Rorug06G0353100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0006181
Physical Location & Seq
Reverse (-)
50186928 .. 50190467
3540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0353100.1

Sequence Viewer

Length: 1056 bp
ATGGTGAATATCAAGAACTTATGGTATAAGTATCAATTTGTGAAGGCTTACATAAGGAATTCCAGAGGAGTGCAGCTTTTCACTTGCAGATGGTTGCCTTTCTCTTCCCCAAAGGCCCTTGTTTTCCTTTGCCATGGTTACGGCATGGAGTGCAGCGGTTTCATGAAAGAATGCGGGGTCCGGCTAGCTAATGCCGGGTATGCGGTGTTCGGAATCGATTACGAGGGCCACGGACGTTCTCGGGGGGCAAGGTGTTACATTAAGAAGTTCGAAAACATCGTTAACGATTGCAATGACTTCTTCAAGTCGATTTGTGCGGAGGAAGAGTACAGGGACAAGTGTAGGTTCTTGTATGGAGAATCCATGGGAGGGGCAGTGGCTTTATTGCTTCATAAGAAGGACCCTAAATTTTGGAATGGTGCTGTTCTTGTTGCACCAATGTGTAAGATATCAGAGAAAGTAAAGCCGCATCCACTGGTTGTGAATGTATTGACTAGAGTGGAGGAGATTATACCGAAATGGAAGATTGTACCCACCAAAGACGTCATTGATTCAGCCTTCAAGGACCCTGTAAAGCGCGAAGTGATAAGAAGCAACAAGCTTATCTACCAAGACAAGCCGAGGCTGAAAACAGCTTTGGAAATGCTTAGAACTAGCATGAGCCTTGAAGACAGTTTACATGAGGTGAAACTGCCTTTCTTTGTTTTACACGGGGAGGCAGATACAGTTACAGACCCTGAAGTAAGCAAGTCTCTGTACGAAAAAGCCAGCAGCCCAGACAAGACCATAAAATTGTACCCTGGAATGTGGCATGGCTTAACATCAGGAGAGCCTGATGGGAACGTCGAAATCGTGTTTGCAGACATCACAGTTTGGCTTGAGAAGCACACTACAGACAGCAATGTCATGGTTGTGCAGCCAATTCAAACATTTAATGACGGCATCGTGAAGTTGAACACCACAGCATCATCCCCCGAAACCATGAACAAGCGGCAGAAAGGGAGCTATTTTTGTGGGTTAAAGGGACGAAGATTACAGCATCACTCAGCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

39.94

Weight (kDa)

9.05

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 36 - 278 1.5e-65 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 40 - 154 1.3e-09 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 41 - 273 5.6e-09 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 902
AatII GACGTC 1 cut(s) 546
AccII CGCG 1 cut(s) 579
AciI CCGC 6 cut(s) 156, 174, 203, 317, 467, 991
AcsI RAATTY 2 cut(s) 58, 407
AcuI CTGAAG 1 cut(s) 759
AcyI GRCGYC 1 cut(s) 543
AfaI GTAC 4 cut(s) 329, 531, 758, 797
AfiI CCNNNNNNNGG 1 cut(s) 369
AgsI TTSAA 5 cut(s) 304, 562, 668, 926, 955
AjnI CCWGG 1 cut(s) 799
AjuI GAANNNNNNNTTGG 2 cut(s) 620, 652
AleI CACNNNNGTG 1 cut(s) 439
AluBI AGCT 5 cut(s) 76, 188, 601, 635, 1005
AluI AGCT 5 cut(s) 76, 188, 601, 635, 1005
Alw26I GTCTC 1 cut(s) 756
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 240
AoxI GGCC 2 cut(s) 114, 226
ApeKI GCWGC 4 cut(s) 73, 153, 771, 916
ApoI RAATTY 2 cut(s) 58, 407
AspLEI GCGC 1 cut(s) 579
AspS9I GGNCC 5 cut(s) 115, 178, 226, 400, 565
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 2 cut(s) 16, 697
AsuII TTCGAA 1 cut(s) 270
AsuNHI GCTAGC 1 cut(s) 184
AvaI CYCGRG 1 cut(s) 240
AvaII GGWCC 3 cut(s) 178, 400, 565
BarI GAAGNNNNNNTAC 2 cut(s) 660, 692
BbsI GAAGAC 1 cut(s) 675
BbvI GCAGC 4 cut(s) 85, 165, 783, 928
