RLG00000010738

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0012367
Physical Location & Seq
Reverse (-)
4032331 .. 4037236
4906 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010738

Sequence Viewer

Length: 2172 bp
ATGGCTCTCTCGTCCATCGCTACCCTCACTCGCTTCTCTACTGCTACCATCACTTCTTCTTTTTCTCACGTCCACTCCATCAGTAAACGGCTCAGACTCATCGCCAGAGCACCAACCAAAAGTCAGCACCGCCAGAAAATGACGTCATCTTCTCCGGAAGCCAAGCAAGAGAAAATCGCAGCTCCTTTCGGCTCGTGGAAGTCCCCAATCACCGCCGATGTCGTCTCCGGCGCCTCCAAGCAGCTCGGCGGCACCGCCGTCGATTCCCACGGCCGCCTCATCTGGCTCGAGTCTCGTCCGTCGGAATCCGGACGAGCGGTTCTGGTGAGAGAACCGGAGAGACCGGAAGACGAACCGGTTGATATCACGCCGAAGGACTACGCGGTTCGAACTCTGGCTCAGGAGTACGGCGGAGGCGCCTTCAGCGTTTCGGGAGATACACTTGTCTTCTCAAATTATAACGATCAACGCCTTTACAAGCAGTCTTTAAGCTCCAAAGATTCGTCACCAGTACCACTCACTCCAGATTATGGTGGACCAGTTGTTAGTTATGCTGATGGGGTGTTTGATGCACGTTTTAACCGTTTTATCACTGTGAGAGAAGATTGTCGCGAAAGCAGCATGAATTCAATCACAACAATTGTAACAGTAAAGCTTGATGGAAAGGACATTCAGGAATCAGAAACACTCGTAGGTGGATGTGACTTCTATGCCTTCCCGCGTCTGGATCCCAAAGGAGAACGACTTGCATGGATTGAATGGTGCCATCCTAACATGCCATGGGATAGGTCTCAACTTTGGGTTGGGTATATATCTGAGAAAGGGGAAGTCTCAAAACGAGTCTGTGTTTCTGGCTCTGATCCTGCAATTAAGGAATCTCCAATTGAACCCAAGTGGTCCGCAAAAGGGGAACTATTTTTCATTACTGACAGAAAAAGTGGGTTTTGGAATCTCTATAAATGGGTTGAGTCTACTAATGAGGTCATATCAGTTTATTCTTTGGATGCTGAATTTTCCAAACCATTATGGGTTTTTGGCTTGAATTCTTATGAATTTATTCAGAGCCATGAACATAAGAACCTTATTGCTTGCAGCTATAGGAAAAACGGGAGATCATATCTTGGAATTGTGGATGATGTTCAGGGCTCATTATCCCTGCTTAATAGTCCTTTCACGGATATTGATAATATTACTTTGGGGATTAATTGCCTATATGTGGAAGGAGCATCAGAAGTTCATCCATTGTCTGTTGCTAAGGTGACATTAGATGATCAAAAATCAAAAGCAGTTGAATTCAAGATTATTTGGTCTTCTTCACCCGATTGCTTAAAATATGAGTCCTACTTCAGCCTACCTGAATTAATTGAGTTCCCAACAGAAGTTCCTGGTCAAACTGCCTTTGCATACTATTATCCACCATCAAATCCTAGCTACCAAGCAACTAAGGACGAAAAGCCTCCACTATTACTGAAAAGCCATGGAGGACCCACAGATGAAGCACGTGGAATTTTAAATCTAAGTGTTCAGTACTGGACTAGTCGTGGTTGGGCATTCGTTGATGTTAACTATGGTGGAAGCACTGGTTATGGCAGAGAATATCGTGAAAGGCTGTTGAAGAAATGGGGAATTGTGGATGTAAATGATTGTTGTAGCTGTGCTAGTTATCTGGTGGAATCTGGAAAGGTAGATGGGAAACGACTTTGTATTACTGGAGGCTCTGCAGGTGGATACACAACCTTAGCTGCACTTGCATTTCGAGACACCTTCAAGGCTGGAGCTTCCTTGTATGGTGTAGCTGACTTAAACATGTTGAGGGCCGAAACTCACAAATTTGAATCTCATTATATGGATAATCTTGTTGGTAGTGATCAGGATTTCTTTGATAGATCACCAATCAACTTTGTTGATAAATTTTCTTGCCCAATAATCTTATTCCAAGGATTGGAAGACAAGGTTGTACCCCCAGATCAAGCTCGTACAATTTACCGTGCTCTGAAGGAAAAGGGCTTGCCAGTTGCTCTTGTGGAGTATGAAGGAGAACAACATGGTTTCCGCAAGGCTGAGAACATTATATTCACTCTGGAACAGCAAATGGTTTTCTTTGCACGTTTAGTTGGACACTTCAAGGTTGCAGATGAGATTACTCCGATCAAAATTGATAACTTGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

724

Amino Acids

80.65

