RchiOBHm_Chr5g0000311

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006300
Physical Location & Seq
Reverse (-)
188784 .. 194664
5881 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28188

Sequence Viewer

Length: 3066 bp
ATGAAGCTGGTGGTTCAAGTGATGGGGGCTCGAGATCTGCCGGCAATGGATCTAAACGGGCTGAGTGATCCGTACGTGAAGGTGAAGATGGGAAAGCAGAAGTTCAAGACCAAAGTGGTGAAGAAGACGTTAAACCCATATTGGGGTGAAGAGTTCACGTTTCGAGTCGACGACCTCAACGACGAGCTCCTCATCTCTTGTTTGGATGAAGATAAGTACTTCAACGACGACTTCGTGGGCTGCGTCAAGTTCCCTGTTTCCCAAGTCTTCGATTCCCCCAACAAGTGCCTCGACACTTGTTGGCACCCTCTCCAGCCCAAGAGCAAGAAGCCCAAGCACAAGGATTGCGGCGAGATTCTTCTTAATATCTCTCTCAGTACAAACAATGCATTCTCCGACTCCGCTTCCGATGGTGATCACATCAGGAGGGATTCCGACATTGGAGGTGAATCACCCTCAAGGTTATCCGAAACGGCTTCCCCTCACAGAGGCAGGCTTGATGAACTTCCATTCTCCAAGGAAGATAAAGAAAAGGAAAAGAGTACTCTTGCCCAGAAATCCCTTGCTGGCCGACTTGTTCAGATGTTCAATAAAAATCCTGACGCACCTCCAATATCTTCAAACCACTCTAGCAAAGCTGACTTGACTGAGCTTGTTGACATTTCTGAGGCCACTTATTCTGAGGATCACTCCTCTTGTGTTCCTTTTGAAGAGCTAATGAAAACAATGCAGTTACGCGATCAGGCTACTGAAACCCCCGAGAACCTACCCGGTGGAGTGCTTCTAGATCAAATGTATGTTACTGAACCCAAACACCTCAACACTTTACTCTTTTCTCCGGATTCTACTTTTCCAAAGGCGTTGGCAGATCTGCATGGAACTACAGAATTGGAACAAGGACCCTGGAAATTTGAAAACGACAGCTTAAAAAGAGTGGTTACATATGTTAAGGCAGCTAGCAAATTAATCAAGGCTTGCAAAGGAAGTGAGGACCAAACATATCTCAAAGCCGATGGGAAAGTTTTTGCAGTTTTATCAAGTGTGAGCACTCCTGATGTTCCATATGGGAAAACTTTTAGGACGGAGTTGCTCTACTGCATTACACCTGGGCCTGAGCTGCCATCGGGAGAACAATCTACACGCTTTGTAATATCCTGGCGCATGAATTTTCTGCAGAGCACCATGATGAAAGGAATGATAGAAAATGGAGCTCGGCAAGGTTTAAAGGACAGTTATGAGCAGTATGCAACTTTATTATCGCAGAACGTTAAGCCAGCTGATTCTAAAGACCTTGGATCCAATAAGGACCAGGTTTTGGCATCATTGCAGGCTGAATCCCAGTCTGATTGGAAGCTGGCAGTACAATATTTTGCTAATTTCACTGTGGTCTCCACATTTTTTATTGGATTGTATATGCTGGTACACATCTACCTGGCTACACCCAGCACTATTCAAGGGTTGGAGTTTGTTGGGCTTGACTTGCCAGATTCAATTGGTGAATTCATTGTTTGTGGTGTCCTGGTACTTCAAGGAGAACGTGTGCTGGGTTTGATCTCACGCTTTATGCAGGCCAGAGTGCAGAAAGGCAGTGACCATGGAGTCAAAGCCCGAGGAGATGGTTGGTTGCTAACTGTTGCCTTGATTGAAGGAAGTAATATAGCAGCTGTTGATTCAAGTGGGTTCTCTGATCCATACGTGGTGTTTTCTTGCAATGGAAAAGCTAGAACTAGCTCAATCAAGTTCCAGAAATGCGACCCTATGTGGAATGAAATATTTGAGTTTGATGCAATGGATGAGCCTCCTTCTGTGCTGGATGTGGAAATTTATGATTTTGATGGTCCTTTTGATGAAGCTACCTCTCTGGGCCATGCTGAAATCAATTTTGTGAAAACCAATATATCAGATTTAGCTGATATGTGGATTCCTCTTCAAGGAAAGTTAGCTCAGGCTTGTCAGTCAAAACTCCACTTGAGAATTTTCTTAAACAATACAAGAGGTGGCAATGTTGTTAATCACTTTTTAAATAAGATGGAGAAGGAGGTTGGAAAGAAGATTACTGTACGTTCTCCTCAAACAAATTCAGCATTCCAAAAACTCTTTGGGCTTCCACCAGAGGAATTTCTTATCAACGACTTTACATGTCACTTGAAACGAAAAATGCCCCTGCAGGGCCGCCTATTTCTTTCTGCGAGAATAATTGGGTTCCATGCAAATTTATTTGGTCGCAAGACAAAGTTCTTTTTCCTCTGGGAGGATATAGAAGATATTCATATTGTTCCTCCAACTCTGTCATCTATGGGTAGTCCAACTATAGTCATGACTCTGCGGCAAGGTAGAGGTATGGATGCAAGGCATGGGGCAAAGACACAGGATGAGGAAGGCAGGCTGAAGTTCCACTTCCAGTCCTTCGTATCCTTCAATGTAGCGAACAGGACAATTATGGCCCTGTGGAAGGCTAGATCTTTGAGTCCTGAGCAGAAGGTGCAAATAATAGAAGAAGAATCAGAAGTCAAGAGCCTCCAAACTGAAGAGAGTGGGTCTTTCTTGGGCCTTGATGATGTCAGCATGTCTGAGGTTTATTCATCCTTGCATTCAGTTCCTACAAATTTCTTCGCAGAGCTATTCGGTGGAGGTGAATTGGATCGTAGAGTTATGGAGAAAGCTGGTTGTCTTAACTATTCTCACACCCCATGGGAATCAGAGAAGGGTGATGTCTATGTGAGGCAAATATATTACAGATATGACAAACGTGTTTCCCAATATAGAGGAGAGGTGACTAGTACTCAGCAAAAATCCCGCCTTTCTGATAGAAATGGTTGGCTTTTTCAAGAAGTCATGACCCTCCATGCAATTCCACTTGGTGACTATTTCAATGTTCACATCCGATACCAAATGGAGGACTCGTCCTCAACACCACCAGGGTGTCTGGTAAAAGTATACTTTGGCATTGAGTGGCTCAAAAGCACTAAGCATCAGAAAAGAATTACAAAAAATGTTCTCAAGAATCTGCAAGATCGCCTAAAGGTCTCCTTTGCTGTAGTTGAGAAGGAGTTCACATCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1021

Amino Acids

115.02

Weight (kDa)

6.09

Isoelectric Point (pI)

47.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 93 2e-29 C2 domain
VASt PF16016 261 - 407 3.3e-32 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 542 - 643 4e-16 C2 domain
GRAM PF02893 695 - 802 3.2e-17 GRAM domain
VASt PF16016 867 - 1001 1.6e-22 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1598
AccB1I GGYRCC 1 cut(s) 303
AccB7I CCANNNNNTGG 1 cut(s) 1315
AccI GTMKAC 2 cut(s) 168, 2937
AccII CGCG 1 cut(s) 738
AccIII TCCGGA 1 cut(s) 838
AciI CCGC 5 cut(s) 348, 402, 2173, 2326, 2797
AclI AACGTT 1 cut(s) 1266
AclWI GGATC 7 cut(s) 57, 62, 693, 1290, 1303, 1682, 2649
AcoI YGGCCR 1 cut(s) 568
AcsI RAATTY 9 cut(s) 908, 1165, 1499, 1821, 1974, 2077, 2117, 2212, 2605
AcuI CTGAAG 2 cut(s) 2408, 2547
AdeI CACNNNGTG 1 cut(s) 2861
AfaI GTAC 9 cut(s) 74, 218, 379, 544, 1362, 1422, 1524, 2061, 2782
AfiI CCNNNNNNNGG 9 cut(s) 142, 143, 488, 1315, 1931, 2168, 2251, 2452, 2896
AflIII ACRYGT 3 cut(s) 1537, 2138, 2749
AhlI ACTAGT 1 cut(s) 2777
AjnI CCWGG 7 cut(s) 902, 1105, 1154, 1308, 1431, 1518, 2917
AleI CACNNNNGTG 1 cut(s) 2920
