Rroxscaffold_7G00166150

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0012802
Physical Location & Seq
Reverse (-)
7711528 .. 7713731
2204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00166150.1

Sequence Viewer

Length: 726 bp
ATGCGACAAGGAAGCGGCGGTGGCGGTAGTGGTTTAGGATCGGGAGGAATGAATTGTCAGGATTGCGGGAATCAAGCTAAGAAAGACTGTCCTCACTTGCGGTGCCGAACCTGTTGCAAGAGCCGAGGGTTTCAGTGCCAAACACACGTCAAGAGCACTTGGGTTCCTGCCGCGAAACGCCGCGAGAGGCAGGAACAATTCTCTGCTCTTCAGCAACAACAACAACAACAGCAGCAGTTACAGTTTCCGAAAAGGCAGAGAGACAATCAAGCAGGAGGAGCTGGAGGCTCTTCTCTAGCCTGCGTTCGTATACCCACTAACACGTCAGGGTTGGAGCTTGCACCATTTCCAGCGGAGGTGAGCTCGCCAGCCCTGTTCCGGTGTGTGAGAGTCAGTGCAATGGACGATGTGGAGGAGCAGTACGCGTACCAGACGGCGGTGAACATCGGAGGGCACGTGTTCAAGGGACTTCTCTACGATCAAGGACTGGATGGTCATTACACAAGCGCAACCACCGCCGGCGATAGCTCCTCAGGCCAACAAGACGGAGGAGGCGGAGGGGGACATCATCATAATAATCAACAGCAACTTAATCTTATAACCACCACCACAAGCACAAGTGCCAACCCACCTACGGCGACGTTGCTCGACCCTACGGTGGTGTTTCCGACTCCGCTGAATGCTTTTGTGGCTGGTACGCAATACTTCCCACCTCCGAGGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

241

Amino Acids

25.64

Weight (kDa)

8.6

Isoelectric Point (pI)

