FvH4_2g38890

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009780
Physical Location & Seq
Reverse (-)
28050132 .. 28053173
3042 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38890.t3

Sequence Viewer

Length: 1398 bp
ATGGACTGCTCTACGGGACTTGTATCGGCTACGATACTAGTACTGATTCTTTCTGTGAAGCTTTGTGAAAGTGGGATAACTAGTAGTTACTTGAGGAACGATGATCTTGTCCATGACATGCCTTTAGACTCTGATGTGTTCCGAGCACCTCCTGGTTATAATGCTCCTCAACAGGTTCATATAACACAAGGGGATCATGAAGGGAAGGGAGTGATTGTGTCTTGGGTGACCCCAAATGAACCTGGTTCAAGTACTGTGGTTTACTGGGCAGAGAATAGCAGCAATCTGAAAAAGACTGCTTATGGAATTGTTCTTACTTACAACTATTTCAATTACACTTCTGGTTACATTCACCACTGTACCATTTCCAATTTGGAGTTTGGCACCAAATACTATTATGAAGTTGGGACTGGGAATACCACAAGACGATTTTGGTTTATAACACCTCCTAAAGCTGGTCCTGACGTTCCCTATACTTTTGGCCTCATAGGGGATATTGGTCAGACCCCTGATTCAAACCGAACGCTTACTCATTACGAGAAGAATCCAGCAAAAGGACAGACGGTGTTGTTCGTTGGGGACCTCTCTTATGCAGACGCTTATCCATTTCATGACAATAGGAGATGGGATACATGGGGGAGATTCCTAGAGAGAAATGTTGCCTATCAGCCTTGGATATGGACTGCGGGAAATCATGAAATTGATTATGCTCCTCAATTTGGTGAAGGTATACCATTCAAACCATATACAAGTCGCTATTTTGTTCCATACAAAGCTTCAGATAGTACATCTCCTCTGTGGTACTCCATCAAGAGAGCTTCAGCATATATCATAGTTTTGTCTTCTTATTCGGCATATGGAAAATATACTCCTCAGTATAAGTGGCTTGAAAAGGAACTGCCTCAAGTGAACAGAACTGAGACACCATGGCTTATTGTGCTTGTCCATTGTCCAATGTATAGCAGCTATGTGCATCACTATATGGAAGGTGAAAGCATGAGAGTTATGTATGAGGAATGGTTTGTGGATTATAAAGTAGACGTTGTCTTTTCTGGACATGTTCATGCCTATGAACGATCTGAAAGGATATCAAACATTGCATACAACATCACAAATGGACTCTGCACTCCCACAAGTGACCAATCTGCCCCAGTTTACATAACAATTGGAGATGGAGGAAATCTAGAAGGATTGGTCACAGAAATGACAGAACCGCAGCCAAGCTACTCGGCATTTCGTGAACCTAGCTTTGGCCATGGGATTCTTGAGATAAAAAACAGAAGCTATGCCTTTTTCAGTTGGCATAGGAATCAAGATGGTGAAGCAGTAGAAGCTGATTCTTTATGGTTACGCAACAGGCATTGGAAAACTACTATGGAGCACTCATCATCCCTATAG

Protein Analysis

466

Amino Acids

53.13

Weight (kDa)

5.78

Isoelectric Point (pI)

