FvH4_2g40160

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0012176
Physical Location & Seq
Forward (+)
28676430 .. 28678591
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40160.t1

Sequence Viewer

Length: 957 bp
ATGGCAATGTCTGTGGCCACGAAGCTCCAATCCTCAATTTGCGAGCCTCGCGCCGTTCTCGGTCCTGGTGGGAACAGAGTCAGGGTTTCGGAGGGACCTAAACAAAAGTGTGAGGGGCTCAAGAAGCCGCCGCAGAGGCCGAGAAAACCGGCCCCGGAGATTCCGGTGTCGGTTGTGAAGAAAAACGTCTCCGTGGACAGCTCGTGTTCTTCGGATACCTCTTCAACTGGCTCATCTCCCAAGACGGCGAGTCCTAGAAAGACCGTAAGGCATATCAAGAGATTCAGGCCTCCCAAGCTTGTCTCAAACGACGTCGTCAAGCCCGACGGTCCACCTAAGCGATGTGAATGGATTACAGCAAACTCCGACCCACATTACATTTCTTTCCACGATGAAGAATGGGGAGTTCCGGTTTATGATGATGAGAAGCTGTTTGAACTGCTAGTGCTTTCGCAATCTCTAGCAGAACTTAGTTGGCCTGAAATTCTTACCAAGAGGGACATATTCAGGAATCTTTTTGATAACTTTGATCCTTCATCCATTGCAAAGTTTGATGAGAACAAGTTGCTGTCACTTAAAATAAATGGCATCCCAATTCTATCCCAACAAAAGCTTCGTGCAATCGTGGACAATGCTATTCAAGTGGTGAAGATTCAGCAAGAGTTTGGTTCCTTCAGTAAATACTGCTGGAGCTTTGTCAACCAGAAGGCGATCAACAATGGAGTTCGTTATGGACGCCAAGTACCAGCAAAGTCGCCAAAAGCTGAAGTTATAAGCAAGGATCTGATGAAGAGAGGCTTCCGTTGTGTTGGTCCTACCGTCGTGTATTCATTCATGCAAGTAGCTGGGATTGTGAACGATCATCTTGTAACATGCTTCCGGCACAAGGAGTGCGATGCTAATGCGAAGACGGATGTGAAACTTGAGAATGAGAAGTCCAAAGATATACTAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

319

Amino Acids

35.59

Weight (kDa)