59.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 16 - 164 1.3e-64 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19790 AT1G19790 AT1G75520
fragaria_vesca FvH4_2g38950
malus_domestica MD08G1021500.v1.1 MD15G1019200.v1.1
prunus_persica Prupe.1G371500_v2.0.a1 Prupe.1G371500_v2.0.a1
pyrus_communis pycom08g01700 pycom15g01600
rosa_chinensis RchiOBHm_Chr6g0302041
rosa_laevigata RLG00000011200
rosa_multiflora Rmu_ssc0000160.1_g000001
rosa_roxburghii Rroxscaffold_7G00166150
rosa_rugosa Rorug06G0311300
rosa_samantha Rh6AG424100 Rh6CG437100
rosa_wichuraiana Rw6G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 599
AasI GACNNNNNNGTC 1 cut(s) 492
AccB1I GGYRCC 1 cut(s) 102
AccI GTMKAC 1 cut(s) 310
AccII CGCG 3 cut(s) 173, 183, 425
AclWI GGATC 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 194
AcvI CACGTG 1 cut(s) 457
AfaI GTAC 3 cut(s) 422, 428, 697
AfiI CCNNNNNNNGG 4 cut(s) 378, 436, 634, 658
AflIII ACRYGT 4 cut(s) 145, 321, 423, 456
AgsI TTSAA 1 cut(s) 463
AjiI CACGTC 2 cut(s) 148, 324
AluBI AGCT 5 cut(s) 77, 281, 337, 363, 528
AluI AGCT 5 cut(s) 77, 281, 337, 363, 528
Alw21I GWGCWC 2 cut(s) 158, 365
Alw26I GTCTC 1 cut(s) 255
AlwI GGATC 1 cut(s) 46
AoxI GGCC 1 cut(s) 535
ApeKI GCWGC 1 cut(s) 232
AspLEI GCGC 1 cut(s) 509
AsuHPI GGTGA 2 cut(s) 370, 451
AxyI CCTNAGG 1 cut(s) 532
BaeGI GKGCMC 1 cut(s) 456
BanI GGYRCC 1 cut(s) 102
BanII GRGCYC 1 cut(s) 365
BbrPI CACGTG 1 cut(s) 457
Bbv12I GWGCWC 2 cut(s) 158, 365
BbvI GCAGC 1 cut(s) 244
BccI CCATC 1 cut(s) 485
BceAI ACGGC 2 cut(s) 450, 651
BcgI CGANNNNNNTGC 2 cut(s) 96, 130
BcoDI GTCTC 1 cut(s) 255
BfaI CTAG 1 cut(s) 296
BisI GCNGC 4 cut(s) 16, 171, 181, 233
BlsI GCNGC 4 cut(s) 17, 172, 182, 234
BmgBI CACGTC 2 cut(s) 148, 324
BmiI GGNNCC 2 cut(s) 104, 165
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 457
BsaJI CCNNGG 2 cut(s) 124, 716
BsaWI WCCGGW 1 cut(s) 378
BsaXI ACNNNNNCTCC 4 cut(s) 404, 434, 441, 471
Bsc4I CCNNNNNNNGG 4 cut(s) 378, 436, 634, 658
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 1 cut(s) 492
Bse21I CCTNAGG 1 cut(s) 532
Bse3DI GCAATG 1 cut(s) 405
BseDI CCNNGG 2 cut(s) 124, 716
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 4 cut(s) 378, 436, 634, 658
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 1 cut(s) 492
BseRI GAGGAG 4 cut(s) 291, 428, 520, 564
BseSI GKGCMC 1 cut(s) 456
BseXI GCAGC 1 cut(s) 244
Bsh1236I CGCG 3 cut(s) 173, 183, 425
BshFI GGCC 1 cut(s) 537
BshNI GGYRCC 1 cut(s) 102
BsiHKAI GWGCWC 2 cut(s) 158, 365
BsiSI CCGG 2 cut(s) 379, 519
BslFI GGGAC 2 cut(s) 480, 576
BslI CCNNNNNNNGG 4 cut(s) 378, 436, 634, 658
BsmAI GTCTC 1 cut(s) 255
BsmFI GGGAC 2 cut(s) 480, 576
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 1 cut(s) 537
Bsp1286I GDGCHC 3 cut(s) 158, 365, 456
Bsp143I GATC 2 cut(s) 38, 478
BspANI GGCC 1 cut(s) 537
BspCNI CTCAG 1 cut(s) 545
BspFNI CGCG 3 cut(s) 173, 183, 425
BspLI GGNNCC 2 cut(s) 104, 165
BspPI GGATC 1 cut(s) 46
BspQI GCTCTTC 2 cut(s) 213, 295
BspT107I GGYRCC 1 cut(s) 102
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 518
BsrI ACTGG 1 cut(s) 492
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 124, 716
BssMI GATC 2 cut(s) 38, 478
BssNAI GTATAC 1 cut(s) 311
Bst1107I GTATAC 1 cut(s) 311
Bst4CI ACNGT 3 cut(s) 89, 243, 658
Bst6I CTCTTC 2 cut(s) 213, 295
BstBAI YACGTR 1 cut(s) 457
BstC8I GCNNGC 5 cut(s) 301, 339, 365, 369, 520
BstDEI CTNAG 3 cut(s) 78, 532, 723
BstF5I GGATG 1 cut(s) 496
BstFNI CGCG 3 cut(s) 173, 183, 425
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 2 cut(s) 41, 481
BstMAI GTCTC 1 cut(s) 255
BstMBI GATC 2 cut(s) 38, 478
BstMWI GCNNNNNNNGC 5 cut(s) 21, 278, 515, 534, 689
BstSLI GKGCMC 1 cut(s) 456
BstUI CGCG 3 cut(s) 173, 183, 425
BstV1I GCAGC 1 cut(s) 244
BstZ17I GTATAC 1 cut(s) 311
Bsu36I CCTNAGG 1 cut(s) 532
BsuRI GGCC 1 cut(s) 537
BtrI CACGTC 2 cut(s) 148, 324
BtsCI GGATG 1 cut(s) 496
BtsIMutI CAGTG 2 cut(s) 140, 400
