Rh6AG409800

Methyladenine glycosylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0012176
Physical Location & Seq
Reverse (-)
60654074 .. 60656444
2371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG409800.1

Sequence Viewer

Length: 666 bp
ATGGCAATGTCTGTGGCCACTGAGCTCCAATCTCCAATCTGCGAGCCTCGCGCCGTTCTCGGCCCAGGTGGGAACAGAGTTAGGGTTTCGGAGGGACCTAAACACAAATACGAGGGACTCAAGAAGCCACCACAGAGGCCGAGAAAAGAAATTCCGGAATCGGTTGTCCGGAAAAACGTTTCGGTGGACAGCTCGTGCTCTTCAGACACCTCTTCCACTGGCTCCTCTCCCAAAACTGCCAGTCCTAGAAGGACTGTAAGGCATAAGAGCTTGAGGCCTGCCAAGCTTGTTTCAAACGACGTCGTCAAGCCTGACGGTCCACCTAAGCGATGTGAATGGATTACAGCGTACTCCGACCCACATTATATTTCTTTCCACGACGAAGAATGGGGAGTTCCAGTATATGATGATAAGAAGCTGTTTGAACTACTCGTCCTTTCGCAATCTCTAGCAGAACTCAGTTGGCCTGAAATTCTTACCAAGAGGGACATATTCAGGTGTGCTTGTTTCCATTTCATCTATCATTTGAGTTTTGTGAAGAATGGTGCCCTTATGCTTAATGCTTTTTATGGTTGTTTCATGTCTTTGGCAGGAATCTTTTTGACAACTTTGATCCTTCATCCATTGCAAAGTTTGATGAGAAGAAGTTACTGTCACTCAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03410 map03410
Pfam Domains
Protein Families
Adenine_glyco

Protein Analysis

221

Amino Acids

24.89

Weight (kDa)

9.34

Isoelectric Point (pI)