Alw21I GWGCWC 4 cut(s) 189, 1049, 1181, 1213
Alw26I GTCTC 2 cut(s) 1393, 3031
AlwI GGATC 7 cut(s) 57, 62, 693, 1290, 1303, 1682, 2649
Ama87I CYCGRG 3 cut(s) 30, 758, 1608
Aor13HI TCCGGA 1 cut(s) 838
AoxI GGCC 8 cut(s) 568, 669, 1109, 1569, 1864, 2170, 2442, 2548
ApeKI GCWGC 4 cut(s) 240, 953, 1117, 1661
ApoI RAATTY 9 cut(s) 908, 1165, 1499, 1821, 1974, 2077, 2117, 2212, 2605
AseI ATTAAT 1 cut(s) 965
Asp700I GAANNNNTTC 2 cut(s) 2265, 3050
AspLEI GCGC 1 cut(s) 1161
AspS9I GGNCC 9 cut(s) 899, 991, 1109, 1306, 1838, 1864, 2170, 2443, 2548
AsuC2I CCSGG 1 cut(s) 771
AsuNHI GCTAGC 1 cut(s) 956
AvaI CYCGRG 3 cut(s) 30, 758, 1608
AvaII GGWCC 4 cut(s) 899, 991, 1306, 1838
BamHI GGATCC 1 cut(s) 1295
BanI GGYRCC 1 cut(s) 303
BanII GRGCYC 3 cut(s) 31, 189, 1213
BbsI GAAGAC 2 cut(s) 131, 259
Bbv12I GWGCWC 4 cut(s) 189, 1049, 1181, 1213
BbvI GCAGC 4 cut(s) 227, 965, 1104, 1673
BccI CCATC 8 cut(s) 16, 82, 404, 1007, 1129, 1610, 1829, 2023
BceAI ACGGC 1 cut(s) 489
BciT130I CCWGG 7 cut(s) 904, 1107, 1156, 1310, 1433, 1520, 2919
BciVI GTATCC 1 cut(s) 2422
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 1 cut(s) 771
BcoDI GTCTC 2 cut(s) 1393, 3031
BcuI ACTAGT 1 cut(s) 2777
BfaI CTAG 7 cut(s) 630, 785, 957, 1722, 1728, 2457, 2778
BfmI CTRYAG 5 cut(s) 882, 1172, 2165, 2310, 3036
BfuI GTATCC 1 cut(s) 2422
BglII AGATCT 3 cut(s) 34, 868, 2459
BisI GCNGC 7 cut(s) 241, 349, 954, 1118, 1662, 2173, 2327
BlsI GCNGC 7 cut(s) 242, 350, 955, 1119, 1663, 2174, 2328
BmcAI AGTACT 3 cut(s) 218, 544, 2782
Bme1390I CCNGG 8 cut(s) 771, 904, 1107, 1156, 1310, 1433, 1520, 2919
Bme18I GGWCC 4 cut(s) 899, 991, 1306, 1838
BmeT110I CYCGRG 3 cut(s) 30, 758, 1608
BmgT120I GGNCC 9 cut(s) 899, 991, 1109, 1306, 1838, 1864, 2170, 2443, 2548
BmiI GGNNCC 4 cut(s) 305, 901, 1297, 2204
BmrFI CCNGG 8 cut(s) 771, 904, 1107, 1156, 1310, 1433, 1520, 2919
BmrI ACTGGG 1 cut(s) 1333
BmsI GCATC 4 cut(s) 1328, 1774, 2335, 2980
BmtI GCTAGC 1 cut(s) 960
BmuI ACTGGG 1 cut(s) 1333
BpiI GAAGAC 2 cut(s) 131, 259
BplI GAGNNNNNCTC 2 cut(s) 674, 706
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 3 cut(s) 1113, 1944, 2472
BpuEI CTTGAG 3 cut(s) 442, 1990, 2984
BpuMI CCSGG 1 cut(s) 771
BsaAI YACGTR 2 cut(s) 76, 1696
BsaBI GATNNNNATC 1 cut(s) 414
BsaI GGTCTC 2 cut(s) 1393, 3031
