Rw6G035860

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0012176
Physical Location & Seq
Reverse (-)
60440310 .. 60442553
2244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035860.1

Sequence Viewer

Length: 945 bp
ATGGCAATGTCTGTGGCCACTGAGCTCCAATCTCCAATCTGCGAGCCTCGCTCCGTTCTCGGCCCAGGTGGGAACAGAGTTAGGGTTTCGGAGGGACCTAAACACAAATACGAGGGACTCAAGAAGCCACCGCAGAGGCCGAGAAAAGAAATTCCGGAATCGGTTGTCCGGAAAAACGTTTCGGTGGACAGCTCGTGCTCTTCAGACACCTCTTCCACTGGCTCCTCTCCCAAAACGGCCAGTCCTAGAAGGACTGTAAGGCATAAGAGCTTGAGGCCTGCCAAGCTTGTTTCAAACGACGTCGTCAAGCCTGACGGTCCACCTAAGCGATGTGAATGGATTACAGCGTACTCCGACCCACATTATATTTCTTTCCACGATGAAGAATGGGGAGTTCCAGTATATGATGATAAGAAGCTGTTTGAACTACTCGTCCTTTCGCAATCTCTAGCAGAACTCAGTTGGCCTGAAATTCTTACCAAGAGGGACATATTCAGGAATCTTTTTGACAACTTTGATCCTTCATCCATTGCAAAGTTTGATGAGAAGAAGTTACTGTCACTCAAAGTAAATGGCATCCCAATTCTATCCCAACAAAAGCTTCGTGCAATCGTTGACAATGCTATACAAGTGGTCAAGATTCAGCAAGAGGTTGGTTCCTTCAGTAAATATTGCTGGAGCTTTGTGAACCAGAAGGCGATCAACAATGGAGTTCGTTATGGACGTCAAGTACCAGTAAAGTCACCAAAAGCTGAAGTTATAAGCAAGGATTTGATGAAGAGAGGCTTCCGTTGTGTTGGTCCTACTGTTGTCTATTCATTCATGCAAGTAGCTGGGATTGTGAACGATCATCTTGTAACATGCTTCAGGCACAAAGTGTGCGATGTCAACGCCAAGTTCGATCCGAAACTTGATATCGAGAAGTCCAAGGATCTACCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

314

Amino Acids

35.31

Weight (kDa)

