Rw6G040710

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012367
Physical Location & Seq
Forward (+)
64480333 .. 64485885
5553 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040710.1

Sequence Viewer

Length: 2031 bp
ATGGCTCTCTCGTCCATCACCAGTCTCACTCGCTTCTCTACTACTACTACTTCTTCTCACTTCCACTGCATCAGTAAACGGCTCAGACTCATCGCCAGAGCACCAACCAAAAGTCAGCACCGCCAGAAAATGACGTCATCTTCTCCGGAAGCCAAGCAAGAGAAAATCGCAGCTCCTTTCGGCTCGTGGAAGTCCCCAATCACCGCCGATGTCGTCTCCGGCGCCTCCAAGCGGCTCGGCGGCACCGCCGTCGATTCCCACGGCCGCCTCATCTGGCTCGAGTCTCGTCCGTCGGAATCCGGACGAGCGGTTCTGGTGGGAGAACCGGAGAGACCGGAAGACGAACCGGTTGATATCACGCCGAAGGACTACGCGGTTCGAACTCTGGCTCAGGAGTACGGCGGAGGCGCCTTCAGCGTTTCAGGAGATACACTTGTCTTCTCAAATTATAAGGATCAACGCCTTTACAAGCAGTCTTTAAGCTCCAAAGATTCTTCACCAGTACCACTCACTCCAGATTATGGTGGACCAGTTGTTAGTTATGCTGATGGGGTGTTTGATGCACGTTTTAACCGTTTTATCACTGTGAGGGAAGATTGTCGCGAAAGCAGCATGAATTCAATCACAACAATTGTAACAGTAAGGCTTGATGGAAGGGACATTCAGGAATCAGAAACACTCGTAGGTGGATGTGACTTCTATGCCTTCCCGCGTCTGGATCCCAAAGGAGAACGACTTGCATGGATTGAATGGTGCCATCCTAACATGCCATGGGATAGGTCTCAACTTTGGGTTGGGTATATATCTGAGAAAGGGGAAGTCTCAAAACGAGTCTGTGTTTCTGGCTCTGATCCTGCAATTAAGGAATCTCCAATTGAACCCAAGTGGTCCGCAAAAGGGGAACTATTTTTCATTACTGACAGAAAAAGTGGGTTTTGGAATCTCTATAAATGGGTTGAGTCTACTAATGAGGTTGTATCAGTTTATTCTTTGGATGCTGAATTTTCCAAACCATTATGGGTTTTTGGGTTGAATTCTTATGAATTTATTGAGAGCCATGAACAAAAGAACCTTATTGCTTGCAGCTATAGGAAAAACGGGAGATCATATCTTGGAATTGTGGATGATATTCAGGGCTCATTATCCCTGCTTAATATTCCTTTCACGGATATTGATAATATTACTTTGGGGTTCAATTGCCTATATGTGGAAGGAGCATCAGAAGTTCATCCATTGTCTGTTGCTAAGGTGACATTAGATGATCAAAAATCAAAAGCAGTTGAATTCAAGATTATTTGGTCTTCTTCACCCGATTGCTTAAAATATGAGTCCTACTTCAGCCTACCTGAATTAATTGAGTTCCCAACAGAAGTTCCTGGTCAAACTGCCTTTGCATACTTTTATCCACCATCAAATCCTAGCTACCAAGCAACTAAGGATGAAAAGCCTCCACTATTACTGAAAAGCCATGGAGGACCCACAGCTGAAGCACGTGGAATTTTAAATCTTAGTGTTCAGTTCTGGACTAGTCGTGGTTGGGCATTCGTTGATGTTAACTACGGTGGAAGCACTGGTTATGGCAGAGAATATCGTGAAAGGCTGTTGAAGAAATGGGGAATTGTGGATGTAAATGATTGTTGCAGCTGTGCTAGTTATCTGGTGGAATCTGGAAAGGTAGATGGGAAACGACTTTGTATTACTGGAGGCTCTGCAGGTGGATACACAACCTTAGCGGCACTTGCATTTCGAGACACCTTCAAGGCTGGAGCTTCCTTGTATGGTGTAGCTGACTTAAACATGTTGAGGGCCGAAACTCACAAATTTGAATCTCAGTATATGGATAATCTTGTTGGTAGTGATCAGGATTTCTTTGATAGATCACCAATCAACTTTGCTGATAAATTTTCTTGCCCAATAATCTTATTCCAAGGATTGGAAGACAAGGTTGTACCCCCAGATCAAGCTCGTACAATTTACCATGCTCTGAAGGAAAAGGGCTTGCCAGTTGCTCTCGTGGAGTATCTATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

676

Amino Acids

75.28

Weight (kDa)

5.76

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 504 - 672 1.5e-40 Prolyl oligopeptidase family
