pycom08g01700

Protein SHI RELATED SEQUENCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012802
Physical Location & Seq
Reverse (-)
1266498 .. 1268131
1634 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g01700.1

Sequence Viewer

Length: 1101 bp
ATGGCAGGCTTGTTTTGCTTAGGAGGGAGAGATCAAGGCCCAACCAGCGGTGACAAACAAGCAGGAGAGGATCAGAGCGGAAGAGTGGGAGGAGCAGCAGCGGAAGCGGCGGCAGGAGGAGGAGGAGGAGGAGGAGGGAACTTGTTTTTGTACAGATCATCAGGCGAGGAGCAGGCTGAGATCGACAACAAGGGGTTTGAGATATGGCCATCACAATACCACCCACACCAAAATTTGAACTACTACTCGTTTGGAGTGGATCCTAGCTACAACCATAGACACCTGGACAACAACAGCGACGGTGTTTCGGCGGATGATCTGTCGGCGGGGTTGAGGCTGACGATGATGAGAGGAGGAGGATTAGGAGGGTTGGGAAGCAGCAGCGGCGGCATGAACTGCCAGGACTGTGGGAACCAAGCAAAGAAAGACTGTCCGCACCTTAGGTGCCGGACCTGTTGCAAGAGCCGAGGGTTTCAGTGCCAAACACACGTGAAGAGCACGTGGGTTCCCGCTGCGAAACGACGGGAGCGGCAGGAACAATTCTCGGCCTTACAACAAAACCAAAACCAGCAACAACAGCAAGAACAGCAGATACAGTTTCGAGGTGAAAACCCCAAAAGGCTAAGAGATAACTCTCTTGCCTGCGTTCGTATTCCTTCCAACACGTCAGGGTTGGAGTTGAATCCATTTCCACCAGAAGTGAGCTCACCGGCGGTGTTCCGCTGCGTAAAAGTAAGTGCAATGGACGATGTAGATGAGCAATTCGCGTATCAAACGGCTGTCAACATCGGAGGGCATGTGTTCAAGGGACTTCTGTACGATCAGGGCATCGATGGTCAGTATTACCCAAGTTCTGGAGGAGGAGGTGGTGAGAGCTCCTCGGGCGGTCGAGGTACTAGTCATGATCACCACCAAGACGGATGTGGAAGCGGAGGAGGAGGAGGTCATCCACATCATCAGCAACATGATCTTGTAACGGTCGCAGCTGCCGGCGGGACCTCTGGCAATCCGTCTAGTACGTTGCTTGACCCTTCTCTCTTCCCAGCTCCACTCAATGCTTTCATGGCTGGTACGCAATACTTTCCGCCTCCGAGGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF702

Protein Analysis

367

Amino Acids

38.68

Weight (kDa)

6.14

Isoelectric Point (pI)

