FvH4_3g00230

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006300
Physical Location & Seq
Reverse (-)
129695 .. 135336
5642 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00230.t1

Sequence Viewer

Length: 3039 bp
ATGAAGCTGGTGGTGCAAGTGATGGGGGCCCGGGATCTTCCGGCAATGGATCTCAACGGCCTGAGTGATCCGTACGTGAAGGTGAAGCTGGGAAAGCAGAAGTTCAAGACCAAAGTGGTGAAGAAGACCTTAAACCCCTATTGGGGAGAAGAGTTTGCGTTCCGAGTCGACGACCTCAGCGACGAGCTCCTCATCTCTGTTCTGGACGAGGACAAGTACTTCAACGACGACTTCGTCGGCTGCGTCAAATTCCCCGTCTCCCAAGTCTTCGATTCCCACAACAAATGCCTCGATACTTGTTGGCACCCTCTCCAGCCCAAGAGCAAGAAGCCCAAGCACAAAGATTGCGGCGAGATTCTTCTTAATATATCTTTCATTACCAACAATGCCTTCTCCGATTCGGCTTCTGAGGGTGATCATTTTAGGAGGGACAGTGACGTCGGCGCTGAATCACCCTCGAGGTCTTTCGTATCCGAAACGGCTTCCCCTCAGAGAGGCAAGCTGGACGACAAGGAGGAAAAGGAAAAGAGTCTTGCCCAGAAATCCCTTGCTGGTCGACTTGTTCAGATGTTCAATAAAAATCCTGATGTACCTGCGATATCTTCAACCCATTCTAGCAAAACTGACTTGACTGAGCTTGTCGACATTGCTGAGGCCACTTCCGAGGATCACTCCGCTTCTGTTCCTTTTGATGAACTGATGAAGACAATGCAGTCACGAGAGCAGCCTGCTGAAACCCCTGAGAACCTACCAGGAGGAGTGCTTCTGGATCAAATGTATGTAACTGAACCCAAGCACCTCAACACTTTGATCTTTTCGCCAGATTCTACTTTTCCAAAGGCGCTAGCGGATGTGCATGGAACTACAGAGCTGGAACAAGGACCCTGGAAATTTGAAAATGACTGCTTGAAAAGAGTGGTTACTTATGTTAAGGCAGCTAGCAAATTAGTCAAGGCTTGCAAAGCAACCGAGGACCAACAATATCTCAAAGCCGACGGCAAGGTTTTTGCAGTTTTAGCTAGTGTAAGCACTCCTGATGTTCCATATGGGAAAACTTTTAGGACGGAGTTGCTCTTCTGCATCACACCTGGGCCTGAGCTGCCATCAGGAGAACAATGTACACGCTTTGTAATATCGTGGCGGATGAATTTCCTGCAGAGCACCATGATGAAAGGAATGATAGAAAATGGAGCTAGGCAAGGTTTAAAGGACAGTTATGAGCAGTATGCAACTTTATTATCGCAGAACGTTAAGCCAGCTGATTCAAAAGACCTTGGATCCAATAAGGACCAGGTTTTGGCATCACTGCAGGCTGAACCTCAGTCTGATTGGAAGCTGGCAGTACAATATTTTGCTAATTTCACTGTGGTCTCCACATTTTTCATAGGATTTTATATGATGGTACACATCTGGCTGGCTACACCCAGCACTATTCAAGGCCTGGAGTTTGTTGGGCTTGACTTGCCAGATTCACTTGGTGAATTCATTGTTTGTGGTGTCCTGGCACTTCAAGGAGAACGTGTGCTGGGTTTGATCTCCCGCTTTATGCAGGCCAGAGTGCAGAAAGGCAGTGATCATGGAGTCAAAGCTCGAGGAGATGGTTGGTTGCTAACTGTTGCCTTGATTGAAGGAAGTAATATAGCAGCTGTTGATTCAACCGGGTTCTCTGATCCATATGTGGTGTTTTCTTGCAATGGAAAAACTAGAACTAGCTCAATCAAGTTCCAAAAATGTGACCCAATGTGGAATGAAATATTTGAGTTTGATGCAATGGATGAGCCTCCTTCTGTGCTGGATGTGGAAATTTATGATTTTGATGGTCCTTTCGATGAAGCTACCTCTCTGGGCCATGCTGAAATCAATTTTGTGAAAACCAATATATCAGATTTAGCTGATTTGTGGATTCCTCTTCAAGGAAAGTTAGCTCAGGCTTGTCAGTCAAAACTACACTTGAGAATTTTCTTAAACAATACAAGAGGAGGCAATGTTGTTAATCACTTTATAAATAAGATGGAGAAGGAGGTCGGAAAGAAGATTACTGTACGTTCTCCACAAACTAATTCAGCATTCCAAAAACTCTTTGGCCTTCCACCAGAGGAATTTCTTATCAATGACTTCACATGTCACTTGAAACGAAAAATGCCCCTGCAGGGCCGCCTGTTTCTTTCTGCGAGAATAATTGGGTTCCATGCAAATTTATTTGGTCGCAAGACAAAGTTTTTTTTCCTCTGGGAGGATATAGAAGATATTCATATTGTTCCTCCAACTCTGTCATCTATGGGTAGTCCAACTATAGTTATGACTCTGCGGCAAGGTAGAGGTATGGATGCAAGGCATGGTGCAAAGACACAGGATGAGGAAGGCAGGCTGAAGTTCCACTTTCAGTCTTTTGTATCCTTCAATGTAGCAAACAGGACAATTATGGCCCTATGGAAGGCTAGATCTTTGAGTCCCGAGCAGAAGGTGCAAATAATAGAAGAAGAATCGGAAGTCAAGAGCCTCCAAACTGATGAGAGTGGGTCTTTCTTGGGCCTTGATGATGTCAGCATGTCTGAGGTTCATTCATCCTCACATGCAGTTCCTGCGAATTTCTTCGTAGAGCTATTTGGTGGAGGTGATTTGGATCGTAGAGTTATGGAGAAAGCTGGATGTCTTAACTATTCTCATACACCATGGGAATCAGAGAAGGGCGATGTGTATGTGAGGCAAATATATTACAGATATGACAAACGTGTTTCCCAATATAGAGGAGAGGTGACCAGTACTCAGCAAAAATCCTGCCTTTCTGATAAAAATGGTTGGCTTTTTCAAGAGGTCATGACACTCCATGCAATTCCACTCGGTGACTATTTCAATGTTCACATCCGATACCAAATCGAGGACACACCACCAGGGTGTCAGGTAAAAGTATCCTTTGGCATTGAGTGGCTCAAAAGCACCAAGCATCAGAAAAGAATTACAAAAAATGTTCTCAAGAATCTGCAAGATCGCCTAAAGGTCTCTTTCGCTGTAGTTGAGAAGGAGTTCACAACAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1013

Amino Acids

113.85

Weight (kDa)

6.06

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 94 1.3e-29 C2 domain
VASt PF16016 255 - 401 8.3e-32 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 536 - 637 3.2e-16 C2 domain
GRAM PF02893 689 - 796 3.2e-17 GRAM domain
VASt PF16016 861 - 992 2.1e-23 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2003
AasI GACNNNNNNGTC 3 cut(s) 233, 437, 712
AatII GACGTC 1 cut(s) 441
AbsI CCTCGAGG 1 cut(s) 457
Acc36I ACCTGC 1 cut(s) 601
AccB1I GGYRCC 1 cut(s) 303
AccB7I CCANNNNNTGG 1 cut(s) 1297
AccI GTMKAC 3 cut(s) 168, 556, 642
AciI CCGC 7 cut(s) 348, 675, 848, 1141, 1540, 2155, 2308
AclI AACGTT 1 cut(s) 1248
AclWI GGATC 9 cut(s) 42, 57, 62, 675, 777, 1272, 1285, 1664, 2631
AcsI RAATTY 9 cut(s) 248, 890, 1147, 1481, 1803, 1956, 2099, 2194, 2587
AcuI CTGAAG 1 cut(s) 2390
AcyI GRCGYC 1 cut(s) 438
AdeI CACNNNGTG 2 cut(s) 1478, 2843
AfaI GTAC 8 cut(s) 74, 218, 591, 1120, 1344, 1404, 2043, 2764
AfiI CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1297, 1913, 2150, 2233, 2434, 2878
AflIII ACRYGT 3 cut(s) 1519, 2120, 2731
AjnI CCWGG 7 cut(s) 751, 884, 1087, 1290, 1440, 1500, 2890
