FvH4_2g38950

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012802
Physical Location & Seq
Forward (+)
28080389 .. 28082959
2571 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38950.t1

Sequence Viewer

Length: 1035 bp
ATGGCTGGGTTCTTCGGTAGCTTAGGGGGAGGAAGAGATCAGGGGGTTAGAAACAAACAAGGTGGAGACGATGAAAGAGGAGGAAACTTAAACCTGTTTTCGTACGGAAACGAAGAGATCTACAACAATTCCAAGGGCGGATTCGAGTTATGGCCACAGTATCATCCTCACCATCCTCATCATCATCATCATCAGCAGCAAAGCTTGAACTACTACGCGTTTGGAGTGGATCCTAGCCGTAGAAGCAACCACAACAACTTGGAGGGCAACGTCTCGGATGACTCATCTTCGGGGCTGAGACTCACTGTGATGCGGCAAGGAAGCGGTACCGGTGGCGGTGGTGGTAGTGGTACTGGTCTAGGATCGGGAGGAATGAATTGTCAGGATTGCGGGAATCAAGCTAAGAAAGACTGTCCTCACTTGCGGTGCCGAACCTGTTGCAAGAGCCGAGGGTTTCAGTGCCAAACACACGTCAAGAGCACTTGGGTACCTGCAGCCAAACGCCGAGAGAGGCAGGAACAATTCTCTGCTCTTCAGCAGCAGCAACAACAACAACAATTGCAATTTCCGAAAAGGCAGAGAGAGAATCAAGCCGGAGGCTCATCTCTAGCCTGCGTTCGTTTACCCACTAACACGTCAGGGTTGGAGCTTGCACCATTTCCAGCGGAGGTGAGTTCACCAGCCGTGTTCCGGTGTGTGAGAGTCAGCGCAATGGACGATGTGGATGAGCAGTACGCGTACCAGACGGCTGTGAACATCGGAGGCCACGTGTTCAAGGGACTTCTCTACGATCATGGGCTGGACAATGCTAATTACACAAGCGCAACCACCGTCGGCGAAAGCTCCTCAGGCGGCCAACAAGACGGAGGAGGAGGGCATCATAATAATCAACAGCAACTGAATCTCATAACCGCCCCTACAAGCACAAGTGCCAACCCTCCTACGGCGACGTTGCTCGATCCTACTATGGTGTTTCCGACTCCGCTCAATGCTTTTGTGGCTGGTACGCAATACTTCCCACCTCCGAGGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

345

Amino Acids

37.14

Weight (kDa)

