Rw5G000280

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006300
Physical Location & Seq
Reverse (-)
238634 .. 244063
5430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000280.1

Sequence Viewer

Length: 2985 bp
ATGAAGCTGGTGGTTCAAGTGATGGGGGCTCGAGATCTGCCGGCAATGGATCTAAACGGGCTGAGTGATCCGTACGTGAAGGTGAAGATGGGAAAGCAGAAGTTCAAGACCAAAGTGGTGAAGAAGACGTTAAACCCATATTGGGGTGAAGAGTTCACGTTTCGAGTCGACGACCTCAACGACGAGCTCCTCATCTCTTGTTTGGATGAAGATAAGTACTTCAACGACGACTTCGTGGGCTGCGTCAAATTCCCTGTTTCCCAAGTCTTCGATGCCCCCAACAAGTGCCTCGACACTTGTTGGCACCCTCTCCAGCCCAAGAGCAAGAAGCCCAAGCACAAGGATTGCGGCGAGATTCTTCTTAATATCTCTCTCAGTACAAACAATGCATTCTCCGACTCCGCTTCCGATGGTGATCACATCAGGAGGGATTCCGACATTGGAGGTGAATCACCCTCAAGGTTATCCGAAACGGCTTCGGCTTCCCCTCAGAGAGGCAAGCTTGATGAACTTCCATTCTCCAAGGAAGATAAAGAAAAGGAAAAGACTCTTGCCCAGAAATCCCTTGCTGGCCGACTTGTTCAGATGTTCAATAAAAATCCTGACGCACCTCCAATATCTTCAAACCACTCTAGCAAAGCTGACTTGACTGAGCTTGTTGAAACCAAGGCCGACATTTCTGAGGCCACTTATTCTGAGGATCACTCCTCTTGTAACCTACCGGGTGGAGTGCTTCTAGATCAAATGTATGTTACTGAACCCAAACACCTCAACACTTTACTCTTTTCACCGGATTCTACTTTTCCAAAGGCGTTGGCAGACCTGCATGGAACTACAGAATTGGAACAAGGACCCTGGAAATTTGAAAACGACAGCTTAAAAAGAGTGGTTACATATGTTAAGGCAGCTAGCAAATTAATCAAGGCTTGCAAAGGAAGTGAGGACCAAACATATCTCAAAGCCGATGGGAAAGTTTTTGCAGTTTTTTCAAGTGTAAGCACTCCTGATGTTCCATATGGGAAAACTTTTAGGACCGAGTTGCTCTACTGCATTACACCTGGGCCTGAGCTGCCATCGGGAGAACAATCTACACGCTTTGTAATATCCTGGCGCATGAATTTTCTGCAGAGCACCATGATGAAAGGAATGATAGAAAATGGAGCTCGGCAAGGTTTAAGGACAAACGTTAAGCCAGCTGATTCCAAAGACCTTGGATCCAATAAGGACCAGGTTTTGGCATCATTGCAGGCTGAATCCCAGTCTGATTGGAAGCTGGCAGTACAATATTTTGCTAATTTCACTGTGGTCTCCACATTTTTTATTGGATTGTATATGCTGGTACACATCTACCTGGCTACACCCAGCACTATTCAAGGGTTGGAGTTTGTTGGGCTTGACTTGCCAGATTCAATTGGTGAATTCATTGTTTGTGGTGTCCTGGTACTTCAAGGAGAACGTGTGCTGGGTTTGATCTCACGCTTTATGCAGGCCAGAGTGCAGAAAGGCAGTGACCATGGAGTCAAAGCCCGAGGAGATGGTTGGTTGCTAACTGTTGCCTTGATTGAAGGAAGTAATATAGCAGCTGTTGATTCAAGTGGGTTCTCTGATCCATACGTGGTGTTTTCTTGCAATGGAAAAGCTAGAACTAGCTCAATCAAGTTCCAGAAATGCGACCCTCTGTGGAATGAAATATTTGAGTTTGATGCAATGGATGAGCCTCCTTCTGTGCTGGATGTGGAAATTTATGATTTTGATGGTCCTTTTGATGAAGCTACCTCTCTGGGCCATGCTGAAATCAATTTTGTGAAAACCAATATATCAGATTTAGCTGATATGTGGATTCCTCTTCAAGGAAAGTTAGCTCAGGCTTGTCAGTCAAAACTCCACTTGAGAATTTTCTTAAACAATACAAGAGGTGGCAATGTTGTTAATCACTTTTTAAATAAGATGGAGAAGGAGGTTGGAAAGAAGATTACTGTACGTTCTCCTCAAACAAATTCAGCATTCCAAAAACTCTTTGGGCTTCCACCAGAGGAATTTCTTATCAACGACTTTACATGTCACTTGAAACGAAAAATGCCCCTGCAGGGCCGCCTATTTCTTTCTGCGAGAATAATTGGGTTCCATGCAAATTTATTTGGTCGCAAGACAAAGTTCTTTTTCCTCTGGGAGGATATAGAAGATATTCATATTGTTCCTCCAACTCTGTCATCTATGGGTAGTCCAACTATAGTCATGACTCTGCGGCAAGGTAGAGGTATGGATGCAAGGCATGGTGCAAAGACACAGGATGAGGAAGGCAGGCTGAAGTTCCACTTCCAGTCCTTCGTATCCTTCAATGTAGCGAACAGGACAATTATGGCCCTGTGGAAGGCTAGATCTTTGAGTCCTGAGCAGAAGGTGCAAATAATAGAAGAAGAATCAGAAGTCAAGAGCCTCCAAACTGAAGAGAGTGGGTCTTTCTTGGGCCTTGATGATGTCAGCATGTCTGAGGTTTATTCATCCTTGCATTCAGTTCCTACAAATTTCTTCGCAGAGCTATTTGGTGGAGGTGAATTGGATCGTAGAGTTATGGAGAAAGCTGGTTGTCTTAACTATTCTCACACCCCATGGGAATCAGAGAAGGGCGATGTCTATGTGAGGCAAATATATTACAGATATGACAAACGTGTTTCCCAATATAGAGGAGAGGTGACTAGTACTCAGCAAAAATCCCGCCTTTCCGATAGAAATGGTTGGCTTTTTCAAGAAGTCATGACCCTCCATGCAATTCCACTTGGTGACTATTTCAATGTTCACATCCGATACCAAATTGAGGACTCGTCCTCAACACCACCAGGGTGTCTGGTAAAAGTATACTTTGGCATTGAGTGGCTCAAAAGCACTAAGCATCAGAAAAGAATTACAAAAAATGTTCTCAAGAATCTGCAAGATCGCCTAAAGGTCTCCTTTGCTGTAGTTGAGAAGGAGTTCACATCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

994

Amino Acids

111.72

Weight (kDa)

6.38

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 96 2.3e-29 C2 domain
VASt PF16016 245 - 391 4.3e-32 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 515 - 616 6.4e-16 C2 domain
GRAM PF02893 668 - 775 3.1e-17 GRAM domain
VASt PF16016 840 - 974 7.3e-23 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1517
Acc36I ACCTGC 1 cut(s) 831
AccB1I GGYRCC 1 cut(s) 303
AccB7I CCANNNNNTGG 1 cut(s) 1234
AccI GTMKAC 2 cut(s) 168, 2856
AciI CCGC 5 cut(s) 348, 402, 2092, 2245, 2716
AclI AACGTT 1 cut(s) 1185
AclWI GGATC 7 cut(s) 57, 62, 708, 1209, 1222, 1601, 2568
AcoI YGGCCR 1 cut(s) 571
AcuI CTGAAG 2 cut(s) 2327, 2466
AdeI CACNNNGTG 1 cut(s) 2780
AfaI GTAC 8 cut(s) 74, 218, 379, 1281, 1341, 1443, 1980, 2701
AfiI CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1234, 1850, 2087, 2170, 2371, 2815
AflIII ACRYGT 3 cut(s) 1456, 2057, 2668
AhlI ACTAGT 1 cut(s) 2696
AjnI CCWGG 7 cut(s) 854, 1057, 1106, 1227, 1350, 1437, 2836
AleI CACNNNNGTG 1 cut(s) 2839
Alw21I GWGCWC 3 cut(s) 189, 1133, 1165
Alw26I GTCTC 2 cut(s) 1312, 2950
AlwI GGATC 7 cut(s) 57, 62, 708, 1209, 1222, 1601, 2568
Ama87I CYCGRG 2 cut(s) 30, 1527
AoxI GGCC 9 cut(s) 571, 669, 684, 1061, 1488, 1783, 2089, 2361, 2467
ApeKI GCWGC 4 cut(s) 240, 905, 1069, 1580
AseI ATTAAT 1 cut(s) 917
Asp700I GAANNNNTTC 2 cut(s) 2184, 2969
AspLEI GCGC 1 cut(s) 1113
AsuC2I CCSGG 1 cut(s) 723
AsuNHI GCTAGC 1 cut(s) 908
AvaI CYCGRG 2 cut(s) 30, 1527
AvaII GGWCC 5 cut(s) 851, 943, 1032, 1225, 1757
BamHI GGATCC 1 cut(s) 1214
BanI GGYRCC 1 cut(s) 303
