Rh5DG002600

C2 and GRAM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006300
Physical Location & Seq
Reverse (-)
211576 .. 216342
4767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG002600.1

Sequence Viewer

Length: 3072 bp
ATGAAGCTGGTGGTTCAAGTGATGGGGGCTCGAGATCTGCCGGCAATGGATCTAAACGGGCTGAGTGATCCGTACGTGAAGGTGAAGATGGGAAAGCAGAAGTTCAAGACCAAAGTGGTGAAGAAGACCTTAAATCCATATTGGGGTGAAGAGTTCACGTTTCGAGTCGACGACCTCAACGACGAGCTCCTCATCTCTTGTTTGGATGAAGATAAGTACTTCAACGACGACTTCGTGGGCTGCGTCAAATTCCCTGTTTCCCAAGTCTTCGATTCCCCCAACAAGTGCCTCGACACTTCTTGGCACCCTCTCCAGCCCAAGAGCAAGAAGCCCAAGCACAAGGATTGCGGCGAGATTCTTCTTAATATCTCTCTCAGTACAAACAATGCATTCTCCGACTCCGCTTCCGAGGGTGATCATATAAGGAGGGATTCCGACATTGGAGGTGAATCACCCTCAAGGTTATCCGAAACGGCTTCCCCTCAGAGAGGCAAGCTTGATGAAGTTCCATTCTCCAAGGAAGATAAAGAAAAGGAAAAGACTCTTGCCCAGAAATCCCTTGCTGGCCGACTTGTTCAGATGTTCAATAAAAATCCTGACGCACCTCCAATATCTTCAAACCACTCTAGCAAAGCTGACTTGACTGAGCTTGTTGAAACCAAGGCCGACATTTCTGAGGCCACTTCTGAGGATCACTCCTCTTGTGTTCCTTTTGAAGAGCTAATGAAAACAATGCAGTTACGCGATCAGGCTACTGAAACCCCTGAGAACCTACCGGGTGGAGTGCTTCTAGATCAAATGTATGTTACTGAACCCAAACACCTCAACACTTTACTCTTTTCACCGGATTCTACTTTTCCAAAGGCGTTGGCAGATCTGCATGGAACTACAGAATTGGAACAAGGACCGTGGAAATTTGAAAACGACAGCTTAAAAAGAGTGGTTACATATGTTAAGGCAGCTGGCAAATTAATCAAGGCTTGCAAAGGAAGTGAGGACCAAACATATCTCAAAGCCGATGGGAAAGTTTTTGCAGTTCTATCAAGTGTAAGCACTCCTGATGTTCCATATGGGAAAACTTTTAGGACGGAGTTGCTCTACTGCATTACACCTGGGCCTGAGCTGCCATCGGGAGAACAATCTACACGCTTTGTAATATCCTGGCGCATGAATTTTCTGCAGAGCACCATGATGAAAGGAATGATAGAAAATGGAGCTCGGCAAGGTTTAAAGGACAGTTATGAGCAGTATGCAACTTTATTATCGCAGAACGTTAAGCCAGCTGATTCGAAAGACCTTGGATCCAATAAGGACCAGGTTTTGGCATCATTGCAGGCTGAATCCCAGTCTGATTGGAAGCTGGCAGTACAATATTTTGCTAATTTCACTGTGGTCTCCACATTTTTTATTGGATTGTATATGCTGGTACACATCTACCTGGCTACACCCAGCACTATTCAAGGGTTGGAGTTTGTTGGGCTTGACTTGCCAGATTCAATTGGTGAATTCATTGTTTGTGGTGTCCTGGTACTTCAAGGAGAACGTGTGCTGGGTTTGATCTCTCGCTTTATGCAGGCCAGAGTGCAGAAAGGCAGTGACCATGGAGTCAAAGCCCGAGGAGATGGTTGGTTGCTAACTGTTGCCTTGATTGAAGGAAGTAATATAGCAGCTGTTGATTCAAGTGGGTTCTCTGATCCATACGTGGTGTTTTCTTGCAATGGAAAAGCTAGAACTAGCTCAATCAAGTTCCAGAAATGCGACCCTATGTGGAATGAAATATTTGAGTTTGATGCAATGGATGAGCCTCCTTCTGTGCTGGATGTGGAAATTTATGATTTTGATGGTCCTTTTGATGAAGCTACCTCTCTGGGCCATGCTGAAATCAATTTTGTGAAAACCAATATATCAGATTTAGCTGATATGTGGATTCCTCTTCAAGGAAAGTTAGCCCAGGCTTGTCAGTCAAAACTCCACTTGAGAATTTTCTTAAACAATACAAGAGGTGGCAATGTTGTTAATCACTTTTTAAATAAGATGGAGAAGGAGGTTGGAAAGAAGATTACTGTACGTTCTCCTCAAACAAATTCAGCATTCCAAAAACTCTTTGGGCTTCCACCAGAGGAATTTCTTATCAACGACTTTACATGTCACTTGAAACGAAAAATGCCCCTGCAGGGCCGCCTATTTCTTTCTGCGAGAATAATTGGGTTCCATGCAAATTTATTTGGTCGCAAGACAAAGTTCTTTTTCCTCTGGGAGGATATAGAAGATATTCATATTGTTCCTCCAACTCTGTCATCTATGGGTAGTCCAACTATAGTCATGACTCTGCGGCAAGGTAGAGGTATGGATGCAAGGCATGGTGCAAAGACACAGGATGAGGAAGGCAGGCTGAAGTTCCACTTCCAGTCCTTCGTATCCTTCAATGTAGCGAACAGGACAATTATGGCCCTGTGGAAGGCTAGATCTTTGAGTCCTGAGCAGAAGGTGCAAATAATAGAAGAAGAATCAGAAGTCAAGAGCCTCCAAACTGAAGAGAGTGGGTCTTTCTTGGGCCTTGATGATGTCAGCATGTCTGAGGTTTATTCATCCTTGCATTCAGTTCCTACAAATTTCTTCGCAGAGCTATTTGGTGGAGGTGAATTGGATCGTAGAGTTATGGAGAAAGCTGGTTGTCTTAACTATTCTCACACCCCATGGGAATCAGAGAAGGGCGATGTCTATGTGAGGCAAATATATTACAGATATGACAAACGTGTTTCCCAATATAGAGGAGAGGTGACTAGTACTCAGCAAAAATCCCGCCTTTCTGATAGAAATGGTTGGCTTTTTCAAGAAGTCATGACCCTCCATGCAATTCCACTTGGTGACTATTTCAATGTTCACATCCGATACCAAATTGAGGACTCATCCTCAACACCACCAGGGTGTCTGGTAAAAGTATACTTTGGCATTGAGTGGCTCAAAAGCACTAAGCATCAGAAAAGAATTACAAAAAATGTTCTCAAGAATCTGCAAGATCGCCTAAAGGTCTCCTTTGCTGTAGTTGAGAAGGAGTTCACATCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 DUF4782 GRAM

Protein Analysis

1023

Amino Acids

115.1

Weight (kDa)

6.07

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 2 - 93 1.9e-29 C2 domain
VASt PF16016 263 - 409 9.9e-32 VAD1 Analog of StAR-related lipid transfer domain
C2 PF00168 544 - 645 4e-16 C2 domain
GRAM PF02893 697 - 804 3.2e-17 GRAM domain
VASt PF16016 869 - 1003 7.6e-23 VAD1 Analog of StAR-related lipid transfer domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1604
AccB1I GGYRCC 1 cut(s) 303
AccB7I CCANNNNNTGG 1 cut(s) 1321
AccI GTMKAC 2 cut(s) 168, 2943
AccII CGCG 1 cut(s) 744
AciI CCGC 5 cut(s) 348, 402, 2179, 2332, 2803
AclI AACGTT 1 cut(s) 1272
AclWI GGATC 7 cut(s) 57, 62, 699, 1296, 1309, 1688, 2655
AcoI YGGCCR 1 cut(s) 565
AcuI CTGAAG 2 cut(s) 2414, 2553
AdeI CACNNNGTG 1 cut(s) 2867
AfaI GTAC 8 cut(s) 74, 218, 379, 1368, 1428, 1530, 2067, 2788
AfiI CCNNNNNNNGG 8 cut(s) 143, 488, 1321, 1937, 2174, 2257, 2458, 2902
