FvH4_2g35150

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006181
Physical Location & Seq
Forward (+)
25974062 .. 25976558
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35150.t6

Sequence Viewer

Length: 600 bp
ATGTGTCAGATACGAGTTGGTAATGTTTTGATCACTACTCTCTACATTGTCTGGATTGGTATTAGCGGTTCGAGTTTGAGCTATGGAGATTTGGTAGATAAGAAGGTTCTGAATGTAGGGGAAGAGCTGTGGAGGGAGATTCTGCCGCTACGAATGGGTTCGCGGCTTTACCGGCTTGAAGGGATCAAGCCGAGTACTTGGTATGAAGTGAAGATATCATATCCAGCTTCTATTCCGGCTAGCTTTTCCATACAACTCAAGAGAGGGGACTTGGAGTTACCACTGAATGGGAACAGAAGATTACTGAACACCGAAAAGCTGATTTTCAAGAGCGAAAATGATGATCAGAACGATCAGGGTGACATGTATGTGTTGGTGACTGTGGAGCCTGAGGGGGTTGTTGCCATACCAAATGTGCAGGAGAGGGAGCACATCATCTTCAACATAGTTTGTGATGAACTGTTGCTGGGGATTCCTCACCAAGCTTGGTGGGTCGTAGGCTTTGTGGTGGTGTGTTTGGTTTTGGCCATCATCATACCCTCATTTCTTCCACCCTTTTTGTTAAGAGCAGTTGATCAGAATGCTTCGAAAAATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.45

Weight (kDa)

5.25

Isoelectric Point (pI)

