MD08G1021500.v1.1

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012802
Physical Location & Seq
Reverse (-)
1551263 .. 1553745
2483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1021500.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGGCAGGCTTGTTTTGCTTAGGAGGGAGAGATCAAGGCCCGACCAGCGGTGACAAACAAGGGGGAGAGGATCAGAGTGGAAGAGTGGGAGGAGCAGCAGCGGAAGCGGCGGCTGTAGGAGGAGGAGGGAACTTGTTTTTGTACAGATCATCAGGCGAGGCGCAGGCTGAGATCTACAACAAGGGGTTTGATATATGGCCGTCACAATACCACCCACACCAAAATTTGAACTACTACTCGTTTGGAGTGGATCCTAGCTACAACCATAGACACCTGGACAACAACAACGACGGTGTTTCGGCGGATGATCTGTCGGCGGGGTTGAGGCTGACGATGATGAGAGGAGGATTAGGAGGGTTGGGAAGCAGCAGCGGCAGCATGAACTGCCAGGACTGTGGGAACCAAGCAAAGAAAGACTGTCCGCACCTTAGGTGCCGGACCTGTTGCAAGAGCCGAGGGTTTCAGTGCCAAACACACGTGAAGAGCACGTGGGTTCCCGCCGCGAAACGACGGGAGCGGCAGGAACAATTCTCTGCCTTACAGCAAAACCAAAACCAGCAACAACAGCAAGAACAGCAGATACAGTTTCGAGGTGAAAACCCCAAAAGGCTGAGAGATAACTCTCTTGCCTGCGTTCGTATTCCTTCCAACACGTCAGGGTTGGAGTTAAATCCATTTCCACCAGAAGTGAGCTCACCGGCGGTGTTCCGCTGCGTAAAAGTAAGTGCAATGGACGATGTAGATGAGCAATTCGCGTATCAAACGGCTATCAACATCGGAGGGCATGTGTTCAAGGGACTTCTGTACGATCAGGGCATCGATGGTCAGTATTACCCGAGTTCTGGAGGAGGAGGAGGTGAGAGCTCCTCGGGCGGTCGAGGTACTAGTCATGATCACCACCAAGACGGAGGAGGAAGCGGAGGAGGAGGAGGTCATCCACATCATCAGCAACATGATCTTGTAACGGTCGCAGCTGCCGGCGGGACCTCTGGCAATCCGTCTACTACGTTGCTTGACCCTTCTCTCTTCCCAGCTCCACTCAATGCTTTCATGGCTGGTACGCAATACTTTCCGCCTCCGAGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

363

Amino Acids

38.49

Weight (kDa)

6.38

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 126 - 274 1.9e-65 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19790 AT1G19790 AT1G75520
fragaria_vesca FvH4_2g38950
malus_domestica MD08G1021500.v1.1 MD15G1019200.v1.1
prunus_persica Prupe.1G371500_v2.0.a1 Prupe.1G371500_v2.0.a1
pyrus_communis pycom08g01700 pycom15g01600
rosa_chinensis RchiOBHm_Chr6g0302041
rosa_laevigata RLG00000011200
rosa_multiflora Rmu_ssc0000160.1_g000001
rosa_roxburghii Rroxscaffold_7G00166150
rosa_rugosa Rorug06G0311300
rosa_samantha Rh6AG424100 Rh6CG437100
rosa_wichuraiana Rw6G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 432
AccBSI CCGCTC 1 cut(s) 517
AccI GTMKAC 1 cut(s) 1001
AccII CGCG 2 cut(s) 503, 755
AclWI GGATC 3 cut(s) 78, 245, 258
AcoI YGGCCR 1 cut(s) 197
AcsI RAATTY 1 cut(s) 223
AcvI CACGTG 2 cut(s) 478, 489
AfaI GTAC 4 cut(s) 143, 806, 883, 1060
AfiI CCNNNNNNNGG 2 cut(s) 47, 842
