Rroxscaffold_7G00161200

Prolyl oligopeptidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012367
Physical Location & Seq
Reverse (-)
4004411 .. 4011338
6928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00161200.1

Sequence Viewer

Length: 2277 bp
ATGACGTCATCTTCTCTGGAAGCCAAGCAAGAGAAAATCACAGCTCCTTTCGGCTCTTGGAAATCCCCAATCACCGCCGATGTCGTCTCCGGCGCCTCCAAGCGGCTCGGAGGCACCGCCGTCGATTCCCACGGCCGCCTCATCTGGCTCGAGTCTCGTCCGTCGGAATCCGGACGAGCGGTTCTGGTGAGAGAACCGGAGAGACCGGAAGACGAACCGGTTGATATCACGCCGAAGGACTACGCGGTTCGAACTCTGGCTCAGGAGTACGGCGGAGGCGCCTTCAGCGTTTCAGGAGATACACTTGTCTTCTCAAATTATAAGGATCAACGCCTTTACAAGCAGTCTTTAAGCTCCAAAGATTCTTCACCAGTACCACTCACTCCAGATTATGGTGGACCAGTTGTTAGTTATGCTGATGGGGTGTTTGATGCACGTTTTAACCGTTTTATCACTGTGAGGGAAGATTGTCGCGAAAGCAGCATGAATTCAATCACAACAATTGTAACAGTAAGGCTTGATGGAAAGGACACTCAGGAATCAGAAACACTCGTAGGTGGATGTGACTTCTATGCCTTCCCGCGTCTGGATCTCAAAGGAGAACGACTTGCATGGATTGAATGGTGCCATCCTAACATGCCATGGGATAGGTCTCAACTTTGGGTTGGGTATATATCTGAGAAAGGGGAAGTCTCAAAACGAGTCTGTGTTTCTGGCTCTAATCCTGCAATTAAGGAATCTCCAATTGAACCCAAGTGGTCTGCAAACGGGGAACTATTTTTCATTACTGACAGAAGAAGTGGGTTTTGGAATCTCTATAAATGGGTTGAGTCTACTAATGAGGTCATATCAGTTTATTCTTTGGATGCTGAATTTTCCAAACCATTATGGGTTTTTGGCCTGAATTCTTATGAATTTATTGAGAGCCATGAACATAAGAACCTTATTGCTTGCAGCTATAGGAAAAACGGGAGATCATATCTTGGAATTGTGGATGATGTTCAGGGCTCATTATCCCTGCTTAATATTCCTTTCACGGATATTGATAATATTACTTTGGGGATTAGTTGCCTATATGTGGAAGGAGCATCAGAAGTTCATCCATTGTCTGTTGCTAAGGTGACATTAGATGATCAAAAATCAAAAGCAGTTGAATTCAAGATTATTTGGTCTTCTTCACCCGATTGCTTAAAATATGAGTCCTACTTCAGCCTACCTGAATTAATTGAGTTCCCAACAGAAGTTCCTGGTCAAACTGCCTTTGCATACTTTTATCCACCATCAAATCCTAGCTACCAAGCAACTAAGGACGAAAAGCCTCCACTATTACTGAAAAGCCATGGAGGACCCACAGCTGAAGCACGTGGAATTTTAAATCTAAGTGTTCAGTACTGGACTAGTCGTGGTTGGGCATTCGTTGATGTTAACTATGGTGGAAGCACTGGTTATGGCAGAGAATATCGTGAAAGGCTGTTGAAGAAATGGGGAATTGTGGATGTAAATGATTGTTGCAGCTGTGCTAGTTATCTGGTGGAATCTGGAAAGGTAGATGGGAAACGACTTTGCATTACTGGAGGCTCTGCAGGTGGATACACAACCTTAGCTGCACTTGCATTTCGAGACACCTTCAAGGCTGGAGCTTCCTTGTATGGTGTAGCTGACTTAAACATGTTGAGGGCCGAAACTCACAAATTTGAATCTCATTATATGGATAATCTTGTTGGAAGTGATCAGGATTTCTTTGATAGATCACCAATCAACTTTGTTGATAAATTTTCTTGCCCAATAATCTTATTCCAAGGATTGGAAGACAAGGTTGTACCCCCAGATCAAGCTCGTACAATTTACCGTGCTCTGAAGGAAAAGGGCTTGCCAGTTGCTCTCGTGGAGTATGAAGGAGAACAACATGGTTTCCGCAAGGCTGAGAACATTATATTCACTCTGGAACAGCAAATGGTTTTCTTTGCACGTTTAGTTGGACACTTCAAGGTGGCTGATGAGCTGTGCCAGATATTTGATGGGGATCGACTGTGTGCTACAGGGGAATTTGCTGGTGGATACACTACCTTAGCTGCTCTTGCTTTTAGAAACACCTTCAAGGCAGAAGCTTCCTTGTATGGCGTGAGTGCCTTGCTCTATATTAGGGCTCATTCTGTGGACAGCGATATCAGAAGGAACATTTGGTTGTTTCTGCAAACTTGGACACTATTTAATATGCAAGGGATTAAGACAGATGAACTCTCCACCAGCAGTGATCTCTATGAACGGGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9

Protein Analysis

758

Amino Acids

84.77

Weight (kDa)

5.22

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 461 - 660 2.9e-47 Prolyl oligopeptidase family
BD-FAE PF20434 499 - 619 9.1e-09 BD-FAE
DLH PF01738 509 - 644 6.7e-09 Dienelactone hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012367)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G36210
fragaria_vesca FvH4_2g34950
malus_domestica MD08G1107000.v1.1 MD08G1107200.v1.1 MD15G1087700.v1.1 MD15G1087800.v1.1
prunus_persica Prupe.1G441500_v2.0.a1
pyrus_communis pycom08g08900 pycom08g08910 pycom15g08240
rosa_chinensis RchiOBHm_Chr6g0307051
rosa_laevigata RLG00000010738
rosa_multiflora Rmu_sc0005634.1_g000006
rosa_roxburghii Rroxscaffold_7G00161200
rosa_rugosa Rorug06G0355500
rosa_samantha Rh6AG467000 Rh6CG482500 Rh6DG468800
rosa_wichuraiana Rw6G040710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 321
AarI CACCTGC 1 cut(s) 1574
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 1574
AccB1I GGYRCC 4 cut(s) 92, 113, 278, 624
AccB7I CCANNNNNTGG 2 cut(s) 392, 1804
AccBSI CCGCTC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 833
AccII CGCG 3 cut(s) 245, 474, 583
AccIII TCCGGA 1 cut(s) 170
AciI CCGC 9 cut(s) 75, 103, 117, 136, 179, 245, 273, 581, 1915
AclWI GGATC 3 cut(s) 333, 597, 2031
AcoI YGGCCR 1 cut(s) 133
AcsI RAATTY 9 cut(s) 487, 872, 904, 914, 1154, 1368, 1691, 1772, 2045
AcuI CTGAAG 4 cut(s) 268, 1192, 1377, 1877
AcvI CACGTG 1 cut(s) 1364
AcyI GRCGYC 3 cut(s) 5, 93, 279
AfaI GTAC 5 cut(s) 269, 375, 1391, 1821, 1840
AfiI CCNNNNNNNGG 5 cut(s) 102, 392, 586, 1078, 1804
AflIII ACRYGT 1 cut(s) 1668
AgeI ACCGGT 1 cut(s) 217
AhlI ACTAGT 1 cut(s) 1397
AjnI CCWGG 1 cut(s) 1246
AjuI GAANNNNNNNTTGG 4 cut(s) 790, 822, 2164, 2196
AloI GAACNNNNNNTCC 2 cut(s) 165, 197
