pycom08g02790

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0012176
Physical Location & Seq
Forward (+)
2032300 .. 2033882
1583 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g02790.3

Sequence Viewer

Length: 993 bp
ATGGCAATGTCGGTGGCCAGTAAGCTTCAATCTCCGACTTGCGAGCCACGAGCCGTACTCGGTCCCGGCGGGAACAGAGTTAGGGTTTTGGAGGGCCCGAAACGCAAAGACGACGGCGCGAAGAAGCCGCCGCAGCAGACACCGAGGCCGAGAAAACCTGTACCTGAATTGCCGGAACCGGTGGTGCGGAGCGACGTTTCGGTGGACAGCACGTGCTCTTCGGACACGTCGTCGAGCGGTTACTCTGCGAAGACGGCGAGCCCTGGAAAGATTGTGAGGCGTAAGAGCTTGAGGCCGGCGAAGGTTGCTTCCGACGATGCCGAGGTGGCCAAGCCCGCCGGTCCGCTGAAACGGTGCGATTGGATTACACCTCACGCTGATCCGATTTATACTTGTTTCCACGACGAAGAATGGGGGGTTCCAGTGTATGACGATAAGAAGCTGTTTGAACTGCTTGTCCTTTCACAAGCTCTCGCGGAACTCAGCTGGCCGGAAATACTACGCAAGAGAGATTTATTCAGGAAACTTTTTGACAATTTTGATCCGTCCTCCATTGCAAAGTTTGATGAGAAGAAATTGCTGTCTCTCAAAGTGCTGTCCGAACAAAAGCTTCGTGCAATTGACGAGAATGCCAAACAAATGCTCAAGGTTCAGCAGGAGTTTGGTTCCTTCAGTAACTACTGCTGGAGCTTTGTGAACCACAAGCCCGTAAGAAATGGATTCCGTTATGCACGTCAAGTGCCGGTGAAGTCCCCAAAAGCTGAAGCCATAAGCAAAGATTTGATGAAGAGAGGCTTCCGCTGTGTCGGGCCTACTGTCATTTACTCATTTATGCAAGTGGCGGGGATTGTGAACGATCATCTCGCAACATGCTACCGGTTCAAGGAATGCAACGCAAATGCCAAAGCAGACTCAAAACATAAGATTGAGAAGAAGACAGGAGAAGATGACACTAAACTAACCGAAGCTATGAAGATCGGAGACCAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

331

Amino Acids

36.95

Weight (kDa)