51.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF702 PF05142 130 - 278 4.7e-66 Domain of unknown function (DUF702)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012802)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19790 AT1G19790 AT1G75520
fragaria_vesca FvH4_2g38950
malus_domestica MD08G1021500.v1.1 MD15G1019200.v1.1
prunus_persica Prupe.1G371500_v2.0.a1 Prupe.1G371500_v2.0.a1
pyrus_communis pycom08g01700 pycom15g01600
rosa_chinensis RchiOBHm_Chr6g0302041
rosa_laevigata RLG00000011200
rosa_multiflora Rmu_ssc0000160.1_g000001
rosa_roxburghii Rroxscaffold_7G00166150
rosa_rugosa Rorug06G0311300
rosa_samantha Rh6AG424100 Rh6CG437100
rosa_wichuraiana Rw6G036730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 444
AccB7I CCANNNNNTGG 1 cut(s) 854
AccBSI CCGCTC 2 cut(s) 78, 529
AccII CGCG 1 cut(s) 767
AclWI GGATC 3 cut(s) 78, 254, 267
AcoI YGGCCR 1 cut(s) 206
AcsI RAATTY 1 cut(s) 232
AcvI CACGTG 2 cut(s) 490, 501
AfaI GTAC 5 cut(s) 152, 818, 895, 1018, 1072
AfiI CCNNNNNNNGG 2 cut(s) 47, 854
AflIII ACRYGT 2 cut(s) 487, 663
AgsI TTSAA 3 cut(s) 238, 682, 805
AhlI ACTAGT 1 cut(s) 896
AjiI CACGTC 1 cut(s) 666
AjnI CCWGG 2 cut(s) 282, 399
AluBI AGCT 5 cut(s) 267, 705, 876, 986, 1046
AluI AGCT 5 cut(s) 267, 705, 876, 986, 1046
Alw21I GWGCWC 3 cut(s) 500, 707, 878
AlwI GGATC 3 cut(s) 78, 254, 267
Ama87I CYCGRG 1 cut(s) 880
AoxI GGCC 3 cut(s) 37, 206, 546
ApeKI GCWGC 8 cut(s) 95, 98, 378, 381, 512, 723, 983, 986
ApoI RAATTY 1 cut(s) 232
AspS9I GGNCC 3 cut(s) 38, 450, 996
AsuHPI GGTGA 5 cut(s) 62, 617, 699, 881, 899
AvaI CYCGRG 1 cut(s) 880
AvaII GGWCC 2 cut(s) 450, 996
AxyI CCTNAGG 1 cut(s) 440
BalI TGGCCA 1 cut(s) 208
BamHI GGATCC 1 cut(s) 259
BanI GGYRCC 1 cut(s) 444
BanII GRGCYC 2 cut(s) 707, 878
BbrPI CACGTG 2 cut(s) 490, 501
Bbv12I GWGCWC 3 cut(s) 500, 707, 878
BbvI GCAGC 8 cut(s) 107, 110, 390, 393, 499, 710, 973, 995
BccI CCATC 2 cut(s) 217, 827
BceAI ACGGC 1 cut(s) 792
BciT130I CCWGG 2 cut(s) 284, 401
BclI TGATCA 1 cut(s) 904
BcuI ACTAGT 1 cut(s) 896
BfaI CTAG 3 cut(s) 264, 897, 1014
Bme1390I CCNGG 2 cut(s) 284, 401
Bme18I GGWCC 2 cut(s) 450, 996
BmeT110I CYCGRG 1 cut(s) 880
BmgBI CACGTC 1 cut(s) 666
BmgT120I GGNCC 3 cut(s) 38, 450, 996
BmiI GGNNCC 5 cut(s) 261, 413, 446, 507, 997
BmrFI CCNGG 2 cut(s) 284, 401
BmsI GCATC 1 cut(s) 837
BplI GAGNNNNNCTC 2 cut(s) 863, 895
BpmI CTGGAG 1 cut(s) 876
Bpu10I CCTNAGC 1 cut(s) 19
Bsa29I ATCGAT 1 cut(s) 831
BsaAI YACGTR 2 cut(s) 490, 501
BsaJI CCNNGG 3 cut(s) 466, 879, 1091
BsaXI ACNNNNNCTCC 2 cut(s) 783, 813
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 854
Bse118I RCCGGY 2 cut(s) 709, 989
