FvH4_2g35160

Oleosin 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0007663
Physical Location & Seq
Reverse (-)
25977075 .. 25977730
656 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35160.t1

Sequence Viewer

Length: 405 bp
ATGGCCACAGCAGACTTTCATCACAAGCACGACGTCGTCGACGTCGATCAGTACCACTCATCCCCATCCTCACAAGGCAGGACTAGCATAATAACCAGGTTCTTGACTGCCGGCGCTATTGGCACCACGCTGGTAGGGCTGTCTGGGTTAACTTTAACCGGGACCGTGATTGCTCTTATCATGGCCACCCCGGTTCTAGTGTTTTTCAGTCCGGTCCTAGTCCCGGCGGCGATAGTCATGTTCCTAATAGCAGCTGGTTTTGTTTTCTCTGGCGGGTGTGGCGTGGCGGCGATCACGGCGCTGACGTGGTTGTACAAGTATGTATCCGACTACATCTCTACCGGGAGAAGGAATTACACTTATGACGGGTCTTACGCTAGCCGGGCTCAGTTTGGTCGACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oleosin

Protein Analysis

135

Amino Acids

14.27

Weight (kDa)

8.85

Isoelectric Point (pI)

33.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oleosin PF01277 31 - 117 8.5e-31 Oleosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 36, 45
AccB1I GGYRCC 1 cut(s) 122
AccI GTMKAC 2 cut(s) 39, 397
AciI CCGC 3 cut(s) 227, 273, 287
AcoI YGGCCR 2 cut(s) 3, 183
AcyI GRCGYC 2 cut(s) 33, 42
AfaI GTAC 2 cut(s) 53, 314
AfiI CCNNNNNNNGG 2 cut(s) 223, 348
AjiI CACGTC 1 cut(s) 306
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 1 cut(s) 254
AluI AGCT 1 cut(s) 254
AoxI GGCC 2 cut(s) 3, 183
ApeKI GCWGC 1 cut(s) 251
AspLEI GCGC 2 cut(s) 116, 301
AspS9I GGNCC 2 cut(s) 162, 214
AsuC2I CCSGG 5 cut(s) 160, 191, 224, 343, 383
AsuNHI GCTAGC 1 cut(s) 377
AvaII GGWCC 2 cut(s) 162, 214
BalI TGGCCA 2 cut(s) 5, 185
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 263
BccI CCATC 1 cut(s) 73
BceAI ACGGC 1 cut(s) 312
BciT130I CCWGG 1 cut(s) 97
BciVI GTATCC 1 cut(s) 334
BcnI CCSGG 5 cut(s) 160, 191, 224, 343, 383
BfaI CTAG 4 cut(s) 84, 197, 218, 378
BfoI RGCGCY 2 cut(s) 117, 302
BfuI GTATCC 1 cut(s) 334
BisI GCNGC 3 cut(s) 228, 252, 288
BlsI GCNGC 3 cut(s) 229, 253, 289
Bme1390I CCNGG 6 cut(s) 97, 160, 191, 224, 343, 383
Bme18I GGWCC 2 cut(s) 162, 214
BmgBI CACGTC 1 cut(s) 306
BmgT120I GGNCC 2 cut(s) 162, 214
BmiI GGNNCC 2 cut(s) 124, 163
BmrFI CCNGG 6 cut(s) 97, 160, 191, 224, 343, 383
BmtI GCTAGC 1 cut(s) 381
BpuMI CCSGG 5 cut(s) 160, 191, 224, 343, 383
BsaHI GRCGYC 2 cut(s) 33, 42
BsaJI CCNNGG 1 cut(s) 189
BsaWI WCCGGW 1 cut(s) 211
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 348
Bse118I RCCGGY 1 cut(s) 110
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 189
BseGI GGATG 2 cut(s) 59, 65
BseLI CCNNNNNNNGG 2 cut(s) 223, 348
BseMII CTCAG 1 cut(s) 401
BseXI GCAGC 1 cut(s) 263
BshFI GGCC 2 cut(s) 5, 185
BshNI GGYRCC 1 cut(s) 122
BsiSI CCGG 7 cut(s) 111, 159, 191, 212, 224, 342, 382
BslFI GGGAC 2 cut(s) 175, 206
BslI CCNNNNNNNGG 2 cut(s) 223, 348