BsaJI CCNNGG 8 cut(s) 516, 902, 1106, 1291, 1594, 1609, 2690, 2918
BsaWI WCCGGW 1 cut(s) 838
BsaXI ACNNNNNCTCC 4 cut(s) 1524, 1554, 2888, 2918
Bsc4I CCNNNNNNNGG 9 cut(s) 142, 143, 488, 1315, 1931, 2168, 2251, 2452, 2896
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 1339, 2401
Bse3DI GCAATG 5 cut(s) 51, 1322, 1717, 1794, 2008
Bse8I GATNNNNATC 1 cut(s) 414
BseAI TCCGGA 1 cut(s) 838
BseBI CCWGG 7 cut(s) 904, 1107, 1156, 1310, 1433, 1520, 2919
BseDI CCNNGG 8 cut(s) 516, 902, 1106, 1291, 1594, 1609, 2690, 2918
BseGI GGATG 7 cut(s) 211, 1798, 1819, 2350, 2377, 2582, 2880
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 9 cut(s) 142, 143, 488, 1315, 1931, 2168, 2251, 2452, 2896
BseMI GCAATG 5 cut(s) 51, 1322, 1717, 1794, 2008
BseNI ACTGG 2 cut(s) 1339, 2401
BseRI GAGGAG 5 cut(s) 179, 682, 1626, 2058, 2781
BseXI GCAGC 4 cut(s) 227, 965, 1104, 1673
BseYI CCCAGC 2 cut(s) 1442, 1543
BsgI GTGCAG 1 cut(s) 1598
Bsh1236I CGCG 1 cut(s) 738
BshFI GGCC 8 cut(s) 570, 671, 1111, 1571, 1866, 2172, 2444, 2550
BshNI GGYRCC 1 cut(s) 303
BsiHKAI GWGCWC 4 cut(s) 189, 1049, 1181, 1213
BsiHKCI CYCGRG 3 cut(s) 30, 758, 1608
BsiSI CCGG 3 cut(s) 41, 771, 839
BsiWI CGTACG 1 cut(s) 72
BslI CCNNNNNNNGG 9 cut(s) 142, 143, 488, 1315, 1931, 2168, 2251, 2452, 2896
BsmAI GTCTC 2 cut(s) 1393, 3031
BsmI GAATGC 3 cut(s) 389, 2084, 2590
BsnI GGCC 8 cut(s) 570, 671, 1111, 1571, 1866, 2172, 2444, 2550
Bso31I GGTCTC 2 cut(s) 1393, 3031
BsoBI CYCGRG 3 cut(s) 30, 758, 1608
Bsp1286I GDGCHC 5 cut(s) 31, 189, 1049, 1181, 1213
Bsp13I TCCGGA 1 cut(s) 838
Bsp19I CCATGG 2 cut(s) 1594, 2690
BspACI CCGC 5 cut(s) 348, 402, 2173, 2326, 2797
BspANI GGCC 8 cut(s) 570, 671, 1111, 1571, 1866, 2172, 2444, 2550
BspEI TCCGGA 1 cut(s) 838
BspFNI CGCG 1 cut(s) 738
BspHI TCATGA 2 cut(s) 2316, 2835
BspLI GGNNCC 4 cut(s) 305, 901, 1297, 2204
BspMAI CTGCAG 2 cut(s) 1176, 2169
BspOI GCTAGC 1 cut(s) 960
BspPI GGATC 7 cut(s) 57, 62, 693, 1290, 1303, 1682, 2649
BspQI GCTCTTC 1 cut(s) 705
BspT107I GGYRCC 1 cut(s) 303
BspTNI GGTCTC 2 cut(s) 1393, 3031
BsrDI GCAATG 5 cut(s) 51, 1322, 1717, 1794, 2008
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 2 cut(s) 1339, 2401
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 8 cut(s) 516, 902, 1106, 1291, 1594, 1609, 2690, 2918