41.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 65 - 157 2e-20 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 168 - 366 1.8e-26 Calcineurin-like phosphoesterase
Metallophos_C PF14008 392 - 451 2.1e-17 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009780)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52940 AT2G27190
fragaria_vesca FvH4_2g38890 FvH4_2g38890 FvH4_2g38890 FvH4_2g38890 FvH4_2g38890 FvH4_2g38890
malus_domestica MD15G1020000.v1.1
prunus_persica Prupe.1G372000_v2.0.a1
pyrus_communis pycom15g01660
rosa_chinensis RchiOBHm_Chr6g0301991
rosa_laevigata RLG00000011210
rosa_multiflora Rmu_sc0006892.1_g000004
rosa_roxburghii Rroxscaffold_7G00166210
rosa_rugosa Rorug06G0310900
rosa_samantha Rh6AG423400 Rh6BG430100 Rh6CG436500 Rh6DG423500
rosa_wichuraiana Rw6G036680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 159, 440, 1032
AccB1I GGYRCC 1 cut(s) 383
AccI GTMKAC 2 cut(s) 730, 1038
AciI CCGC 2 cut(s) 686, 1214
AclWI GGATC 1 cut(s) 201
AcoI YGGCCR 1 cut(s) 1252
AcuI CTGAAG 2 cut(s) 762, 804
AfaI GTAC 5 cut(s) 42, 253, 361, 787, 803
AfiI CCNNNNNNNGG 5 cut(s) 455, 490, 554, 719, 1250
AflIII ACRYGT 1 cut(s) 1057
AgsI TTSAA 5 cut(s) 249, 331, 516, 739, 890
AhlI ACTAGT 2 cut(s) 37, 80
AjnI CCWGG 2 cut(s) 151, 241
AjuI GAANNNNNNNTTGG 2 cut(s) 1233, 1265
AluBI AGCT 9 cut(s) 61, 455, 776, 818, 966, 1224, 1248, 1284, 1334
AluI AGCT 9 cut(s) 61, 455, 776, 818, 966, 1224, 1248, 1284, 1334
Alw21I GWGCWC 2 cut(s) 148, 1383
Alw26I GTCTC 1 cut(s) 914
AlwI GGATC 1 cut(s) 201
AoxI GGCC 2 cut(s) 481, 1252
ApeKI GCWGC 3 cut(s) 279, 963, 1216
AspS9I GGNCC 2 cut(s) 458, 580
AsuHPI GGTGA 5 cut(s) 238, 344, 734, 1001, 1331
AvaII GGWCC 2 cut(s) 458, 580
BaeI ACNNNNGTAYC 2 cut(s) 343, 376
BalI TGGCCA 1 cut(s) 1254
BanI GGYRCC 1 cut(s) 383
BbsI GAAGAC 1 cut(s) 834
Bbv12I GWGCWC 2 cut(s) 148, 1383
BbvI GCAGC 3 cut(s) 291, 975, 1228
BccI CCATC 4 cut(s) 618, 815, 1166, 1310
BciT130I CCWGG 2 cut(s) 153, 243
BciVI GTATCC 1 cut(s) 622
BcoDI GTCTC 1 cut(s) 914
BcuI ACTAGT 2 cut(s) 37, 80
BfaI CTAG 5 cut(s) 38, 81, 647, 1184, 1245
BfmI CTRYAG 1 cut(s) 1394
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 3 cut(s) 280, 964, 1217
BlsI GCNGC 3 cut(s) 281, 965, 1218
BmcAI AGTACT 2 cut(s) 42, 253
Bme1390I CCNGG 2 cut(s) 153, 243
Bme18I GGWCC 2 cut(s) 458, 580
BmgT120I GGNCC 2 cut(s) 458, 580
BmiI GGNNCC 2 cut(s) 385, 581
