MD15G1087800.v1.1

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012367
Physical Location & Seq
Reverse (-)
6109483 .. 6115628
6146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1087800.v1.1.491

Sequence Viewer

Length: 2160 bp
ATGGCTGCTTTCACTCTCACTCTCACTCTCACTCTCACTCGCTTATCTTCGTCTTCCTTCAATCGAATTAGTCAACGATTGAGCTCCAGAGTACAGACGAACAGCCACCGTCTCCACCAAATGGCTACTTCTTCCTCCCCCGAAGTCAAGCAATCGGAGAAAATCGTAGCTCCTTACGGGTCCTGGAAATCCCCAATCTCCGCCGATGTCGTCTCTGGCGCCTCCAAGAGACTCGGCGGCACGGCCGTCGATTCCCTCGGCCGCCTCATCTGGCTCGAGTCCCGCCCATCTGAATCAGGACGGGCGGTTCTGGTGAGGGAACCGGAGAGGCCGGAGGACGAACCGGTTGATATTACGCCGAAGGACTATGCGGTTCGGACGGTGGCGCAGGAGTACGGCGGCGGTGCATTCAGCGTTTCAGGGGATACAATCGTTTTCTGTAATTACAAGGATCAGCGTCTTTACAAGCAGTCCCTAAGTTCGAAAGATTCTGCTCCTACACCACTCACTCCGGATTATGGCGGGCCGGTAATTAGTTATGCTGATGGCGTGTTTGATGCACGTTTTAATCGTTTTGTCACTGTACGGGAAGATCGTCGTGAAAGCAGCATTAATTCGATAACAACAATTGTAACAGTAGGGCTTGATGGCAAGGAAATTCAGGAACCAGAAGTACTAGTTGGTGGGACGGATTTCTATGCCTTCCCGCGTTTTGATCCGAAAGGTGAACGTCTGGCATGGATTGAATGGTGCCATCCTAATATGCCATGGGATAGGTCTCAACTTTGGGTTGGGTATATATCCGAGAAGGGAGAAGTCTATAAACGAATTTGTGTTGCTGGCTCTGATCCTTCCATTAAGGAGTCCCCAATTGAACCCAAGTGGTCCTCTAAAGGGGAACTATTTTTTATTACTGACAGAAAAGGTGGGTACTGGAATCTTTATAAATGGGTTGAGTCTAGTAATGAGGTCGTATCAGTTTATTCCTTGGATGCCGAGTTTTCCAAGCCATTATGGGTTTTTGGCATGAATTCTTATGAATTTATCGAGAGCCATGAAAGTAAAAACATAATTGCTTGCAGCTACAGGCAGAAGGGGAGGTCATATCTTGGAATCCTGGATGATGCTCAGGGCTCACTGTCTCTACTTAATACTCCTTTTACAGATATTGATAATATTACCTTGGGGATCAATTGCCTGTACATTGAAGGAGCATCAGAAGTTCAACCTTTGGCTGTTGCTAAGGTGACTTTAGATGATCATAGAACGAAAGCAGTTGACTCCAAGATTATTTGGTCTTCCTCACCTGATTGCTTAAAATATCAGTCCTACTTCAGCCTACCCGAATTAATTGAGTTCCCAACAGAAGTTCCTGGTCAAACTGCCTTCGCATACTTTTACCCACCAACAAATCCCAATTACCAAGCTCCTCAGGGTGAAAAGCCTCCACTGTTATTGAAAAGCCACGGAGGGCCCACAGCCGAATCACATGGAATCTTAAATTTGAGTATTCAGTATTGGACTAGTCGTGGTTGGGCATTTGTCGATGTTAACTATGGTGGAAGCACTGGTTATGGCAGAGAATATCGGGAACGACTCTTGAACAAATGGGGAATTGTGGATGTAAATGATTGTTGCAGCTGTGCTAGATATCTGGTGGATTCCGGAAAGGTAGATGGGGAACGACTTTGTATTACTGGGGGATCTGCAGGTGGCTATACAACCCTAGCTGCTCTTGCTTTTAGAGATACCTTCAAGGCAGGATCTTCCTTGTATGGTGTAGCCGACTTAAACATGTTGAGGGCAGAAACTCACAAATTTGAGTCTCATTATATTGATAATCTTGTTGGTAGTGAAAAGGATTATCATGATAGGTCACCCATCAACTTTGCTGATAAGTTTTCTTGCCCGATAATCTTATTCCAAGGGTTGGAAGACAAGGTTGTAGCCCCAGATCAAGCTCGTAAAATTTATCAGGCTTTGAAGGAAAAGGGTCTGCCTGTTGCTCATGTGGAGTATGAAGGAGAACAACATGGCTTCCGCAAGGCTGAGAACATTAAGTTCACGCTTGAACAGCAAATGGTTTTCTTTGCTCGTTTAGTTGGAAACTTCAAGGTTGCGGATGAGATTAATCCTATTAAAATTGACAACTTCGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