64.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 117 - 172 5e-18 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012176)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15970 AT1G15970 AT1G75090 AT1G80850
fragaria_vesca FvH4_2g40160
malus_domestica MD08G1035300.v1.1
prunus_persica Prupe.1G382800_v2.0.a1
pyrus_communis pycom08g02790
rosa_chinensis RchiOBHm_Chr6g0300571
rosa_laevigata RLG00000011323
rosa_multiflora Rmu_sc0021182.1_g000001 Rmu_sc0026329.1_g000001
rosa_roxburghii Rroxscaffold_7G00167660
rosa_rugosa Rorug06G0297300
rosa_samantha Rh6AG409800 Rh6BG416900 Rh6CG424800 Rh6DG410300
rosa_wichuraiana Rw6G035860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 303
AccB1I GGYRCC 1 cut(s) 545
AccII CGCG 1 cut(s) 51
AccIII TCCGGA 2 cut(s) 154, 168
AclI AACGTT 1 cut(s) 177
AclWI GGATC 1 cut(s) 607
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 2 cut(s) 150, 471
AcuI CTGAAG 1 cut(s) 186
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 1 cut(s) 350
AgsI TTSAA 2 cut(s) 294, 425
AjnI CCWGG 1 cut(s) 64
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 5 cut(s) 25, 192, 270, 286, 418
AluI AGCT 5 cut(s) 25, 192, 270, 286, 418
Alw21I GWGCWC 2 cut(s) 27, 200
AlwI GGATC 1 cut(s) 607
Aor13HI TCCGGA 2 cut(s) 154, 168
AoxI GGCC 5 cut(s) 15, 61, 137, 275, 464
ApoI RAATTY 2 cut(s) 150, 471
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 3 cut(s) 62, 95, 317
AvaII GGWCC 2 cut(s) 95, 317
BaeGI GKGCMC 1 cut(s) 550
BalI TGGCCA 1 cut(s) 17
BanI GGYRCC 1 cut(s) 545
BanII GRGCYC 1 cut(s) 27
BauI CACGAG 1 cut(s) 193
Bbv12I GWGCWC 2 cut(s) 27, 200
BceAI ACGGC 1 cut(s) 38
BciT130I CCWGG 1 cut(s) 66
BfaI CTAG 2 cut(s) 246, 449
Bme1390I CCNGG 1 cut(s) 66
Bme18I GGWCC 2 cut(s) 95, 317
BmgT120I GGNCC 3 cut(s) 62, 95, 317
BmiI GGNNCC 3 cut(s) 96, 223, 547
BmrFI CCNGG 1 cut(s) 66
Bpu10I CCTNAGC 1 cut(s) 324
BpuEI CTTGAG 2 cut(s) 104, 292
BsaHI GRCGYC 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 64
BsaWI WCCGGW 2 cut(s) 154, 168
BsaXI ACNNNNNCTCC 2 cut(s) 384, 414
Bse1I ACTGG 3 cut(s) 223, 240, 398
Bse3DI GCAATG 2 cut(s) 12, 623
BseAI TCCGGA 2 cut(s) 154, 168
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 1 cut(s) 619
BseMI GCAATG 2 cut(s) 12, 623
BseMII CTCAG 2 cut(s) 12, 472
BseNI ACTGG 3 cut(s) 223, 240, 398
BseRI GAGGAG 1 cut(s) 214
BseSI GKGCMC 1 cut(s) 550
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 5 cut(s) 17, 63, 139, 277, 466
BshNI GGYRCC 1 cut(s) 545
BsiHKAI GWGCWC 2 cut(s) 27, 200
BsiSI CCGG 2 cut(s) 155, 169
BslFI GGGAC 3 cut(s) 108, 129, 500
BsmFI GGGAC 3 cut(s) 108, 129, 500
BsnI GGCC 5 cut(s) 17, 63, 139, 277, 466
Bsp1286I GDGCHC 3 cut(s) 27, 200, 550
Bsp13I TCCGGA 2 cut(s) 154, 168
Bsp143I GATC 1 cut(s) 612
BspANI GGCC 5 cut(s) 17, 63, 139, 277, 466
BspCNI CTCAG 2 cut(s) 13, 471
BspEI TCCGGA 2 cut(s) 154, 168
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 3 cut(s) 96, 223, 547
BspPI GGATC 1 cut(s) 607
BspQI GCTCTTC 1 cut(s) 205
BspT107I GGYRCC 1 cut(s) 545
BsrDI GCAATG 2 cut(s) 12, 623
BsrI ACTGG 3 cut(s) 223, 240, 398
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 1 cut(s) 612
BssNI GRCGYC 1 cut(s) 300
BssSI CACGAG 1 cut(s) 193
Bst2BI CACGAG 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 66
Bst4CI ACNGT 3 cut(s) 256, 317, 653
Bst6I CTCTTC 2 cut(s) 205, 217
BstACI GRCGYC 1 cut(s) 300
BstC8I GCNNGC 2 cut(s) 44, 279
BstDEI CTNAG 3 cut(s) 21, 324, 458
BstF5I GGATG 1 cut(s) 619
BstFNI CGCG 1 cut(s) 51
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 1 cut(s) 615
BstMBI GATC 1 cut(s) 612
BstMWI GCNNNNNNNGC 2 cut(s) 48, 283
BstNI CCWGG 1 cut(s) 66
BstSCI CCNGG 1 cut(s) 64
BstSLI GKGCMC 1 cut(s) 550
BstUI CGCG 1 cut(s) 51
BsuRI GGCC 5 cut(s) 17, 63, 139, 277, 466
BtgZI GCGATG 1 cut(s) 343
BtsCI GGATG 1 cut(s) 619
BtsIMutI CAGTG 2 cut(s) 18, 216
Cac8I GCNNGC 2 cut(s) 44, 279
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 3 cut(s) 62, 95, 317
Csp6I GTAC 1 cut(s) 349
CspCI CAANNNNNGTGG 1 cut(s) 29
CviAII CATG 1 cut(s) 580