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AleI CACNNNNGTG 1 cut(s) 2893
Alw21I GWGCWC 2 cut(s) 189, 1163
Alw26I GTCTC 3 cut(s) 262, 1375, 3004
AlwI GGATC 9 cut(s) 42, 57, 62, 675, 777, 1272, 1285, 1664, 2631
AlwNI CAGNNNCTG 1 cut(s) 2582
Ama87I CYCGRG 4 cut(s) 30, 457, 1590, 2453
ApaI GGGCCC 1 cut(s) 31
ApeKI GCWGC 5 cut(s) 240, 724, 935, 1099, 1643
ApoI RAATTY 9 cut(s) 248, 890, 1147, 1481, 1803, 1956, 2099, 2194, 2587
ArsI GACNNNNNNTTYG 2 cut(s) 240, 272
Asp700I GAANNNNTTC 3 cut(s) 2247, 2591, 3023
AspLEI GCGC 2 cut(s) 446, 844
AsuC2I CCSGG 3 cut(s) 31, 32, 1660
AsuHPI GGTGA 8 cut(s) 94, 130, 425, 444, 1490, 2627, 2767, 2855
AsuNHI GCTAGC 2 cut(s) 844, 938
AvaI CYCGRG 4 cut(s) 30, 457, 1590, 2453
AvaII GGWCC 4 cut(s) 881, 973, 1288, 1820
BaeGI GKGCMC 1 cut(s) 31
BamHI GGATCC 1 cut(s) 1277
BanI GGYRCC 1 cut(s) 303
BanII GRGCYC 2 cut(s) 31, 189
BauI CACGAG 1 cut(s) 717
BbsI GAAGAC 3 cut(s) 131, 259, 710
Bbv12I GWGCWC 2 cut(s) 189, 1163
BbvCI CCTCAGC 2 cut(s) 176, 651
BbvI GCAGC 5 cut(s) 227, 736, 947, 1086, 1655
BccI CCATC 6 cut(s) 16, 1111, 1393, 1592, 1811, 2005
BceAI ACGGC 3 cut(s) 73, 495, 1012
BciT130I CCWGG 7 cut(s) 753, 886, 1089, 1292, 1442, 1502, 2892
BciVI GTATCC 3 cut(s) 481, 2404, 2920
BclI TGATCA 2 cut(s) 415, 1573
BcnI CCSGG 3 cut(s) 31, 32, 1660
BcoDI GTCTC 3 cut(s) 262, 1375, 3004
BfaI CTAG 8 cut(s) 615, 845, 939, 1020, 1194, 1704, 1710, 2439
BfmI CTRYAG 6 cut(s) 864, 1154, 1307, 2147, 2292, 3009
BfoI RGCGCY 2 cut(s) 447, 845
BfuAI ACCTGC 1 cut(s) 601
BfuI GTATCC 3 cut(s) 481, 2404, 2920
BglII AGATCT 1 cut(s) 2441
BisI GCNGC 8 cut(s) 241, 349, 725, 936, 1100, 1644, 2155, 2309
BlsI GCNGC 8 cut(s) 242, 350, 726, 937, 1101, 1645, 2156, 2310
BmcAI AGTACT 2 cut(s) 218, 2764
Bme18I GGWCC 4 cut(s) 881, 973, 1288, 1820
BmeT110I CYCGRG 4 cut(s) 30, 457, 1590, 2453
BmiI GGNNCC 6 cut(s) 28, 29, 305, 883, 1279, 2186
BmsI GCATC 5 cut(s) 1089, 1310, 1756, 2317, 2953
BmtI GCTAGC 2 cut(s) 848, 942
BpiI GAAGAC 3 cut(s) 131, 259, 710
BplI GAGNNNNNCTC 2 cut(s) 656, 688
BpmI CTGGAG 2 cut(s) 296, 1463
Bpu10I CCTNAGC 4 cut(s) 176, 651, 1095, 1926
BpuEI CTTGAG 2 cut(s) 1972, 2957
BpuMI CCSGG 3 cut(s) 31, 32, 1660
BsaAI YACGTR 1 cut(s) 76
BsaBI GATNNNNATC 1 cut(s) 2622
BsaHI GRCGYC 1 cut(s) 438
