Rh6AG424100

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0012802
Physical Location & Seq
Forward (+)
61651823 .. 61653284
1462 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG424100.1

Sequence Viewer

Length: 1020 bp
ATGGCTGGGTTCTTCGGTAATTTAGGGGGAAGAGATCAAGGGCCGAGAAACAAACAAGACGGAGACGATGAAAGAGGAGGAAATTTAAACCTGTTTTCGTACGGAAACGAAGAGATCTACAACAACAAGGGGGGTTTCGAGTTATGGCCACAGTATCATCCTCACCATCCTCATCATCATCAGCAAAGCTTGAACTACTACGCATTTGGAGTGGATCCTAGCCGTAATAACCACCACAACAGCTTGGAGGGCAACGTCTCGGATGACTCATCTTCGGGGTTGAGACTCACTGTGATGCGACAAGGAAGCGGCGGTGGCGGTGGCGGTGGCGGTAGTGGTCTAGGATCGGGCGGAATGAATTGTCAGGATTGCGGGAATCAAGCTAAGAAAGACTGTCCTCACTTGCGGTGCCGAACATGTTGCAAGAGCCGAGGGTTTCAGTGCCAAACACACGTCAAGAGCACTTGGGTTCCTGCCGCGAAACGCCGCGAGAGGCAGGAACAATTCTCTGCTCTTCAGCAACAACAACAACAACAACAGCAGTTACAGTTTCCGAAAAGGCAGAGAGACAATCAAGCAGGAGGAGCTGGAGGCTCTTCTCTAGCCTGCGTTCGTATACCCACTAACACGTCAGGGTTGGAGCTTGCACCATTTCCAGCGGAGGTGAGCTCGCCAGCCCTGTTCCGGTGTGTGAGAGTCAGTGCAATGGACGATGTGGAGGAGCAGTACGCGTACCAGACGGCAGTAAACATCGGAGGGCACGTGTTCAAGGGACTTCTCTACGATCAAGGACCGGATGGTCATTACACAAGCGCAACCACCGCCGGCGATAGCTCATCAGGCCAACAAGACGGAGGGGGACATCATCATAATCAACAGCAACTTAATCTTATAAACACCACCACAAGCACAAGTGCCAACCCACCTACGGCGACGTTGCTCGACCCTACGGTGGTGTTTCCGACTCCGCTGAATGCTTTTGTGGCTGGTACGCAATACTTCCCACCTCCGAGGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

339

Amino Acids

36.59

Weight (kDa)

6.94

Isoelectric Point (pI)