AflIII ACRYGT 3 cut(s) 1543, 2144, 2755
AhlI ACTAGT 1 cut(s) 2783
AjnI CCWGG 7 cut(s) 1111, 1160, 1314, 1437, 1524, 1950, 2923
AleI CACNNNNGTG 1 cut(s) 2926
Alw21I GWGCWC 3 cut(s) 189, 1187, 1219
Alw26I GTCTC 2 cut(s) 1399, 3037
AlwI GGATC 7 cut(s) 57, 62, 699, 1296, 1309, 1688, 2655
Ama87I CYCGRG 2 cut(s) 30, 1614
AoxI GGCC 9 cut(s) 565, 663, 678, 1115, 1575, 1870, 2176, 2448, 2554
ApeKI GCWGC 4 cut(s) 240, 959, 1123, 1667
AseI ATTAAT 1 cut(s) 971
Asp700I GAANNNNTTC 2 cut(s) 2271, 3056
AspLEI GCGC 1 cut(s) 1167
AspS9I GGNCC 9 cut(s) 905, 997, 1115, 1312, 1844, 1870, 2176, 2449, 2554
AsuC2I CCSGG 1 cut(s) 777
AsuII TTCGAA 1 cut(s) 1289
AvaI CYCGRG 2 cut(s) 30, 1614
AvaII GGWCC 4 cut(s) 905, 997, 1312, 1844
BamHI GGATCC 1 cut(s) 1301
BanI GGYRCC 1 cut(s) 303
BanII GRGCYC 3 cut(s) 31, 189, 1219
BbsI GAAGAC 2 cut(s) 131, 259
Bbv12I GWGCWC 3 cut(s) 189, 1187, 1219
BbvI GCAGC 4 cut(s) 227, 971, 1110, 1679
BccI CCATC 7 cut(s) 16, 82, 1013, 1135, 1616, 1835, 2029
BceAI ACGGC 1 cut(s) 489
BciT130I CCWGG 7 cut(s) 1113, 1162, 1316, 1439, 1526, 1952, 2925
BciVI GTATCC 1 cut(s) 2428
BclI TGATCA 1 cut(s) 415
BcnI CCSGG 1 cut(s) 777
BcoDI GTCTC 2 cut(s) 1399, 3037
BcuI ACTAGT 1 cut(s) 2783
BfaI CTAG 6 cut(s) 627, 791, 1728, 1734, 2463, 2784
BfmI CTRYAG 5 cut(s) 888, 1178, 2171, 2316, 3042
BfuI GTATCC 1 cut(s) 2428
BglII AGATCT 3 cut(s) 34, 874, 2465
BisI GCNGC 7 cut(s) 241, 349, 960, 1124, 1668, 2179, 2333
BlsI GCNGC 7 cut(s) 242, 350, 961, 1125, 1669, 2180, 2334
BmcAI AGTACT 2 cut(s) 218, 2788
Bme1390I CCNGG 8 cut(s) 777, 1113, 1162, 1316, 1439, 1526, 1952, 2925
Bme18I GGWCC 4 cut(s) 905, 997, 1312, 1844
BmeT110I CYCGRG 2 cut(s) 30, 1614
BmgT120I GGNCC 9 cut(s) 905, 997, 1115, 1312, 1844, 1870, 2176, 2449, 2554
BmiI GGNNCC 3 cut(s) 305, 1303, 2210
BmrFI CCNGG 8 cut(s) 777, 1113, 1162, 1316, 1439, 1526, 1952, 2925
BmrI ACTGGG 1 cut(s) 1339
BmsI GCATC 4 cut(s) 1334, 1780, 2341, 2986
BmuI ACTGGG 1 cut(s) 1339
BpiI GAAGAC 2 cut(s) 131, 259
BplI GAGNNNNNCTC 2 cut(s) 680, 712
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 2 cut(s) 1119, 2478
Bpu14I TTCGAA 1 cut(s) 1289
BpuEI CTTGAG 3 cut(s) 442, 1996, 2990
BpuMI CCSGG 1 cut(s) 777
