Rh6BG416900

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0012176
Physical Location & Seq
Reverse (-)
64191743 .. 64197347
5605 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG416900.1

Sequence Viewer

Length: 741 bp
ATGGCAATGTCTCTGGCCACTGAGCTCCAATCTTCAATCTGCGAGCCTCGCGCCGTTCTCGGCCCCGGTGGGAACAGAGTTAGGGTTTCGGAGGGACCTAAACACAAATACGAGGGACTCAAGAAGCCATCGCAGAGGCCGAGAAAAGAAATTCCGGAATCGGTTGTCCGGAAAAACGTTTCGGTGGACAGCTCGTGCTCTTCAGACACCTCTTCCACTGGCTCCTCTCCCAAAACGGCCAGTCCTAGAAGGACTGTAAGGCATAAGAGCTTGAGGCCTGCCAAGCTTGTTTCAAACGACGTCGTCAAGCCTGACGGTCCACCTAAGCGATGTGAATGGATTACAGCTAACTCCGACCCACATTATATTTCTTTCCACGATGAAGAATGGGGAGTTCCAGTGTATGATGATAAGAAGCTGTTTGAACTACTCGTCCTTTCACAATCTCTAGCAGAACTCAGTTGGCCTGAAATTCTTACCAAGAGGGACATATTCAGGAATCTTTTTGACAACTTTGATCCTTCATCCATTGCAAAGTTTGATGAGAAGAAGTTACTGTCACTCAAAGTAAATGGCATCCCAATTCTATCCCAACAAAAGCTCCGTGCAATCGTCGACAATGCTATACAAGTGGTCAAGGTATTCAACATTGTTCGGGCAATGCAATCTCTAGATACTACATACGCTTGCATAACTTGTCCCTTTGAAAGGTTTTTCCCTGTTTCCAAAATCAACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

246

Amino Acids

27.61

Weight (kDa)

9.41

Isoelectric Point (pI)