BsaI GGTCTC 2 cut(s) 1375, 3004
BsaJI CCNNGG 8 cut(s) 30, 663, 884, 969, 1088, 1273, 2672, 2891
BsaXI ACNNNNNCTCC 6 cut(s) 418, 448, 1506, 1536, 1971, 2001
Bsc4I CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1297, 1913, 2150, 2233, 2434, 2878
Bse1I ACTGG 1 cut(s) 2760
Bse3DI GCAATG 5 cut(s) 51, 645, 1699, 1776, 1990
Bse8I GATNNNNATC 1 cut(s) 2622
BseBI CCWGG 7 cut(s) 753, 886, 1089, 1292, 1442, 1502, 2892
BseDI CCNNGG 8 cut(s) 30, 663, 884, 969, 1088, 1273, 2672, 2891
BseGI GGATG 9 cut(s) 856, 1149, 1780, 1801, 2332, 2359, 2564, 2654, 2862
BseJI GATNNNNATC 1 cut(s) 2622
BseLI CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1297, 1913, 2150, 2233, 2434, 2878
BseMI GCAATG 5 cut(s) 51, 645, 1699, 1776, 1990
BseNI ACTGG 1 cut(s) 2760
BseRI GAGGAG 5 cut(s) 179, 771, 1608, 1992, 2763
BseSI GKGCMC 1 cut(s) 31
BseXI GCAGC 5 cut(s) 227, 736, 947, 1086, 1655
BseYI CCCAGC 3 cut(s) 88, 1424, 1525
BsgI GTGCAG 1 cut(s) 1580
BshNI GGYRCC 1 cut(s) 303
BsiHKAI GWGCWC 2 cut(s) 189, 1163
BsiHKCI CYCGRG 4 cut(s) 30, 457, 1590, 2453
BsiSI CCGG 3 cut(s) 31, 41, 1659
BsiWI CGTACG 1 cut(s) 72
BslFI GGGAC 2 cut(s) 443, 2436
BslI CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1297, 1913, 2150, 2233, 2434, 2878
BsmAI GTCTC 3 cut(s) 262, 1375, 3004
BsmBI CGTCTC 1 cut(s) 262
BsmFI GGGAC 2 cut(s) 443, 2436
BsmI GAATGC 1 cut(s) 2066
Bso31I GGTCTC 2 cut(s) 1375, 3004
BsoBI CYCGRG 4 cut(s) 30, 457, 1590, 2453
Bsp120I GGGCCC 1 cut(s) 27
Bsp1286I GDGCHC 3 cut(s) 31, 189, 1163
Bsp1407I TGTACA 1 cut(s) 1118
Bsp19I CCATGG 1 cut(s) 2672
BspACI CCGC 7 cut(s) 348, 675, 848, 1141, 1540, 2155, 2308
BspHI TCATGA 1 cut(s) 2817
BspLI GGNNCC 6 cut(s) 28, 29, 305, 883, 1279, 2186
BspMAI CTGCAG 3 cut(s) 1158, 1311, 2151
BspMI ACCTGC 1 cut(s) 601
BspOI GCTAGC 2 cut(s) 848, 942
BspPI GGATC 9 cut(s) 42, 57, 62, 675, 777, 1272, 1285, 1664, 2631
BspQI GCTCTTC 1 cut(s) 1079
BspT107I GGYRCC 1 cut(s) 303
BspTNI GGTCTC 2 cut(s) 1375, 3004
BsrDI GCAATG 5 cut(s) 51, 645, 1699, 1776, 1990
BsrGI TGTACA 1 cut(s) 1118
BsrI ACTGG 1 cut(s) 2760
BssECI CCNNGG 8 cut(s) 30, 663, 884, 969, 1088, 1273, 2672, 2891
BssNI GRCGYC 1 cut(s) 438
BssSI CACGAG 1 cut(s) 717
BssT1I CCWWGG 2 cut(s) 1273, 2672
Bst2BI CACGAG 1 cut(s) 717
Bst2UI CCWGG 7 cut(s) 753, 886, 1089, 1292, 1442, 1502, 2892
