Rw6G036730

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012802
Physical Location & Seq
Forward (+)
61152460 .. 61153915
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G036730.1

Sequence Viewer

Length: 1014 bp
ATGGCTGGGTTCTTCGGTAATTTAGGGGGAAGAGATCAAGGGCCGAGAAACAAACAAGACGGAGACGATGAAAGAGGAGGAAATTTAAACCTGTTTTCGTACGGAAACGAAGAGATCTACAACAACAAGGGGGGTTTCGAGTTATGGCCACAGTATCATCCTCACCATCCTCATCATCATCAGCAAAGCTTGAACTACTACGCATTTGGAGTGGATCCTAGCCGTAATAACCACCACAACAGCTTGGAGGGCAACGTCTCGGATGACTCATCTTCGGGGTTGAGACTCACTGTGATGCGACAAGGAAGCGGCGGTGGCGGTGGCGGTAGTGGTCTAGGATCGGGCGGAATGAATTGTCAGGATTGCGGGAATCAAGCTAAGAAAGACTGTCCTCACTTGCGGTGCCGAACATGTTGCAAGAGCCGAGGGTTTCAGTGCCAAACACACGTCAAGAGCACTTGGGTTCCTGCCGCGAAACGCCGCGAGAGGCAGGAACAATTCTCTGCTCTTCAGCAACAACAACAACAACAACAGCAGTTACAGTTTCCGAAAAGGCAGAGAGACAATCAAGCAGGAGGAGCTGGAGGCTCTTCTCTAGCCTGCGTTCGTATACCCACTAACACGTCAGGGTTGGAGCTTGCACCATTTCCAGCGGAGGTGAGCTCGCCAGCCCTGTTCCGGTGTGTGAGAGTCAGTGCAATGGACGATGTGGAGGAGCAGTACGCGTACCAGACGGCAGTAAACATCGGAGGGCACGTGTTCAAGGGACTTCTCTACGATCAAGGACCGGATGGTCATTACACAAGCGCAACCACCGCCGGCGATAGCTCATCAGGCCAACAAGACGGAGGGGGACATCATCATAATCAACAGCAACTTAATCTTATAAACACCACCACAAGCACAAGTGCCAACCCACCTACGGCGACGTTGCTCGACCCTACGGTGGTGTTTCCGACTCCGCTGAATGCTTTTGTGGCTGGTACGCAATACTTCCCACCTCCGAGGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

337

Amino Acids

36.47

Weight (kDa)

6.94

Isoelectric Point (pI)