BsaAI YACGTR 2 cut(s) 76, 1702
BsaI GGTCTC 2 cut(s) 1399, 3037
BsaWI WCCGGW 1 cut(s) 844
BsaXI ACNNNNNCTCC 2 cut(s) 1530, 1560
Bsc4I CCNNNNNNNGG 8 cut(s) 143, 488, 1321, 1937, 2174, 2257, 2458, 2902
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 1345, 2407
Bse3DI GCAATG 5 cut(s) 51, 1328, 1723, 1800, 2014
BseBI CCWGG 7 cut(s) 1113, 1162, 1316, 1439, 1526, 1952, 2925
BseGI GGATG 8 cut(s) 211, 1804, 1825, 2356, 2383, 2588, 2886, 2909
BseLI CCNNNNNNNGG 8 cut(s) 143, 488, 1321, 1937, 2174, 2257, 2458, 2902
BseMI GCAATG 5 cut(s) 51, 1328, 1723, 1800, 2014
BseNI ACTGG 2 cut(s) 1345, 2407
BseRI GAGGAG 5 cut(s) 179, 688, 1632, 2064, 2787
BseXI GCAGC 4 cut(s) 227, 971, 1110, 1679
BseYI CCCAGC 2 cut(s) 1448, 1549
BsgI GTGCAG 1 cut(s) 1604
Bsh1236I CGCG 1 cut(s) 744
BshFI GGCC 9 cut(s) 567, 665, 680, 1117, 1577, 1872, 2178, 2450, 2556
BshNI GGYRCC 1 cut(s) 303
BsiHKAI GWGCWC 3 cut(s) 189, 1187, 1219
BsiHKCI CYCGRG 2 cut(s) 30, 1614
BsiSI CCGG 3 cut(s) 41, 776, 845
BsiWI CGTACG 1 cut(s) 72
BslI CCNNNNNNNGG 8 cut(s) 143, 488, 1321, 1937, 2174, 2257, 2458, 2902
BsmAI GTCTC 2 cut(s) 1399, 3037
BsmI GAATGC 3 cut(s) 389, 2090, 2596
BsnI GGCC 9 cut(s) 567, 665, 680, 1117, 1577, 1872, 2178, 2450, 2556
Bso31I GGTCTC 2 cut(s) 1399, 3037
BsoBI CYCGRG 2 cut(s) 30, 1614
Bsp119I TTCGAA 1 cut(s) 1289
Bsp1286I GDGCHC 4 cut(s) 31, 189, 1187, 1219
Bsp19I CCATGG 2 cut(s) 1600, 2696
BspACI CCGC 5 cut(s) 348, 402, 2179, 2332, 2803
BspANI GGCC 9 cut(s) 567, 665, 680, 1117, 1577, 1872, 2178, 2450, 2556
BspFNI CGCG 1 cut(s) 744
BspHI TCATGA 2 cut(s) 2322, 2841
BspLI GGNNCC 3 cut(s) 305, 1303, 2210
BspMAI CTGCAG 2 cut(s) 1182, 2175
BspPI GGATC 7 cut(s) 57, 62, 699, 1296, 1309, 1688, 2655
BspQI GCTCTTC 1 cut(s) 711
BspT104I TTCGAA 1 cut(s) 1289
BspT107I GGYRCC 1 cut(s) 303
BspTNI GGTCTC 2 cut(s) 1399, 3037
BsrDI GCAATG 5 cut(s) 51, 1328, 1723, 1800, 2014
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 2 cut(s) 1345, 2407
BssAI RCCGGY 1 cut(s) 40
BssNAI GTATAC 1 cut(s) 2944
BssT1I CCWWGG 5 cut(s) 516, 660, 1297, 1600, 2696
Bst1107I GTATAC 1 cut(s) 2944
Bst2UI CCWGG 7 cut(s) 1113, 1162, 1316, 1439, 1526, 1952, 2925