9.49

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 120 - 295 8.8e-68 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15970 AT1G15970 AT1G75090 AT1G80850
fragaria_vesca FvH4_2g40160
malus_domestica MD08G1035300.v1.1
prunus_persica Prupe.1G382800_v2.0.a1
pyrus_communis pycom08g02790
rosa_chinensis RchiOBHm_Chr6g0300571
rosa_laevigata RLG00000011323
rosa_multiflora Rmu_sc0021182.1_g000001 Rmu_sc0026329.1_g000001
rosa_roxburghii Rroxscaffold_7G00167660
rosa_rugosa Rorug06G0297300
rosa_samantha Rh6AG409800 Rh6BG416900 Rh6CG424800 Rh6DG410300
rosa_wichuraiana Rw6G035860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 773
AatII GACGTC 1 cut(s) 315
AccII CGCG 1 cut(s) 51
AciI CCGC 2 cut(s) 128, 131
AclWI GGATC 2 cut(s) 524, 789
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 1 cut(s) 483
AcuI CTGAAG 2 cut(s) 658, 786
AcyI GRCGYC 2 cut(s) 312, 736
AfaI GTAC 1 cut(s) 744
AfiI CCNNNNNNNGG 1 cut(s) 886
AgsI TTSAA 3 cut(s) 225, 437, 641
AhdI GACNNNNNGTC 1 cut(s) 249
AjnI CCWGG 1 cut(s) 64
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AluBI AGCT 8 cut(s) 25, 201, 298, 430, 613, 693, 764, 845
AluI AGCT 8 cut(s) 25, 201, 298, 430, 613, 693, 764, 845
Alw26I GTCTC 2 cut(s) 193, 307
AlwI GGATC 2 cut(s) 524, 789
AoxI GGCC 5 cut(s) 15, 137, 150, 287, 476
ApoI RAATTY 1 cut(s) 483
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 5 cut(s) 62, 95, 151, 329, 812
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 1 cut(s) 658
AvaII GGWCC 4 cut(s) 62, 95, 329, 812
BalI TGGCCA 1 cut(s) 17
BanII GRGCYC 1 cut(s) 120
BauI CACGAG 1 cut(s) 202
BbsI GAAGAC 1 cut(s) 914
BceAI ACGGC 2 cut(s) 38, 261
BcgI CGANNNNNNTGC 2 cut(s) 884, 918
BciT130I CCWGG 1 cut(s) 66
BciVI GTATCC 1 cut(s) 208
BcnI CCSGG 1 cut(s) 155
BcoDI GTCTC 2 cut(s) 193, 307
BfaI CTAG 3 cut(s) 255, 443, 461
BfuI GTATCC 1 cut(s) 208
BglI GCCNNNNNGGC 1 cut(s) 136
BisI GCNGC 2 cut(s) 128, 131
BlsI GCNGC 2 cut(s) 129, 132
Bme1390I CCNGG 2 cut(s) 66, 155
Bme18I GGWCC 4 cut(s) 62, 95, 329, 812
BmeRI GACNNNNNGTC 1 cut(s) 249
BmgT120I GGNCC 5 cut(s) 62, 95, 151, 329, 812
BmiI GGNNCC 3 cut(s) 96, 153, 670
BmrFI CCNGG 2 cut(s) 66, 155
BmsI GCATC 2 cut(s) 597, 886
BpiI GAAGAC 1 cut(s) 914
BpmI CTGGAG 1 cut(s) 709
Bpu10I CCTNAGC 1 cut(s) 336
BpuEI CTTGAG 2 cut(s) 104, 944
BpuMI CCSGG 1 cut(s) 155
BsaHI GRCGYC 2 cut(s) 312, 736
BsaJI CCNNGG 2 cut(s) 153, 192
BsaWI WCCGGW 2 cut(s) 163, 409
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bsc4I CCNNNNNNNGG 1 cut(s) 886
Bse118I RCCGGY 1 cut(s) 148
Bse1I ACTGG 1 cut(s) 232
Bse3DI GCAATG 2 cut(s) 12, 540
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 153, 192
BseGI GGATG 3 cut(s) 536, 588, 919
BseLI CCNNNNNNNGG 1 cut(s) 886
BseMI GCAATG 2 cut(s) 12, 540
BseNI ACTGG 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 845
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 5 cut(s) 17, 139, 152, 289, 478
BsiSI CCGG 5 cut(s) 149, 155, 164, 410, 880
BslFI GGGAC 2 cut(s) 108, 512
BslI CCNNNNNNNGG 1 cut(s) 886
BsmAI GTCTC 2 cut(s) 193, 307
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 2 cut(s) 108, 512
BsnI GGCC 5 cut(s) 17, 139, 152, 289, 478
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 4 cut(s) 529, 711, 781, 859
BspACI CCGC 2 cut(s) 128, 131