720

Amino Acids

80.34

Weight (kDa)

5.59

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 503 - 702 1.3e-46 Prolyl oligopeptidase family
BD-FAE PF20434 541 - 661 9.2e-08 BD-FAE
DLH PF01738 550 - 686 3.1e-07 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36210
fragaria_vesca FvH4_2g34950
malus_domestica MD08G1107000.v1.1 MD08G1107200.v1.1 MD15G1087700.v1.1 MD15G1087800.v1.1
prunus_persica Prupe.1G441500_v2.0.a1
pyrus_communis pycom08g08900 pycom08g08910 pycom15g08240
rosa_chinensis RchiOBHm_Chr6g0307051
rosa_laevigata RLG00000010738
rosa_multiflora Rmu_sc0005634.1_g000006
rosa_roxburghii Rroxscaffold_7G00161200
rosa_rugosa Rorug06G0355500
rosa_samantha Rh6AG467000 Rh6CG482500 Rh6DG468800
rosa_wichuraiana Rw6G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 945
AarI CACCTGC 1 cut(s) 1700
Acc36I ACCTGC 1 cut(s) 1700
AccB1I GGYRCC 2 cut(s) 218, 750
AccB7I CCANNNNNTGG 2 cut(s) 121, 1930
AccII CGCG 1 cut(s) 709
AccIII TCCGGA 2 cut(s) 511, 1664
AclWI GGATC 6 cut(s) 459, 710, 842, 1196, 1711, 1771
AcoI YGGCCR 2 cut(s) 243, 259
AcsI RAATTY 7 cut(s) 657, 828, 1030, 1040, 1501, 1817, 1968
AcuI CTGAAG 1 cut(s) 1318
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 6 cut(s) 93, 395, 585, 675, 932, 1202
AfiI CCNNNNNNNGG 4 cut(s) 121, 518, 894, 1930
AflIII ACRYGT 1 cut(s) 1794
AgeI ACCGGT 1 cut(s) 343
AhlI ACTAGT 2 cut(s) 676, 1523
AjnI CCWGG 3 cut(s) 182, 1116, 1372
AjuI GAANNNNNNNTTGG 2 cut(s) 663, 695
AloI GAACNNNNNNTCC 2 cut(s) 291, 323
AluBI AGCT 7 cut(s) 84, 170, 1083, 1427, 1641, 1730, 1961
AluI AGCT 7 cut(s) 84, 170, 1083, 1427, 1641, 1730, 1961
Alw21I GWGCWC 1 cut(s) 86
Alw26I GTCTC 6 cut(s) 116, 217, 223, 783, 1146, 1830
AlwI GGATC 6 cut(s) 459, 710, 842, 1196, 1711, 1771
Ama87I CYCGRG 1 cut(s) 275
Aor13HI TCCGGA 2 cut(s) 511, 1664
AoxI GGCC 5 cut(s) 243, 259, 329, 524, 1472
ApaI GGGCCC 1 cut(s) 1476
ApeKI GCWGC 5 cut(s) 5, 606, 1080, 1638, 1730
ApoI RAATTY 7 cut(s) 657, 828, 1030, 1040, 1501, 1817, 1968