BmrFI CCNGG 2 cut(s) 153, 243
BmrI ACTGGG 3 cut(s) 274, 420, 1145
BmsI GCATC 1 cut(s) 982
BmuI ACTGGG 3 cut(s) 274, 420, 1145
BpiI GAAGAC 1 cut(s) 834
BpuEI CTTGAG 3 cut(s) 112, 888, 1286
BsaJI CCNNGG 3 cut(s) 671, 926, 1255
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 5 cut(s) 455, 490, 554, 719, 1250
Bse1I ACTGG 3 cut(s) 269, 415, 1151
Bse3DI GCAATG 1 cut(s) 1095
BseBI CCWGG 2 cut(s) 153, 243
BseDI CCNNGG 3 cut(s) 671, 926, 1255
BseGI GGATG 1 cut(s) 1388
BseLI CCNNNNNNNGG 5 cut(s) 455, 490, 554, 719, 1250
BseMI GCAATG 1 cut(s) 1095
BseMII CTCAG 2 cut(s) 887, 909
BseNI ACTGG 3 cut(s) 269, 415, 1151
BseRI GAGGAG 4 cut(s) 156, 702, 783, 861
BseXI GCAGC 3 cut(s) 291, 975, 1228
BsgI GTGCAG 1 cut(s) 1108
BshFI GGCC 2 cut(s) 483, 1254
BshNI GGYRCC 1 cut(s) 383
BsiHKAI GWGCWC 2 cut(s) 148, 1383
BslFI GGGAC 3 cut(s) 30, 421, 593
BslI CCNNNNNNNGG 5 cut(s) 455, 490, 554, 719, 1250
BsmAI GTCTC 1 cut(s) 914
BsmFI GGGAC 3 cut(s) 30, 421, 593
BsnI GGCC 2 cut(s) 483, 1254
Bsp1286I GDGCHC 2 cut(s) 148, 1383
Bsp143I GATC 3 cut(s) 103, 193, 1076
Bsp19I CCATGG 2 cut(s) 926, 1255
BspACI CCGC 2 cut(s) 686, 1214
BspANI GGCC 2 cut(s) 483, 1254
BspCNI CTCAG 2 cut(s) 886, 910
BspHI TCATGA 3 cut(s) 196, 610, 694
BspLI GGNNCC 2 cut(s) 385, 581
BspPI GGATC 1 cut(s) 201
BspT107I GGYRCC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 1095
BsrI ACTGG 3 cut(s) 269, 415, 1151
BssECI CCNNGG 3 cut(s) 671, 926, 1255
BssMI GATC 3 cut(s) 103, 193, 1076
BssNAI GTATAC 1 cut(s) 731
BssT1I CCWWGG 3 cut(s) 671, 926, 1255
Bst1107I GTATAC 1 cut(s) 731
Bst2UI CCWGG 2 cut(s) 153, 243
Bst4CI ACNGT 3 cut(s) 256, 359, 565
BstDEI CTNAG 2 cut(s) 873, 918
BstDSI CCRYGG 2 cut(s) 926, 1255
BstEII GGTNACC 1 cut(s) 226
BstF5I GGATG 1 cut(s) 1388
BstKTI GATC 3 cut(s) 106, 196, 1079
BstMAI GTCTC 1 cut(s) 914
BstMBI GATC 3 cut(s) 103, 193, 1076
BstMWI GCNNNNNNNGC 2 cut(s) 937, 1331
BstNI CCWGG 2 cut(s) 153, 243
BstNSI RCATGY 2 cut(s) 121, 1061
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 151, 241
BstSFI CTRYAG 1 cut(s) 1394
BstV1I GCAGC 3 cut(s) 291, 975, 1228
BstV2I GAAGAC 1 cut(s) 834
BstZ17I GTATAC 1 cut(s) 731
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 2 cut(s) 483, 1254
BtgI CCRYGG 2 cut(s) 926, 1255
BtsCI GGATG 1 cut(s) 1388
BtsIMutI CAGTG 1 cut(s) 355
CciI TCATGA 3 cut(s) 196, 610, 694
Cfr13I GGNCC 2 cut(s) 458, 580
CseI GACGC 1 cut(s) 605