Weight (kDa)

5.7

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 507 - 706 2.7e-47 Prolyl oligopeptidase family
BD-FAE PF20434 545 - 665 8.9e-09 BD-FAE
DLH PF01738 554 - 690 5.3e-09 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36210
fragaria_vesca FvH4_2g34950
malus_domestica MD08G1107000.v1.1 MD08G1107200.v1.1 MD15G1087700.v1.1 MD15G1087800.v1.1
prunus_persica Prupe.1G441500_v2.0.a1
pyrus_communis pycom08g08900 pycom08g08910 pycom15g08240
rosa_chinensis RchiOBHm_Chr6g0307051
rosa_laevigata RLG00000010738
rosa_multiflora Rmu_sc0005634.1_g000006
rosa_roxburghii Rroxscaffold_7G00161200
rosa_rugosa Rorug06G0355500
rosa_samantha Rh6AG467000 Rh6CG482500 Rh6DG468800
rosa_wichuraiana Rw6G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 459
AarI CACCTGC 1 cut(s) 1712
AatII GACGTC 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 1712
AccB1I GGYRCC 4 cut(s) 230, 251, 416, 762
AccB7I CCANNNNNTGG 2 cut(s) 530, 1942
AccBSI CCGCTC 1 cut(s) 317
AccI GTMKAC 1 cut(s) 971
AccII CGCG 3 cut(s) 383, 612, 721
AccIII TCCGGA 2 cut(s) 154, 308
AclWI GGATC 3 cut(s) 722, 735, 854
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 8 cut(s) 625, 1010, 1042, 1052, 1292, 1506, 1829, 1910
AcuI CTGAAG 3 cut(s) 406, 1330, 2015
AcvI CACGTG 1 cut(s) 1502
AcyI GRCGYC 3 cut(s) 143, 231, 417
AfaI GTAC 5 cut(s) 407, 513, 1529, 1959, 1978
AfiI CCNNNNNNNGG 5 cut(s) 530, 724, 906, 1216, 1942
AflIII ACRYGT 1 cut(s) 1806
AgeI ACCGGT 1 cut(s) 355
AhlI ACTAGT 1 cut(s) 1535
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 1384
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
Alw21I GWGCWC 2 cut(s) 112, 1993
Alw26I GTCTC 6 cut(s) 229, 297, 334, 795, 835, 1752
AlwI GGATC 3 cut(s) 722, 735, 854
Ama87I CYCGRG 1 cut(s) 287
Aor13HI TCCGGA 2 cut(s) 154, 308
AoxI GGCC 2 cut(s) 271, 1815
ApeKI GCWGC 5 cut(s) 179, 241, 618, 1092, 1742
ApoI RAATTY 8 cut(s) 625, 1010, 1042, 1052, 1292, 1506, 1829, 1910
AseI ATTAAT 2 cut(s) 1203, 1361
AsiGI ACCGGT 1 cut(s) 355
Asp700I GAANNNNTTC 1 cut(s) 1056
AspLEI GCGC 2 cut(s) 233, 419
AspS9I GGNCC 4 cut(s) 536, 897, 1484, 1815
AsuHPI GGTGA 6 cut(s) 202, 337, 498, 1270, 1308, 1881
AsuII TTCGAA 1 cut(s) 388
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 3 cut(s) 536, 897, 1484
BaeI ACNNNNGTAYC 2 cut(s) 429, 462
BamHI GGATCC 1 cut(s) 727
BanI GGYRCC 4 cut(s) 230, 251, 416, 762
BanII GRGCYC 1 cut(s) 1148
BauI CACGAG 1 cut(s) 193
BbrPI CACGTG 1 cut(s) 1502
BbsI GAAGAC 4 cut(s) 354, 439, 1302, 1953
Bbv12I GWGCWC 2 cut(s) 112, 1993
