RLG00000011210

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009780
Physical Location & Seq
Reverse (-)
7714256 .. 7716527
2272 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011210

Sequence Viewer

Length: 1407 bp
ATGGACTGCTCTACTGGGCTTGTAGCGGCTACCATACTAGTCCTGTTTCTTTCTGTCAAGCTTTGTGAAAGTGGGATCACCAGTAGTTACTTGAGGAACGATGACTTGGTCCATGATATGCCTTTAGACAGTGACGTGTTCCGAGCCCCTCCTGGGTATAATTCCCCTCAACAGGTTCATATAACACAAGGGGATCATGAGGGGAAGGGAGTGATTGTGTCTTGGGTGACTCCAAATGAACCTGGTTCAAGTACTGTGGTGTACTGGGCAGACAATAGTAGCAATCTGAAAAAGACTGCTTATGGCATTGTTCTTACTTACAAGTACTTCAATTACACCTCTGGTTACATTCATCACTGTACCATTGACAATTTGGAGTTTGACACCAAATACTATTATCAAGTTGGGATTGGGAATACCACGAGACGTTTTTGGTTTATAACCCCTCCTAAAGTTGGTCCTGATGTTCCCTACACTTTTGGCCTCATAGGGGATATTGGTCAAACCCCTGACTCAAATCGAACACTTACTCATTATGAGTTAAACCCAGCAAAAGGACAGACGGTGTTGTTCGTTGGGGACCTCTCTTATGCAGATGCTTATCCGTTTCATGACAATAGAAGATGGGATACATGGGGGAGATTCATAGAGAGAAATGCTGCCTATCAACCTTGGATTTGGACTGCGGGAAATCACGAAATTGATTTTGCTCCTCAATATGGTGAAAGTATACCATTCAAACCATATACAAATCGCTTTTTTGTTCCATACAAAGCTTCAGATAGTACATCTCCTCTCTGGTACTCCATCAAGAGAGCTTCAGCATATATCATAGTCTTGTCCTCTTACTCGGCATATGGAAAATATACTCCTCAGTATAAGTGGCTTGAAAAGGAGTTGCCTAAAGTGAACAGAACTGAGACACCATGGCTTATTGTTCTTGTGCATTGTCCAATGTATAGCAGCTATGTGCATCATTATATGGAAGGTGAAACCATGAGGGTTATGTATGAGCAATGGTTCGTGGATTATAAAGTAGATGTTGTCTTTGCCGGTCATGTTCATGCCTATGAACGATCTGAAAGGATATCAAACATTGCATACAACATTACAAATGGACTTTGCACTCCCATAAGTGACCAATCAGCGCCAGTTTACATAACAATTGGAGATGGAGGAAATCTAGAAGGATTGGTTACTGAAATGACAGAGCCGCAGCCAAGCTACTCAGCATTTCGTGAGGCTAGCTTTGGCCATGGGATTCTTGAGATAAAAAACAGAAGCTATGCCTTCGTCAGTTGGCATAGGAATCAAGATGGTTATGCAGTAGAAGCTGATTCTTTATGGTTACGCAACAGATATTGGAAAACTACTTTGGAACAGTCATCGTCCCTAACTGCGTCATAA

Protein Analysis

469

Amino Acids

53.51

Weight (kDa)