57.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 118 - 266 1.8e-64 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19790 AT1G19790 AT1G75520
fragaria_vesca FvH4_2g38950
malus_domestica MD08G1021500.v1.1 MD15G1019200.v1.1
prunus_persica Prupe.1G371500_v2.0.a1 Prupe.1G371500_v2.0.a1
pyrus_communis pycom08g01700 pycom15g01600
rosa_chinensis RchiOBHm_Chr6g0302041
rosa_laevigata RLG00000011200
rosa_multiflora Rmu_ssc0000160.1_g000001
rosa_roxburghii Rroxscaffold_7G00166150
rosa_rugosa Rorug06G0311300
rosa_samantha Rh6AG424100 Rh6CG437100
rosa_wichuraiana Rw6G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 893
AasI GACNNNNNNGTC 1 cut(s) 798
AccB1I GGYRCC 1 cut(s) 408
AccI GTMKAC 1 cut(s) 616
AccII CGCG 3 cut(s) 479, 489, 731
AclWI GGATC 3 cut(s) 209, 222, 352
AcoI YGGCCR 1 cut(s) 146
AcsI RAATTY 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 500
AcvI CACGTG 1 cut(s) 763
AfaI GTAC 4 cut(s) 101, 728, 734, 991
AfiI CCNNNNNNNGG 3 cut(s) 684, 928, 952
AflIII ACRYGT 5 cut(s) 416, 451, 627, 729, 762
AgsI TTSAA 2 cut(s) 193, 769
AjiI CACGTC 2 cut(s) 454, 630
AluBI AGCT 7 cut(s) 189, 243, 383, 587, 643, 669, 834
AluI AGCT 7 cut(s) 189, 243, 383, 587, 643, 669, 834
Alw21I GWGCWC 2 cut(s) 464, 671
Alw26I GTCTC 4 cut(s) 57, 262, 277, 561
AlwI GGATC 3 cut(s) 209, 222, 352
AoxI GGCC 3 cut(s) 41, 146, 841
ApoI RAATTY 1 cut(s) 82
AspLEI GCGC 1 cut(s) 815
AspS9I GGNCC 2 cut(s) 41, 791
AsuHPI GGTGA 2 cut(s) 155, 676
AvaII GGWCC 1 cut(s) 791
BaeGI GKGCMC 1 cut(s) 762
BalI TGGCCA 1 cut(s) 148
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 408
BanII GRGCYC 1 cut(s) 671
BbrPI CACGTG 1 cut(s) 763
Bbv12I GWGCWC 2 cut(s) 464, 671
BccI CCATC 2 cut(s) 174, 791
BceAI ACGGC 3 cut(s) 207, 756, 945
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BcoDI GTCTC 4 cut(s) 57, 262, 277, 561
BfaI CTAG 3 cut(s) 219, 341, 602
BglII AGATCT 1 cut(s) 114
BisI GCNGC 3 cut(s) 310, 477, 487
BlsI GCNGC 3 cut(s) 311, 478, 488
Bme18I GGWCC 1 cut(s) 791
BmgBI CACGTC 2 cut(s) 454, 630
BmgT120I GGNCC 2 cut(s) 41, 791
BmiI GGNNCC 3 cut(s) 216, 410, 471
BmsI GCATC 1 cut(s) 285
BplI GAGNNNNNCTC 2 cut(s) 653, 685
BpmI CTGGAG 1 cut(s) 609
BsaAI YACGTR 1 cut(s) 763
BsaJI CCNNGG 2 cut(s) 430, 1010
BsaWI WCCGGW 2 cut(s) 684, 793
BsaXI ACNNNNNCTCC 4 cut(s) 710, 740, 747, 777
Bsc4I CCNNNNNNNGG 3 cut(s) 684, 928, 952
Bse118I RCCGGY 1 cut(s) 824