Alw21I GWGCWC 1 cut(s) 1855
Alw26I GTCTC 6 cut(s) 91, 159, 196, 657, 697, 1614
AlwI GGATC 3 cut(s) 333, 597, 2031
Ama87I CYCGRG 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 170
AoxI GGCC 3 cut(s) 133, 898, 1677
ApeKI GCWGC 5 cut(s) 480, 954, 1512, 1604, 2072
ApoI RAATTY 9 cut(s) 487, 872, 904, 914, 1154, 1368, 1691, 1772, 2045
AseI ATTAAT 1 cut(s) 1223
AsiGI ACCGGT 1 cut(s) 217
AspLEI GCGC 2 cut(s) 95, 281
AspS9I GGNCC 3 cut(s) 398, 1346, 1677
AsuHPI GGTGA 6 cut(s) 64, 199, 360, 1132, 1170, 1743
AsuII TTCGAA 1 cut(s) 250
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 2 cut(s) 398, 1346
BaeI ACNNNNGTAYC 2 cut(s) 291, 324
BanI GGYRCC 4 cut(s) 92, 113, 278, 624
BanII GRGCYC 2 cut(s) 1010, 2149
BauI CACGAG 1 cut(s) 1883
BbrPI CACGTG 1 cut(s) 1364
BbsI GAAGAC 4 cut(s) 216, 301, 1164, 1815
Bbv12I GWGCWC 1 cut(s) 1855
BbvI GCAGC 5 cut(s) 492, 966, 1524, 1591, 2059
BccI CCATC 6 cut(s) 413, 515, 636, 1288, 1544, 2012
BceAI ACGGC 3 cut(s) 104, 148, 286
BcgI CGANNNNNNTGC 2 cut(s) 103, 137
BciT130I CCWGG 1 cut(s) 1248
BciVI GTATCC 2 cut(s) 1583, 2051
BclI TGATCA 2 cut(s) 1132, 1729
BcoDI GTCTC 6 cut(s) 91, 159, 196, 657, 697, 1614
BcuI ACTAGT 1 cut(s) 1397
BfaI CTAG 3 cut(s) 1290, 1398, 1521
BfmI CTRYAG 3 cut(s) 958, 1581, 2037
BfoI RGCGCY 2 cut(s) 96, 282
BfuAI ACCTGC 1 cut(s) 1574
BfuI GTATCC 2 cut(s) 1583, 2051
BisI GCNGC 7 cut(s) 104, 136, 481, 955, 1513, 1605, 2073
BlsI GCNGC 7 cut(s) 105, 137, 482, 956, 1514, 1606, 2074
BmcAI AGTACT 1 cut(s) 1391
Bme1390I CCNGG 1 cut(s) 1248
Bme18I GGWCC 2 cut(s) 398, 1346
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 3 cut(s) 398, 1346, 1677
BmiI GGNNCC 5 cut(s) 94, 115, 280, 626, 1348
BmrFI CCNGG 1 cut(s) 1248
BmsI GCATC 3 cut(s) 421, 856, 1097
BpiI GAAGAC 4 cut(s) 216, 301, 1164, 1815
BpmI CTGGAG 3 cut(s) 369, 1593, 1656
Bpu10I CCTNAGC 4 cut(s) 261, 1116, 1600, 2068
Bpu14I TTCGAA 1 cut(s) 250
BsaAI YACGTR 1 cut(s) 1364
BsaBI GATNNNNATC 1 cut(s) 2022
BsaHI GRCGYC 3 cut(s) 5, 93, 279
BsaI GGTCTC 2 cut(s) 196, 657
BsaJI CCNNGG 4 cut(s) 130, 641, 1339, 1798
BsaWI WCCGGW 4 cut(s) 170, 196, 205, 217
Bsc4I CCNNNNNNNGG 5 cut(s) 102, 392, 586, 1078, 1804