56.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 116 - 264 1.8e-64 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19790 AT1G19790 AT1G75520
fragaria_vesca FvH4_2g38950
malus_domestica MD08G1021500.v1.1 MD15G1019200.v1.1
prunus_persica Prupe.1G371500_v2.0.a1 Prupe.1G371500_v2.0.a1
pyrus_communis pycom08g01700 pycom15g01600
rosa_chinensis RchiOBHm_Chr6g0302041
rosa_laevigata RLG00000011200
rosa_multiflora Rmu_ssc0000160.1_g000001
rosa_roxburghii Rroxscaffold_7G00166150
rosa_rugosa Rorug06G0311300
rosa_samantha Rh6AG424100 Rh6CG437100
rosa_wichuraiana Rw6G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 887
AasI GACNNNNNNGTC 1 cut(s) 792
AccB1I GGYRCC 1 cut(s) 402
AccI GTMKAC 1 cut(s) 610
AccII CGCG 3 cut(s) 473, 483, 725
AclWI GGATC 3 cut(s) 209, 222, 346
AcoI YGGCCR 1 cut(s) 146
AcsI RAATTY 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 494
AcvI CACGTG 1 cut(s) 757
AfaI GTAC 4 cut(s) 101, 722, 728, 985
AfiI CCNNNNNNNGG 3 cut(s) 678, 922, 946
AflIII ACRYGT 5 cut(s) 410, 445, 621, 723, 756
AgsI TTSAA 2 cut(s) 193, 763
AjiI CACGTC 2 cut(s) 448, 624
AluBI AGCT 7 cut(s) 189, 243, 377, 581, 637, 663, 828
AluI AGCT 7 cut(s) 189, 243, 377, 581, 637, 663, 828
Alw21I GWGCWC 2 cut(s) 458, 665
Alw26I GTCTC 4 cut(s) 57, 262, 277, 555
AlwI GGATC 3 cut(s) 209, 222, 346
AoxI GGCC 3 cut(s) 41, 146, 835
ApoI RAATTY 1 cut(s) 82
AspLEI GCGC 1 cut(s) 809
AspS9I GGNCC 2 cut(s) 41, 785
AsuHPI GGTGA 2 cut(s) 155, 670
AvaII GGWCC 1 cut(s) 785
BaeGI GKGCMC 1 cut(s) 756
BalI TGGCCA 1 cut(s) 148
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 402
BanII GRGCYC 1 cut(s) 665
BbrPI CACGTG 1 cut(s) 757
Bbv12I GWGCWC 2 cut(s) 458, 665
BccI CCATC 2 cut(s) 174, 785
BceAI ACGGC 3 cut(s) 207, 750, 939
BcgI CGANNNNNNTGC 2 cut(s) 396, 430
BcoDI GTCTC 4 cut(s) 57, 262, 277, 555
BfaI CTAG 3 cut(s) 219, 335, 596
BglII AGATCT 1 cut(s) 114
BisI GCNGC 3 cut(s) 310, 471, 481
BlsI GCNGC 3 cut(s) 311, 472, 482
Bme18I GGWCC 1 cut(s) 785
BmgBI CACGTC 2 cut(s) 448, 624
BmgT120I GGNCC 2 cut(s) 41, 785
BmiI GGNNCC 3 cut(s) 216, 404, 465
BmsI GCATC 1 cut(s) 285
BplI GAGNNNNNCTC 2 cut(s) 647, 679
BpmI CTGGAG 1 cut(s) 603
BsaAI YACGTR 1 cut(s) 757
BsaJI CCNNGG 2 cut(s) 424, 1004
BsaWI WCCGGW 2 cut(s) 678, 787
BsaXI ACNNNNNCTCC 4 cut(s) 704, 734, 741, 771
Bsc4I CCNNNNNNNGG 3 cut(s) 678, 922, 946
Bse118I RCCGGY 1 cut(s) 818
Bse3DI GCAATG 1 cut(s) 705