Bse3DI GCAATG 1 cut(s) 711
BseDI CCNNGG 2 cut(s) 430, 1010
BseGI GGATG 4 cut(s) 157, 166, 268, 802
BseLI CCNNNNNNNGG 3 cut(s) 684, 928, 952
BseMI GCAATG 1 cut(s) 711
BseRI GAGGAG 3 cut(s) 90, 597, 734
BseSI GKGCMC 1 cut(s) 762
BseYI CCCAGC 1 cut(s) 5
Bsh1236I CGCG 3 cut(s) 479, 489, 731
BshFI GGCC 3 cut(s) 43, 148, 843
BshNI GGYRCC 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 464, 671
BsiSI CCGG 3 cut(s) 685, 794, 825
BsiWI CGTACG 1 cut(s) 99
BslFI GGGAC 2 cut(s) 786, 873
BslI CCNNNNNNNGG 3 cut(s) 684, 928, 952
BsmAI GTCTC 4 cut(s) 57, 262, 277, 561
BsmBI CGTCTC 2 cut(s) 57, 262
BsmFI GGGAC 2 cut(s) 786, 873
BsmI GAATGC 1 cut(s) 979
BsnI GGCC 3 cut(s) 43, 148, 843
Bsp1286I GDGCHC 3 cut(s) 464, 671, 762
Bsp143I GATC 5 cut(s) 34, 114, 214, 344, 784
BspANI GGCC 3 cut(s) 43, 148, 843
BspFNI CGCG 3 cut(s) 479, 489, 731
BspLI GGNNCC 3 cut(s) 216, 410, 471
BspPI GGATC 3 cut(s) 209, 222, 352
BspQI GCTCTTC 2 cut(s) 519, 601
BspT107I GGYRCC 1 cut(s) 408
BsrDI GCAATG 1 cut(s) 711
BsrFI RCCGGY 1 cut(s) 824
BssAI RCCGGY 1 cut(s) 824
BssECI CCNNGG 2 cut(s) 430, 1010
BssMI GATC 5 cut(s) 34, 114, 214, 344, 784
BssNAI GTATAC 1 cut(s) 617
Bst1107I GTATAC 1 cut(s) 617
Bst4CI ACNGT 5 cut(s) 153, 292, 395, 549, 952
Bst6I CTCTTC 4 cut(s) 25, 105, 519, 601
BstBAI YACGTR 1 cut(s) 763
BstC8I GCNNGC 5 cut(s) 607, 645, 671, 675, 826
BstDEI CTNAG 2 cut(s) 384, 1017
BstF5I GGATG 4 cut(s) 157, 166, 268, 802
BstFNI CGCG 3 cut(s) 479, 489, 731
BstHHI GCGC 1 cut(s) 815
BstKTI GATC 5 cut(s) 37, 117, 217, 347, 787
BstMAI GTCTC 4 cut(s) 57, 262, 277, 561
BstMBI GATC 5 cut(s) 34, 114, 214, 344, 784
BstMWI GCNNNNNNNGC 6 cut(s) 249, 315, 584, 821, 840, 983
BstNSI RCATGY 1 cut(s) 420
BstSLI GKGCMC 1 cut(s) 762
BstUI CGCG 3 cut(s) 479, 489, 731
BstX2I RGATCY 2 cut(s) 114, 214
BstYI RGATCY 2 cut(s) 114, 214
BstZ17I GTATAC 1 cut(s) 617
BsuRI GGCC 3 cut(s) 43, 148, 843
BtrI CACGTC 2 cut(s) 454, 630
BtsCI GGATG 4 cut(s) 157, 166, 268, 802
BtsIMutI CAGTG 3 cut(s) 288, 446, 706
Cac8I GCNNGC 5 cut(s) 607, 645, 671, 675, 826
CfoI GCGC 1 cut(s) 815
Cfr10I RCCGGY 1 cut(s) 824
Cfr13I GGNCC 2 cut(s) 41, 791
Csp6I GTAC 4 cut(s) 100, 727, 733, 990
CviAII CATG 1 cut(s) 417
CviQI GTAC 4 cut(s) 100, 727, 733, 990
DdeI CTNAG 2 cut(s) 384, 1017
DpnI GATC 5 cut(s) 36, 116, 216, 346, 786
DpnII GATC 5 cut(s) 34, 114, 214, 344, 784
DraI TTTAAA 1 cut(s) 87
DrdI GACNNNNNNGTC 1 cut(s) 798
