Rorug06G0352900

Oleosin 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007663
Physical Location & Seq
Forward (+)
50169114 .. 50170510
1397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0352900.1

Sequence Viewer

Length: 597 bp
ATGGCATACGCCAAAAAAAATTTGACATTCATTTTCTCTGCATTTCTGATCATTCCAACTCTTTTGGCAGCTGTAAATGGGGATCATAGCTATGTCGAAGACGCCTGCAGCGTGACGAGGTACCGTGACCTCTGCATCCACTCGCTAGCATCGTTTTCGAGCACAGCAAAGAATAGCCCCAGCAGGTGGGCTAGAGCCGGGGTTTCGGTGACATTGGGGGAGACCAAAAACGTTGCTCGGTACTTGGTCTATCTGAAGAAGCACAGCAACCGCATGAAGGGAAAAGCTGGAGTTGCTCTATCCGATTGCATTGAGGTTTTTGAGAATGCAATCGACGAGCTTCACAAATCACTTGGTGTGCTAAGAAAGCTGAGCAGAACTACATTCGCCACGCAAATGGGGGACCTTAATACATGGCTGAGCGCCGCCCTTACTGACGGAGACACTTGCTTGGACGGTTTTGAGGAGGTCCAAAGAGGAAAACAAATCAAATCGCTTCAAAATCGGGTTTTGAGAGCAACTCGTTTCACCAGTAACGCACTGGCTCTTGCTAACAAACTTGCTGCCACTGGCTTGAGAAGCCTCCCAGATCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Oleosin

Protein Analysis

198

Amino Acids

21.69

Weight (kDa)

9.56

Isoelectric Point (pI)