7.67

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 124 - 268 2.5e-64 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19790 AT1G19790 AT1G75520
fragaria_vesca FvH4_2g38950
malus_domestica MD08G1021500.v1.1 MD15G1019200.v1.1
prunus_persica Prupe.1G371500_v2.0.a1 Prupe.1G371500_v2.0.a1
pyrus_communis pycom08g01700 pycom15g01600
rosa_chinensis RchiOBHm_Chr6g0302041
rosa_laevigata RLG00000011200
rosa_multiflora Rmu_ssc0000160.1_g000001
rosa_roxburghii Rroxscaffold_7G00166150
rosa_rugosa Rorug06G0311300
rosa_samantha Rh6AG424100 Rh6CG437100
rosa_wichuraiana Rw6G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 499
Acc65I GGTACC 2 cut(s) 326, 487
AccB1I GGYRCC 3 cut(s) 326, 426, 487
AccBSI CCGCTC 1 cut(s) 985
AccII CGCG 2 cut(s) 218, 737
AclWI GGATC 4 cut(s) 224, 237, 370, 953
AcoI YGGCCR 2 cut(s) 152, 853
AcuI CTGAAG 1 cut(s) 518
AcvI CACGTG 1 cut(s) 769
AfaI GTAC 7 cut(s) 104, 328, 352, 489, 734, 740, 1006
AfiI CCNNNNNNNGG 2 cut(s) 690, 943
AflIII ACRYGT 5 cut(s) 216, 469, 633, 735, 768
AgeI ACCGGT 1 cut(s) 329
AgsI TTSAA 2 cut(s) 208, 775
AjiI CACGTC 2 cut(s) 472, 636
AjuI GAANNNNNNNTTGG 2 cut(s) 125, 157
AluBI AGCT 5 cut(s) 21, 204, 401, 649, 843
AluI AGCT 5 cut(s) 21, 204, 401, 649, 843
Alw21I GWGCWC 1 cut(s) 482
Alw26I GTCTC 3 cut(s) 60, 277, 292
AlwI GGATC 4 cut(s) 224, 237, 370, 953
AlwNI CAGNNNCTG 1 cut(s) 898
AoxI GGCC 3 cut(s) 152, 763, 853
ApeKI GCWGC 4 cut(s) 196, 494, 538, 541
AsiGI ACCGGT 1 cut(s) 329
Asp718I GGTACC 2 cut(s) 326, 487
AspLEI GCGC 2 cut(s) 710, 824
AsuHPI GGTGA 3 cut(s) 161, 669, 682
AxyI CCTNAGG 1 cut(s) 847
BaeI ACNNNNGTAYC 2 cut(s) 471, 504
BalI TGGCCA 1 cut(s) 154
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 3 cut(s) 326, 426, 487
BbrPI CACGTG 1 cut(s) 769
Bbv12I GWGCWC 1 cut(s) 482
BbvI GCAGC 4 cut(s) 208, 506, 550, 553
BccI CCATC 1 cut(s) 180
BceAI ACGGC 4 cut(s) 222, 668, 762, 960
BcgI CGANNNNNNTGC 2 cut(s) 420, 454
BcoDI GTCTC 3 cut(s) 60, 277, 292
BfaI CTAG 3 cut(s) 234, 359, 608
BfmI CTRYAG 1 cut(s) 492
BfuAI ACCTGC 1 cut(s) 499
BglII AGATCT 1 cut(s) 117
BisI GCNGC 6 cut(s) 197, 314, 495, 539, 542, 853
BlsI GCNGC 6 cut(s) 198, 315, 496, 540, 543, 854
BmgBI CACGTC 2 cut(s) 472, 636
BmiI GGNNCC 4 cut(s) 231, 328, 428, 489
BmsI GCATC 2 cut(s) 300, 886
Bpu10I CCTNAGC 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 769
BsaJI CCNNGG 3 cut(s) 132, 448, 1025
BsaWI WCCGGW 2 cut(s) 329, 690
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bsc4I CCNNNNNNNGG 2 cut(s) 690, 943
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 1 cut(s) 358
Bse21I CCTNAGG 1 cut(s) 847
Bse3DI GCAATG 1 cut(s) 717
BseDI CCNNGG 3 cut(s) 132, 448, 1025
BseGI GGATG 4 cut(s) 163, 172, 283, 730
BseLI CCNNNNNNNGG 2 cut(s) 690, 943
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 2 cut(s) 287, 861
BseNI ACTGG 1 cut(s) 358
BseRI GAGGAG 4 cut(s) 93, 835, 882, 885
BseXI GCAGC 4 cut(s) 208, 506, 550, 553
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 2 cut(s) 218, 737
BshFI GGCC 3 cut(s) 154, 765, 855
BshNI GGYRCC 3 cut(s) 326, 426, 487
BshTI ACCGGT 1 cut(s) 329
BsiHKAI GWGCWC 1 cut(s) 482
BsiSI CCGG 3 cut(s) 330, 594, 691
BsiWI CGTACG 1 cut(s) 102
BslFI GGGAC 1 cut(s) 792
BslI CCNNNNNNNGG 2 cut(s) 690, 943