DseDI GACNNNNNNGTC 1 cut(s) 798
EaeI YGGCCR 1 cut(s) 146
Eam1104I CTCTTC 4 cut(s) 25, 105, 519, 601
EarI CTCTTC 4 cut(s) 25, 105, 519, 601
EciI GGCGGA 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 669
Eco24I GRGCYC 1 cut(s) 671
Eco47I GGWCC 1 cut(s) 791
Eco53kI GAGCTC 1 cut(s) 669
Eco57I CTGAAG 1 cut(s) 500
Eco72I CACGTG 1 cut(s) 763
EcoICRI GAGCTC 1 cut(s) 669
EcoT38I GRGCYC 1 cut(s) 671
Esp3I CGTCTC 2 cut(s) 57, 262
FaeI CATG 1 cut(s) 420
FaiI YATR 5 cut(s) 145, 418, 617, 870, 893
FaqI GGGAC 2 cut(s) 786, 873
FatI CATG 1 cut(s) 416
FauI CCCGC 1 cut(s) 365
FblI GTMKAC 1 cut(s) 616
Fnu4HI GCNGC 3 cut(s) 310, 477, 487
FokI GGATG 4 cut(s) 144, 153, 275, 809
FriOI GRGCYC 1 cut(s) 671
Fsp4HI GCNGC 3 cut(s) 310, 477, 487
FspBI CTAG 3 cut(s) 219, 341, 602
GlaI GCGC 1 cut(s) 814
GluI GCNGC 3 cut(s) 310, 477, 487
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 609
HaeIII GGCC 3 cut(s) 43, 148, 843
HapII CCGG 3 cut(s) 685, 794, 825
HhaI GCGC 1 cut(s) 815
Hin1II CATG 1 cut(s) 420
Hin6I GCGC 1 cut(s) 813
HinP1I GCGC 1 cut(s) 813
HindIII AAGCTT 1 cut(s) 187
HinfI GANTC 5 cut(s) 266, 285, 376, 696, 964
HpaII CCGG 3 cut(s) 685, 794, 825
HphI GGTGA 2 cut(s) 155, 676
Hpy166II GTNNAC 2 cut(s) 617, 748
Hpy188I TCNGA 5 cut(s) 262, 555, 755, 963, 1011
Hpy188III TCNNGA 2 cut(s) 365, 457
Hpy8I GTNNAC 2 cut(s) 617, 748
Hpy99I CGWCG 1 cut(s) 937
HpyCH4III ACNGT 5 cut(s) 153, 292, 395, 549, 952
HpyCH4IV ACGT 5 cut(s) 255, 453, 629, 762, 935
HpyCH4V TGCA 3 cut(s) 423, 647, 704
HpyF10VI GCNNNNNNNGC 6 cut(s) 249, 315, 584, 821, 840, 983
HpyF3I CTNAG 2 cut(s) 384, 1017
HpySE526I ACGT 5 cut(s) 255, 453, 629, 762, 935
Hsp92II CATG 1 cut(s) 420
HspAI GCGC 1 cut(s) 813
KroI GCCGGC 1 cut(s) 824
KroNI GCCGGC 1 cut(s) 826
Kzo9I GATC 5 cut(s) 34, 114, 214, 344, 784
LguI GCTCTTC 2 cut(s) 519, 601
LmnI GCTCC 3 cut(s) 584, 640, 721
LweI GCATC 1 cut(s) 285
MaeI CTAG 3 cut(s) 219, 341, 602
MaeII ACGT 5 cut(s) 255, 453, 629, 762, 935
MaeIII GTNAC 1 cut(s) 543
MalI GATC 5 cut(s) 36, 116, 216, 346, 786
MboI GATC 5 cut(s) 34, 114, 214, 344, 784
MboII GAAGA 6 cut(s) 4, 42, 122, 264, 506, 588
MflI RGATCY 2 cut(s) 114, 214
MhlI GDGCHC 3 cut(s) 464, 671, 762
MlsI TGGCCA 1 cut(s) 148
MluCI AATT 4 cut(s) 19, 82, 358, 503
MluI ACGCGT 1 cut(s) 729
MluNI TGGCCA 1 cut(s) 148
MlyI GAGTC 4 cut(s) 260, 279, 705, 958
MmeI TCCRAC 2 cut(s) 618, 986
Mox20I TGGCCA 1 cut(s) 148