BanII GRGCYC 3 cut(s) 31, 189, 1165
BbsI GAAGAC 2 cut(s) 131, 259
Bbv12I GWGCWC 3 cut(s) 189, 1133, 1165
BbvI GCAGC 4 cut(s) 227, 917, 1056, 1592
BccI CCATC 8 cut(s) 16, 82, 404, 959, 1081, 1529, 1748, 1942
BceAI ACGGC 1 cut(s) 489
BciT130I CCWGG 7 cut(s) 856, 1059, 1108, 1229, 1352, 1439, 2838
BciVI GTATCC 1 cut(s) 2341
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 1 cut(s) 723
BcoDI GTCTC 2 cut(s) 1312, 2950
BcuI ACTAGT 1 cut(s) 2696
BfaI CTAG 7 cut(s) 633, 737, 909, 1641, 1647, 2376, 2697
BfmI CTRYAG 5 cut(s) 834, 1124, 2084, 2229, 2955
BfuAI ACCTGC 1 cut(s) 831
BfuI GTATCC 1 cut(s) 2341
BglII AGATCT 2 cut(s) 34, 2378
BisI GCNGC 7 cut(s) 241, 349, 906, 1070, 1581, 2092, 2246
BlsI GCNGC 7 cut(s) 242, 350, 907, 1071, 1582, 2093, 2247
BmcAI AGTACT 2 cut(s) 218, 2701
Bme1390I CCNGG 8 cut(s) 723, 856, 1059, 1108, 1229, 1352, 1439, 2838
Bme18I GGWCC 5 cut(s) 851, 943, 1032, 1225, 1757
BmeT110I CYCGRG 2 cut(s) 30, 1527
BmiI GGNNCC 4 cut(s) 305, 853, 1216, 2123
BmrFI CCNGG 8 cut(s) 723, 856, 1059, 1108, 1229, 1352, 1439, 2838
BmrI ACTGGG 1 cut(s) 1252
BmsI GCATC 5 cut(s) 262, 1247, 1693, 2254, 2899
BmtI GCTAGC 1 cut(s) 912
BmuI ACTGGG 1 cut(s) 1252
BpiI GAAGAC 2 cut(s) 131, 259
BplI GAGNNNNNCTC 2 cut(s) 689, 721
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 3 cut(s) 1065, 1863, 2391
BpuEI CTTGAG 3 cut(s) 442, 1909, 2903
BpuMI CCSGG 1 cut(s) 723
BsaAI YACGTR 2 cut(s) 76, 1615
BsaBI GATNNNNATC 1 cut(s) 414
BsaI GGTCTC 2 cut(s) 1312, 2950
BsaJI CCNNGG 9 cut(s) 522, 666, 854, 1058, 1210, 1513, 1528, 2609, 2837
BsaWI WCCGGW 1 cut(s) 790
BsaXI ACNNNNNCTCC 2 cut(s) 1443, 1473
Bsc4I CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1234, 1850, 2087, 2170, 2371, 2815
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 1258, 2320
Bse3DI GCAATG 5 cut(s) 51, 1241, 1636, 1713, 1927
Bse8I GATNNNNATC 1 cut(s) 414
BseBI CCWGG 7 cut(s) 856, 1059, 1108, 1229, 1352, 1439, 2838
BseDI CCNNGG 9 cut(s) 522, 666, 854, 1058, 1210, 1513, 1528, 2609, 2837
BseGI GGATG 7 cut(s) 211, 1717, 1738, 2269, 2296, 2501, 2799
BseJI GATNNNNATC 1 cut(s) 414
BseLI CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1234, 1850, 2087, 2170, 2371, 2815
BseMI GCAATG 5 cut(s) 51, 1241, 1636, 1713, 1927
BseNI ACTGG 2 cut(s) 1258, 2320
BseRI GAGGAG 5 cut(s) 179, 697, 1545, 1977, 2700
BseXI GCAGC 4 cut(s) 227, 917, 1056, 1592
BseYI CCCAGC 2 cut(s) 1361, 1462
BsgI GTGCAG 1 cut(s) 1517