AflIII ACRYGT 2 cut(s) 475, 651
AgsI TTSAA 2 cut(s) 229, 793
AhlI ACTAGT 1 cut(s) 884
AjiI CACGTC 1 cut(s) 654
AjnI CCWGG 2 cut(s) 273, 387
AluBI AGCT 5 cut(s) 258, 693, 864, 974, 1034
AluI AGCT 5 cut(s) 258, 693, 864, 974, 1034
Alw21I GWGCWC 3 cut(s) 488, 695, 866
AlwI GGATC 3 cut(s) 78, 245, 258
Ama87I CYCGRG 2 cut(s) 835, 868
AoxI GGCC 2 cut(s) 37, 197
ApeKI GCWGC 8 cut(s) 95, 98, 366, 369, 375, 711, 971, 974
ApoI RAATTY 1 cut(s) 223
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 3 cut(s) 38, 438, 984
AsuHPI GGTGA 5 cut(s) 62, 605, 687, 869, 887
AvaI CYCGRG 2 cut(s) 835, 868
AvaII GGWCC 2 cut(s) 438, 984
AxyI CCTNAGG 1 cut(s) 428
BamHI GGATCC 1 cut(s) 250
BanI GGYRCC 1 cut(s) 432
BanII GRGCYC 2 cut(s) 695, 866
BbrPI CACGTG 2 cut(s) 478, 489
Bbv12I GWGCWC 3 cut(s) 488, 695, 866
BbvI GCAGC 8 cut(s) 107, 110, 378, 381, 387, 698, 961, 983
BccI CCATC 1 cut(s) 815
BceAI ACGGC 2 cut(s) 184, 780
BciT130I CCWGG 2 cut(s) 275, 389
BclI TGATCA 1 cut(s) 892
BcuI ACTAGT 1 cut(s) 884
BfaI CTAG 2 cut(s) 255, 885
BfmI CTRYAG 1 cut(s) 114
BglII AGATCT 1 cut(s) 171
Bme1390I CCNGG 2 cut(s) 275, 389
Bme18I GGWCC 2 cut(s) 438, 984
BmeT110I CYCGRG 2 cut(s) 835, 868
BmgBI CACGTC 1 cut(s) 654
BmgT120I GGNCC 3 cut(s) 38, 438, 984
BmiI GGNNCC 5 cut(s) 252, 401, 434, 495, 985
BmrFI CCNGG 2 cut(s) 275, 389
BmsI GCATC 1 cut(s) 825
BplI GAGNNNNNCTC 2 cut(s) 851, 883
BpmI CTGGAG 1 cut(s) 864
Bpu10I CCTNAGC 1 cut(s) 19
Bsa29I ATCGAT 1 cut(s) 819
BsaAI YACGTR 2 cut(s) 478, 489
BsaJI CCNNGG 3 cut(s) 454, 867, 1079
BsaXI ACNNNNNCTCC 2 cut(s) 771, 801
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 842
Bse118I RCCGGY 2 cut(s) 697, 977
Bse21I CCTNAGG 1 cut(s) 428
Bse3DI GCAATG 1 cut(s) 735
BseBI CCWGG 2 cut(s) 275, 389
BseCI ATCGAT 1 cut(s) 819
BseDI CCNNGG 3 cut(s) 454, 867, 1079
BseGI GGATG 2 cut(s) 310, 934
BseLI CCNNNNNNNGG 2 cut(s) 47, 842
BseMI GCAATG 1 cut(s) 735
BseMII CTCAG 2 cut(s) 159, 602
BseXI GCAGC 8 cut(s) 107, 110, 378, 381, 387, 698, 961, 983
BseYI CCCAGC 1 cut(s) 1030
Bsh1236I CGCG 2 cut(s) 503, 755
Bsh1285I CGRYCG 2 cut(s) 877, 969
BshFI GGCC 2 cut(s) 39, 199
BshNI GGYRCC 1 cut(s) 432
BshVI ATCGAT 1 cut(s) 819
BsiEI CGRYCG 2 cut(s) 877, 969
BsiHKAI GWGCWC 3 cut(s) 488, 695, 866
BsiHKCI CYCGRG 2 cut(s) 835, 868
BsiSI CCGG 3 cut(s) 436, 698, 978
BslFI GGGAC 2 cut(s) 810, 997
BslI CCNNNNNNNGG 2 cut(s) 47, 842
