MD08G1035300.v1.1

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0012176
Physical Location & Seq
Forward (+)
2464826 .. 2467130
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1035300.v1.1.491

Sequence Viewer

Length: 993 bp
ATGGCAATGTCGGTGGCCAGTAAGCTTCAATCTCCGATTTGCGAGCCGCGAGCCGTGCTCGGTCCCGGCGGGAACAGAGTTAGGGTTTTGGAGGGCCCGAAACGCAAAGACGACGGCGCGAAGAAGCCGCCGCAGCAGACACCGAGGCCGAAAAAATCTGTACCGGAATTGCCGGAACCAGTGGTGCGGAGCGACGTGTCGGTGGACAGCACGTGCTCTTCGGACACGTCGTCGAGCGGTTACTCTGCGAAGACGGCGAGTCCTGGAAAGATTGTGAGGCGTAAGAGCTTGAGGCCGGCGAGGGTTGCTTCCGACGATGCCGAGGTGGCCAAGCCCGCCGGTCCGCCGAAACGGTGCGAGTGGATTACACCTCACGCTGACCCGATTTATACTTGTTTCCACGACGAAGAATGGGGGGTTCCGGTGTATGACGATAAGAGGCTGTTTGAACTGCTTGTCCTTTCACAAGCTCTAGCGGAACTCAGCTGGCCGGAAATACTGCACAAGAGAGACATATTCAGGAAACTTTTCGACAATTTTGATCCGTCCTCCATTGCGAAGTTTGAAGAGAAGAAACTACTGTCTCTCAAGGTGCTGTCCGAACAAAAGCTTCGTGCAATTGAAGAGAATGCCAAACAAATGCTCAAGGTTCAGCAGGAGTTTGGTTCCTTCAGTAACTACTGCTGGAGCTTTGTGAACCACAAGCCCGTAAGAAATGGATTCCGTTATGCACGTCAAGTGCCGGTGAAGTCCCCAAAAGCTGAAGCCATAAGTAAAGATTTGATGAAGAGAGGCTTCCGCTGCGTCGGGCCTACTGTCGTTTACTCATTTATGCAAGTGGCGGGGATTGTGAACGATCATCTCGCAACATGCTTCAGGTTCAAGGAATGCAATGCAAATGCCAACGCAGACTCAAAACATAAGATTGAGAAGAAGACAGGAGAAGATGGCACTAAACTAACCGAAGCTACGAAGATCGGAGACCAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

331

Amino Acids

36.86

Weight (kDa)

9.4

Isoelectric Point (pI)