9.51

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 117 - 291 3e-67 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15970 AT1G15970 AT1G75090 AT1G80850
fragaria_vesca FvH4_2g40160
malus_domestica MD08G1035300.v1.1
prunus_persica Prupe.1G382800_v2.0.a1
pyrus_communis pycom08g02790
rosa_chinensis RchiOBHm_Chr6g0300571
rosa_laevigata RLG00000011323
rosa_multiflora Rmu_sc0021182.1_g000001 Rmu_sc0026329.1_g000001
rosa_roxburghii Rroxscaffold_7G00167660
rosa_rugosa Rorug06G0297300
rosa_samantha Rh6AG409800 Rh6BG416900 Rh6CG424800 Rh6DG410300
rosa_wichuraiana Rw6G035860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 761
AatII GACGTC 2 cut(s) 303, 727
AccIII TCCGGA 2 cut(s) 154, 168
AciI CCGC 1 cut(s) 131
AclI AACGTT 1 cut(s) 177
AclWI GGATC 3 cut(s) 512, 896, 939
AcoI YGGCCR 2 cut(s) 15, 237
AcsI RAATTY 2 cut(s) 150, 471
AcuI CTGAAG 4 cut(s) 186, 646, 774, 850
AcyI GRCGYC 2 cut(s) 300, 724
AdeI CACNNNGTG 1 cut(s) 877
AfaI GTAC 2 cut(s) 350, 732
AgsI TTSAA 2 cut(s) 294, 425
AjnI CCWGG 1 cut(s) 64
AjuI GAANNNNNNNTTGG 2 cut(s) 585, 617
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 9 cut(s) 25, 192, 270, 286, 418, 601, 681, 752, 833
AluI AGCT 9 cut(s) 25, 192, 270, 286, 418, 601, 681, 752, 833
Alw21I GWGCWC 2 cut(s) 27, 200
AlwI GGATC 3 cut(s) 512, 896, 939
Aor13HI TCCGGA 2 cut(s) 154, 168
AoxI GGCC 6 cut(s) 15, 61, 137, 237, 275, 464
ApoI RAATTY 2 cut(s) 150, 471
AspS9I GGNCC 4 cut(s) 62, 95, 317, 800
AsuHPI GGTGA 1 cut(s) 735
AvaII GGWCC 3 cut(s) 95, 317, 800
BalI TGGCCA 1 cut(s) 17
BanII GRGCYC 1 cut(s) 27
BauI CACGAG 1 cut(s) 193
Bbv12I GWGCWC 2 cut(s) 27, 200
BceAI ACGGC 1 cut(s) 252
BciT130I CCWGG 1 cut(s) 66
BfaI CTAG 2 cut(s) 246, 449
Bme1390I CCNGG 1 cut(s) 66
Bme18I GGWCC 3 cut(s) 95, 317, 800
BmgT120I GGNCC 4 cut(s) 62, 95, 317, 800
BmiI GGNNCC 3 cut(s) 96, 223, 658
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 1 cut(s) 585
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpmI CTGGAG 1 cut(s) 697
Bpu10I CCTNAGC 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 104, 292
BsaHI GRCGYC 2 cut(s) 300, 724
BsaJI CCNNGG 2 cut(s) 64, 927
BsaWI WCCGGW 2 cut(s) 154, 168
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bse1I ACTGG 4 cut(s) 223, 240, 398, 734
Bse3DI GCAATG 2 cut(s) 12, 528
BseAI TCCGGA 2 cut(s) 154, 168
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 64, 927
BseGI GGATG 2 cut(s) 524, 576
BseMI GCAATG 2 cut(s) 12, 528
BseMII CTCAG 2 cut(s) 12, 472
BseNI ACTGG 4 cut(s) 223, 240, 398, 734
BseRI GAGGAG 1 cut(s) 214
BseYI CCCAGC 1 cut(s) 833
BshFI GGCC 6 cut(s) 17, 63, 139, 239, 277, 466
BsiHKAI GWGCWC 2 cut(s) 27, 200
BsiSI CCGG 2 cut(s) 155, 169
BslFI GGGAC 3 cut(s) 108, 129, 500
BsmFI GGGAC 3 cut(s) 108, 129, 500
BsnI GGCC 6 cut(s) 17, 63, 139, 239, 277, 466
Bsp1286I GDGCHC 2 cut(s) 27, 200
Bsp13I TCCGGA 2 cut(s) 154, 168
Bsp143I GATC 5 cut(s) 517, 699, 847, 901, 931
BspACI CCGC 1 cut(s) 131
BspANI GGCC 6 cut(s) 17, 63, 139, 239, 277, 466
BspCNI CTCAG 2 cut(s) 13, 471