Bse21I CCTNAGG 1 cut(s) 440
Bse3DI GCAATG 1 cut(s) 747
BseBI CCWGG 2 cut(s) 284, 401
BseCI ATCGAT 1 cut(s) 831
BseDI CCNNGG 3 cut(s) 466, 879, 1091
BseGI GGATG 3 cut(s) 319, 926, 946
BseLI CCNNNNNNNGG 2 cut(s) 47, 854
BseMI GCAATG 1 cut(s) 747
BseMII CTCAG 1 cut(s) 168
BseXI GCAGC 8 cut(s) 107, 110, 390, 393, 499, 710, 973, 995
BseYI CCCAGC 1 cut(s) 1042
Bsh1236I CGCG 1 cut(s) 767
Bsh1285I CGRYCG 2 cut(s) 889, 981
BshFI GGCC 3 cut(s) 39, 208, 548
BshNI GGYRCC 1 cut(s) 444
BshVI ATCGAT 1 cut(s) 831
BsiEI CGRYCG 2 cut(s) 889, 981
BsiHKAI GWGCWC 3 cut(s) 500, 707, 878
BsiHKCI CYCGRG 1 cut(s) 880
BsiSI CCGG 3 cut(s) 448, 710, 990
BslFI GGGAC 2 cut(s) 822, 1009
BslI CCNNNNNNNGG 2 cut(s) 47, 854
BsmFI GGGAC 2 cut(s) 822, 1009
BsnI GGCC 3 cut(s) 39, 208, 548
BsoBI CYCGRG 1 cut(s) 880
Bsp1286I GDGCHC 3 cut(s) 500, 707, 878
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 9 cut(s) 31, 70, 155, 180, 259, 316, 820, 904, 967
BspANI GGCC 3 cut(s) 39, 208, 548
BspCNI CTCAG 1 cut(s) 169
BspDI ATCGAT 1 cut(s) 831
BspFNI CGCG 1 cut(s) 767
BspHI TCATGA 1 cut(s) 901
BspLI GGNNCC 5 cut(s) 261, 413, 446, 507, 997
BspPI GGATC 3 cut(s) 78, 254, 267
BspQI GCTCTTC 1 cut(s) 488
BspT107I GGYRCC 1 cut(s) 444
BsrBI CCGCTC 2 cut(s) 78, 529
BsrDI GCAATG 1 cut(s) 747
BsrFI RCCGGY 2 cut(s) 709, 989
BsrGI TGTACA 1 cut(s) 150
BssAI RCCGGY 2 cut(s) 709, 989
BssECI CCNNGG 3 cut(s) 466, 879, 1091
BssMI GATC 9 cut(s) 31, 70, 155, 180, 259, 316, 820, 904, 967
Bst2UI CCWGG 2 cut(s) 284, 401
Bst4CI ACNGT 5 cut(s) 302, 407, 431, 597, 979
Bst6I CTCTTC 3 cut(s) 76, 488, 1043
BstAPI GCANNNNNTGC 1 cut(s) 396
BstAUI TGTACA 1 cut(s) 150
BstBAI YACGTR 2 cut(s) 490, 501
BstC8I GCNNGC 4 cut(s) 7, 174, 643, 991
BstDEI CTNAG 4 cut(s) 19, 177, 440, 623
BstF5I GGATG 3 cut(s) 319, 926, 946
BstFNI CGCG 1 cut(s) 767
BstKTI GATC 9 cut(s) 34, 73, 158, 183, 262, 319, 823, 907, 970
BstMBI GATC 9 cut(s) 31, 70, 155, 180, 259, 316, 820, 904, 967
BstMCI CGRYCG 2 cut(s) 889, 981
BstNI CCWGG 2 cut(s) 284, 401
BstNSI RCATGY 1 cut(s) 800
BstSCI CCNGG 2 cut(s) 282, 399
BstUI CGCG 1 cut(s) 767
BstV1I GCAGC 8 cut(s) 107, 110, 390, 393, 499, 710, 973, 995
BstX2I RGATCY 1 cut(s) 259
BstXI CCANNNNNNTGG 1 cut(s) 407
BstYI RGATCY 1 cut(s) 259
Bsu15I ATCGAT 1 cut(s) 831
Bsu36I CCTNAGG 1 cut(s) 440
BsuRI GGCC 3 cut(s) 39, 208, 548
BsuTUI ATCGAT 1 cut(s) 831
BtrI CACGTC 1 cut(s) 666
BtsCI GGATG 3 cut(s) 319, 926, 946
BtsIMutI CAGTG 1 cut(s) 482
Cac8I GCNNGC 4 cut(s) 7, 174, 643, 991
CciI TCATGA 1 cut(s) 901
Cfr10I RCCGGY 2 cut(s) 709, 989
Cfr13I GGNCC 3 cut(s) 38, 450, 996
ClaI ATCGAT 1 cut(s) 831