49.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 126 - 293 3.2e-66 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15970 AT1G15970 AT1G75090 AT1G80850
fragaria_vesca FvH4_2g40160
malus_domestica MD08G1035300.v1.1
prunus_persica Prupe.1G382800_v2.0.a1
pyrus_communis pycom08g02790
rosa_chinensis RchiOBHm_Chr6g0300571
rosa_laevigata RLG00000011323
rosa_multiflora Rmu_sc0021182.1_g000001 Rmu_sc0026329.1_g000001
rosa_roxburghii Rroxscaffold_7G00167660
rosa_rugosa Rorug06G0297300
rosa_samantha Rh6AG409800 Rh6BG416900 Rh6CG424800 Rh6DG410300
rosa_wichuraiana Rw6G035860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 237
AccII CGCG 2 cut(s) 49, 119
AclWI GGATC 1 cut(s) 536
AcoI YGGCCR 3 cut(s) 15, 327, 488
AcuI CTGAAG 3 cut(s) 655, 783, 859
AcvI CACGTG 1 cut(s) 213
AfaI GTAC 1 cut(s) 162
AflIII ACRYGT 2 cut(s) 195, 225
AgsI TTSAA 5 cut(s) 29, 449, 566, 623, 883
AhdI GACNNNNNGTC 2 cut(s) 229, 258
AjiI CACGTC 3 cut(s) 196, 228, 734
AjnI CCWGG 1 cut(s) 262
AloI GAACNNNNNNTCC 2 cut(s) 441, 473
AluBI AGCT 8 cut(s) 25, 288, 470, 486, 610, 690, 761, 968
AluI AGCT 8 cut(s) 25, 288, 470, 486, 610, 690, 761, 968
Alw21I GWGCWC 2 cut(s) 60, 218
Alw26I GTCTC 3 cut(s) 504, 588, 975
AlwI GGATC 1 cut(s) 536
AoxI GGCC 7 cut(s) 15, 94, 146, 293, 327, 488, 809
ApaI GGGCCC 1 cut(s) 98
ApeKI GCWGC 2 cut(s) 133, 801
Asp700I GAANNNNTTC 1 cut(s) 527
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 5 cut(s) 62, 94, 95, 341, 809
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 1 cut(s) 757
AvaII GGWCC 2 cut(s) 62, 341
BaeGI GKGCMC 1 cut(s) 98
BalI TGGCCA 2 cut(s) 17, 329
BanII GRGCYC 1 cut(s) 98
BbrPI CACGTG 1 cut(s) 213
BbsI GAAGAC 2 cut(s) 257, 941
Bbv12I GWGCWC 2 cut(s) 60, 218
BbvI GCAGC 2 cut(s) 145, 788
BccI CCATC 1 cut(s) 941
BceAI ACGGC 3 cut(s) 38, 130, 270
BciT130I CCWGG 1 cut(s) 264
BcnI CCSGG 1 cut(s) 66
BcoDI GTCTC 3 cut(s) 504, 588, 975
BfaI CTAG 1 cut(s) 473
BglI GCCNNNNNGGC 1 cut(s) 326
BisI GCNGC 5 cut(s) 47, 128, 131, 134, 802
BlsI GCNGC 5 cut(s) 48, 129, 132, 135, 803
Bme1390I CCNGG 2 cut(s) 66, 264
Bme18I GGWCC 2 cut(s) 62, 341
BmeRI GACNNNNNGTC 2 cut(s) 229, 258
BmgBI CACGTC 3 cut(s) 196, 228, 734
BmgT120I GGNCC 5 cut(s) 62, 94, 95, 341, 809
BmiI GGNNCC 5 cut(s) 64, 96, 177, 420, 667
BmrFI CCNGG 2 cut(s) 66, 264
BmsI GCATC 1 cut(s) 307
BpiI GAAGAC 2 cut(s) 257, 941
BplI GAGNNNNNCTC 2 cut(s) 42, 74
BpmI CTGGAG 1 cut(s) 706
BpuEI CTTGAG 3 cut(s) 310, 572, 629
BpuMI CCSGG 1 cut(s) 66
BsaAI YACGTR 1 cut(s) 213
BsaI GGTCTC 1 cut(s) 975