Bst4CI ACNGT 5 cut(s) 909, 1238, 1390, 1639, 2065
Bst6I CTCTTC 4 cut(s) 144, 711, 1938, 2529
BstAPI GCANNNNNTGC 1 cut(s) 2488
BstBAI YACGTR 2 cut(s) 76, 1702
BstBI TTCGAA 1 cut(s) 1289
BstDSI CCRYGG 3 cut(s) 908, 1600, 2696
BstENI CCTNNNNNAGG 4 cut(s) 486, 1935, 2255, 2456
BstF5I GGATG 8 cut(s) 211, 1804, 1825, 2356, 2383, 2588, 2886, 2909
BstFNI CGCG 1 cut(s) 744
BstHHI GCGC 1 cut(s) 1167
BstMAI GTCTC 2 cut(s) 1399, 3037
BstMWI GCNNNNNNNGC 3 cut(s) 1123, 1486, 2488
BstNI CCWGG 7 cut(s) 1113, 1162, 1316, 1439, 1526, 1952, 2925
BstNSI RCATGY 2 cut(s) 2148, 2575
BstSCI CCNGG 8 cut(s) 775, 1111, 1160, 1314, 1437, 1524, 1950, 2923
BstSFI CTRYAG 5 cut(s) 888, 1178, 2171, 2316, 3042
BstUI CGCG 1 cut(s) 744
BstV1I GCAGC 4 cut(s) 227, 971, 1110, 1679
BstV2I GAAGAC 2 cut(s) 131, 259
BstX2I RGATCY 5 cut(s) 34, 49, 874, 1301, 2465
BstYI RGATCY 5 cut(s) 34, 49, 874, 1301, 2465
BstZ17I GTATAC 1 cut(s) 2944
BsuI GTATCC 1 cut(s) 2428
BsuRI GGCC 9 cut(s) 567, 665, 680, 1117, 1577, 1872, 2178, 2450, 2556
BtgI CCRYGG 3 cut(s) 908, 1600, 2696
BtgZI GCGATG 1 cut(s) 2730
BtsCI GGATG 8 cut(s) 211, 1804, 1825, 2356, 2383, 2588, 2886, 2909
BtsI GCAGTG 1 cut(s) 1600
BtsIMutI CAGTG 2 cut(s) 1386, 1600
CciI TCATGA 2 cut(s) 2322, 2841
CfoI GCGC 1 cut(s) 1167
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 9 cut(s) 905, 997, 1115, 1312, 1844, 1870, 2176, 2449, 2554
CseI GACGC 2 cut(s) 232, 608
CsiI ACCWGGT 1 cut(s) 1314
Csp6I GTAC 8 cut(s) 73, 217, 378, 1367, 1427, 1529, 2066, 2787
CspCI CAANNNNNGTGG 2 cut(s) 890, 925
CviQI GTAC 8 cut(s) 73, 217, 378, 1367, 1427, 1529, 2066, 2787
DraI TTTAAA 2 cut(s) 1230, 2028
DraIII CACNNNGTG 1 cut(s) 2867
DrdI GACNNNNNNGTC 1 cut(s) 1604
DseDI GACNNNNNNGTC 1 cut(s) 1604
EaeI YGGCCR 1 cut(s) 565
Eam1104I CTCTTC 4 cut(s) 144, 711, 1938, 2529
EarI CTCTTC 4 cut(s) 144, 711, 1938, 2529
Ecl136II GAGCTC 2 cut(s) 187, 1217
Eco130I CCWWGG 5 cut(s) 516, 660, 1297, 1600, 2696
Eco24I GRGCYC 3 cut(s) 31, 189, 1219
Eco31I GGTCTC 2 cut(s) 1399, 3037
Eco47I GGWCC 4 cut(s) 905, 997, 1312, 1844
Eco53kI GAGCTC 2 cut(s) 187, 1217
Eco57I CTGAAG 2 cut(s) 2414, 2553
Eco88I CYCGRG 2 cut(s) 30, 1614
EcoICRI GAGCTC 2 cut(s) 187, 1217
