pycom10g29010

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006300
Physical Location & Seq
Forward (+)
29451057 .. 29455921
4865 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g29010.1

Sequence Viewer

Length: 3057 bp
ATGAAGCTGGTGGTTCAAGTATTGGGGGCTCGGGATCTACCAGCGATGGATCTCAACGGCTTTATCGATCCGTACGTGAAGGTGCAGCTGGGGAAGCACAAGCTCAGGACCAAAGTGGTCAAGAAGACCTTAAACCCCTATTGGGGGGAAGAGTTCACCTTCAGGGTCGACGACCTCAACGACGAGCTCGTCATCTCTGTCCTCGACGAGGACAAGTACTTCAACGACGACTTCGTTGGCTACGTCCGCATCCCTGTTTCCCAAGTCTTCGATGCCCGCAACAAGTCCCTTGAAACTGCTTGGCACCCTCTCCACCCCAAGAGTAAAAAATCCAAGAACAAGGATTGCGGCGAGATTCTTCTTGCAATACATTTTTCTTCAAACAATTCATTCGTGGACTCGGCTTCTGAGGGTGGTGACATAGGAAGTGAATCACCTTCAAGGTCTTTCAGTGGTGTTTCAGAATCAGCTTCTCCTGTGAGAGTGAGACCAGAAGAAACAGCATCTTTTAAGGACTTTAAGGAAGAAAAGATTTGTTCTCAGAAGACCTTTGCTGGTCGCATAGCTCAGATATTCAATAAAAATCCAGATTTGCTTTCAGCCTCCTCTAGCAGAGTGGACTTGTCCGAGCTGTCTGAAACTGCTAAATCCGAAGTTTGTGAGAGCAGCCCTGAGGATCAGTCCTCTTCCGCTACATTTGAAGAATTGATGAAAACAATACAGTCACGAGATCAAGAGAGTGAAACCCCAACCAACTTACCAGGAGGAGTGCTTGTGGATCAACTGTACGTCACTCCACCCCAAGACCTAAACACATTACTATTTTCAACAGATTCAGGTTTTCAAAAAGAGGTGGCAGATGCGCAGGGAACTACAGAATTGGATCCGGGGCAATGGAAATTAGATACCAGCACCGAGAGCGTCAAAAGAGTGGTGACATACGTTAAGGCTGCAACTAAGTTGATAAAGGCTGTCAAAGGAACTGAGGACCAAACGTATCTCAAAGCCGACGGGAAGGTTTTTGCAGTTTTAGCAAGCGTAAGCACTCCTGATGTTCCCTATGGGCGAACTTTTAAGACGGAGTTGCTCTACTGCATAACACCTGGCCCCGAGCTGCCATCAGGAGAGCAATCTTCACGTCTGGTAATATCCTGGCGGATGAATTTTCTGCAGAGCACCATGATGAAAGGAATGATAGAAAATGGAGCTCGACAAGGTTTAAAGGAAAGTTTTGACCACTATGCTACTATATTGTCTCAGAATGTTAAGCCAGTCGATTCAAAGGATCTTGGATCCAATAAGGATCAGGTTTTGGCATCACTGCAAGCTGAACCCCAGTCTGATTGGAAGCTGGCAGTCCAGTATTTTGCCAATTTCACTGTGATCTCCACATTATTTATTGGCTTGTATATGTTTGTACACATCTGGCTTGCCCAACCCAGCACTATTCAAGGGCTGGAATTTGTTGGGCTTGACTTGCCGGATTCAATTGGTGAATTCATCGTCTGTGGTGTCCTGGTTCTTCAAGGAGAAAGGGTGCTGGGGTTGATCTCACGCTTCATGCAGGCCAGAGCACAAAACGGCAGTGATCATGGAGTCAAAGCCCAAGGAGATGGTTGGTTGCTAACTGTTGCATTGATTGAAGGAAGTAACATAGCAGCTGTTGATTCGAGTGGGTTTTCTGATCCATACGTGGTGTTCACTTGCAATGGGAAAACTAGAACCAGCTCAATCAAGTTCCAGAAATGCGATCCTACATGGAATGAAATATTTGAGTTTGATGCAATGGATGAACCTCCTTCTGTGTTGGATGTGGAAGTTTATGATTTTGATGGACCTTTTGATGAAGCTATCTCTCTGGGCCATGCTGAAATCAATTTTGTTAAAACTAATATATCAGATTTAGCTGACTTGTGGGTTCCTCTTCAAGGAAAGTTAGCTCAGGCATGCCAGTCAAAACTCCACCTGAGAATTTTCTTAAACAATACAAGAGGTGGCAACGTCGTAAATCAATTTTTAACTAAGATGGAGAAGGAGGTGGGAAAGAAGATAACTGTGCGGTCTCCTCAAACAAATTCAGCATTCCAAAAACTGTTTGGGCTTCCACCGGAGGAATTTCTCATCAACGACTTTACCTGTCACTTGAAACGAAAAATGCCCCTGCAGGGCCGCCTATTTCTTTCTGCAAGAATAATTGGTTTCCATGCAAATTTATTTGGTCACAAGACAAAGTTCTTCTTCCTGTGGGAGGATATAGAGGATATTCAAGTTGTACCTCCAACTCTGTCATCTATGGGCAGTCCAATTATAGTCATGACTCTGCGGCAGGGTAGAGGTATGGATGCAAGACATGGTGCAAAGACACAAGATGAGGAAGGCAGGCTCAAGTTCCATTTCCAGTCCTTTGTATCTTTCAATGTAGCTAACAGGACAATCATGGCTCTTTGGAAGGCTAGGTCTTTGAGTCCTGAGCAGAAGGTAAAAATAGTTGAAGAAGAATCCGATGTCAAGATCCAAGCTGAAGAGAGTGGGTCTTTCTTGGGCCTTGATGATGTCAGCACGTCTGAGGTTTATTCTGCAGCTCATTCCGTTCCTACAAATTTCTTCATAGAGCTATTTGGCGGAGGTGAATTGGACCGTAGAGTTATGGAGAAAGCTGGTTGTCTTAACTACTCTTATACCCCATGGGAATCAGAGAAGGGCGATGTCTGTGTGAGGCAAATATATTACAGATTTGATAAACGTGTGTCCCAATATAGAGGAGAGGTGACTAGTACTCAGCAAAAATCCCGACTCCCTGATAGAAATGGTTGGCTTGTACAAGAGGTCTCGACCCTCCATGCAGTACCGCTTGGCGACTATTTCAATCTTCACGTTCGATACCAAATCGAGGATTTGCCCTCAACACCAAAGGGATGCCAGGTAAAAGTATACTTTGGGGTTGAGTGGCTCAAAAGCACTCGGCATCAGAAAAGAATTACAAAAAACGTGCTTAAGAATTTGCACGATCGCCTGAAGGACACCTTCAGTGTAGTTGAGAAGGAATTCACTATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1019

Amino Acids

114.08

Weight (kDa)

5.72

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 103 2.3e-30 C2 domain
VASt PF16016 258 - 406 1.3e-33 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 541 - 642 1.8e-16 C2 domain
GRAM PF02893 694 - 802 1.8e-19 GRAM domain
VASt PF16016 864 - 999 2.5e-23 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 188
Acc16I TGCGCA 1 cut(s) 864
AccB1I GGYRCC 1 cut(s) 303
AccI GTMKAC 2 cut(s) 168, 2931
AcuI CTGAAG 4 cut(s) 145, 2541, 3010, 3035
AfaI GTAC 8 cut(s) 74, 218, 788, 1419, 2274, 2776, 2820, 2847
AfiI CCNNNNNNNGG 8 cut(s) 142, 143, 144, 208, 1928, 2165, 2248, 2890
AflII CTTAAG 1 cut(s) 2993
AflIII ACRYGT 1 cut(s) 2743
AhlI ACTAGT 1 cut(s) 2771
AjiI CACGTC 2 cut(s) 1139, 2562
AjnI CCWGG 5 cut(s) 760, 1102, 1151, 1515, 2919
Alw21I GWGCWC 4 cut(s) 189, 1178, 1210, 1576
Alw26I GTCTC 4 cut(s) 481, 1260, 2067, 2833
Ama87I CYCGRG 2 cut(s) 30, 1109
AoxI GGCC 5 cut(s) 1105, 1566, 1861, 2167, 2542
ApeKI GCWGC 6 cut(s) 85, 666, 950, 1114, 1658, 2579
Asp700I GAANNNNTTC 1 cut(s) 3044
AspLEI GCGC 1 cut(s) 865
AspS9I GGNCC 8 cut(s) 108, 988, 1106, 1835, 1861, 2167, 2542, 2635
AsuC2I CCSGG 1 cut(s) 888
AsuHPI GGTGA 7 cut(s) 148, 426, 428, 946, 1505, 2639, 2779
AvaI CYCGRG 2 cut(s) 30, 1109