BssNAI GTATAC 1 cut(s) 2938
BssT1I CCWWGG 4 cut(s) 516, 1291, 1594, 2690
Bst1107I GTATAC 1 cut(s) 2938
Bst2UI CCWGG 7 cut(s) 904, 1107, 1156, 1310, 1433, 1520, 2919
Bst4CI ACNGT 4 cut(s) 1232, 1384, 1633, 2059
Bst6I CTCTTC 4 cut(s) 144, 705, 1932, 2523
BstAPI GCANNNNNTGC 1 cut(s) 2482
BstBAI YACGTR 2 cut(s) 76, 1696
BstDSI CCRYGG 2 cut(s) 1594, 2690
BstENI CCTNNNNNAGG 4 cut(s) 486, 1929, 2249, 2450
BstF5I GGATG 7 cut(s) 211, 1798, 1819, 2350, 2377, 2582, 2880
BstFNI CGCG 1 cut(s) 738
BstHHI GCGC 1 cut(s) 1161
BstMAI GTCTC 2 cut(s) 1393, 3031
BstMWI GCNNNNNNNGC 3 cut(s) 1117, 1480, 2482
BstNI CCWGG 7 cut(s) 904, 1107, 1156, 1310, 1433, 1520, 2919
BstNSI RCATGY 2 cut(s) 2142, 2569
BstSCI CCNGG 8 cut(s) 769, 902, 1105, 1154, 1308, 1431, 1518, 2917
BstSFI CTRYAG 5 cut(s) 882, 1172, 2165, 2310, 3036
BstUI CGCG 1 cut(s) 738
BstV1I GCAGC 4 cut(s) 227, 965, 1104, 1673
BstV2I GAAGAC 2 cut(s) 131, 259
BstX2I RGATCY 5 cut(s) 34, 49, 868, 1295, 2459
BstYI RGATCY 5 cut(s) 34, 49, 868, 1295, 2459
BstZ17I GTATAC 1 cut(s) 2938
BsuI GTATCC 1 cut(s) 2422
BsuRI GGCC 8 cut(s) 570, 671, 1111, 1571, 1866, 2172, 2444, 2550
BtgI CCRYGG 2 cut(s) 1594, 2690
BtsCI GGATG 7 cut(s) 211, 1798, 1819, 2350, 2377, 2582, 2880
BtsI GCAGTG 1 cut(s) 1594
BtsIMutI CAGTG 2 cut(s) 1380, 1594
CciI TCATGA 2 cut(s) 2316, 2835
CfoI GCGC 1 cut(s) 1161
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 9 cut(s) 899, 991, 1109, 1306, 1838, 1864, 2170, 2443, 2548
CseI GACGC 2 cut(s) 232, 611
CsiI ACCWGGT 1 cut(s) 1308
Csp6I GTAC 9 cut(s) 73, 217, 378, 543, 1361, 1421, 1523, 2060, 2781
CviQI GTAC 9 cut(s) 73, 217, 378, 543, 1361, 1421, 1523, 2060, 2781
DraI TTTAAA 2 cut(s) 1224, 2022
DraIII CACNNNGTG 1 cut(s) 2861
DrdI GACNNNNNNGTC 1 cut(s) 1598
DseDI GACNNNNNNGTC 1 cut(s) 1598
EaeI YGGCCR 1 cut(s) 568
Eam1104I CTCTTC 4 cut(s) 144, 705, 1932, 2523
EarI CTCTTC 4 cut(s) 144, 705, 1932, 2523
Ecl136II GAGCTC 2 cut(s) 187, 1211
Eco130I CCWWGG 4 cut(s) 516, 1291, 1594, 2690
Eco24I GRGCYC 3 cut(s) 31, 189, 1213
Eco31I GGTCTC 2 cut(s) 1393, 3031
Eco47I GGWCC 4 cut(s) 899, 991, 1306, 1838
Eco53kI GAGCTC 2 cut(s) 187, 1211
Eco57I CTGAAG 2 cut(s) 2408, 2547
Eco88I CYCGRG 3 cut(s) 30, 758, 1608
EcoICRI GAGCTC 2 cut(s) 187, 1211