EcoNI CCTNNNNNAGG 4 cut(s) 486, 1935, 2255, 2456
EcoRI GAATTC 1 cut(s) 1505
EcoRII CCWGG 7 cut(s) 1111, 1160, 1314, 1437, 1524, 1950, 2923
EcoT14I CCWWGG 5 cut(s) 516, 660, 1297, 1600, 2696
EcoT22I ATGCAT 1 cut(s) 391
EcoT38I GRGCYC 3 cut(s) 31, 189, 1219
ErhI CCWWGG 5 cut(s) 516, 660, 1297, 1600, 2696
FalI AAGNNNNNCTT 6 cut(s) 113, 145, 2387, 2419, 3020, 3052
FauI CCCGC 1 cut(s) 2810
FauNDI CATATG 2 cut(s) 949, 1069
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 2 cut(s) 168, 2943
Fnu4HI GCNGC 7 cut(s) 241, 349, 960, 1124, 1668, 2179, 2333
FokI GGATG 8 cut(s) 218, 1811, 1832, 2363, 2390, 2575, 2873, 2896
FriOI GRGCYC 3 cut(s) 31, 189, 1219
Fsp4HI GCNGC 7 cut(s) 241, 349, 960, 1124, 1668, 2179, 2333
FspBI CTAG 6 cut(s) 627, 791, 1728, 1734, 2463, 2784
GlaI GCGC 1 cut(s) 1166
GluI GCNGC 7 cut(s) 241, 349, 960, 1124, 1668, 2179, 2333
GsaI CCCAGC 2 cut(s) 1452, 1553
GsuI CTGGAG 1 cut(s) 296
HaeIII GGCC 9 cut(s) 567, 665, 680, 1117, 1577, 1872, 2178, 2450, 2556
HapII CCGG 3 cut(s) 41, 776, 845
HgaI GACGC 2 cut(s) 232, 608
HhaI GCGC 1 cut(s) 1167
Hin6I GCGC 1 cut(s) 1165
HinP1I GCGC 1 cut(s) 1165
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 494
HpaII CCGG 3 cut(s) 41, 776, 845
Hpy166II GTNNAC 6 cut(s) 156, 169, 1429, 2884, 2944, 3060
Hpy8I GTNNAC 6 cut(s) 156, 169, 1429, 2884, 2944, 3060
Hpy99I CGWCG 3 cut(s) 173, 185, 230
HpyCH4III ACNGT 5 cut(s) 909, 1238, 1390, 1639, 2065
HpyCH4IV ACGT 7 cut(s) 75, 158, 1272, 1543, 1701, 2068, 2755
HpyF10VI GCNNNNNNNGC 3 cut(s) 1123, 1486, 2488
HpySE526I ACGT 7 cut(s) 75, 158, 1272, 1543, 1701, 2068, 2755
HspAI GCGC 1 cut(s) 1165
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Ksp22I TGATCA 1 cut(s) 415
LguI GCTCTTC 1 cut(s) 711
LmnI GCTCC 2 cut(s) 192, 1214
Lsp1109I GCAGC 4 cut(s) 227, 971, 1110, 1679
LweI GCATC 4 cut(s) 1334, 1780, 2341, 2986
MabI ACCWGGT 1 cut(s) 1314
MaeI CTAG 6 cut(s) 627, 791, 1728, 1734, 2463, 2784
MaeII ACGT 7 cut(s) 75, 158, 1272, 1543, 1701, 2068, 2755
MaeIII GTNAC 7 cut(s) 738, 805, 943, 1595, 2147, 2779, 2867
MfeI CAATTG 1 cut(s) 1497
MflI RGATCY 5 cut(s) 34, 49, 874, 1301, 2465
MhlI GDGCHC 4 cut(s) 31, 189, 1187, 1219
