572, 783, 889, 1126, 1785
BstDEI CTNAG 6 cut(s) 524, 1044, 1290, 1479, 1553, 1774
BstDSI CCRYGG 3 cut(s) 815, 1513, 1537
BstEII GGTNACC 1 cut(s) 1923
BstF5I GGATG 6 cut(s) 12, 802, 1045, 1174, 1273, 1675
BstFNI CGCG 2 cut(s) 53, 757
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 3 cut(s) 269, 436, 1783
BstMAI GTCTC 4 cut(s) 265, 512, 831, 1788
BstMCI CGRYCG 1 cut(s) 294
BstMWI GCNNNNNNNGC 3 cut(s) 159, 1784, 2122
BstNI CCWGG 3 cut(s) 232, 1166, 1422
BstNSI RCATGY 2 cut(s) 814, 1846
BstPI GGTNACC 1 cut(s) 1923
BstSCI CCNGG 3 cut(s) 230, 1164, 1420
BstSFI CTRYAG 2 cut(s) 1132, 1755
BstSLI GKGCMC 1 cut(s) 1179
BstUI CGCG 2 cut(s) 53, 757
BstV1I GCAGC 4 cut(s) 666, 1140, 1698, 1765
BstV2I GAAGAC 3 cut(s) 93, 1338, 1989
BstX2I RGATCY 2 cut(s) 763, 1811
BstXI CCANNNNNNTGG 1 cut(s) 202
BstYI RGATCY 2 cut(s) 763, 1811
BstZI CGGCCG 1 cut(s) 291
Bsu36I CCTNAGG 1 cut(s) 1479
BsuI GTATCC 1 cut(s) 466
BsuRI GGCC 7 cut(s) 293, 309, 353, 379, 574, 1522, 2096
BtgI CCRYGG 3 cut(s) 815, 1513, 1537
BtsCI GGATG 6 cut(s) 12, 802, 1045, 1174, 1273, 1675
BtsI GCAGTG 1 cut(s) 1906
BtsIMutI CAGTG 4 cut(s) 627, 1496, 1614, 1906
BveI ACCTGC 2 cut(s) 14, 1748
Cac8I GCNNGC 5 cut(s) 572, 783, 889, 1126, 1785
CfoI GCGC 3 cut(s) 269, 436, 1783
Cfr10I RCCGGY 2 cut(s) 391, 574
Cfr13I GGNCC 6 cut(s) 26, 228, 351, 572, 933, 1520
Csp6I GTAC 8 cut(s) 223, 442, 632, 722, 979, 1249, 1564, 1994
CspAI ACCGGT 1 cut(s) 391
CspCI CAANNNNNGTGG 2 cut(s) 1485, 1520
CviQI GTAC 8 cut(s) 223, 442, 632, 722, 979, 1249, 1564, 1994
DdeI CTNAG 6 cut(s) 524, 1044, 1290, 1479, 1553, 1774
DinI GGCGCC 1 cut(s) 268
DraI TTTAAA 1 cut(s) 39
EaeI YGGCCR 2 cut(s) 291, 307
EagI CGGCCG 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 184
EciI GGCGGA 1 cut(s) 238
EclXI CGGCCG 1 cut(s) 291
Eco130I CCWWGG 6 cut(s) 815, 1035, 1230, 1513, 1537, 1972
Eco24I GRGCYC 1 cut(s) 1754
Eco32I GATATC 1 cut(s) 1700
Eco47I GGWCC 3 cut(s) 26, 228, 933
Eco52I CGGCCG 1 cut(s) 291
Eco57I CTGAAG 1 cut(s) 1315
Eco81I CCTNAGG 1 cut(s) 1479
Eco88I CYCGRG 1 cut(s) 323
Eco91I GGTNACC 1 cut(s) 1923
EcoO109I RGGNCCY 2 cut(s) 228, 1520
EcoO65I GGTNACC 1 cut(s) 1923
EcoRI GAATTC 3 cut(s) 1078, 1394, 1403
EcoRII CCWGG 3 cut(s) 230, 1164, 1420
EcoRV GATATC 1 cut(s) 1700
EcoT14I CCWWGG 6 cut(s) 815, 1035, 1230, 1513, 1537, 1972
EcoT38I GRGCYC 1 cut(s) 1754
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
ErhI CCWWGG 6 cut(s) 815, 1035, 1230, 1513, 1537, 1972
Esp3I CGTCTC 2 cut(s) 265, 512
FaqI GGGAC 2 cut(s) 313, 898
FauI CCCGC 3 cut(s) 338, 563, 762
FauNDI CATATG 1 cut(s) 1588
Fnu4HI GCNGC 6 cut(s) 160, 286, 655, 1129, 1687, 1779
FokI GGATG 6 cut(s) 19, 789, 1052, 1181, 1260, 1682
FriOI GRGCYC 1 cut(s) 1754
Fsp4HI GCNGC 6 cut(s) 160, 286, 655, 1129, 1687, 1779