BsmAI GTCTC 3 cut(s) 60, 277, 292
BsmBI CGTCTC 2 cut(s) 60, 277
BsmFI GGGAC 1 cut(s) 792
BsnI GGCC 3 cut(s) 154, 765, 855
Bsp1286I GDGCHC 1 cut(s) 482
Bsp143I GATC 6 cut(s) 37, 117, 229, 362, 790, 958
BspANI GGCC 3 cut(s) 154, 765, 855
BspCNI CTCAG 2 cut(s) 288, 860
BspFNI CGCG 2 cut(s) 218, 737
BspLI GGNNCC 4 cut(s) 231, 328, 428, 489
BspMAI CTGCAG 1 cut(s) 496
BspMI ACCTGC 1 cut(s) 499
BspPI GGATC 4 cut(s) 224, 237, 370, 953
BspQI GCTCTTC 1 cut(s) 537
BspT107I GGYRCC 3 cut(s) 326, 426, 487
BsrBI CCGCTC 1 cut(s) 985
BsrDI GCAATG 1 cut(s) 717
BsrFI RCCGGY 1 cut(s) 329
BsrI ACTGG 1 cut(s) 358
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 3 cut(s) 132, 448, 1025
BssMI GATC 6 cut(s) 37, 117, 229, 362, 790, 958
BssT1I CCWWGG 1 cut(s) 132
Bst4CI ACNGT 4 cut(s) 159, 307, 413, 832
Bst6I CTCTTC 3 cut(s) 28, 108, 537
BstBAI YACGTR 1 cut(s) 769
BstC8I GCNNGC 2 cut(s) 613, 651
BstDEI CTNAG 5 cut(s) 22, 296, 402, 847, 1032
BstF5I GGATG 4 cut(s) 163, 172, 283, 730
BstFNI CGCG 2 cut(s) 218, 737
BstHHI GCGC 2 cut(s) 710, 824
BstKTI GATC 6 cut(s) 40, 120, 232, 365, 793, 961
BstMAI GTCTC 3 cut(s) 60, 277, 292
BstMBI GATC 6 cut(s) 37, 117, 229, 362, 790, 958
BstMWI GCNNNNNNNGC 3 cut(s) 243, 849, 998
BstSFI CTRYAG 1 cut(s) 492
BstUI CGCG 2 cut(s) 218, 737
BstV1I GCAGC 4 cut(s) 208, 506, 550, 553
BstX2I RGATCY 2 cut(s) 117, 229
BstYI RGATCY 2 cut(s) 117, 229
Bsu36I CCTNAGG 1 cut(s) 847
BsuRI GGCC 3 cut(s) 154, 765, 855
BtrI CACGTC 2 cut(s) 472, 636
BtsCI GGATG 4 cut(s) 163, 172, 283, 730
BtsIMutI CAGTG 2 cut(s) 303, 464
BveI ACCTGC 1 cut(s) 499
Cac8I GCNNGC 2 cut(s) 613, 651
CaiI CAGNNNCTG 1 cut(s) 898
CfoI GCGC 2 cut(s) 710, 824
Cfr10I RCCGGY 1 cut(s) 329
Csp6I GTAC 7 cut(s) 103, 327, 351, 488, 733, 739, 1005
CspAI ACCGGT 1 cut(s) 329
CspCI CAANNNNNGTGG 2 cut(s) 43, 78
CviAII CATG 1 cut(s) 794
CviQI GTAC 7 cut(s) 103, 327, 351, 488, 733, 739, 1005
DdeI CTNAG 5 cut(s) 22, 296, 402, 847, 1032
DpnI GATC 6 cut(s) 39, 119, 231, 364, 792, 960
DpnII GATC 6 cut(s) 37, 117, 229, 362, 790, 958
EaeI YGGCCR 2 cut(s) 152, 853
Eam1104I CTCTTC 3 cut(s) 28, 108, 537
EarI CTCTTC 3 cut(s) 28, 108, 537
EciI GGCGGA 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 518
Eco72I CACGTG 1 cut(s) 769
Eco81I CCTNAGG 1 cut(s) 847
EcoT14I CCWWGG 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 132
Esp3I CGTCTC 2 cut(s) 60, 277
FaeI CATG 1 cut(s) 797
FaiI YATR 5 cut(s) 151, 795, 882, 908, 968
FaqI GGGAC 1 cut(s) 792
FatI CATG 1 cut(s) 793
FauI CCCGC 1 cut(s) 383
Fnu4HI GCNGC 6 cut(s) 197, 314, 495, 539, 542, 853
FokI GGATG 4 cut(s) 150, 159, 290, 737
Fsp4HI GCNGC 6 cut(s) 197, 314, 495, 539, 542, 853
FspBI CTAG 3 cut(s) 234, 359, 608
GlaI GCGC 2 cut(s) 709, 823
GluI GCNGC 6 cut(s) 197, 314, 495, 539, 542, 853
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 3 cut(s) 154, 765, 855
HapII CCGG 3 cut(s) 330, 594, 691
HhaI GCGC 2 cut(s) 710, 824
Hin1II CATG 1 cut(s) 797
Hin6I GCGC 2 cut(s) 708, 822
HinP1I GCGC 2 cut(s) 708, 822
HindIII AAGCTT 1 cut(s) 202
HinfI GANTC 8 cut(s) 141, 281, 300, 394, 586, 702, 901, 979
HpaII CCGG 3 cut(s) 330, 594, 691
HphI GGTGA 3 cut(s) 161, 669, 682
Hpy166II GTNNAC 3 cut(s) 623, 677, 754
Hpy188I TCNGA 5 cut(s) 277, 570, 761, 978, 1026
Hpy188III TCNNGA 3 cut(s) 366, 383, 475