BshFI GGCC 9 cut(s) 573, 671, 686, 1063, 1490, 1785, 2091, 2363, 2469
BshNI GGYRCC 1 cut(s) 303
BsiHKAI GWGCWC 3 cut(s) 189, 1133, 1165
BsiHKCI CYCGRG 2 cut(s) 30, 1527
BsiSI CCGG 3 cut(s) 41, 722, 791
BsiWI CGTACG 1 cut(s) 72
BslI CCNNNNNNNGG 9 cut(s) 142, 143, 494, 1234, 1850, 2087, 2170, 2371, 2815
BsmAI GTCTC 2 cut(s) 1312, 2950
BsmI GAATGC 3 cut(s) 389, 2003, 2509
BsnI GGCC 9 cut(s) 573, 671, 686, 1063, 1490, 1785, 2091, 2363, 2469
Bso31I GGTCTC 2 cut(s) 1312, 2950
BsoBI CYCGRG 2 cut(s) 30, 1527
Bsp1286I GDGCHC 4 cut(s) 31, 189, 1133, 1165
Bsp19I CCATGG 2 cut(s) 1513, 2609
BspACI CCGC 5 cut(s) 348, 402, 2092, 2245, 2716
BspANI GGCC 9 cut(s) 573, 671, 686, 1063, 1490, 1785, 2091, 2363, 2469
BspHI TCATGA 2 cut(s) 2235, 2754
BspLI GGNNCC 4 cut(s) 305, 853, 1216, 2123
BspMAI CTGCAG 2 cut(s) 1128, 2088
BspMI ACCTGC 1 cut(s) 831
BspOI GCTAGC 1 cut(s) 912
BspPI GGATC 7 cut(s) 57, 62, 708, 1209, 1222, 1601, 2568
BspT107I GGYRCC 1 cut(s) 303
BspTNI GGTCTC 2 cut(s) 1312, 2950
BsrDI GCAATG 5 cut(s) 51, 1241, 1636, 1713, 1927
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 2 cut(s) 1258, 2320
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 9 cut(s) 522, 666, 854, 1058, 1210, 1513, 1528, 2609, 2837
BssNAI GTATAC 1 cut(s) 2857
BssT1I CCWWGG 5 cut(s) 522, 666, 1210, 1513, 2609
Bst1107I GTATAC 1 cut(s) 2857
Bst2UI CCWGG 7 cut(s) 856, 1059, 1108, 1229, 1352, 1439, 2838
Bst4CI ACNGT 3 cut(s) 1303, 1552, 1978
Bst6I CTCTTC 3 cut(s) 144, 1851, 2442
BstAPI GCANNNNNTGC 1 cut(s) 2401
BstBAI YACGTR 2 cut(s) 76, 1615
BstDSI CCRYGG 2 cut(s) 1513, 2609
BstENI CCTNNNNNAGG 4 cut(s) 492, 1848, 2168, 2369
BstF5I GGATG 7 cut(s) 211, 1717, 1738, 2269, 2296, 2501, 2799
BstHHI GCGC 1 cut(s) 1113
BstMAI GTCTC 2 cut(s) 1312, 2950
BstMWI GCNNNNNNNGC 3 cut(s) 1069, 1399, 2401
BstNI CCWGG 7 cut(s) 856, 1059, 1108, 1229, 1352, 1439, 2838
BstNSI RCATGY 2 cut(s) 2061, 2488
BstSCI CCNGG 8 cut(s) 721, 854, 1057, 1106, 1227, 1350, 1437, 2836
BstSFI CTRYAG 5 cut(s) 834, 1124, 2084, 2229, 2955
BstV1I GCAGC 4 cut(s) 227, 917, 1056, 1592
BstV2I GAAGAC 2 cut(s) 131, 259
BstX2I RGATCY 4 cut(s) 34, 49, 1214, 2378
BstYI RGATCY 4 cut(s) 34, 49, 1214, 2378
BstZ17I GTATAC 1 cut(s) 2857
BsuI GTATCC 1 cut(s) 2341
BsuRI GGCC 9 cut(s) 573, 671, 686, 1063, 1490, 1785, 2091, 2363, 2469
BtgI CCRYGG 2 cut(s) 1513, 2609
BtgZI GCGATG 1 cut(s) 2643
BtsCI GGATG 7 cut(s) 211, 1717, 1738, 2269, 2296, 2501, 2799
BtsI GCAGTG 1 cut(s) 1513