5.83

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pur_ac_phosph_N PF16656 56 - 148 1.7e-20 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 159 - 357 2.3e-26 Calcineurin-like phosphoesterase
Metallophos_C PF14008 383 - 442 3e-17 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009780)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G52940 AT2G27190
fragaria_vesca FvH4_2g38890 FvH4_2g38890 FvH4_2g38890 FvH4_2g38890 FvH4_2g38890 FvH4_2g38890
malus_domestica MD15G1020000.v1.1
prunus_persica Prupe.1G372000_v2.0.a1
pyrus_communis pycom15g01660
rosa_chinensis RchiOBHm_Chr6g0301991
rosa_laevigata RLG00000011210
rosa_multiflora Rmu_sc0006892.1_g000004
rosa_roxburghii Rroxscaffold_7G00166210
rosa_rugosa Rorug06G0310900
rosa_samantha Rh6AG423400 Rh6BG430100 Rh6CG436500 Rh6DG423500
rosa_wichuraiana Rw6G036680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 440, 1032
AccI GTMKAC 1 cut(s) 730
AciI CCGC 3 cut(s) 26, 686, 1214
AclWI GGATC 2 cut(s) 83, 201
AcoI YGGCCR 1 cut(s) 1252
AcuI CTGAAG 2 cut(s) 762, 804
AfaI GTAC 6 cut(s) 253, 263, 326, 361, 787, 803
AfiI CCNNNNNNNGG 6 cut(s) 153, 172, 455, 490, 554, 719
AflIII ACRYGT 1 cut(s) 135
AgsI TTSAA 4 cut(s) 249, 331, 739, 890
AhlI ACTAGT 1 cut(s) 37
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 2 cut(s) 151, 241
AjuI GAANNNNNNNTTGG 4 cut(s) 89, 121, 1358, 1390
AluBI AGCT 8 cut(s) 61, 776, 818, 966, 1224, 1248, 1284, 1334
AluI AGCT 8 cut(s) 61, 776, 818, 966, 1224, 1248, 1284, 1334
Alw26I GTCTC 2 cut(s) 418, 914
AlwI GGATC 2 cut(s) 83, 201
AoxI GGCC 2 cut(s) 481, 1252
ApeKI GCWGC 3 cut(s) 659, 963, 1216
AspLEI GCGC 1 cut(s) 1150
AspS9I GGNCC 3 cut(s) 109, 458, 580
AsuHPI GGTGA 4 cut(s) 70, 238, 734, 1001
AsuNHI GCTAGC 1 cut(s) 1244
AvaII GGWCC 3 cut(s) 109, 458, 580
BalI TGGCCA 1 cut(s) 1254
BanII GRGCYC 1 cut(s) 148
BarI GAAGNNNNNNTAC 2 cut(s) 613, 645
BauI CACGAG 1 cut(s) 421
BbvI GCAGC 3 cut(s) 646, 975, 1228
BccI CCATC 4 cut(s) 618, 815, 1166, 1310
BciT130I CCWGG 2 cut(s) 153, 243
BciVI GTATCC 1 cut(s) 622
BcoDI GTCTC 2 cut(s) 418, 914
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 3 cut(s) 38, 1184, 1245
BfoI RGCGCY 1 cut(s) 1151
BfuI GTATCC 1 cut(s) 622
BisI GCNGC 5 cut(s) 27, 660, 964, 1214, 1217
BlsI GCNGC 5 cut(s) 28, 661, 965, 1215, 1218
BmcAI AGTACT 2 cut(s) 253, 326
Bme1390I CCNGG 2 cut(s) 153, 243
Bme18I GGWCC 3 cut(s) 109, 458, 580
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 3 cut(s) 109, 458, 580
BmiI GGNNCC 1 cut(s) 581
BmrFI CCNGG 2 cut(s) 153, 243
BmrI ACTGGG 2 cut(s) 24, 274
BmsI GCATC 2 cut(s) 586, 982
BmtI GCTAGC 1 cut(s) 1248
BmuI ACTGGG 2 cut(s) 24, 274
