MD15G1019200.v1.1

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012802
Physical Location & Seq
Reverse (-)
1113803 .. 1115731
1929 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1019200.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGCTGGCTTGTTTTGCTTAGGAGGGAGAGATCAAAGTCCGAATAGCCGCGGCAAACAAGAGGGAGGGAATCAGAGCGGAAGAGTGGGAGGAGTAGCAGCGGAAGCGGCGGCTGGAGGAGGAGGAGGAGGGAACTTGTTTTTGTACAGATCAACAGGCGAGCCGCAGGCCGAGATCTACAACAAGGGGTTTGAGATATGGCCGTCACAATACCATCCACACCAAAACTTGAACTACTACTCGTTTGGAGTGGATCCTAGCTACAACAGTAGACACTTGGACAACAATAACGACGGCGTTTCGGCGGATGATCTATCGGCAGGGTTGAGGCTGACGGTGATGAGGGGAGGAGGGTTAGGAAGCAGCGGCGGCGGCATGAACTGTCAAGACTGCGGGAACCAAGCGAAGAAAGACTGTCCGCACCTTAGGTGCCGGACCTGTTGCAAGAGCCGAGGGTTTCAGTGTCAAACACACGTGAAGAGCACGTGGGTTCCCGCCGCGAAACGACGGGAGCGGCAGGAACAATTCTCGGCCTTACAACAAAACCGAAGTCAGCAACAACAGCAAGAGCAGCAGATACAGTATCGAGGTGAAAACCCCAAAAGGCAGAGAGATAACTCTCTTGCCTGCGTTCGTATTCCTTCCGACACGTCAGGGTTGGAGTTGAATCCATTTCCGCCAGAAGTGAGCTCACCGGCGGTGTTCCGCTGCGTAAAAGTAAGTGCAATGGACGATGTAGATGAGCAATTCGCGTATCAAACGGCTGTCAACATCGGAGGGCATGTGTTCAAGGGACTTCTGTACGATCAAGGCATGGATGGTCAGTATTACCCGAGTTCTGGAGGAGGAGGTGAGAGCTCCTCGGGCGGTCAAGGTACTAGCCACGATCATCACCAAGACGGAGGAGGAAGCGGAGGAGGAGGTCATCCACATCATCATCAGCAACATAATCTTGTAACAGTCGCGGCTGCCGGCGGGACCTCTGGCAATCCGTCTACTACGTTGCTTGACCCTTCTCTCTTCCCAGCTCCATTCAATGCTTTCATGGCTGGTACGCAATACTTTCCGCCTCCCAGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

361

Amino Acids

38.42

Weight (kDa)

6.91

Isoelectric Point (pI)

55.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 125 - 273 1e-65 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19790 AT1G19790 AT1G75520
fragaria_vesca FvH4_2g38950
malus_domestica MD08G1021500.v1.1 MD15G1019200.v1.1
prunus_persica Prupe.1G371500_v2.0.a1 Prupe.1G371500_v2.0.a1
pyrus_communis pycom08g01700 pycom15g01600
rosa_chinensis RchiOBHm_Chr6g0302041
rosa_laevigata RLG00000011200
rosa_multiflora Rmu_ssc0000160.1_g000001
rosa_roxburghii Rroxscaffold_7G00166150
rosa_rugosa Rorug06G0311300
rosa_samantha Rh6AG424100 Rh6CG437100
rosa_wichuraiana Rw6G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 429
AccBSI CCGCTC 2 cut(s) 78, 514
AccI GTMKAC 2 cut(s) 271, 995
AccII CGCG 4 cut(s) 51, 500, 752, 965