BsmFI GGGAC 2 cut(s) 175, 206
BsnI GGCC 2 cut(s) 5, 185
Bsp1286I GDGCHC 1 cut(s) 388
Bsp1407I TGTACA 1 cut(s) 312
Bsp143I GATC 2 cut(s) 46, 291
BspACI CCGC 3 cut(s) 227, 273, 287
BspANI GGCC 2 cut(s) 5, 185
BspCNI CTCAG 1 cut(s) 400
BspLI GGNNCC 2 cut(s) 124, 163
BspOI GCTAGC 1 cut(s) 381
BspT107I GGYRCC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 110
BsrGI TGTACA 1 cut(s) 312
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 189
BssMI GATC 2 cut(s) 46, 291
BssNI GRCGYC 2 cut(s) 33, 42
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 166
BstACI GRCGYC 2 cut(s) 33, 42
BstAUI TGTACA 1 cut(s) 312
BstC8I GCNNGC 2 cut(s) 112, 379
BstDEI CTNAG 1 cut(s) 387
BstF5I GGATG 2 cut(s) 59, 65
BstH2I RGCGCY 2 cut(s) 117, 302
BstHHI GCGC 2 cut(s) 116, 301
BstKTI GATC 2 cut(s) 49, 294
BstMBI GATC 2 cut(s) 46, 291
BstMWI GCNNNNNNNGC 6 cut(s) 84, 120, 136, 279, 296, 383
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 6 cut(s) 95, 158, 189, 222, 341, 381
BstV1I GCAGC 1 cut(s) 263
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 2 cut(s) 5, 185
BtrI CACGTC 1 cut(s) 306
BtsCI GGATG 2 cut(s) 59, 65
Cac8I GCNNGC 2 cut(s) 112, 379
CfoI GCGC 2 cut(s) 116, 301
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 162, 214
CsiI ACCWGGT 1 cut(s) 95
Csp6I GTAC 2 cut(s) 52, 313
CviAII CATG 2 cut(s) 181, 238
CviJI RGCY 6 cut(s) 5, 139, 185, 254, 381, 386
CviKI_1 RGCY 6 cut(s) 5, 139, 185, 254, 381, 386
CviQI GTAC 2 cut(s) 52, 313
DdeI CTNAG 1 cut(s) 387
DpnI GATC 2 cut(s) 48, 293
DpnII GATC 2 cut(s) 46, 291
EaeI YGGCCR 2 cut(s) 3, 183
Eco24I GRGCYC 1 cut(s) 388
Eco47I GGWCC 2 cut(s) 162, 214
EcoRII CCWGG 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 388
FaeI CATG 2 cut(s) 184, 241
FaiI YATR 6 cut(s) 89, 182, 239, 321, 363, 403
FaqI GGGAC 2 cut(s) 175, 206
FatI CATG 2 cut(s) 180, 237
FauI CCCGC 1 cut(s) 266
FblI GTMKAC 2 cut(s) 39, 397
Fnu4HI GCNGC 3 cut(s) 228, 252, 288
FokI GGATG 2 cut(s) 46, 52
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 3 cut(s) 228, 252, 288
FspBI CTAG 4 cut(s) 84, 197, 218, 378
GlaI GCGC 2 cut(s) 115, 300
GluI GCNGC 3 cut(s) 228, 252, 288
HaeII RGCGCY 2 cut(s) 117, 302
HaeIII GGCC 2 cut(s) 5, 185
HapII CCGG 7 cut(s) 111, 159, 191, 212, 224, 342, 382
HhaI GCGC 2 cut(s) 116, 301
Hin1I GRCGYC 2 cut(s) 33, 42
Hin1II CATG 2 cut(s) 184, 241
Hin6I GCGC 2 cut(s) 114, 299
HinP1I GCGC 2 cut(s) 114, 299
HincII GTYRAC 3 cut(s) 40, 150, 398
HindII GTYRAC 3 cut(s) 40, 150, 398
HpaI GTTAAC 1 cut(s) 150
HpaII CCGG 7 cut(s) 111, 159, 191, 212, 224, 342, 382
Hpy166II GTNNAC 3 cut(s) 40, 150, 398
Hpy188I TCNGA 1 cut(s) 328
Hpy188III TCNNGA 1 cut(s) 103
Hpy8I GTNNAC 3 cut(s) 40, 150, 398
Hpy99I CGWCG 5 cut(s) 35, 38, 41, 44, 47