MlyI GAGTC 7 cut(s) 174, 392, 535, 1614, 2320, 2482, 2900
MmeI TCCRAC 6 cut(s) 420, 459, 1446, 2029, 2312, 2336
Mph1103I ATGCAT 1 cut(s) 391
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 2 cut(s) 2271, 3056
MslI CAYNNNNRTG 2 cut(s) 1190, 2926
MspA1I CMGCKG 3 cut(s) 962, 1283, 1670
MspI CCGG 3 cut(s) 41, 776, 845
MspR9I CCNGG 8 cut(s) 777, 1113, 1162, 1316, 1439, 1526, 1952, 2925
MunI CAATTG 1 cut(s) 1497
Mva1269I GAATGC 3 cut(s) 389, 2090, 2596
MvaI CCWGG 7 cut(s) 1113, 1162, 1316, 1439, 1526, 1952, 2925
MvnI CGCG 1 cut(s) 744
MwoI GCNNNNNNNGC 3 cut(s) 1123, 1486, 2488
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 777
NcoI CCATGG 2 cut(s) 1600, 2696
NdeI CATATG 2 cut(s) 949, 1069
NgoMIV GCCGGC 1 cut(s) 40
NlaIV GGNNCC 3 cut(s) 305, 1303, 2210
NmeAIII GCCGAG 1 cut(s) 1198
NmuCI GTSAC 4 cut(s) 1595, 2147, 2779, 2867
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 2 cut(s) 2148, 2575
NspV TTCGAA 1 cut(s) 1289
OliI CACNNNNGTG 1 cut(s) 2926
PaeR7I CTCGAG 1 cut(s) 30
PagI TCATGA 2 cut(s) 2322, 2841
PciI ACATGT 1 cut(s) 2144
PciSI GCTCTTC 1 cut(s) 711
PcsI WCGNNNNNNNCGW 3 cut(s) 177, 231, 240
PctI GAATGC 3 cut(s) 389, 2090, 2596
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 2 cut(s) 2271, 3056
Pfl23II CGTACG 1 cut(s) 72
PflMI CCANNNNNTGG 1 cut(s) 1321
PkrI GCNGC 7 cut(s) 242, 350, 961, 1125, 1669, 2180, 2334
PleI GAGTC 7 cut(s) 173, 392, 535, 1613, 2320, 2481, 2900
PpsI GAGTC 7 cut(s) 173, 392, 535, 1613, 2320, 2481, 2900
Ppu21I YACGTR 2 cut(s) 76, 1702
PscI ACATGT 1 cut(s) 2144
PshBI ATTAAT 1 cut(s) 971
Psp124BI GAGCTC 2 cut(s) 189, 1219
Psp1406I AACGTT 1 cut(s) 1272
Psp6I CCWGG 7 cut(s) 1111, 1160, 1314, 1437, 1524, 1950, 2923
PspFI CCCAGC 2 cut(s) 1448, 1549
PspGI CCWGG 7 cut(s) 1111, 1160, 1314, 1437, 1524, 1950, 2923
PspLI CGTACG 1 cut(s) 72
PspN4I GGNNCC 3 cut(s) 305, 1303, 2210
PspPI GGNCC 9 cut(s) 905, 997, 1115, 1312, 1844, 1870, 2176, 2449, 2554
PstI CTGCAG 2 cut(s) 1182, 2175
PsuI RGATCY 5 cut(s) 34, 49, 874, 1301, 2465
PvuII CAGCTG 3 cut(s) 962, 1283, 1670
RsaI GTAC 8 cut(s) 74, 218, 379, 1368, 1428, 1530, 2067, 2788
RsaNI GTAC 8 cut(s) 73, 217, 378, 1367, 1427, 1529, 2066, 2787