AseI ATTAAT 3 cut(s) 612, 1349, 2130
AsiGI ACCGGT 1 cut(s) 343
AspLEI GCGC 2 cut(s) 221, 388
AspS9I GGNCC 5 cut(s) 180, 524, 885, 1472, 1473
AsuHPI GGTGA 6 cut(s) 325, 737, 1258, 1296, 1448, 1869
AsuII TTCGAA 1 cut(s) 482
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 2 cut(s) 180, 885
AxyI CCTNAGG 1 cut(s) 1431
BaeGI GKGCMC 1 cut(s) 1476
BaeI ACNNNNGTAYC 2 cut(s) 417, 450
BanI GGYRCC 2 cut(s) 218, 750
BanII GRGCYC 3 cut(s) 86, 1136, 1476
BbsI GAAGAC 3 cut(s) 45, 1290, 1941
Bbv12I GWGCWC 1 cut(s) 86
BbvI GCAGC 4 cut(s) 618, 1092, 1650, 1717
BccI CCATC 6 cut(s) 295, 539, 641, 762, 1670, 1889
BceAI ACGGC 3 cut(s) 230, 258, 412
BcgI CGANNNNNNTGC 2 cut(s) 229, 263
BciT130I CCWGG 3 cut(s) 184, 1118, 1374
BciVI GTATCC 1 cut(s) 418
BclI TGATCA 1 cut(s) 1258
BcoDI GTCTC 6 cut(s) 116, 217, 223, 783, 1146, 1830
BcuI ACTAGT 2 cut(s) 676, 1523
BfaI CTAG 6 cut(s) 677, 960, 1524, 1647, 1727, 2158
BfmI CTRYAG 2 cut(s) 1084, 1707
BfoI RGCGCY 1 cut(s) 222
BfuAI ACCTGC 1 cut(s) 1700
BfuI GTATCC 1 cut(s) 418
BisI GCNGC 8 cut(s) 6, 238, 262, 400, 607, 1081, 1639, 1731
BlsI GCNGC 8 cut(s) 7, 239, 263, 401, 608, 1082, 1640, 1732
BmcAI AGTACT 1 cut(s) 675
Bme1390I CCNGG 3 cut(s) 184, 1118, 1374
Bme18I GGWCC 2 cut(s) 180, 885
BmeT110I CYCGRG 1 cut(s) 275
BmgT120I GGNCC 5 cut(s) 180, 524, 885, 1472, 1473
BmiI GGNNCC 6 cut(s) 181, 220, 321, 666, 752, 1474
BmrFI CCNGG 3 cut(s) 184, 1118, 1374
BmrI ACTGGG 1 cut(s) 1707
BmsI GCATC 4 cut(s) 547, 982, 1114, 1223
BmuI ACTGGG 1 cut(s) 1707
BpiI GAAGAC 3 cut(s) 45, 1290, 1941
BpmI CTGGAG 1 cut(s) 70
Bpu10I CCTNAGC 2 cut(s) 1128, 1242
Bpu14I TTCGAA 1 cut(s) 482
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 783
BsaJI CCNNGG 6 cut(s) 256, 767, 987, 1182, 1465, 1924
BsaWI WCCGGW 4 cut(s) 322, 343, 511, 1664
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 518, 894, 1930
Bse118I RCCGGY 2 cut(s) 343, 526
Bse1I ACTGG 3 cut(s) 938, 1573, 1702
Bse21I CCTNAGG 1 cut(s) 1431
BseAI TCCGGA 2 cut(s) 511, 1664
BseBI CCWGG 3 cut(s) 184, 1118, 1374
BseDI CCNNGG 6 cut(s) 256, 767, 987, 1182, 1465, 1924
BseGI GGATG 5 cut(s) 754, 997, 1126, 1627, 2128
BseLI CCNNNNNNNGG 4 cut(s) 121, 518, 894, 1930
BseMII CTCAG 3 cut(s) 1142, 1445, 2040