AvaII GGWCC 4 cut(s) 108, 988, 1835, 2635
AxyI CCTNAGG 1 cut(s) 672
BamHI GGATCC 2 cut(s) 883, 1292
BanI GGYRCC 1 cut(s) 303
BanII GRGCYC 3 cut(s) 31, 189, 1210
BauI CACGAG 1 cut(s) 726
BbsI GAAGAC 3 cut(s) 131, 259, 551
Bbv12I GWGCWC 4 cut(s) 189, 1178, 1210, 1576
BbvI GCAGC 6 cut(s) 97, 678, 937, 1101, 1670, 2591
BccI CCATC 5 cut(s) 40, 1126, 1607, 1826, 2020
BceAI ACGGC 2 cut(s) 73, 1597
BciT130I CCWGG 5 cut(s) 762, 1104, 1153, 1517, 2921
BclI TGATCA 1 cut(s) 1588
BcnI CCSGG 1 cut(s) 888
BcoDI GTCTC 4 cut(s) 481, 1260, 2067, 2833
BcuI ACTAGT 1 cut(s) 2771
BfaI CTAG 4 cut(s) 609, 1719, 2454, 2772
BfmI CTRYAG 4 cut(s) 873, 1169, 2162, 2577
BfrI CTTAAG 1 cut(s) 2993
BisI GCNGC 9 cut(s) 86, 349, 667, 951, 1115, 1659, 2170, 2324, 2580
BlsI GCNGC 9 cut(s) 87, 350, 668, 952, 1116, 1660, 2171, 2325, 2581
BmcAI AGTACT 2 cut(s) 218, 2776
Bme1390I CCNGG 6 cut(s) 762, 888, 1104, 1153, 1517, 2921
Bme18I GGWCC 4 cut(s) 108, 988, 1835, 2635
BmeT110I CYCGRG 2 cut(s) 30, 1109
BmgBI CACGTC 2 cut(s) 1139, 2562
BmgT120I GGNCC 8 cut(s) 108, 988, 1106, 1835, 1861, 2167, 2542, 2635
BmiI GGNNCC 5 cut(s) 305, 885, 1108, 1294, 1920
BmrFI CCNGG 6 cut(s) 762, 888, 1104, 1153, 1517, 2921
BmrI ACTGGG 1 cut(s) 1330
BmsI GCATC 9 cut(s) 258, 262, 512, 850, 1325, 1771, 2332, 2906, 2974
BmuI ACTGGG 1 cut(s) 1330
BpiI GAAGAC 3 cut(s) 131, 259, 551
Bpu10I CCTNAGC 3 cut(s) 104, 1941, 2469
BpuEI CTTGAG 1 cut(s) 2369
BpuMI CCSGG 1 cut(s) 888
Bsa29I ATCGAT 1 cut(s) 66
BsaAI YACGTR 2 cut(s) 76, 1693
BsaI GGTCTC 3 cut(s) 481, 2067, 2833
BsaJI CCNNGG 3 cut(s) 887, 1606, 2684
BsaWI WCCGGW 1 cut(s) 2107
BsaXI ACNNNNNCTCC 4 cut(s) 759, 789, 1521, 1551
Bsc4I CCNNNNNNNGG 8 cut(s) 142, 143, 144, 208, 1928, 2165, 2248, 2890
Bse1I ACTGG 5 cut(s) 1271, 1336, 1360, 1951, 2398
Bse21I CCTNAGG 1 cut(s) 672
Bse3DI GCAATG 3 cut(s) 899, 1714, 1791
BseBI CCWGG 5 cut(s) 762, 1104, 1153, 1517, 2921
BseCI ATCGAT 1 cut(s) 66
BseDI CCNNGG 3 cut(s) 887, 1606, 2684
BseGI GGATG 6 cut(s) 249, 1164, 1795, 1816, 2347, 2921
BseLI CCNNNNNNNGG 8 cut(s) 142, 143, 144, 208, 1928, 2165, 2248, 2890
BseMI GCAATG 3 cut(s) 899, 1714, 1791
BseNI ACTGG 5 cut(s) 1271, 1336, 1360, 1951, 2398
BseRI GAGGAG 4 cut(s) 595, 780, 2055, 2775
BseXI GCAGC 6 cut(s) 97, 678, 937, 1101, 1670, 2591
BseYI CCCAGC 3 cut(s) 88, 1439, 1540