EcoNI CCTNNNNNAGG 4 cut(s) 486, 1929, 2249, 2450
EcoO109I RGGNCCY 1 cut(s) 899
EcoRI GAATTC 1 cut(s) 1499
EcoRII CCWGG 7 cut(s) 902, 1105, 1154, 1308, 1431, 1518, 2917
EcoT14I CCWWGG 4 cut(s) 516, 1291, 1594, 2690
EcoT22I ATGCAT 1 cut(s) 391
EcoT38I GRGCYC 3 cut(s) 31, 189, 1213
ErhI CCWWGG 4 cut(s) 516, 1291, 1594, 2690
FalI AAGNNNNNCTT 4 cut(s) 2381, 2413, 3014, 3046
FauI CCCGC 1 cut(s) 2804
FauNDI CATATG 2 cut(s) 943, 1063
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 2 cut(s) 168, 2937
Fnu4HI GCNGC 7 cut(s) 241, 349, 954, 1118, 1662, 2173, 2327
FokI GGATG 7 cut(s) 218, 1805, 1826, 2357, 2384, 2569, 2867
FriOI GRGCYC 3 cut(s) 31, 189, 1213
Fsp4HI GCNGC 7 cut(s) 241, 349, 954, 1118, 1662, 2173, 2327
FspBI CTAG 7 cut(s) 630, 785, 957, 1722, 1728, 2457, 2778
GlaI GCGC 1 cut(s) 1160
GluI GCNGC 7 cut(s) 241, 349, 954, 1118, 1662, 2173, 2327
GsaI CCCAGC 2 cut(s) 1446, 1547
GsuI CTGGAG 1 cut(s) 296
HaeIII GGCC 8 cut(s) 570, 671, 1111, 1571, 1866, 2172, 2444, 2550
HapII CCGG 3 cut(s) 41, 771, 839
HgaI GACGC 2 cut(s) 232, 611
HhaI GCGC 1 cut(s) 1161
Hin6I GCGC 1 cut(s) 1159
HinP1I GCGC 1 cut(s) 1159
HincII GTYRAC 2 cut(s) 169, 658
HindII GTYRAC 2 cut(s) 169, 658
HpaII CCGG 3 cut(s) 41, 771, 839
Hpy166II GTNNAC 7 cut(s) 156, 169, 658, 1423, 2878, 2938, 3054
Hpy8I GTNNAC 7 cut(s) 156, 169, 658, 1423, 2878, 2938, 3054
Hpy99I CGWCG 3 cut(s) 173, 185, 230
HpyCH4III ACNGT 4 cut(s) 1232, 1384, 1633, 2059
HpyCH4IV ACGT 8 cut(s) 75, 128, 158, 1266, 1537, 1695, 2062, 2749
HpyF10VI GCNNNNNNNGC 3 cut(s) 1117, 1480, 2482
HpySE526I ACGT 8 cut(s) 75, 128, 158, 1266, 1537, 1695, 2062, 2749
HspAI GCGC 1 cut(s) 1159
Kpn2I TCCGGA 1 cut(s) 838
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Ksp22I TGATCA 1 cut(s) 415
LguI GCTCTTC 1 cut(s) 705
LmnI GCTCC 2 cut(s) 192, 1208
Lsp1109I GCAGC 4 cut(s) 227, 965, 1104, 1673
LweI GCATC 4 cut(s) 1328, 1774, 2335, 2980
MabI ACCWGGT 1 cut(s) 1308
MaeI CTAG 7 cut(s) 630, 785, 957, 1722, 1728, 2457, 2778
MaeII ACGT 8 cut(s) 75, 128, 158, 1266, 1537, 1695, 2062, 2749
MaeIII GTNAC 7 cut(s) 732, 799, 937, 1589, 2141, 2773, 2861
MfeI CAATTG 1 cut(s) 1491
MflI RGATCY 5 cut(s) 34, 49, 868, 1295, 2459
MhlI GDGCHC 5 cut(s) 31, 189, 1049, 1181, 1213
MlyI GAGTC 6 cut(s) 174, 392, 1608, 2314, 2476, 2894