BseNI ACTGG 3 cut(s) 938, 1573, 1702
BseRI GAGGAG 1 cut(s) 1419
BseSI GKGCMC 1 cut(s) 1476
BseX3I CGGCCG 2 cut(s) 243, 259
BseXI GCAGC 4 cut(s) 618, 1092, 1650, 1717
Bsh1236I CGCG 1 cut(s) 709
Bsh1285I CGRYCG 2 cut(s) 246, 262
BshFI GGCC 5 cut(s) 245, 261, 331, 526, 1474
BshNI GGYRCC 2 cut(s) 218, 750
BshTI ACCGGT 1 cut(s) 343
BsiEI CGRYCG 2 cut(s) 246, 262
BsiHKAI GWGCWC 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 6 cut(s) 323, 332, 344, 512, 527, 1665
BslFI GGGAC 4 cut(s) 265, 457, 700, 850
BslI CCNNNNNNNGG 4 cut(s) 121, 518, 894, 1930
BsmAI GTCTC 6 cut(s) 116, 217, 223, 783, 1146, 1830
BsmBI CGTCTC 2 cut(s) 116, 217
BsmFI GGGAC 4 cut(s) 265, 457, 700, 850
BsmI GAATGC 1 cut(s) 407
BsnI GGCC 5 cut(s) 245, 261, 331, 526, 1474
Bso31I GGTCTC 1 cut(s) 783
BsoBI CYCGRG 1 cut(s) 275
Bsp119I TTCGAA 1 cut(s) 482
Bsp120I GGGCCC 1 cut(s) 1472
Bsp1286I GDGCHC 3 cut(s) 86, 1136, 1476
Bsp13I TCCGGA 2 cut(s) 511, 1664
Bsp1407I TGTACA 1 cut(s) 1200
Bsp143I GATC 9 cut(s) 451, 592, 715, 847, 1188, 1258, 1703, 1763, 1954
Bsp19I CCATGG 1 cut(s) 767
BspANI GGCC 5 cut(s) 245, 261, 331, 526, 1474
BspCNI CTCAG 3 cut(s) 1141, 1444, 2041
BspEI TCCGGA 2 cut(s) 511, 1664
BspFNI CGCG 1 cut(s) 709
BspHI TCATGA 1 cut(s) 1867
BspLI GGNNCC 6 cut(s) 181, 220, 321, 666, 752, 1474
BspMAI CTGCAG 1 cut(s) 1711
BspMI ACCTGC 1 cut(s) 1700
BspPI GGATC 6 cut(s) 459, 710, 842, 1196, 1711, 1771
BspT104I TTCGAA 1 cut(s) 482
BspT107I GGYRCC 2 cut(s) 218, 750
BspTNI GGTCTC 1 cut(s) 783
BsrFI RCCGGY 2 cut(s) 343, 526
BsrGI TGTACA 1 cut(s) 1200
BsrI ACTGG 3 cut(s) 938, 1573, 1702
BssAI RCCGGY 2 cut(s) 343, 526
BssECI CCNNGG 6 cut(s) 256, 767, 987, 1182, 1465, 1924
BssMI GATC 9 cut(s) 451, 592, 715, 847, 1188, 1258, 1703, 1763, 1954
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 4 cut(s) 767, 987, 1182, 1924
Bst2UI CCWGG 3 cut(s) 184, 1118, 1374
Bst4CI ACNGT 6 cut(s) 110, 382, 583, 637, 1140, 1452
BstACI GRCGYC 1 cut(s) 219
BstAUI TGTACA 1 cut(s) 1200
BstBI TTCGAA 1 cut(s) 482
BstC8I GCNNGC 3 cut(s) 524, 841, 1078
BstDEI CTNAG 5 cut(s) 476, 1128, 1242, 1431, 2049
BstDSI CCRYGG 2 cut(s) 767, 1465
BstEII GGTNACC 1 cut(s) 1875
BstF5I GGATG 5 cut(s) 754, 997, 1126, 1627, 2128
BstFNI CGCG 1 cut(s) 709