BpuEI CTTGAG 2 cut(s) 112, 1286
BsaBI GATNNNNATC 1 cut(s) 600
BsaJI CCNNGG 4 cut(s) 152, 671, 926, 1255
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 6 cut(s) 153, 172, 455, 490, 554, 719
Bse118I RCCGGY 1 cut(s) 1052
Bse1I ACTGG 4 cut(s) 19, 81, 269, 1151
Bse3DI GCAATG 2 cut(s) 1022, 1095
Bse8I GATNNNNATC 1 cut(s) 600
BseBI CCWGG 2 cut(s) 153, 243
BseDI CCNNGG 4 cut(s) 152, 671, 926, 1255
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 6 cut(s) 153, 172, 455, 490, 554, 719
BseMI GCAATG 2 cut(s) 1022, 1095
BseMII CTCAG 3 cut(s) 887, 909, 1242
BseNI ACTGG 4 cut(s) 19, 81, 269, 1151
BseRI GAGGAG 3 cut(s) 702, 783, 861
BseXI GCAGC 3 cut(s) 646, 975, 1228
BseYI CCCAGC 1 cut(s) 547
BshFI GGCC 2 cut(s) 483, 1254
BsiSI CCGG 1 cut(s) 1053
BslFI GGGAC 2 cut(s) 593, 1375
BslI CCNNNNNNNGG 6 cut(s) 153, 172, 455, 490, 554, 719
BsmAI GTCTC 2 cut(s) 418, 914
BsmBI CGTCTC 1 cut(s) 418
BsmFI GGGAC 2 cut(s) 593, 1375
BsnI GGCC 2 cut(s) 483, 1254
Bsp1286I GDGCHC 1 cut(s) 148
Bsp143I GATC 3 cut(s) 75, 193, 1076
Bsp19I CCATGG 2 cut(s) 926, 1255
BspACI CCGC 3 cut(s) 26, 686, 1214
BspANI GGCC 2 cut(s) 483, 1254
BspCNI CTCAG 3 cut(s) 886, 910, 1241
BspHI TCATGA 2 cut(s) 196, 610
BspLI GGNNCC 1 cut(s) 581
BspOI GCTAGC 1 cut(s) 1248
BspPI GGATC 2 cut(s) 83, 201
BsrDI GCAATG 2 cut(s) 1022, 1095
BsrFI RCCGGY 1 cut(s) 1052
BsrI ACTGG 4 cut(s) 19, 81, 269, 1151
BssAI RCCGGY 1 cut(s) 1052
BssECI CCNNGG 4 cut(s) 152, 671, 926, 1255
BssMI GATC 3 cut(s) 75, 193, 1076
BssNAI GTATAC 1 cut(s) 731
BssSI CACGAG 1 cut(s) 421
BssT1I CCWWGG 3 cut(s) 671, 926, 1255
Bst1107I GTATAC 1 cut(s) 731
Bst2BI CACGAG 1 cut(s) 421
Bst2UI CCWGG 2 cut(s) 153, 243
Bst4CI ACNGT 5 cut(s) 131, 256, 359, 565, 1383
BstC8I GCNNGC 1 cut(s) 1246
BstDEI CTNAG 3 cut(s) 873, 918, 1228
BstDSI CCRYGG 2 cut(s) 926, 1255
BstH2I RGCGCY 1 cut(s) 1151
BstHHI GCGC 1 cut(s) 1150
BstKTI GATC 3 cut(s) 78, 196, 1079
BstMAI GTCTC 2 cut(s) 418, 914
BstMBI GATC 3 cut(s) 75, 193, 1076
BstMWI GCNNNNNNNGC 1 cut(s) 1331
BstNI CCWGG 2 cut(s) 153, 243
BstSCI CCNGG 2 cut(s) 151, 241
BstV1I GCAGC 3 cut(s) 646, 975, 1228
BstZ17I GTATAC 1 cut(s) 731
BsuI GTATCC 1 cut(s) 622
BsuRI GGCC 2 cut(s) 483, 1254
BtgI CCRYGG 2 cut(s) 926, 1255
BtrI CACGTC 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 136, 355
Cac8I GCNNGC 1 cut(s) 1246
CciI TCATGA 2 cut(s) 196, 610
CfoI GCGC 1 cut(s) 1150
Cfr10I RCCGGY 1 cut(s) 1052
Cfr13I GGNCC 3 cut(s) 109, 458, 580
CseI GACGC 1 cut(s) 1389
CsiI ACCWGGT 1 cut(s) 241
Csp6I GTAC 6 cut(s) 252, 262, 325, 360, 786, 802
CspCI CAANNNNNGTGG 2 cut(s) 237, 272
CviAII CATG 9 cut(s) 113, 197, 611, 633, 927, 997, 1058, 1064, 1256
