MD10G1340200.v1.1

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006300
Physical Location & Seq
Forward (+)
41565065 .. 41571216
6152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1340200.v1.1.491

Sequence Viewer

Length: 3057 bp
ATGAAGCTGGTGGTTCAAGTATTGGGGGCTCGGGATCTACCAGCGATGGATCTCAACGGCTTCAGCGATCCTTACGTGAAGGTGCAGCTGGGGAAGCACAAGCTCAGGACCAAAGTGGTCAAGAAGACCTTAAACCCCTATTGGGGGGAAGAGTTCACCTTCAGGGTCGACGACCTCAACGACGAGCTCGTCATCTCTGTCCTCGACGAGGACAAGTACTTCAACGACGACTTCGTTGGCTCCGTCCGCATCCCTGTTTCCCAAGTCTTCGATGCCCGCAACAAGTCCCTTGAAACTGCTTGGCACCCTCTCCACCCCAAGAGTAAAAAATCCAAGAACAAGGATTGCGGCGAGATTCTTCTTGCAATACATTTTTCTTCAAACAATTCATTCGTGGACTCGGCTTCTGAGGGTGGTGACATAGGAAGTGAATCACCTTCAAGGTCTTTCAGTGGTGTTTCAGAATCAGCTTCTCCTGTGAGAGTGAGACCAGAAGAAACAGCATCTTTTAAGGACTTTAAGGAAGAAAAGATTTGTTCTCAGAAGACCTTTGCTGGTCGCATAGCTCAGATATTCAATAAAAATCCAGATTTGCTTTCAGCCTCCTCTAGCAGAGTGGACCTGTCTGAGCTGTCTGAAGCTGCTAAACCCGAAGTTTGTGAGAGCAGCCCCGAGGATCAGTCCTCTTCCGCTACATTTGAAGAATTGATGAAAACAATACAGTCACGAGATCAAGAGAGTGAAACCCCAACCAACTTACCAGGAGGAGTGCTTGTGGATCAACTGTACGTCACTCCACCCCAAGACCTAAACACATTACTATTTTCAACAGATTCAGGTTTTCAAAAAGAGGTGGCAGAAGCGCAGGGAACTACAGAATTGGATCCGGGGCAATGGAAATTAGATACCAGCACCGAGAGCGTCAAAAGAGTGGTGACATACGTTAAGGCTGCAACTAAGTTGATAAAGGCTGTCAAAGGAACTGAGGACCAAACGTATCTCAAAGCCGACGGGAAGGTTTTTGCAGTTTTAGCAAGCGTAAGCACTCCTGATGTTCCCTATGGGCGGACTTTTAAGACGGAGTTGCTCTACTGCATAACACCTGGCCCCGAGCTGCCATCTGGAGAGCAATCTTCACGTCTGGTAATATCCTGGCGGATGAATTTTCTGCAGAGCACCATGATGAAAGGAATGATAGAAAATGGAGCTCGACAAGGTTTAAAGGAAAGTTTTGACCACTATGCTACTATATTGTCTCAGAATGTTAAGCCAGTCGATTCAAAGGATCTTGGATCCAATAAGGATCAGGTTTTGGCATCACTGCAAGCTGAACCCCAGTCTGATTGGAAGCTGGCAGTACAGTATTTTGCCAATTTCCCTGTGATCTCCACATTATTTATTGGATTGTATATGTTTGTACACATCTGGCTTGCCCAACCCAGCACTATTCAAGGGTTGGAGTTTGTTGGGCTTGACTTGCCAGATTCAATTGGTGAATTCATCGTCTGTGGTGTCCTGGTTCTTCAAGGAGAAAGGGTGCTGGGGTTGATCTCACGCTTCATGCAGGCCAGAGCACAAAACGGCAGTGATCATGGAGTCAAAGCCCAAGGAGATGGTTGGTTGCTAACTGTTGCATTGATTGAAGGAAGTAACATAGCAGCTGTTGATTCGAGTGGGTTTTCTGATCCATATGTGGTGTTCACTTGCAATGGGAAAACTAGAACCAGCTCAATCAAGTTCCAGAAATGCGATCCTACATGGAATGAAATATTTGAGTTTGATGCAATGGATGAACCTCCTTCTGTGTTGGATGTGGAAGTTTATGATTTTGATGGACCTTTTGATGAAGCTATCTCTCTGGGCCATGCTGAAATCAATTTTGTAAAAACTAATATATCAGATTTAGCTGACTTGTGGGTTCCTCTTCAAGGAAAGTTAGCTCAGGCATGCCAGTCAAAACTCCACCTGAGAATTTTCTTAAACAATACAAGAGGTGGCAATGTCGTAAATCAATTTTTGACTAAGATGGAGAAGGAGGTGGGAAAGAAGATAACTGTGCGGTCTCCTCAAACAAATTCAGCATTCCAAAAACTGTTTGGGCTTCCACCGGAGGAATTTCTCATCAACGACTTTACCTGTCACTTGAAACGAAAAATGCCCCTGCAGGGCCGCCTATTTCTTTCTGCAAGAATAATTGGTTTCCATGCAAATTTATTTGGTCACAAGACAAAGTTCTTTTTCCTGTGGGAGGATATAGAGGATATTCAAGTTGTACCTCCAACTCTGTCATCTATGGGCAGTCCAATTATAGTCATGACTCTGCGGCAGGGTAGAGGTATGGATGCAAGGCATGGTGCAAAGACACAAGATGAGGAAGGCAGGCTCAAGTTCCATTTCCAGTCCTTTGTATCTTTCAATGTAGCTAACAGGACAATCATGGCTCTTTGGAAAGCTAGGTCTTTGAGTCCTGAGCAGAAGGTGAAAATAGTTGAAGAAGAATCCGATGTCAAGATCCAAGCTGAAGAGAGTGGGTCTTTCTTGGGCCTTGATGATGTCAGCATGTCTGAGGTTTATTCTGCAGCTCATTCCGTTCCTACAAATTTCTTCATAGAGCTATTTGGCGGAGGTGAATTGGACCGTAGAGTTATGGAGAAAGCTGGTTGTCTTAACTACTCTTACACCCCATGGGAATCAGAGAAGGGCGATGTCTGTGTGAGGCAAATATATTACAGATTTGATAAACGTGTGTCCCAATATAGAGGAGAGGTGACTAGTACTCAGCAAAAATCTCGCCTCCCTGATAGAAATGGTTGGCTTGTACAAGAGGTCTCGAGCCTCCATGCAGTACCACTTGGCGACTATTTCAATCTTCACGTTCGATACCAAATCGAGGATTTGCCCTCAACACCAAAGGGATGCCAGGTAAGAGTATACTTTGGGGTTGAGTGGCTCAAAAGCACTCGGCATCAGAAAAGAATTACAAAAAACGTGCTTAAGAATCTGCAAGATCGCCTGAAGGACACCTTCAGTGTAGTTGAGAAGGAATTCACGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1019

Amino Acids

114.0

Weight (kDa)

5.69

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 103 6.7e-31 C2 domain
VASt PF16016 258 - 406 6.1e-34 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 541 - 642 1.8e-16 C2 domain
GRAM PF02893 694 - 802 1.8e-19 GRAM domain
VASt PF16016 864 - 999 1.7e-22 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
AccB1I GGYRCC 1 cut(s) 303
AccI GTMKAC 2 cut(s) 168, 2931
AcsI RAATTY 8 cut(s) 1162, 1496, 1971, 2074, 2114, 2209, 2599, 3044
AcuI CTGAAG 6 cut(s) 46, 145, 657, 2541, 3010, 3035
AfaI GTAC 8 cut(s) 218, 788, 1359, 1419, 2274, 2776, 2820, 2847
AfiI CCNNNNNNNGG 9 cut(s) 142, 143, 144, 208, 1065, 1928, 2165, 2248, 2890
AflII CTTAAG 1 cut(s) 2993
AflIII ACRYGT 1 cut(s) 2743
AhlI ACTAGT 1 cut(s) 2771
AjiI CACGTC 1 cut(s) 1139
AjnI CCWGG 5 cut(s) 760, 1102, 1151, 1515, 2919
Alw21I GWGCWC 4 cut(s) 189, 1178, 1210, 1576
Alw26I GTCTC 4 cut(s) 481, 1260, 2067, 2833
Ama87I CYCGRG 4 cut(s) 30, 671, 1109, 2830
AoxI GGCC 5 cut(s) 1105, 1566, 1861, 2167, 2542
ApeKI GCWGC 7 cut(s) 85, 641, 666, 950, 1114, 1658, 2579
ApoI RAATTY 8 cut(s) 1162, 1496, 1971, 2074, 2114, 2209, 2599, 3044
