RchiOBHm_Chr6g0306791

Oleosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007663
Physical Location & Seq
Forward (+)
65344705 .. 65345399
695 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27581

Sequence Viewer

Length: 378 bp
ATGATCAACATCAGGCCATTCGCCTCTTCCCAGAAGCCCAATGAGTCAGTGGCCTCACGCGACCGGACCAGCACTGCCAAGGTCTTGACCACTGGAGCAATCGGTGCCACTTTCTTGGTCTTGGCCGGGTTAACTTTAACCGGGACAGTGATGACCCTGATCTTGGCCAGCCCAGTTATAGTGCTGTTTAGTCCGGTCCTAGTCCCGGCTGGGATAGTCCTCTTCCTTGCAGCAAGTGGTTTGGATTTCTCCGGCGGGTGTGGCATGGTGGCGATTATGGCACTATCATGGTTGATTAAATACGCATCTGACTACCTCGCTGCCAAGAAGAGGGCTGACGCATATGGGGGCTCTAATTTCATGCAAATTGGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oleosin

Protein Analysis

125

Amino Acids

12.94

Weight (kDa)

9.58

Isoelectric Point (pI)

34.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oleosin PF01277 26 - 104 2.4e-26 Oleosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 104
AccII CGCG 1 cut(s) 60
AciI CCGC 1 cut(s) 255
AcoI YGGCCR 2 cut(s) 123, 165
AfiI CCNNNNNNNGG 3 cut(s) 163, 205, 330
AoxI GGCC 4 cut(s) 14, 51, 123, 165
ApeKI GCWGC 2 cut(s) 230, 320
AspS9I GGNCC 2 cut(s) 66, 196
AsuC2I CCSGG 3 cut(s) 127, 142, 206
AvaII GGWCC 2 cut(s) 66, 196
BalI TGGCCA 1 cut(s) 167
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 1 cut(s) 353
BbvI GCAGC 2 cut(s) 242, 307
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 3 cut(s) 127, 142, 206
BfaI CTAG 1 cut(s) 200
BisI GCNGC 2 cut(s) 231, 321
BlsI GCNGC 2 cut(s) 232, 322
Bme1390I CCNGG 3 cut(s) 127, 142, 206
Bme18I GGWCC 2 cut(s) 66, 196
BmgT120I GGNCC 2 cut(s) 66, 196
BmiI GGNNCC 1 cut(s) 106
BmrFI CCNGG 3 cut(s) 127, 142, 206
BmrI ACTGGG 1 cut(s) 167
BmsI GCATC 1 cut(s) 314
BmuI ACTGGG 1 cut(s) 167
BpmI CTGGAG 1 cut(s) 114
BpuMI CCSGG 3 cut(s) 127, 142, 206
BsaBI GATNNNNATC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 78
BsaWI WCCGGW 2 cut(s) 63, 193
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 205, 330
Bse1I ACTGG 2 cut(s) 97, 173
Bse8I GATNNNNATC 1 cut(s) 8
BseDI CCNNGG 1 cut(s) 78
BseJI GATNNNNATC 1 cut(s) 8
BseLI CCNNNNNNNGG 3 cut(s) 163, 205, 330
BseNI ACTGG 2 cut(s) 97, 173
BseXI GCAGC 2 cut(s) 242, 307
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 4 cut(s) 16, 53, 125, 167
BshNI GGYRCC 1 cut(s) 104
BsiEI CGRYCG 1 cut(s) 64
BsiSI CCGG 6 cut(s) 64, 126, 141, 194, 206, 252
BslFI GGGAC 2 cut(s) 157, 188
BslI CCNNNNNNNGG 3 cut(s) 163, 205, 330
BsmFI GGGAC 2 cut(s) 157, 188
BsnI GGCC 4 cut(s) 16, 53, 125, 167
Bsp1286I GDGCHC 1 cut(s) 353
Bsp143I GATC 2 cut(s) 3, 159
BspACI CCGC 1 cut(s) 255
BspANI GGCC 4 cut(s) 16, 53, 125, 167
BspFNI CGCG 1 cut(s) 60
BspLI GGNNCC 1 cut(s) 106
BspT107I GGYRCC 1 cut(s) 104
BsrI ACTGG 2 cut(s) 97, 173
BssECI CCNNGG 1 cut(s) 78
BssMI GATC 2 cut(s) 3, 159
BssT1I CCWWGG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 3 cut(s) 31, 227, 323
BstAPI GCANNNNNTGC 1 cut(s) 104
BstC8I GCNNGC 1 cut(s) 169
BstFNI CGCG 1 cut(s) 60
BstKTI GATC 2 cut(s) 6, 162
BstMBI GATC 2 cut(s) 3, 159
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 3 cut(s) 104, 261, 278
BstSCI CCNGG 3 cut(s) 125, 140, 204
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 2 cut(s) 242, 307
BstXI CCANNNNNNTGG 1 cut(s) 115
BsuRI GGCC 4 cut(s) 16, 53, 125, 167
BtsI GCAGTG 1 cut(s) 72
BtsIMutI CAGTG 4 cut(s) 54, 72, 90, 153
Cac8I GCNNGC 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 66, 196
CseI GACGC 1 cut(s) 347
CviAII CATG 3 cut(s) 265, 288, 361