BD-FAE PF20434 542 - 662 5.8e-08 BD-FAE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36210
fragaria_vesca FvH4_2g34950
malus_domestica MD08G1107000.v1.1 MD08G1107200.v1.1 MD15G1087700.v1.1 MD15G1087800.v1.1
prunus_persica Prupe.1G441500_v2.0.a1
pyrus_communis pycom08g08900 pycom08g08910 pycom15g08240
rosa_chinensis RchiOBHm_Chr6g0307051
rosa_laevigata RLG00000010738
rosa_multiflora Rmu_sc0005634.1_g000006
rosa_roxburghii Rroxscaffold_7G00161200
rosa_rugosa Rorug06G0355500
rosa_samantha Rh6AG467000 Rh6CG482500 Rh6DG468800
rosa_wichuraiana Rw6G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AarI CACCTGC 1 cut(s) 1703
AatII GACGTC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 1703
AccB1I GGYRCC 4 cut(s) 221, 242, 407, 753
AccB7I CCANNNNNTGG 2 cut(s) 521, 1933
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 962
AccII CGCG 3 cut(s) 374, 603, 712
AccIII TCCGGA 2 cut(s) 145, 299
AclWI GGATC 4 cut(s) 462, 713, 726, 845
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 8 cut(s) 616, 1001, 1033, 1043, 1283, 1497, 1820, 1901
AcuI CTGAAG 4 cut(s) 397, 1321, 1506, 2006
AcvI CACGTG 1 cut(s) 1493
AcyI GRCGYC 3 cut(s) 134, 222, 408
AfaI GTAC 4 cut(s) 398, 504, 1950, 1969
AfiI CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1933
AflIII ACRYGT 1 cut(s) 1797
AgeI ACCGGT 1 cut(s) 346
AhlI ACTAGT 1 cut(s) 1526
AjnI CCWGG 1 cut(s) 1375
AloI GAACNNNNNNTCC 2 cut(s) 294, 326
AluBI AGCT 9 cut(s) 173, 483, 1086, 1422, 1484, 1644, 1769, 1787, 1964
AluI AGCT 9 cut(s) 173, 483, 1086, 1422, 1484, 1644, 1769, 1787, 1964
Alw21I GWGCWC 1 cut(s) 103
Alw26I GTCTC 7 cut(s) 29, 220, 288, 325, 786, 826, 1743
AlwI GGATC 4 cut(s) 462, 713, 726, 845
Ama87I CYCGRG 1 cut(s) 278
Aor13HI TCCGGA 2 cut(s) 145, 299
AoxI GGCC 2 cut(s) 262, 1806
ApeKI GCWGC 4 cut(s) 170, 609, 1083, 1641
ApoI RAATTY 8 cut(s) 616, 1001, 1033, 1043, 1283, 1497, 1820, 1901
AseI ATTAAT 1 cut(s) 1352
AsiGI ACCGGT 1 cut(s) 346
AspLEI GCGC 2 cut(s) 224, 410
AspS9I GGNCC 4 cut(s) 527, 888, 1475, 1806
AsuHPI GGTGA 6 cut(s) 10, 193, 489, 1261, 1299, 1872
AsuII TTCGAA 1 cut(s) 379
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 3 cut(s) 527, 888, 1475
BaeI ACNNNNGTAYC 2 cut(s) 420, 453
BamHI GGATCC 1 cut(s) 718
BanI GGYRCC 4 cut(s) 221, 242, 407, 753
BanII GRGCYC 1 cut(s) 1139
BauI CACGAG 2 cut(s) 184, 2012
BbrPI CACGTG 1 cut(s) 1493
BbsI GAAGAC 4 cut(s) 345, 430, 1293, 1944
Bbv12I GWGCWC 1 cut(s) 103
BbvI GCAGC 4 cut(s) 182, 621, 1095, 1653