56.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 116 - 216 1.6e-31 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15970 AT1G15970 AT1G75090 AT1G80850
fragaria_vesca FvH4_2g40160
malus_domestica MD08G1035300.v1.1
prunus_persica Prupe.1G382800_v2.0.a1
pyrus_communis pycom08g02790
rosa_chinensis RchiOBHm_Chr6g0300571
rosa_laevigata RLG00000011323
rosa_multiflora Rmu_sc0021182.1_g000001 Rmu_sc0026329.1_g000001
rosa_roxburghii Rroxscaffold_7G00167660
rosa_rugosa Rorug06G0297300
rosa_samantha Rh6AG409800 Rh6BG416900 Rh6CG424800 Rh6DG410300
rosa_wichuraiana Rw6G035860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 303
AccI GTMKAC 1 cut(s) 615
AccII CGCG 1 cut(s) 51
AccIII TCCGGA 2 cut(s) 154, 168
AclI AACGTT 1 cut(s) 177
AclWI GGATC 1 cut(s) 512
AcoI YGGCCR 2 cut(s) 15, 237
AcsI RAATTY 2 cut(s) 150, 471
AcuI CTGAAG 1 cut(s) 186
AcyI GRCGYC 1 cut(s) 300
AfiI CCNNNNNNNGG 1 cut(s) 708
AgsI TTSAA 5 cut(s) 36, 294, 425, 646, 707
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 7 cut(s) 25, 192, 270, 286, 347, 418, 601
AluI AGCT 7 cut(s) 25, 192, 270, 286, 347, 418, 601
Alw21I GWGCWC 2 cut(s) 27, 200
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 1 cut(s) 512
Aor13HI TCCGGA 2 cut(s) 154, 168
AoxI GGCC 6 cut(s) 15, 61, 137, 237, 275, 464
ApoI RAATTY 2 cut(s) 150, 471
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 3 cut(s) 62, 95, 317
AsuC2I CCSGG 1 cut(s) 66
AvaII GGWCC 2 cut(s) 95, 317
BalI TGGCCA 1 cut(s) 17
BanII GRGCYC 1 cut(s) 27
BauI CACGAG 1 cut(s) 193
Bbv12I GWGCWC 2 cut(s) 27, 200
BccI CCATC 1 cut(s) 136
BceAI ACGGC 2 cut(s) 38, 252
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 3 cut(s) 246, 449, 671
Bme1390I CCNGG 1 cut(s) 66
Bme18I GGWCC 2 cut(s) 95, 317
BmgT120I GGNCC 3 cut(s) 62, 95, 317
BmiI GGNNCC 3 cut(s) 64, 96, 223
BmrFI CCNGG 1 cut(s) 66
BmsI GCATC 1 cut(s) 585
Bpu10I CCTNAGC 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 104, 292
BpuMI CCSGG 1 cut(s) 66
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 64
BsaWI WCCGGW 2 cut(s) 154, 168
BsaXI ACNNNNNCTCC 4 cut(s) 384, 414, 585, 615
Bsc4I CCNNNNNNNGG 1 cut(s) 708
Bse1I ACTGG 3 cut(s) 223, 240, 398
Bse3DI GCAATG 3 cut(s) 12, 528, 666
BseAI TCCGGA 2 cut(s) 154, 168
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 2 cut(s) 524, 576
BseLI CCNNNNNNNGG 1 cut(s) 708
BseMI GCAATG 3 cut(s) 12, 528, 666
BseMII CTCAG 2 cut(s) 12, 472
BseNI ACTGG 3 cut(s) 223, 240, 398
BseRI GAGGAG 1 cut(s) 214
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 6 cut(s) 17, 63, 139, 239, 277, 466
BsiHKAI GWGCWC 2 cut(s) 27, 200
BsiSI CCGG 3 cut(s) 66, 155, 169
BslFI GGGAC 4 cut(s) 108, 129, 500, 684
BslI CCNNNNNNNGG 1 cut(s) 708
BsmAI GTCTC 1 cut(s) 15
BsmFI GGGAC 4 cut(s) 108, 129, 500, 684
BsnI GGCC 6 cut(s) 17, 63, 139, 239, 277, 466
Bsp1286I GDGCHC 2 cut(s) 27, 200
Bsp13I TCCGGA 2 cut(s) 154, 168
Bsp143I GATC 1 cut(s) 517
BspANI GGCC 6 cut(s) 17, 63, 139, 239, 277, 466
BspCNI CTCAG 2 cut(s) 13, 471
BspEI TCCGGA 2 cut(s) 154, 168
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 3 cut(s) 64, 96, 223
BspPI GGATC 1 cut(s) 512
BspQI GCTCTTC 1 cut(s) 205
BsrDI GCAATG 3 cut(s) 12, 528, 666
BsrI ACTGG 3 cut(s) 223, 240, 398
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 1 cut(s) 517
BssNI GRCGYC 1 cut(s) 300
BssSI CACGAG 1 cut(s) 193
Bst2BI CACGAG 1 cut(s) 193
Bst4CI ACNGT 3 cut(s) 256, 317, 558
Bst6I CTCTTC 2 cut(s) 205, 217
BstACI GRCGYC 1 cut(s) 300
BstC8I GCNNGC 3 cut(s) 44, 279, 688
BstDEI CTNAG 3 cut(s) 21, 324, 458
BstENI CCTNNNNNAGG 1 cut(s) 706
BstF5I GGATG 2 cut(s) 524, 576
BstFNI CGCG 1 cut(s) 51
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 1 cut(s) 520
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 1 cut(s) 517
BstMWI GCNNNNNNNGC 2 cut(s) 48, 283
BstSCI CCNGG 1 cut(s) 64
BstUI CGCG 1 cut(s) 51
BsuRI GGCC 6 cut(s) 17, 63, 139, 239, 277, 466
BtgZI GCGATG 2 cut(s) 114, 343
BtsCI GGATG 2 cut(s) 524, 576
BtsIMutI CAGTG 3 cut(s) 18, 216, 405
Cac8I GCNNGC 3 cut(s) 44, 279, 688
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 3 cut(s) 62, 95, 317
DdeI CTNAG 3 cut(s) 21, 324, 458