BbvI GCAGC 5 cut(s) 191, 253, 630, 1104, 1729
BccI CCATC 8 cut(s) 23, 56, 86, 551, 653, 774, 1426, 1682
BceAI ACGGC 4 cut(s) 104, 242, 286, 424
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 1 cut(s) 1386
BciVI GTATCC 1 cut(s) 1721
BclI TGATCA 2 cut(s) 1270, 1867
BcoDI GTCTC 6 cut(s) 229, 297, 334, 795, 835, 1752
BcuI ACTAGT 1 cut(s) 1535
BfaI CTAG 3 cut(s) 1428, 1536, 1659
BfmI CTRYAG 2 cut(s) 1096, 1719
BfoI RGCGCY 2 cut(s) 234, 420
BfuAI ACCTGC 1 cut(s) 1712
BfuI GTATCC 1 cut(s) 1721
BisI GCNGC 7 cut(s) 180, 242, 250, 274, 619, 1093, 1743
BlsI GCNGC 7 cut(s) 181, 243, 251, 275, 620, 1094, 1744
BmcAI AGTACT 1 cut(s) 1529
Bme1390I CCNGG 1 cut(s) 1386
Bme18I GGWCC 3 cut(s) 536, 897, 1484
BmeT110I CYCGRG 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 4 cut(s) 536, 897, 1484, 1815
BmiI GGNNCC 6 cut(s) 232, 253, 418, 729, 764, 1486
BmrFI CCNGG 1 cut(s) 1386
BmsI GCATC 3 cut(s) 559, 994, 1235
BpiI GAAGAC 4 cut(s) 354, 439, 1302, 1953
BpmI CTGGAG 3 cut(s) 507, 1731, 1794
Bpu10I CCTNAGC 3 cut(s) 399, 1254, 1738
Bpu14I TTCGAA 1 cut(s) 388
BsaAI YACGTR 1 cut(s) 1502
BsaHI GRCGYC 3 cut(s) 143, 231, 417
BsaI GGTCTC 2 cut(s) 334, 795
BsaJI CCNNGG 4 cut(s) 268, 779, 1477, 1936
BsaWI WCCGGW 5 cut(s) 154, 308, 334, 343, 355
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 5 cut(s) 530, 724, 906, 1216, 1942
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 6 cut(s) 509, 539, 1535, 1585, 1714, 2012
BseAI TCCGGA 2 cut(s) 154, 308
BseBI CCWGG 1 cut(s) 1386
BseDI CCNNGG 4 cut(s) 268, 779, 1477, 1936
BseGI GGATG 6 cut(s) 704, 766, 1009, 1138, 1237, 1639
BseLI CCNNNNNNNGG 5 cut(s) 530, 724, 906, 1216, 1942
BseMII CTCAG 4 cut(s) 106, 413, 807, 2052
BseNI ACTGG 6 cut(s) 509, 539, 1535, 1585, 1714, 2012
BseX3I CGGCCG 1 cut(s) 271
BseXI GCAGC 5 cut(s) 191, 253, 630, 1104, 1729
BsgI GTGCAG 1 cut(s) 1728
Bsh1236I CGCG 3 cut(s) 383, 612, 721
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 2 cut(s) 273, 1817
BshNI GGYRCC 4 cut(s) 230, 251, 416, 762
BshTI ACCGGT 1 cut(s) 355
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 2 cut(s) 112, 1993
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 6 cut(s) 155, 228, 309, 335, 344, 356
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 5 cut(s) 530, 724, 906, 1216, 1942
BsmAI GTCTC 6 cut(s) 229, 297, 334, 795, 835, 1752
BsmBI CGTCTC 1 cut(s) 229
BsmFI GGGAC 1 cut(s) 187
BsmI GAATGC 1 cut(s) 1550
BsnI GGCC 2 cut(s) 273, 1817
Bso31I GGTCTC 2 cut(s) 334, 795