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 2 cut(s) 221, 388
BstKTI GATC 9 cut(s) 454, 595, 718, 850, 1191, 1261, 1706, 1766, 1957
BstMAI GTCTC 6 cut(s) 116, 217, 223, 783, 1146, 1830
BstMBI GATC 9 cut(s) 451, 592, 715, 847, 1188, 1258, 1703, 1763, 1954
BstMCI CGRYCG 2 cut(s) 246, 262
BstMWI GCNNNNNNNGC 2 cut(s) 1736, 2074
BstNI CCWGG 3 cut(s) 184, 1118, 1374
BstNSI RCATGY 1 cut(s) 1798
BstPI GGTNACC 1 cut(s) 1875
BstSCI CCNGG 3 cut(s) 182, 1116, 1372
BstSFI CTRYAG 2 cut(s) 1084, 1707
BstSLI GKGCMC 1 cut(s) 1476
BstUI CGCG 1 cut(s) 709
BstV1I GCAGC 4 cut(s) 618, 1092, 1650, 1717
BstV2I GAAGAC 3 cut(s) 45, 1290, 1941
BstX2I RGATCY 2 cut(s) 1703, 1763
BstYI RGATCY 2 cut(s) 1703, 1763
BstZI CGGCCG 2 cut(s) 243, 259
Bsu36I CCTNAGG 1 cut(s) 1431
BsuI GTATCC 1 cut(s) 418
BsuRI GGCC 5 cut(s) 245, 261, 331, 526, 1474
BtgI CCRYGG 2 cut(s) 767, 1465
BtsCI GGATG 5 cut(s) 754, 997, 1126, 1627, 2128
BtsIMutI CAGTG 4 cut(s) 579, 1136, 1448, 1566
BveI ACCTGC 1 cut(s) 1700
Cac8I GCNNGC 3 cut(s) 524, 841, 1078
CciI TCATGA 1 cut(s) 1867
CfoI GCGC 2 cut(s) 221, 388
Cfr10I RCCGGY 2 cut(s) 343, 526
Cfr13I GGNCC 5 cut(s) 180, 524, 885, 1472, 1473
CseI GACGC 1 cut(s) 446
Csp6I GTAC 6 cut(s) 92, 394, 584, 674, 931, 1201
CspAI ACCGGT 1 cut(s) 343
CspCI CAANNNNNGTGG 2 cut(s) 1437, 1472
CviAII CATG 9 cut(s) 738, 768, 1027, 1055, 1490, 1795, 1868, 2009, 2033
CviQI GTAC 6 cut(s) 92, 394, 584, 674, 931, 1201
DdeI CTNAG 5 cut(s) 476, 1128, 1242, 1431, 2049
DinI GGCGCC 1 cut(s) 220
DpnI GATC 9 cut(s) 453, 594, 717, 849, 1190, 1260, 1705, 1765, 1956
DpnII GATC 9 cut(s) 451, 592, 715, 847, 1188, 1258, 1703, 1763, 1954
EaeI YGGCCR 2 cut(s) 243, 259
EagI CGGCCG 2 cut(s) 243, 259
EciI GGCGGA 1 cut(s) 190
Ecl136II GAGCTC 1 cut(s) 84
EclXI CGGCCG 2 cut(s) 243, 259
Eco130I CCWWGG 4 cut(s) 767, 987, 1182, 1924
Eco24I GRGCYC 3 cut(s) 86, 1136, 1476
Eco31I GGTCTC 1 cut(s) 783
Eco32I GATATC 1 cut(s) 1652
Eco47I GGWCC 2 cut(s) 180, 885
Eco52I CGGCCG 2 cut(s) 243, 259
Eco53kI GAGCTC 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 1318
Eco81I CCTNAGG 1 cut(s) 1431
Eco88I CYCGRG 1 cut(s) 275
Eco91I GGTNACC 1 cut(s) 1875
EcoICRI GAGCTC 1 cut(s) 84
EcoO109I RGGNCCY 2 cut(s) 180, 1472