BccI CCATC 2 cut(s) 84, 830
BceAI ACGGC 2 cut(s) 157, 955
BciT130I CCWGG 1 cut(s) 801
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 1 cut(s) 756
BfaI CTAG 3 cut(s) 185, 495, 654
BfmI CTRYAG 1 cut(s) 891
BisI GCNGC 6 cut(s) 74, 154, 467, 772, 917, 992
BlsI GCNGC 6 cut(s) 75, 155, 468, 773, 918, 993
Bme1390I CCNGG 2 cut(s) 196, 801
Bme18I GGWCC 3 cut(s) 178, 400, 565
BmeT110I CYCGRG 1 cut(s) 240
BmgT120I GGNCC 5 cut(s) 115, 178, 226, 400, 565
BmiI GGNNCC 3 cut(s) 179, 402, 567
BmrFI CCNGG 2 cut(s) 196, 801
BmsI GCATC 4 cut(s) 478, 951, 974, 1048
BmtI GCTAGC 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 675
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 899
BpuMI CCSGG 1 cut(s) 196
Bsa29I ATCGAT 1 cut(s) 216
BsaHI GRCGYC 1 cut(s) 543
BsaJI CCNNGG 5 cut(s) 133, 229, 363, 620, 799
BsaXI ACNNNNNCTCC 4 cut(s) 311, 341, 360, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 369
Bse1I ACTGG 1 cut(s) 480
Bse3DI GCAATG 3 cut(s) 298, 907, 1056
BseBI CCWGG 1 cut(s) 801
BseCI ATCGAT 1 cut(s) 216
BseDI CCNNGG 5 cut(s) 133, 229, 363, 620, 799
BseGI GGATG 2 cut(s) 469, 968
BseLI CCNNNNNNNGG 1 cut(s) 369
BseMI GCAATG 3 cut(s) 298, 907, 1056
BseNI ACTGG 1 cut(s) 480
BseRI GAGGAG 2 cut(s) 81, 518
BseXI GCAGC 4 cut(s) 85, 165, 783, 928
BsgI GTGCAG 3 cut(s) 92, 172, 935
Bsh1236I CGCG 1 cut(s) 579
BshFI GGCC 2 cut(s) 116, 228
BshVI ATCGAT 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 240
BsiSI CCGG 2 cut(s) 181, 195
BslFI GGGAC 2 cut(s) 347, 1038
BslI CCNNNNNNNGG 1 cut(s) 369
BsmAI GTCTC 1 cut(s) 756
BsmFI GGGAC 2 cut(s) 347, 1038
BsmI GAATGC 1 cut(s) 176
BsnI GGCC 2 cut(s) 116, 228
BsoBI CYCGRG 1 cut(s) 240
Bsp119I TTCGAA 1 cut(s) 270
Bsp19I CCATGG 2 cut(s) 133, 363
BspACI CCGC 6 cut(s) 156, 174, 203, 317, 467, 991
BspANI GGCC 2 cut(s) 116, 228
BspDI ATCGAT 1 cut(s) 216
BspFNI CGCG 1 cut(s) 579
BspHI TCATGA 1 cut(s) 162
BspLI GGNNCC 3 cut(s) 179, 402, 567
BspOI GCTAGC 1 cut(s) 188
BspT104I TTCGAA 1 cut(s) 270
BsrDI GCAATG 3 cut(s) 298, 907, 1056
BsrI ACTGG 1 cut(s) 480
BssECI CCNNGG 5 cut(s) 133, 229, 363, 620, 799
BssNI GRCGYC 1 cut(s) 543
BssT1I CCWWGG 2 cut(s) 133, 363
Bst2UI CCWGG 1 cut(s) 801
Bst4CI ACNGT 3 cut(s) 674, 727, 871
Bst6I CTCTTC 2 cut(s) 109, 318
BstACI GRCGYC 1 cut(s) 543
BstAPI GCANNNNNTGC 1 cut(s) 150
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 2 cut(s) 186, 769
BstDEI CTNAG 2 cut(s) 647, 1045
BstDSI CCRYGG 3 cut(s) 133, 229, 363
BstF5I GGATG 2 cut(s) 469, 968
BstFNI CGCG 1 cut(s) 579
BstHHI GCGC 1 cut(s) 579
BstMAI GTCTC 1 cut(s) 756
BstMWI GCNNNNNNNGC 3 cut(s) 150, 200, 883
BstNI CCWGG 1 cut(s) 801
BstSCI CCNGG 2 cut(s) 194, 799
BstSFI CTRYAG 1 cut(s) 891