Csp6I GTAC 5 cut(s) 151, 817, 894, 1017, 1071
CviAII CATG 5 cut(s) 391, 797, 902, 965, 1063
CviQI GTAC 5 cut(s) 151, 817, 894, 1017, 1071
DdeI CTNAG 4 cut(s) 19, 177, 440, 623
DpnI GATC 9 cut(s) 33, 72, 157, 182, 261, 318, 822, 906, 969
DpnII GATC 9 cut(s) 31, 70, 155, 180, 259, 316, 820, 904, 967
EaeI YGGCCR 1 cut(s) 206
Eam1104I CTCTTC 3 cut(s) 76, 488, 1043
EarI CTCTTC 3 cut(s) 76, 488, 1043
EciI GGCGGA 2 cut(s) 326, 1074
Ecl136II GAGCTC 2 cut(s) 705, 876
Eco24I GRGCYC 2 cut(s) 707, 878
Eco47I GGWCC 2 cut(s) 450, 996
Eco53kI GAGCTC 2 cut(s) 705, 876
Eco72I CACGTG 2 cut(s) 490, 501
Eco81I CCTNAGG 1 cut(s) 440
Eco88I CYCGRG 1 cut(s) 880
EcoICRI GAGCTC 2 cut(s) 705, 876
EcoO109I RGGNCCY 1 cut(s) 996
EcoRII CCWGG 2 cut(s) 282, 399
EcoT38I GRGCYC 2 cut(s) 707, 878
FaeI CATG 5 cut(s) 394, 800, 905, 968, 1066
FaiI YATR 7 cut(s) 205, 276, 392, 798, 903, 966, 1064
FaqI GGGAC 2 cut(s) 822, 1009
FatI CATG 5 cut(s) 390, 796, 901, 964, 1062
FauI CCCGC 3 cut(s) 319, 517, 986
FbaI TGATCA 1 cut(s) 904
FokI GGATG 3 cut(s) 326, 933, 933
FriOI GRGCYC 2 cut(s) 707, 878
FspBI CTAG 3 cut(s) 264, 897, 1014
GsaI CCCAGC 1 cut(s) 1046
GsuI CTGGAG 1 cut(s) 876
HaeIII GGCC 3 cut(s) 39, 208, 548
HapII CCGG 3 cut(s) 448, 710, 990
Hin1II CATG 5 cut(s) 394, 800, 905, 968, 1066
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HinfI GANTC 1 cut(s) 682
HpaII CCGG 3 cut(s) 448, 710, 990
HphI GGTGA 5 cut(s) 62, 617, 699, 881, 899
Hpy166II GTNNAC 1 cut(s) 784
Hpy188I TCNGA 3 cut(s) 75, 791, 1092
Hpy188III TCNNGA 2 cut(s) 855, 902
Hpy8I GTNNAC 1 cut(s) 784
Hpy99I CGWCG 2 cut(s) 302, 525
HpyAV CCTTC 2 cut(s) 666, 1041
HpyCH4III ACNGT 5 cut(s) 302, 407, 431, 597, 979
HpyCH4IV ACGT 4 cut(s) 489, 500, 665, 1019
HpyCH4V TGCA 2 cut(s) 459, 740
HpyF3I CTNAG 4 cut(s) 19, 177, 440, 623
HpySE526I ACGT 4 cut(s) 489, 500, 665, 1019
Hsp92II CATG 5 cut(s) 394, 800, 905, 968, 1066
KroI GCCGGC 1 cut(s) 989
KroNI GCCGGC 1 cut(s) 991
Ksp22I TGATCA 1 cut(s) 904
Kzo9I GATC 9 cut(s) 31, 70, 155, 180, 259, 316, 820, 904, 967
LguI GCTCTTC 1 cut(s) 488
LmnI GCTCC 5 cut(s) 92, 169, 526, 881, 1051
Lsp1109I GCAGC 8 cut(s) 107, 110, 390, 393, 499, 710, 973, 995
LweI GCATC 1 cut(s) 837
MaeI CTAG 3 cut(s) 264, 897, 1014
MaeII ACGT 4 cut(s) 489, 500, 665, 1019
MaeIII GTNAC 2 cut(s) 50, 973
MalI GATC 9 cut(s) 33, 72, 157, 182, 261, 318, 822, 906, 969
MbiI CCGCTC 2 cut(s) 78, 529
MboI GATC 9 cut(s) 31, 70, 155, 180, 259, 316, 820, 904, 967
MboII GAAGA 3 cut(s) 93, 505, 1030
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 3 cut(s) 500, 707, 878
MlsI TGGCCA 1 cut(s) 208
MluCI AATT 3 cut(s) 232, 539, 761