MmeI TCCRAC 6 cut(s) 420, 459, 1440, 2023, 2306, 2330
Mph1103I ATGCAT 1 cut(s) 391
MroI TCCGGA 1 cut(s) 838
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 2 cut(s) 2265, 3050
MslI CAYNNNNRTG 2 cut(s) 1184, 2920
MspA1I CMGCKG 2 cut(s) 1277, 1664
MspI CCGG 3 cut(s) 41, 771, 839
MspR9I CCNGG 8 cut(s) 771, 904, 1107, 1156, 1310, 1433, 1520, 2919
MunI CAATTG 1 cut(s) 1491
Mva1269I GAATGC 3 cut(s) 389, 2084, 2590
MvaI CCWGG 7 cut(s) 904, 1107, 1156, 1310, 1433, 1520, 2919
MvnI CGCG 1 cut(s) 738
MwoI GCNNNNNNNGC 3 cut(s) 1117, 1480, 2482
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 771
NcoI CCATGG 2 cut(s) 1594, 2690
NdeI CATATG 2 cut(s) 943, 1063
NgoMIV GCCGGC 1 cut(s) 40
NheI GCTAGC 1 cut(s) 956
NlaIV GGNNCC 4 cut(s) 305, 901, 1297, 2204
NmeAIII GCCGAG 1 cut(s) 1192
NmuCI GTSAC 4 cut(s) 1589, 2141, 2773, 2861
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 2 cut(s) 2142, 2569
OliI CACNNNNGTG 1 cut(s) 2920
PaeR7I CTCGAG 1 cut(s) 30
PagI TCATGA 2 cut(s) 2316, 2835
PciI ACATGT 1 cut(s) 2138
PciSI GCTCTTC 1 cut(s) 705
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 231, 240
PctI GAATGC 3 cut(s) 389, 2084, 2590
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 2 cut(s) 2265, 3050
Pfl23II CGTACG 1 cut(s) 72
PflMI CCANNNNNTGG 1 cut(s) 1315
PkrI GCNGC 7 cut(s) 242, 350, 955, 1119, 1663, 2174, 2328
PleI GAGTC 6 cut(s) 173, 392, 1607, 2314, 2475, 2894
PpsI GAGTC 6 cut(s) 173, 392, 1607, 2314, 2475, 2894
Ppu21I YACGTR 2 cut(s) 76, 1696
PpuMI RGGWCCY 1 cut(s) 899
PscI ACATGT 1 cut(s) 2138
PshBI ATTAAT 1 cut(s) 965
Psp124BI GAGCTC 2 cut(s) 189, 1213
Psp1406I AACGTT 1 cut(s) 1266
Psp5II RGGWCCY 1 cut(s) 899
Psp6I CCWGG 7 cut(s) 902, 1105, 1154, 1308, 1431, 1518, 2917
PspFI CCCAGC 2 cut(s) 1442, 1543
PspGI CCWGG 7 cut(s) 902, 1105, 1154, 1308, 1431, 1518, 2917
PspLI CGTACG 1 cut(s) 72
PspN4I GGNNCC 4 cut(s) 305, 901, 1297, 2204
PspPI GGNCC 9 cut(s) 899, 991, 1109, 1306, 1838, 1864, 2170, 2443, 2548
PspPPI RGGWCCY 1 cut(s) 899
PstI CTGCAG 2 cut(s) 1176, 2169
PsuI RGATCY 5 cut(s) 34, 49, 868, 1295, 2459
PvuII CAGCTG 2 cut(s) 1277, 1664
RsaI GTAC 9 cut(s) 74, 218, 379, 544, 1362, 1422, 1524, 2061, 2782
RsaNI GTAC 9 cut(s) 73, 217, 378, 543, 1361, 1421, 1523, 2060, 2781