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 6 cut(s) 371, 401, 1397, 1447, 1576, 1874
Bse8I GATNNNNATC 1 cut(s) 2022
BseAI TCCGGA 1 cut(s) 170
BseBI CCWGG 1 cut(s) 1248
BseDI CCNNGG 4 cut(s) 130, 641, 1339, 1798
BseGI GGATG 6 cut(s) 566, 628, 871, 1000, 1099, 1501
BseJI GATNNNNATC 1 cut(s) 2022
BseLI CCNNNNNNNGG 5 cut(s) 102, 392, 586, 1078, 1804
BseMII CTCAG 4 cut(s) 275, 548, 669, 1914
BseNI ACTGG 6 cut(s) 371, 401, 1397, 1447, 1576, 1874
BseX3I CGGCCG 1 cut(s) 133
BseXI GCAGC 5 cut(s) 492, 966, 1524, 1591, 2059
BsgI GTGCAG 1 cut(s) 1590
Bsh1236I CGCG 3 cut(s) 245, 474, 583
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 3 cut(s) 135, 900, 1679
BshNI GGYRCC 4 cut(s) 92, 113, 278, 624
BshTI ACCGGT 1 cut(s) 217
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 1855
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 5 cut(s) 90, 171, 197, 206, 218
BslI CCNNNNNNNGG 5 cut(s) 102, 392, 586, 1078, 1804
BsmAI GTCTC 6 cut(s) 91, 159, 196, 657, 697, 1614
BsmBI CGTCTC 1 cut(s) 91
BsmI GAATGC 1 cut(s) 1412
BsnI GGCC 3 cut(s) 135, 900, 1679
Bso31I GGTCTC 2 cut(s) 196, 657
BsoBI CYCGRG 1 cut(s) 149
Bsp119I TTCGAA 1 cut(s) 250
Bsp1286I GDGCHC 3 cut(s) 1010, 1855, 2149
Bsp13I TCCGGA 1 cut(s) 170
Bsp143I GATC 9 cut(s) 325, 589, 974, 1132, 1729, 1748, 1828, 2023, 2254
Bsp19I CCATGG 2 cut(s) 641, 1339
Bsp68I TCGCGA 1 cut(s) 474
BspACI CCGC 9 cut(s) 75, 103, 117, 136, 179, 245, 273, 581, 1915
BspANI GGCC 3 cut(s) 135, 900, 1679
BspCNI CTCAG 4 cut(s) 274, 547, 670, 1915
BspEI TCCGGA 1 cut(s) 170
BspFNI CGCG 3 cut(s) 245, 474, 583
BspLI GGNNCC 5 cut(s) 94, 115, 280, 626, 1348
BspMAI CTGCAG 1 cut(s) 1585
BspMI ACCTGC 1 cut(s) 1574
BspPI GGATC 3 cut(s) 333, 597, 2031
BspT104I TTCGAA 1 cut(s) 250
BspT107I GGYRCC 4 cut(s) 92, 113, 278, 624
BspTNI GGTCTC 2 cut(s) 196, 657
BsrBI CCGCTC 1 cut(s) 179
BsrFI RCCGGY 1 cut(s) 217
BsrI ACTGG 6 cut(s) 371, 401, 1397, 1447, 1576, 1874
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 4 cut(s) 130, 641, 1339, 1798
BssMI GATC 9 cut(s) 325, 589, 974, 1132, 1729, 1748, 1828, 2023, 2254
BssNI GRCGYC 3 cut(s) 5, 93, 279
BssSI CACGAG 1 cut(s) 1883
BssT1I CCWWGG 3 cut(s) 641, 1339, 1798
Bst2BI CACGAG 1 cut(s) 1883