9.42

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15970 AT1G15970 AT1G75090 AT1G80850
fragaria_vesca FvH4_2g40160
malus_domestica MD08G1035300.v1.1
prunus_persica Prupe.1G382800_v2.0.a1
pyrus_communis pycom08g02790
rosa_chinensis RchiOBHm_Chr6g0300571
rosa_laevigata RLG00000011323
rosa_multiflora Rmu_sc0021182.1_g000001 Rmu_sc0026329.1_g000001
rosa_roxburghii Rroxscaffold_7G00167660
rosa_rugosa Rorug06G0297300
rosa_samantha Rh6AG409800 Rh6BG416900 Rh6CG424800 Rh6DG410300
rosa_wichuraiana Rw6G035860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 237
AccII CGCG 2 cut(s) 119, 476
AclWI GGATC 2 cut(s) 374, 536
AcoI YGGCCR 3 cut(s) 15, 327, 488
AcuI CTGAAG 2 cut(s) 655, 783
AcvI CACGTG 1 cut(s) 213
AfaI GTAC 2 cut(s) 57, 162
AfiI CCNNNNNNNGG 1 cut(s) 883
AflIII ACRYGT 1 cut(s) 225
AgeI ACCGGT 2 cut(s) 178, 876
AgsI TTSAA 3 cut(s) 29, 449, 883
AhdI GACNNNNNGTC 1 cut(s) 229
AjiI CACGTC 2 cut(s) 228, 734
AjnI CCWGG 1 cut(s) 262
AloI GAACNNNNNNTCC 2 cut(s) 441, 473
AluBI AGCT 9 cut(s) 25, 288, 442, 470, 486, 610, 690, 761, 968
AluI AGCT 9 cut(s) 25, 288, 442, 470, 486, 610, 690, 761, 968
Alw21I GWGCWC 1 cut(s) 218
Alw26I GTCTC 2 cut(s) 588, 975
AlwI GGATC 2 cut(s) 374, 536
AoxI GGCC 7 cut(s) 15, 94, 146, 293, 327, 488, 809
ApaI GGGCCC 1 cut(s) 98
ApeKI GCWGC 1 cut(s) 133
AsiGI ACCGGT 2 cut(s) 178, 876
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 5 cut(s) 62, 94, 95, 341, 809
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 757
AvaII GGWCC 2 cut(s) 62, 341
BaeGI GKGCMC 1 cut(s) 98
BalI TGGCCA 2 cut(s) 17, 329
BanII GRGCYC 2 cut(s) 98, 263
BauI CACGAG 1 cut(s) 48
BbrPI CACGTG 1 cut(s) 213
BbsI GAAGAC 2 cut(s) 257, 941
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 1 cut(s) 145
BceAI ACGGC 3 cut(s) 38, 130, 270
BciT130I CCWGG 1 cut(s) 264
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 2 cut(s) 588, 975
BglI GCCNNNNNGGC 1 cut(s) 326
BisI GCNGC 3 cut(s) 128, 131, 134
BlsI GCNGC 3 cut(s) 129, 132, 135
Bme1390I CCNGG 2 cut(s) 66, 264
Bme18I GGWCC 2 cut(s) 62, 341
BmeRI GACNNNNNGTC 1 cut(s) 229
BmgBI CACGTC 2 cut(s) 228, 734
BmgT120I GGNCC 5 cut(s) 62, 94, 95, 341, 809
BmiI GGNNCC 5 cut(s) 64, 96, 177, 420, 667
BmrFI CCNGG 2 cut(s) 66, 264
BmsI GCATC 1 cut(s) 307
BpiI GAAGAC 2 cut(s) 257, 941
BplI GAGNNNNNCTC 2 cut(s) 42, 74
BpmI CTGGAG 1 cut(s) 706
BpuEI CTTGAG 2 cut(s) 310, 629
BpuMI CCSGG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 975
BsaJI CCNNGG 3 cut(s) 143, 262, 321
BsaWI WCCGGW 2 cut(s) 178, 876
Bsc4I CCNNNNNNNGG 1 cut(s) 883
Bse118I RCCGGY 5 cut(s) 178, 295, 338, 742, 876
Bse1I ACTGG 2 cut(s) 18, 422
Bse3DI GCAATG 2 cut(s) 12, 552
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 3 cut(s) 143, 262, 321
BseLI CCNNNNNNNGG 1 cut(s) 883
BseMI GCAATG 2 cut(s) 12, 552
BseMII CTCAG 1 cut(s) 496
BseNI ACTGG 2 cut(s) 18, 422
BseSI GKGCMC 1 cut(s) 98
BseXI GCAGC 1 cut(s) 145
Bsh1236I CGCG 2 cut(s) 119, 476
BshFI GGCC 7 cut(s) 17, 96, 148, 295, 329, 490, 811
BshTI ACCGGT 2 cut(s) 178, 876
BsiHKAI GWGCWC 1 cut(s) 218
BsiSI CCGG 8 cut(s) 66, 173, 179, 296, 339, 491, 743, 877
BslFI GGGAC 2 cut(s) 48, 736
BslI CCNNNNNNNGG 1 cut(s) 883
BsmAI GTCTC 2 cut(s) 588, 975
BsmFI GGGAC 2 cut(s) 48, 736
BsmI GAATGC 2 cut(s) 634, 893
BsnI GGCC 7 cut(s) 17, 96, 148, 295, 329, 490, 811