BtsIMutI CAGTG 2 cut(s) 1299, 1513
BveI ACCTGC 1 cut(s) 831
CciI TCATGA 2 cut(s) 2235, 2754
CfoI GCGC 1 cut(s) 1113
Cfr10I RCCGGY 1 cut(s) 40
CseI GACGC 2 cut(s) 232, 614
CsiI ACCWGGT 1 cut(s) 1227
Csp6I GTAC 8 cut(s) 73, 217, 378, 1280, 1340, 1442, 1979, 2700
CviQI GTAC 8 cut(s) 73, 217, 378, 1280, 1340, 1442, 1979, 2700
DraI TTTAAA 1 cut(s) 1941
DraIII CACNNNGTG 1 cut(s) 2780
DrdI GACNNNNNNGTC 1 cut(s) 1517
DseDI GACNNNNNNGTC 1 cut(s) 1517
EaeI YGGCCR 1 cut(s) 571
Eam1104I CTCTTC 3 cut(s) 144, 1851, 2442
EarI CTCTTC 3 cut(s) 144, 1851, 2442
Ecl136II GAGCTC 2 cut(s) 187, 1163
Eco130I CCWWGG 5 cut(s) 522, 666, 1210, 1513, 2609
Eco24I GRGCYC 3 cut(s) 31, 189, 1165
Eco31I GGTCTC 2 cut(s) 1312, 2950
Eco47I GGWCC 5 cut(s) 851, 943, 1032, 1225, 1757
Eco53kI GAGCTC 2 cut(s) 187, 1163
Eco57I CTGAAG 2 cut(s) 2327, 2466
Eco88I CYCGRG 2 cut(s) 30, 1527
EcoICRI GAGCTC 2 cut(s) 187, 1163
EcoNI CCTNNNNNAGG 4 cut(s) 492, 1848, 2168, 2369
EcoO109I RGGNCCY 1 cut(s) 851
EcoRI GAATTC 1 cut(s) 1418
EcoRII CCWGG 7 cut(s) 854, 1057, 1106, 1227, 1350, 1437, 2836
EcoT14I CCWWGG 5 cut(s) 522, 666, 1210, 1513, 2609
EcoT22I ATGCAT 1 cut(s) 391
EcoT38I GRGCYC 3 cut(s) 31, 189, 1165
ErhI CCWWGG 5 cut(s) 522, 666, 1210, 1513, 2609
FalI AAGNNNNNCTT 4 cut(s) 2300, 2332, 2933, 2965
FauI CCCGC 1 cut(s) 2723
FauNDI CATATG 2 cut(s) 895, 1015
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 2 cut(s) 168, 2856
Fnu4HI GCNGC 7 cut(s) 241, 349, 906, 1070, 1581, 2092, 2246
FokI GGATG 7 cut(s) 218, 1724, 1745, 2276, 2303, 2488, 2786
FriOI GRGCYC 3 cut(s) 31, 189, 1165
Fsp4HI GCNGC 7 cut(s) 241, 349, 906, 1070, 1581, 2092, 2246
FspBI CTAG 7 cut(s) 633, 737, 909, 1641, 1647, 2376, 2697
GlaI GCGC 1 cut(s) 1112
GluI GCNGC 7 cut(s) 241, 349, 906, 1070, 1581, 2092, 2246
GsaI CCCAGC 2 cut(s) 1365, 1466
GsuI CTGGAG 1 cut(s) 296
HaeIII GGCC 9 cut(s) 573, 671, 686, 1063, 1490, 1785, 2091, 2363, 2469
HapII CCGG 3 cut(s) 41, 722, 791
HgaI GACGC 2 cut(s) 232, 614
HhaI GCGC 1 cut(s) 1113
Hin6I GCGC 1 cut(s) 1111
HinP1I GCGC 1 cut(s) 1111
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 500
HpaII CCGG 3 cut(s) 41, 722, 791
Hpy166II GTNNAC 6 cut(s) 156, 169, 1342, 2797, 2857, 2973
Hpy8I GTNNAC 6 cut(s) 156, 169, 1342, 2797, 2857, 2973
Hpy99I CGWCG 3 cut(s) 173, 185, 230
HpyCH4III ACNGT 3 cut(s) 1303, 1552, 1978
HpyCH4IV ACGT 8 cut(s) 75, 128, 158, 1185, 1456, 1614, 1981, 2668
HpyF10VI GCNNNNNNNGC 3 cut(s) 1069, 1399, 2401