EcoO65I GGTNACC 1 cut(s) 1875
EcoRI GAATTC 1 cut(s) 1030
EcoRII CCWGG 3 cut(s) 182, 1116, 1372
EcoRV GATATC 1 cut(s) 1652
EcoT14I CCWWGG 4 cut(s) 767, 987, 1182, 1924
EcoT38I GRGCYC 3 cut(s) 86, 1136, 1476
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
ErhI CCWWGG 4 cut(s) 767, 987, 1182, 1924
Esp3I CGTCTC 2 cut(s) 116, 217
FaeI CATG 9 cut(s) 741, 771, 1030, 1058, 1493, 1798, 1871, 2012, 2036
FaqI GGGAC 4 cut(s) 265, 457, 700, 850
FatI CATG 9 cut(s) 737, 767, 1026, 1054, 1489, 1794, 1867, 2008, 2032
FauI CCCGC 3 cut(s) 290, 515, 714
FbaI TGATCA 1 cut(s) 1258
Fnu4HI GCNGC 8 cut(s) 6, 238, 262, 400, 607, 1081, 1639, 1731
FokI GGATG 5 cut(s) 741, 1004, 1133, 1634, 2135
FriOI GRGCYC 3 cut(s) 86, 1136, 1476
Fsp4HI GCNGC 8 cut(s) 6, 238, 262, 400, 607, 1081, 1639, 1731
FspBI CTAG 6 cut(s) 677, 960, 1524, 1647, 1727, 2158
GlaI GCGC 2 cut(s) 220, 387
GluI GCNGC 8 cut(s) 6, 238, 262, 400, 607, 1081, 1639, 1731
GsuI CTGGAG 1 cut(s) 70
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 5 cut(s) 245, 261, 331, 526, 1474
HapII CCGG 6 cut(s) 323, 332, 344, 512, 527, 1665
HgaI GACGC 1 cut(s) 446
HhaI GCGC 2 cut(s) 221, 388
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 9 cut(s) 741, 771, 1030, 1058, 1493, 1798, 1871, 2012, 2036
Hin6I GCGC 2 cut(s) 219, 386
HinP1I GCGC 2 cut(s) 219, 386
HincII GTYRAC 3 cut(s) 74, 1279, 1552
HindII GTYRAC 3 cut(s) 74, 1279, 1552
HpaI GTTAAC 1 cut(s) 1552
HpaII CCGG 6 cut(s) 323, 332, 344, 512, 527, 1665
HphI GGTGA 6 cut(s) 325, 737, 1258, 1296, 1448, 1869
Hpy166II GTNNAC 5 cut(s) 74, 728, 1279, 1552, 2064
Hpy188I TCNGA 7 cut(s) 157, 292, 378, 720, 805, 847, 1219
Hpy8I GTNNAC 5 cut(s) 74, 728, 1279, 1552, 2064
Hpy99I CGWCG 2 cut(s) 251, 600
HpyCH4III ACNGT 6 cut(s) 110, 382, 583, 637, 1140, 1452
HpyCH4IV ACGT 2 cut(s) 562, 730
HpyCH4V TGCA 5 cut(s) 407, 560, 1080, 1638, 1709
HpyF10VI GCNNNNNNNGC 2 cut(s) 1736, 2074
HpyF3I CTNAG 5 cut(s) 476, 1128, 1242, 1431, 2049
HpySE526I ACGT 2 cut(s) 562, 730
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 9 cut(s) 741, 771, 1030, 1058, 1493, 1798, 1871, 2012, 2036
HspAI GCGC 2 cut(s) 219, 386
KasI GGCGCC 1 cut(s) 218
Kpn2I TCCGGA 2 cut(s) 511, 1664
Ksp22I TGATCA 1 cut(s) 1258
