Rh6AG467000

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0012367
Physical Location & Seq
Forward (+)
64940477 .. 64945914
5438 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG467000.1

Sequence Viewer

Length: 2148 bp
ATGGCTCTCTCGTCCATCGCGACTCTCACTCGCTTCTCTACTACTACTTCTTCTTCTCACTTCCACTGCATCAGTAAACGGCTCAGACTCATCGCCAGAGCACCAACCAAAAGTCAGCACCGCCAGAAAATGACGTCATCTTCTCGGGAAGCCAAGCAAGAGAAAATCGCAGCTCCTTTCGGCTCGTGGAAGTCCCCAATCACCGCCGATGTCGTCTCCGGCGCCTCCAAGCGGCTCGGCGGCACCGCCGTCGATTCCCACGGCCGCCTCATCTGGCTCGAGTCTCGTCCGTCGGAATCCGGACGAGCGGTTCTGGTGAGAGAACCGGAGAGACCGGAAGACGAACCGGTTGATATCACGCCGAAGGACTACGCGGTTCGAACTCTGGCTCAGGAGTACGGCGGAGGCGCCTTCAGCGTTTCAGGAGATACACTTGTCTTCTCAAATTATAAGGATCAACGCCTTTACAAGCAGTCTTTAAGCTCCAAAGATTCTTCACCAGTACCACTCACTCCAGATTATGGTGGACCAGTTGTTAGTTATGCTGATGGGGTGTTTGATGCACGTTTTAACCGTTTTATCACTGTGAGGGAAGATTGTCGCGAAAGCAGCATGAATTCAATCACAACAATTGTAACAGTAAGGCTTGATGGAAAGGACATTCAGGAATCAGAAACACTCGTAGGTGGATGTGACTTCTATGCCTTCCCGCGTCTGGATCCCAAAGGAGAACGACTTGCATGGATTGAATGGTGCCATCCTAACATGCCATGGGATAGGTCTCAACTTTGGGTTGGGTATATATCTGAGAAAGGGGAAGTCTCAAAACGAGTCTGTGTTTCTGGCTCTGATCCTGCAATTAAGGAATCTCCAATTGAACCCAAGTGGTCCGCAAAAGGGGAACTATTTTTCATTACTGACAGAAAAGGTGGGTTTTGGAATCTCTATAAATGGATTGAGTCTACTAATGAGGTCGTATCAGTTTATTCTTTGGATGCTGAATTTTCCAAACCACTATGGGTTTTTGGCTTGAATTCTTATGAATTTATTGAGAGCCATGAACAAAAGAACCTTATTGCTTGCAGCTATAGGAAAAACGGGAGATCATATCTTGGAATTGTGGATGATATTCAGGGCTCATTATCCCTGCTTAATATTCCTTTCACGGATATTGATAATATTACTTTGGGGTTCAATTGCCTATATGTGGAAGGAGCATCAGAAGTTCATCCATTGTCTGTTGCTAAGGTGACATTAGATGATCAAAAATCAAAAGCAGTTGAATTCAAGATTATTTGGTCTTCTTCACCCGATTGCTTAAAATATGAGTCCTACTTCAGCCTACCTGAATTAATTGAGTTCCCAACAGAAGTTCCTGGTCAAACTGCCTTTGCATACTTTTATCCACCATCAAATCCTAGCTACCAAGCAACTAAGGATGAAAAGCCTCCACTATTACTGAAAAGCCATGGAGGACCCACAGCTGAAGCACGTGGAATTTTAAATCTAAGTGTTCAGTACTGGACTAGTCGTGGTTGGGCATTCGTTGATGTTAACTATGGTGGAAGCACTGGTTATGGCAGAGAATATCGTGAAAGGCTGTTGAAGAAATGGGGAATTGTGGATGTAAATGATTGTTGCAGCTGTGCTAGTTATCTGGTAGATGGGAAACGACTTTGTATTACGGGAGGCTCTGCAGGTGGATACACAACCTTAGCTGCACTTGCATTTCGAGACACCTTCAAGGCTGGAGCTTCCTTGTATGGTGTAGCTGACTTAAACATGTTGAGGGCTGAAACTCACAAATTTGAATCTCATTATATGGATAATCTTGTTGGTAGTGATCAGGATTTCTTTGATAGATCACCAATCAACTTTGTTGATAAATTTTCTTGCCCAATAATCTTATTCCAAGGATTGGAAGACAAGGTTGTACCCCCAGATCAAGCTCGTACAATTTACCGTGCTCTGAAGGAAAAGGGCTTGCCAGTTGCTCTCGTGGAGTATGAAGGAGAACAACATGGTTTCCGCAAGGCTGAGAACATTATATTCACTCTGGAACAGCAAATGGTTTTCTTTGCACGTTTAGTTGGACACTTCAAGGTTGCAGATGAGATTACTCCGATCAAAATTGATAACTTGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

715

Amino Acids

79.99

Weight (kDa)