Bso31I GGTCTC 1 cut(s) 975
Bsp120I GGGCCC 1 cut(s) 94
Bsp1286I GDGCHC 3 cut(s) 98, 218, 263
Bsp143I GATC 4 cut(s) 379, 541, 856, 975
BspANI GGCC 7 cut(s) 17, 96, 148, 295, 329, 490, 811
BspCNI CTCAG 1 cut(s) 495
BspFNI CGCG 2 cut(s) 119, 476
BspLI GGNNCC 5 cut(s) 64, 96, 177, 420, 667
BspPI GGATC 2 cut(s) 374, 536
BspQI GCTCTTC 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 975
BsrBI CCGCTC 1 cut(s) 237
BsrDI GCAATG 2 cut(s) 12, 552
BsrFI RCCGGY 5 cut(s) 178, 295, 338, 742, 876
BsrI ACTGG 2 cut(s) 18, 422
BssAI RCCGGY 5 cut(s) 178, 295, 338, 742, 876
BssECI CCNNGG 3 cut(s) 143, 262, 321
BssMI GATC 4 cut(s) 379, 541, 856, 975
BssSI CACGAG 1 cut(s) 48
Bst2BI CACGAG 1 cut(s) 48
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 2 cut(s) 354, 817
Bst6I CTCTTC 2 cut(s) 223, 782
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 5 cut(s) 44, 259, 297, 336, 488
BstDEI CTNAG 1 cut(s) 482
BstFNI CGCG 2 cut(s) 119, 476
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 4 cut(s) 382, 544, 859, 978
BstMAI GTCTC 2 cut(s) 588, 975
BstMBI GATC 4 cut(s) 379, 541, 856, 975
BstMWI GCNNNNNNNGC 6 cut(s) 102, 133, 254, 305, 326, 335
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 873
BstSCI CCNGG 2 cut(s) 64, 262
BstSLI GKGCMC 1 cut(s) 98
BstUI CGCG 2 cut(s) 119, 476
BstV1I GCAGC 1 cut(s) 145
BstV2I GAAGAC 2 cut(s) 257, 941
BsuRI GGCC 7 cut(s) 17, 96, 148, 295, 329, 490, 811
BtrI CACGTC 2 cut(s) 228, 734
BtsIMutI CAGTG 1 cut(s) 429
Cac8I GCNNGC 5 cut(s) 44, 259, 297, 336, 488
CfoI GCGC 1 cut(s) 119
Cfr10I RCCGGY 5 cut(s) 178, 295, 338, 742, 876
Cfr13I GGNCC 5 cut(s) 62, 94, 95, 341, 809
CpoI CGGWCCG 1 cut(s) 341
Csp6I GTAC 2 cut(s) 56, 161
CspAI ACCGGT 2 cut(s) 178, 876
CspCI CAANNNNNGTGG 1 cut(s) 29
CspI CGGWCCG 1 cut(s) 341
CviAII CATG 1 cut(s) 870
CviQI GTAC 2 cut(s) 56, 161
DdeI CTNAG 1 cut(s) 482
DpnI GATC 4 cut(s) 381, 543, 858, 977
DpnII GATC 4 cut(s) 379, 541, 856, 975
DriI GACNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 3 cut(s) 15, 327, 488
Eam1104I CTCTTC 2 cut(s) 223, 782
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 2 cut(s) 223, 782
Eco24I GRGCYC 2 cut(s) 98, 263
Eco31I GGTCTC 1 cut(s) 975
Eco47I GGWCC 2 cut(s) 62, 341
Eco57I CTGAAG 2 cut(s) 655, 783
Eco72I CACGTG 1 cut(s) 213
EcoO109I RGGNCCY 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 262
EcoT38I GRGCYC 2 cut(s) 98, 263
FaeI CATG 1 cut(s) 873
FaiI YATR 8 cut(s) 390, 429, 729, 770, 833, 871, 921, 971
FalI AAGNNNNNCTT 2 cut(s) 779, 811
FaqI GGGAC 2 cut(s) 48, 736
FatI CATG 1 cut(s) 869
FauI CCCGC 3 cut(s) 62, 343, 835
Fnu4HI GCNGC 3 cut(s) 128, 131, 134
FriOI GRGCYC 2 cut(s) 98, 263
Fsp4HI GCNGC 3 cut(s) 128, 131, 134
GlaI GCGC 1 cut(s) 118
GluI GCNGC 3 cut(s) 128, 131, 134
GsuI CTGGAG 1 cut(s) 706
HaeIII GGCC 7 cut(s) 17, 96, 148, 295, 329, 490, 811
HapII CCGG 8 cut(s) 66, 173, 179, 296, 339, 491, 743, 877
HhaI GCGC 1 cut(s) 119
Hin1II CATG 1 cut(s) 873
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HindIII AAGCTT 2 cut(s) 23, 608
HinfI GANTC 2 cut(s) 720, 911
HpaII CCGG 8 cut(s) 66, 173, 179, 296, 339, 491, 743, 877
HphI GGTGA 1 cut(s) 757
Hpy166II GTNNAC 3 cut(s) 205, 697, 853
Hpy188I TCNGA 6 cut(s) 36, 223, 313, 384, 601, 980
Hpy188III TCNNGA 1 cut(s) 520
Hpy8I GTNNAC 3 cut(s) 205, 697, 853