BspEI TCCGGA 2 cut(s) 154, 168
BspLI GGNNCC 3 cut(s) 96, 223, 658
BspPI GGATC 3 cut(s) 512, 896, 939
BspQI GCTCTTC 1 cut(s) 205
BsrDI GCAATG 2 cut(s) 12, 528
BsrI ACTGG 4 cut(s) 223, 240, 398, 734
BssECI CCNNGG 2 cut(s) 64, 927
BssMI GATC 5 cut(s) 517, 699, 847, 901, 931
BssNI GRCGYC 2 cut(s) 300, 724
BssSI CACGAG 1 cut(s) 193
BssT1I CCWWGG 1 cut(s) 927
Bst2BI CACGAG 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 4 cut(s) 256, 317, 558, 808
Bst6I CTCTTC 3 cut(s) 205, 217, 773
BstACI GRCGYC 2 cut(s) 300, 724
BstC8I GCNNGC 2 cut(s) 44, 279
BstDEI CTNAG 3 cut(s) 21, 324, 458
BstF5I GGATG 2 cut(s) 524, 576
BstKTI GATC 5 cut(s) 520, 702, 850, 904, 934
BstMBI GATC 5 cut(s) 517, 699, 847, 901, 931
BstMWI GCNNNNNNNGC 2 cut(s) 48, 283
BstNI CCWGG 1 cut(s) 66
BstNSI RCATGY 1 cut(s) 864
BstSCI CCNGG 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 931
BstYI RGATCY 1 cut(s) 931
BsuRI GGCC 6 cut(s) 17, 63, 139, 239, 277, 466
BtgZI GCGATG 2 cut(s) 343, 897
BtsCI GGATG 2 cut(s) 524, 576
BtsIMutI CAGTG 2 cut(s) 18, 216
Cac8I GCNNGC 2 cut(s) 44, 279
Cfr13I GGNCC 4 cut(s) 62, 95, 317, 800
Csp6I GTAC 2 cut(s) 349, 731
CspCI CAANNNNNGTGG 1 cut(s) 29
CviAII CATG 2 cut(s) 823, 861
CviQI GTAC 2 cut(s) 349, 731
DdeI CTNAG 3 cut(s) 21, 324, 458
DpnI GATC 5 cut(s) 519, 701, 849, 903, 933
DpnII GATC 5 cut(s) 517, 699, 847, 901, 931
DraIII CACNNNGTG 1 cut(s) 877
EaeI YGGCCR 2 cut(s) 15, 237
Eam1104I CTCTTC 3 cut(s) 205, 217, 773
EarI CTCTTC 3 cut(s) 205, 217, 773
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 927
Eco147I AGGCCT 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 27
Eco32I GATATC 1 cut(s) 916
Eco47I GGWCC 3 cut(s) 95, 317, 800
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 4 cut(s) 186, 646, 774, 850
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 64
EcoRV GATATC 1 cut(s) 916
EcoT14I CCWWGG 1 cut(s) 927
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 927
FaeI CATG 2 cut(s) 826, 864
FalI AAGNNNNNCTT 2 cut(s) 770, 802
FaqI GGGAC 3 cut(s) 108, 129, 500
FatI CATG 2 cut(s) 822, 860
FokI GGATG 2 cut(s) 511, 563
FriOI GRGCYC 1 cut(s) 27
FspBI CTAG 2 cut(s) 246, 449
GsaI CCCAGC 1 cut(s) 837
GsuI CTGGAG 1 cut(s) 697
HaeIII GGCC 6 cut(s) 17, 63, 139, 239, 277, 466
HapII CCGG 2 cut(s) 155, 169
Hin1I GRCGYC 2 cut(s) 300, 724
Hin1II CATG 2 cut(s) 826, 864
HincII GTYRAC 2 cut(s) 616, 889
HindII GTYRAC 2 cut(s) 616, 889
HindIII AAGCTT 2 cut(s) 284, 599
HinfI GANTC 4 cut(s) 117, 158, 499, 640
HpaII CCGG 2 cut(s) 155, 169
HphI GGTGA 1 cut(s) 735
Hpy166II GTNNAC 6 cut(s) 187, 320, 616, 688, 844, 889
Hpy188I TCNGA 4 cut(s) 91, 205, 355, 906
Hpy188III TCNNGA 6 cut(s) 121, 155, 169, 496, 637, 919
Hpy8I GTNNAC 6 cut(s) 187, 320, 616, 688, 844, 889
Hpy99I CGWCG 2 cut(s) 302, 305
HpyAV CCTTC 4 cut(s) 243, 531, 670, 688
HpyCH4III ACNGT 4 cut(s) 256, 317, 558, 808
HpyCH4IV ACGT 3 cut(s) 177, 300, 724
HpyCH4V TGCA 3 cut(s) 533, 608, 826
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 283
HpyF3I CTNAG 3 cut(s) 21, 324, 458