BseDI CCNNGG 2 cut(s) 424, 1004
BseGI GGATG 4 cut(s) 157, 166, 268, 796
BseLI CCNNNNNNNGG 3 cut(s) 678, 922, 946
BseMI GCAATG 1 cut(s) 705
BseRI GAGGAG 3 cut(s) 90, 591, 728
BseSI GKGCMC 1 cut(s) 756
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 3 cut(s) 473, 483, 725
BshFI GGCC 3 cut(s) 43, 148, 837
BshNI GGYRCC 1 cut(s) 402
BsiHKAI GWGCWC 2 cut(s) 458, 665
BsiSI CCGG 3 cut(s) 679, 788, 819
BsiWI CGTACG 1 cut(s) 99
BslFI GGGAC 2 cut(s) 780, 867
BslI CCNNNNNNNGG 3 cut(s) 678, 922, 946
BsmAI GTCTC 4 cut(s) 57, 262, 277, 555
BsmBI CGTCTC 2 cut(s) 57, 262
BsmFI GGGAC 2 cut(s) 780, 867
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 3 cut(s) 43, 148, 837
Bsp1286I GDGCHC 3 cut(s) 458, 665, 756
Bsp143I GATC 5 cut(s) 34, 114, 214, 338, 778
BspANI GGCC 3 cut(s) 43, 148, 837
BspFNI CGCG 3 cut(s) 473, 483, 725
BspLI GGNNCC 3 cut(s) 216, 404, 465
BspPI GGATC 3 cut(s) 209, 222, 346
BspQI GCTCTTC 2 cut(s) 513, 595
BspT107I GGYRCC 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 705
BsrFI RCCGGY 1 cut(s) 818
BssAI RCCGGY 1 cut(s) 818
BssECI CCNNGG 2 cut(s) 424, 1004
BssMI GATC 5 cut(s) 34, 114, 214, 338, 778
BssNAI GTATAC 1 cut(s) 611
Bst1107I GTATAC 1 cut(s) 611
Bst4CI ACNGT 5 cut(s) 153, 292, 389, 543, 946
Bst6I CTCTTC 4 cut(s) 25, 105, 513, 595
BstBAI YACGTR 1 cut(s) 757
BstC8I GCNNGC 5 cut(s) 601, 639, 665, 669, 820
BstDEI CTNAG 2 cut(s) 378, 1011
BstF5I GGATG 4 cut(s) 157, 166, 268, 796
BstFNI CGCG 3 cut(s) 473, 483, 725
BstHHI GCGC 1 cut(s) 809
BstKTI GATC 5 cut(s) 37, 117, 217, 341, 781
BstMAI GTCTC 4 cut(s) 57, 262, 277, 555
BstMBI GATC 5 cut(s) 34, 114, 214, 338, 778
BstMWI GCNNNNNNNGC 6 cut(s) 249, 315, 578, 815, 834, 977
BstNSI RCATGY 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 756
BstUI CGCG 3 cut(s) 473, 483, 725
BstX2I RGATCY 2 cut(s) 114, 214
BstYI RGATCY 2 cut(s) 114, 214
BstZ17I GTATAC 1 cut(s) 611
BsuRI GGCC 3 cut(s) 43, 148, 837
BtrI CACGTC 2 cut(s) 448, 624
BtsCI GGATG 4 cut(s) 157, 166, 268, 796
BtsIMutI CAGTG 3 cut(s) 288, 440, 700
Cac8I GCNNGC 5 cut(s) 601, 639, 665, 669, 820
CfoI GCGC 1 cut(s) 809
Cfr10I RCCGGY 1 cut(s) 818
Cfr13I GGNCC 2 cut(s) 41, 785
Csp6I GTAC 4 cut(s) 100, 721, 727, 984
CviAII CATG 1 cut(s) 411
CviQI GTAC 4 cut(s) 100, 721, 727, 984
DdeI CTNAG 2 cut(s) 378, 1011
DpnI GATC 5 cut(s) 36, 116, 216, 340, 780
DpnII GATC 5 cut(s) 34, 114, 214, 338, 778
DraI TTTAAA 1 cut(s) 87