FspBI CTAG 3 cut(s) 725, 1008, 1695
FspI TGCGCA 1 cut(s) 435
GlaI GCGC 3 cut(s) 268, 435, 1782
GluI GCNGC 6 cut(s) 160, 286, 655, 1129, 1687, 1779
GsuI CTGGAG 1 cut(s) 118
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 7 cut(s) 293, 309, 353, 379, 574, 1522, 2096
HapII CCGG 6 cut(s) 264, 380, 392, 560, 575, 1713
HhaI GCGC 3 cut(s) 269, 436, 1783
Hin1I GRCGYC 2 cut(s) 255, 267
Hin6I GCGC 3 cut(s) 267, 434, 1781
HinP1I GCGC 3 cut(s) 267, 434, 1781
HincII GTYRAC 3 cut(s) 122, 1327, 1600
HindII GTYRAC 3 cut(s) 122, 1327, 1600
HpaI GTTAAC 1 cut(s) 1600
HpaII CCGG 6 cut(s) 264, 380, 392, 560, 575, 1713
HphI GGTGA 7 cut(s) 73, 373, 785, 1306, 1344, 1496, 1917
Hpy166II GTNNAC 6 cut(s) 122, 776, 1177, 1327, 1600, 2112
Hpy8I GTNNAC 6 cut(s) 122, 776, 1177, 1327, 1600, 2112
Hpy99I CGWCG 5 cut(s) 257, 260, 299, 648, 1282
HpyCH4III ACNGT 4 cut(s) 430, 620, 631, 1500
HpyCH4IV ACGT 5 cut(s) 255, 505, 610, 1251, 2143
HpyCH4V TGCA 8 cut(s) 455, 608, 1128, 1177, 1439, 1686, 1757, 2141
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 1784, 2122
HpyF3I CTNAG 6 cut(s) 524, 1044, 1290, 1479, 1553, 1774
HpySE526I ACGT 5 cut(s) 255, 505, 610, 1251, 2143
Hsp92I GRCGYC 2 cut(s) 255, 267
HspAI GCGC 3 cut(s) 267, 434, 1781
KasI GGCGCC 1 cut(s) 266
Kpn2I TCCGGA 1 cut(s) 1712
KspAI GTTAAC 1 cut(s) 1600
LmnI GCTCC 4 cut(s) 137, 223, 1259, 1480
Lsp1109I GCAGC 4 cut(s) 666, 1140, 1698, 1765
LweI GCATC 3 cut(s) 595, 1030, 1271
MaeI CTAG 3 cut(s) 725, 1008, 1695
MaeII ACGT 5 cut(s) 255, 505, 610, 1251, 2143
MaeIII GTNAC 3 cut(s) 625, 1294, 1923
MboII GAAGA 9 cut(s) 87, 93, 171, 174, 650, 1338, 1341, 1806, 1994
MfeI CAATTG 4 cut(s) 200, 675, 918, 1240
MflI RGATCY 2 cut(s) 763, 1811
MhlI GDGCHC 2 cut(s) 1179, 1754
Mly113I GGCGCC 1 cut(s) 267
MlyI GAGTC 7 cut(s) 147, 273, 306, 335, 920, 1013, 1582
MmeI TCCRAC 2 cut(s) 1959, 2131
MroI TCCGGA 1 cut(s) 1712
MroXI GAANNNNTTC 1 cut(s) 1092
MspA1I CMGCKG 1 cut(s) 1689
MspI CCGG 6 cut(s) 264, 380, 392, 560, 575, 1713
MspR9I CCNGG 3 cut(s) 232, 1166, 1422
MunI CAATTG 4 cut(s) 200, 675, 918, 1240
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 3 cut(s) 232, 1166, 1422
MvnI CGCG 2 cut(s) 53, 757
MwoI GCNNNNNNNGC 3 cut(s) 159, 1784, 2122
NarI GGCGCC 1 cut(s) 267
NcoI CCATGG 3 cut(s) 815, 1513, 1537
NdeI CATATG 1 cut(s) 1588
NlaIV GGNNCC 5 cut(s) 229, 268, 714, 765, 800
NmeAIII GCCGAG 2 cut(s) 261, 2122
NmuCI GTSAC 3 cut(s) 625, 1294, 1923
NsbI TGCGCA 1 cut(s) 435
NspI RCATGY 2 cut(s) 814, 1846
PaeR7I CTCGAG 1 cut(s) 323
PaqCI CACCTGC 1 cut(s) 1748
PciI ACATGT 1 cut(s) 1842
PcsI WCGNNNNNNNCGW 1 cut(s) 616
PctI GAATGC 1 cut(s) 455
PdmI GAANNNNTTC 1 cut(s) 1092
Pfl23II CGTACG 1 cut(s) 222
PflFI GACNNNGTC 1 cut(s) 257