HpySE526I ACGT 8 cut(s) 75, 128, 158, 1185, 1456, 1614, 1981, 2668
HspAI GCGC 1 cut(s) 1111
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Ksp22I TGATCA 1 cut(s) 415
LmnI GCTCC 2 cut(s) 192, 1160
Lsp1109I GCAGC 4 cut(s) 227, 917, 1056, 1592
LweI GCATC 5 cut(s) 262, 1247, 1693, 2254, 2899
MabI ACCWGGT 1 cut(s) 1227
MaeI CTAG 7 cut(s) 633, 737, 909, 1641, 1647, 2376, 2697
MaeII ACGT 8 cut(s) 75, 128, 158, 1185, 1456, 1614, 1981, 2668
MaeIII GTNAC 7 cut(s) 713, 751, 889, 1508, 2060, 2692, 2780
MfeI CAATTG 1 cut(s) 1410
MflI RGATCY 4 cut(s) 34, 49, 1214, 2378
MhlI GDGCHC 4 cut(s) 31, 189, 1133, 1165
MlyI GAGTC 7 cut(s) 174, 392, 541, 1527, 2233, 2395, 2813
MmeI TCCRAC 6 cut(s) 420, 459, 1359, 1942, 2225, 2249
Mph1103I ATGCAT 1 cut(s) 391
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 2 cut(s) 2184, 2969
MslI CAYNNNNRTG 2 cut(s) 1136, 2839
MspA1I CMGCKG 2 cut(s) 1196, 1583
MspI CCGG 3 cut(s) 41, 722, 791
MspR9I CCNGG 8 cut(s) 723, 856, 1059, 1108, 1229, 1352, 1439, 2838
MunI CAATTG 1 cut(s) 1410
Mva1269I GAATGC 3 cut(s) 389, 2003, 2509
MvaI CCWGG 7 cut(s) 856, 1059, 1108, 1229, 1352, 1439, 2838
MwoI GCNNNNNNNGC 3 cut(s) 1069, 1399, 2401
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 723
NcoI CCATGG 2 cut(s) 1513, 2609
NdeI CATATG 2 cut(s) 895, 1015
NgoMIV GCCGGC 1 cut(s) 40
NheI GCTAGC 1 cut(s) 908
NlaIV GGNNCC 4 cut(s) 305, 853, 1216, 2123
NmeAIII GCCGAG 1 cut(s) 1144
NmuCI GTSAC 4 cut(s) 1508, 2060, 2692, 2780
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 2 cut(s) 2061, 2488
OliI CACNNNNGTG 1 cut(s) 2839
PaeR7I CTCGAG 1 cut(s) 30
PagI TCATGA 2 cut(s) 2235, 2754
PciI ACATGT 1 cut(s) 2057
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 231, 240
PctI GAATGC 3 cut(s) 389, 2003, 2509
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 2 cut(s) 2184, 2969
Pfl23II CGTACG 1 cut(s) 72
PflMI CCANNNNNTGG 1 cut(s) 1234
PkrI GCNGC 7 cut(s) 242, 350, 907, 1071, 1582, 2093, 2247
PleI GAGTC 7 cut(s) 173, 392, 541, 1526, 2233, 2394, 2813
PpsI GAGTC 7 cut(s) 173, 392, 541, 1526, 2233, 2394, 2813
Ppu21I YACGTR 2 cut(s) 76, 1615
PpuMI RGGWCCY 1 cut(s) 851
PscI ACATGT 1 cut(s) 2057
PshBI ATTAAT 1 cut(s) 917
Psp124BI GAGCTC 2 cut(s) 189, 1165
Psp1406I AACGTT 1 cut(s) 1185
Psp5II RGGWCCY 1 cut(s) 851
Psp6I CCWGG 7 cut(s) 854, 1057, 1106, 1227, 1350, 1437, 2836
PspFI CCCAGC 2 cut(s) 1361, 1462
PspGI CCWGG 7 cut(s) 854, 1057, 1106, 1227, 1350, 1437, 2836