DrdI GACNNNNNNGTC 1 cut(s) 792
DseDI GACNNNNNNGTC 1 cut(s) 792
EaeI YGGCCR 1 cut(s) 146
Eam1104I CTCTTC 4 cut(s) 25, 105, 513, 595
EarI CTCTTC 4 cut(s) 25, 105, 513, 595
EciI GGCGGA 1 cut(s) 360
Ecl136II GAGCTC 1 cut(s) 663
Eco24I GRGCYC 1 cut(s) 665
Eco47I GGWCC 1 cut(s) 785
Eco53kI GAGCTC 1 cut(s) 663
Eco57I CTGAAG 1 cut(s) 494
Eco72I CACGTG 1 cut(s) 757
EcoICRI GAGCTC 1 cut(s) 663
EcoT38I GRGCYC 1 cut(s) 665
Esp3I CGTCTC 2 cut(s) 57, 262
FaeI CATG 1 cut(s) 414
FaiI YATR 5 cut(s) 145, 412, 611, 864, 887
FaqI GGGAC 2 cut(s) 780, 867
FatI CATG 1 cut(s) 410
FauI CCCGC 1 cut(s) 359
FblI GTMKAC 1 cut(s) 610
Fnu4HI GCNGC 3 cut(s) 310, 471, 481
FokI GGATG 4 cut(s) 144, 153, 275, 803
FriOI GRGCYC 1 cut(s) 665
Fsp4HI GCNGC 3 cut(s) 310, 471, 481
FspBI CTAG 3 cut(s) 219, 335, 596
GlaI GCGC 1 cut(s) 808
GluI GCNGC 3 cut(s) 310, 471, 481
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 603
HaeIII GGCC 3 cut(s) 43, 148, 837
HapII CCGG 3 cut(s) 679, 788, 819
HhaI GCGC 1 cut(s) 809
Hin1II CATG 1 cut(s) 414
Hin6I GCGC 1 cut(s) 807
HinP1I GCGC 1 cut(s) 807
HindIII AAGCTT 1 cut(s) 187
HinfI GANTC 5 cut(s) 266, 285, 370, 690, 958
HpaII CCGG 3 cut(s) 679, 788, 819
HphI GGTGA 2 cut(s) 155, 670
Hpy166II GTNNAC 2 cut(s) 611, 742
Hpy188I TCNGA 5 cut(s) 262, 549, 749, 957, 1005
Hpy188III TCNNGA 2 cut(s) 359, 451
Hpy8I GTNNAC 2 cut(s) 611, 742
Hpy99I CGWCG 1 cut(s) 931
HpyCH4III ACNGT 5 cut(s) 153, 292, 389, 543, 946
HpyCH4IV ACGT 5 cut(s) 255, 447, 623, 756, 929
HpyCH4V TGCA 3 cut(s) 417, 641, 698
HpyF10VI GCNNNNNNNGC 6 cut(s) 249, 315, 578, 815, 834, 977
HpyF3I CTNAG 2 cut(s) 378, 1011
HpySE526I ACGT 5 cut(s) 255, 447, 623, 756, 929
Hsp92II CATG 1 cut(s) 414
HspAI GCGC 1 cut(s) 807
KroI GCCGGC 1 cut(s) 818
KroNI GCCGGC 1 cut(s) 820
Kzo9I GATC 5 cut(s) 34, 114, 214, 338, 778
LguI GCTCTTC 2 cut(s) 513, 595
LmnI GCTCC 3 cut(s) 578, 634, 715
LweI GCATC 1 cut(s) 285
MaeI CTAG 3 cut(s) 219, 335, 596
MaeII ACGT 5 cut(s) 255, 447, 623, 756, 929
MaeIII GTNAC 1 cut(s) 537
MalI GATC 5 cut(s) 36, 116, 216, 340, 780
MboI GATC 5 cut(s) 34, 114, 214, 338, 778
MboII GAAGA 6 cut(s) 4, 42, 122, 264, 500, 582
MflI RGATCY 2 cut(s) 114, 214
MhlI GDGCHC 3 cut(s) 458, 665, 756
MlsI TGGCCA 1 cut(s) 148
MluCI AATT 4 cut(s) 19, 82, 352, 497
MluI ACGCGT 1 cut(s) 723
MluNI TGGCCA 1 cut(s) 148
MlyI GAGTC 4 cut(s) 260, 279, 699, 952
MmeI TCCRAC 2 cut(s) 612, 980
Mox20I TGGCCA 1 cut(s) 148