CsiI ACCWGGT 1 cut(s) 241
Csp6I GTAC 5 cut(s) 41, 252, 360, 786, 802
CspCI CAANNNNNGTGG 2 cut(s) 237, 272
CviQI GTAC 5 cut(s) 41, 252, 360, 786, 802
DdeI CTNAG 2 cut(s) 873, 918
DpnI GATC 3 cut(s) 105, 195, 1078
DpnII GATC 3 cut(s) 103, 193, 1076
EaeI YGGCCR 1 cut(s) 1252
Eco130I CCWWGG 3 cut(s) 671, 926, 1255
Eco32I GATATC 1 cut(s) 1089
Eco47I GGWCC 2 cut(s) 458, 580
Eco57I CTGAAG 2 cut(s) 762, 804
Eco91I GGTNACC 1 cut(s) 226
EcoO109I RGGNCCY 1 cut(s) 580
EcoO65I GGTNACC 1 cut(s) 226
EcoRII CCWGG 2 cut(s) 151, 241
EcoRV GATATC 1 cut(s) 1089
EcoT14I CCWWGG 3 cut(s) 671, 926, 1255
ErhI CCWWGG 3 cut(s) 671, 926, 1255
FaqI GGGAC 3 cut(s) 30, 421, 593
FauI CCCGC 1 cut(s) 679
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 2 cut(s) 730, 1038
Fnu4HI GCNGC 3 cut(s) 280, 964, 1217
FokI GGATG 1 cut(s) 1375
Fsp4HI GCNGC 3 cut(s) 280, 964, 1217
FspBI CTAG 5 cut(s) 38, 81, 647, 1184, 1245
GluI GCNGC 3 cut(s) 280, 964, 1217
HaeIII GGCC 2 cut(s) 483, 1254
HgaI GACGC 1 cut(s) 605
HindIII AAGCTT 2 cut(s) 59, 774
HinfI GANTC 9 cut(s) 46, 128, 512, 544, 642, 1119, 1261, 1309, 1337
HphI GGTGA 5 cut(s) 238, 344, 734, 1001, 1331
Hpy166II GTNNAC 6 cut(s) 262, 731, 910, 1039, 1156, 1241
Hpy188I TCNGA 6 cut(s) 133, 143, 288, 504, 781, 1081
Hpy8I GTNNAC 6 cut(s) 262, 731, 910, 1039, 1156, 1241
HpyAV CCTTC 5 cut(s) 194, 199, 719, 980, 1181
HpyCH4III ACNGT 3 cut(s) 256, 359, 565
HpyCH4IV ACGT 2 cut(s) 465, 1041
HpyCH4V TGCA 4 cut(s) 593, 973, 1100, 1125
HpyF10VI GCNNNNNNNGC 2 cut(s) 937, 1331
HpyF3I CTNAG 2 cut(s) 873, 918
HpySE526I ACGT 2 cut(s) 465, 1041
Kzo9I GATC 3 cut(s) 103, 193, 1076
LmnI GCTCC 3 cut(s) 169, 715, 1378
Lsp1109I GCAGC 3 cut(s) 291, 975, 1228
LweI GCATC 1 cut(s) 982
MabI ACCWGGT 1 cut(s) 241
MaeI CTAG 5 cut(s) 38, 81, 647, 1184, 1245
MaeII ACGT 2 cut(s) 465, 1041
MaeIII GTNAC 6 cut(s) 86, 226, 344, 1136, 1195, 1347
MalI GATC 3 cut(s) 105, 195, 1078
MboI GATC 3 cut(s) 103, 193, 1076
MboII GAAGA 2 cut(s) 553, 834
MfeI CAATTG 1 cut(s) 1164
MhlI GDGCHC 2 cut(s) 148, 1383
MlsI TGGCCA 1 cut(s) 1254
MluCI AATT 6 cut(s) 306, 331, 370, 699, 716, 1164
MluNI TGGCCA 1 cut(s) 1254
MlyI GAGTC 2 cut(s) 122, 1113
Mox20I TGGCCA 1 cut(s) 1254
MscI TGGCCA 1 cut(s) 1254
MslI CAYNNNNRTG 3 cut(s) 1062, 1068, 1202
Msp20I TGGCCA 1 cut(s) 1254
MspR9I CCNGG 2 cut(s) 153, 243
MunI CAATTG 1 cut(s) 1164
MvaI CCWGG 2 cut(s) 153, 243