KspAI GTTAAC 1 cut(s) 1552
Kzo9I GATC 9 cut(s) 451, 592, 715, 847, 1188, 1258, 1703, 1763, 1954
LmnI GCTCC 5 cut(s) 89, 175, 499, 1211, 1432
Lsp1109I GCAGC 4 cut(s) 618, 1092, 1650, 1717
LweI GCATC 4 cut(s) 547, 982, 1114, 1223
MaeI CTAG 6 cut(s) 677, 960, 1524, 1647, 1727, 2158
MaeII ACGT 2 cut(s) 562, 730
MaeIII GTNAC 4 cut(s) 577, 631, 1246, 1875
MalI GATC 9 cut(s) 453, 594, 717, 849, 1190, 1260, 1705, 1765, 1956
MboI GATC 9 cut(s) 451, 592, 715, 847, 1188, 1258, 1703, 1763, 1954
MboII GAAGA 7 cut(s) 39, 45, 123, 602, 1290, 1758, 1946
MfeI CAATTG 3 cut(s) 627, 870, 1192
MflI RGATCY 2 cut(s) 1703, 1763
MhlI GDGCHC 3 cut(s) 86, 1136, 1476
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 7 cut(s) 225, 287, 872, 965, 1274, 1590, 1832
MmeI TCCRAC 2 cut(s) 1911, 2083
MroI TCCGGA 2 cut(s) 511, 1664
MspA1I CMGCKG 1 cut(s) 1641
MspI CCGG 6 cut(s) 323, 332, 344, 512, 527, 1665
MspR9I CCNGG 3 cut(s) 184, 1118, 1374
MunI CAATTG 3 cut(s) 627, 870, 1192
Mva1269I GAATGC 1 cut(s) 407
MvaI CCWGG 3 cut(s) 184, 1118, 1374
MvnI CGCG 1 cut(s) 709
MwoI GCNNNNNNNGC 2 cut(s) 1736, 2074
NarI GGCGCC 1 cut(s) 219
NcoI CCATGG 1 cut(s) 767
NdeII GATC 9 cut(s) 451, 592, 715, 847, 1188, 1258, 1703, 1763, 1954
NlaIII CATG 9 cut(s) 741, 771, 1030, 1058, 1493, 1798, 1871, 2012, 2036
NlaIV GGNNCC 6 cut(s) 181, 220, 321, 666, 752, 1474
NmeAIII GCCGAG 3 cut(s) 213, 237, 1021
NmuCI GTSAC 3 cut(s) 577, 1246, 1875
NspI RCATGY 1 cut(s) 1798
NspV TTCGAA 1 cut(s) 482
PaeR7I CTCGAG 1 cut(s) 275
PagI TCATGA 1 cut(s) 1867
PaqCI CACCTGC 1 cut(s) 1700
PciI ACATGT 1 cut(s) 1794
PcsI WCGNNNNNNNCGW 2 cut(s) 568, 592
PctI GAATGC 1 cut(s) 407
PfeI GAWTC 8 cut(s) 251, 293, 488, 937, 1113, 1484, 1494, 1661
PflMI CCANNNNNTGG 2 cut(s) 121, 1930
PfoI TCCNGGA 2 cut(s) 182, 1116
PinAI ACCGGT 1 cut(s) 343
PkrI GCNGC 8 cut(s) 7, 239, 263, 401, 608, 1082, 1640, 1732
PleI GAGTC 7 cut(s) 225, 286, 871, 964, 1274, 1590, 1831
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 7 cut(s) 225, 286, 871, 964, 1274, 1590, 1831
PpuMI RGGWCCY 1 cut(s) 180
PscI ACATGT 1 cut(s) 1794
PshBI ATTAAT 3 cut(s) 612, 1349, 2130
PsiI TTATAA 1 cut(s) 945
Psp124BI GAGCTC 1 cut(s) 86
Psp5II RGGWCCY 1 cut(s) 180
Psp6I CCWGG 3 cut(s) 182, 1116, 1372