Bst4CI ACNGT 5 cut(s) 434, 1214, 1366, 1615, 2041
Bst6I CTCTTC 3 cut(s) 144, 1079, 1914
BstACI GRCGYC 1 cut(s) 438
BstAPI GCANNNNNTGC 2 cut(s) 2464, 2582
BstAUI TGTACA 1 cut(s) 1118
BstBAI YACGTR 1 cut(s) 76
BstDSI CCRYGG 1 cut(s) 2672
BstEII GGTNACC 1 cut(s) 2755
BstENI CCTNNNNNAGG 4 cut(s) 492, 1911, 2231, 2432
BstF5I GGATG 9 cut(s) 856, 1149, 1780, 1801, 2332, 2359, 2564, 2654, 2862
BstH2I RGCGCY 2 cut(s) 447, 845
BstHHI GCGC 2 cut(s) 446, 844
BstMAI GTCTC 3 cut(s) 262, 1375, 3004
BstMWI GCNNNNNNNGC 8 cut(s) 13, 94, 962, 1016, 1099, 1462, 2464, 2582
BstNI CCWGG 7 cut(s) 753, 886, 1089, 1292, 1442, 1502, 2892
BstNSI RCATGY 3 cut(s) 2124, 2551, 2576
BstPI GGTNACC 1 cut(s) 2755
BstSFI CTRYAG 6 cut(s) 864, 1154, 1307, 2147, 2292, 3009
BstSLI GKGCMC 1 cut(s) 31
BstV1I GCAGC 5 cut(s) 227, 736, 947, 1086, 1655
BstV2I GAAGAC 3 cut(s) 131, 259, 710
BstX2I RGATCY 4 cut(s) 34, 49, 1277, 2441
BstYI RGATCY 4 cut(s) 34, 49, 1277, 2441
BsuI GTATCC 3 cut(s) 481, 2404, 2920
BtgI CCRYGG 1 cut(s) 2672
BtgZI GCGATG 1 cut(s) 2706
BtsCI GGATG 9 cut(s) 856, 1149, 1780, 1801, 2332, 2359, 2564, 2654, 2862
BtsI GCAGTG 2 cut(s) 1304, 1576
BtsIMutI CAGTG 4 cut(s) 439, 1304, 1362, 1576
BveI ACCTGC 1 cut(s) 601
CaiI CAGNNNCTG 1 cut(s) 2582
CciI TCATGA 1 cut(s) 2817
CfoI GCGC 2 cut(s) 446, 844
Cfr9I CCCGGG 1 cut(s) 30
CseI GACGC 1 cut(s) 232
CsiI ACCWGGT 1 cut(s) 1290
Csp6I GTAC 8 cut(s) 73, 217, 590, 1119, 1343, 1403, 2042, 2763
CviQI GTAC 8 cut(s) 73, 217, 590, 1119, 1343, 1403, 2042, 2763
DraI TTTAAA 1 cut(s) 1206
DraIII CACNNNGTG 2 cut(s) 1478, 2843
DrdI GACNNNNNNGTC 3 cut(s) 233, 437, 712
DseDI GACNNNNNNGTC 3 cut(s) 233, 437, 712
Eam1104I CTCTTC 3 cut(s) 144, 1079, 1914
EarI CTCTTC 3 cut(s) 144, 1079, 1914
EciI GGCGGA 1 cut(s) 1156
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 2 cut(s) 1273, 2672
Eco147I AGGCCT 1 cut(s) 1440
Eco24I GRGCYC 2 cut(s) 31, 189
Eco31I GGTCTC 2 cut(s) 1375, 3004
Eco32I GATATC 1 cut(s) 600
Eco47I GGWCC 4 cut(s) 881, 973, 1288, 1820
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 2390
Eco88I CYCGRG 4 cut(s) 30, 457, 1590, 2453
Eco91I GGTNACC 1 cut(s) 2755
EcoICRI GAGCTC 1 cut(s) 187
EcoNI CCTNNNNNAGG 4 cut(s) 492, 1911, 2231, 2432
EcoO109I RGGNCCY 2 cut(s) 27, 881