BccI CCATC 6 cut(s) 23, 542, 644, 765, 1417, 1673
BceAI ACGGC 4 cut(s) 95, 233, 277, 415
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 1 cut(s) 1377
BciVI GTATCC 1 cut(s) 1712
BclI TGATCA 2 cut(s) 1261, 1858
BcoDI GTCTC 7 cut(s) 29, 220, 288, 325, 786, 826, 1743
BcuI ACTAGT 1 cut(s) 1526
BfaI CTAG 3 cut(s) 1419, 1527, 1650
BfmI CTRYAG 2 cut(s) 1087, 1710
BfoI RGCGCY 2 cut(s) 225, 411
BfuAI ACCTGC 1 cut(s) 1703
BfuI GTATCC 1 cut(s) 1712
BisI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1734
BlsI GCNGC 8 cut(s) 172, 234, 242, 266, 611, 1085, 1643, 1735
Bme1390I CCNGG 1 cut(s) 1377
Bme18I GGWCC 3 cut(s) 527, 888, 1475
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 4 cut(s) 527, 888, 1475, 1806
BmiI GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
BmrFI CCNGG 1 cut(s) 1377
BmsI GCATC 4 cut(s) 78, 550, 985, 1226
BpiI GAAGAC 4 cut(s) 345, 430, 1293, 1944
BpmI CTGGAG 3 cut(s) 498, 1722, 1785
Bpu10I CCTNAGC 3 cut(s) 390, 1245, 1729
Bpu14I TTCGAA 1 cut(s) 379
BsaAI YACGTR 1 cut(s) 1493
BsaHI GRCGYC 3 cut(s) 134, 222, 408
BsaI GGTCTC 2 cut(s) 325, 786
BsaJI CCNNGG 4 cut(s) 259, 770, 1468, 1927
BsaWI WCCGGW 5 cut(s) 145, 299, 325, 334, 346
Bsc4I CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1933
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 6 cut(s) 21, 500, 530, 1576, 1705, 2003
BseAI TCCGGA 2 cut(s) 145, 299
BseBI CCWGG 1 cut(s) 1377
BseDI CCNNGG 4 cut(s) 259, 770, 1468, 1927
BseGI GGATG 7 cut(s) 695, 757, 1000, 1129, 1228, 1444, 1630
BseLI CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1933
BseMII CTCAG 4 cut(s) 97, 404, 798, 1844
BseNI ACTGG 6 cut(s) 21, 500, 530, 1576, 1705, 2003
BseX3I CGGCCG 1 cut(s) 262
BseXI GCAGC 4 cut(s) 182, 621, 1095, 1653
Bsh1236I CGCG 3 cut(s) 374, 603, 712
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 2 cut(s) 264, 1808
BshNI GGYRCC 4 cut(s) 221, 242, 407, 753
BshTI ACCGGT 1 cut(s) 346
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 103
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 6 cut(s) 146, 219, 300, 326, 335, 347
BslFI GGGAC 2 cut(s) 178, 671
BslI CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1933
BsmAI GTCTC 7 cut(s) 29, 220, 288, 325, 786, 826, 1743
BsmBI CGTCTC 1 cut(s) 220
BsmFI GGGAC 2 cut(s) 178, 671
BsmI GAATGC 1 cut(s) 1541
BsnI GGCC 2 cut(s) 264, 1808
Bso31I GGTCTC 2 cut(s) 325, 786
BsoBI CYCGRG 1 cut(s) 278
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 2 cut(s) 103, 1139
Bsp13I TCCGGA 2 cut(s) 145, 299