31.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 31 - 183 2.4e-37 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 174
Acc36I ACCTGC 1 cut(s) 174
Acc65I GGTACC 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 120
AccB7I CCANNNNNTGG 1 cut(s) 186
AciI CCGC 2 cut(s) 271, 426
AclI AACGTT 1 cut(s) 231
AclWI GGATC 2 cut(s) 90, 584
AcsI RAATTY 1 cut(s) 19
AcuI CTGAAG 1 cut(s) 275
AcyI GRCGYC 1 cut(s) 102
AdeI CACNNNGTG 1 cut(s) 356
AfaI GTAC 2 cut(s) 122, 242
AfiI CCNNNNNNNGG 2 cut(s) 186, 277
AgsI TTSAA 1 cut(s) 500
AluBI AGCT 5 cut(s) 71, 90, 287, 340, 370
AluI AGCT 5 cut(s) 71, 90, 287, 340, 370
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 2 cut(s) 215, 435
AlwI GGATC 2 cut(s) 90, 584
ApeKI GCWGC 3 cut(s) 68, 108, 563
ApoI RAATTY 1 cut(s) 19
Asp718I GGTACC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 425
AspS9I GGNCC 2 cut(s) 403, 469
AsuC2I CCSGG 1 cut(s) 199
AsuHPI GGTGA 2 cut(s) 220, 520
AsuNHI GCTAGC 1 cut(s) 145
AvaII GGWCC 2 cut(s) 403, 469
BanI GGYRCC 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 3 cut(s) 80, 120, 550
BcgI CGANNNNNNTGC 2 cut(s) 138, 172
BclI TGATCA 1 cut(s) 48
BcnI CCSGG 1 cut(s) 199
BcoDI GTCTC 2 cut(s) 215, 435
BfaI CTAG 2 cut(s) 146, 192
BfmI CTRYAG 1 cut(s) 106
BfoI RGCGCY 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 174
BisI GCNGC 4 cut(s) 69, 109, 426, 564
BlpI GCTNAGC 2 cut(s) 371, 419
BlsI GCNGC 4 cut(s) 70, 110, 427, 565
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 2 cut(s) 403, 469
BmgT120I GGNCC 2 cut(s) 403, 469
BmiI GGNNCC 2 cut(s) 122, 404
BmrFI CCNGG 1 cut(s) 199
BmsI GCATC 2 cut(s) 144, 158
BmtI GCTAGC 1 cut(s) 149
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 505, 537
BpmI CTGGAG 1 cut(s) 309
Bpu1102I GCTNAGC 2 cut(s) 371, 419
BpuEI CTTGAG 1 cut(s) 595
BpuMI CCSGG 1 cut(s) 199
BsaHI GRCGYC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 1 cut(s) 198
Bsc4I CCNNNNNNNGG 2 cut(s) 186, 277
Bse1I ACTGG 3 cut(s) 531, 546, 574
BseDI CCNNGG 1 cut(s) 198
BseGI GGATG 1 cut(s) 135
BseLI CCNNNNNNNGG 2 cut(s) 186, 277
BseMII CTCAG 2 cut(s) 362, 410
BseNI ACTGG 3 cut(s) 531, 546, 574
BseRI GAGGAG 1 cut(s) 479
BseXI GCAGC 3 cut(s) 80, 120, 550
BseYI CCCAGC 1 cut(s) 179
BshNI GGYRCC 1 cut(s) 120
BsiHKAI GWGCWC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 198
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 2 cut(s) 186, 277
BsmAI GTCTC 2 cut(s) 215, 435
BsmFI GGGAC 1 cut(s) 416
BsmI GAATGC 1 cut(s) 331
Bso31I GGTCTC 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 164
Bsp143I GATC 3 cut(s) 48, 82, 589
Bsp1720I GCTNAGC 2 cut(s) 371, 419
BspACI CCGC 2 cut(s) 271, 426
BspCNI CTCAG 2 cut(s) 363, 411
BspLI GGNNCC 2 cut(s) 122, 404
BspMAI CTGCAG 1 cut(s) 110
BspMI ACCTGC 1 cut(s) 174
BspOI GCTAGC 1 cut(s) 149
BspPI GGATC 2 cut(s) 90, 584
BspT107I GGYRCC 1 cut(s) 120
BspTNI GGTCTC 1 cut(s) 215
BsrI ACTGG 3 cut(s) 531, 546, 574
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 3 cut(s) 48, 82, 589
BssNI GRCGYC 1 cut(s) 102
Bst4CI ACNGT 2 cut(s) 125, 458
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 2 cut(s) 106, 147
BstDEI CTNAG 3 cut(s) 362, 371, 419
BstF5I GGATG 1 cut(s) 135
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 1 cut(s) 425
BstKTI GATC 3 cut(s) 51, 85, 592
BstMAI GTCTC 2 cut(s) 215, 435
BstMBI GATC 3 cut(s) 48, 82, 589
BstMWI GCNNNNNNNGC 3 cut(s) 293, 367, 579
BstSCI CCNGG 1 cut(s) 197
BstSFI CTRYAG 1 cut(s) 106
BstV1I GCAGC 3 cut(s) 80, 120, 550
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 589
BstXI CCANNNNNNTGG 1 cut(s) 397
BstYI RGATCY 1 cut(s) 589
BtsCI GGATG 1 cut(s) 135
BtsIMutI CAGTG 2 cut(s) 539, 567
BveI ACCTGC 1 cut(s) 174
Cac8I GCNNGC 2 cut(s) 106, 147
CfoI GCGC 1 cut(s) 425
Cfr13I GGNCC 2 cut(s) 403, 469
CseI GACGC 1 cut(s) 110
Csp6I GTAC 2 cut(s) 121, 241
CviAII CATG 2 cut(s) 274, 414
CviQI GTAC 2 cut(s) 121, 241
DdeI CTNAG 3 cut(s) 362, 371, 419
DpnI GATC 3 cut(s) 50, 84, 591
DpnII GATC 3 cut(s) 48, 82, 589