RseI CAYNNNNRTG 2 cut(s) 1190, 2926
SacI GAGCTC 2 cut(s) 189, 1219
SalI GTCGAC 1 cut(s) 167
SapI GCTCTTC 1 cut(s) 711
SatI GCNGC 7 cut(s) 241, 349, 960, 1124, 1668, 2179, 2333
Sau96I GGNCC 9 cut(s) 905, 997, 1115, 1312, 1844, 1870, 2176, 2449, 2554
SbfI CCTGCAGG 1 cut(s) 2175
ScaI AGTACT 2 cut(s) 218, 2788
SchI GAGTC 7 cut(s) 174, 392, 535, 1614, 2320, 2482, 2900
ScrFI CCNGG 8 cut(s) 777, 1113, 1162, 1316, 1439, 1526, 1952, 2925
SdaI CCTGCAGG 1 cut(s) 2175
SduI GDGCHC 4 cut(s) 31, 189, 1187, 1219
SexAI ACCWGGT 1 cut(s) 1314
SfaNI GCATC 4 cut(s) 1334, 1780, 2341, 2986
SfcI CTRYAG 5 cut(s) 888, 1178, 2171, 2316, 3042
Sfr274I CTCGAG 1 cut(s) 30
SfuI TTCGAA 1 cut(s) 1289
SinI GGWCC 4 cut(s) 905, 997, 1312, 1844
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 2 cut(s) 1190, 2926
SmlI CTYRAG 4 cut(s) 30, 457, 1975, 3005
SmoI CTYRAG 4 cut(s) 30, 457, 1975, 3005
SpeI ACTAGT 1 cut(s) 2783
Sse8387I CCTGCAGG 1 cut(s) 2175
SsiI CCGC 5 cut(s) 348, 402, 2179, 2332, 2803
SspI AATATT 2 cut(s) 1373, 1779
SspMI CTAG 6 cut(s) 627, 791, 1728, 1734, 2463, 2784
SstI GAGCTC 2 cut(s) 189, 1219
StyD4I CCNGG 8 cut(s) 775, 1111, 1160, 1314, 1437, 1524, 1950, 2923
StyI CCWWGG 5 cut(s) 516, 660, 1297, 1600, 2696
TaaI ACNGT 5 cut(s) 909, 1238, 1390, 1639, 2065
TaiI ACGT 7 cut(s) 78, 161, 1275, 1546, 1704, 2071, 2758
TaqI TCGA 6 cut(s) 31, 163, 168, 270, 291, 1289
TatI WGTACW 4 cut(s) 216, 377, 1366, 2786
TauI GCSGC 3 cut(s) 351, 2181, 2335
TscAI CASTG 2 cut(s) 1393, 1600
TseFI GTSAC 4 cut(s) 1595, 2147, 2779, 2867
TseI GCWGC 4 cut(s) 240, 959, 1123, 1667
Tsp45I GTSAC 4 cut(s) 1595, 2147, 2779, 2867
TspGWI ACGGA 2 cut(s) 60, 1103
TspRI CASTG 2 cut(s) 1393, 1600
Van91I CCANNNNNTGG 1 cut(s) 1321
VpaK11BI GGWCC 4 cut(s) 905, 997, 1312, 1844
VspI ATTAAT 1 cut(s) 971
XagI CCTNNNNNAGG 4 cut(s) 486, 1935, 2255, 2456
XbaI TCTAGA 1 cut(s) 790
XceI RCATGY 2 cut(s) 2148, 2575
XcmI CCANNNNNNNNNTGG 1 cut(s) 2102
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 2 cut(s) 168, 2943
XmnI GAANNNNTTC 2 cut(s) 2271, 3056
XspI CTAG 6 cut(s) 627, 791, 1728, 1734, 2463, 2784
ZrmI AGTACT 2 cut(s) 218, 2788
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.