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 4 cut(s) 85, 165, 783, 928
BstV2I GAAGAC 1 cut(s) 675
Bsu15I ATCGAT 1 cut(s) 216
BsuRI GGCC 2 cut(s) 116, 228
BsuTUI ATCGAT 1 cut(s) 216
BtgI CCRYGG 3 cut(s) 133, 229, 363
BtsCI GGATG 2 cut(s) 469, 968
BtsI GCAGTG 1 cut(s) 381
BtsIMutI CAGTG 2 cut(s) 381, 473
Cac8I GCNNGC 2 cut(s) 186, 769
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 1 cut(s) 162
CfoI GCGC 1 cut(s) 579
Cfr13I GGNCC 5 cut(s) 115, 178, 226, 400, 565
ClaI ATCGAT 1 cut(s) 216
Csp6I GTAC 4 cut(s) 328, 530, 757, 796
CviAII CATG 9 cut(s) 134, 145, 163, 364, 658, 680, 812, 907, 982
CviQI GTAC 4 cut(s) 328, 530, 757, 796
DdeI CTNAG 2 cut(s) 647, 1045
DrdI GACNNNNNNGTC 1 cut(s) 902
DseDI GACNNNNNNGTC 1 cut(s) 902
Eam1104I CTCTTC 2 cut(s) 109, 318
EarI CTCTTC 2 cut(s) 109, 318
Eco130I CCWWGG 2 cut(s) 133, 363
Eco32I GATATC 1 cut(s) 450
Eco47I GGWCC 3 cut(s) 178, 400, 565
Eco57I CTGAAG 1 cut(s) 759
Eco88I CYCGRG 1 cut(s) 240
EcoO109I RGGNCCY 3 cut(s) 115, 400, 565
EcoRI GAATTC 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 799
EcoRV GATATC 1 cut(s) 450
EcoT14I CCWWGG 2 cut(s) 133, 363
ErhI CCWWGG 2 cut(s) 133, 363
FaeI CATG 9 cut(s) 137, 148, 166, 367, 661, 683, 815, 910, 985
FaqI GGGAC 2 cut(s) 347, 1038
FatI CATG 9 cut(s) 133, 144, 162, 363, 657, 679, 811, 906, 981
FauI CCCGC 1 cut(s) 167
Fnu4HI GCNGC 6 cut(s) 74, 154, 467, 772, 917, 992
FokI GGATG 2 cut(s) 456, 955
Fsp4HI GCNGC 6 cut(s) 74, 154, 467, 772, 917, 992
FspBI CTAG 3 cut(s) 185, 495, 654
GlaI GCGC 1 cut(s) 578
GluI GCNGC 6 cut(s) 74, 154, 467, 772, 917, 992
HaeIII GGCC 2 cut(s) 116, 228
HapII CCGG 2 cut(s) 181, 195
HhaI GCGC 1 cut(s) 579
Hin1I GRCGYC 1 cut(s) 543
Hin1II CATG 9 cut(s) 137, 148, 166, 367, 661, 683, 815, 910, 985
Hin6I GCGC 1 cut(s) 577
HinP1I GCGC 1 cut(s) 577
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 3 cut(s) 213, 359, 551
HpaI GTTAAC 1 cut(s) 283
HpaII CCGG 2 cut(s) 181, 195
HphI GGTGA 2 cut(s) 16, 697
Hpy166II GTNNAC 2 cut(s) 283, 677
Hpy188I TCNGA 2 cut(s) 212, 454
Hpy188III TCNNGA 5 cut(s) 13, 63, 163, 825, 946
Hpy8I GTNNAC 2 cut(s) 283, 677
Hpy99I CGWCG 1 cut(s) 848
HpyAV CCTTC 3 cut(s) 37, 391, 568
HpyCH4III ACNGT 3 cut(s) 674, 727, 871
HpyCH4IV ACGT 3 cut(s) 235, 543, 843
HpyCH4V TGCA 7 cut(s) 73, 87, 153, 291, 434, 860, 916
HpyF10VI GCNNNNNNNGC 3 cut(s) 150, 200, 883
HpyF3I CTNAG 2 cut(s) 647, 1045
HpySE526I ACGT 3 cut(s) 235, 543, 843
Hsp92I GRCGYC 1 cut(s) 543
Hsp92II CATG 9 cut(s) 137, 148, 166, 367, 661, 683, 815, 910, 985
HspAI GCGC 1 cut(s) 577
KspAI GTTAAC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 1002
Lsp1109I GCAGC 4 cut(s) 85, 165, 783, 928