PflMI CCANNNNNTGG 1 cut(s) 1978
PfoI TCCNGGA 2 cut(s) 230, 1164
PinAI ACCGGT 1 cut(s) 391
PkrI GCNGC 6 cut(s) 161, 287, 656, 1130, 1688, 1780
PleI GAGTC 7 cut(s) 146, 273, 306, 334, 919, 1012, 1581
PluTI GGCGCC 1 cut(s) 270
PpsI GAGTC 7 cut(s) 146, 273, 306, 334, 919, 1012, 1581
Ppu21I YACGTR 1 cut(s) 1252
PpuMI RGGWCCY 1 cut(s) 228
PscI ACATGT 1 cut(s) 1842
PshBI ATTAAT 2 cut(s) 660, 2178
Psp5II RGGWCCY 1 cut(s) 228
Psp6I CCWGG 3 cut(s) 230, 1164, 1420
PspEI GGTNACC 1 cut(s) 1923
PspGI CCWGG 3 cut(s) 230, 1164, 1420
PspLI CGTACG 1 cut(s) 222
PspN4I GGNNCC 5 cut(s) 229, 268, 714, 765, 800
PspPI GGNCC 6 cut(s) 26, 228, 351, 572, 933, 1520
PspPPI RGGWCCY 1 cut(s) 228
PspXI VCTCGAGB 1 cut(s) 323
PsrI GAACNNNNNNTAC 2 cut(s) 705, 737
PstI CTGCAG 1 cut(s) 1759
PsuI RGATCY 2 cut(s) 763, 1811
PsyI GACNNNGTC 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 1689
RsaI GTAC 8 cut(s) 224, 443, 633, 723, 980, 1250, 1565, 1995
RsaNI GTAC 8 cut(s) 223, 442, 632, 722, 979, 1249, 1564, 1994
SatI GCNGC 6 cut(s) 160, 286, 655, 1129, 1687, 1779
Sau96I GGNCC 6 cut(s) 26, 228, 351, 572, 933, 1520
ScaI AGTACT 2 cut(s) 723, 1565
SchI GAGTC 7 cut(s) 147, 273, 306, 335, 920, 1013, 1582
ScrFI CCNGG 3 cut(s) 232, 1166, 1422
SduI GDGCHC 2 cut(s) 1179, 1754
SfaNI GCATC 3 cut(s) 595, 1030, 1271
SfcI CTRYAG 2 cut(s) 1132, 1755
SfoI GGCGCC 1 cut(s) 268
Sfr274I CTCGAG 1 cut(s) 323
SinI GGWCC 3 cut(s) 26, 228, 933
SlaI CTCGAG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 323
SmoI CTYRAG 1 cut(s) 323
SpeI ACTAGT 1 cut(s) 724
SspDI GGCGCC 1 cut(s) 266
SspI AATATT 1 cut(s) 1225
SspMI CTAG 3 cut(s) 725, 1008, 1695
StyD4I CCNGG 3 cut(s) 230, 1164, 1420
StyI CCWWGG 6 cut(s) 815, 1035, 1230, 1513, 1537, 1972
TaaI ACNGT 4 cut(s) 430, 620, 631, 1500
TaiI ACGT 5 cut(s) 258, 508, 613, 1254, 2146
TaqI TCGA 6 cut(s) 112, 297, 324, 665, 1941, 2202
TatI WGTACW 3 cut(s) 631, 721, 1563
TauI GCSGC 2 cut(s) 162, 288
TscAI CASTG 4 cut(s) 634, 1503, 1621, 1906
TseFI GTSAC 3 cut(s) 625, 1294, 1923
TseI GCWGC 4 cut(s) 654, 1128, 1686, 1778
Tsp45I GTSAC 3 cut(s) 625, 1294, 1923
TspDTI ATGAA 9 cut(s) 174, 920, 1091, 1101, 1119, 1262, 1331, 1387, 2082
TspGWI ACGGA 7 cut(s) 18, 107, 210, 459, 752, 1194, 1266
TspRI CASTG 4 cut(s) 634, 1503, 1621, 1906
Tth111I GACNNNGTC 1 cut(s) 257
Van91I CCANNNNNTGG 1 cut(s) 1978
VneI GTGCAC 1 cut(s) 1175
VpaK11BI GGWCC 3 cut(s) 26, 228, 933
VspI ATTAAT 2 cut(s) 660, 2178
XapI RAATTY 7 cut(s) 876, 1078, 1088, 1394, 1403, 1865, 2016
XceI RCATGY 2 cut(s) 814, 1846
XhoI CTCGAG 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 1092
XspI CTAG 3 cut(s) 725, 1008, 1695
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 2 cut(s) 723, 1565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.