MD08G1109000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006181
Physical Location & Seq
Forward (+)
9591394 .. 9593425
2032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1109000.v1.1.491

Sequence Viewer

Length: 591 bp
ATGCGTCGAATTCGTAATCTTTTGATCAGCCTCTGCATTATCTGCATCATCATCAGCTGCTCAAATTCAAGCGAGGCAAACGCGACAGACATCAAAGTTTTGAAGGTTGGTGAAGAGCTGTGGAGGGAGACTCTACCGCTGCATTCGGGTTCATGCCTTTACCGGCTTCATGGACTCAAGCCTTATACTTCGTATGAAGTGAAGATATCATATCCTGCTTCTATACCGGCTAGCTTTTCCTTGCAACTCAAGAAAGGGGACTTGGAATCGGCGCTGACTGGGAACCGAAGATTACTAAATACGGAAAAGCTTATTTTCAAGACTGAGAGTCTTGGCTCTGATGATCAGGGTGAAATGTCTGTGTTGGTGAGTGTGGAGCCTGAGGGGGTTGTTGCAATACCAAATGTGCAAGAGAGGGAACACATCATATTCAACATAGTTTGTGATGAACTACTATTGGGGATTCCGTACAAAGCTTGGTGGGTTGTAGGCTTTGTAGTGGTGTGTTTGGCCTTGGCGTTCACCGTACCGTCATTCCTTCCACCGTTTTTGCTGCCGCGGGTGGATGAGAATGCTTCTAAAGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

197

Amino Acids

21.79

Weight (kDa)