EcoO65I GGTNACC 1 cut(s) 2755
EcoRI GAATTC 1 cut(s) 1481
EcoRII CCWGG 7 cut(s) 751, 884, 1087, 1290, 1440, 1500, 2890
EcoRV GATATC 1 cut(s) 600
EcoT14I CCWWGG 2 cut(s) 1273, 2672
EcoT38I GRGCYC 2 cut(s) 31, 189
ErhI CCWWGG 2 cut(s) 1273, 2672
Esp3I CGTCTC 1 cut(s) 262
FalI AAGNNNNNCTT 4 cut(s) 113, 145, 2363, 2395
FaqI GGGAC 2 cut(s) 443, 2436
FauI CCCGC 1 cut(s) 1547
FauNDI CATATG 2 cut(s) 1045, 1675
FbaI TGATCA 2 cut(s) 415, 1573
FblI GTMKAC 3 cut(s) 168, 556, 642
Fnu4HI GCNGC 8 cut(s) 241, 349, 725, 936, 1100, 1644, 2155, 2309
FokI GGATG 9 cut(s) 863, 1156, 1787, 1808, 2339, 2366, 2551, 2661, 2849
FriOI GRGCYC 2 cut(s) 31, 189
Fsp4HI GCNGC 8 cut(s) 241, 349, 725, 936, 1100, 1644, 2155, 2309
FspBI CTAG 8 cut(s) 615, 845, 939, 1020, 1194, 1704, 1710, 2439
GlaI GCGC 2 cut(s) 445, 843
GluI GCNGC 8 cut(s) 241, 349, 725, 936, 1100, 1644, 2155, 2309
GsaI CCCAGC 3 cut(s) 92, 1428, 1529
GsuI CTGGAG 2 cut(s) 296, 1463
HaeII RGCGCY 2 cut(s) 447, 845
HapII CCGG 3 cut(s) 31, 41, 1659
HgaI GACGC 1 cut(s) 232
HhaI GCGC 2 cut(s) 446, 844
Hin1I GRCGYC 1 cut(s) 438
Hin6I GCGC 2 cut(s) 444, 842
HinP1I GCGC 2 cut(s) 444, 842
HincII GTYRAC 3 cut(s) 169, 557, 643
HindII GTYRAC 3 cut(s) 169, 557, 643
HpaII CCGG 3 cut(s) 31, 41, 1659
HphI GGTGA 8 cut(s) 94, 130, 425, 444, 1490, 2627, 2767, 2855
Hpy166II GTNNAC 7 cut(s) 169, 557, 643, 1121, 1405, 2860, 3027
Hpy8I GTNNAC 7 cut(s) 169, 557, 643, 1121, 1405, 2860, 3027
Hpy99I CGWCG 6 cut(s) 173, 185, 230, 239, 443, 998
HpyCH4III ACNGT 5 cut(s) 434, 1214, 1366, 1615, 2041
HpyCH4IV ACGT 6 cut(s) 75, 438, 1248, 1519, 2044, 2731
HpyF10VI GCNNNNNNNGC 8 cut(s) 13, 94, 962, 1016, 1099, 1462, 2464, 2582
HpySE526I ACGT 6 cut(s) 75, 438, 1248, 1519, 2044, 2731
Hsp92I GRCGYC 1 cut(s) 438
HspAI GCGC 2 cut(s) 444, 842
Ksp22I TGATCA 2 cut(s) 415, 1573
LguI GCTCTTC 1 cut(s) 1079
LmnI GCTCC 2 cut(s) 192, 1190
Lsp1109I GCAGC 5 cut(s) 227, 736, 947, 1086, 1655
LweI GCATC 5 cut(s) 1089, 1310, 1756, 2317, 2953
MabI ACCWGGT 1 cut(s) 1290
MaeI CTAG 8 cut(s) 615, 845, 939, 1020, 1194, 1704, 1710, 2439
MaeII ACGT 6 cut(s) 75, 438, 1248, 1519, 2044, 2731
MaeIII GTNAC 8 cut(s) 434, 714, 781, 919, 1733, 2123, 2755, 2843
MflI RGATCY 4 cut(s) 34, 49, 1277, 2441
MhlI GDGCHC 3 cut(s) 31, 189, 1163