5.88

Isoelectric Point (pI)

40.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 504 - 698 1.3e-43 Prolyl oligopeptidase family
BD-FAE PF20434 550 - 657 1.7e-08 BD-FAE
DLH PF01738 554 - 682 3.6e-08 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36210
fragaria_vesca FvH4_2g34950
malus_domestica MD08G1107000.v1.1 MD08G1107200.v1.1 MD15G1087700.v1.1 MD15G1087800.v1.1
prunus_persica Prupe.1G441500_v2.0.a1
pyrus_communis pycom08g08900 pycom08g08910 pycom15g08240
rosa_chinensis RchiOBHm_Chr6g0307051
rosa_laevigata RLG00000010738
rosa_multiflora Rmu_sc0005634.1_g000006
rosa_roxburghii Rroxscaffold_7G00161200
rosa_rugosa Rorug06G0355500
rosa_samantha Rh6AG467000 Rh6CG482500 Rh6DG468800
rosa_wichuraiana Rw6G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 450
AarI CACCTGC 1 cut(s) 1688
AatII GACGTC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 1688
AccB1I GGYRCC 4 cut(s) 221, 242, 407, 753
AccB7I CCANNNNNTGG 2 cut(s) 521, 1918
AccBSI CCGCTC 1 cut(s) 308
AccI GTMKAC 1 cut(s) 962
AccII CGCG 4 cut(s) 20, 374, 603, 712
AccIII TCCGGA 1 cut(s) 299
AclWI GGATC 4 cut(s) 462, 713, 726, 845
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 8 cut(s) 616, 1001, 1033, 1043, 1283, 1497, 1805, 1886
AcuI CTGAAG 4 cut(s) 397, 1321, 1506, 1991
AcvI CACGTG 1 cut(s) 1493
AcyI GRCGYC 3 cut(s) 134, 222, 408
AfaI GTAC 5 cut(s) 398, 504, 1520, 1935, 1954
AfiI CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1918
AflIII ACRYGT 1 cut(s) 1782
AgeI ACCGGT 1 cut(s) 346
AhlI ACTAGT 1 cut(s) 1526
AjnI CCWGG 1 cut(s) 1375
AloI GAACNNNNNNTCC 2 cut(s) 294, 326
Alw21I GWGCWC 2 cut(s) 103, 1969
Alw26I GTCTC 6 cut(s) 220, 288, 325, 786, 826, 1728
AlwI GGATC 4 cut(s) 462, 713, 726, 845
Ama87I CYCGRG 2 cut(s) 144, 278
Aor13HI TCCGGA 1 cut(s) 299
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 5 cut(s) 170, 609, 1083, 1641, 1718
ApoI RAATTY 8 cut(s) 616, 1001, 1033, 1043, 1283, 1497, 1805, 1886
AseI ATTAAT 1 cut(s) 1352
AsiGI ACCGGT 1 cut(s) 346
AspLEI GCGC 2 cut(s) 224, 410
AspS9I GGNCC 3 cut(s) 527, 888, 1475
AsuHPI GGTGA 6 cut(s) 193, 328, 489, 1261, 1299, 1857
AsuII TTCGAA 1 cut(s) 379
AvaI CYCGRG 2 cut(s) 144, 278
AvaII GGWCC 3 cut(s) 527, 888, 1475
BaeI ACNNNNGTAYC 2 cut(s) 420, 453
BamHI GGATCC 1 cut(s) 718
BanI GGYRCC 4 cut(s) 221, 242, 407, 753
BanII GRGCYC 1 cut(s) 1139
BauI CACGAG 2 cut(s) 184, 1997
BbrPI CACGTG 1 cut(s) 1493
BbsI GAAGAC 4 cut(s) 345, 430, 1293, 1929
Bbv12I GWGCWC 2 cut(s) 103, 1969