5.67

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 83, 559
AciI CCGC 3 cut(s) 137, 557, 559
AcsI RAATTY 2 cut(s) 9, 64
AfaI GTAC 2 cut(s) 470, 528
AgsI TTSAA 4 cut(s) 69, 103, 319, 433
AhdI GACNNNNNGTC 1 cut(s) 327
AjuI GAANNNNNNNTTGG 2 cut(s) 245, 277
AluBI AGCT 5 cut(s) 57, 118, 234, 310, 476
AluI AGCT 5 cut(s) 57, 118, 234, 310, 476
Alw26I GTCTC 1 cut(s) 122
AlwNI CAGNNNCTG 1 cut(s) 33
AoxI GGCC 1 cut(s) 510
ApeKI GCWGC 3 cut(s) 57, 139, 553
ApoI RAATTY 2 cut(s) 9, 64
AspLEI GCGC 1 cut(s) 274
AsuHPI GGTGA 4 cut(s) 122, 362, 379, 514
AsuNHI GCTAGC 1 cut(s) 230
AxyI CCTNAGG 1 cut(s) 381
BbvI GCAGC 3 cut(s) 44, 126, 540
BclI TGATCA 2 cut(s) 24, 343
BcoDI GTCTC 1 cut(s) 122
BfaI CTAG 1 cut(s) 231
BfoI RGCGCY 1 cut(s) 275
BisI GCNGC 4 cut(s) 58, 140, 554, 557
BlsI GCNGC 4 cut(s) 59, 141, 555, 558
BmeRI GACNNNNNGTC 1 cut(s) 327
BmiI GGNNCC 2 cut(s) 284, 378
BmrI ACTGGG 1 cut(s) 288
BmsI GCATC 1 cut(s) 54
BmtI GCTAGC 1 cut(s) 234
BmuI ACTGGG 1 cut(s) 288
BplI GAGNNNNNCTC 2 cut(s) 115, 147
BpuEI CTTGAG 2 cut(s) 161, 233
BsaJI CCNNGG 2 cut(s) 513, 557
Bse118I RCCGGY 2 cut(s) 162, 226
Bse1I ACTGG 1 cut(s) 283
Bse21I CCTNAGG 1 cut(s) 381
BseDI CCNNGG 2 cut(s) 513, 557
BseGI GGATG 1 cut(s) 571
BseMII CTCAG 2 cut(s) 315, 372
BseNI ACTGG 1 cut(s) 283
BseXI GCAGC 3 cut(s) 44, 126, 540
Bsh1236I CGCG 2 cut(s) 83, 559
BshFI GGCC 1 cut(s) 512
BsiSI CCGG 2 cut(s) 163, 227
BslFI GGGAC 1 cut(s) 272
BsmAI GTCTC 1 cut(s) 122
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 2 cut(s) 142, 577
BsnI GGCC 1 cut(s) 512
Bsp143I GATC 2 cut(s) 24, 343
BspACI CCGC 3 cut(s) 137, 557, 559
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 2 cut(s) 316, 373
BspFNI CGCG 2 cut(s) 83, 559
BspLI GGNNCC 2 cut(s) 284, 378
BspOI GCTAGC 1 cut(s) 234
BspQI GCTCTTC 1 cut(s) 108
BsrFI RCCGGY 2 cut(s) 162, 226
BsrI ACTGG 1 cut(s) 283
BssAI RCCGGY 2 cut(s) 162, 226
BssECI CCNNGG 2 cut(s) 513, 557
BssMI GATC 2 cut(s) 24, 343
BssT1I CCWWGG 1 cut(s) 513
Bst4CI ACNGT 3 cut(s) 526, 531, 546
Bst6I CTCTTC 1 cut(s) 108
BstAPI GCANNNNNTGC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 232
BstDEI CTNAG 2 cut(s) 324, 381
BstDSI CCRYGG 1 cut(s) 557
BstF5I GGATG 1 cut(s) 571
BstFNI CGCG 2 cut(s) 83, 559
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 2 cut(s) 27, 346
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 2 cut(s) 24, 343
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstUI CGCG 2 cut(s) 83, 559
BstV1I GCAGC 3 cut(s) 44, 126, 540
Bsu36I CCTNAGG 1 cut(s) 381
BsuRI GGCC 1 cut(s) 512
BtgI CCRYGG 1 cut(s) 557
BtsCI GGATG 1 cut(s) 571
Cac8I GCNNGC 1 cut(s) 232
CaiI CAGNNNCTG 1 cut(s) 33
CfoI GCGC 1 cut(s) 274
Cfr10I RCCGGY 2 cut(s) 162, 226
Cfr42I CCGCGG 1 cut(s) 560
Csp6I GTAC 2 cut(s) 469, 527
CspCI CAANNNNNGTGG 2 cut(s) 531, 566
CviAII CATG 2 cut(s) 153, 170
CviQI GTAC 2 cut(s) 469, 527
DdeI CTNAG 2 cut(s) 324, 381
DpnI GATC 2 cut(s) 26, 345
DpnII GATC 2 cut(s) 24, 343
DriI GACNNNNNGTC 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 108
Eam1105I GACNNNNNGTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 513
Eco32I GATATC 1 cut(s) 207
Eco81I CCTNAGG 1 cut(s) 381