Bst2UI CCWGG 1 cut(s) 1248
Bst4CI ACNGT 5 cut(s) 446, 457, 511, 1850, 2031
BstACI GRCGYC 3 cut(s) 5, 93, 279
BstBAI YACGTR 1 cut(s) 1364
BstBI TTCGAA 1 cut(s) 250
BstC8I GCNNGC 2 cut(s) 952, 1871
BstDEI CTNAG 9 cut(s) 261, 534, 678, 1116, 1305, 1379, 1600, 1923, 2068
BstDSI CCRYGG 3 cut(s) 130, 641, 1339
BstF5I GGATG 6 cut(s) 566, 628, 871, 1000, 1099, 1501
BstFNI CGCG 3 cut(s) 245, 474, 583
BstH2I RGCGCY 2 cut(s) 96, 282
BstHHI GCGC 2 cut(s) 95, 281
BstKTI GATC 9 cut(s) 328, 592, 977, 1135, 1732, 1751, 1831, 2026, 2257
BstMAI GTCTC 6 cut(s) 91, 159, 196, 657, 697, 1614
BstMBI GATC 9 cut(s) 325, 589, 974, 1132, 1729, 1748, 1828, 2023, 2254
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 4 cut(s) 285, 480, 1610, 2078
BstNI CCWGG 1 cut(s) 1248
BstNSI RCATGY 2 cut(s) 640, 1672
BstSCI CCNGG 1 cut(s) 1246
BstSFI CTRYAG 3 cut(s) 958, 1581, 2037
BstUI CGCG 3 cut(s) 245, 474, 583
BstV1I GCAGC 5 cut(s) 492, 966, 1524, 1591, 2059
BstV2I GAAGAC 4 cut(s) 216, 301, 1164, 1815
BstX2I RGATCY 1 cut(s) 589
BstYI RGATCY 1 cut(s) 589
BstZI CGGCCG 1 cut(s) 133
BsuI GTATCC 2 cut(s) 1583, 2051
BsuRI GGCC 3 cut(s) 135, 900, 1679
BtgI CCRYGG 3 cut(s) 130, 641, 1339
BtsCI GGATG 6 cut(s) 566, 628, 871, 1000, 1099, 1501
BtsI GCAGTG 1 cut(s) 2257
BtsIMutI CAGTG 3 cut(s) 453, 1440, 2257
BtuMI TCGCGA 1 cut(s) 474
BveI ACCTGC 1 cut(s) 1574
Cac8I GCNNGC 2 cut(s) 952, 1871
CfoI GCGC 2 cut(s) 95, 281
Cfr10I RCCGGY 1 cut(s) 217
Cfr13I GGNCC 3 cut(s) 398, 1346, 1677
CseI GACGC 1 cut(s) 572
Csp6I GTAC 5 cut(s) 268, 374, 1390, 1820, 1839
CspAI ACCGGT 1 cut(s) 217
CviAII CATG 8 cut(s) 484, 612, 637, 642, 929, 1340, 1669, 1907
CviQI GTAC 5 cut(s) 268, 374, 1390, 1820, 1839
DdeI CTNAG 9 cut(s) 261, 534, 678, 1116, 1305, 1379, 1600, 1923, 2068
DinI GGCGCC 2 cut(s) 94, 280
DpnI GATC 9 cut(s) 327, 591, 976, 1134, 1731, 1750, 1830, 2025, 2256
DpnII GATC 9 cut(s) 325, 589, 974, 1132, 1729, 1748, 1828, 2023, 2254
DraI TTTAAA 1 cut(s) 1374
EaeI YGGCCR 1 cut(s) 133
EagI CGGCCG 1 cut(s) 133
EciI GGCGGA 1 cut(s) 288
EclXI CGGCCG 1 cut(s) 133
Eco130I CCWWGG 3 cut(s) 641, 1339, 1798
Eco24I GRGCYC 2 cut(s) 1010, 2149
Eco31I GGTCTC 2 cut(s) 196, 657
Eco32I GATATC 2 cut(s) 226, 2167