BspANI GGCC 5 cut(s) 17, 139, 152, 289, 478
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 3 cut(s) 96, 153, 670
BspPI GGATC 2 cut(s) 524, 789
BsrDI GCAATG 2 cut(s) 12, 540
BsrFI RCCGGY 1 cut(s) 148
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 2 cut(s) 153, 192
BssMI GATC 4 cut(s) 529, 711, 781, 859
BssNI GRCGYC 2 cut(s) 312, 736
BssSI CACGAG 1 cut(s) 202
Bst2BI CACGAG 1 cut(s) 202
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 3 cut(s) 265, 329, 820
Bst6I CTCTTC 2 cut(s) 226, 785
BstACI GRCGYC 2 cut(s) 312, 736
BstC8I GCNNGC 1 cut(s) 44
BstDEI CTNAG 2 cut(s) 336, 470
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 3 cut(s) 536, 588, 919
BstFNI CGCG 1 cut(s) 51
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 4 cut(s) 532, 714, 784, 862
BstMAI GTCTC 2 cut(s) 193, 307
BstMBI GATC 4 cut(s) 529, 711, 781, 859
BstMWI GCNNNNNNNGC 4 cut(s) 48, 124, 136, 295
BstNI CCWGG 1 cut(s) 66
BstNSI RCATGY 1 cut(s) 876
BstSCI CCNGG 2 cut(s) 64, 153
BstUI CGCG 1 cut(s) 51
BstV2I GAAGAC 1 cut(s) 914
BstX2I RGATCY 1 cut(s) 781
BstYI RGATCY 1 cut(s) 781
BsuI GTATCC 1 cut(s) 208
BsuRI GGCC 5 cut(s) 17, 139, 152, 289, 478
BtgI CCRYGG 1 cut(s) 192
BtgZI GCGATG 2 cut(s) 355, 909
BtsCI GGATG 3 cut(s) 536, 588, 919
Cac8I GCNNGC 1 cut(s) 44
CfoI GCGC 1 cut(s) 53
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 5 cut(s) 62, 95, 151, 329, 812
CseI GACGC 1 cut(s) 744
Csp6I GTAC 1 cut(s) 743
CspCI CAANNNNNGTGG 1 cut(s) 29
CviAII CATG 2 cut(s) 835, 873
CviQI GTAC 1 cut(s) 743
DdeI CTNAG 2 cut(s) 336, 470
DpnI GATC 4 cut(s) 531, 713, 783, 861
DpnII GATC 4 cut(s) 529, 711, 781, 859
DriI GACNNNNNGTC 1 cut(s) 249
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 2 cut(s) 226, 785
Eam1105I GACNNNNNGTC 1 cut(s) 249
EarI CTCTTC 2 cut(s) 226, 785
Eco147I AGGCCT 1 cut(s) 289
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 4 cut(s) 62, 95, 329, 812
Eco57I CTGAAG 2 cut(s) 658, 786
EcoO109I RGGNCCY 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 120
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 2 cut(s) 838, 876
FaiI YATR 8 cut(s) 273, 417, 503, 732, 773, 836, 874, 947
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FaqI GGGAC 2 cut(s) 108, 512
FatI CATG 2 cut(s) 834, 872
Fnu4HI GCNGC 2 cut(s) 128, 131
FokI GGATG 3 cut(s) 523, 575, 926
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 2 cut(s) 128, 131
FspBI CTAG 3 cut(s) 255, 443, 461
GlaI GCGC 1 cut(s) 52
GluI GCNGC 2 cut(s) 128, 131
GsaI CCCAGC 1 cut(s) 849
GsuI CTGGAG 1 cut(s) 709
HaeIII GGCC 5 cut(s) 17, 139, 152, 289, 478
HapII CCGG 5 cut(s) 149, 155, 164, 410, 880
HgaI GACGC 1 cut(s) 744
HhaI GCGC 1 cut(s) 53
Hin1I GRCGYC 2 cut(s) 312, 736
Hin1II CATG 2 cut(s) 838, 876
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HincII GTYRAC 1 cut(s) 700
HindII GTYRAC 1 cut(s) 700
HindIII AAGCTT 2 cut(s) 296, 611
HinfI GANTC 6 cut(s) 78, 160, 250, 282, 511, 652
HpaII CCGG 5 cut(s) 149, 155, 164, 410, 880
HphI GGTGA 1 cut(s) 658
Hpy166II GTNNAC 5 cut(s) 196, 332, 628, 700, 856
Hpy188I TCNGA 4 cut(s) 91, 214, 367, 786
Hpy188III TCNNGA 3 cut(s) 121, 277, 508
Hpy8I GTNNAC 5 cut(s) 196, 332, 628, 700, 856
Hpy99I CGWCG 4 cut(s) 314, 317, 329, 824
HpyAV CCTTC 3 cut(s) 543, 682, 700