BsoBI CYCGRG 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 388
Bsp1286I GDGCHC 3 cut(s) 112, 1148, 1993
Bsp13I TCCGGA 2 cut(s) 154, 308
Bsp143I GATC 9 cut(s) 463, 727, 859, 1112, 1270, 1867, 1886, 1966, 2148
Bsp19I CCATGG 2 cut(s) 779, 1477
Bsp68I TCGCGA 1 cut(s) 612
BspANI GGCC 2 cut(s) 273, 1817
BspCNI CTCAG 4 cut(s) 105, 412, 808, 2053
BspEI TCCGGA 2 cut(s) 154, 308
BspFNI CGCG 3 cut(s) 383, 612, 721
BspLI GGNNCC 6 cut(s) 232, 253, 418, 729, 764, 1486
BspMAI CTGCAG 1 cut(s) 1723
BspMI ACCTGC 1 cut(s) 1712
BspPI GGATC 3 cut(s) 722, 735, 854
BspT104I TTCGAA 1 cut(s) 388
BspT107I GGYRCC 4 cut(s) 230, 251, 416, 762
BspTNI GGTCTC 2 cut(s) 334, 795
BsrBI CCGCTC 1 cut(s) 317
BsrFI RCCGGY 1 cut(s) 355
BsrI ACTGG 6 cut(s) 509, 539, 1535, 1585, 1714, 2012
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 4 cut(s) 268, 779, 1477, 1936
BssMI GATC 9 cut(s) 463, 727, 859, 1112, 1270, 1867, 1886, 1966, 2148
BssNI GRCGYC 3 cut(s) 143, 231, 417
BssSI CACGAG 1 cut(s) 193
BssT1I CCWWGG 3 cut(s) 779, 1477, 1936
Bst2BI CACGAG 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 1386
Bst4CI ACNGT 4 cut(s) 584, 595, 649, 1988
BstACI GRCGYC 3 cut(s) 143, 231, 417
BstBAI YACGTR 1 cut(s) 1502
BstBI TTCGAA 1 cut(s) 388
BstC8I GCNNGC 2 cut(s) 1090, 2009
BstDEI CTNAG 8 cut(s) 92, 399, 816, 1254, 1443, 1517, 1738, 2061
BstDSI CCRYGG 3 cut(s) 268, 779, 1477
BstF5I GGATG 6 cut(s) 704, 766, 1009, 1138, 1237, 1639
BstFNI CGCG 3 cut(s) 383, 612, 721
BstH2I RGCGCY 2 cut(s) 234, 420
BstHHI GCGC 2 cut(s) 233, 419
BstKTI GATC 9 cut(s) 466, 730, 862, 1115, 1273, 1870, 1889, 1969, 2151
BstMAI GTCTC 6 cut(s) 229, 297, 334, 795, 835, 1752
BstMBI GATC 9 cut(s) 463, 727, 859, 1112, 1270, 1867, 1886, 1966, 2148
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 3 cut(s) 423, 618, 1748
BstNI CCWGG 1 cut(s) 1386
BstNSI RCATGY 2 cut(s) 778, 1810
BstSCI CCNGG 1 cut(s) 1384
BstSFI CTRYAG 2 cut(s) 1096, 1719
BstUI CGCG 3 cut(s) 383, 612, 721
BstV1I GCAGC 5 cut(s) 191, 253, 630, 1104, 1729
BstV2I GAAGAC 4 cut(s) 354, 439, 1302, 1953
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
BstZI CGGCCG 1 cut(s) 271
BsuI GTATCC 1 cut(s) 1721
BsuRI GGCC 2 cut(s) 273, 1817
BtgI CCRYGG 3 cut(s) 268, 779, 1477
BtgZI GCGATG 1 cut(s) 85
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 6 cut(s) 704, 766, 1009, 1138, 1237, 1639
BtsIMutI CAGTG 2 cut(s) 591, 1578
BtuMI TCGCGA 1 cut(s) 612
BveI ACCTGC 1 cut(s) 1712
Cac8I GCNNGC 2 cut(s) 1090, 2009
CfoI GCGC 2 cut(s) 233, 419