DraIII CACNNNGTG 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 215
Eco47I GGWCC 2 cut(s) 403, 469
Eco57I CTGAAG 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 403
FaeI CATG 2 cut(s) 277, 417
FaiI YATR 6 cut(s) 7, 87, 93, 275, 415, 595
FaqI GGGAC 1 cut(s) 416
FatI CATG 2 cut(s) 273, 413
FbaI TGATCA 1 cut(s) 48
Fnu4HI GCNGC 4 cut(s) 69, 109, 426, 564
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 4 cut(s) 69, 109, 426, 564
FspBI CTAG 2 cut(s) 146, 192
GlaI GCGC 1 cut(s) 424
GluI GCNGC 4 cut(s) 69, 109, 426, 564
GsaI CCCAGC 1 cut(s) 183
GsuI CTGGAG 1 cut(s) 309
HaeII RGCGCY 1 cut(s) 426
HapII CCGG 1 cut(s) 198
HgaI GACGC 1 cut(s) 110
HhaI GCGC 1 cut(s) 425
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 2 cut(s) 277, 417
Hin6I GCGC 1 cut(s) 423
HinP1I GCGC 1 cut(s) 423
HpaII CCGG 1 cut(s) 198
HphI GGTGA 2 cut(s) 220, 520
Hpy188I TCNGA 3 cut(s) 48, 255, 304
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 2 cut(s) 125, 458
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 5 cut(s) 41, 108, 135, 309, 329
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 367, 579
HpyF3I CTNAG 3 cut(s) 362, 371, 419
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 2 cut(s) 277, 417
HspAI GCGC 1 cut(s) 423
KpnI GGTACC 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 48
Kzo9I GATC 3 cut(s) 48, 82, 589
LpnPI CCDG 8 cut(s) 118, 169, 193, 211, 273, 527, 544, 555
Lsp1109I GCAGC 3 cut(s) 80, 120, 550
LweI GCATC 2 cut(s) 144, 158
MaeI CTAG 2 cut(s) 146, 192
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 4 cut(s) 112, 125, 208, 533
MalI GATC 3 cut(s) 50, 84, 591
MboI GATC 3 cut(s) 48, 82, 589
MboII GAAGA 2 cut(s) 110, 268
MflI RGATCY 1 cut(s) 589
MhlI GDGCHC 1 cut(s) 164
MluCI AATT 1 cut(s) 19
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 7 cut(s) 111, 140, 307, 457, 460, 470, 593
MseI TTAA 1 cut(s) 408
MslI CAYNNNNRTG 2 cut(s) 90, 395
MspA1I CMGCKG 1 cut(s) 71
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 1 cut(s) 199
Mva1269I GAATGC 1 cut(s) 331
MwoI GCNNNNNNNGC 3 cut(s) 293, 367, 579
NciI CCSGG 1 cut(s) 199
NdeII GATC 3 cut(s) 48, 82, 589
NheI GCTAGC 1 cut(s) 145
NlaIII CATG 2 cut(s) 277, 417
NlaIV GGNNCC 2 cut(s) 122, 404
NmuCI GTSAC 3 cut(s) 112, 125, 208
PaqCI CACCTGC 1 cut(s) 174
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 149
PctI GAATGC 1 cut(s) 331
PflMI CCANNNNNTGG 1 cut(s) 186
PkrI GCNGC 4 cut(s) 70, 110, 427, 565
PpuMI RGGWCCY 1 cut(s) 403
Psp1406I AACGTT 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 403
PspFI CCCAGC 1 cut(s) 179
PspN4I GGNNCC 2 cut(s) 122, 404
PspPI GGNCC 2 cut(s) 403, 469
PspPPI RGGWCCY 1 cut(s) 403
PstI CTGCAG 1 cut(s) 110
PsuI RGATCY 1 cut(s) 589
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 2 cut(s) 122, 242
RsaNI GTAC 2 cut(s) 121, 241
RseI CAYNNNNRTG 2 cut(s) 90, 395
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 4 cut(s) 69, 109, 426, 564
Sau3AI GATC 3 cut(s) 48, 82, 589
Sau96I GGNCC 2 cut(s) 403, 469
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 1 cut(s) 164
SfaNI GCATC 2 cut(s) 144, 158
SfcI CTRYAG 1 cut(s) 106
SinI GGWCC 2 cut(s) 403, 469
SmiMI CAYNNNNRTG 2 cut(s) 90, 395
SmlI CTYRAG 1 cut(s) 574
SmoI CTYRAG 1 cut(s) 574
Sse9I AATT 1 cut(s) 19
SsiI CCGC 2 cut(s) 271, 426
SspMI CTAG 2 cut(s) 146, 192
StyD4I CCNGG 1 cut(s) 197
TaaI ACNGT 2 cut(s) 125, 458
TaiI ACGT 1 cut(s) 234
TaqI TCGA 3 cut(s) 96, 158, 333
TasI AATT 1 cut(s) 19
TauI GCSGC 1 cut(s) 428
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 2 cut(s) 546, 574
TseFI GTSAC 3 cut(s) 112, 125, 208
TseI GCWGC 3 cut(s) 68, 108, 563
Tsp45I GTSAC 3 cut(s) 112, 125, 208
TspDTI ATGAA 2 cut(s) 19, 290
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 2 cut(s) 546, 574
Van91I CCANNNNNTGG 1 cut(s) 186
VpaK11BI GGWCC 2 cut(s) 403, 469
XapI RAATTY 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XspI CTAG 2 cut(s) 146, 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.