BsmFI GGGAC 2 cut(s) 810, 997
BsnI GGCC 2 cut(s) 39, 199
BsoBI CYCGRG 2 cut(s) 835, 868
Bsp1286I GDGCHC 3 cut(s) 488, 695, 866
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 9 cut(s) 31, 70, 146, 171, 250, 307, 808, 892, 955
BspANI GGCC 2 cut(s) 39, 199
BspCNI CTCAG 2 cut(s) 160, 603
BspDI ATCGAT 1 cut(s) 819
BspFNI CGCG 2 cut(s) 503, 755
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 5 cut(s) 252, 401, 434, 495, 985
BspPI GGATC 3 cut(s) 78, 245, 258
BspQI GCTCTTC 1 cut(s) 476
BspT107I GGYRCC 1 cut(s) 432
BsrBI CCGCTC 1 cut(s) 517
BsrDI GCAATG 1 cut(s) 735
BsrFI RCCGGY 2 cut(s) 697, 977
BsrGI TGTACA 1 cut(s) 141
BssAI RCCGGY 2 cut(s) 697, 977
BssECI CCNNGG 3 cut(s) 454, 867, 1079
BssMI GATC 9 cut(s) 31, 70, 146, 171, 250, 307, 808, 892, 955
Bst2UI CCWGG 2 cut(s) 275, 389
Bst4CI ACNGT 5 cut(s) 293, 395, 419, 585, 967
Bst6I CTCTTC 3 cut(s) 76, 476, 1031
BstAPI GCANNNNNTGC 1 cut(s) 384
BstAUI TGTACA 1 cut(s) 141
BstBAI YACGTR 2 cut(s) 478, 489
BstC8I GCNNGC 4 cut(s) 7, 165, 631, 979
BstDEI CTNAG 4 cut(s) 19, 168, 428, 611
BstF5I GGATG 2 cut(s) 310, 934
BstFNI CGCG 2 cut(s) 503, 755
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 9 cut(s) 34, 73, 149, 174, 253, 310, 811, 895, 958
BstMBI GATC 9 cut(s) 31, 70, 146, 171, 250, 307, 808, 892, 955
BstMCI CGRYCG 2 cut(s) 877, 969
BstNI CCWGG 2 cut(s) 275, 389
BstNSI RCATGY 1 cut(s) 788
BstSCI CCNGG 2 cut(s) 273, 387
BstSFI CTRYAG 1 cut(s) 114
BstUI CGCG 2 cut(s) 503, 755
BstV1I GCAGC 8 cut(s) 107, 110, 378, 381, 387, 698, 961, 983
BstX2I RGATCY 2 cut(s) 171, 250
BstXI CCANNNNNNTGG 1 cut(s) 395
BstYI RGATCY 2 cut(s) 171, 250
Bsu15I ATCGAT 1 cut(s) 819
Bsu36I CCTNAGG 1 cut(s) 428
BsuRI GGCC 2 cut(s) 39, 199
BsuTUI ATCGAT 1 cut(s) 819
BtrI CACGTC 1 cut(s) 654
BtsCI GGATG 2 cut(s) 310, 934
BtsIMutI CAGTG 1 cut(s) 470
Cac8I GCNNGC 4 cut(s) 7, 165, 631, 979
CciI TCATGA 1 cut(s) 889
CfoI GCGC 1 cut(s) 163
Cfr10I RCCGGY 2 cut(s) 697, 977
Cfr13I GGNCC 3 cut(s) 38, 438, 984
ClaI ATCGAT 1 cut(s) 819
Csp6I GTAC 4 cut(s) 142, 805, 882, 1059
CviAII CATG 5 cut(s) 379, 785, 890, 953, 1051
CviQI GTAC 4 cut(s) 142, 805, 882, 1059
DdeI CTNAG 4 cut(s) 19, 168, 428, 611
DpnI GATC 9 cut(s) 33, 72, 148, 173, 252, 309, 810, 894, 957
DpnII GATC 9 cut(s) 31, 70, 146, 171, 250, 307, 808, 892, 955
EaeI YGGCCR 1 cut(s) 197
Eam1104I CTCTTC 3 cut(s) 76, 476, 1031