HpyCH4III ACNGT 3 cut(s) 265, 329, 820
HpyCH4IV ACGT 2 cut(s) 186, 312
HpyCH4V TGCA 3 cut(s) 545, 620, 838
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 124, 136, 295
HpyF3I CTNAG 2 cut(s) 336, 470
HpySE526I ACGT 2 cut(s) 186, 312
Hsp92I GRCGYC 2 cut(s) 312, 736
Hsp92II CATG 2 cut(s) 838, 876
HspAI GCGC 1 cut(s) 51
Kzo9I GATC 4 cut(s) 529, 711, 781, 859
LmnI GCTCC 2 cut(s) 30, 690
LweI GCATC 2 cut(s) 597, 886
MaeI CTAG 3 cut(s) 255, 443, 461
MaeII ACGT 2 cut(s) 186, 312
MaeIII GTNAC 2 cut(s) 570, 868
MalI GATC 4 cut(s) 531, 713, 783, 861
MboI GATC 4 cut(s) 529, 711, 781, 859
MboII GAAGA 7 cut(s) 190, 201, 213, 407, 661, 802, 919
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 1 cut(s) 120
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 3 cut(s) 36, 483, 594
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 2 cut(s) 87, 259
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 9 cut(s) 43, 57, 85, 106, 129, 229, 300, 489, 788
Mox20I TGGCCA 1 cut(s) 17
MscI TGGCCA 1 cut(s) 17
MseI TTAA 1 cut(s) 576
Msp20I TGGCCA 1 cut(s) 17
MspI CCGG 5 cut(s) 149, 155, 164, 410, 880
MspR9I CCNGG 2 cut(s) 66, 155
MvaI CCWGG 1 cut(s) 66
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 4 cut(s) 48, 124, 136, 295
NciI CCSGG 1 cut(s) 155
NdeII GATC 4 cut(s) 529, 711, 781, 859
NlaIII CATG 2 cut(s) 838, 876
NlaIV GGNNCC 3 cut(s) 96, 153, 670
NmeAIII GCCGAG 1 cut(s) 165
NmuCI GTSAC 1 cut(s) 570
NspI RCATGY 1 cut(s) 876
PceI AGGCCT 1 cut(s) 289
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PfeI GAWTC 4 cut(s) 160, 282, 511, 652
PflFI GACNNNGTC 1 cut(s) 314
PkrI GCNGC 2 cut(s) 129, 132
PleI GAGTC 2 cut(s) 86, 258
PpsI GAGTC 2 cut(s) 86, 258
PpuMI RGGWCCY 1 cut(s) 95
PsiI TTATAA 1 cut(s) 773
Psp5II RGGWCCY 1 cut(s) 95
Psp6I CCWGG 1 cut(s) 64
PspFI CCCAGC 1 cut(s) 845
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 3 cut(s) 96, 153, 670
PspPI GGNCC 5 cut(s) 62, 95, 151, 329, 812
PspPPI RGGWCCY 1 cut(s) 95
PsuI RGATCY 1 cut(s) 781
PsyI GACNNNGTC 1 cut(s) 314
RsaI GTAC 1 cut(s) 744
RsaNI GTAC 1 cut(s) 743
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 2 cut(s) 128, 131
Sau3AI GATC 4 cut(s) 529, 711, 781, 859
Sau96I GGNCC 5 cut(s) 62, 95, 151, 329, 812
SchI GAGTC 2 cut(s) 87, 259
ScrFI CCNGG 2 cut(s) 66, 155
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 2 cut(s) 597, 886
SinI GGWCC 4 cut(s) 62, 95, 329, 812
SmlI CTYRAG 2 cut(s) 119, 923
SmoI CTYRAG 2 cut(s) 119, 923
Sse9I AATT 3 cut(s) 36, 483, 594
SseBI AGGCCT 1 cut(s) 289
SsiI CCGC 2 cut(s) 128, 131
SspMI CTAG 3 cut(s) 255, 443, 461
StuI AGGCCT 1 cut(s) 289
StyD4I CCNGG 2 cut(s) 64, 153
TaaI ACNGT 3 cut(s) 265, 329, 820
TaiI ACGT 2 cut(s) 189, 315
TaqII GACCGA 1 cut(s) 50
TasI AATT 3 cut(s) 36, 483, 594
TauI GCSGC 2 cut(s) 130, 133
TfiI GAWTC 4 cut(s) 160, 282, 511, 652
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TseFI GTSAC 1 cut(s) 570
Tsp45I GTSAC 1 cut(s) 570
TspDTI ATGAA 5 cut(s) 408, 525, 803, 819, 823
TspGWI ACGGA 3 cut(s) 181, 791, 926
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 4 cut(s) 62, 95, 329, 812
XapI RAATTY 1 cut(s) 483
XceI RCATGY 1 cut(s) 876
XspI CTAG 3 cut(s) 255, 443, 461
ZraI GACGTC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.