28.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 287
AccII CGCG 1 cut(s) 163
AciI CCGC 3 cut(s) 66, 146, 163
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 525
AcsI RAATTY 1 cut(s) 592
AfaI GTAC 1 cut(s) 196
AfiI CCNNNNNNNGG 1 cut(s) 287
AflIII ACRYGT 1 cut(s) 363
AgsI TTSAA 3 cut(s) 179, 328, 442
AluBI AGCT 6 cut(s) 81, 127, 227, 243, 319, 485
AluI AGCT 6 cut(s) 81, 127, 227, 243, 319, 485
Alw21I GWGCWC 1 cut(s) 432
AlwI GGATC 1 cut(s) 191
AoxI GGCC 1 cut(s) 525
ApoI RAATTY 1 cut(s) 592
Asp700I GAANNNNTTC 1 cut(s) 157
AsuHPI GGTGA 3 cut(s) 371, 388, 470
AsuII TTCGAA 1 cut(s) 587
AsuNHI GCTAGC 1 cut(s) 239
AxyI CCTNAGG 1 cut(s) 390
BalI TGGCCA 1 cut(s) 527
Bbv12I GWGCWC 1 cut(s) 432
BccI CCATC 1 cut(s) 536
BclI TGATCA 3 cut(s) 30, 343, 574
BfaI CTAG 1 cut(s) 240
BisI GCNGC 2 cut(s) 146, 164
BlsI GCNGC 2 cut(s) 147, 165
BmcAI AGTACT 1 cut(s) 196
BmiI GGNNCC 1 cut(s) 387
BmtI GCTAGC 1 cut(s) 243
Bpu14I TTCGAA 1 cut(s) 587
BpuEI CTTGAG 1 cut(s) 242
BsaXI ACNNNNNCTCC 2 cut(s) 78, 108
Bsc4I CCNNNNNNNGG 1 cut(s) 287
Bse118I RCCGGY 1 cut(s) 171
Bse21I CCTNAGG 1 cut(s) 390
BseLI CCNNNNNNNGG 1 cut(s) 287
BseMII CTCAG 1 cut(s) 381
BseYI CCCAGC 1 cut(s) 466
BsgI GTGCAG 1 cut(s) 437
Bsh1236I CGCG 1 cut(s) 163
BshFI GGCC 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 432
BsiSI CCGG 2 cut(s) 172, 236
BslFI GGGAC 1 cut(s) 281
BslI CCNNNNNNNGG 1 cut(s) 287
BsmFI GGGAC 1 cut(s) 281
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 1 cut(s) 527
Bsp119I TTCGAA 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 5 cut(s) 30, 183, 343, 352, 574
BspACI CCGC 3 cut(s) 66, 146, 163
BspANI GGCC 1 cut(s) 527
BspCNI CTCAG 1 cut(s) 382
BspFNI CGCG 1 cut(s) 163
BspLI GGNNCC 1 cut(s) 387
BspOI GCTAGC 1 cut(s) 243
BspPI GGATC 1 cut(s) 191
BspQI GCTCTTC 1 cut(s) 117
BspT104I TTCGAA 1 cut(s) 587
BsrFI RCCGGY 1 cut(s) 171
BssAI RCCGGY 1 cut(s) 171
BssMI GATC 5 cut(s) 30, 183, 343, 352, 574
Bst4CI ACNGT 2 cut(s) 382, 462
Bst6I CTCTTC 1 cut(s) 117
BstBI TTCGAA 1 cut(s) 587
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 1 cut(s) 390
BstFNI CGCG 1 cut(s) 163
BstKTI GATC 5 cut(s) 33, 186, 346, 355, 577
BstMBI GATC 5 cut(s) 30, 183, 343, 352, 574
BstMWI GCNNNNNNNGC 1 cut(s) 172
BstNSI RCATGY 1 cut(s) 367
BstUI CGCG 1 cut(s) 163
Bsu36I CCTNAGG 1 cut(s) 390
BsuRI GGCC 1 cut(s) 527
BtsIMutI CAGTG 1 cut(s) 281
Cac8I GCNNGC 1 cut(s) 241
Cfr10I RCCGGY 1 cut(s) 171
Csp6I GTAC 1 cut(s) 195
CspCI CAANNNNNGTGG 4 cut(s) 470, 505, 540, 575
CviAII CATG 1 cut(s) 364
CviQI GTAC 1 cut(s) 195
DdeI CTNAG 1 cut(s) 390
DpnI GATC 5 cut(s) 32, 185, 345, 354, 576
DpnII GATC 5 cut(s) 30, 183, 343, 352, 574
EaeI YGGCCR 1 cut(s) 525
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Eco32I GATATC 1 cut(s) 216
Eco81I CCTNAGG 1 cut(s) 390
EcoRV GATATC 1 cut(s) 216
FaeI CATG 1 cut(s) 367
FaiI YATR 9 cut(s) 84, 204, 220, 251, 365, 369, 407, 446, 536
FaqI GGGAC 1 cut(s) 281
FatI CATG 1 cut(s) 363
FbaI TGATCA 3 cut(s) 30, 343, 574