MwoI GCNNNNNNNGC 2 cut(s) 937, 1331
NcoI CCATGG 2 cut(s) 926, 1255
NdeI CATATG 1 cut(s) 856
NdeII GATC 3 cut(s) 103, 193, 1076
NlaIV GGNNCC 2 cut(s) 385, 581
NmeAIII GCCGAG 1 cut(s) 1208
NmuCI GTSAC 3 cut(s) 226, 1136, 1195
NspI RCATGY 2 cut(s) 121, 1061
PagI TCATGA 3 cut(s) 196, 610, 694
PciI ACATGT 1 cut(s) 1057
PfeI GAWTC 7 cut(s) 46, 512, 544, 642, 1261, 1309, 1337
PflFI GACNNNGTC 1 cut(s) 1043
PkrI GCNGC 3 cut(s) 281, 965, 1218
PleI GAGTC 2 cut(s) 122, 1113
PpsI GAGTC 2 cut(s) 122, 1113
PpuMI RGGWCCY 1 cut(s) 580
PscI ACATGT 1 cut(s) 1057
PsiI TTATAA 3 cut(s) 159, 440, 1032
Psp5II RGGWCCY 1 cut(s) 580
Psp6I CCWGG 2 cut(s) 151, 241
PspEI GGTNACC 1 cut(s) 226
PspGI CCWGG 2 cut(s) 151, 241
PspN4I GGNNCC 2 cut(s) 385, 581
PspPI GGNCC 2 cut(s) 458, 580
PspPPI RGGWCCY 1 cut(s) 580
PsyI GACNNNGTC 1 cut(s) 1043
RsaI GTAC 5 cut(s) 42, 253, 361, 787, 803
RsaNI GTAC 5 cut(s) 41, 252, 360, 786, 802
RseI CAYNNNNRTG 3 cut(s) 1062, 1068, 1202
SatI GCNGC 3 cut(s) 280, 964, 1217
Sau3AI GATC 3 cut(s) 103, 193, 1076
Sau96I GGNCC 2 cut(s) 458, 580
ScaI AGTACT 2 cut(s) 42, 253
SchI GAGTC 2 cut(s) 122, 1113
ScrFI CCNGG 2 cut(s) 153, 243
SduI GDGCHC 2 cut(s) 148, 1383
SexAI ACCWGGT 1 cut(s) 241
SfaNI GCATC 1 cut(s) 982
SfcI CTRYAG 1 cut(s) 1394
SinI GGWCC 2 cut(s) 458, 580
SmiMI CAYNNNNRTG 3 cut(s) 1062, 1068, 1202
SmlI CTYRAG 3 cut(s) 91, 903, 1265
SmoI CTYRAG 3 cut(s) 91, 903, 1265
SpeI ACTAGT 2 cut(s) 37, 80
Sse9I AATT 6 cut(s) 306, 331, 370, 699, 716, 1164
SsiI CCGC 2 cut(s) 686, 1214
SspMI CTAG 5 cut(s) 38, 81, 647, 1184, 1245
StyD4I CCNGG 2 cut(s) 151, 241
StyI CCWWGG 3 cut(s) 671, 926, 1255
TaaI ACNGT 3 cut(s) 256, 359, 565
TaiI ACGT 2 cut(s) 468, 1044
TasI AATT 6 cut(s) 306, 331, 370, 699, 716, 1164
TatI WGTACW 3 cut(s) 40, 251, 785
TfiI GAWTC 7 cut(s) 46, 512, 544, 642, 1261, 1309, 1337
TscAI CASTG 1 cut(s) 362
TseFI GTSAC 3 cut(s) 226, 1136, 1195
TseI GCWGC 3 cut(s) 279, 963, 1216
Tsp45I GTSAC 3 cut(s) 226, 1136, 1195
TspDTI ATGAA 8 cut(s) 167, 213, 252, 414, 599, 711, 1052, 1086
TspRI CASTG 1 cut(s) 362
Tth111I GACNNNGTC 1 cut(s) 1043
VpaK11BI GGWCC 2 cut(s) 458, 580
XbaI TCTAGA 1 cut(s) 1183
XceI RCATGY 2 cut(s) 121, 1061
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XmiI GTMKAC 2 cut(s) 730, 1038
XspI CTAG 5 cut(s) 38, 81, 647, 1184, 1245
ZrmI AGTACT 2 cut(s) 42, 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.