AclWI GGATC 2 cut(s) 248, 261
AcoI YGGCCR 1 cut(s) 200
AcvI CACGTG 2 cut(s) 475, 486
AfaI GTAC 4 cut(s) 146, 803, 877, 1054
AfiI CCNNNNNNNGG 1 cut(s) 839
AflIII ACRYGT 2 cut(s) 472, 648
AgsI TTSAA 4 cut(s) 232, 667, 790, 1036
AjiI CACGTC 1 cut(s) 651
AjnI CCWGG 1 cut(s) 1073
AluBI AGCT 4 cut(s) 261, 690, 858, 1028
AluI AGCT 4 cut(s) 261, 690, 858, 1028
Alw21I GWGCWC 3 cut(s) 485, 692, 860
AlwI GGATC 2 cut(s) 248, 261
Ama87I CYCGRG 2 cut(s) 832, 862
AoxI GGCC 3 cut(s) 168, 200, 531
ApeKI GCWGC 5 cut(s) 98, 363, 571, 708, 968
ArsI GACNNNNNNTTYG 2 cut(s) 535, 567
AspS9I GGNCC 2 cut(s) 435, 978
AsuHPI GGTGA 5 cut(s) 349, 602, 684, 863, 884
AvaI CYCGRG 2 cut(s) 832, 862
AvaII GGWCC 2 cut(s) 435, 978
AxyI CCTNAGG 1 cut(s) 425
BamHI GGATCC 1 cut(s) 253
BanI GGYRCC 1 cut(s) 429
BanII GRGCYC 2 cut(s) 692, 860
BbrPI CACGTG 2 cut(s) 475, 486
Bbv12I GWGCWC 3 cut(s) 485, 692, 860
BbvI GCAGC 5 cut(s) 110, 375, 583, 695, 955
BccI CCATC 2 cut(s) 222, 812
BceAI ACGGC 3 cut(s) 187, 310, 777
BciT130I CCWGG 1 cut(s) 1075
BfaI CTAG 2 cut(s) 258, 879
BglII AGATCT 1 cut(s) 174
Bme1390I CCNGG 1 cut(s) 1075
Bme18I GGWCC 2 cut(s) 435, 978
BmeT110I CYCGRG 2 cut(s) 832, 862
BmgBI CACGTC 1 cut(s) 651
BmgT120I GGNCC 2 cut(s) 435, 978
BmiI GGNNCC 5 cut(s) 255, 398, 431, 492, 979
BmrFI CCNGG 1 cut(s) 1075
BplI GAGNNNNNCTC 2 cut(s) 845, 877
BpmI CTGGAG 2 cut(s) 135, 861
Bpu10I CCTNAGC 1 cut(s) 19
BsaAI YACGTR 2 cut(s) 475, 486
BsaJI CCNNGG 4 cut(s) 49, 451, 861, 1073
BsaXI ACNNNNNCTCC 2 cut(s) 768, 798
Bsc4I CCNNNNNNNGG 1 cut(s) 839
Bse118I RCCGGY 2 cut(s) 694, 971
Bse21I CCTNAGG 1 cut(s) 425
Bse3DI GCAATG 1 cut(s) 732
BseBI CCWGG 1 cut(s) 1075
BseDI CCNNGG 4 cut(s) 49, 451, 861, 1073
BseGI GGATG 4 cut(s) 214, 313, 823, 925
BseLI CCNNNNNNNGG 1 cut(s) 839
BseMI GCAATG 1 cut(s) 732
BseXI GCAGC 5 cut(s) 110, 375, 583, 695, 955
BseYI CCCAGC 1 cut(s) 1024
Bsh1236I CGCG 4 cut(s) 51, 500, 752, 965
BshFI GGCC 3 cut(s) 170, 202, 533
BshNI GGYRCC 1 cut(s) 429
BsiHKAI GWGCWC 3 cut(s) 485, 692, 860
BsiHKCI CYCGRG 2 cut(s) 832, 862
BsiSI CCGG 3 cut(s) 433, 695, 972
BslFI GGGAC 2 cut(s) 807, 991
BslI CCNNNNNNNGG 1 cut(s) 839
BsmFI GGGAC 2 cut(s) 807, 991