BbvI GCAGC 5 cut(s) 182, 621, 1095, 1653, 1705
BccI CCATC 6 cut(s) 23, 542, 644, 765, 1417, 1658
BceAI ACGGC 4 cut(s) 95, 233, 277, 415
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 1 cut(s) 1377
BciVI GTATCC 1 cut(s) 1697
BclI TGATCA 2 cut(s) 1261, 1843
BcoDI GTCTC 6 cut(s) 220, 288, 325, 786, 826, 1728
BcuI ACTAGT 1 cut(s) 1526
BfaI CTAG 3 cut(s) 1419, 1527, 1650
BfmI CTRYAG 2 cut(s) 1087, 1695
BfoI RGCGCY 2 cut(s) 225, 411
BfuAI ACCTGC 1 cut(s) 1688
BfuI GTATCC 1 cut(s) 1697
BisI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1719
BlsI GCNGC 8 cut(s) 172, 234, 242, 266, 611, 1085, 1643, 1720
BmcAI AGTACT 1 cut(s) 1520
Bme1390I CCNGG 1 cut(s) 1377
Bme18I GGWCC 3 cut(s) 527, 888, 1475
BmeT110I CYCGRG 2 cut(s) 144, 278
BmgT120I GGNCC 3 cut(s) 527, 888, 1475
BmiI GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
BmrFI CCNGG 1 cut(s) 1377
BmsI GCATC 4 cut(s) 78, 550, 985, 1226
BpiI GAAGAC 4 cut(s) 345, 430, 1293, 1929
BpmI CTGGAG 2 cut(s) 498, 1770
Bpu10I CCTNAGC 3 cut(s) 390, 1245, 1714
Bpu14I TTCGAA 1 cut(s) 379
BsaAI YACGTR 1 cut(s) 1493
BsaHI GRCGYC 3 cut(s) 134, 222, 408
BsaI GGTCTC 2 cut(s) 325, 786
BsaJI CCNNGG 4 cut(s) 259, 770, 1468, 1912
BsaWI WCCGGW 4 cut(s) 299, 325, 334, 346
Bsc4I CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1918
Bse118I RCCGGY 1 cut(s) 346
Bse1I ACTGG 5 cut(s) 500, 530, 1526, 1576, 1988
BseAI TCCGGA 1 cut(s) 299
BseBI CCWGG 1 cut(s) 1377
BseDI CCNNGG 4 cut(s) 259, 770, 1468, 1912
BseGI GGATG 7 cut(s) 695, 757, 1000, 1129, 1228, 1444, 1630
BseLI CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1918
BseMII CTCAG 4 cut(s) 97, 404, 798, 2028
BseNI ACTGG 5 cut(s) 500, 530, 1526, 1576, 1988
BseX3I CGGCCG 1 cut(s) 262
BseXI GCAGC 5 cut(s) 182, 621, 1095, 1653, 1705
BsgI GTGCAG 1 cut(s) 1704
Bsh1236I CGCG 4 cut(s) 20, 374, 603, 712
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 1 cut(s) 264
BshNI GGYRCC 4 cut(s) 221, 242, 407, 753
BshTI ACCGGT 1 cut(s) 346
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 2 cut(s) 103, 1969
BsiHKCI CYCGRG 2 cut(s) 144, 278
BsiSI CCGG 5 cut(s) 219, 300, 326, 335, 347
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 6 cut(s) 231, 521, 715, 897, 1207, 1918
BsmAI GTCTC 6 cut(s) 220, 288, 325, 786, 826, 1728
BsmBI CGTCTC 1 cut(s) 220
BsmFI GGGAC 1 cut(s) 178
BsmI GAATGC 1 cut(s) 1541
BsnI GGCC 1 cut(s) 264
Bso31I GGTCTC 2 cut(s) 325, 786
BsoBI CYCGRG 2 cut(s) 144, 278
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 3 cut(s) 103, 1139, 1969