EarI CTCTTC 3 cut(s) 76, 476, 1031
EciI GGCGGA 2 cut(s) 317, 1062
Ecl136II GAGCTC 2 cut(s) 693, 864
Eco24I GRGCYC 2 cut(s) 695, 866
Eco47I GGWCC 2 cut(s) 438, 984
Eco53kI GAGCTC 2 cut(s) 693, 864
Eco72I CACGTG 2 cut(s) 478, 489
Eco81I CCTNAGG 1 cut(s) 428
Eco88I CYCGRG 2 cut(s) 835, 868
EcoICRI GAGCTC 2 cut(s) 693, 864
EcoO109I RGGNCCY 1 cut(s) 984
EcoRII CCWGG 2 cut(s) 273, 387
EcoT38I GRGCYC 2 cut(s) 695, 866
FaeI CATG 5 cut(s) 382, 788, 893, 956, 1054
FaiI YATR 8 cut(s) 194, 196, 267, 380, 786, 891, 954, 1052
FaqI GGGAC 2 cut(s) 810, 997
FatI CATG 5 cut(s) 378, 784, 889, 952, 1050
FauI CCCGC 3 cut(s) 310, 505, 974
FbaI TGATCA 1 cut(s) 892
FblI GTMKAC 1 cut(s) 1001
FokI GGATG 2 cut(s) 317, 921
FriOI GRGCYC 2 cut(s) 695, 866
FspBI CTAG 2 cut(s) 255, 885
GlaI GCGC 1 cut(s) 162
GsaI CCCAGC 1 cut(s) 1034
GsuI CTGGAG 1 cut(s) 864
HaeIII GGCC 2 cut(s) 39, 199
HapII CCGG 3 cut(s) 436, 698, 978
HhaI GCGC 1 cut(s) 163
Hin1II CATG 5 cut(s) 382, 788, 893, 956, 1054
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HpaII CCGG 3 cut(s) 436, 698, 978
HphI GGTGA 5 cut(s) 62, 605, 687, 869, 887
Hpy166II GTNNAC 1 cut(s) 1002
Hpy188I TCNGA 3 cut(s) 75, 779, 1080
Hpy188III TCNNGA 2 cut(s) 843, 890
Hpy8I GTNNAC 1 cut(s) 1002
Hpy99I CGWCG 2 cut(s) 293, 513
HpyAV CCTTC 2 cut(s) 654, 1029
HpyCH4III ACNGT 5 cut(s) 293, 395, 419, 585, 967
HpyCH4IV ACGT 4 cut(s) 477, 488, 653, 1007
HpyCH4V TGCA 2 cut(s) 447, 728
HpyF3I CTNAG 4 cut(s) 19, 168, 428, 611
HpySE526I ACGT 4 cut(s) 477, 488, 653, 1007
Hsp92II CATG 5 cut(s) 382, 788, 893, 956, 1054
HspAI GCGC 1 cut(s) 161
KroI GCCGGC 1 cut(s) 977
KroNI GCCGGC 1 cut(s) 979
Ksp22I TGATCA 1 cut(s) 892
Kzo9I GATC 9 cut(s) 31, 70, 146, 171, 250, 307, 808, 892, 955
LguI GCTCTTC 1 cut(s) 476
LmnI GCTCC 4 cut(s) 92, 514, 869, 1039
Lsp1109I GCAGC 8 cut(s) 107, 110, 378, 381, 387, 698, 961, 983
LweI GCATC 1 cut(s) 825
MaeI CTAG 2 cut(s) 255, 885
MaeII ACGT 4 cut(s) 477, 488, 653, 1007
MaeIII GTNAC 3 cut(s) 50, 201, 961
MalI GATC 9 cut(s) 33, 72, 148, 173, 252, 309, 810, 894, 957
MbiI CCGCTC 1 cut(s) 517
MboI GATC 9 cut(s) 31, 70, 146, 171, 250, 307, 808, 892, 955
MboII GAAGA 3 cut(s) 93, 493, 1018
MflI RGATCY 2 cut(s) 171, 250
MhlI GDGCHC 3 cut(s) 488, 695, 866
MluCI AATT 3 cut(s) 223, 527, 749
MmeI TCCRAC 2 cut(s) 642, 672
MroNI GCCGGC 1 cut(s) 977