BsnI GGCC 3 cut(s) 170, 202, 533
BsoBI CYCGRG 2 cut(s) 832, 862
Bsp1286I GDGCHC 3 cut(s) 485, 692, 860
Bsp1407I TGTACA 1 cut(s) 144
Bsp143I GATC 7 cut(s) 31, 149, 174, 253, 310, 805, 886
BspANI GGCC 3 cut(s) 170, 202, 533
BspFNI CGCG 4 cut(s) 51, 500, 752, 965
BspLI GGNNCC 5 cut(s) 255, 398, 431, 492, 979
BspPI GGATC 2 cut(s) 248, 261
BspQI GCTCTTC 1 cut(s) 473
BspT107I GGYRCC 1 cut(s) 429
BsrBI CCGCTC 2 cut(s) 78, 514
BsrDI GCAATG 1 cut(s) 732
BsrFI RCCGGY 2 cut(s) 694, 971
BsrGI TGTACA 1 cut(s) 144
BssAI RCCGGY 2 cut(s) 694, 971
BssECI CCNNGG 4 cut(s) 49, 451, 861, 1073
BssMI GATC 7 cut(s) 31, 149, 174, 253, 310, 805, 886
Bst2UI CCWGG 1 cut(s) 1075
Bst4CI ACNGT 6 cut(s) 269, 337, 383, 416, 582, 961
Bst6I CTCTTC 3 cut(s) 76, 473, 1025
BstAUI TGTACA 1 cut(s) 144
BstBAI YACGTR 2 cut(s) 475, 486
BstC8I GCNNGC 5 cut(s) 7, 161, 168, 628, 973
BstDEI CTNAG 2 cut(s) 19, 425
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 4 cut(s) 214, 313, 823, 925
BstFNI CGCG 4 cut(s) 51, 500, 752, 965
BstKTI GATC 7 cut(s) 34, 152, 177, 256, 313, 808, 889
BstMBI GATC 7 cut(s) 31, 149, 174, 253, 310, 805, 886
BstMWI GCNNNNNNNGC 9 cut(s) 15, 104, 107, 369, 372, 562, 571, 864, 1046
BstNI CCWGG 1 cut(s) 1075
BstNSI RCATGY 1 cut(s) 785
BstSCI CCNGG 1 cut(s) 1073
BstUI CGCG 4 cut(s) 51, 500, 752, 965
BstV1I GCAGC 5 cut(s) 110, 375, 583, 695, 955
BstX2I RGATCY 2 cut(s) 174, 253
BstYI RGATCY 2 cut(s) 174, 253
Bsu36I CCTNAGG 1 cut(s) 425
BsuRI GGCC 3 cut(s) 170, 202, 533
BtgI CCRYGG 1 cut(s) 49
BtrI CACGTC 1 cut(s) 651
BtsCI GGATG 4 cut(s) 214, 313, 823, 925
BtsIMutI CAGTG 1 cut(s) 467
Cac8I GCNNGC 5 cut(s) 7, 161, 168, 628, 973
Cfr10I RCCGGY 2 cut(s) 694, 971
Cfr13I GGNCC 2 cut(s) 435, 978
Cfr42I CCGCGG 1 cut(s) 52
Csp6I GTAC 4 cut(s) 145, 802, 876, 1053
CviAII CATG 4 cut(s) 376, 782, 814, 1045
CviQI GTAC 4 cut(s) 145, 802, 876, 1053
DdeI CTNAG 2 cut(s) 19, 425
DpnI GATC 7 cut(s) 33, 151, 176, 255, 312, 807, 888
DpnII GATC 7 cut(s) 31, 149, 174, 253, 310, 805, 886
EaeI YGGCCR 1 cut(s) 200
Eam1104I CTCTTC 3 cut(s) 76, 473, 1025
EarI CTCTTC 3 cut(s) 76, 473, 1025
EciI GGCGGA 3 cut(s) 320, 666, 1056
Ecl136II GAGCTC 2 cut(s) 690, 858
Eco24I GRGCYC 2 cut(s) 692, 860