Bsp143I GATC 8 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957
Bsp19I CCATGG 2 cut(s) 770, 1468
Bsp68I TCGCGA 1 cut(s) 603
BspANI GGCC 2 cut(s) 264, 1808
BspCNI CTCAG 4 cut(s) 96, 403, 799, 1843
BspEI TCCGGA 2 cut(s) 145, 299
BspFNI CGCG 3 cut(s) 374, 603, 712
BspLI GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
BspMAI CTGCAG 1 cut(s) 1714
BspMI ACCTGC 1 cut(s) 1703
BspPI GGATC 4 cut(s) 462, 713, 726, 845
BspT104I TTCGAA 1 cut(s) 379
BspT107I GGYRCC 4 cut(s) 221, 242, 407, 753
BspTNI GGTCTC 2 cut(s) 325, 786
BsrBI CCGCTC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 6 cut(s) 21, 500, 530, 1576, 1705, 2003
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 4 cut(s) 259, 770, 1468, 1927
BssMI GATC 8 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957
BssNI GRCGYC 3 cut(s) 134, 222, 408
BssSI CACGAG 2 cut(s) 184, 2012
BssT1I CCWWGG 3 cut(s) 770, 1468, 1927
Bst2BI CACGAG 2 cut(s) 184, 2012
Bst2UI CCWGG 1 cut(s) 1377
Bst4CI ACNGT 4 cut(s) 575, 586, 640, 1562
BstACI GRCGYC 3 cut(s) 134, 222, 408
BstBAI YACGTR 1 cut(s) 1493
BstBI TTCGAA 1 cut(s) 379
BstC8I GCNNGC 2 cut(s) 1081, 2000
BstDEI CTNAG 8 cut(s) 83, 390, 807, 1245, 1434, 1508, 1729, 1830
BstDSI CCRYGG 3 cut(s) 259, 770, 1468
BstF5I GGATG 7 cut(s) 695, 757, 1000, 1129, 1228, 1444, 1630
BstFNI CGCG 3 cut(s) 374, 603, 712
BstH2I RGCGCY 2 cut(s) 225, 411
BstHHI GCGC 2 cut(s) 224, 410
BstKTI GATC 8 cut(s) 457, 721, 853, 1106, 1264, 1861, 1880, 1960
BstMAI GTCTC 7 cut(s) 29, 220, 288, 325, 786, 826, 1743
BstMBI GATC 8 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 3 cut(s) 414, 609, 1739
BstNI CCWGG 1 cut(s) 1377
BstNSI RCATGY 2 cut(s) 769, 1801
BstSCI CCNGG 1 cut(s) 1375
BstSFI CTRYAG 2 cut(s) 1087, 1710
BstUI CGCG 3 cut(s) 374, 603, 712
BstV1I GCAGC 4 cut(s) 182, 621, 1095, 1653
BstV2I GAAGAC 4 cut(s) 345, 430, 1293, 1944
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
BstZI CGGCCG 1 cut(s) 262
BsuI GTATCC 1 cut(s) 1712
BsuRI GGCC 2 cut(s) 264, 1808
BtgI CCRYGG 3 cut(s) 259, 770, 1468
BtgZI GCGATG 1 cut(s) 76
BtsCI GGATG 7 cut(s) 695, 757, 1000, 1129, 1228, 1444, 1630
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 3 cut(s) 64, 582, 1569
BtuMI TCGCGA 1 cut(s) 603
BveI ACCTGC 1 cut(s) 1703
Cac8I GCNNGC 2 cut(s) 1081, 2000
CfoI GCGC 2 cut(s) 224, 410
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 4 cut(s) 527, 888, 1475, 1806
CseI GACGC 1 cut(s) 701
Csp6I GTAC 4 cut(s) 397, 503, 1949, 1968
CspAI ACCGGT 1 cut(s) 346