Asp700I GAANNNNTTC 1 cut(s) 3044
AspLEI GCGC 1 cut(s) 865
AspS9I GGNCC 9 cut(s) 108, 619, 988, 1106, 1835, 1861, 2167, 2542, 2635
AsuC2I CCSGG 1 cut(s) 888
AsuHPI GGTGA 8 cut(s) 148, 426, 428, 946, 1505, 2491, 2639, 2779
AvaI CYCGRG 4 cut(s) 30, 671, 1109, 2830
AvaII GGWCC 5 cut(s) 108, 619, 988, 1835, 2635
BamHI GGATCC 2 cut(s) 883, 1292
BanI GGYRCC 1 cut(s) 303
BanII GRGCYC 3 cut(s) 31, 189, 1210
BauI CACGAG 1 cut(s) 726
BbsI GAAGAC 3 cut(s) 131, 259, 551
Bbv12I GWGCWC 4 cut(s) 189, 1178, 1210, 1576
BbvI GCAGC 7 cut(s) 97, 628, 678, 937, 1101, 1670, 2591
BccI CCATC 5 cut(s) 40, 1126, 1607, 1826, 2020
BceAI ACGGC 2 cut(s) 73, 1597
BcgI CGANNNNNNTGC 2 cut(s) 2771, 2805
BciT130I CCWGG 5 cut(s) 762, 1104, 1153, 1517, 2921
BclI TGATCA 1 cut(s) 1588
BcnI CCSGG 1 cut(s) 888
BcoDI GTCTC 4 cut(s) 481, 1260, 2067, 2833
BcuI ACTAGT 1 cut(s) 2771
BfaI CTAG 4 cut(s) 609, 1719, 2454, 2772
BfmI CTRYAG 4 cut(s) 873, 1169, 2162, 2577
BfrI CTTAAG 1 cut(s) 2993
BmcAI AGTACT 2 cut(s) 218, 2776
Bme1390I CCNGG 6 cut(s) 762, 888, 1104, 1153, 1517, 2921
Bme18I GGWCC 5 cut(s) 108, 619, 988, 1835, 2635
BmeT110I CYCGRG 4 cut(s) 30, 671, 1109, 2830
BmgBI CACGTC 1 cut(s) 1139
BmgT120I GGNCC 9 cut(s) 108, 619, 988, 1106, 1835, 1861, 2167, 2542, 2635
BmiI GGNNCC 6 cut(s) 241, 305, 885, 1108, 1294, 1920
BmrFI CCNGG 6 cut(s) 762, 888, 1104, 1153, 1517, 2921
BmrI ACTGGG 1 cut(s) 1330
BmsI GCATC 8 cut(s) 258, 262, 512, 1325, 1771, 2332, 2906, 2974
BmuI ACTGGG 1 cut(s) 1330
BpiI GAAGAC 3 cut(s) 131, 259, 551
BpmI CTGGAG 1 cut(s) 1143
Bpu10I CCTNAGC 3 cut(s) 104, 1941, 2469
BpuEI CTTGAG 1 cut(s) 2369
BpuMI CCSGG 1 cut(s) 888
BsaAI YACGTR 1 cut(s) 76
BsaI GGTCTC 3 cut(s) 481, 2067, 2833
BsaJI CCNNGG 4 cut(s) 672, 887, 1606, 2684
BsaWI WCCGGW 1 cut(s) 2107
BsaXI ACNNNNNCTCC 4 cut(s) 759, 789, 1521, 1551
Bsc4I CCNNNNNNNGG 9 cut(s) 142, 143, 144, 208, 1065, 1928, 2165, 2248, 2890
Bse1I ACTGG 4 cut(s) 1271, 1336, 1951, 2398
Bse3DI GCAATG 4 cut(s) 899, 1714, 1791, 2005
BseBI CCWGG 5 cut(s) 762, 1104, 1153, 1517, 2921
BseDI CCNNGG 4 cut(s) 672, 887, 1606, 2684
BseGI GGATG 6 cut(s) 249, 1164, 1795, 1816, 2347, 2921
BseLI CCNNNNNNNGG 9 cut(s) 142, 143, 144, 208, 1065, 1928, 2165, 2248, 2890
BseMI GCAATG 4 cut(s) 899, 1714, 1791, 2005
BseNI ACTGG 4 cut(s) 1271, 1336, 1951, 2398