Eco47I GGWCC 2 cut(s) 435, 978
Eco53kI GAGCTC 2 cut(s) 690, 858
Eco72I CACGTG 2 cut(s) 475, 486
Eco81I CCTNAGG 1 cut(s) 425
Eco88I CYCGRG 2 cut(s) 832, 862
EcoICRI GAGCTC 2 cut(s) 690, 858
EcoO109I RGGNCCY 1 cut(s) 978
EcoRII CCWGG 1 cut(s) 1073
EcoT38I GRGCYC 2 cut(s) 692, 860
FaeI CATG 4 cut(s) 379, 785, 817, 1048
FaiI YATR 6 cut(s) 199, 377, 783, 815, 948, 1046
FaqI GGGAC 2 cut(s) 807, 991
FatI CATG 4 cut(s) 375, 781, 813, 1044
FauI CCCGC 3 cut(s) 386, 502, 968
FblI GTMKAC 2 cut(s) 271, 995
FokI GGATG 4 cut(s) 201, 320, 830, 912
FriOI GRGCYC 2 cut(s) 692, 860
FspBI CTAG 2 cut(s) 258, 879
GsaI CCCAGC 1 cut(s) 1028
GsuI CTGGAG 2 cut(s) 135, 861
HaeIII GGCC 3 cut(s) 170, 202, 533
HapII CCGG 3 cut(s) 433, 695, 972
Hin1II CATG 4 cut(s) 379, 785, 817, 1048
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 2 cut(s) 70, 667
HpaII CCGG 3 cut(s) 433, 695, 972
HphI GGTGA 5 cut(s) 349, 602, 684, 863, 884
Hpy166II GTNNAC 3 cut(s) 272, 769, 996
Hpy188I TCNGA 4 cut(s) 42, 75, 646, 776
Hpy188III TCNNGA 2 cut(s) 386, 840
Hpy8I GTNNAC 3 cut(s) 272, 769, 996
Hpy99I CGWCG 2 cut(s) 296, 510
HpyAV CCTTC 2 cut(s) 651, 1023
HpyCH4III ACNGT 6 cut(s) 269, 337, 383, 416, 582, 961
HpyCH4IV ACGT 4 cut(s) 474, 485, 650, 1001
HpyCH4V TGCA 2 cut(s) 444, 725
HpyF10VI GCNNNNNNNGC 9 cut(s) 15, 104, 107, 369, 372, 562, 571, 864, 1046
HpyF3I CTNAG 2 cut(s) 19, 425
HpySE526I ACGT 4 cut(s) 474, 485, 650, 1001
Hsp92II CATG 4 cut(s) 379, 785, 817, 1048
KroI GCCGGC 1 cut(s) 971
KroNI GCCGGC 1 cut(s) 973
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 7 cut(s) 31, 149, 174, 253, 310, 805, 886
LguI GCTCTTC 1 cut(s) 473
LmnI GCTCC 3 cut(s) 511, 863, 1033
Lsp1109I GCAGC 5 cut(s) 110, 375, 583, 695, 955
MaeI CTAG 2 cut(s) 258, 879
MaeII ACGT 4 cut(s) 474, 485, 650, 1001
MaeIII GTNAC 2 cut(s) 204, 955
MalI GATC 7 cut(s) 33, 151, 176, 255, 312, 807, 888
MbiI CCGCTC 2 cut(s) 78, 514
MboI GATC 7 cut(s) 31, 149, 174, 253, 310, 805, 886
MboII GAAGA 4 cut(s) 93, 418, 490, 1012
MflI RGATCY 2 cut(s) 174, 253
MhlI GDGCHC 3 cut(s) 485, 692, 860
MluCI AATT 2 cut(s) 524, 746
MmeI TCCRAC 2 cut(s) 639, 669
MroNI GCCGGC 1 cut(s) 971
MseI TTAA 1 cut(s) 1081
MspA1I CMGCKG 4 cut(s) 51, 101, 366, 708