MluNI TGGCCA 1 cut(s) 208
MmeI TCCRAC 2 cut(s) 654, 684
Mox20I TGGCCA 1 cut(s) 208
MroNI GCCGGC 1 cut(s) 989
MscI TGGCCA 1 cut(s) 208
MseI TTAA 1 cut(s) 1099
Msp20I TGGCCA 1 cut(s) 208
MspA1I CMGCKG 6 cut(s) 48, 101, 384, 512, 723, 986
MspI CCGG 3 cut(s) 448, 710, 990
MspR9I CCNGG 2 cut(s) 284, 401
MvaI CCWGG 2 cut(s) 284, 401
MvnI CGCG 1 cut(s) 767
NaeI GCCGGC 1 cut(s) 991
NdeII GATC 9 cut(s) 31, 70, 155, 180, 259, 316, 820, 904, 967
NgoMIV GCCGGC 1 cut(s) 989
NlaIII CATG 5 cut(s) 394, 800, 905, 968, 1066
NlaIV GGNNCC 5 cut(s) 261, 413, 446, 507, 997
NmeAIII GCCGAG 2 cut(s) 491, 524
NmuCI GTSAC 1 cut(s) 50
NspI RCATGY 1 cut(s) 800
PagI TCATGA 1 cut(s) 901
PciSI GCTCTTC 1 cut(s) 488
PdiI GCCGGC 1 cut(s) 991
PfeI GAWTC 1 cut(s) 682
PflMI CCANNNNNTGG 1 cut(s) 854
PmaCI CACGTG 2 cut(s) 490, 501
PmlI CACGTG 2 cut(s) 490, 501
Ppu21I YACGTR 2 cut(s) 490, 501
PpuMI RGGWCCY 1 cut(s) 996
Psp124BI GAGCTC 2 cut(s) 707, 878
Psp5II RGGWCCY 1 cut(s) 996
Psp6I CCWGG 2 cut(s) 282, 399
PspCI CACGTG 2 cut(s) 490, 501
PspFI CCCAGC 1 cut(s) 1042
PspGI CCWGG 2 cut(s) 282, 399
PspN4I GGNNCC 5 cut(s) 261, 413, 446, 507, 997
PspPI GGNCC 3 cut(s) 38, 450, 996
PspPPI RGGWCCY 1 cut(s) 996
PsrI GAACNNNNNNTAC 2 cut(s) 576, 608
PsuI RGATCY 1 cut(s) 259
PvuII CAGCTG 1 cut(s) 986
RsaI GTAC 5 cut(s) 152, 818, 895, 1018, 1072
RsaNI GTAC 5 cut(s) 151, 817, 894, 1017, 1071
SacI GAGCTC 2 cut(s) 707, 878
SapI GCTCTTC 1 cut(s) 488
SaqAI TTAA 1 cut(s) 1099
Sau3AI GATC 9 cut(s) 31, 70, 155, 180, 259, 316, 820, 904, 967
Sau96I GGNCC 3 cut(s) 38, 450, 996
ScrFI CCNGG 2 cut(s) 284, 401
SduI GDGCHC 3 cut(s) 500, 707, 878
SfaNI GCATC 1 cut(s) 837
SgrAI CRCCGGYG 1 cut(s) 709
SinI GGWCC 2 cut(s) 450, 996
SpeI ACTAGT 1 cut(s) 896
Sse9I AATT 3 cut(s) 232, 539, 761
SspMI CTAG 3 cut(s) 264, 897, 1014
SstI GAGCTC 2 cut(s) 707, 878
StyD4I CCNGG 2 cut(s) 282, 399
TaaI ACNGT 5 cut(s) 302, 407, 431, 597, 979
TaiI ACGT 4 cut(s) 492, 503, 668, 1022
TaqI TCGA 4 cut(s) 183, 601, 831, 889
TasI AATT 3 cut(s) 232, 539, 761
TatI WGTACW 1 cut(s) 150
TauI GCSGC 5 cut(s) 110, 113, 387, 390, 532
TfiI GAWTC 1 cut(s) 682
Tru1I TTAA 1 cut(s) 1099
Tru9I TTAA 1 cut(s) 1099
TscAI CASTG 1 cut(s) 482
TseFI GTSAC 1 cut(s) 50
TseI GCWGC 8 cut(s) 95, 98, 378, 381, 512, 723, 983, 986
Tsp45I GTSAC 1 cut(s) 50
TspDTI ATGAA 2 cut(s) 407, 1051
TspGWI ACGGA 2 cut(s) 933, 999
TspRI CASTG 1 cut(s) 482
Van91I CCANNNNNTGG 1 cut(s) 854
VpaK11BI GGWCC 2 cut(s) 450, 996
XapI RAATTY 1 cut(s) 232
XceI RCATGY 1 cut(s) 800
XcmI CCANNNNNNNNNTGG 1 cut(s) 920
XspI CTAG 3 cut(s) 264, 897, 1014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.