MreI CGCCGGCG 1 cut(s) 824
MroNI GCCGGC 1 cut(s) 824
MscI TGGCCA 1 cut(s) 148
MseI TTAA 2 cut(s) 86, 885
MslI CAYNNNNRTG 1 cut(s) 293
Msp20I TGGCCA 1 cut(s) 148
MspA1I CMGCKG 2 cut(s) 659, 970
MspI CCGG 3 cut(s) 685, 794, 825
Mva1269I GAATGC 1 cut(s) 979
MvnI CGCG 3 cut(s) 479, 489, 731
MwoI GCNNNNNNNGC 6 cut(s) 249, 315, 584, 821, 840, 983
NaeI GCCGGC 1 cut(s) 826
NdeII GATC 5 cut(s) 34, 114, 214, 344, 784
NgoMIV GCCGGC 1 cut(s) 824
NlaIII CATG 1 cut(s) 420
NlaIV GGNNCC 3 cut(s) 216, 410, 471
NmeAIII GCCGAG 2 cut(s) 69, 455
NspI RCATGY 1 cut(s) 420
PciI ACATGT 1 cut(s) 416
PciSI GCTCTTC 2 cut(s) 519, 601
PcsI WCGNNNNNNNCGW 1 cut(s) 759
PctI GAATGC 1 cut(s) 979
PdiI GCCGGC 1 cut(s) 826
PfeI GAWTC 1 cut(s) 376
Pfl23II CGTACG 1 cut(s) 99
PkrI GCNGC 3 cut(s) 311, 478, 488
PleI GAGTC 4 cut(s) 260, 279, 704, 958
PmaCI CACGTG 1 cut(s) 763
PmlI CACGTG 1 cut(s) 763
PpsI GAGTC 4 cut(s) 260, 279, 704, 958
Ppu21I YACGTR 1 cut(s) 763
PscI ACATGT 1 cut(s) 416
PsiI TTATAA 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 671
PspCI CACGTG 1 cut(s) 763
PspFI CCCAGC 1 cut(s) 5
PspLI CGTACG 1 cut(s) 99
PspN4I GGNNCC 3 cut(s) 216, 410, 471
PspPI GGNCC 2 cut(s) 41, 791
PsuI RGATCY 2 cut(s) 114, 214
RsaI GTAC 4 cut(s) 101, 728, 734, 991
RsaNI GTAC 4 cut(s) 100, 727, 733, 990
RseI CAYNNNNRTG 1 cut(s) 293
SacI GAGCTC 1 cut(s) 671
SapI GCTCTTC 2 cut(s) 519, 601
SaqAI TTAA 2 cut(s) 86, 885
SatI GCNGC 3 cut(s) 310, 477, 487
Sau3AI GATC 5 cut(s) 34, 114, 214, 344, 784
Sau96I GGNCC 2 cut(s) 41, 791
SchI GAGTC 4 cut(s) 260, 279, 705, 958
SduI GDGCHC 3 cut(s) 464, 671, 762
SfaNI GCATC 1 cut(s) 285
SgrAI CRCCGGYG 1 cut(s) 824
SinI GGWCC 1 cut(s) 791
SmiMI CAYNNNNRTG 1 cut(s) 293
Sse9I AATT 4 cut(s) 19, 82, 358, 503
SspMI CTAG 3 cut(s) 219, 341, 602
SstI GAGCTC 1 cut(s) 671
TaaI ACNGT 5 cut(s) 153, 292, 395, 549, 952
TaiI ACGT 5 cut(s) 258, 456, 632, 765, 938
TaqI TCGA 2 cut(s) 138, 942
TasI AATT 4 cut(s) 19, 82, 358, 503
TauI GCSGC 3 cut(s) 312, 479, 489
TfiI GAWTC 1 cut(s) 376
Tru1I TTAA 2 cut(s) 86, 885
Tru9I TTAA 2 cut(s) 86, 885
TscAI CASTG 3 cut(s) 295, 446, 706
TspDTI ATGAA 2 cut(s) 84, 371
TspGWI ACGGA 3 cut(s) 75, 117, 867
TspRI CASTG 3 cut(s) 295, 446, 706
VpaK11BI GGWCC 1 cut(s) 791
XapI RAATTY 1 cut(s) 82
XceI RCATGY 1 cut(s) 420
XmiI GTMKAC 1 cut(s) 616
XspI CTAG 3 cut(s) 219, 341, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.