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 4 cut(s) 536, 897, 1484, 1815
CseI GACGC 1 cut(s) 710
Csp6I GTAC 5 cut(s) 406, 512, 1528, 1958, 1977
CspAI ACCGGT 1 cut(s) 355
CviAII CATG 8 cut(s) 622, 750, 775, 780, 1067, 1478, 1807, 2045
CviQI GTAC 5 cut(s) 406, 512, 1528, 1958, 1977
DdeI CTNAG 8 cut(s) 92, 399, 816, 1254, 1443, 1517, 1738, 2061
DinI GGCGCC 2 cut(s) 232, 418
DpnI GATC 9 cut(s) 465, 729, 861, 1114, 1272, 1869, 1888, 1968, 2150
DpnII GATC 9 cut(s) 463, 727, 859, 1112, 1270, 1867, 1886, 1966, 2148
DraI TTTAAA 1 cut(s) 1512
EaeI YGGCCR 1 cut(s) 271
EagI CGGCCG 1 cut(s) 271
EciI GGCGGA 1 cut(s) 426
EclXI CGGCCG 1 cut(s) 271
Eco130I CCWWGG 3 cut(s) 779, 1477, 1936
Eco24I GRGCYC 1 cut(s) 1148
Eco31I GGTCTC 2 cut(s) 334, 795
Eco32I GATATC 1 cut(s) 364
Eco47I GGWCC 3 cut(s) 536, 897, 1484
Eco52I CGGCCG 1 cut(s) 271
Eco57I CTGAAG 3 cut(s) 406, 1330, 2015
Eco72I CACGTG 1 cut(s) 1502
Eco88I CYCGRG 1 cut(s) 287
EcoO109I RGGNCCY 1 cut(s) 1484
EcoRI GAATTC 3 cut(s) 625, 1042, 1292
EcoRII CCWGG 1 cut(s) 1384
EcoRV GATATC 1 cut(s) 364
EcoT14I CCWWGG 3 cut(s) 779, 1477, 1936
EcoT38I GRGCYC 1 cut(s) 1148
EgeI GGCGCC 2 cut(s) 232, 418
EheI GGCGCC 2 cut(s) 232, 418
ErhI CCWWGG 3 cut(s) 779, 1477, 1936
Esp3I CGTCTC 1 cut(s) 229
FaeI CATG 8 cut(s) 625, 753, 778, 783, 1070, 1481, 1810, 2048
FaqI GGGAC 1 cut(s) 187
FatI CATG 8 cut(s) 621, 749, 774, 779, 1066, 1477, 1806, 2044
FauI CCCGC 1 cut(s) 726
FbaI TGATCA 2 cut(s) 1270, 1867
FblI GTMKAC 1 cut(s) 971
Fnu4HI GCNGC 7 cut(s) 180, 242, 250, 274, 619, 1093, 1743
FokI GGATG 6 cut(s) 711, 753, 1016, 1145, 1224, 1646
FriOI GRGCYC 1 cut(s) 1148
Fsp4HI GCNGC 7 cut(s) 180, 242, 250, 274, 619, 1093, 1743
FspBI CTAG 3 cut(s) 1428, 1536, 1659
GlaI GCGC 2 cut(s) 232, 418
GluI GCNGC 7 cut(s) 180, 242, 250, 274, 619, 1093, 1743
GsuI CTGGAG 3 cut(s) 507, 1731, 1794
HaeII RGCGCY 2 cut(s) 234, 420
HaeIII GGCC 2 cut(s) 273, 1817
HapII CCGG 6 cut(s) 155, 228, 309, 335, 344, 356
HgaI GACGC 1 cut(s) 710
HhaI GCGC 2 cut(s) 233, 419
Hin1I GRCGYC 3 cut(s) 143, 231, 417
Hin1II CATG 8 cut(s) 625, 753, 778, 783, 1070, 1481, 1810, 2048
Hin6I GCGC 2 cut(s) 231, 417
HinP1I GCGC 2 cut(s) 231, 417
HincII GTYRAC 1 cut(s) 1564
HindII GTYRAC 1 cut(s) 1564
HindIII AAGCTT 1 cut(s) 653
HpaI GTTAAC 1 cut(s) 1564
HpaII CCGG 6 cut(s) 155, 228, 309, 335, 344, 356
HphI GGTGA 6 cut(s) 202, 337, 498, 1270, 1308, 1881
Hpy166II GTNNAC 5 cut(s) 73, 86, 536, 972, 1564
Hpy188I TCNGA 9 cut(s) 95, 304, 682, 817, 859, 1062, 1231, 1995, 2148