Bsp13I TCCGGA 1 cut(s) 299
Bsp143I GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1843, 1862, 1942, 2124
Bsp19I CCATGG 2 cut(s) 770, 1468
Bsp68I TCGCGA 2 cut(s) 20, 603
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 4 cut(s) 96, 403, 799, 2029
BspEI TCCGGA 1 cut(s) 299
BspFNI CGCG 4 cut(s) 20, 374, 603, 712
BspLI GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
BspMAI CTGCAG 1 cut(s) 1699
BspMI ACCTGC 1 cut(s) 1688
BspPI GGATC 4 cut(s) 462, 713, 726, 845
BspT104I TTCGAA 1 cut(s) 379
BspT107I GGYRCC 4 cut(s) 221, 242, 407, 753
BspTNI GGTCTC 2 cut(s) 325, 786
BsrBI CCGCTC 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 346
BsrI ACTGG 5 cut(s) 500, 530, 1526, 1576, 1988
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 4 cut(s) 259, 770, 1468, 1912
BssMI GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1843, 1862, 1942, 2124
BssNI GRCGYC 3 cut(s) 134, 222, 408
BssSI CACGAG 2 cut(s) 184, 1997
BssT1I CCWWGG 3 cut(s) 770, 1468, 1912
Bst2BI CACGAG 2 cut(s) 184, 1997
Bst2UI CCWGG 1 cut(s) 1377
Bst4CI ACNGT 4 cut(s) 575, 586, 640, 1964
BstACI GRCGYC 3 cut(s) 134, 222, 408
BstBAI YACGTR 1 cut(s) 1493
BstBI TTCGAA 1 cut(s) 379
BstC8I GCNNGC 2 cut(s) 1081, 1985
BstDEI CTNAG 8 cut(s) 83, 390, 807, 1245, 1434, 1508, 1714, 2037
BstDSI CCRYGG 3 cut(s) 259, 770, 1468
BstF5I GGATG 7 cut(s) 695, 757, 1000, 1129, 1228, 1444, 1630
BstFNI CGCG 4 cut(s) 20, 374, 603, 712
BstH2I RGCGCY 2 cut(s) 225, 411
BstHHI GCGC 2 cut(s) 224, 410
BstKTI GATC 9 cut(s) 457, 721, 853, 1106, 1264, 1846, 1865, 1945, 2127
BstMAI GTCTC 6 cut(s) 220, 288, 325, 786, 826, 1728
BstMBI GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1843, 1862, 1942, 2124
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 3 cut(s) 414, 609, 1724
BstNI CCWGG 1 cut(s) 1377
BstNSI RCATGY 2 cut(s) 769, 1786
BstSCI CCNGG 1 cut(s) 1375
BstSFI CTRYAG 2 cut(s) 1087, 1695
BstUI CGCG 4 cut(s) 20, 374, 603, 712
BstV1I GCAGC 5 cut(s) 182, 621, 1095, 1653, 1705
BstV2I GAAGAC 4 cut(s) 345, 430, 1293, 1929
BstX2I RGATCY 1 cut(s) 718
BstYI RGATCY 1 cut(s) 718
BstZI CGGCCG 1 cut(s) 262
BsuI GTATCC 1 cut(s) 1697
BsuRI GGCC 1 cut(s) 264
BtgI CCRYGG 3 cut(s) 259, 770, 1468
BtgZI GCGATG 1 cut(s) 76
BtsCI GGATG 7 cut(s) 695, 757, 1000, 1129, 1228, 1444, 1630
BtsI GCAGTG 1 cut(s) 64
BtsIMutI CAGTG 3 cut(s) 64, 582, 1569
BtuMI TCGCGA 2 cut(s) 20, 603
BveI ACCTGC 1 cut(s) 1688
Cac8I GCNNGC 2 cut(s) 1081, 1985
CfoI GCGC 2 cut(s) 224, 410