BsgI GTGCAG 1 cut(s) 104
Bsh1285I CGRYCG 1 cut(s) 3010
BshFI GGCC 5 cut(s) 1107, 1568, 1863, 2169, 2544
BshNI GGYRCC 1 cut(s) 303
BshVI ATCGAT 1 cut(s) 66
BsiEI CGRYCG 1 cut(s) 3010
BsiHKAI GWGCWC 4 cut(s) 189, 1178, 1210, 1576
BsiHKCI CYCGRG 2 cut(s) 30, 1109
BsiSI CCGG 3 cut(s) 887, 1481, 2108
BsiWI CGTACG 1 cut(s) 72
BslFI GGGAC 2 cut(s) 271, 2734
BslI CCNNNNNNNGG 8 cut(s) 142, 143, 144, 208, 1928, 2165, 2248, 2890
BsmAI GTCTC 4 cut(s) 481, 1260, 2067, 2833
BsmFI GGGAC 2 cut(s) 271, 2734
BsmI GAATGC 1 cut(s) 2081
BsnI GGCC 5 cut(s) 1107, 1568, 1863, 2169, 2544
Bso31I GGTCTC 3 cut(s) 481, 2067, 2833
BsoBI CYCGRG 2 cut(s) 30, 1109
Bsp1286I GDGCHC 5 cut(s) 31, 189, 1178, 1210, 1576
Bsp1407I TGTACA 2 cut(s) 1417, 2818
Bsp19I CCATGG 1 cut(s) 2684
BspANI GGCC 5 cut(s) 1107, 1568, 1863, 2169, 2544
BspDI ATCGAT 1 cut(s) 66
BspHI TCATGA 1 cut(s) 2313
BspLI GGNNCC 5 cut(s) 305, 885, 1108, 1294, 1920
BspMAI CTGCAG 3 cut(s) 1173, 2166, 2581
BspT107I GGYRCC 1 cut(s) 303
BspTI CTTAAG 1 cut(s) 2993
BspTNI GGTCTC 3 cut(s) 481, 2067, 2833
BsrDI GCAATG 3 cut(s) 899, 1714, 1791
BsrGI TGTACA 2 cut(s) 1417, 2818
BsrI ACTGG 5 cut(s) 1271, 1336, 1360, 1951, 2398
BssECI CCNNGG 3 cut(s) 887, 1606, 2684
BssNAI GTATAC 1 cut(s) 2932
BssSI CACGAG 1 cut(s) 726
BssT1I CCWWGG 2 cut(s) 1606, 2684
Bst1107I GTATAC 1 cut(s) 2932
Bst2BI CACGAG 1 cut(s) 726
Bst2UI CCWGG 5 cut(s) 762, 1104, 1153, 1517, 2921
Bst4CI ACNGT 7 cut(s) 723, 786, 1381, 1630, 2056, 2094, 2639
Bst6I CTCTTC 4 cut(s) 144, 691, 1929, 2517
BstAFI CTTAAG 1 cut(s) 2993
BstAUI TGTACA 2 cut(s) 1417, 2818
BstBAI YACGTR 2 cut(s) 76, 1693
BstC8I GCNNGC 8 cut(s) 277, 1036, 1326, 1353, 1431, 1566, 1948, 2381
BstDSI CCRYGG 1 cut(s) 2684
BstENI CCTNNNNNAGG 3 cut(s) 206, 1926, 2246
BstF5I GGATG 6 cut(s) 249, 1164, 1795, 1816, 2347, 2921
BstHHI GCGC 1 cut(s) 865
BstMAI GTCTC 4 cut(s) 481, 1260, 2067, 2833
BstMCI CGRYCG 1 cut(s) 3010
BstMWI GCNNNNNNNGC 5 cut(s) 94, 246, 918, 1031, 1477
BstNI CCWGG 5 cut(s) 762, 1104, 1153, 1517, 2921
BstNSI RCATGY 1 cut(s) 1950
BstSCI CCNGG 6 cut(s) 760, 886, 1102, 1151, 1515, 2919
BstSFI CTRYAG 4 cut(s) 873, 1169, 2162, 2577
BstV1I GCAGC 6 cut(s) 97, 678, 937, 1101, 1670, 2591
BstV2I GAAGAC 3 cut(s) 131, 259, 551
BstX2I RGATCY 6 cut(s) 34, 49, 883, 1285, 1292, 2511
BstXI CCANNNNNNTGG 1 cut(s) 1613