Hpy8I GTNNAC 5 cut(s) 73, 86, 536, 972, 1564
Hpy99I CGWCG 2 cut(s) 263, 304
HpyAV CCTTC 7 cut(s) 367, 430, 724, 1214, 1774, 1990, 2027
HpyCH4III ACNGT 4 cut(s) 584, 595, 649, 1988
HpyCH4IV ACGT 5 cut(s) 69, 143, 574, 1501, 2107
HpyF10VI GCNNNNNNNGC 3 cut(s) 423, 618, 1748
HpyF3I CTNAG 8 cut(s) 92, 399, 816, 1254, 1443, 1517, 1738, 2061
HpySE526I ACGT 5 cut(s) 69, 143, 574, 1501, 2107
Hsp92I GRCGYC 3 cut(s) 143, 231, 417
Hsp92II CATG 8 cut(s) 625, 753, 778, 783, 1070, 1481, 1810, 2048
HspAI GCGC 2 cut(s) 231, 417
KasI GGCGCC 2 cut(s) 230, 416
Kpn2I TCCGGA 2 cut(s) 154, 308
Ksp22I TGATCA 2 cut(s) 1270, 1867
KspAI GTTAAC 1 cut(s) 1564
Kzo9I GATC 9 cut(s) 463, 727, 859, 1112, 1270, 1867, 1886, 1966, 2148
LmnI GCTCC 4 cut(s) 187, 497, 1223, 1775
Lsp1109I GCAGC 5 cut(s) 191, 253, 630, 1104, 1729
LweI GCATC 3 cut(s) 559, 994, 1235
MaeI CTAG 3 cut(s) 1428, 1536, 1659
MaeII ACGT 5 cut(s) 69, 143, 574, 1501, 2107
MaeIII GTNAC 4 cut(s) 504, 643, 701, 1258
MalI GATC 9 cut(s) 465, 729, 861, 1114, 1272, 1869, 1888, 1968, 2150
MbiI CCGCTC 1 cut(s) 317
MboI GATC 9 cut(s) 463, 727, 859, 1112, 1270, 1867, 1886, 1966, 2148
MboII GAAGA 9 cut(s) 48, 141, 359, 439, 614, 1302, 1305, 1627, 1958
MfeI CAATTG 2 cut(s) 639, 882
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 3 cut(s) 112, 1148, 1993
Mly113I GGCGCC 2 cut(s) 231, 417
MlyI GAGTC 5 cut(s) 90, 299, 849, 977, 1346
MmeI TCCRAC 2 cut(s) 282, 2095
MnlI CCTC 9 cut(s) 35, 244, 287, 407, 973, 1467, 1475, 1706, 1806
MroI TCCGGA 2 cut(s) 154, 308
MroXI GAANNNNTTC 1 cut(s) 1056
MspI CCGG 6 cut(s) 155, 228, 309, 335, 344, 356
MspR9I CCNGG 1 cut(s) 1386
MunI CAATTG 2 cut(s) 639, 882
Mva1269I GAATGC 1 cut(s) 1550
MvaI CCWGG 1 cut(s) 1386
MvnI CGCG 3 cut(s) 383, 612, 721
MwoI GCNNNNNNNGC 3 cut(s) 423, 618, 1748
NarI GGCGCC 2 cut(s) 231, 417
NcoI CCATGG 2 cut(s) 779, 1477
NdeII GATC 9 cut(s) 463, 727, 859, 1112, 1270, 1867, 1886, 1966, 2148
NlaIII CATG 8 cut(s) 625, 753, 778, 783, 1070, 1481, 1810, 2048
NlaIV GGNNCC 6 cut(s) 232, 253, 418, 729, 764, 1486
NmeAIII GCCGAG 1 cut(s) 225
NmuCI GTSAC 3 cut(s) 504, 701, 1258
NruI TCGCGA 1 cut(s) 612
NspI RCATGY 2 cut(s) 778, 1810
NspV TTCGAA 1 cut(s) 388
PaeR7I CTCGAG 1 cut(s) 287
PaqCI CACCTGC 1 cut(s) 1712
PciI ACATGT 1 cut(s) 1806
PcsI WCGNNNNNNNCGW 1 cut(s) 580
PctI GAATGC 1 cut(s) 1550
PdmI GAANNNNTTC 1 cut(s) 1056
PfeI GAWTC 8 cut(s) 263, 305, 500, 677, 875, 949, 1673, 1835
PflMI CCANNNNNTGG 2 cut(s) 530, 1942
PinAI ACCGGT 1 cut(s) 355
PkrI GCNGC 7 cut(s) 181, 243, 251, 275, 620, 1094, 1744