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 3 cut(s) 527, 888, 1475
CseI GACGC 1 cut(s) 701
Csp6I GTAC 5 cut(s) 397, 503, 1519, 1934, 1953
CspAI ACCGGT 1 cut(s) 346
CviAII CATG 8 cut(s) 613, 741, 766, 771, 1058, 1469, 1783, 2021
CviQI GTAC 5 cut(s) 397, 503, 1519, 1934, 1953
DdeI CTNAG 8 cut(s) 83, 390, 807, 1245, 1434, 1508, 1714, 2037
DinI GGCGCC 2 cut(s) 223, 409
DpnI GATC 9 cut(s) 456, 720, 852, 1105, 1263, 1845, 1864, 1944, 2126
DpnII GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1843, 1862, 1942, 2124
DraI TTTAAA 1 cut(s) 1503
EaeI YGGCCR 1 cut(s) 262
EagI CGGCCG 1 cut(s) 262
EciI GGCGGA 1 cut(s) 417
EclXI CGGCCG 1 cut(s) 262
Eco130I CCWWGG 3 cut(s) 770, 1468, 1912
Eco24I GRGCYC 1 cut(s) 1139
Eco31I GGTCTC 2 cut(s) 325, 786
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 3 cut(s) 527, 888, 1475
Eco52I CGGCCG 1 cut(s) 262
Eco57I CTGAAG 4 cut(s) 397, 1321, 1506, 1991
Eco72I CACGTG 1 cut(s) 1493
Eco88I CYCGRG 2 cut(s) 144, 278
EcoO109I RGGNCCY 1 cut(s) 1475
EcoRI GAATTC 3 cut(s) 616, 1033, 1283
EcoRII CCWGG 1 cut(s) 1375
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 3 cut(s) 770, 1468, 1912
EcoT38I GRGCYC 1 cut(s) 1139
EgeI GGCGCC 2 cut(s) 223, 409
EheI GGCGCC 2 cut(s) 223, 409
ErhI CCWWGG 3 cut(s) 770, 1468, 1912
Esp3I CGTCTC 1 cut(s) 220
FaeI CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1786, 2024
FaqI GGGAC 1 cut(s) 178
FatI CATG 8 cut(s) 612, 740, 765, 770, 1057, 1468, 1782, 2020
FauI CCCGC 1 cut(s) 717
FbaI TGATCA 2 cut(s) 1261, 1843
FblI GTMKAC 1 cut(s) 962
Fnu4HI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1719
FokI GGATG 7 cut(s) 702, 744, 1007, 1136, 1215, 1451, 1637
FriOI GRGCYC 1 cut(s) 1139
Fsp4HI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1719
FspBI CTAG 3 cut(s) 1419, 1527, 1650
GlaI GCGC 2 cut(s) 223, 409
GluI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1719
GsuI CTGGAG 2 cut(s) 498, 1770
HaeII RGCGCY 2 cut(s) 225, 411
HaeIII GGCC 1 cut(s) 264
HapII CCGG 5 cut(s) 219, 300, 326, 335, 347
HgaI GACGC 1 cut(s) 701
HhaI GCGC 2 cut(s) 224, 410
Hin1I GRCGYC 3 cut(s) 134, 222, 408
Hin1II CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1786, 2024
Hin6I GCGC 2 cut(s) 222, 408
HinP1I GCGC 2 cut(s) 222, 408
HincII GTYRAC 1 cut(s) 1555
HindII GTYRAC 1 cut(s) 1555
HpaI GTTAAC 1 cut(s) 1555
HpaII CCGG 5 cut(s) 219, 300, 326, 335, 347
HphI GGTGA 6 cut(s) 193, 328, 489, 1261, 1299, 1857
Hpy166II GTNNAC 4 cut(s) 77, 527, 963, 1555
Hpy188I TCNGA 8 cut(s) 86, 295, 673, 808, 850, 1222, 1971, 2124