BseRI GAGGAG 4 cut(s) 595, 780, 2055, 2775
BseXI GCAGC 7 cut(s) 97, 628, 678, 937, 1101, 1670, 2591
BseYI CCCAGC 3 cut(s) 88, 1439, 1540
BsgI GTGCAG 1 cut(s) 104
BshFI GGCC 5 cut(s) 1107, 1568, 1863, 2169, 2544
BshNI GGYRCC 1 cut(s) 303
BsiHKAI GWGCWC 4 cut(s) 189, 1178, 1210, 1576
BsiHKCI CYCGRG 4 cut(s) 30, 671, 1109, 2830
BsiSI CCGG 2 cut(s) 887, 2108
BslFI GGGAC 2 cut(s) 271, 2734
BslI CCNNNNNNNGG 9 cut(s) 142, 143, 144, 208, 1065, 1928, 2165, 2248, 2890
BsmAI GTCTC 4 cut(s) 481, 1260, 2067, 2833
BsmFI GGGAC 2 cut(s) 271, 2734
BsmI GAATGC 1 cut(s) 2081
BsnI GGCC 5 cut(s) 1107, 1568, 1863, 2169, 2544
Bso31I GGTCTC 3 cut(s) 481, 2067, 2833
BsoBI CYCGRG 4 cut(s) 30, 671, 1109, 2830
Bsp1286I GDGCHC 5 cut(s) 31, 189, 1178, 1210, 1576
Bsp1407I TGTACA 2 cut(s) 1417, 2818
Bsp19I CCATGG 1 cut(s) 2684
BspANI GGCC 5 cut(s) 1107, 1568, 1863, 2169, 2544
BspHI TCATGA 1 cut(s) 2313
BspLI GGNNCC 6 cut(s) 241, 305, 885, 1108, 1294, 1920
BspMAI CTGCAG 3 cut(s) 1173, 2166, 2581
BspT107I GGYRCC 1 cut(s) 303
BspTI CTTAAG 1 cut(s) 2993
BspTNI GGTCTC 3 cut(s) 481, 2067, 2833
BsrDI GCAATG 4 cut(s) 899, 1714, 1791, 2005
BsrGI TGTACA 2 cut(s) 1417, 2818
BsrI ACTGG 4 cut(s) 1271, 1336, 1951, 2398
BssECI CCNNGG 4 cut(s) 672, 887, 1606, 2684
BssNAI GTATAC 1 cut(s) 2932
BssSI CACGAG 1 cut(s) 726
BssT1I CCWWGG 2 cut(s) 1606, 2684
Bst1107I GTATAC 1 cut(s) 2932
Bst2BI CACGAG 1 cut(s) 726
Bst2UI CCWGG 5 cut(s) 762, 1104, 1153, 1517, 2921
Bst4CI ACNGT 7 cut(s) 723, 786, 1362, 1630, 2056, 2094, 2639
Bst6I CTCTTC 4 cut(s) 144, 691, 1929, 2517
BstAFI CTTAAG 1 cut(s) 2993
BstAUI TGTACA 2 cut(s) 1417, 2818
BstBAI YACGTR 1 cut(s) 76
BstC8I GCNNGC 8 cut(s) 277, 1036, 1326, 1353, 1431, 1566, 1948, 2381
BstDSI CCRYGG 1 cut(s) 2684
BstENI CCTNNNNNAGG 3 cut(s) 206, 1926, 2246
BstF5I GGATG 6 cut(s) 249, 1164, 1795, 1816, 2347, 2921
BstHHI GCGC 1 cut(s) 865
BstMAI GTCTC 4 cut(s) 481, 1260, 2067, 2833
BstMWI GCNNNNNNNGC 5 cut(s) 94, 246, 918, 1031, 1477
BstNI CCWGG 5 cut(s) 762, 1104, 1153, 1517, 2921
BstNSI RCATGY 2 cut(s) 1950, 2563
BstSCI CCNGG 6 cut(s) 760, 886, 1102, 1151, 1515, 2919
BstSFI CTRYAG 4 cut(s) 873, 1169, 2162, 2577
BstV1I GCAGC 7 cut(s) 97, 628, 678, 937, 1101, 1670, 2591
BstV2I GAAGAC 3 cut(s) 131, 259, 551