Eco47I GGWCC 2 cut(s) 398, 1346
Eco52I CGGCCG 1 cut(s) 133
Eco57I CTGAAG 4 cut(s) 268, 1192, 1377, 1877
Eco72I CACGTG 1 cut(s) 1364
Eco88I CYCGRG 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 1346
EcoRI GAATTC 3 cut(s) 487, 904, 1154
EcoRII CCWGG 1 cut(s) 1246
EcoRV GATATC 2 cut(s) 226, 2167
EcoT14I CCWWGG 3 cut(s) 641, 1339, 1798
EcoT38I GRGCYC 2 cut(s) 1010, 2149
EgeI GGCGCC 2 cut(s) 94, 280
EheI GGCGCC 2 cut(s) 94, 280
ErhI CCWWGG 3 cut(s) 641, 1339, 1798
Esp3I CGTCTC 1 cut(s) 91
FaeI CATG 8 cut(s) 487, 615, 640, 645, 932, 1343, 1672, 1910
FatI CATG 8 cut(s) 483, 611, 636, 641, 928, 1339, 1668, 1906
FauI CCCGC 1 cut(s) 588
FbaI TGATCA 2 cut(s) 1132, 1729
FblI GTMKAC 1 cut(s) 833
Fnu4HI GCNGC 7 cut(s) 104, 136, 481, 955, 1513, 1605, 2073
FokI GGATG 6 cut(s) 573, 615, 878, 1007, 1086, 1508
FriOI GRGCYC 2 cut(s) 1010, 2149
Fsp4HI GCNGC 7 cut(s) 104, 136, 481, 955, 1513, 1605, 2073
FspBI CTAG 3 cut(s) 1290, 1398, 1521
GlaI GCGC 2 cut(s) 94, 280
GluI GCNGC 7 cut(s) 104, 136, 481, 955, 1513, 1605, 2073
GsuI CTGGAG 3 cut(s) 369, 1593, 1656
HaeII RGCGCY 2 cut(s) 96, 282
HaeIII GGCC 3 cut(s) 135, 900, 1679
HapII CCGG 5 cut(s) 90, 171, 197, 206, 218
HgaI GACGC 1 cut(s) 572
HhaI GCGC 2 cut(s) 95, 281
Hin1I GRCGYC 3 cut(s) 5, 93, 279
Hin1II CATG 8 cut(s) 487, 615, 640, 645, 932, 1343, 1672, 1910
Hin6I GCGC 2 cut(s) 93, 279
HinP1I GCGC 2 cut(s) 93, 279
HincII GTYRAC 1 cut(s) 1426
HindII GTYRAC 1 cut(s) 1426
HindIII AAGCTT 1 cut(s) 2106
HpaI GTTAAC 1 cut(s) 1426
HpaII CCGG 5 cut(s) 90, 171, 197, 206, 218
HphI GGTGA 6 cut(s) 64, 199, 360, 1132, 1170, 1743
Hpy166II GTNNAC 4 cut(s) 398, 834, 1426, 2158
Hpy188I TCNGA 7 cut(s) 110, 166, 544, 679, 1093, 1857, 2171
Hpy8I GTNNAC 4 cut(s) 398, 834, 1426, 2158
Hpy99I CGWCG 2 cut(s) 125, 166
HpyAV CCTTC 9 cut(s) 229, 292, 586, 1076, 1636, 1852, 1889, 2104, 2166
HpyCH4III ACNGT 5 cut(s) 446, 457, 511, 1850, 2031
HpyCH4IV ACGT 4 cut(s) 5, 436, 1363, 1969
HpyF10VI GCNNNNNNNGC 4 cut(s) 285, 480, 1610, 2078
HpyF3I CTNAG 9 cut(s) 261, 534, 678, 1116, 1305, 1379, 1600, 1923, 2068
HpySE526I ACGT 4 cut(s) 5, 436, 1363, 1969
Hsp92I GRCGYC 3 cut(s) 5, 93, 279
Hsp92II CATG 8 cut(s) 487, 615, 640, 645, 932, 1343, 1672, 1910