PleI GAGTC 5 cut(s) 90, 298, 848, 976, 1345
PluTI GGCGCC 2 cut(s) 234, 420
PmaCI CACGTG 1 cut(s) 1502
PmlI CACGTG 1 cut(s) 1502
PpsI GAGTC 5 cut(s) 90, 298, 848, 976, 1345
Ppu21I YACGTR 1 cut(s) 1502
PpuMI RGGWCCY 1 cut(s) 1484
PscI ACATGT 1 cut(s) 1806
PshBI ATTAAT 2 cut(s) 1203, 1361
PsiI TTATAA 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 1484
Psp6I CCWGG 1 cut(s) 1384
PspCI CACGTG 1 cut(s) 1502
PspGI CCWGG 1 cut(s) 1384
PspN4I GGNNCC 6 cut(s) 232, 253, 418, 729, 764, 1486
PspPI GGNCC 4 cut(s) 536, 897, 1484, 1815
PspPPI RGGWCCY 1 cut(s) 1484
PspXI VCTCGAGB 1 cut(s) 287
PstI CTGCAG 1 cut(s) 1723
PsuI RGATCY 1 cut(s) 727
RruI TCGCGA 1 cut(s) 612
RsaI GTAC 5 cut(s) 407, 513, 1529, 1959, 1978
RsaNI GTAC 5 cut(s) 406, 512, 1528, 1958, 1977
SatI GCNGC 7 cut(s) 180, 242, 250, 274, 619, 1093, 1743
Sau3AI GATC 9 cut(s) 463, 727, 859, 1112, 1270, 1867, 1886, 1966, 2148
Sau96I GGNCC 4 cut(s) 536, 897, 1484, 1815
ScaI AGTACT 1 cut(s) 1529
SchI GAGTC 5 cut(s) 90, 299, 849, 977, 1346
ScrFI CCNGG 1 cut(s) 1386
SduI GDGCHC 3 cut(s) 112, 1148, 1993
SfaNI GCATC 3 cut(s) 559, 994, 1235
SfcI CTRYAG 2 cut(s) 1096, 1719
SfoI GGCGCC 2 cut(s) 232, 418
Sfr274I CTCGAG 1 cut(s) 287
SfuI TTCGAA 1 cut(s) 388
SinI GGWCC 3 cut(s) 536, 897, 1484
SlaI CTCGAG 1 cut(s) 287
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SpeI ACTAGT 1 cut(s) 1535
SspDI GGCGCC 2 cut(s) 230, 416
SspI AATATT 1 cut(s) 1189
SspMI CTAG 3 cut(s) 1428, 1536, 1659
StyD4I CCNGG 1 cut(s) 1384
StyI CCWWGG 3 cut(s) 779, 1477, 1936
TaaI ACNGT 4 cut(s) 584, 595, 649, 1988
TaiI ACGT 5 cut(s) 72, 146, 577, 1504, 2110
TaqI TCGA 4 cut(s) 261, 288, 388, 1756
TatI WGTACW 1 cut(s) 1527
TauI GCSGC 2 cut(s) 252, 276
TfiI GAWTC 8 cut(s) 263, 305, 500, 677, 875, 949, 1673, 1835
TscAI CASTG 2 cut(s) 598, 1585
TseFI GTSAC 3 cut(s) 504, 701, 1258
TseI GCWGC 5 cut(s) 179, 241, 618, 1092, 1742
Tsp45I GTSAC 3 cut(s) 504, 701, 1258
TspDTI ATGAA 7 cut(s) 638, 910, 1065, 1083, 1226, 1509, 2046
TspGWI ACGGA 2 cut(s) 288, 1190
TspRI CASTG 2 cut(s) 598, 1585
Van91I CCANNNNNTGG 2 cut(s) 530, 1942
VpaK11BI GGWCC 3 cut(s) 536, 897, 1484
VspI ATTAAT 2 cut(s) 1203, 1361
XapI RAATTY 8 cut(s) 625, 1010, 1042, 1052, 1292, 1506, 1829, 1910
XceI RCATGY 2 cut(s) 778, 1810
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 971
XmnI GAANNNNTTC 1 cut(s) 1056
XspI CTAG 3 cut(s) 1428, 1536, 1659
ZraI GACGTC 1 cut(s) 144
ZrmI AGTACT 1 cut(s) 1529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.