CviAII CATG 8 cut(s) 613, 741, 766, 771, 1058, 1469, 1798, 1979
CviQI GTAC 4 cut(s) 397, 503, 1949, 1968
DdeI CTNAG 8 cut(s) 83, 390, 807, 1245, 1434, 1508, 1729, 1830
DinI GGCGCC 2 cut(s) 223, 409
DpnI GATC 8 cut(s) 456, 720, 852, 1105, 1263, 1860, 1879, 1959
DpnII GATC 8 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957
DraI TTTAAA 1 cut(s) 1503
EaeI YGGCCR 1 cut(s) 262
EagI CGGCCG 1 cut(s) 262
EciI GGCGGA 1 cut(s) 417
EclXI CGGCCG 1 cut(s) 262
Eco130I CCWWGG 3 cut(s) 770, 1468, 1927
Eco24I GRGCYC 1 cut(s) 1139
Eco31I GGTCTC 2 cut(s) 325, 786
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 3 cut(s) 527, 888, 1475
Eco52I CGGCCG 1 cut(s) 262
Eco57I CTGAAG 4 cut(s) 397, 1321, 1506, 2006
Eco72I CACGTG 1 cut(s) 1493
Eco88I CYCGRG 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 1475
EcoRI GAATTC 3 cut(s) 616, 1033, 1283
EcoRII CCWGG 1 cut(s) 1375
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 3 cut(s) 770, 1468, 1927
EcoT38I GRGCYC 1 cut(s) 1139
EgeI GGCGCC 2 cut(s) 223, 409
EheI GGCGCC 2 cut(s) 223, 409
ErhI CCWWGG 3 cut(s) 770, 1468, 1927
Esp3I CGTCTC 1 cut(s) 220
FaeI CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1801, 1982
FaqI GGGAC 2 cut(s) 178, 671
FatI CATG 8 cut(s) 612, 740, 765, 770, 1057, 1468, 1797, 1978
FauI CCCGC 1 cut(s) 717
FbaI TGATCA 2 cut(s) 1261, 1858
FblI GTMKAC 1 cut(s) 962
Fnu4HI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1734
FokI GGATG 7 cut(s) 702, 744, 1007, 1136, 1215, 1451, 1637
FriOI GRGCYC 1 cut(s) 1139
Fsp4HI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1734
FspBI CTAG 3 cut(s) 1419, 1527, 1650
GlaI GCGC 2 cut(s) 223, 409
GluI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1734
GsuI CTGGAG 3 cut(s) 498, 1722, 1785
HaeII RGCGCY 2 cut(s) 225, 411
HaeIII GGCC 2 cut(s) 264, 1808
HapII CCGG 6 cut(s) 146, 219, 300, 326, 335, 347
HgaI GACGC 1 cut(s) 701
HhaI GCGC 2 cut(s) 224, 410
Hin1I GRCGYC 3 cut(s) 134, 222, 408
Hin1II CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1801, 1982
Hin6I GCGC 2 cut(s) 222, 408
HinP1I GCGC 2 cut(s) 222, 408
HincII GTYRAC 1 cut(s) 1555
HindII GTYRAC 1 cut(s) 1555
HpaI GTTAAC 1 cut(s) 1555
HpaII CCGG 6 cut(s) 146, 219, 300, 326, 335, 347
HphI GGTGA 6 cut(s) 10, 193, 489, 1261, 1299, 1872
Hpy166II GTNNAC 4 cut(s) 77, 527, 963, 1555
Hpy188I TCNGA 7 cut(s) 86, 295, 673, 808, 850, 1222, 1986
Hpy8I GTNNAC 4 cut(s) 77, 527, 963, 1555
Hpy99I CGWCG 2 cut(s) 254, 295
HpyAV CCTTC 7 cut(s) 358, 421, 648, 715, 1205, 1765, 1981