Fnu4HI GCNGC 2 cut(s) 146, 164
Fsp4HI GCNGC 2 cut(s) 146, 164
FspBI CTAG 1 cut(s) 240
GluI GCNGC 2 cut(s) 146, 164
GsaI CCCAGC 1 cut(s) 470
HaeIII GGCC 1 cut(s) 527
HapII CCGG 2 cut(s) 172, 236
Hin1II CATG 1 cut(s) 367
HindIII AAGCTT 1 cut(s) 483
HinfI GANTC 2 cut(s) 139, 472
HpaII CCGG 2 cut(s) 172, 236
HphI GGTGA 3 cut(s) 371, 388, 470
Hpy188I TCNGA 4 cut(s) 9, 111, 348, 579
Hpy188III TCNNGA 4 cut(s) 52, 259, 328, 597
HpyAV CCTTC 2 cut(s) 97, 173
HpyCH4III ACNGT 2 cut(s) 382, 462
HpyCH4V TGCA 1 cut(s) 418
HpyF10VI GCNNNNNNNGC 1 cut(s) 172
HpyF3I CTNAG 1 cut(s) 390
Hsp92II CATG 1 cut(s) 367
Ksp22I TGATCA 3 cut(s) 30, 343, 574
Kzo9I GATC 5 cut(s) 30, 183, 343, 352, 574
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 2 cut(s) 385, 427
LpnPI CCDG 8 cut(s) 37, 185, 237, 249, 341, 402, 404, 452
MaeI CTAG 1 cut(s) 240
MaeIII GTNAC 3 cut(s) 276, 359, 376
MalI GATC 5 cut(s) 32, 185, 345, 354, 576
MboI GATC 5 cut(s) 30, 183, 343, 352, 574
MboII GAAGA 5 cut(s) 134, 223, 309, 430, 539
MhlI GDGCHC 1 cut(s) 432
MlsI TGGCCA 1 cut(s) 527
MluCI AATT 1 cut(s) 592
MluNI TGGCCA 1 cut(s) 527
MnlI CCTC 6 cut(s) 126, 257, 385, 417, 486, 550
Mox20I TGGCCA 1 cut(s) 527
MroXI GAANNNNTTC 1 cut(s) 157
MscI TGGCCA 1 cut(s) 527
MseI TTAA 1 cut(s) 563
MslI CAYNNNNRTG 1 cut(s) 368
Msp20I TGGCCA 1 cut(s) 527
MspI CCGG 2 cut(s) 172, 236
Mva1269I GAATGC 1 cut(s) 586
MvnI CGCG 1 cut(s) 163
MwoI GCNNNNNNNGC 1 cut(s) 172
NdeII GATC 5 cut(s) 30, 183, 343, 352, 574
NheI GCTAGC 1 cut(s) 239
NlaIII CATG 1 cut(s) 367
NlaIV GGNNCC 1 cut(s) 387
NmeAIII GCCGAG 1 cut(s) 216
NmuCI GTSAC 2 cut(s) 359, 376
NspI RCATGY 1 cut(s) 367
NspV TTCGAA 1 cut(s) 587
PciI ACATGT 1 cut(s) 363
PciSI GCTCTTC 1 cut(s) 117
PctI GAATGC 1 cut(s) 586
PdmI GAANNNNTTC 1 cut(s) 157
PfeI GAWTC 2 cut(s) 139, 472
PflMI CCANNNNNTGG 1 cut(s) 287
PkrI GCNGC 2 cut(s) 147, 165
PscI ACATGT 1 cut(s) 363
PspFI CCCAGC 1 cut(s) 466
PspN4I GGNNCC 1 cut(s) 387
PsrI GAACNNNNNNTAC 2 cut(s) 52, 84
RsaI GTAC 1 cut(s) 196
RsaNI GTAC 1 cut(s) 195
RseI CAYNNNNRTG 1 cut(s) 368
SapI GCTCTTC 1 cut(s) 117
SaqAI TTAA 1 cut(s) 563
SatI GCNGC 2 cut(s) 146, 164
Sau3AI GATC 5 cut(s) 30, 183, 343, 352, 574
ScaI AGTACT 1 cut(s) 196
SduI GDGCHC 1 cut(s) 432
SetI ASST 7 cut(s) 83, 108, 129, 229, 245, 321, 487
SfuI TTCGAA 1 cut(s) 587
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 1 cut(s) 592
SsiI CCGC 3 cut(s) 66, 146, 163
SspMI CTAG 1 cut(s) 240
TaaI ACNGT 2 cut(s) 382, 462
TaqI TCGA 2 cut(s) 71, 587
TasI AATT 1 cut(s) 592
TatI WGTACW 1 cut(s) 194
TauI GCSGC 2 cut(s) 148, 166
TfiI GAWTC 2 cut(s) 139, 472
Tru1I TTAA 1 cut(s) 563
Tru9I TTAA 1 cut(s) 563
TscAI CASTG 1 cut(s) 288
TseFI GTSAC 2 cut(s) 359, 376
Tsp45I GTSAC 2 cut(s) 359, 376
TspDTI ATGAA 2 cut(s) 219, 471
TspRI CASTG 1 cut(s) 288
Van91I CCANNNNNTGG 1 cut(s) 287
XapI RAATTY 1 cut(s) 592
XceI RCATGY 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 157
XspI CTAG 1 cut(s) 240
ZrmI AGTACT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.