EcoRI GAATTC 1 cut(s) 9
EcoRV GATATC 1 cut(s) 207
EcoT14I CCWWGG 1 cut(s) 513
ErhI CCWWGG 1 cut(s) 513
FaeI CATG 2 cut(s) 156, 173
FaiI YATR 8 cut(s) 154, 171, 186, 195, 211, 224, 428, 437
FaqI GGGAC 1 cut(s) 272
FatI CATG 2 cut(s) 152, 169
FauI CCCGC 1 cut(s) 552
FbaI TGATCA 2 cut(s) 24, 343
Fnu4HI GCNGC 4 cut(s) 58, 140, 554, 557
FokI GGATG 1 cut(s) 578
Fsp4HI GCNGC 4 cut(s) 58, 140, 554, 557
FspBI CTAG 1 cut(s) 231
GlaI GCGC 1 cut(s) 273
GluI GCNGC 4 cut(s) 58, 140, 554, 557
HaeII RGCGCY 1 cut(s) 275
HaeIII GGCC 1 cut(s) 512
HapII CCGG 2 cut(s) 163, 227
HhaI GCGC 1 cut(s) 274
Hin1II CATG 2 cut(s) 156, 173
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HindIII AAGCTT 2 cut(s) 308, 474
HinfI GANTC 6 cut(s) 130, 174, 266, 328, 463, 584
HpaII CCGG 2 cut(s) 163, 227
HphI GGTGA 4 cut(s) 122, 362, 379, 514
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 1 cut(s) 340
Hpy188III TCNNGA 3 cut(s) 250, 319, 588
Hpy8I GTNNAC 1 cut(s) 522
Hpy99I CGWCG 1 cut(s) 9
HpyAV CCTTC 2 cut(s) 97, 548
HpyCH4III ACNGT 3 cut(s) 526, 531, 546
HpyCH4V TGCA 6 cut(s) 36, 45, 142, 244, 395, 409
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpyF3I CTNAG 2 cut(s) 324, 381
Hsp92II CATG 2 cut(s) 156, 173
HspAI GCGC 1 cut(s) 272
Ksp22I TGATCA 2 cut(s) 24, 343
KspI CCGCGG 1 cut(s) 560
Kzo9I GATC 2 cut(s) 24, 343
LguI GCTCTTC 1 cut(s) 108
LmnI GCTCC 1 cut(s) 376
LpnPI CCDG 6 cut(s) 176, 228, 240, 264, 332, 393
Lsp1109I GCAGC 3 cut(s) 44, 126, 540
LweI GCATC 1 cut(s) 54
MaeI CTAG 1 cut(s) 231
MalI GATC 2 cut(s) 26, 345
MboI GATC 2 cut(s) 24, 343
MboII GAAGA 3 cut(s) 125, 214, 300
MluCI AATT 2 cut(s) 9, 64
MlyI GAGTC 3 cut(s) 124, 168, 337
MnlI CCTC 5 cut(s) 41, 67, 117, 376, 408
MspA1I CMGCKG 3 cut(s) 57, 139, 559
MspI CCGG 2 cut(s) 163, 227
Mva1269I GAATGC 2 cut(s) 142, 577
MvnI CGCG 2 cut(s) 83, 559
MwoI GCNNNNNNNGC 1 cut(s) 42
NdeII GATC 2 cut(s) 24, 343
NheI GCTAGC 1 cut(s) 230
NlaIII CATG 2 cut(s) 156, 173
NlaIV GGNNCC 2 cut(s) 284, 378
PciSI GCTCTTC 1 cut(s) 108
PctI GAATGC 2 cut(s) 142, 577
PfeI GAWTC 3 cut(s) 266, 463, 584
PkrI GCNGC 4 cut(s) 59, 141, 555, 558
PleI GAGTC 3 cut(s) 124, 168, 336
PpsI GAGTC 3 cut(s) 124, 168, 336
PspN4I GGNNCC 2 cut(s) 284, 378
PstNI CAGNNNCTG 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 57
RsaI GTAC 2 cut(s) 470, 528
RsaNI GTAC 2 cut(s) 469, 527
SacII CCGCGG 1 cut(s) 560
SapI GCTCTTC 1 cut(s) 108
SatI GCNGC 4 cut(s) 58, 140, 554, 557
Sau3AI GATC 2 cut(s) 24, 343
SchI GAGTC 3 cut(s) 124, 168, 337
SetI ASST 6 cut(s) 59, 108, 120, 236, 312, 478
SfaNI GCATC 1 cut(s) 54
Sfr303I CCGCGG 1 cut(s) 560
SgrBI CCGCGG 1 cut(s) 560
SmlI CTYRAG 2 cut(s) 176, 248
SmoI CTYRAG 2 cut(s) 176, 248
Sse9I AATT 2 cut(s) 9, 64
SsiI CCGC 3 cut(s) 137, 557, 559
SspMI CTAG 1 cut(s) 231
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 3 cut(s) 526, 531, 546
TaqI TCGA 1 cut(s) 7
TasI AATT 2 cut(s) 9, 64
TauI GCSGC 1 cut(s) 559
TfiI GAWTC 3 cut(s) 266, 463, 584
TseI GCWGC 3 cut(s) 57, 139, 553
TspDTI ATGAA 4 cut(s) 141, 158, 210, 462
TspGWI ACGGA 2 cut(s) 317, 456
XapI RAATTY 2 cut(s) 9, 64
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.