HpyCH4III ACNGT 4 cut(s) 575, 586, 640, 1562
HpyCH4IV ACGT 3 cut(s) 134, 565, 1492
HpyCH4V TGCA 9 cut(s) 69, 563, 740, 857, 1083, 1394, 1641, 1712, 1742
HpyF10VI GCNNNNNNNGC 3 cut(s) 414, 609, 1739
HpyF3I CTNAG 8 cut(s) 83, 390, 807, 1245, 1434, 1508, 1729, 1830
HpySE526I ACGT 3 cut(s) 134, 565, 1492
Hsp92I GRCGYC 3 cut(s) 134, 222, 408
Hsp92II CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1801, 1982
HspAI GCGC 2 cut(s) 222, 408
KasI GGCGCC 2 cut(s) 221, 407
Kpn2I TCCGGA 2 cut(s) 145, 299
Ksp22I TGATCA 2 cut(s) 1261, 1858
KspAI GTTAAC 1 cut(s) 1555
Kzo9I GATC 8 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957
LmnI GCTCC 4 cut(s) 178, 488, 1214, 1766
Lsp1109I GCAGC 4 cut(s) 182, 621, 1095, 1653
LweI GCATC 4 cut(s) 78, 550, 985, 1226
MaeI CTAG 3 cut(s) 1419, 1527, 1650
MaeII ACGT 3 cut(s) 134, 565, 1492
MaeIII GTNAC 3 cut(s) 634, 692, 1249
MalI GATC 8 cut(s) 456, 720, 852, 1105, 1263, 1860, 1879, 1959
MbiI CCGCTC 1 cut(s) 308
MboI GATC 8 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957
MfeI CAATTG 3 cut(s) 630, 873, 1195
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 2 cut(s) 103, 1139
Mly113I GGCGCC 2 cut(s) 222, 408
MlyI GAGTC 5 cut(s) 81, 290, 840, 968, 1337
MmeI TCCRAC 1 cut(s) 273
MnlI CCTC 9 cut(s) 235, 278, 398, 582, 964, 1458, 1466, 1697, 1797
MroI TCCGGA 2 cut(s) 145, 299
MseI TTAA 9 cut(s) 479, 570, 861, 1152, 1319, 1352, 1502, 1554, 1793
MspA1I CMGCKG 2 cut(s) 1484, 1644
MspI CCGG 6 cut(s) 146, 219, 300, 326, 335, 347
MspR9I CCNGG 1 cut(s) 1377
MunI CAATTG 3 cut(s) 630, 873, 1195
Mva1269I GAATGC 1 cut(s) 1541
MvaI CCWGG 1 cut(s) 1377
MvnI CGCG 3 cut(s) 374, 603, 712
MwoI GCNNNNNNNGC 3 cut(s) 414, 609, 1739
NarI GGCGCC 2 cut(s) 222, 408
NcoI CCATGG 2 cut(s) 770, 1468
NdeII GATC 8 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957
NlaIII CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1801, 1982
NlaIV GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 2 cut(s) 692, 1249
NruI TCGCGA 1 cut(s) 603
NspI RCATGY 2 cut(s) 769, 1801
NspV TTCGAA 1 cut(s) 379
PaeR7I CTCGAG 1 cut(s) 278
PaqCI CACCTGC 1 cut(s) 1703
PciI ACATGT 1 cut(s) 1797
PcsI WCGNNNNNNNCGW 1 cut(s) 571
PctI GAATGC 1 cut(s) 1541
PfeI GAWTC 8 cut(s) 254, 296, 491, 668, 866, 940, 1664, 1826
PflMI CCANNNNNTGG 2 cut(s) 521, 1933
PinAI ACCGGT 1 cut(s) 346
PkrI GCNGC 8 cut(s) 172, 234, 242, 266, 611, 1085, 1643, 1735
PleI GAGTC 5 cut(s) 81, 289, 839, 967, 1336
PluTI GGCGCC 2 cut(s) 225, 411
PmaCI CACGTG 1 cut(s) 1493