BstX2I RGATCY 6 cut(s) 34, 49, 883, 1285, 1292, 2511
BstXI CCANNNNNNTGG 1 cut(s) 1613
BstYI RGATCY 6 cut(s) 34, 49, 883, 1285, 1292, 2511
BstZ17I GTATAC 1 cut(s) 2932
BsuRI GGCC 5 cut(s) 1107, 1568, 1863, 2169, 2544
BtgI CCRYGG 1 cut(s) 2684
BtgZI GCGATG 2 cut(s) 59, 2718
BtrI CACGTC 1 cut(s) 1139
BtsCI GGATG 6 cut(s) 249, 1164, 1795, 1816, 2347, 2921
BtsI GCAGTG 2 cut(s) 1319, 1591
BtsIMutI CAGTG 4 cut(s) 457, 1319, 1591, 3034
Cac8I GCNNGC 8 cut(s) 277, 1036, 1326, 1353, 1431, 1566, 1948, 2381
CciI TCATGA 1 cut(s) 2313
CfoI GCGC 1 cut(s) 865
Cfr13I GGNCC 9 cut(s) 108, 619, 988, 1106, 1835, 1861, 2167, 2542, 2635
CseI GACGC 1 cut(s) 910
Csp6I GTAC 8 cut(s) 217, 787, 1358, 1418, 2273, 2775, 2819, 2846
CviQI GTAC 8 cut(s) 217, 787, 1358, 1418, 2273, 2775, 2819, 2846
DraI TTTAAA 1 cut(s) 1221
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
Eam1104I CTCTTC 4 cut(s) 144, 691, 1929, 2517
EarI CTCTTC 4 cut(s) 144, 691, 1929, 2517
EciI GGCGGA 3 cut(s) 1081, 1171, 2637
Ecl136II GAGCTC 2 cut(s) 187, 1208
Eco130I CCWWGG 2 cut(s) 1606, 2684
Eco24I GRGCYC 3 cut(s) 31, 189, 1210
Eco31I GGTCTC 3 cut(s) 481, 2067, 2833
Eco47I GGWCC 5 cut(s) 108, 619, 988, 1835, 2635
Eco53kI GAGCTC 2 cut(s) 187, 1208
Eco57I CTGAAG 6 cut(s) 46, 145, 657, 2541, 3010, 3035
Eco88I CYCGRG 4 cut(s) 30, 671, 1109, 2830
EcoICRI GAGCTC 2 cut(s) 187, 1208
EcoNI CCTNNNNNAGG 3 cut(s) 206, 1926, 2246
EcoRI GAATTC 2 cut(s) 1496, 3044
EcoRII CCWGG 5 cut(s) 760, 1102, 1151, 1515, 2919
EcoT14I CCWWGG 2 cut(s) 1606, 2684
EcoT38I GRGCYC 3 cut(s) 31, 189, 1210
ErhI CCWWGG 2 cut(s) 1606, 2684
FalI AAGNNNNNCTT 4 cut(s) 113, 145, 3008, 3040
FaqI GGGAC 2 cut(s) 271, 2734
FauI CCCGC 1 cut(s) 284
FauNDI CATATG 1 cut(s) 1690
FbaI TGATCA 1 cut(s) 1588
FblI GTMKAC 2 cut(s) 168, 2931
FokI GGATG 6 cut(s) 236, 1171, 1802, 1823, 2354, 2928
FriOI GRGCYC 3 cut(s) 31, 189, 1210
FspBI CTAG 4 cut(s) 609, 1719, 2454, 2772
GlaI GCGC 1 cut(s) 864
GsaI CCCAGC 3 cut(s) 92, 1443, 1544
GsuI CTGGAG 1 cut(s) 1143
HaeIII GGCC 5 cut(s) 1107, 1568, 1863, 2169, 2544
HapII CCGG 2 cut(s) 887, 2108
HgaI GACGC 1 cut(s) 910
HhaI GCGC 1 cut(s) 865
Hin6I GCGC 1 cut(s) 863
HinP1I GCGC 1 cut(s) 863
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HpaII CCGG 2 cut(s) 887, 2108