BstYI RGATCY 6 cut(s) 34, 49, 883, 1285, 1292, 2511
BstZ17I GTATAC 1 cut(s) 2932
Bsu15I ATCGAT 1 cut(s) 66
Bsu36I CCTNAGG 1 cut(s) 672
BsuRI GGCC 5 cut(s) 1107, 1568, 1863, 2169, 2544
BsuTUI ATCGAT 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 2684
BtgZI GCGATG 2 cut(s) 59, 2718
BtrI CACGTC 2 cut(s) 1139, 2562
BtsCI GGATG 6 cut(s) 249, 1164, 1795, 1816, 2347, 2921
BtsI GCAGTG 2 cut(s) 1319, 1591
BtsIMutI CAGTG 5 cut(s) 457, 1319, 1377, 1591, 3034
Cac8I GCNNGC 8 cut(s) 277, 1036, 1326, 1353, 1431, 1566, 1948, 2381
CciI TCATGA 1 cut(s) 2313
CfoI GCGC 1 cut(s) 865
Cfr13I GGNCC 8 cut(s) 108, 988, 1106, 1835, 1861, 2167, 2542, 2635
ClaI ATCGAT 1 cut(s) 66
CseI GACGC 1 cut(s) 910
Csp6I GTAC 8 cut(s) 73, 217, 787, 1418, 2273, 2775, 2819, 2846
CviQI GTAC 8 cut(s) 73, 217, 787, 1418, 2273, 2775, 2819, 2846
DraI TTTAAA 1 cut(s) 1221
DrdI GACNNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 1 cut(s) 188
Eam1104I CTCTTC 4 cut(s) 144, 691, 1929, 2517
EarI CTCTTC 4 cut(s) 144, 691, 1929, 2517
EciI GGCGGA 2 cut(s) 1171, 2637
Ecl136II GAGCTC 2 cut(s) 187, 1208
Eco130I CCWWGG 2 cut(s) 1606, 2684
Eco24I GRGCYC 3 cut(s) 31, 189, 1210
Eco31I GGTCTC 3 cut(s) 481, 2067, 2833
Eco47I GGWCC 4 cut(s) 108, 988, 1835, 2635
Eco53kI GAGCTC 2 cut(s) 187, 1208
Eco57I CTGAAG 4 cut(s) 145, 2541, 3010, 3035
Eco81I CCTNAGG 1 cut(s) 672
Eco88I CYCGRG 2 cut(s) 30, 1109
EcoICRI GAGCTC 2 cut(s) 187, 1208
EcoNI CCTNNNNNAGG 3 cut(s) 206, 1926, 2246
EcoRI GAATTC 2 cut(s) 1496, 3044
EcoRII CCWGG 5 cut(s) 760, 1102, 1151, 1515, 2919
EcoT14I CCWWGG 2 cut(s) 1606, 2684
EcoT38I GRGCYC 3 cut(s) 31, 189, 1210
ErhI CCWWGG 2 cut(s) 1606, 2684
FalI AAGNNNNNCTT 6 cut(s) 113, 145, 2222, 2254, 3008, 3040
FaqI GGGAC 2 cut(s) 271, 2734
FauI CCCGC 1 cut(s) 284
FbaI TGATCA 1 cut(s) 1588
FblI GTMKAC 2 cut(s) 168, 2931
Fnu4HI GCNGC 9 cut(s) 86, 349, 667, 951, 1115, 1659, 2170, 2324, 2580
FokI GGATG 6 cut(s) 236, 1171, 1802, 1823, 2354, 2928
FriOI GRGCYC 3 cut(s) 31, 189, 1210
Fsp4HI GCNGC 9 cut(s) 86, 349, 667, 951, 1115, 1659, 2170, 2324, 2580
FspBI CTAG 4 cut(s) 609, 1719, 2454, 2772
FspI TGCGCA 1 cut(s) 864
GlaI GCGC 1 cut(s) 864
GluI GCNGC 9 cut(s) 86, 349, 667, 951, 1115, 1659, 2170, 2324, 2580
GsaI CCCAGC 3 cut(s) 92, 1443, 1544
HaeIII GGCC 5 cut(s) 1107, 1568, 1863, 2169, 2544
HapII CCGG 3 cut(s) 887, 1481, 2108