LweI GCATC 4 cut(s) 478, 951, 974, 1048
MaeI CTAG 3 cut(s) 185, 495, 654
MaeII ACGT 3 cut(s) 235, 543, 843
MaeIII GTNAC 3 cut(s) 137, 254, 727
MboII GAAGA 6 cut(s) 96, 292, 335, 535, 680, 1041
MluCI AATT 5 cut(s) 35, 58, 407, 791, 921
MnlI CCTC 8 cut(s) 59, 217, 313, 362, 496, 615, 676, 709
MseI TTAA 5 cut(s) 261, 282, 818, 933, 1019
MslI CAYNNNNRTG 2 cut(s) 439, 911
MspA1I CMGCKG 1 cut(s) 156
MspI CCGG 2 cut(s) 181, 195
MspR9I CCNGG 2 cut(s) 196, 801
Mva1269I GAATGC 1 cut(s) 176
MvaI CCWGG 1 cut(s) 801
MvnI CGCG 1 cut(s) 579
MwoI GCNNNNNNNGC 3 cut(s) 150, 200, 883
NciI CCSGG 1 cut(s) 196
NcoI CCATGG 2 cut(s) 133, 363
NheI GCTAGC 1 cut(s) 184
NlaIII CATG 9 cut(s) 137, 148, 166, 367, 661, 683, 815, 910, 985
NlaIV GGNNCC 3 cut(s) 179, 402, 567
NmeAIII GCCGAG 1 cut(s) 645
NspV TTCGAA 1 cut(s) 270
OliI CACNNNNGTG 1 cut(s) 439
PagI TCATGA 1 cut(s) 162
PcsI WCGNNNNNNNCGW 2 cut(s) 276, 849
PctI GAATGC 1 cut(s) 176
PfeI GAWTC 3 cut(s) 213, 359, 551
PkrI GCNGC 6 cut(s) 75, 155, 468, 773, 918, 993
PpuMI RGGWCCY 2 cut(s) 400, 565
Psp5II RGGWCCY 2 cut(s) 400, 565
Psp6I CCWGG 1 cut(s) 799
PspGI CCWGG 1 cut(s) 799
PspN4I GGNNCC 3 cut(s) 179, 402, 567
PspPI GGNCC 5 cut(s) 115, 178, 226, 400, 565
PspPPI RGGWCCY 2 cut(s) 400, 565
PsrI GAACNNNNNNTAC 2 cut(s) 191, 223
PstNI CAGNNNCTG 1 cut(s) 737
RsaI GTAC 4 cut(s) 329, 531, 758, 797
RsaNI GTAC 4 cut(s) 328, 530, 757, 796
RseI CAYNNNNRTG 2 cut(s) 439, 911
SaqAI TTAA 5 cut(s) 261, 282, 818, 933, 1019
SatI GCNGC 6 cut(s) 74, 154, 467, 772, 917, 992
Sau96I GGNCC 5 cut(s) 115, 178, 226, 400, 565
ScrFI CCNGG 2 cut(s) 196, 801
SfaNI GCATC 4 cut(s) 478, 951, 974, 1048
SfcI CTRYAG 1 cut(s) 891
SfuI TTCGAA 1 cut(s) 270
SinI GGWCC 3 cut(s) 178, 400, 565
SmiMI CAYNNNNRTG 2 cut(s) 439, 911
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
Sse9I AATT 5 cut(s) 35, 58, 407, 791, 921
SsiI CCGC 6 cut(s) 156, 174, 203, 317, 467, 991
SspMI CTAG 3 cut(s) 185, 495, 654
StyD4I CCNGG 2 cut(s) 194, 799
StyI CCWWGG 2 cut(s) 133, 363
TaaI ACNGT 3 cut(s) 674, 727, 871
TaiI ACGT 3 cut(s) 238, 546, 846
TaqI TCGA 4 cut(s) 216, 270, 308, 846
TasI AATT 5 cut(s) 35, 58, 407, 791, 921
TatI WGTACW 1 cut(s) 327
TauI GCSGC 2 cut(s) 469, 994
TfiI GAWTC 3 cut(s) 213, 359, 551
Tru1I TTAA 5 cut(s) 261, 282, 818, 933, 1019
Tru9I TTAA 5 cut(s) 261, 282, 818, 933, 1019
TscAI CASTG 2 cut(s) 381, 480
TseI GCWGC 4 cut(s) 73, 153, 771, 916
TspDTI ATGAA 4 cut(s) 151, 179, 380, 998
TspGWI ACGGA 1 cut(s) 246
TspRI CASTG 2 cut(s) 381, 480
VpaK11BI GGWCC 3 cut(s) 178, 400, 565
XapI RAATTY 2 cut(s) 58, 407
XspI CTAG 3 cut(s) 185, 495, 654
ZraI GACGTC 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.