MreI CGCCGGCG 1 cut(s) 818
MroNI GCCGGC 1 cut(s) 818
MscI TGGCCA 1 cut(s) 148
MseI TTAA 2 cut(s) 86, 879
MslI CAYNNNNRTG 1 cut(s) 293
Msp20I TGGCCA 1 cut(s) 148
MspA1I CMGCKG 2 cut(s) 653, 964
MspI CCGG 3 cut(s) 679, 788, 819
Mva1269I GAATGC 1 cut(s) 973
MvnI CGCG 3 cut(s) 473, 483, 725
MwoI GCNNNNNNNGC 6 cut(s) 249, 315, 578, 815, 834, 977
NaeI GCCGGC 1 cut(s) 820
NdeII GATC 5 cut(s) 34, 114, 214, 338, 778
NgoMIV GCCGGC 1 cut(s) 818
NlaIII CATG 1 cut(s) 414
NlaIV GGNNCC 3 cut(s) 216, 404, 465
NmeAIII GCCGAG 2 cut(s) 69, 449
NspI RCATGY 1 cut(s) 414
PciI ACATGT 1 cut(s) 410
PciSI GCTCTTC 2 cut(s) 513, 595
PcsI WCGNNNNNNNCGW 1 cut(s) 753
PctI GAATGC 1 cut(s) 973
PdiI GCCGGC 1 cut(s) 820
PfeI GAWTC 1 cut(s) 370
Pfl23II CGTACG 1 cut(s) 99
PkrI GCNGC 3 cut(s) 311, 472, 482
PleI GAGTC 4 cut(s) 260, 279, 698, 952
PmaCI CACGTG 1 cut(s) 757
PmlI CACGTG 1 cut(s) 757
PpsI GAGTC 4 cut(s) 260, 279, 698, 952
Ppu21I YACGTR 1 cut(s) 757
PscI ACATGT 1 cut(s) 410
PsiI TTATAA 1 cut(s) 887
Psp124BI GAGCTC 1 cut(s) 665
PspCI CACGTG 1 cut(s) 757
PspFI CCCAGC 1 cut(s) 5
PspLI CGTACG 1 cut(s) 99
PspN4I GGNNCC 3 cut(s) 216, 404, 465
PspPI GGNCC 2 cut(s) 41, 785
PsuI RGATCY 2 cut(s) 114, 214
RsaI GTAC 4 cut(s) 101, 722, 728, 985
RsaNI GTAC 4 cut(s) 100, 721, 727, 984
RseI CAYNNNNRTG 1 cut(s) 293
SacI GAGCTC 1 cut(s) 665
SapI GCTCTTC 2 cut(s) 513, 595
SaqAI TTAA 2 cut(s) 86, 879
SatI GCNGC 3 cut(s) 310, 471, 481
Sau3AI GATC 5 cut(s) 34, 114, 214, 338, 778
Sau96I GGNCC 2 cut(s) 41, 785
SchI GAGTC 4 cut(s) 260, 279, 699, 952
SduI GDGCHC 3 cut(s) 458, 665, 756
SfaNI GCATC 1 cut(s) 285
SgrAI CRCCGGYG 1 cut(s) 818
SinI GGWCC 1 cut(s) 785
SmiMI CAYNNNNRTG 1 cut(s) 293
Sse9I AATT 4 cut(s) 19, 82, 352, 497
SspMI CTAG 3 cut(s) 219, 335, 596
SstI GAGCTC 1 cut(s) 665
TaaI ACNGT 5 cut(s) 153, 292, 389, 543, 946
TaiI ACGT 5 cut(s) 258, 450, 626, 759, 932
TaqI TCGA 2 cut(s) 138, 936
TasI AATT 4 cut(s) 19, 82, 352, 497
TauI GCSGC 3 cut(s) 312, 473, 483
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 2 cut(s) 86, 879
Tru9I TTAA 2 cut(s) 86, 879
TscAI CASTG 3 cut(s) 295, 440, 700
TspDTI ATGAA 2 cut(s) 84, 365
TspGWI ACGGA 3 cut(s) 75, 117, 861
TspRI CASTG 3 cut(s) 295, 440, 700
VpaK11BI GGWCC 1 cut(s) 785
XapI RAATTY 1 cut(s) 82
XceI RCATGY 1 cut(s) 414
XmiI GTMKAC 1 cut(s) 610
XspI CTAG 3 cut(s) 219, 335, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.