DpnI GATC 1 cut(s) 519
DpnII GATC 1 cut(s) 517
EaeI YGGCCR 2 cut(s) 15, 237
Eam1104I CTCTTC 2 cut(s) 205, 217
EarI CTCTTC 2 cut(s) 205, 217
Ecl136II GAGCTC 1 cut(s) 25
Eco147I AGGCCT 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 95, 317
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 186
EcoICRI GAGCTC 1 cut(s) 25
EcoNI CCTNNNNNAGG 1 cut(s) 706
EcoO109I RGGNCCY 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 27
FaiI YATR 7 cut(s) 264, 366, 405, 491, 626, 682, 692
FaqI GGGAC 4 cut(s) 108, 129, 500, 684
FblI GTMKAC 1 cut(s) 615
FokI GGATG 2 cut(s) 511, 563
FriOI GRGCYC 1 cut(s) 27
FspBI CTAG 3 cut(s) 246, 449, 671
GlaI GCGC 1 cut(s) 52
HaeIII GGCC 6 cut(s) 17, 63, 139, 239, 277, 466
HapII CCGG 3 cut(s) 66, 155, 169
HhaI GCGC 1 cut(s) 53
Hin1I GRCGYC 1 cut(s) 300
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HincII GTYRAC 1 cut(s) 616
HindII GTYRAC 1 cut(s) 616
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 3 cut(s) 117, 158, 499
HpaII CCGG 3 cut(s) 66, 155, 169
Hpy166II GTNNAC 3 cut(s) 187, 320, 616
Hpy188I TCNGA 3 cut(s) 91, 205, 355
Hpy188III TCNNGA 5 cut(s) 121, 155, 169, 496, 671
Hpy8I GTNNAC 3 cut(s) 187, 320, 616
Hpy99I CGWCG 3 cut(s) 302, 305, 617
HpyAV CCTTC 2 cut(s) 243, 531
HpyCH4III ACNGT 3 cut(s) 256, 317, 558
HpyCH4IV ACGT 2 cut(s) 177, 300
HpyCH4V TGCA 4 cut(s) 533, 608, 664, 690
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 283
HpyF3I CTNAG 3 cut(s) 21, 324, 458
HpySE526I ACGT 2 cut(s) 177, 300
Hsp92I GRCGYC 1 cut(s) 300
HspAI GCGC 1 cut(s) 51
Kpn2I TCCGGA 2 cut(s) 154, 168
Kzo9I GATC 1 cut(s) 517
LguI GCTCTTC 1 cut(s) 205
LmnI GCTCC 3 cut(s) 30, 227, 606
LweI GCATC 1 cut(s) 585
MaeI CTAG 3 cut(s) 246, 449, 671
MaeII ACGT 2 cut(s) 177, 300
MaeIII GTNAC 2 cut(s) 552, 558
MalI GATC 1 cut(s) 519
MboI GATC 1 cut(s) 517
MboII GAAGA 5 cut(s) 24, 192, 204, 395, 559
MhlI GDGCHC 2 cut(s) 27, 200
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 3 cut(s) 150, 471, 582
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 1 cut(s) 111
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 8 cut(s) 57, 85, 106, 129, 220, 235, 267, 477
Mox20I TGGCCA 1 cut(s) 17
MroI TCCGGA 2 cut(s) 154, 168
MscI TGGCCA 1 cut(s) 17
Msp20I TGGCCA 1 cut(s) 17
MspI CCGG 3 cut(s) 66, 155, 169
MspR9I CCNGG 1 cut(s) 66
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 2 cut(s) 48, 283
NciI CCSGG 1 cut(s) 66
NdeII GATC 1 cut(s) 517
NlaIV GGNNCC 3 cut(s) 64, 96, 223
NmeAIII GCCGAG 2 cut(s) 39, 165
NmuCI GTSAC 1 cut(s) 558
PceI AGGCCT 1 cut(s) 277
PciSI GCTCTTC 1 cut(s) 205
PcsI WCGNNNNNNNCGW 1 cut(s) 137
PfeI GAWTC 2 cut(s) 158, 499
PflFI GACNNNGTC 1 cut(s) 302
PleI GAGTC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 111
PpuMI RGGWCCY 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 27
Psp1406I AACGTT 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 95
PspN4I GGNNCC 3 cut(s) 64, 96, 223
PspPI GGNCC 3 cut(s) 62, 95, 317
PspPPI RGGWCCY 1 cut(s) 95
PsyI GACNNNGTC 1 cut(s) 302
SacI GAGCTC 1 cut(s) 27
SalI GTCGAC 1 cut(s) 614
SapI GCTCTTC 1 cut(s) 205
Sau3AI GATC 1 cut(s) 517
Sau96I GGNCC 3 cut(s) 62, 95, 317
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 2 cut(s) 27, 200
SfaNI GCATC 1 cut(s) 585
SinI GGWCC 2 cut(s) 95, 317
SmlI CTYRAG 2 cut(s) 119, 271
SmoI CTYRAG 2 cut(s) 119, 271
Sse9I AATT 3 cut(s) 150, 471, 582
SseBI AGGCCT 1 cut(s) 277
SspMI CTAG 3 cut(s) 246, 449, 671
SstI GAGCTC 1 cut(s) 27
StuI AGGCCT 1 cut(s) 277
StyD4I CCNGG 1 cut(s) 64
TaaI ACNGT 3 cut(s) 256, 317, 558
TaiI ACGT 2 cut(s) 180, 303
TaqI TCGA 1 cut(s) 615
TasI AATT 3 cut(s) 150, 471, 582
TfiI GAWTC 2 cut(s) 158, 499
TscAI CASTG 3 cut(s) 25, 223, 405
TseFI GTSAC 1 cut(s) 558
Tsp45I GTSAC 1 cut(s) 558
TspDTI ATGAA 2 cut(s) 396, 513
TspGWI ACGGA 1 cut(s) 593
TspRI CASTG 3 cut(s) 25, 223, 405
Tth111I GACNNNGTC 1 cut(s) 302
VpaK11BI GGWCC 2 cut(s) 95, 317
XagI CCTNNNNNAGG 1 cut(s) 706
XapI RAATTY 2 cut(s) 150, 471
XbaI TCTAGA 1 cut(s) 670
XmiI GTMKAC 1 cut(s) 615
XspI CTAG 3 cut(s) 246, 449, 671
ZraI GACGTC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.