CviQI GTAC 6 cut(s) 252, 262, 325, 360, 786, 802
DdeI CTNAG 3 cut(s) 873, 918, 1228
DpnI GATC 3 cut(s) 77, 195, 1078
DpnII GATC 3 cut(s) 75, 193, 1076
EaeI YGGCCR 1 cut(s) 1252
Eco130I CCWWGG 3 cut(s) 671, 926, 1255
Eco24I GRGCYC 1 cut(s) 148
Eco32I GATATC 1 cut(s) 1089
Eco47I GGWCC 3 cut(s) 109, 458, 580
Eco57I CTGAAG 2 cut(s) 762, 804
EcoO109I RGGNCCY 1 cut(s) 580
EcoRII CCWGG 2 cut(s) 151, 241
EcoRV GATATC 1 cut(s) 1089
EcoT14I CCWWGG 3 cut(s) 671, 926, 1255
EcoT38I GRGCYC 1 cut(s) 148
ErhI CCWWGG 3 cut(s) 671, 926, 1255
Esp3I CGTCTC 1 cut(s) 418
FaeI CATG 9 cut(s) 116, 200, 614, 636, 930, 1000, 1061, 1067, 1259
FaqI GGGAC 2 cut(s) 593, 1375
FatI CATG 9 cut(s) 112, 196, 610, 632, 926, 996, 1057, 1063, 1255
FauI CCCGC 1 cut(s) 679
FauNDI CATATG 1 cut(s) 856
FblI GTMKAC 1 cut(s) 730
Fnu4HI GCNGC 5 cut(s) 27, 660, 964, 1214, 1217
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 5 cut(s) 27, 660, 964, 1214, 1217
FspBI CTAG 3 cut(s) 38, 1184, 1245
GlaI GCGC 1 cut(s) 1149
GluI GCNGC 5 cut(s) 27, 660, 964, 1214, 1217
GsaI CCCAGC 1 cut(s) 551
HaeII RGCGCY 1 cut(s) 1151
HaeIII GGCC 2 cut(s) 483, 1254
HapII CCGG 1 cut(s) 1053
HgaI GACGC 1 cut(s) 1389
HhaI GCGC 1 cut(s) 1150
Hin1II CATG 9 cut(s) 116, 200, 614, 636, 930, 1000, 1061, 1067, 1259
Hin6I GCGC 1 cut(s) 1148
HinP1I GCGC 1 cut(s) 1148
HindIII AAGCTT 2 cut(s) 59, 774
HinfI GANTC 6 cut(s) 229, 512, 642, 1261, 1309, 1337
HpaII CCGG 1 cut(s) 1053
HphI GGTGA 4 cut(s) 70, 238, 734, 1001
Hpy166II GTNNAC 4 cut(s) 262, 731, 910, 1156
Hpy188I TCNGA 4 cut(s) 143, 288, 781, 1081
Hpy188III TCNNGA 9 cut(s) 197, 461, 611, 695, 811, 1184, 1238, 1265, 1313
Hpy8I GTNNAC 4 cut(s) 262, 731, 910, 1156
HpyAV CCTTC 4 cut(s) 199, 980, 1181, 1300
HpyCH4III ACNGT 5 cut(s) 131, 256, 359, 565, 1383
HpyCH4IV ACGT 2 cut(s) 135, 427
HpyCH4V TGCA 6 cut(s) 593, 946, 973, 1100, 1125, 1325
HpyF10VI GCNNNNNNNGC 1 cut(s) 1331
HpyF3I CTNAG 3 cut(s) 873, 918, 1228
HpySE526I ACGT 2 cut(s) 135, 427
Hsp92II CATG 9 cut(s) 116, 200, 614, 636, 930, 1000, 1061, 1067, 1259
HspAI GCGC 1 cut(s) 1148
Kzo9I GATC 3 cut(s) 75, 193, 1076
LmnI GCTCC 1 cut(s) 715
Lsp1109I GCAGC 3 cut(s) 646, 975, 1228
LweI GCATC 2 cut(s) 586, 982
MabI ACCWGGT 1 cut(s) 241
MaeI CTAG 3 cut(s) 38, 1184, 1245
MaeII ACGT 2 cut(s) 135, 427
MaeIII GTNAC 7 cut(s) 86, 131, 226, 344, 1136, 1195, 1347
MalI GATC 3 cut(s) 77, 195, 1078
MboI GATC 3 cut(s) 75, 193, 1076
MboII GAAGA 1 cut(s) 633
MfeI CAATTG 1 cut(s) 1164
MhlI GDGCHC 1 cut(s) 148
MlsI TGGCCA 1 cut(s) 1254
MluCI AATT 5 cut(s) 160, 331, 370, 699, 1164
MluNI TGGCCA 1 cut(s) 1254
MlyI GAGTC 2 cut(s) 223, 506
Mox20I TGGCCA 1 cut(s) 1254
MscI TGGCCA 1 cut(s) 1254