Hpy8I GTNNAC 4 cut(s) 77, 527, 963, 1555
Hpy99I CGWCG 2 cut(s) 254, 295
HpyAV CCTTC 7 cut(s) 358, 421, 715, 1205, 1750, 1966, 2003
HpyCH4III ACNGT 4 cut(s) 575, 586, 640, 1964
HpyCH4IV ACGT 4 cut(s) 134, 565, 1492, 2083
HpyF10VI GCNNNNNNNGC 3 cut(s) 414, 609, 1724
HpyF3I CTNAG 8 cut(s) 83, 390, 807, 1245, 1434, 1508, 1714, 2037
HpySE526I ACGT 4 cut(s) 134, 565, 1492, 2083
Hsp92I GRCGYC 3 cut(s) 134, 222, 408
Hsp92II CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1786, 2024
HspAI GCGC 2 cut(s) 222, 408
KasI GGCGCC 2 cut(s) 221, 407
Kpn2I TCCGGA 1 cut(s) 299
Ksp22I TGATCA 2 cut(s) 1261, 1843
KspAI GTTAAC 1 cut(s) 1555
Kzo9I GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1843, 1862, 1942, 2124
LmnI GCTCC 4 cut(s) 178, 488, 1214, 1751
Lsp1109I GCAGC 5 cut(s) 182, 621, 1095, 1653, 1705
LweI GCATC 4 cut(s) 78, 550, 985, 1226
MaeI CTAG 3 cut(s) 1419, 1527, 1650
MaeII ACGT 4 cut(s) 134, 565, 1492, 2083
MaeIII GTNAC 3 cut(s) 634, 692, 1249
MalI GATC 9 cut(s) 456, 720, 852, 1105, 1263, 1845, 1864, 1944, 2126
MbiI CCGCTC 1 cut(s) 308
MboI GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1843, 1862, 1942, 2124
MfeI CAATTG 3 cut(s) 630, 873, 1195
MflI RGATCY 1 cut(s) 718
MhlI GDGCHC 3 cut(s) 103, 1139, 1969
Mly113I GGCGCC 2 cut(s) 222, 408
MlyI GAGTC 6 cut(s) 16, 81, 290, 840, 968, 1337
MmeI TCCRAC 2 cut(s) 273, 2071
MnlI CCTC 9 cut(s) 235, 278, 398, 582, 964, 1458, 1466, 1682, 1782
MroI TCCGGA 1 cut(s) 299
MspA1I CMGCKG 2 cut(s) 1484, 1644
MspI CCGG 5 cut(s) 219, 300, 326, 335, 347
MspR9I CCNGG 1 cut(s) 1377
MunI CAATTG 3 cut(s) 630, 873, 1195
Mva1269I GAATGC 1 cut(s) 1541
MvaI CCWGG 1 cut(s) 1377
MvnI CGCG 4 cut(s) 20, 374, 603, 712
MwoI GCNNNNNNNGC 3 cut(s) 414, 609, 1724
NarI GGCGCC 2 cut(s) 222, 408
NcoI CCATGG 2 cut(s) 770, 1468
NdeII GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1843, 1862, 1942, 2124
NlaIII CATG 8 cut(s) 616, 744, 769, 774, 1061, 1472, 1786, 2024
NlaIV GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 2 cut(s) 692, 1249
NruI TCGCGA 2 cut(s) 20, 603
NspI RCATGY 2 cut(s) 769, 1786
NspV TTCGAA 1 cut(s) 379
PaeR7I CTCGAG 1 cut(s) 278
PaqCI CACCTGC 1 cut(s) 1688
PciI ACATGT 1 cut(s) 1782
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 571
PctI GAATGC 1 cut(s) 1541
PfeI GAWTC 7 cut(s) 254, 296, 491, 668, 866, 940, 1811
PflMI CCANNNNNTGG 2 cut(s) 521, 1918
PinAI ACCGGT 1 cut(s) 346
PkrI GCNGC 8 cut(s) 172, 234, 242, 266, 611, 1085, 1643, 1720