PspEI GGTNACC 1 cut(s) 1875
PspGI CCWGG 3 cut(s) 182, 1116, 1372
PspN4I GGNNCC 6 cut(s) 181, 220, 321, 666, 752, 1474
PspOMI GGGCCC 1 cut(s) 1472
PspPI GGNCC 5 cut(s) 180, 524, 885, 1472, 1473
PspPPI RGGWCCY 1 cut(s) 180
PspXI VCTCGAGB 1 cut(s) 275
PsrI GAACNNNNNNTAC 2 cut(s) 657, 689
PstI CTGCAG 1 cut(s) 1711
PsuI RGATCY 2 cut(s) 1703, 1763
PvuII CAGCTG 1 cut(s) 1641
RsaI GTAC 6 cut(s) 93, 395, 585, 675, 932, 1202
RsaNI GTAC 6 cut(s) 92, 394, 584, 674, 931, 1201
SacI GAGCTC 1 cut(s) 86
SatI GCNGC 8 cut(s) 6, 238, 262, 400, 607, 1081, 1639, 1731
Sau3AI GATC 9 cut(s) 451, 592, 715, 847, 1188, 1258, 1703, 1763, 1954
Sau96I GGNCC 5 cut(s) 180, 524, 885, 1472, 1473
ScaI AGTACT 1 cut(s) 675
SchI GAGTC 7 cut(s) 225, 287, 872, 965, 1274, 1590, 1832
ScrFI CCNGG 3 cut(s) 184, 1118, 1374
SduI GDGCHC 3 cut(s) 86, 1136, 1476
SfaNI GCATC 4 cut(s) 547, 982, 1114, 1223
SfcI CTRYAG 2 cut(s) 1084, 1707
SfoI GGCGCC 1 cut(s) 220
Sfr274I CTCGAG 1 cut(s) 275
SfuI TTCGAA 1 cut(s) 482
SinI GGWCC 2 cut(s) 180, 885
SlaI CTCGAG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SpeI ACTAGT 2 cut(s) 676, 1523
SspDI GGCGCC 1 cut(s) 218
SspI AATATT 1 cut(s) 1177
SspMI CTAG 6 cut(s) 677, 960, 1524, 1647, 1727, 2158
SstI GAGCTC 1 cut(s) 86
StyD4I CCNGG 3 cut(s) 182, 1116, 1372
StyI CCWWGG 4 cut(s) 767, 987, 1182, 1924
TaaI ACNGT 6 cut(s) 110, 382, 583, 637, 1140, 1452
TaiI ACGT 2 cut(s) 565, 733
TaqI TCGA 8 cut(s) 64, 249, 276, 482, 617, 1047, 1545, 2154
TatI WGTACW 3 cut(s) 91, 673, 1200
TauI GCSGC 3 cut(s) 240, 264, 402
TfiI GAWTC 8 cut(s) 251, 293, 488, 937, 1113, 1484, 1494, 1661
TscAI CASTG 4 cut(s) 586, 1143, 1455, 1573
TseFI GTSAC 3 cut(s) 577, 1246, 1875
TseI GCWGC 5 cut(s) 5, 606, 1080, 1638, 1730
Tsp45I GTSAC 3 cut(s) 577, 1246, 1875
TspDTI ATGAA 4 cut(s) 1043, 1053, 1071, 2034
TspGWI ACGGA 2 cut(s) 704, 1482
TspRI CASTG 4 cut(s) 586, 1143, 1455, 1573
Van91I CCANNNNNTGG 2 cut(s) 121, 1930
VpaK11BI GGWCC 2 cut(s) 180, 885
VspI ATTAAT 3 cut(s) 612, 1349, 2130
XapI RAATTY 7 cut(s) 657, 828, 1030, 1040, 1501, 1817, 1968
XceI RCATGY 1 cut(s) 1798
XhoI CTCGAG 1 cut(s) 275
XspI CTAG 6 cut(s) 677, 960, 1524, 1647, 1727, 2158
ZrmI AGTACT 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.