BsaJI CCNNGG 2 cut(s) 143, 321
BsaWI WCCGGW 2 cut(s) 163, 421
Bse118I RCCGGY 3 cut(s) 295, 338, 742
Bse1I ACTGG 2 cut(s) 18, 179
Bse3DI GCAATG 3 cut(s) 12, 552, 898
BseBI CCWGG 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 143, 321
BseMI GCAATG 3 cut(s) 12, 552, 898
BseMII CTCAG 1 cut(s) 496
BseNI ACTGG 2 cut(s) 18, 179
BseSI GKGCMC 1 cut(s) 98
BseXI GCAGC 2 cut(s) 145, 788
BsgI GTGCAG 1 cut(s) 485
Bsh1236I CGCG 2 cut(s) 49, 119
BshFI GGCC 7 cut(s) 17, 96, 148, 295, 329, 490, 811
BsiHKAI GWGCWC 2 cut(s) 60, 218
BsiSI CCGG 8 cut(s) 66, 164, 173, 296, 339, 422, 491, 743
BslFI GGGAC 2 cut(s) 48, 736
BsmAI GTCTC 3 cut(s) 504, 588, 975
BsmFI GGGAC 2 cut(s) 48, 736
BsmI GAATGC 2 cut(s) 634, 893
BsnI GGCC 7 cut(s) 17, 96, 148, 295, 329, 490, 811
Bso31I GGTCTC 1 cut(s) 975
Bsp120I GGGCCC 1 cut(s) 94
Bsp1286I GDGCHC 3 cut(s) 60, 98, 218
Bsp143I GATC 3 cut(s) 541, 856, 975
BspANI GGCC 7 cut(s) 17, 96, 148, 295, 329, 490, 811
BspCNI CTCAG 1 cut(s) 495
BspFNI CGCG 2 cut(s) 49, 119
BspLI GGNNCC 5 cut(s) 64, 96, 177, 420, 667
BspPI GGATC 1 cut(s) 536
BspQI GCTCTTC 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 975
BsrBI CCGCTC 1 cut(s) 237
BsrDI GCAATG 3 cut(s) 12, 552, 898
BsrFI RCCGGY 3 cut(s) 295, 338, 742
BsrI ACTGG 2 cut(s) 18, 179
BssAI RCCGGY 3 cut(s) 295, 338, 742
BssECI CCNNGG 2 cut(s) 143, 321
BssMI GATC 3 cut(s) 541, 856, 975
Bst2UI CCWGG 1 cut(s) 264
Bst4CI ACNGT 3 cut(s) 354, 582, 817
Bst6I CTCTTC 4 cut(s) 223, 561, 618, 782
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 5 cut(s) 44, 51, 297, 336, 488
BstDEI CTNAG 1 cut(s) 482
BstFNI CGCG 2 cut(s) 49, 119
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 3 cut(s) 544, 859, 978
BstMAI GTCTC 3 cut(s) 504, 588, 975
BstMBI GATC 3 cut(s) 541, 856, 975
BstMWI GCNNNNNNNGC 8 cut(s) 55, 102, 133, 254, 305, 326, 335, 801
BstNI CCWGG 1 cut(s) 264
BstNSI RCATGY 1 cut(s) 873
BstSCI CCNGG 2 cut(s) 64, 262
BstSLI GKGCMC 1 cut(s) 98
BstUI CGCG 2 cut(s) 49, 119
BstV1I GCAGC 2 cut(s) 145, 788
BstV2I GAAGAC 2 cut(s) 257, 941
BsuRI GGCC 7 cut(s) 17, 96, 148, 295, 329, 490, 811
BtrI CACGTC 3 cut(s) 196, 228, 734
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 5 cut(s) 44, 51, 297, 336, 488
CfoI GCGC 1 cut(s) 119
Cfr10I RCCGGY 3 cut(s) 295, 338, 742
Cfr13I GGNCC 5 cut(s) 62, 94, 95, 341, 809
CpoI CGGWCCG 1 cut(s) 341
CseI GACGC 1 cut(s) 793
Csp6I GTAC 1 cut(s) 161
CspCI CAANNNNNGTGG 1 cut(s) 29
CspI CGGWCCG 1 cut(s) 341
CviAII CATG 1 cut(s) 870
CviQI GTAC 1 cut(s) 161