PleI GAGTC 6 cut(s) 16, 81, 289, 839, 967, 1336
PluTI GGCGCC 2 cut(s) 225, 411
PmaCI CACGTG 1 cut(s) 1493
PmlI CACGTG 1 cut(s) 1493
PpsI GAGTC 6 cut(s) 16, 81, 289, 839, 967, 1336
Ppu21I YACGTR 1 cut(s) 1493
PpuMI RGGWCCY 1 cut(s) 1475
PscI ACATGT 1 cut(s) 1782
PshBI ATTAAT 1 cut(s) 1352
PsiI TTATAA 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 1475
Psp6I CCWGG 1 cut(s) 1375
PspCI CACGTG 1 cut(s) 1493
PspGI CCWGG 1 cut(s) 1375
PspN4I GGNNCC 6 cut(s) 223, 244, 409, 720, 755, 1477
PspPI GGNCC 3 cut(s) 527, 888, 1475
PspPPI RGGWCCY 1 cut(s) 1475
PspXI VCTCGAGB 1 cut(s) 278
PstI CTGCAG 1 cut(s) 1699
PsuI RGATCY 1 cut(s) 718
PvuII CAGCTG 2 cut(s) 1484, 1644
RruI TCGCGA 2 cut(s) 20, 603
RsaI GTAC 5 cut(s) 398, 504, 1520, 1935, 1954
RsaNI GTAC 5 cut(s) 397, 503, 1519, 1934, 1953
SatI GCNGC 8 cut(s) 171, 233, 241, 265, 610, 1084, 1642, 1719
Sau3AI GATC 9 cut(s) 454, 718, 850, 1103, 1261, 1843, 1862, 1942, 2124
Sau96I GGNCC 3 cut(s) 527, 888, 1475
ScaI AGTACT 1 cut(s) 1520
SchI GAGTC 6 cut(s) 16, 81, 290, 840, 968, 1337
ScrFI CCNGG 1 cut(s) 1377
SduI GDGCHC 3 cut(s) 103, 1139, 1969
SfaNI GCATC 4 cut(s) 78, 550, 985, 1226
SfcI CTRYAG 2 cut(s) 1087, 1695
SfoI GGCGCC 2 cut(s) 223, 409
Sfr274I CTCGAG 1 cut(s) 278
SfuI TTCGAA 1 cut(s) 379
SinI GGWCC 3 cut(s) 527, 888, 1475
SlaI CTCGAG 1 cut(s) 278
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SpeI ACTAGT 1 cut(s) 1526
SspDI GGCGCC 2 cut(s) 221, 407
SspI AATATT 2 cut(s) 1156, 1180
SspMI CTAG 3 cut(s) 1419, 1527, 1650
StyD4I CCNGG 1 cut(s) 1375
StyI CCWWGG 3 cut(s) 770, 1468, 1912
TaaI ACNGT 4 cut(s) 575, 586, 640, 1964
TaiI ACGT 4 cut(s) 137, 568, 1495, 2086
TaqI TCGA 4 cut(s) 252, 279, 379, 1732
TatI WGTACW 1 cut(s) 1518
TauI GCSGC 3 cut(s) 235, 243, 267
TfiI GAWTC 7 cut(s) 254, 296, 491, 668, 866, 940, 1811
TscAI CASTG 3 cut(s) 71, 589, 1576
TseFI GTSAC 2 cut(s) 692, 1249
TseI GCWGC 5 cut(s) 170, 609, 1083, 1641, 1718
Tsp45I GTSAC 2 cut(s) 692, 1249
TspDTI ATGAA 7 cut(s) 629, 901, 1056, 1074, 1217, 1455, 2022
TspGWI ACGGA 2 cut(s) 279, 1181
TspRI CASTG 3 cut(s) 71, 589, 1576
Van91I CCANNNNNTGG 2 cut(s) 521, 1918
VpaK11BI GGWCC 3 cut(s) 527, 888, 1475
VspI ATTAAT 1 cut(s) 1352
XapI RAATTY 8 cut(s) 616, 1001, 1033, 1043, 1283, 1497, 1805, 1886
XceI RCATGY 2 cut(s) 769, 1786
XhoI CTCGAG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 962
XspI CTAG 3 cut(s) 1419, 1527, 1650
ZraI GACGTC 1 cut(s) 135
ZrmI AGTACT 1 cut(s) 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.