HpyAV CCTTC 1 cut(s) 342
HpyCH4III ACNGT 1 cut(s) 166
HpyCH4IV ACGT 3 cut(s) 33, 42, 305
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 120, 136, 279, 296, 383
HpyF3I CTNAG 1 cut(s) 387
HpySE526I ACGT 3 cut(s) 33, 42, 305
Hsp92I GRCGYC 2 cut(s) 33, 42
Hsp92II CATG 2 cut(s) 184, 241
HspAI GCGC 2 cut(s) 114, 299
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
KspAI GTTAAC 1 cut(s) 150
Kzo9I GATC 2 cut(s) 46, 291
Lsp1109I GCAGC 1 cut(s) 263
MabI ACCWGGT 1 cut(s) 95
MaeI CTAG 4 cut(s) 84, 197, 218, 378
MaeII ACGT 3 cut(s) 33, 42, 305
MalI GATC 2 cut(s) 48, 293
MboI GATC 2 cut(s) 46, 291
MhlI GDGCHC 1 cut(s) 388
MlsI TGGCCA 2 cut(s) 5, 185
MluCI AATT 1 cut(s) 352
MluNI TGGCCA 2 cut(s) 5, 185
MmeI TCCRAC 1 cut(s) 351
MnlI CCTC 1 cut(s) 79
Mox20I TGGCCA 2 cut(s) 5, 185
MroNI GCCGGC 1 cut(s) 110
MscI TGGCCA 2 cut(s) 5, 185
MseI TTAA 2 cut(s) 149, 155
Msp20I TGGCCA 2 cut(s) 5, 185
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 7 cut(s) 111, 159, 191, 212, 224, 342, 382
MspR9I CCNGG 6 cut(s) 97, 160, 191, 224, 343, 383
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 6 cut(s) 84, 120, 136, 279, 296, 383
NaeI GCCGGC 1 cut(s) 112
NciI CCSGG 5 cut(s) 160, 191, 224, 343, 383
NdeII GATC 2 cut(s) 46, 291
NgoMIV GCCGGC 1 cut(s) 110
NheI GCTAGC 1 cut(s) 377
NlaIII CATG 2 cut(s) 184, 241
NlaIV GGNNCC 2 cut(s) 124, 163
PcsI WCGNNNNNNNCGW 4 cut(s) 36, 39, 42, 302
PdiI GCCGGC 1 cut(s) 112
PflFI GACNNNGTC 1 cut(s) 35
PkrI GCNGC 3 cut(s) 229, 253, 289
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 2 cut(s) 124, 163
PspPI GGNCC 2 cut(s) 162, 214
PsyI GACNNNGTC 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 2 cut(s) 53, 314
RsaNI GTAC 2 cut(s) 52, 313
SalI GTCGAC 2 cut(s) 38, 396
SaqAI TTAA 2 cut(s) 149, 155
SatI GCNGC 3 cut(s) 228, 252, 288
Sau3AI GATC 2 cut(s) 46, 291
Sau96I GGNCC 2 cut(s) 162, 214
ScrFI CCNGG 6 cut(s) 97, 160, 191, 224, 343, 383
SduI GDGCHC 1 cut(s) 388
SetI ASST 5 cut(s) 36, 45, 101, 256, 308
SexAI ACCWGGT 1 cut(s) 95
SgrDI CGTCGACG 1 cut(s) 38
SinI GGWCC 2 cut(s) 162, 214
Sse9I AATT 1 cut(s) 352
SsiI CCGC 3 cut(s) 227, 273, 287
SspMI CTAG 4 cut(s) 84, 197, 218, 378
StyD4I CCNGG 6 cut(s) 95, 158, 189, 222, 341, 381
TaaI ACNGT 1 cut(s) 166
TaiI ACGT 3 cut(s) 36, 45, 308
TaqI TCGA 3 cut(s) 39, 45, 397
TasI AATT 1 cut(s) 352
TatI WGTACW 1 cut(s) 312
TauI GCSGC 2 cut(s) 230, 290
Tru1I TTAA 2 cut(s) 149, 155
Tru9I TTAA 2 cut(s) 149, 155
TseI GCWGC 1 cut(s) 251
TspDTI ATGAA 1 cut(s) 8
Tth111I GACNNNGTC 1 cut(s) 35
VpaK11BI GGWCC 2 cut(s) 162, 214
XmiI GTMKAC 2 cut(s) 39, 397
XspI CTAG 4 cut(s) 84, 197, 218, 378
ZraI GACGTC 2 cut(s) 34, 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.