CviJI RGCY 9 cut(s) 16, 37, 53, 125, 167, 171, 209, 335, 351
CviKI_1 RGCY 9 cut(s) 16, 37, 53, 125, 167, 171, 209, 335, 351
DpnI GATC 2 cut(s) 5, 161
DpnII GATC 2 cut(s) 3, 159
EaeI YGGCCR 2 cut(s) 123, 165
Eam1104I CTCTTC 3 cut(s) 31, 227, 323
EarI CTCTTC 3 cut(s) 31, 227, 323
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 353
Eco47I GGWCC 2 cut(s) 66, 196
EcoT14I CCWWGG 1 cut(s) 78
EcoT38I GRGCYC 1 cut(s) 353
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 3 cut(s) 268, 291, 364
FaiI YATR 8 cut(s) 179, 266, 278, 289, 343, 345, 362, 376
FaqI GGGAC 2 cut(s) 157, 188
FatI CATG 3 cut(s) 264, 287, 360
FauI CCCGC 1 cut(s) 248
FauNDI CATATG 1 cut(s) 343
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 231, 321
FriOI GRGCYC 1 cut(s) 353
Fsp4HI GCNGC 2 cut(s) 231, 321
FspBI CTAG 1 cut(s) 200
GluI GCNGC 2 cut(s) 231, 321
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 4 cut(s) 16, 53, 125, 167
HapII CCGG 6 cut(s) 64, 126, 141, 194, 206, 252
HgaI GACGC 1 cut(s) 347
Hin1II CATG 3 cut(s) 268, 291, 364
HincII GTYRAC 1 cut(s) 132
HindII GTYRAC 1 cut(s) 132
HinfI GANTC 1 cut(s) 44
HpaI GTTAAC 1 cut(s) 132
HpaII CCGG 6 cut(s) 64, 126, 141, 194, 206, 252
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 1 cut(s) 85
Hpy8I GTNNAC 1 cut(s) 132
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4V TGCA 2 cut(s) 230, 364
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 261, 278
Hsp92II CATG 3 cut(s) 268, 291, 364
Ksp22I TGATCA 1 cut(s) 3
KspAI GTTAAC 1 cut(s) 132
Kzo9I GATC 2 cut(s) 3, 159
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 2 cut(s) 242, 307
LweI GCATC 1 cut(s) 314
MaeI CTAG 1 cut(s) 200
MalI GATC 2 cut(s) 5, 161
MboI GATC 2 cut(s) 3, 159
MboII GAAGA 3 cut(s) 18, 214, 340
MhlI GDGCHC 1 cut(s) 353
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 2 cut(s) 355, 366
MluNI TGGCCA 1 cut(s) 167
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 5 cut(s) 34, 64, 230, 324, 326
Mox20I TGGCCA 1 cut(s) 167
MscI TGGCCA 1 cut(s) 167
MseI TTAA 3 cut(s) 131, 137, 297
MslI CAYNNNNRTG 1 cut(s) 286
Msp20I TGGCCA 1 cut(s) 167
MspI CCGG 6 cut(s) 64, 126, 141, 194, 206, 252
MspR9I CCNGG 3 cut(s) 127, 142, 206
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 3 cut(s) 104, 261, 278
NciI CCSGG 3 cut(s) 127, 142, 206
NdeI CATATG 1 cut(s) 343
NdeII GATC 2 cut(s) 3, 159
NlaIII CATG 3 cut(s) 268, 291, 364
NlaIV GGNNCC 1 cut(s) 106
PkrI GCNGC 2 cut(s) 232, 322
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PspFI CCCAGC 1 cut(s) 209
PspN4I GGNNCC 1 cut(s) 106
PspPI GGNCC 2 cut(s) 66, 196
RseI CAYNNNNRTG 1 cut(s) 286
SaqAI TTAA 3 cut(s) 131, 137, 297
SatI GCNGC 2 cut(s) 231, 321
Sau3AI GATC 2 cut(s) 3, 159
Sau96I GGNCC 2 cut(s) 66, 196
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 3 cut(s) 127, 142, 206
SduI GDGCHC 1 cut(s) 353
SetI ASST 2 cut(s) 84, 318
SfaNI GCATC 1 cut(s) 314
SinI GGWCC 2 cut(s) 66, 196
SmiMI CAYNNNNRTG 1 cut(s) 286
Sse9I AATT 2 cut(s) 355, 366
SsiI CCGC 1 cut(s) 255
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 3 cut(s) 125, 140, 204
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 148
TasI AATT 2 cut(s) 355, 366
Tru1I TTAA 3 cut(s) 131, 137, 297
Tru9I TTAA 3 cut(s) 131, 137, 297
TscAI CASTG 4 cut(s) 54, 79, 97, 153
TseI GCWGC 2 cut(s) 230, 320
TspDTI ATGAA 1 cut(s) 349
TspRI CASTG 4 cut(s) 54, 79, 97, 153
VpaK11BI GGWCC 2 cut(s) 66, 196
XcmI CCANNNNNNNNNTGG 1 cut(s) 46
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.