MlyI GAGTC 5 cut(s) 174, 538, 1590, 2296, 2458
MmeI TCCRAC 3 cut(s) 2005, 2288, 2312
MroXI GAANNNNTTC 3 cut(s) 2247, 2591, 3023
MseI TTAA 8 cut(s) 131, 363, 930, 1205, 1251, 1964, 1992, 2655
MslI CAYNNNNRTG 2 cut(s) 1166, 2893
MspA1I CMGCKG 2 cut(s) 1259, 1646
MspI CCGG 3 cut(s) 31, 41, 1659
Mva1269I GAATGC 1 cut(s) 2066
MvaI CCWGG 7 cut(s) 753, 886, 1089, 1292, 1442, 1502, 2892
MwoI GCNNNNNNNGC 8 cut(s) 13, 94, 962, 1016, 1099, 1462, 2464, 2582
NciI CCSGG 3 cut(s) 31, 32, 1660
NcoI CCATGG 1 cut(s) 2672
NdeI CATATG 2 cut(s) 1045, 1675
NheI GCTAGC 2 cut(s) 844, 938
NlaIV GGNNCC 6 cut(s) 28, 29, 305, 883, 1279, 2186
NmuCI GTSAC 6 cut(s) 434, 714, 1733, 2123, 2755, 2843
NspI RCATGY 3 cut(s) 2124, 2551, 2576
OliI CACNNNNGTG 1 cut(s) 2893
PaeR7I CTCGAG 2 cut(s) 457, 1590
PagI TCATGA 1 cut(s) 2817
PceI AGGCCT 1 cut(s) 1440
PciI ACATGT 1 cut(s) 2120
PciSI GCTCTTC 1 cut(s) 1079
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 231, 240
PctI GAATGC 1 cut(s) 2066
PdmI GAANNNNTTC 3 cut(s) 2247, 2591, 3023
Pfl23II CGTACG 1 cut(s) 72
PflFI GACNNNGTC 1 cut(s) 233
PflMI CCANNNNNTGG 1 cut(s) 1297
PkrI GCNGC 8 cut(s) 242, 350, 726, 937, 1101, 1645, 2156, 2310
PleI GAGTC 5 cut(s) 173, 537, 1589, 2296, 2457
PpsI GAGTC 5 cut(s) 173, 537, 1589, 2296, 2457
Ppu21I YACGTR 1 cut(s) 76
PpuMI RGGWCCY 1 cut(s) 881
PscI ACATGT 1 cut(s) 2120
PsiI TTATAA 1 cut(s) 2003
Psp124BI GAGCTC 1 cut(s) 189
Psp1406I AACGTT 1 cut(s) 1248
Psp5II RGGWCCY 1 cut(s) 881
Psp6I CCWGG 7 cut(s) 751, 884, 1087, 1290, 1440, 1500, 2890
PspEI GGTNACC 1 cut(s) 2755
PspFI CCCAGC 3 cut(s) 88, 1424, 1525
PspGI CCWGG 7 cut(s) 751, 884, 1087, 1290, 1440, 1500, 2890
PspLI CGTACG 1 cut(s) 72
PspN4I GGNNCC 6 cut(s) 28, 29, 305, 883, 1279, 2186
PspOMI GGGCCC 1 cut(s) 27
PspPPI RGGWCCY 1 cut(s) 881
PspXI VCTCGAGB 2 cut(s) 457, 1590
PstI CTGCAG 3 cut(s) 1158, 1311, 2151
PstNI CAGNNNCTG 1 cut(s) 2582
PsuI RGATCY 4 cut(s) 34, 49, 1277, 2441
PsyI GACNNNGTC 1 cut(s) 233
PvuII CAGCTG 2 cut(s) 1259, 1646
RsaI GTAC 8 cut(s) 74, 218, 591, 1120, 1344, 1404, 2043, 2764
RsaNI GTAC 8 cut(s) 73, 217, 590, 1119, 1343, 1403, 2042, 2763
RseI CAYNNNNRTG 2 cut(s) 1166, 2893
SacI GAGCTC 1 cut(s) 189
SalI GTCGAC 3 cut(s) 167, 555, 641
SapI GCTCTTC 1 cut(s) 1079