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 3 cut(s) 21, 324, 458
DpnI GATC 1 cut(s) 614
DpnII GATC 1 cut(s) 612
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 2 cut(s) 205, 217
EarI CTCTTC 2 cut(s) 205, 217
Ecl136II GAGCTC 1 cut(s) 25
Eco147I AGGCCT 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 95, 317
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 186
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 64
EcoT38I GRGCYC 1 cut(s) 27
FaeI CATG 1 cut(s) 583
FaiI YATR 8 cut(s) 264, 366, 403, 405, 491, 554, 570, 581
FaqI GGGAC 3 cut(s) 108, 129, 500
FatI CATG 1 cut(s) 579
FokI GGATG 1 cut(s) 606
FriOI GRGCYC 1 cut(s) 27
FspBI CTAG 2 cut(s) 246, 449
GlaI GCGC 1 cut(s) 52
HaeIII GGCC 5 cut(s) 17, 63, 139, 277, 466
HapII CCGG 2 cut(s) 155, 169
HhaI GCGC 1 cut(s) 53
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 1 cut(s) 583
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 284
HinfI GANTC 3 cut(s) 117, 158, 594
HpaII CCGG 2 cut(s) 155, 169
Hpy166II GTNNAC 2 cut(s) 187, 320
Hpy188I TCNGA 3 cut(s) 91, 205, 355
Hpy188III TCNNGA 3 cut(s) 121, 155, 169
Hpy8I GTNNAC 2 cut(s) 187, 320
Hpy99I CGWCG 3 cut(s) 302, 305, 383
HpyAV CCTTC 2 cut(s) 243, 626
HpyCH4III ACNGT 3 cut(s) 256, 317, 653
HpyCH4IV ACGT 2 cut(s) 177, 300
HpyCH4V TGCA 1 cut(s) 628
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 283
HpyF3I CTNAG 3 cut(s) 21, 324, 458
HpySE526I ACGT 2 cut(s) 177, 300
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 1 cut(s) 583
HspAI GCGC 1 cut(s) 51
Kpn2I TCCGGA 2 cut(s) 154, 168
Kzo9I GATC 1 cut(s) 612
LguI GCTCTTC 1 cut(s) 205
LmnI GCTCC 2 cut(s) 30, 227
MaeI CTAG 2 cut(s) 246, 449
MaeII ACGT 2 cut(s) 177, 300
MaeIII GTNAC 2 cut(s) 647, 653
MalI GATC 1 cut(s) 614
MboI GATC 1 cut(s) 612
MboII GAAGA 5 cut(s) 192, 204, 395, 550, 654
MhlI GDGCHC 3 cut(s) 27, 200, 550
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 2 cut(s) 150, 471
MluNI TGGCCA 1 cut(s) 17
MlyI GAGTC 1 cut(s) 111
MmeI TCCRAC 1 cut(s) 378
MnlI CCTC 8 cut(s) 57, 85, 106, 129, 220, 235, 267, 477
Mox20I TGGCCA 1 cut(s) 17
MroI TCCGGA 2 cut(s) 154, 168
MscI TGGCCA 1 cut(s) 17
MseI TTAA 1 cut(s) 558
Msp20I TGGCCA 1 cut(s) 17
MspI CCGG 2 cut(s) 155, 169
MspR9I CCNGG 1 cut(s) 66
MvaI CCWGG 1 cut(s) 66
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 2 cut(s) 48, 283
NdeII GATC 1 cut(s) 612
NlaIII CATG 1 cut(s) 583
NlaIV GGNNCC 3 cut(s) 96, 223, 547
NmeAIII GCCGAG 2 cut(s) 39, 165
NmuCI GTSAC 1 cut(s) 653
PceI AGGCCT 1 cut(s) 277
PciSI GCTCTTC 1 cut(s) 205
PfeI GAWTC 2 cut(s) 158, 594
PflFI GACNNNGTC 1 cut(s) 302
PleI GAGTC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 111
PpuMI RGGWCCY 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 27
Psp1406I AACGTT 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 95
Psp6I CCWGG 1 cut(s) 64
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 3 cut(s) 96, 223, 547
PspPI GGNCC 3 cut(s) 62, 95, 317
PspPPI RGGWCCY 1 cut(s) 95
PsyI GACNNNGTC 1 cut(s) 302
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SacI GAGCTC 1 cut(s) 27
SapI GCTCTTC 1 cut(s) 205
SaqAI TTAA 1 cut(s) 558
Sau3AI GATC 1 cut(s) 612
Sau96I GGNCC 3 cut(s) 62, 95, 317
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 1 cut(s) 66
SduI GDGCHC 3 cut(s) 27, 200, 550
SinI GGWCC 2 cut(s) 95, 317
SmlI CTYRAG 2 cut(s) 119, 271
SmoI CTYRAG 2 cut(s) 119, 271
Sse9I AATT 2 cut(s) 150, 471
SseBI AGGCCT 1 cut(s) 277
SspMI CTAG 2 cut(s) 246, 449
SstI GAGCTC 1 cut(s) 27
StuI AGGCCT 1 cut(s) 277
StyD4I CCNGG 1 cut(s) 64
TaaI ACNGT 3 cut(s) 256, 317, 653
TaiI ACGT 2 cut(s) 180, 303
TasI AATT 2 cut(s) 150, 471
TfiI GAWTC 2 cut(s) 158, 594
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TscAI CASTG 2 cut(s) 25, 223
TseFI GTSAC 1 cut(s) 653
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 3 cut(s) 505, 568, 608
TspRI CASTG 2 cut(s) 25, 223
Tth111I GACNNNGTC 1 cut(s) 302
VpaK11BI GGWCC 2 cut(s) 95, 317
XapI RAATTY 2 cut(s) 150, 471
XspI CTAG 2 cut(s) 246, 449
ZraI GACGTC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.