Cac8I GCNNGC 5 cut(s) 301, 339, 365, 369, 520
CfoI GCGC 1 cut(s) 509
Cfr10I RCCGGY 1 cut(s) 518
Csp6I GTAC 3 cut(s) 421, 427, 696
CviQI GTAC 3 cut(s) 421, 427, 696
DdeI CTNAG 3 cut(s) 78, 532, 723
DpnI GATC 2 cut(s) 40, 480
DpnII GATC 2 cut(s) 38, 478
DrdI GACNNNNNNGTC 1 cut(s) 492
DseDI GACNNNNNNGTC 1 cut(s) 492
Eam1104I CTCTTC 2 cut(s) 213, 295
EarI CTCTTC 2 cut(s) 213, 295
EciI GGCGGA 1 cut(s) 570
Ecl136II GAGCTC 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 365
Eco53kI GAGCTC 1 cut(s) 363
Eco57I CTGAAG 1 cut(s) 194
Eco72I CACGTG 1 cut(s) 457
Eco81I CCTNAGG 1 cut(s) 532
EcoICRI GAGCTC 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 365
FaiI YATR 3 cut(s) 311, 573, 599
FaqI GGGAC 2 cut(s) 480, 576
FauI CCCGC 1 cut(s) 59
FblI GTMKAC 1 cut(s) 310
Fnu4HI GCNGC 4 cut(s) 16, 171, 181, 233
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 4 cut(s) 16, 171, 181, 233
FspBI CTAG 1 cut(s) 296
GlaI GCGC 1 cut(s) 508
GluI GCNGC 4 cut(s) 16, 171, 181, 233
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 1 cut(s) 537
HapII CCGG 2 cut(s) 379, 519
HhaI GCGC 1 cut(s) 509
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HinfI GANTC 3 cut(s) 70, 390, 670
HpaII CCGG 2 cut(s) 379, 519
HphI GGTGA 2 cut(s) 370, 451
Hpy166II GTNNAC 2 cut(s) 311, 442
Hpy188I TCNGA 4 cut(s) 249, 449, 669, 717
Hpy188III TCNNGA 3 cut(s) 42, 59, 151
Hpy8I GTNNAC 2 cut(s) 311, 442
Hpy99I CGWCG 1 cut(s) 643
HpyCH4III ACNGT 3 cut(s) 89, 243, 658
HpyCH4IV ACGT 4 cut(s) 147, 323, 456, 641
HpyCH4V TGCA 3 cut(s) 117, 341, 398
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 278, 515, 534, 689
HpyF3I CTNAG 3 cut(s) 78, 532, 723
HpySE526I ACGT 4 cut(s) 147, 323, 456, 641
HspAI GCGC 1 cut(s) 507
KroI GCCGGC 1 cut(s) 518
KroNI GCCGGC 1 cut(s) 520
Kzo9I GATC 2 cut(s) 38, 478
LguI GCTCTTC 2 cut(s) 213, 295
LmnI GCTCC 4 cut(s) 278, 334, 415, 533
Lsp1109I GCAGC 1 cut(s) 244
MaeI CTAG 1 cut(s) 296
MaeII ACGT 4 cut(s) 147, 323, 456, 641
MaeIII GTNAC 1 cut(s) 237
MalI GATC 2 cut(s) 40, 480
MboI GATC 2 cut(s) 38, 478
MboII GAAGA 2 cut(s) 200, 282
MhlI GDGCHC 3 cut(s) 158, 365, 456
MluCI AATT 2 cut(s) 52, 197
MluI ACGCGT 1 cut(s) 423
MlyI GAGTC 2 cut(s) 399, 664
MmeI TCCRAC 2 cut(s) 312, 692
MreI CGCCGGCG 1 cut(s) 518
MroNI GCCGGC 1 cut(s) 518
MseI TTAA 1 cut(s) 591
MspA1I CMGCKG 2 cut(s) 353, 676
MspI CCGG 2 cut(s) 379, 519
Mva1269I GAATGC 1 cut(s) 685
MvnI CGCG 3 cut(s) 173, 183, 425
MwoI GCNNNNNNNGC 5 cut(s) 21, 278, 515, 534, 689
NaeI GCCGGC 1 cut(s) 520
NdeII GATC 2 cut(s) 38, 478
NgoMIV GCCGGC 1 cut(s) 518
NlaIV GGNNCC 2 cut(s) 104, 165
NmeAIII GCCGAG 1 cut(s) 149
PciSI GCTCTTC 2 cut(s) 213, 295
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PctI GAATGC 1 cut(s) 685
PdiI GCCGGC 1 cut(s) 520
PfeI GAWTC 1 cut(s) 70
PkrI GCNGC 4 cut(s) 17, 172, 182, 234
PleI GAGTC 2 cut(s) 398, 664
PmaCI CACGTG 1 cut(s) 457
PmlI CACGTG 1 cut(s) 457
PpsI GAGTC 2 cut(s) 398, 664
Ppu21I YACGTR 1 cut(s) 457
PsiI TTATAA 1 cut(s) 599
Psp124BI GAGCTC 1 cut(s) 365
PspCI CACGTG 1 cut(s) 457
PspN4I GGNNCC 2 cut(s) 104, 165
RsaI GTAC 3 cut(s) 422, 428, 697
RsaNI GTAC 3 cut(s) 421, 427, 696
SacI GAGCTC 1 cut(s) 365
SapI GCTCTTC 2 cut(s) 213, 295
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 4 cut(s) 16, 171, 181, 233
Sau3AI GATC 2 cut(s) 38, 478
SchI GAGTC 2 cut(s) 399, 664
SduI GDGCHC 3 cut(s) 158, 365, 456
SgrAI CRCCGGYG 1 cut(s) 518
Sse9I AATT 2 cut(s) 52, 197
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 1 cut(s) 365
TaaI ACNGT 3 cut(s) 89, 243, 658
TaiI ACGT 4 cut(s) 150, 326, 459, 644
TaqI TCGA 1 cut(s) 648
TasI AATT 2 cut(s) 52, 197
TauI GCSGC 3 cut(s) 18, 173, 183
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 2 cut(s) 140, 400
TseI GCWGC 1 cut(s) 232
TspDTI ATGAA 1 cut(s) 65
TspGWI ACGGA 1 cut(s) 561
TspRI CASTG 2 cut(s) 140, 400
XmiI GTMKAC 1 cut(s) 310
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.