HphI GGTGA 8 cut(s) 148, 426, 428, 946, 1505, 2491, 2639, 2779
Hpy166II GTNNAC 7 cut(s) 156, 169, 397, 619, 1420, 1701, 2932
Hpy8I GTNNAC 7 cut(s) 156, 169, 397, 619, 1420, 1701, 2932
Hpy99I CGWCG 5 cut(s) 173, 185, 209, 230, 1013
HpyCH4III ACNGT 7 cut(s) 723, 786, 1362, 1630, 2056, 2094, 2639
HpyCH4IV ACGT 8 cut(s) 75, 789, 942, 995, 1138, 2743, 2874, 2988
HpyF10VI GCNNNNNNNGC 5 cut(s) 94, 246, 918, 1031, 1477
HpySE526I ACGT 8 cut(s) 75, 789, 942, 995, 1138, 2743, 2874, 2988
HspAI GCGC 1 cut(s) 863
Ksp22I TGATCA 1 cut(s) 1588
LmnI GCTCC 2 cut(s) 245, 1205
Lsp1109I GCAGC 7 cut(s) 97, 628, 678, 937, 1101, 1670, 2591
LweI GCATC 8 cut(s) 258, 262, 512, 1325, 1771, 2332, 2906, 2974
MaeI CTAG 4 cut(s) 609, 1719, 2454, 2772
MaeII ACGT 8 cut(s) 75, 789, 942, 995, 1138, 2743, 2874, 2988
MaeIII GTNAC 8 cut(s) 416, 723, 790, 934, 1649, 2138, 2219, 2767
MfeI CAATTG 1 cut(s) 1488
MflI RGATCY 6 cut(s) 34, 49, 883, 1285, 1292, 2511
MhlI GDGCHC 5 cut(s) 31, 189, 1178, 1210, 1576
MlyI GAGTC 4 cut(s) 392, 1605, 2311, 2473
MmeI TCCRAC 3 cut(s) 1437, 1788, 2303
MroXI GAANNNNTTC 1 cut(s) 3044
MslI CAYNNNNRTG 1 cut(s) 1181
MspA1I CMGCKG 2 cut(s) 88, 1661
MspCI CTTAAG 1 cut(s) 2993
MspI CCGG 2 cut(s) 887, 2108
MspR9I CCNGG 6 cut(s) 762, 888, 1104, 1153, 1517, 2921
MunI CAATTG 1 cut(s) 1488
Mva1269I GAATGC 1 cut(s) 2081
MvaI CCWGG 5 cut(s) 762, 1104, 1153, 1517, 2921
MwoI GCNNNNNNNGC 5 cut(s) 94, 246, 918, 1031, 1477
NciI CCSGG 1 cut(s) 888
NcoI CCATGG 1 cut(s) 2684
NdeI CATATG 1 cut(s) 1690
NlaIV GGNNCC 6 cut(s) 241, 305, 885, 1108, 1294, 1920
NmeAIII GCCGAG 2 cut(s) 380, 2941
NmuCI GTSAC 7 cut(s) 416, 723, 790, 934, 2138, 2219, 2767
NspI RCATGY 2 cut(s) 1950, 2563
PaeI GCATGC 1 cut(s) 1950
PaeR7I CTCGAG 1 cut(s) 2830
PagI TCATGA 1 cut(s) 2313
PcsI WCGNNNNNNNCGW 5 cut(s) 63, 177, 186, 231, 240
PctI GAATGC 1 cut(s) 2081
PdmI GAANNNNTTC 1 cut(s) 3044
PleI GAGTC 4 cut(s) 392, 1604, 2311, 2472
PpsI GAGTC 4 cut(s) 392, 1604, 2311, 2472
Ppu21I YACGTR 1 cut(s) 76
Psp124BI GAGCTC 2 cut(s) 189, 1210
Psp6I CCWGG 5 cut(s) 760, 1102, 1151, 1515, 2919
PspFI CCCAGC 3 cut(s) 88, 1439, 1540
PspGI CCWGG 5 cut(s) 760, 1102, 1151, 1515, 2919
PspN4I GGNNCC 6 cut(s) 241, 305, 885, 1108, 1294, 1920
PspPI GGNCC 9 cut(s) 108, 619, 988, 1106, 1835, 1861, 2167, 2542, 2635