MseI TTAA 1 cut(s) 542
MslI CAYNNNNRTG 2 cut(s) 1062, 1068
Msp20I TGGCCA 1 cut(s) 1254
MspI CCGG 1 cut(s) 1053
MspR9I CCNGG 2 cut(s) 153, 243
MunI CAATTG 1 cut(s) 1164
MvaI CCWGG 2 cut(s) 153, 243
MwoI GCNNNNNNNGC 1 cut(s) 1331
NcoI CCATGG 2 cut(s) 926, 1255
NdeI CATATG 1 cut(s) 856
NdeII GATC 3 cut(s) 75, 193, 1076
NheI GCTAGC 1 cut(s) 1244
NlaIII CATG 9 cut(s) 116, 200, 614, 636, 930, 1000, 1061, 1067, 1259
NlaIV GGNNCC 1 cut(s) 581
NmeAIII GCCGAG 1 cut(s) 830
NmuCI GTSAC 3 cut(s) 131, 226, 1136
PagI TCATGA 2 cut(s) 196, 610
PfeI GAWTC 4 cut(s) 642, 1261, 1309, 1337
PflFI GACNNNGTC 1 cut(s) 107
PkrI GCNGC 5 cut(s) 28, 661, 965, 1215, 1218
PleI GAGTC 2 cut(s) 223, 506
PpsI GAGTC 2 cut(s) 223, 506
PpuMI RGGWCCY 1 cut(s) 580
PsiI TTATAA 2 cut(s) 440, 1032
Psp5II RGGWCCY 1 cut(s) 580
Psp6I CCWGG 2 cut(s) 151, 241
PspFI CCCAGC 1 cut(s) 547
PspGI CCWGG 2 cut(s) 151, 241
PspN4I GGNNCC 1 cut(s) 581
PspPI GGNCC 3 cut(s) 109, 458, 580
PspPPI RGGWCCY 1 cut(s) 580
PsyI GACNNNGTC 1 cut(s) 107
RsaI GTAC 6 cut(s) 253, 263, 326, 361, 787, 803
RsaNI GTAC 6 cut(s) 252, 262, 325, 360, 786, 802
RseI CAYNNNNRTG 2 cut(s) 1062, 1068
SaqAI TTAA 1 cut(s) 542
SatI GCNGC 5 cut(s) 27, 660, 964, 1214, 1217
Sau3AI GATC 3 cut(s) 75, 193, 1076
Sau96I GGNCC 3 cut(s) 109, 458, 580
ScaI AGTACT 2 cut(s) 253, 326
SchI GAGTC 2 cut(s) 223, 506
ScrFI CCNGG 2 cut(s) 153, 243
SduI GDGCHC 1 cut(s) 148
SexAI ACCWGGT 1 cut(s) 241
SfaNI GCATC 2 cut(s) 586, 982
SinI GGWCC 3 cut(s) 109, 458, 580
SmiMI CAYNNNNRTG 2 cut(s) 1062, 1068
SmlI CTYRAG 2 cut(s) 91, 1265
SmoI CTYRAG 2 cut(s) 91, 1265
SpeI ACTAGT 1 cut(s) 37
Sse9I AATT 5 cut(s) 160, 331, 370, 699, 1164
SsiI CCGC 3 cut(s) 26, 686, 1214
SspMI CTAG 3 cut(s) 38, 1184, 1245
StyD4I CCNGG 2 cut(s) 151, 241
StyI CCWWGG 3 cut(s) 671, 926, 1255
TaaI ACNGT 5 cut(s) 131, 256, 359, 565, 1383
TaiI ACGT 2 cut(s) 138, 430
TaqI TCGA 1 cut(s) 520
TasI AATT 5 cut(s) 160, 331, 370, 699, 1164
TatI WGTACW 4 cut(s) 251, 261, 324, 785
TauI GCSGC 2 cut(s) 29, 1216
TfiI GAWTC 4 cut(s) 642, 1261, 1309, 1337
Tru1I TTAA 1 cut(s) 542
Tru9I TTAA 1 cut(s) 542
TscAI CASTG 2 cut(s) 136, 362
TseFI GTSAC 3 cut(s) 131, 226, 1136
TseI GCWGC 3 cut(s) 659, 963, 1216
Tsp45I GTSAC 3 cut(s) 131, 226, 1136
TspDTI ATGAA 7 cut(s) 167, 252, 341, 599, 634, 1052, 1086
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 2 cut(s) 136, 362
Tth111I GACNNNGTC 1 cut(s) 107
VpaK11BI GGWCC 3 cut(s) 109, 458, 580
XbaI TCTAGA 1 cut(s) 1183
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 730
XspI CTAG 3 cut(s) 38, 1184, 1245
ZrmI AGTACT 2 cut(s) 253, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.