HgaI GACGC 1 cut(s) 910
HhaI GCGC 1 cut(s) 865
Hin6I GCGC 1 cut(s) 863
HinP1I GCGC 1 cut(s) 863
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HpaII CCGG 3 cut(s) 887, 1481, 2108
HphI GGTGA 7 cut(s) 148, 426, 428, 946, 1505, 2639, 2779
Hpy166II GTNNAC 7 cut(s) 156, 169, 397, 619, 1420, 1701, 2932
Hpy8I GTNNAC 7 cut(s) 156, 169, 397, 619, 1420, 1701, 2932
Hpy99I CGWCG 6 cut(s) 173, 185, 209, 230, 1013, 2006
HpyCH4III ACNGT 7 cut(s) 723, 786, 1381, 1630, 2056, 2094, 2639
HpyF10VI GCNNNNNNNGC 5 cut(s) 94, 246, 918, 1031, 1477
HspAI GCGC 1 cut(s) 863
Ksp22I TGATCA 1 cut(s) 1588
LmnI GCTCC 1 cut(s) 1205
Lsp1109I GCAGC 6 cut(s) 97, 678, 937, 1101, 1670, 2591
LweI GCATC 9 cut(s) 258, 262, 512, 850, 1325, 1771, 2332, 2906, 2974
MaeI CTAG 4 cut(s) 609, 1719, 2454, 2772
MaeIII GTNAC 8 cut(s) 416, 723, 790, 934, 1649, 2138, 2219, 2767
MfeI CAATTG 1 cut(s) 1488
MflI RGATCY 6 cut(s) 34, 49, 883, 1285, 1292, 2511
MhlI GDGCHC 5 cut(s) 31, 189, 1178, 1210, 1576
MlyI GAGTC 5 cut(s) 392, 1605, 2311, 2473, 2787
MmeI TCCRAC 2 cut(s) 1788, 2303
MroXI GAANNNNTTC 1 cut(s) 3044
MslI CAYNNNNRTG 1 cut(s) 1181
MspA1I CMGCKG 2 cut(s) 88, 1661
MspCI CTTAAG 1 cut(s) 2993
MspI CCGG 3 cut(s) 887, 1481, 2108
MspR9I CCNGG 6 cut(s) 762, 888, 1104, 1153, 1517, 2921
MunI CAATTG 1 cut(s) 1488
Mva1269I GAATGC 1 cut(s) 2081
MvaI CCWGG 5 cut(s) 762, 1104, 1153, 1517, 2921
MwoI GCNNNNNNNGC 5 cut(s) 94, 246, 918, 1031, 1477
NciI CCSGG 1 cut(s) 888
NcoI CCATGG 1 cut(s) 2684
NlaIV GGNNCC 5 cut(s) 305, 885, 1108, 1294, 1920
NmeAIII GCCGAG 2 cut(s) 380, 2941
NmuCI GTSAC 7 cut(s) 416, 723, 790, 934, 2138, 2219, 2767
NsbI TGCGCA 1 cut(s) 864
NspI RCATGY 1 cut(s) 1950
PaeI GCATGC 1 cut(s) 1950
PagI TCATGA 1 cut(s) 2313
PcsI WCGNNNNNNNCGW 6 cut(s) 63, 72, 177, 186, 231, 240
PctI GAATGC 1 cut(s) 2081
PdmI GAANNNNTTC 1 cut(s) 3044
PfeI GAWTC 9 cut(s) 355, 431, 464, 833, 1277, 1484, 1667, 2498, 2690
Pfl23II CGTACG 1 cut(s) 72
PkrI GCNGC 9 cut(s) 87, 350, 668, 952, 1116, 1660, 2171, 2325, 2581
Ple19I CGATCG 1 cut(s) 3010
PleI GAGTC 5 cut(s) 392, 1604, 2311, 2472, 2787
PpsI GAGTC 5 cut(s) 392, 1604, 2311, 2472, 2787
Ppu21I YACGTR 2 cut(s) 76, 1693
Psp124BI GAGCTC 2 cut(s) 189, 1210
Psp6I CCWGG 5 cut(s) 760, 1102, 1151, 1515, 2919
PspFI CCCAGC 3 cut(s) 88, 1439, 1540