SaqAI TTAA 8 cut(s) 131, 363, 930, 1205, 1251, 1964, 1992, 2655
SatI GCNGC 8 cut(s) 241, 349, 725, 936, 1100, 1644, 2155, 2309
SbfI CCTGCAGG 1 cut(s) 2151
ScaI AGTACT 2 cut(s) 218, 2764
SchI GAGTC 5 cut(s) 174, 538, 1590, 2296, 2458
SdaI CCTGCAGG 1 cut(s) 2151
SduI GDGCHC 3 cut(s) 31, 189, 1163
SexAI ACCWGGT 1 cut(s) 1290
SfaNI GCATC 5 cut(s) 1089, 1310, 1756, 2317, 2953
SfcI CTRYAG 6 cut(s) 864, 1154, 1307, 2147, 2292, 3009
Sfr274I CTCGAG 2 cut(s) 457, 1590
SinI GGWCC 4 cut(s) 881, 973, 1288, 1820
SlaI CTCGAG 2 cut(s) 457, 1590
SmaI CCCGGG 1 cut(s) 32
SmiMI CAYNNNNRTG 2 cut(s) 1166, 2893
SmlI CTYRAG 4 cut(s) 457, 1590, 1951, 2972
SmoI CTYRAG 4 cut(s) 457, 1590, 1951, 2972
Sse8387I CCTGCAGG 1 cut(s) 2151
SseBI AGGCCT 1 cut(s) 1440
SsiI CCGC 7 cut(s) 348, 675, 848, 1141, 1540, 2155, 2308
SspI AATATT 2 cut(s) 1349, 1755
SspMI CTAG 8 cut(s) 615, 845, 939, 1020, 1194, 1704, 1710, 2439
SstI GAGCTC 1 cut(s) 189
StuI AGGCCT 1 cut(s) 1440
StyI CCWWGG 2 cut(s) 1273, 2672
TaaI ACNGT 5 cut(s) 434, 1214, 1366, 1615, 2041
TaiI ACGT 6 cut(s) 78, 441, 1251, 1522, 2047, 2734
TaqI TCGA 9 cut(s) 168, 270, 291, 458, 556, 642, 1591, 1827, 2877
TatI WGTACW 4 cut(s) 216, 1118, 1342, 2762
TauI GCSGC 3 cut(s) 351, 2157, 2311
Tru1I TTAA 8 cut(s) 131, 363, 930, 1205, 1251, 1964, 1992, 2655
Tru9I TTAA 8 cut(s) 131, 363, 930, 1205, 1251, 1964, 1992, 2655
TscAI CASTG 4 cut(s) 439, 1311, 1369, 1576
TseFI GTSAC 6 cut(s) 434, 714, 1733, 2123, 2755, 2843
TseI GCWGC 5 cut(s) 240, 724, 935, 1099, 1643
Tsp45I GTSAC 6 cut(s) 434, 714, 1733, 2123, 2755, 2843
TspGWI ACGGA 2 cut(s) 60, 1079
TspMI CCCGGG 1 cut(s) 30
TspRI CASTG 4 cut(s) 439, 1311, 1369, 1576
Tth111I GACNNNGTC 1 cut(s) 233
Van91I CCANNNNNTGG 1 cut(s) 1297
VpaK11BI GGWCC 4 cut(s) 881, 973, 1288, 1820
XagI CCTNNNNNAGG 4 cut(s) 492, 1911, 2231, 2432
XapI RAATTY 9 cut(s) 248, 890, 1147, 1481, 1803, 1956, 2099, 2194, 2587
XceI RCATGY 3 cut(s) 2124, 2551, 2576
XcmI CCANNNNNNNNNTGG 1 cut(s) 2078
XhoI CTCGAG 2 cut(s) 457, 1590
XmaI CCCGGG 1 cut(s) 30
XmiI GTMKAC 3 cut(s) 168, 556, 642
XmnI GAANNNNTTC 3 cut(s) 2247, 2591, 3023
XspI CTAG 8 cut(s) 615, 845, 939, 1020, 1194, 1704, 1710, 2439
ZraI GACGTC 1 cut(s) 439
ZrmI AGTACT 2 cut(s) 218, 2764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.