PspLI CGTACG 1 cut(s) 72
PspN4I GGNNCC 4 cut(s) 305, 853, 1216, 2123
PspPPI RGGWCCY 1 cut(s) 851
PstI CTGCAG 2 cut(s) 1128, 2088
PsuI RGATCY 4 cut(s) 34, 49, 1214, 2378
PvuII CAGCTG 2 cut(s) 1196, 1583
RsaI GTAC 8 cut(s) 74, 218, 379, 1281, 1341, 1443, 1980, 2701
RsaNI GTAC 8 cut(s) 73, 217, 378, 1280, 1340, 1442, 1979, 2700
RseI CAYNNNNRTG 2 cut(s) 1136, 2839
SacI GAGCTC 2 cut(s) 189, 1165
SalI GTCGAC 1 cut(s) 167
SatI GCNGC 7 cut(s) 241, 349, 906, 1070, 1581, 2092, 2246
SbfI CCTGCAGG 1 cut(s) 2088
ScaI AGTACT 2 cut(s) 218, 2701
SchI GAGTC 7 cut(s) 174, 392, 541, 1527, 2233, 2395, 2813
ScrFI CCNGG 8 cut(s) 723, 856, 1059, 1108, 1229, 1352, 1439, 2838
SdaI CCTGCAGG 1 cut(s) 2088
SduI GDGCHC 4 cut(s) 31, 189, 1133, 1165
SexAI ACCWGGT 1 cut(s) 1227
SfaNI GCATC 5 cut(s) 262, 1247, 1693, 2254, 2899
SfcI CTRYAG 5 cut(s) 834, 1124, 2084, 2229, 2955
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 5 cut(s) 851, 943, 1032, 1225, 1757
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 2 cut(s) 1136, 2839
SmlI CTYRAG 4 cut(s) 30, 457, 1888, 2918
SmoI CTYRAG 4 cut(s) 30, 457, 1888, 2918
SpeI ACTAGT 1 cut(s) 2696
Sse8387I CCTGCAGG 1 cut(s) 2088
SsiI CCGC 5 cut(s) 348, 402, 2092, 2245, 2716
SspI AATATT 2 cut(s) 1286, 1692
SspMI CTAG 7 cut(s) 633, 737, 909, 1641, 1647, 2376, 2697
SstI GAGCTC 2 cut(s) 189, 1165
StyD4I CCNGG 8 cut(s) 721, 854, 1057, 1106, 1227, 1350, 1437, 2836
StyI CCWWGG 5 cut(s) 522, 666, 1210, 1513, 2609
TaaI ACNGT 3 cut(s) 1303, 1552, 1978
TaiI ACGT 8 cut(s) 78, 131, 161, 1188, 1459, 1617, 1984, 2671
TaqI TCGA 5 cut(s) 31, 163, 168, 270, 291
TaqII GACCGA 1 cut(s) 1049
TatI WGTACW 4 cut(s) 216, 377, 1279, 2699
TauI GCSGC 3 cut(s) 351, 2094, 2248
TscAI CASTG 2 cut(s) 1306, 1513
TseFI GTSAC 4 cut(s) 1508, 2060, 2692, 2780
TseI GCWGC 4 cut(s) 240, 905, 1069, 1580
Tsp45I GTSAC 4 cut(s) 1508, 2060, 2692, 2780
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 2 cut(s) 1306, 1513
Van91I CCANNNNNTGG 1 cut(s) 1234
VpaK11BI GGWCC 5 cut(s) 851, 943, 1032, 1225, 1757
VspI ATTAAT 1 cut(s) 917
XagI CCTNNNNNAGG 4 cut(s) 492, 1848, 2168, 2369
XbaI TCTAGA 1 cut(s) 736
XceI RCATGY 2 cut(s) 2061, 2488
XcmI CCANNNNNNNNNTGG 1 cut(s) 2015
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 2 cut(s) 168, 2856
XmnI GAANNNNTTC 2 cut(s) 2184, 2969
XspI CTAG 7 cut(s) 633, 737, 909, 1641, 1647, 2376, 2697
ZrmI AGTACT 2 cut(s) 218, 2701
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.