MspI CCGG 3 cut(s) 433, 695, 972
MspR9I CCNGG 1 cut(s) 1075
MvaI CCWGG 1 cut(s) 1075
MvnI CGCG 4 cut(s) 51, 500, 752, 965
MwoI GCNNNNNNNGC 9 cut(s) 15, 104, 107, 369, 372, 562, 571, 864, 1046
NaeI GCCGGC 1 cut(s) 973
NdeII GATC 7 cut(s) 31, 149, 174, 253, 310, 805, 886
NgoMIV GCCGGC 1 cut(s) 971
NlaIII CATG 4 cut(s) 379, 785, 817, 1048
NlaIV GGNNCC 5 cut(s) 255, 398, 431, 492, 979
NmeAIII GCCGAG 3 cut(s) 196, 476, 509
NmuCI GTSAC 1 cut(s) 204
NspI RCATGY 1 cut(s) 785
PciSI GCTCTTC 1 cut(s) 473
PdiI GCCGGC 1 cut(s) 973
PfeI GAWTC 2 cut(s) 70, 667
PmaCI CACGTG 2 cut(s) 475, 486
PmlI CACGTG 2 cut(s) 475, 486
Ppu21I YACGTR 2 cut(s) 475, 486
PpuMI RGGWCCY 1 cut(s) 978
Psp124BI GAGCTC 2 cut(s) 692, 860
Psp5II RGGWCCY 1 cut(s) 978
Psp6I CCWGG 1 cut(s) 1073
PspCI CACGTG 2 cut(s) 475, 486
PspFI CCCAGC 1 cut(s) 1024
PspGI CCWGG 1 cut(s) 1073
PspN4I GGNNCC 5 cut(s) 255, 398, 431, 492, 979
PspPI GGNCC 2 cut(s) 435, 978
PspPPI RGGWCCY 1 cut(s) 978
PsuI RGATCY 2 cut(s) 174, 253
RsaI GTAC 4 cut(s) 146, 803, 877, 1054
RsaNI GTAC 4 cut(s) 145, 802, 876, 1053
SacI GAGCTC 2 cut(s) 692, 860
SacII CCGCGG 1 cut(s) 52
SapI GCTCTTC 1 cut(s) 473
SaqAI TTAA 1 cut(s) 1081
Sau3AI GATC 7 cut(s) 31, 149, 174, 253, 310, 805, 886
Sau96I GGNCC 2 cut(s) 435, 978
ScrFI CCNGG 1 cut(s) 1075
SduI GDGCHC 3 cut(s) 485, 692, 860
Sfr303I CCGCGG 1 cut(s) 52
SgrAI CRCCGGYG 1 cut(s) 694
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 2 cut(s) 435, 978
Sse9I AATT 2 cut(s) 524, 746
SspMI CTAG 2 cut(s) 258, 879
SstI GAGCTC 2 cut(s) 692, 860
StyD4I CCNGG 1 cut(s) 1073
TaaI ACNGT 6 cut(s) 269, 337, 383, 416, 582, 961
TaiI ACGT 4 cut(s) 477, 488, 653, 1004
TaqI TCGA 1 cut(s) 586
TasI AATT 2 cut(s) 524, 746
TatI WGTACW 1 cut(s) 144
TfiI GAWTC 2 cut(s) 70, 667
Tru1I TTAA 1 cut(s) 1081
Tru9I TTAA 1 cut(s) 1081
TscAI CASTG 1 cut(s) 467
TseFI GTSAC 1 cut(s) 204
TseI GCWGC 5 cut(s) 98, 363, 571, 708, 968
Tsp45I GTSAC 1 cut(s) 204
TspDTI ATGAA 2 cut(s) 392, 1033
TspGWI ACGGA 2 cut(s) 915, 981
TspRI CASTG 1 cut(s) 467
VpaK11BI GGWCC 2 cut(s) 435, 978
XceI RCATGY 1 cut(s) 785
XmiI GTMKAC 2 cut(s) 271, 995
XspI CTAG 2 cut(s) 258, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.