DdeI CTNAG 1 cut(s) 482
DpnI GATC 3 cut(s) 543, 858, 977
DpnII GATC 3 cut(s) 541, 856, 975
DriI GACNNNNNGTC 2 cut(s) 229, 258
EaeI YGGCCR 3 cut(s) 15, 327, 488
Eam1104I CTCTTC 4 cut(s) 223, 561, 618, 782
Eam1105I GACNNNNNGTC 2 cut(s) 229, 258
EarI CTCTTC 4 cut(s) 223, 561, 618, 782
EciI GGCGGA 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 98
Eco31I GGTCTC 1 cut(s) 975
Eco47I GGWCC 2 cut(s) 62, 341
Eco57I CTGAAG 3 cut(s) 655, 783, 859
Eco72I CACGTG 1 cut(s) 213
EcoO109I RGGNCCY 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 262
EcoT38I GRGCYC 1 cut(s) 98
FaeI CATG 1 cut(s) 873
FaiI YATR 8 cut(s) 390, 429, 515, 729, 770, 833, 871, 921
FalI AAGNNNNNCTT 2 cut(s) 779, 811
FaqI GGGAC 2 cut(s) 48, 736
FatI CATG 1 cut(s) 869
FauI CCCGC 3 cut(s) 62, 343, 835
Fnu4HI GCNGC 5 cut(s) 47, 128, 131, 134, 802
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 5 cut(s) 47, 128, 131, 134, 802
FspBI CTAG 1 cut(s) 473
GlaI GCGC 1 cut(s) 118
GluI GCNGC 5 cut(s) 47, 128, 131, 134, 802
GsuI CTGGAG 1 cut(s) 706
HaeIII GGCC 7 cut(s) 17, 96, 148, 295, 329, 490, 811
HapII CCGG 8 cut(s) 66, 164, 173, 296, 339, 422, 491, 743
HgaI GACGC 1 cut(s) 793
HhaI GCGC 1 cut(s) 119
Hin1II CATG 1 cut(s) 873
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HindIII AAGCTT 2 cut(s) 23, 608
HinfI GANTC 3 cut(s) 259, 720, 911
HpaII CCGG 8 cut(s) 66, 164, 173, 296, 339, 422, 491, 743
HphI GGTGA 1 cut(s) 757
Hpy166II GTNNAC 4 cut(s) 205, 697, 823, 853
Hpy188I TCNGA 5 cut(s) 36, 223, 313, 601, 980
Hpy188III TCNNGA 1 cut(s) 520
Hpy8I GTNNAC 4 cut(s) 205, 697, 823, 853
Hpy99I CGWCG 7 cut(s) 116, 197, 232, 235, 317, 407, 809
HpyAV CCTTC 1 cut(s) 679
HpyCH4III ACNGT 3 cut(s) 354, 582, 817
HpyCH4IV ACGT 4 cut(s) 195, 212, 227, 733
HpyCH4V TGCA 6 cut(s) 502, 617, 731, 835, 891, 896
HpyF10VI GCNNNNNNNGC 8 cut(s) 55, 102, 133, 254, 305, 326, 335, 801
HpyF3I CTNAG 1 cut(s) 482
HpySE526I ACGT 4 cut(s) 195, 212, 227, 733
Hsp92II CATG 1 cut(s) 873
HspAI GCGC 1 cut(s) 117
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
Kzo9I GATC 3 cut(s) 541, 856, 975
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 2 cut(s) 189, 687
Lsp1109I GCAGC 2 cut(s) 145, 788
LweI GCATC 1 cut(s) 307
MaeI CTAG 1 cut(s) 473
MaeII ACGT 4 cut(s) 195, 212, 227, 733
MaeIII GTNAC 2 cut(s) 239, 674
MalI GATC 3 cut(s) 543, 858, 977
MbiI CCGCTC 1 cut(s) 237
MboI GATC 3 cut(s) 541, 856, 975
MfeI CAATTG 1 cut(s) 618
MhlI GDGCHC 3 cut(s) 60, 98, 218
MlsI TGGCCA 2 cut(s) 17, 329
MluCI AATT 3 cut(s) 167, 535, 618