RseI CAYNNNNRTG 2 cut(s) 1184, 2920
SacI GAGCTC 2 cut(s) 189, 1213
SalI GTCGAC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 705
SatI GCNGC 7 cut(s) 241, 349, 954, 1118, 1662, 2173, 2327
Sau96I GGNCC 9 cut(s) 899, 991, 1109, 1306, 1838, 1864, 2170, 2443, 2548
SbfI CCTGCAGG 1 cut(s) 2169
ScaI AGTACT 3 cut(s) 218, 544, 2782
SchI GAGTC 6 cut(s) 174, 392, 1608, 2314, 2476, 2894
ScrFI CCNGG 8 cut(s) 771, 904, 1107, 1156, 1310, 1433, 1520, 2919
SdaI CCTGCAGG 1 cut(s) 2169
SduI GDGCHC 5 cut(s) 31, 189, 1049, 1181, 1213
SexAI ACCWGGT 1 cut(s) 1308
SfaNI GCATC 4 cut(s) 1328, 1774, 2335, 2980
SfcI CTRYAG 5 cut(s) 882, 1172, 2165, 2310, 3036
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 4 cut(s) 899, 991, 1306, 1838
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 2 cut(s) 1184, 2920
SmlI CTYRAG 4 cut(s) 30, 457, 1969, 2999
SmoI CTYRAG 4 cut(s) 30, 457, 1969, 2999
SpeI ACTAGT 1 cut(s) 2777
Sse8387I CCTGCAGG 1 cut(s) 2169
SsiI CCGC 5 cut(s) 348, 402, 2173, 2326, 2797
SspI AATATT 2 cut(s) 1367, 1773
SspMI CTAG 7 cut(s) 630, 785, 957, 1722, 1728, 2457, 2778
SstI GAGCTC 2 cut(s) 189, 1213
StyD4I CCNGG 8 cut(s) 769, 902, 1105, 1154, 1308, 1431, 1518, 2917
StyI CCWWGG 4 cut(s) 516, 1291, 1594, 2690
TaaI ACNGT 4 cut(s) 1232, 1384, 1633, 2059
TaiI ACGT 8 cut(s) 78, 131, 161, 1269, 1540, 1698, 2065, 2752
TaqI TCGA 5 cut(s) 31, 163, 168, 270, 291
TatI WGTACW 5 cut(s) 216, 377, 542, 1360, 2780
TauI GCSGC 3 cut(s) 351, 2175, 2329
TscAI CASTG 2 cut(s) 1387, 1594
TseFI GTSAC 4 cut(s) 1589, 2141, 2773, 2861
TseI GCWGC 4 cut(s) 240, 953, 1117, 1661
Tsp45I GTSAC 4 cut(s) 1589, 2141, 2773, 2861
TspGWI ACGGA 2 cut(s) 60, 1097
TspRI CASTG 2 cut(s) 1387, 1594
Van91I CCANNNNNTGG 1 cut(s) 1315
VpaK11BI GGWCC 4 cut(s) 899, 991, 1306, 1838
VspI ATTAAT 1 cut(s) 965
XagI CCTNNNNNAGG 4 cut(s) 486, 1929, 2249, 2450
XapI RAATTY 9 cut(s) 908, 1165, 1499, 1821, 1974, 2077, 2117, 2212, 2605
XbaI TCTAGA 1 cut(s) 784
XceI RCATGY 2 cut(s) 2142, 2569
XcmI CCANNNNNNNNNTGG 1 cut(s) 2096
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 2 cut(s) 168, 2937
XmnI GAANNNNTTC 2 cut(s) 2265, 3050
XspI CTAG 7 cut(s) 630, 785, 957, 1722, 1728, 2457, 2778
ZrmI AGTACT 3 cut(s) 218, 544, 2782
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.