Hpy99I CGWCG 6 cut(s) 116, 197, 232, 235, 317, 407
HpyAV CCTTC 2 cut(s) 295, 679
HpyCH4III ACNGT 2 cut(s) 354, 817
HpyCH4IV ACGT 4 cut(s) 195, 212, 227, 733
HpyCH4V TGCA 5 cut(s) 557, 617, 731, 835, 891
HpyF10VI GCNNNNNNNGC 6 cut(s) 102, 133, 254, 305, 326, 335
HpyF3I CTNAG 1 cut(s) 482
HpySE526I ACGT 4 cut(s) 195, 212, 227, 733
Hsp92II CATG 1 cut(s) 873
HspAI GCGC 1 cut(s) 117
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
Kzo9I GATC 4 cut(s) 379, 541, 856, 975
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 2 cut(s) 189, 687
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 1 cut(s) 307
MaeII ACGT 4 cut(s) 195, 212, 227, 733
MaeIII GTNAC 2 cut(s) 239, 674
MalI GATC 4 cut(s) 381, 543, 858, 977
MbiI CCGCTC 1 cut(s) 237
MboI GATC 4 cut(s) 379, 541, 856, 975
MfeI CAATTG 1 cut(s) 618
MhlI GDGCHC 3 cut(s) 98, 218, 263
MlsI TGGCCA 2 cut(s) 17, 329
MluCI AATT 4 cut(s) 167, 535, 575, 618
MluNI TGGCCA 2 cut(s) 17, 329
MlyI GAGTC 1 cut(s) 905
MmeI TCCRAC 2 cut(s) 59, 336
MnlI CCTC 8 cut(s) 85, 138, 270, 285, 316, 381, 559, 785
Mox20I TGGCCA 2 cut(s) 17, 329
MroNI GCCGGC 1 cut(s) 295
MscI TGGCCA 2 cut(s) 17, 329
Msp20I TGGCCA 2 cut(s) 17, 329
MspA1I CMGCKG 3 cut(s) 346, 486, 801
MspI CCGG 8 cut(s) 66, 173, 179, 296, 339, 491, 743, 877
MspR9I CCNGG 2 cut(s) 66, 264
MunI CAATTG 1 cut(s) 618
Mva1269I GAATGC 2 cut(s) 634, 893
MvaI CCWGG 1 cut(s) 264
MvnI CGCG 2 cut(s) 119, 476
MwoI GCNNNNNNNGC 6 cut(s) 102, 133, 254, 305, 326, 335
NaeI GCCGGC 1 cut(s) 297
NciI CCSGG 1 cut(s) 66
NdeII GATC 4 cut(s) 379, 541, 856, 975
NgoMIV GCCGGC 1 cut(s) 295
NlaIII CATG 1 cut(s) 873
NlaIV GGNNCC 5 cut(s) 64, 96, 177, 420, 667
NmeAIII GCCGAG 2 cut(s) 174, 346
NspI RCATGY 1 cut(s) 873
PciSI GCTCTTC 1 cut(s) 223
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PctI GAATGC 2 cut(s) 634, 893
PdiI GCCGGC 1 cut(s) 297
PfeI GAWTC 1 cut(s) 720
PinAI ACCGGT 2 cut(s) 178, 876
PkrI GCNGC 3 cut(s) 129, 132, 135
PleI GAGTC 1 cut(s) 905
PmaCI CACGTG 1 cut(s) 213
PmlI CACGTG 1 cut(s) 213
PpsI GAGTC 1 cut(s) 905
Ppu21I YACGTR 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 262
PspCI CACGTG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 5 cut(s) 64, 96, 177, 420, 667
PspOMI GGGCCC 1 cut(s) 94
PspPI GGNCC 5 cut(s) 62, 94, 95, 341, 809
PvuII CAGCTG 1 cut(s) 486
RsaI GTAC 2 cut(s) 57, 162
RsaNI GTAC 2 cut(s) 56, 161
Rsr2I CGGWCCG 1 cut(s) 341
RsrII CGGWCCG 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 223
SatI GCNGC 3 cut(s) 128, 131, 134
Sau3AI GATC 4 cut(s) 379, 541, 856, 975
Sau96I GGNCC 5 cut(s) 62, 94, 95, 341, 809
SchI GAGTC 1 cut(s) 905
ScrFI CCNGG 2 cut(s) 66, 264
SduI GDGCHC 3 cut(s) 98, 218, 263
SfaNI GCATC 1 cut(s) 307
SinI GGWCC 2 cut(s) 62, 341
SmlI CTYRAG 2 cut(s) 289, 644
SmoI CTYRAG 2 cut(s) 289, 644
Sse9I AATT 4 cut(s) 167, 535, 575, 618
StyD4I CCNGG 2 cut(s) 64, 262
TaaI ACNGT 2 cut(s) 354, 817
TaiI ACGT 4 cut(s) 198, 215, 230, 736
TaqI TCGA 1 cut(s) 233
TaqII GACCGA 1 cut(s) 50
TasI AATT 4 cut(s) 167, 535, 575, 618
TauI GCSGC 2 cut(s) 130, 133
TfiI GAWTC 1 cut(s) 720
TscAI CASTG 1 cut(s) 429
TseI GCWGC 1 cut(s) 133
TspDTI ATGAA 2 cut(s) 800, 986
TspGWI ACGGA 2 cut(s) 534, 713
TspRI CASTG 1 cut(s) 429
VpaK11BI GGWCC 2 cut(s) 62, 341
XceI RCATGY 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.