Hpy8I GTNNAC 3 cut(s) 623, 677, 754
Hpy99I CGWCG 2 cut(s) 836, 952
HpyCH4III ACNGT 4 cut(s) 159, 307, 413, 832
HpyCH4IV ACGT 5 cut(s) 270, 471, 635, 768, 950
HpyCH4V TGCA 4 cut(s) 441, 494, 562, 653
HpyF10VI GCNNNNNNNGC 3 cut(s) 243, 849, 998
HpyF3I CTNAG 5 cut(s) 22, 296, 402, 847, 1032
HpySE526I ACGT 5 cut(s) 270, 471, 635, 768, 950
Hsp92II CATG 1 cut(s) 797
HspAI GCGC 2 cut(s) 708, 822
KpnI GGTACC 2 cut(s) 330, 491
Kzo9I GATC 6 cut(s) 37, 117, 229, 362, 790, 958
LguI GCTCTTC 1 cut(s) 537
LmnI GCTCC 2 cut(s) 646, 848
Lsp1109I GCAGC 4 cut(s) 208, 506, 550, 553
LweI GCATC 2 cut(s) 300, 886
MaeI CTAG 3 cut(s) 234, 359, 608
MaeII ACGT 5 cut(s) 270, 471, 635, 768, 950
MalI GATC 6 cut(s) 39, 119, 231, 364, 792, 960
MbiI CCGCTC 1 cut(s) 985
MboI GATC 6 cut(s) 37, 117, 229, 362, 790, 958
MboII GAAGA 5 cut(s) 4, 45, 125, 279, 524
MfeI CAATTG 1 cut(s) 557
MflI RGATCY 2 cut(s) 117, 229
MhlI GDGCHC 1 cut(s) 482
MlsI TGGCCA 1 cut(s) 154
MluCI AATT 6 cut(s) 127, 376, 521, 557, 563, 811
MluI ACGCGT 2 cut(s) 216, 735
MluNI TGGCCA 1 cut(s) 154
MlyI GAGTC 4 cut(s) 275, 294, 711, 973
MmeI TCCRAC 2 cut(s) 624, 1001
Mox20I TGGCCA 1 cut(s) 154
MscI TGGCCA 1 cut(s) 154
MseI TTAA 1 cut(s) 89
MslI CAYNNNNRTG 1 cut(s) 308
Msp20I TGGCCA 1 cut(s) 154
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 3 cut(s) 330, 594, 691
MunI CAATTG 1 cut(s) 557
MvnI CGCG 2 cut(s) 218, 737
MwoI GCNNNNNNNGC 3 cut(s) 243, 849, 998
NdeII GATC 6 cut(s) 37, 117, 229, 362, 790, 958
NlaIII CATG 1 cut(s) 797
NlaIV GGNNCC 4 cut(s) 231, 328, 428, 489
NmeAIII GCCGAG 2 cut(s) 473, 530
PciSI GCTCTTC 1 cut(s) 537
PcsI WCGNNNNNNNCGW 1 cut(s) 765
PfeI GAWTC 4 cut(s) 141, 394, 586, 901
Pfl23II CGTACG 1 cut(s) 102
PinAI ACCGGT 1 cut(s) 329
PkrI GCNGC 6 cut(s) 198, 315, 496, 540, 543, 854
PleI GAGTC 4 cut(s) 275, 294, 710, 973
PmaCI CACGTG 1 cut(s) 769
PmlI CACGTG 1 cut(s) 769
PpsI GAGTC 4 cut(s) 275, 294, 710, 973
Ppu21I YACGTR 1 cut(s) 769
PspCI CACGTG 1 cut(s) 769
PspFI CCCAGC 1 cut(s) 5
PspLI CGTACG 1 cut(s) 102
PspN4I GGNNCC 4 cut(s) 231, 328, 428, 489
PstI CTGCAG 1 cut(s) 496
PstNI CAGNNNCTG 1 cut(s) 898
PsuI RGATCY 2 cut(s) 117, 229
RsaI GTAC 7 cut(s) 104, 328, 352, 489, 734, 740, 1006
RsaNI GTAC 7 cut(s) 103, 327, 351, 488, 733, 739, 1005
RseI CAYNNNNRTG 1 cut(s) 308
SapI GCTCTTC 1 cut(s) 537
SaqAI TTAA 1 cut(s) 89
SatI GCNGC 6 cut(s) 197, 314, 495, 539, 542, 853
Sau3AI GATC 6 cut(s) 37, 117, 229, 362, 790, 958
SchI GAGTC 4 cut(s) 275, 294, 711, 973
SduI GDGCHC 1 cut(s) 482
SfaNI GCATC 2 cut(s) 300, 886
SfcI CTRYAG 1 cut(s) 492
SmiMI CAYNNNNRTG 1 cut(s) 308
Sse9I AATT 6 cut(s) 127, 376, 521, 557, 563, 811
SspMI CTAG 3 cut(s) 234, 359, 608
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 4 cut(s) 159, 307, 413, 832
TaiI ACGT 5 cut(s) 273, 474, 638, 771, 953
TaqI TCGA 2 cut(s) 144, 957
TasI AATT 6 cut(s) 127, 376, 521, 557, 563, 811
TauI GCSGC 2 cut(s) 316, 855
TfiI GAWTC 4 cut(s) 141, 394, 586, 901
Tru1I TTAA 1 cut(s) 89
Tru9I TTAA 1 cut(s) 89
TscAI CASTG 2 cut(s) 310, 464
TseI GCWGC 4 cut(s) 196, 494, 538, 541
TspDTI ATGAA 2 cut(s) 87, 389
TspGWI ACGGA 2 cut(s) 120, 879
TspRI CASTG 2 cut(s) 310, 464
XspI CTAG 3 cut(s) 234, 359, 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.