MluNI TGGCCA 2 cut(s) 17, 329
MlyI GAGTC 2 cut(s) 268, 905
MmeI TCCRAC 1 cut(s) 336
Mox20I TGGCCA 2 cut(s) 17, 329
MroNI GCCGGC 1 cut(s) 295
MroXI GAANNNNTTC 1 cut(s) 527
MscI TGGCCA 2 cut(s) 17, 329
Msp20I TGGCCA 2 cut(s) 17, 329
MspA1I CMGCKG 2 cut(s) 486, 801
MspI CCGG 8 cut(s) 66, 164, 173, 296, 339, 422, 491, 743
MspR9I CCNGG 2 cut(s) 66, 264
MunI CAATTG 1 cut(s) 618
Mva1269I GAATGC 2 cut(s) 634, 893
MvaI CCWGG 1 cut(s) 264
MvnI CGCG 2 cut(s) 49, 119
MwoI GCNNNNNNNGC 8 cut(s) 55, 102, 133, 254, 305, 326, 335, 801
NaeI GCCGGC 1 cut(s) 297
NciI CCSGG 1 cut(s) 66
NdeII GATC 3 cut(s) 541, 856, 975
NgoMIV GCCGGC 1 cut(s) 295
NlaIII CATG 1 cut(s) 873
NlaIV GGNNCC 5 cut(s) 64, 96, 177, 420, 667
NmeAIII GCCGAG 1 cut(s) 346
NspI RCATGY 1 cut(s) 873
PciSI GCTCTTC 1 cut(s) 223
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PctI GAATGC 2 cut(s) 634, 893
PdiI GCCGGC 1 cut(s) 297
PdmI GAANNNNTTC 1 cut(s) 527
PfeI GAWTC 1 cut(s) 720
PfoI TCCNGGA 1 cut(s) 262
PkrI GCNGC 5 cut(s) 48, 129, 132, 135, 803
PleI GAGTC 2 cut(s) 267, 905
PmaCI CACGTG 1 cut(s) 213
PmlI CACGTG 1 cut(s) 213
PpsI GAGTC 2 cut(s) 267, 905
Ppu21I YACGTR 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 262
PspCI CACGTG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 262
PspN4I GGNNCC 5 cut(s) 64, 96, 177, 420, 667
PspOMI GGGCCC 1 cut(s) 94
PspPI GGNCC 5 cut(s) 62, 94, 95, 341, 809
PvuII CAGCTG 1 cut(s) 486
RsaI GTAC 1 cut(s) 162
RsaNI GTAC 1 cut(s) 161
Rsr2I CGGWCCG 1 cut(s) 341
RsrII CGGWCCG 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 223
SatI GCNGC 5 cut(s) 47, 128, 131, 134, 802
Sau3AI GATC 3 cut(s) 541, 856, 975
Sau96I GGNCC 5 cut(s) 62, 94, 95, 341, 809
SchI GAGTC 2 cut(s) 268, 905
ScrFI CCNGG 2 cut(s) 66, 264
SduI GDGCHC 3 cut(s) 60, 98, 218
SfaNI GCATC 1 cut(s) 307
SinI GGWCC 2 cut(s) 62, 341
SmlI CTYRAG 3 cut(s) 289, 587, 644
SmoI CTYRAG 3 cut(s) 289, 587, 644
Sse9I AATT 3 cut(s) 167, 535, 618
SspMI CTAG 1 cut(s) 473
StyD4I CCNGG 2 cut(s) 64, 262
TaaI ACNGT 3 cut(s) 354, 582, 817
TaiI ACGT 4 cut(s) 198, 215, 230, 736
TaqI TCGA 2 cut(s) 233, 531
TaqII GACCGA 1 cut(s) 50
TasI AATT 3 cut(s) 167, 535, 618
TauI GCSGC 3 cut(s) 49, 130, 133
TfiI GAWTC 1 cut(s) 720
TscAI CASTG 1 cut(s) 186
TseI GCWGC 2 cut(s) 133, 801
TspDTI ATGAA 1 cut(s) 800
TspGWI ACGGA 2 cut(s) 534, 713
TspRI CASTG 1 cut(s) 186
VpaK11BI GGWCC 2 cut(s) 62, 341
XceI RCATGY 1 cut(s) 873
XmnI GAANNNNTTC 1 cut(s) 527
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.