MseI TTAA 2 cut(s) 668, 1087
MspA1I CMGCKG 5 cut(s) 48, 101, 372, 711, 974
MspI CCGG 3 cut(s) 436, 698, 978
MspR9I CCNGG 2 cut(s) 275, 389
MvaI CCWGG 2 cut(s) 275, 389
MvnI CGCG 2 cut(s) 503, 755
NaeI GCCGGC 1 cut(s) 979
NdeII GATC 9 cut(s) 31, 70, 146, 171, 250, 307, 808, 892, 955
NgoMIV GCCGGC 1 cut(s) 977
NlaIII CATG 5 cut(s) 382, 788, 893, 956, 1054
NlaIV GGNNCC 5 cut(s) 252, 401, 434, 495, 985
NmeAIII GCCGAG 1 cut(s) 479
NmuCI GTSAC 2 cut(s) 50, 201
NspI RCATGY 1 cut(s) 788
PagI TCATGA 1 cut(s) 889
PciSI GCTCTTC 1 cut(s) 476
PdiI GCCGGC 1 cut(s) 979
PmaCI CACGTG 2 cut(s) 478, 489
PmlI CACGTG 2 cut(s) 478, 489
Ppu21I YACGTR 2 cut(s) 478, 489
PpuMI RGGWCCY 1 cut(s) 984
Psp124BI GAGCTC 2 cut(s) 695, 866
Psp5II RGGWCCY 1 cut(s) 984
Psp6I CCWGG 2 cut(s) 273, 387
PspCI CACGTG 2 cut(s) 478, 489
PspFI CCCAGC 1 cut(s) 1030
PspGI CCWGG 2 cut(s) 273, 387
PspN4I GGNNCC 5 cut(s) 252, 401, 434, 495, 985
PspPI GGNCC 3 cut(s) 38, 438, 984
PspPPI RGGWCCY 1 cut(s) 984
PsrI GAACNNNNNNTAC 2 cut(s) 564, 596
PsuI RGATCY 2 cut(s) 171, 250
PvuII CAGCTG 1 cut(s) 974
RsaI GTAC 4 cut(s) 143, 806, 883, 1060
RsaNI GTAC 4 cut(s) 142, 805, 882, 1059
SacI GAGCTC 2 cut(s) 695, 866
SapI GCTCTTC 1 cut(s) 476
SaqAI TTAA 2 cut(s) 668, 1087
Sau3AI GATC 9 cut(s) 31, 70, 146, 171, 250, 307, 808, 892, 955
Sau96I GGNCC 3 cut(s) 38, 438, 984
ScrFI CCNGG 2 cut(s) 275, 389
SduI GDGCHC 3 cut(s) 488, 695, 866
SfaNI GCATC 1 cut(s) 825
SfcI CTRYAG 1 cut(s) 114
SgrAI CRCCGGYG 1 cut(s) 697
SinI GGWCC 2 cut(s) 438, 984
SpeI ACTAGT 1 cut(s) 884
Sse9I AATT 3 cut(s) 223, 527, 749
SspMI CTAG 2 cut(s) 255, 885
SstI GAGCTC 2 cut(s) 695, 866
StyD4I CCNGG 2 cut(s) 273, 387
TaaI ACNGT 5 cut(s) 293, 395, 419, 585, 967
TaiI ACGT 4 cut(s) 480, 491, 656, 1010
TaqI TCGA 3 cut(s) 589, 819, 877
TasI AATT 3 cut(s) 223, 527, 749
TatI WGTACW 1 cut(s) 141
TauI GCSGC 5 cut(s) 110, 113, 375, 503, 520
Tru1I TTAA 2 cut(s) 668, 1087
Tru9I TTAA 2 cut(s) 668, 1087
TscAI CASTG 1 cut(s) 470
TseFI GTSAC 2 cut(s) 50, 201
TseI GCWGC 8 cut(s) 95, 98, 366, 369, 375, 711, 971, 974
Tsp45I GTSAC 2 cut(s) 50, 201
TspDTI ATGAA 2 cut(s) 395, 1039
TspGWI ACGGA 2 cut(s) 921, 987
TspRI CASTG 1 cut(s) 470
VpaK11BI GGWCC 2 cut(s) 438, 984
XapI RAATTY 1 cut(s) 223
XceI RCATGY 1 cut(s) 788
XmiI GTMKAC 1 cut(s) 1001
XspI CTAG 2 cut(s) 255, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.