PstI CTGCAG 3 cut(s) 1173, 2166, 2581
PsuI RGATCY 6 cut(s) 34, 49, 883, 1285, 1292, 2511
PvuII CAGCTG 2 cut(s) 88, 1661
RsaI GTAC 8 cut(s) 218, 788, 1359, 1419, 2274, 2776, 2820, 2847
RsaNI GTAC 8 cut(s) 217, 787, 1358, 1418, 2273, 2775, 2819, 2846
RseI CAYNNNNRTG 1 cut(s) 1181
SacI GAGCTC 2 cut(s) 189, 1210
SalI GTCGAC 1 cut(s) 167
Sau96I GGNCC 9 cut(s) 108, 619, 988, 1106, 1835, 1861, 2167, 2542, 2635
SbfI CCTGCAGG 1 cut(s) 2166
ScaI AGTACT 2 cut(s) 218, 2776
SchI GAGTC 4 cut(s) 392, 1605, 2311, 2473
ScrFI CCNGG 6 cut(s) 762, 888, 1104, 1153, 1517, 2921
SdaI CCTGCAGG 1 cut(s) 2166
SduI GDGCHC 5 cut(s) 31, 189, 1178, 1210, 1576
SfaNI GCATC 8 cut(s) 258, 262, 512, 1325, 1771, 2332, 2906, 2974
SfcI CTRYAG 4 cut(s) 873, 1169, 2162, 2577
Sfr274I CTCGAG 1 cut(s) 2830
SinI GGWCC 5 cut(s) 108, 619, 988, 1835, 2635
SlaI CTCGAG 1 cut(s) 2830
SmiMI CAYNNNNRTG 1 cut(s) 1181
SmlI CTYRAG 3 cut(s) 2384, 2830, 2993
SmoI CTYRAG 3 cut(s) 2384, 2830, 2993
SpeI ACTAGT 1 cut(s) 2771
SphI GCATGC 1 cut(s) 1950
Sse8387I CCTGCAGG 1 cut(s) 2166
SspI AATATT 1 cut(s) 1770
SspMI CTAG 4 cut(s) 609, 1719, 2454, 2772
SstI GAGCTC 2 cut(s) 189, 1210
StyD4I CCNGG 6 cut(s) 760, 886, 1102, 1151, 1515, 2919
StyI CCWWGG 2 cut(s) 1606, 2684
TaaI ACNGT 7 cut(s) 723, 786, 1362, 1630, 2056, 2094, 2639
TaiI ACGT 8 cut(s) 78, 792, 945, 998, 1141, 2746, 2877, 2991
TaqI TCGA 9 cut(s) 168, 204, 270, 1210, 1275, 1670, 2831, 2878, 2889
TatI WGTACW 5 cut(s) 216, 1357, 1417, 2774, 2818
TauI GCSGC 3 cut(s) 351, 2172, 2326
TscAI CASTG 4 cut(s) 457, 1326, 1591, 3034
TseFI GTSAC 7 cut(s) 416, 723, 790, 934, 2138, 2219, 2767
TseI GCWGC 7 cut(s) 85, 641, 666, 950, 1114, 1658, 2579
Tsp45I GTSAC 7 cut(s) 416, 723, 790, 934, 2138, 2219, 2767
TspGWI ACGGA 3 cut(s) 232, 1094, 2578
TspRI CASTG 4 cut(s) 457, 1326, 1591, 3034
Vha464I CTTAAG 1 cut(s) 2993
VpaK11BI GGWCC 5 cut(s) 108, 619, 988, 1835, 2635
XagI CCTNNNNNAGG 3 cut(s) 206, 1926, 2246
XapI RAATTY 8 cut(s) 1162, 1496, 1971, 2074, 2114, 2209, 2599, 3044
XceI RCATGY 2 cut(s) 1950, 2563
XcmI CCANNNNNNNNNTGG 1 cut(s) 2093
XhoI CTCGAG 1 cut(s) 2830
XmiI GTMKAC 2 cut(s) 168, 2931
XmnI GAANNNNTTC 1 cut(s) 3044
XspI CTAG 4 cut(s) 609, 1719, 2454, 2772
ZrmI AGTACT 2 cut(s) 218, 2776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.