HspAI GCGC 2 cut(s) 93, 279
KasI GGCGCC 2 cut(s) 92, 278
Kpn2I TCCGGA 1 cut(s) 170
Ksp22I TGATCA 2 cut(s) 1132, 1729
KspAI GTTAAC 1 cut(s) 1426
Kzo9I GATC 9 cut(s) 325, 589, 974, 1132, 1729, 1748, 1828, 2023, 2254
LmnI GCTCC 4 cut(s) 49, 359, 1085, 1637
Lsp1109I GCAGC 5 cut(s) 492, 966, 1524, 1591, 2059
LweI GCATC 3 cut(s) 421, 856, 1097
MaeI CTAG 3 cut(s) 1290, 1398, 1521
MaeII ACGT 4 cut(s) 5, 436, 1363, 1969
MaeIII GTNAC 3 cut(s) 505, 563, 1120
MalI GATC 9 cut(s) 327, 591, 976, 1134, 1731, 1750, 1830, 2025, 2256
MbiI CCGCTC 1 cut(s) 179
MboI GATC 9 cut(s) 325, 589, 974, 1132, 1729, 1748, 1828, 2023, 2254
MfeI CAATTG 2 cut(s) 501, 744
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 3 cut(s) 1010, 1855, 2149
Mly113I GGCGCC 2 cut(s) 93, 279
MlyI GAGTC 4 cut(s) 161, 711, 839, 1208
MmeI TCCRAC 3 cut(s) 144, 1702, 1957
MroI TCCGGA 1 cut(s) 170
MspA1I CMGCKG 2 cut(s) 1355, 1515
MspI CCGG 5 cut(s) 90, 171, 197, 206, 218
MspR9I CCNGG 1 cut(s) 1248
MunI CAATTG 2 cut(s) 501, 744
Mva1269I GAATGC 1 cut(s) 1412
MvaI CCWGG 1 cut(s) 1248
MvnI CGCG 3 cut(s) 245, 474, 583
MwoI GCNNNNNNNGC 4 cut(s) 285, 480, 1610, 2078
NarI GGCGCC 2 cut(s) 93, 279
NcoI CCATGG 2 cut(s) 641, 1339
NdeII GATC 9 cut(s) 325, 589, 974, 1132, 1729, 1748, 1828, 2023, 2254
NlaIII CATG 8 cut(s) 487, 615, 640, 645, 932, 1343, 1672, 1910
NlaIV GGNNCC 5 cut(s) 94, 115, 280, 626, 1348
NmuCI GTSAC 2 cut(s) 563, 1120
NruI TCGCGA 1 cut(s) 474
NspI RCATGY 2 cut(s) 640, 1672
NspV TTCGAA 1 cut(s) 250
PaeR7I CTCGAG 1 cut(s) 149
PaqCI CACCTGC 1 cut(s) 1574
PciI ACATGT 1 cut(s) 1668
PcsI WCGNNNNNNNCGW 1 cut(s) 442
PctI GAATGC 1 cut(s) 1412
PfeI GAWTC 8 cut(s) 125, 167, 362, 539, 737, 811, 1535, 1697
PflMI CCANNNNNTGG 2 cut(s) 392, 1804
PinAI ACCGGT 1 cut(s) 217
PkrI GCNGC 7 cut(s) 105, 137, 482, 956, 1514, 1606, 2074
PleI GAGTC 4 cut(s) 160, 710, 838, 1207
PluTI GGCGCC 2 cut(s) 96, 282
PmaCI CACGTG 1 cut(s) 1364
PmlI CACGTG 1 cut(s) 1364
PpsI GAGTC 4 cut(s) 160, 710, 838, 1207
Ppu21I YACGTR 1 cut(s) 1364
PpuMI RGGWCCY 1 cut(s) 1346
PscI ACATGT 1 cut(s) 1668
PshBI ATTAAT 1 cut(s) 1223
PsiI TTATAA 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 1346
Psp6I CCWGG 1 cut(s) 1246