PspGI CCWGG 5 cut(s) 760, 1102, 1151, 1515, 2919
PspLI CGTACG 1 cut(s) 72
PspN4I GGNNCC 5 cut(s) 305, 885, 1108, 1294, 1920
PspPI GGNCC 8 cut(s) 108, 988, 1106, 1835, 1861, 2167, 2542, 2635
PstI CTGCAG 3 cut(s) 1173, 2166, 2581
PsuI RGATCY 6 cut(s) 34, 49, 883, 1285, 1292, 2511
PvuI CGATCG 1 cut(s) 3010
PvuII CAGCTG 2 cut(s) 88, 1661
RsaI GTAC 8 cut(s) 74, 218, 788, 1419, 2274, 2776, 2820, 2847
RsaNI GTAC 8 cut(s) 73, 217, 787, 1418, 2273, 2775, 2819, 2846
RseI CAYNNNNRTG 1 cut(s) 1181
SacI GAGCTC 2 cut(s) 189, 1210
SalI GTCGAC 1 cut(s) 167
SatI GCNGC 9 cut(s) 86, 349, 667, 951, 1115, 1659, 2170, 2324, 2580
Sau96I GGNCC 8 cut(s) 108, 988, 1106, 1835, 1861, 2167, 2542, 2635
SbfI CCTGCAGG 1 cut(s) 2166
ScaI AGTACT 2 cut(s) 218, 2776
SchI GAGTC 5 cut(s) 392, 1605, 2311, 2473, 2787
ScrFI CCNGG 6 cut(s) 762, 888, 1104, 1153, 1517, 2921
SdaI CCTGCAGG 1 cut(s) 2166
SduI GDGCHC 5 cut(s) 31, 189, 1178, 1210, 1576
SfaNI GCATC 9 cut(s) 258, 262, 512, 850, 1325, 1771, 2332, 2906, 2974
SfcI CTRYAG 4 cut(s) 873, 1169, 2162, 2577
SinI GGWCC 4 cut(s) 108, 988, 1835, 2635
SmiMI CAYNNNNRTG 1 cut(s) 1181
SmlI CTYRAG 2 cut(s) 2384, 2993
SmoI CTYRAG 2 cut(s) 2384, 2993
SpeI ACTAGT 1 cut(s) 2771
SphI GCATGC 1 cut(s) 1950
Sse8387I CCTGCAGG 1 cut(s) 2166
SspI AATATT 1 cut(s) 1770
SspMI CTAG 4 cut(s) 609, 1719, 2454, 2772
SstI GAGCTC 2 cut(s) 189, 1210
StyD4I CCNGG 6 cut(s) 760, 886, 1102, 1151, 1515, 2919
StyI CCWWGG 2 cut(s) 1606, 2684
TaaI ACNGT 7 cut(s) 723, 786, 1381, 1630, 2056, 2094, 2639
TatI WGTACW 4 cut(s) 216, 1417, 2774, 2818
TauI GCSGC 3 cut(s) 351, 2172, 2326
TfiI GAWTC 9 cut(s) 355, 431, 464, 833, 1277, 1484, 1667, 2498, 2690
TscAI CASTG 5 cut(s) 457, 1326, 1384, 1591, 3034
TseFI GTSAC 7 cut(s) 416, 723, 790, 934, 2138, 2219, 2767
TseI GCWGC 6 cut(s) 85, 666, 950, 1114, 1658, 2579
Tsp45I GTSAC 7 cut(s) 416, 723, 790, 934, 2138, 2219, 2767
TspGWI ACGGA 3 cut(s) 60, 1094, 2578
TspRI CASTG 5 cut(s) 457, 1326, 1384, 1591, 3034
Vha464I CTTAAG 1 cut(s) 2993
VpaK11BI GGWCC 4 cut(s) 108, 988, 1835, 2635
XagI CCTNNNNNAGG 3 cut(s) 206, 1926, 2246
XceI RCATGY 1 cut(s) 1950
XcmI CCANNNNNNNNNTGG 1 cut(s) 2093
XmiI GTMKAC 2 cut(s) 168, 2931
XmnI GAANNNNTTC 1 cut(s) 3044
XspI CTAG 4 cut(s) 609, 1719, 2454, 2772
ZrmI AGTACT 2 cut(s) 218, 2776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.