HpySE526I ACGT 3 cut(s) 177, 300, 724
Hsp92I GRCGYC 2 cut(s) 300, 724
Hsp92II CATG 2 cut(s) 826, 864
Kpn2I TCCGGA 2 cut(s) 154, 168
Kzo9I GATC 5 cut(s) 517, 699, 847, 901, 931
LguI GCTCTTC 1 cut(s) 205
LmnI GCTCC 4 cut(s) 30, 56, 227, 678
LweI GCATC 1 cut(s) 585
MaeI CTAG 2 cut(s) 246, 449
MaeII ACGT 3 cut(s) 177, 300, 724
MaeIII GTNAC 4 cut(s) 552, 558, 741, 856
MalI GATC 5 cut(s) 519, 701, 849, 903, 933
MboI GATC 5 cut(s) 517, 699, 847, 901, 931
MboII GAAGA 5 cut(s) 192, 204, 395, 559, 790
MflI RGATCY 1 cut(s) 931
MhlI GDGCHC 2 cut(s) 27, 200
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 3 cut(s) 150, 471, 582
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 1 cut(s) 111
MmeI TCCRAC 1 cut(s) 378
Mox20I TGGCCA 1 cut(s) 17
MroI TCCGGA 2 cut(s) 154, 168
MscI TGGCCA 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 17
MspI CCGG 2 cut(s) 155, 169
MspR9I CCNGG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 2 cut(s) 48, 283
NdeII GATC 5 cut(s) 517, 699, 847, 901, 931
NlaIII CATG 2 cut(s) 826, 864
NlaIV GGNNCC 3 cut(s) 96, 223, 658
NmeAIII GCCGAG 2 cut(s) 39, 165
NmuCI GTSAC 2 cut(s) 558, 741
NspI RCATGY 1 cut(s) 864
PceI AGGCCT 1 cut(s) 277
PciSI GCTCTTC 1 cut(s) 205
PcsI WCGNNNNNNNCGW 2 cut(s) 721, 897
PfeI GAWTC 3 cut(s) 158, 499, 640
PflFI GACNNNGTC 1 cut(s) 302
PleI GAGTC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 111
PpuMI RGGWCCY 1 cut(s) 95
PsiI TTATAA 1 cut(s) 761
Psp124BI GAGCTC 1 cut(s) 27
Psp1406I AACGTT 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 95
Psp6I CCWGG 1 cut(s) 64
PspFI CCCAGC 1 cut(s) 833
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 3 cut(s) 96, 223, 658
PspPI GGNCC 4 cut(s) 62, 95, 317, 800
PspPPI RGGWCCY 1 cut(s) 95
PsuI RGATCY 1 cut(s) 931
PsyI GACNNNGTC 1 cut(s) 302
RsaI GTAC 2 cut(s) 350, 732
RsaNI GTAC 2 cut(s) 349, 731
SacI GAGCTC 1 cut(s) 27
SapI GCTCTTC 1 cut(s) 205
Sau3AI GATC 5 cut(s) 517, 699, 847, 901, 931
Sau96I GGNCC 4 cut(s) 62, 95, 317, 800
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 2 cut(s) 27, 200
SfaNI GCATC 1 cut(s) 585
SinI GGWCC 3 cut(s) 95, 317, 800
SmlI CTYRAG 2 cut(s) 119, 271
SmoI CTYRAG 2 cut(s) 119, 271
Sse9I AATT 3 cut(s) 150, 471, 582
SseBI AGGCCT 1 cut(s) 277
SsiI CCGC 1 cut(s) 131
SspI AATATT 1 cut(s) 671
SspMI CTAG 2 cut(s) 246, 449
SstI GAGCTC 1 cut(s) 27
StuI AGGCCT 1 cut(s) 277
StyD4I CCNGG 1 cut(s) 64
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 4 cut(s) 256, 317, 558, 808
TaiI ACGT 3 cut(s) 180, 303, 727
TaqI TCGA 2 cut(s) 900, 918
TasI AATT 3 cut(s) 150, 471, 582
TfiI GAWTC 3 cut(s) 158, 499, 640
TscAI CASTG 2 cut(s) 25, 223
TseFI GTSAC 2 cut(s) 558, 741
Tsp45I GTSAC 2 cut(s) 558, 741
TspDTI ATGAA 5 cut(s) 396, 513, 791, 807, 811
TspGWI ACGGA 2 cut(s) 43, 779
TspRI CASTG 2 cut(s) 25, 223
Tth111I GACNNNGTC 1 cut(s) 302
VpaK11BI GGWCC 3 cut(s) 95, 317, 800
XapI RAATTY 2 cut(s) 150, 471
XceI RCATGY 1 cut(s) 864
XspI CTAG 2 cut(s) 246, 449
ZraI GACGTC 2 cut(s) 301, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.