PspCI CACGTG 1 cut(s) 1364
PspGI CCWGG 1 cut(s) 1246
PspN4I GGNNCC 5 cut(s) 94, 115, 280, 626, 1348
PspPI GGNCC 3 cut(s) 398, 1346, 1677
PspPPI RGGWCCY 1 cut(s) 1346
PspXI VCTCGAGB 1 cut(s) 149
PstI CTGCAG 1 cut(s) 1585
PsuI RGATCY 1 cut(s) 589
PvuII CAGCTG 2 cut(s) 1355, 1515
RruI TCGCGA 1 cut(s) 474
RsaI GTAC 5 cut(s) 269, 375, 1391, 1821, 1840
RsaNI GTAC 5 cut(s) 268, 374, 1390, 1820, 1839
SatI GCNGC 7 cut(s) 104, 136, 481, 955, 1513, 1605, 2073
Sau3AI GATC 9 cut(s) 325, 589, 974, 1132, 1729, 1748, 1828, 2023, 2254
Sau96I GGNCC 3 cut(s) 398, 1346, 1677
ScaI AGTACT 1 cut(s) 1391
SchI GAGTC 4 cut(s) 161, 711, 839, 1208
ScrFI CCNGG 1 cut(s) 1248
SduI GDGCHC 3 cut(s) 1010, 1855, 2149
SfaNI GCATC 3 cut(s) 421, 856, 1097
SfcI CTRYAG 3 cut(s) 958, 1581, 2037
SfoI GGCGCC 2 cut(s) 94, 280
Sfr274I CTCGAG 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 250
SinI GGWCC 2 cut(s) 398, 1346
SlaI CTCGAG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SpeI ACTAGT 1 cut(s) 1397
SsiI CCGC 9 cut(s) 75, 103, 117, 136, 179, 245, 273, 581, 1915
SspDI GGCGCC 2 cut(s) 92, 278
SspI AATATT 2 cut(s) 1027, 1051
SspMI CTAG 3 cut(s) 1290, 1398, 1521
StyD4I CCNGG 1 cut(s) 1246
StyI CCWWGG 3 cut(s) 641, 1339, 1798
TaaI ACNGT 5 cut(s) 446, 457, 511, 1850, 2031
TaiI ACGT 4 cut(s) 8, 439, 1366, 1972
TaqI TCGA 5 cut(s) 123, 150, 250, 1618, 2026
TatI WGTACW 1 cut(s) 1389
TauI GCSGC 2 cut(s) 106, 138
TfiI GAWTC 8 cut(s) 125, 167, 362, 539, 737, 811, 1535, 1697
TscAI CASTG 3 cut(s) 460, 1447, 2257
TseFI GTSAC 2 cut(s) 563, 1120
TseI GCWGC 5 cut(s) 480, 954, 1512, 1604, 2072
Tsp45I GTSAC 2 cut(s) 563, 1120
TspDTI ATGAA 8 cut(s) 500, 772, 927, 945, 1088, 1908, 2250, 2277
TspGWI ACGGA 2 cut(s) 150, 1052
TspRI CASTG 3 cut(s) 460, 1447, 2257
Van91I CCANNNNNTGG 2 cut(s) 392, 1804
VpaK11BI GGWCC 2 cut(s) 398, 1346
VspI ATTAAT 1 cut(s) 1223
XapI RAATTY 9 cut(s) 487, 872, 904, 914, 1154, 1368, 1691, 1772, 2045
XceI RCATGY 2 cut(s) 640, 1672
XcmI CCANNNNNNNNNTGG 1 cut(s) 2015
XhoI CTCGAG 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 833
XspI CTAG 3 cut(s) 1290, 1398, 1521
ZraI GACGTC 1 cut(s) 6
ZrmI AGTACT 1 cut(s) 1391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.