PmlI CACGTG 1 cut(s) 1493
PpsI GAGTC 5 cut(s) 81, 289, 839, 967, 1336
Ppu21I YACGTR 1 cut(s) 1493
PpuMI RGGWCCY 1 cut(s) 1475
PscI ACATGT 1 cut(s) 1797
PshBI ATTAAT 1 cut(s) 1352
PsiI TTATAA 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 1475
Psp6I CCWGG 1 cut(s) 1375
PspCI CACGTG 1 cut(s) 1493
PspGI CCWGG 1 cut(s) 1375
PspN4I GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
PspPI GGNCC 4 cut(s) 527, 888, 1475, 1806
PspPPI RGGWCCY 1 cut(s) 1475
PspXI VCTCGAGB 1 cut(s) 278
PstI CTGCAG 1 cut(s) 1714
PsuI RGATCY 1 cut(s) 718
PvuII CAGCTG 2 cut(s) 1484, 1644
RruI TCGCGA 1 cut(s) 603
RsaI GTAC 4 cut(s) 398, 504, 1950, 1969
RsaNI GTAC 4 cut(s) 397, 503, 1949, 1968
SaqAI TTAA 9 cut(s) 479, 570, 861, 1152, 1319, 1352, 1502, 1554, 1793
SatI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1734
Sau3AI GATC 8 cut(s) 454, 718, 850, 1103, 1261, 1858, 1877, 1957
Sau96I GGNCC 4 cut(s) 527, 888, 1475, 1806
SchI GAGTC 5 cut(s) 81, 290, 840, 968, 1337
ScrFI CCNGG 1 cut(s) 1377
SduI GDGCHC 2 cut(s) 103, 1139
SfaNI GCATC 4 cut(s) 78, 550, 985, 1226
SfcI CTRYAG 2 cut(s) 1087, 1710
SfoI GGCGCC 2 cut(s) 223, 409
Sfr274I CTCGAG 1 cut(s) 278
SfuI TTCGAA 1 cut(s) 379
SinI GGWCC 3 cut(s) 527, 888, 1475
SlaI CTCGAG 1 cut(s) 278
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SpeI ACTAGT 1 cut(s) 1526
SspDI GGCGCC 2 cut(s) 221, 407
SspI AATATT 2 cut(s) 1156, 1180
SspMI CTAG 3 cut(s) 1419, 1527, 1650
StyD4I CCNGG 1 cut(s) 1375
StyI CCWWGG 3 cut(s) 770, 1468, 1927
TaaI ACNGT 4 cut(s) 575, 586, 640, 1562
TaiI ACGT 3 cut(s) 137, 568, 1495
TaqI TCGA 4 cut(s) 252, 279, 379, 1747
TauI GCSGC 4 cut(s) 235, 243, 267, 1736
TfiI GAWTC 8 cut(s) 254, 296, 491, 668, 866, 940, 1664, 1826
Tru1I TTAA 9 cut(s) 479, 570, 861, 1152, 1319, 1352, 1502, 1554, 1793
Tru9I TTAA 9 cut(s) 479, 570, 861, 1152, 1319, 1352, 1502, 1554, 1793
TscAI CASTG 3 cut(s) 71, 589, 1576
TseFI GTSAC 2 cut(s) 692, 1249
TseI GCWGC 4 cut(s) 170, 609, 1083, 1641
Tsp45I GTSAC 2 cut(s) 692, 1249
TspDTI ATGAA 6 cut(s) 629, 901, 1056, 1074, 1217, 1455
TspGWI ACGGA 2 cut(s) 279, 1181
TspRI CASTG 3 cut(s) 71, 589, 1576
Van91I CCANNNNNTGG 2 cut(s) 521, 1933
VpaK11BI GGWCC 3 cut(s) 527, 888, 1475
VspI ATTAAT 1 cut(s) 1352
XapI RAATTY 8 cut(s) 616, 1001, 1033, 1043, 1283, 1497, 1820, 1901
XceI RCATGY 2 cut(s) 769, 1801
XhoI CTCGAG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 962
XspI CTAG 3 cut(s) 1419, 1527, 1650
ZraI GACGTC 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.